CN1292066C - 与人肺癌变相关的基因 - Google Patents
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- CN1292066C CN1292066C CNB021240787A CN02124078A CN1292066C CN 1292066 C CN1292066 C CN 1292066C CN B021240787 A CNB021240787 A CN B021240787A CN 02124078 A CN02124078 A CN 02124078A CN 1292066 C CN1292066 C CN 1292066C
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Abstract
本发明公开了与人肺癌变相关的基因。与人肺癌变相关的基因，由序列表中序列1-12的抑癌基因群及序列表中序列13-26的促癌基因群组成。本发明利用临床低分化肺鳞癌组织以及癌旁支气管上皮组织，构建了低分化肺鳞癌差别基因表达的抑制性消减杂交cDNA文库，并从中筛选出与肺癌病变相关的新基因，对于寻找参与肺癌恶性转化、肺癌诊断及其它肿瘤相关基因的发掘具有重要意义，同时也为肺癌发病机理研究及药物靶标的寻找奠定了基础。
Description
技术领域
本发明涉及与人类脏器癌变相关的基因，特别是与人肺癌变相关的基因。
背景技术
人肺癌变是一个涉及多种基因及其产物相互作用的复杂过程。寻求人肺癌发生发展过程的关键基因，将有助于探讨其癌变的内在分子生物学机制，并将对肺癌的预防、诊断和治疗产生重要的意义。人肺癌变关键基因的寻求不仅依赖于对现有已知基因功能研究的不断深化，还依赖于对其相关未知新基因的探索。人类基因组草图表明，人体大约有3-4万个基因。就现有肺癌相关基因的数量来说，对于科研和应用还是不够的，探索更多人肺癌变相关基因仍是我们目前亟需解决的问题，将为我们最终获得肺癌变相关的关键基因提供基础。
发明内容
本发明的目的是提供与人肺癌病变相关的基因。
本发明提供的与人肺癌病变相关的基因，是序列表中序列2的核苷酸序列。
本发明利用临床低分化肺鳞癌组织以及癌旁支气管上皮组织，构建了低分化肺鳞癌差别基因表达的抑制性消减杂交cDNA文库，并从中筛选出与肺癌病变相关的新基因，对于寻找参与肺癌恶性转化、肺癌诊断及其它肿瘤相关基因的发掘具有重要意义，同时也为肺癌发病机理研究及药物靶标的寻找奠定了基础。
具体实施方式
实施例1、细胞总RNA的提取
细胞总RNA的提取包括以下步骤：
1、低分化肺鳞癌肿瘤组织，通过微切割方法，取肿瘤纯度大于85％的标本部分进行总RNA提取；
2、从癌旁支气管上皮刮取鳞状上皮细胞，取细胞纯度大于85％的标本进行总RNA的提取；
3、总RNA提取方法用Trizol(Cat#155096-026，美国Gibico公司)试剂，按试剂说明书进行。
实施例2、抑制性消减杂交
按SMARTTM cDNA Synthesis Kit(Cat# k1052-1，美国CLONTECH公司)和ClontechPCR-SelectTM cDNA Subtractive Kit(Cat# k1804-1，美国CLONTECH公司)说明书进行操作。
获得抑癌基因克隆的消减方案为：以低分化肺鳞癌组织标本的cDNA为驱动方，癌旁支气管上皮细胞的cDNA为检测方，进行消减杂交。
获得促癌基因的消减方案为：以癌旁支气管上皮细胞的cDNA为驱动方，低分化肺鳞癌组织标本的cDNA为检测方，进行消减杂交。
实施例3、消减cDNA文库的构建(T/A载体克隆法)
经过常规PCR扩增的差减基因产物进行纯化后，分别取出3μl，与1μlpGEM-T载体连接，4℃过夜。从连接载体中取4μl转化DH5α受体细菌，接种于X-gal/IPTG/Ampcilin琼脂平板上培养过夜，挑取单菌落于加有1mlLB培养基的96孔板中，37℃振摇培养过夜。吸取80μl菌液，加20μl甘油，混匀，-70℃保存。其余用于测序。
实施例4、文库的测序
文库的克隆经扩增后提取质粒，采用pUC/M13下游引物、Megabase测序仪对插入片段进行单向测序，共获得883个核苷酸片段的序列。
实施例5、新基因的获得
将测序所得序列采用BLAST(http：//www.ncbi.nlm.nih.giv/blast/)方法与美国国家生物技术信息中心(National Centre for Biotechnology Institute，NCBI)已知功能核酸库(Nr库)进行比较，确定新基因序列，它们是序列表中序列1-12的抑癌基因群及序列表中序列13-26的促癌基因群
实施例6、本发明抑癌基因群在肺癌变中的作用
将抑癌基因群的每个基因分别构建表达载体并转染肺癌细胞系，并以转染空表达载体的肺癌细胞系做对照进行研究，绘制生长曲线表明，这些基因的表达都具有抑制生长的特性；进行软琼脂实验，计算克隆形成率，这些基因的表达能够明显不同程度地降低克隆形成率；将转染抑癌基因群基因的肺癌细胞分别接种裸鼠，与转染空载体的肺癌细胞相比，这些基因抑瘤效应显著。
实施例7、本发明促癌基因群在肺癌变中的作用
将促癌基因群的每个基因分别构建表达载体并转染永生化支气管上皮细胞系，并以转染空表达载体的永生化支气管上皮细胞系做对照进行研究，绘制生长曲线表明，每个促癌基因群的基因都具有促进生长的特性；软琼脂实验表明，克隆形成率明显增高；将转染抑癌基因群基因的肺癌细胞分别接种裸鼠，与转染空载体的肺癌细胞相比，这些基因的致瘤效应显著，而相应的转染空载体的肺癌细胞不致瘤。
序列表
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<213>人属人种(Homo sapiens)
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ctgaattatt ataatagtta cccactggtc ttcctttcct caggtttccc ctactactgc 60
cacatcaatc ttcttaaaac acttttctgc ataaattaag ctctaggtca aagactttgt 120
ttctccttga tcttgaaggc caagcactta gattttctcc ctcggcctgt cataatatgg 180
ccataggcta catccccaat gatggcaaac cacaaaatat tttccatggt gcattcacat 240
gatctgtttt gtatttgaaa tggttattct ggaggttg 278
<210>2
<211>192
<212>DNA
<213>人属人种(Homo sapiens)
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ctgtagtctc agccaccagt gaggctaagt gtgggaggag gcccgatccc agggatgttt 60
gaggctgcag tgagctggag tgcagtgaca tgatcacaga tcactgcagc tttcagtttt 120
aaaacagctt ctattacatt ttcttggtgg aagctgtatt tctaccttag tacctcggcc 180
gcgaccacgc ta 192
<210>3
<211>132
<212>DNA
<213>人属人种(Homo sapiens)
<400>3
gcgggaagca gtggtatcaa cgcagagttg ataagcagtg gtatcaacgc agagttgata 60
agcagtggta tcaacgcaga gttgataagc agtggtatca acgcagagta cctgcccggg 120
cggccgctcg aa 132
<210>4
<211>289
<212>DNA
<213>人属人种(Homo sapiens)
<400>4
tttttttttt ttttttttct ttttttgtga acttttacag tgacagaaac caattaaatg 60
cagtttttcg caaatcagaa gtggctgcat acacagcaga tttctgcaag aattactgta 120
tatatgtaga ctacattcca gtgcccagga aaaatcatct accttgcaac tttcttcctg 180
attttgaaca gacataatgc ttgaaacaaa atctctcact acgtccatta aagctttcaa 240
gcaggccatg tttaatttgg cagcctcccc aaggaccagt aaagccagt 289
<210>5
<211>246
<212>DNA
<213>人属人种(Homo sapiens)
<400>5
tttttttttt tttttttttt tttccccaac cccttgtttc tcctcctttc cccatcctca 60
cacccatctc cctccatttt ttctcactct cttttctcta tccctgtcct ggctatagct 120
acactatagc tcaaaacatc tagtcagcaa aattcataga acagtgaaaa gctagggaaa 180
aaatgccaca tggtgtggcc aagttgaaaa tggccatata gtacctgccc gggcggccgc 240
ttcgaa 246
<210>6
<211>338
<212>DNA
<213>人属人种(Homo sapiens)
<400>6
gaaatgatac ggaaaaaggc ataaaacccc caaaattcca actgagtttg gacatcactg 60
ggcagctgca gtgagagatg aagttgatct ttcgttgact aactcaacac atatttatca 120
aacacctgtt atgctctagt gactctataa ggtgctatta cagcagtgaa aatgatgact 180
tgaaatctat gacttgaaat ctctcctcag ggagcttcca taccagtggg agagatatac 240
aacaaaacaa aattaagtgt taaaatgtgt tttcttacat agtgttatgt tttcaggaga 300
agcaatgacg gaagccagag agaggacaca gttgtgta 338
<210>7
<211>431
<212>DNA
<213>人属人种(Homo sapiens)
<400>7
tttttttttt tttttttttt tagggaaggt agttgagata acctttccag atgttcaatt 60
gagtccgatc attttgtcag ctaacaaatg atgggaacag attaaggttc agctctggct 120
gctgttcagt ggctcagtca aaggatgggg gttgaagacc ttgaaacttc tgctatgaaa 180
gagggcaact gcacatgtgt ggctcacttt agctagtcct gaggcgtagg cgtgctccag 240
agctctgcgg caagtgtcag gttgtcagct ccatcctggt aattctggac aatgcttgtt 300
agccatagat ggacccggag gaaagcggtc tctcaaggct tgtgtgcacc aagcttgctg 360
cttaaagttt gttctttcca gaatgtgact gttaattagg taatgagtac ctgcccgggc 420
ggccgctcga a 431
<210>8
<211>540
<212>DNA
<213>人属人种(Homo sapiens)
<400>8
aggcttgaat ctctcctgta gaggaatttt aggattcttc tgcttacttc tttgatttct 60
ttcaggttgt gtcatatagt tgaaacagag ttaagattag ttgagtaatt agcttgctct 120
cattgactat ttcatttcaa aaacagcaaa gccccacata attgtgattg atcatctatt 180
taaaggaagt tacctaattt gccacttcta ccctttagtc tacttcctta ttacacatta 240
aagtcttcac tggtcatttt tttctttaaa agaaacacaa agagaaaggg tattaaataa 300
ataattgtat aggtttagta tttgcttgtt atttcctaaa agatgaagtc aagaggaaat 360
gttagcattc ccaacaaatg tttacatagt agaaatgact cttagaattc ttacaactgc 420
agagaacatt ttattttggc tgacaggaac accaagtatt ttccaaatgt ttctactcct 480
tactctaagc attgccaatg ctaatctact cacacagttt ataattattt gtcctcaaaa 540
<210>9
<211>380
<212>DNA
<213>人属人种(Homo sapiens)
<400>9
tgaggcatta ctaacctgtc ttgattggta acgcacattc cttcaaacta gctcatccca 60
ccaaatctta aacacatgca caccccactt ctttcatgtt tgcccttgta gtcacaaatg 120
ggaaatgaag ttgcctctca tttggaggca taccagctga gagaggatag gaacactctg 180
tcaaaaacaa atgccaagct ctgctaaact catcagttaa ccttactaaa cgtaagtaac 240
aaagaaatgc cttctttagc ttgataacac tccctatgga agttaacatc tgtcttgatt 300
tagctttctg caaataggtg caactcctta taaatgccgg ggcaaagatg gccaaccacc 360
tgcccgggcg gccgctcgaa 380
<210>10
<211>223
<212>DNA
<213>人属人种(Homo sapiens)
<400>10
tttttttttt ttttttgcca ctattatcta tcacaaatgt caatcatgac cataccttgc 60
ttaagttggt ttcccttgcc aagagcactg tttttcctat acctgttgaa attttggaaa 120
tccaattcta cctcctctct tcccttaagc atctcttcct tcccttctcc ccaaatttat 180
atttagtcac atatattatc gtacctcggc cgcgaccacg cta 223
<210>11
<211>180
<212>DNA
<213>人属人种(Homo sapiens)
<400>11
ttgtgtagga tagctgtcca gaggtggaat gctggctctg aggctgaaga tttcagatgc 60
tggcaggtat ttccaaattg ctctccaaag agcttactcc cattgatatc aacagcatct 120
gagaggacat tttgattcat atgtggtttt tccagtgggg tacctcggcc gcgaccacta 180
<210>12
<211>449
<212>DNA
<213>人属人种(Homo sapiens)
<400>12
tgcccgcccg ggcaggtaca aggggaaaat acaagcctta gggaaaagat gacttgctta 60
aggtagtaag gtaatacagc cgtacctatg aaaacgaaag aatagcactg tgggctgaaa 120
accagaggaa ctttctccaa aatgatacat cattttagct tagatttaga aagggttaag 180
agggtcccac ctttgtcttt aggaatagat attttcttac aaagctaaat gccattatca 240
cacctgtcaa gattaacaat aaactggttt gttaaatcag gatccaaaca cacttatgtc 300
tcttaagtgt cataatctag agcagtctct ctcccttttt taccttcaca acatggactt 360
acaacagtaa ctgggtcagc tgctctgtaa aatgtcctac attctggatt tgtctattta 420
cttccttaag ttgtaatctg acttactcc 449
<210>13
<211>552
<212>DNA
<213>人属人种(Homo sapiens)
<400>13
tagttctaaa cagcaaccaa gttattacca gaagtagtcc atatattgta atcttgatga 60
ttgaagtgtt acgttcactt ngggtgttaa ctctcactgt tgaagaagtg accattattt 120
taagatgatt tacttcctga cttaaatata atatagcagt agatctttga gacaatgctt 180
ttttaatatt gtcttcttta atctttttta ttatgtttgg gctctttgtt tttgacaggc 240
cttaggctac tttcacaggg cagaacaagg taaggtattt ttgattacac atattaatat 300
agttaaaatt gtatgaatgc tgaatatata ctataatagc aatgtctaaa gcagttgttt 360
attctgttat acttattttc gtgctagata ctgtaaaaca atttttaagg tctttattca 420
gtaaagatca cctgaatgag aaataggctg ctactgaact tagagctgca gttctggaat 480
gtctatcatt tgatgtaatt ctgcttcctt tgtgagagca gtctctgaca tggtaattgt 540
ctcattcaag ac 552
<210>14
<211>576
<212>DNA
<213>人属人种(Homo sapiens)
<400>14
acttttctca taaagtttag cctacagaaa ctatcgccac ccaaattaaa catcacccaa 60
gctaatattc tttcctcctt ctaaagatga gctagcgaaa ctttttatag gttgtccctt 120
taatgcagct ttttagaata aacattttta cattttttct taaaagaatt attttttgaa 180
gtctgaggaa aaatccgctt gcctagtgaa tttggcacac acagagtaac aacaaatcaa 240
actttaagct agcaaccaac acacaaaata agcatgcaag gaatagaata agttttatat 300
ggataaggta ttttagccaa ctccacttat aaggtattac aaaatctcta tatngttttg 360
aagctatgtg tcgcagttta aagttacttt taacaataat acgtatattt acaattgact 420
taaaaaacta ttttcaagga agttagaaac ctatggcaca ccaacgcatc ttctggaaaa 480
tgaagacgat acaatgtcat gtggcaagtt tcaatatatg aaggactaga aaaaatattt 540
ctttactcaa ttctgaatta caagttcata ataatt 576
<210>15
<211>455
<212>DNA
<213>人属人种(Homo sapiens)
<400>15
cactcacata taccaccatc ttatctcatc ctcacttatg gtaagtgctt ctgttattca 60
ttttatagac gaggaaacta aggagaagag aagttaagta acttgcccag gttacacaat 120
aggaagtaat gaataatgga tccaggattt aaagccagta gtctgaccac tgagcacaat 180
taattaattt taactacaaa atctcaccat atggttaaat gaatacctca ttcttgtgta 240
gtagtttaca gttacaaatt ggtgagtatc ctcattttcc atattagaaa caaaggcatg 300
aaaatgttaa gagatttggc aaggttatgt agccattttt caggcgaaag aatattagga 360
acttggtctt tggattctcc cgctcacaaa caaaatctaa acttgaactg tatgcaacta 420
tctctgctat ggcagtcatg cacctagaac ctggt 455
<210>16
<211>261
<212>DNA
<213>人属人种(Homo sapiens)
<400>16
tttttctttc tttctctttt tttttttttt tcaaaaagct cttattgaag cccttagggc 60
agtgatcctc aaagtatagc tctgagacca gtcccatgag catcacctgg aaacatgctg 120
gaaatgcaaa tttccaagcc caactccata catacaaatt tccaagccca actccccaca 180
tccataggat gtggataaac tagaatagaa acttcgtgtg ggtggggccc caaaacctgt 240
tttttcagca aactcttctg t 261
<210>17
<211>280
<212>DNA
<213>人属人种(Homo sapiens)
<400>17
tggcaatgca gtgccaatct ccctctgtta ctgttccctc tagtgcctgg ggttggggca 60
ggtgcagggg tgtggggaag gagagagaag caatggggaa acaggtgttt ctctacttaa 120
gacttcatga acacatacag tcttgaaatg gctagaaaag caacaactac aatgataaaa 180
atcaacagat caattgattt aggcatttca gcagcacccc ttccttttct ttcctgggta 240
tctagcagta gatattaatt agagataccc aaagaattta 280
<210>18
<211>551
<212>DNA
<213>人属人种(Homo sapiens)
<400>18
agcatgcatt ttgggcatgg tgccatgctg acctttcccc tttaatgtgt agtgccccat 60
gcttcactta gtattttggc agcctgcaca cagaggctag catttgagcc cagtgctttg 120
aagagagcaa aaaagtttca gaatgagaac tcttcaagtc actggttata catagggtat 180
tatctcccac gtcaatggag caatggatga cctgcctggt ttttttttag gcctcttccc 240
ttttgtcttt gcttcaaaaa aaaattaata gattaaatta tttgtcatcc aagagaagta 300
aacaggtcag atcctatgtt ccatatcatg ggattgtaaa cgctcatatt tccttttgtg 360
aaaatttttt ctttaagagc tgccaagcat gaacatactg cattcactaa tattctctgt 420
catcatgcta ctgttgattg aaaggagaag aacctacttg caccccagcc aagggtgagg 480
tgtcctgggg agtgtcacac ttttgtgtta tactgttaac aagttcatgt gtaatgtaat 540
aataaatcat t 551
<210>19
<211>212
<212>DNA
<213>人属人种(Homo sapiens)
<400>19
ccaaggaagc agcacattca ggctgagtat ataataacag gagagccatt cgcactcctg 60
tcatcacaaa agaaaatact gcccttgcat aaatctccat tgtgttttat acagcattcc 120
agaaatggtg caggaggcaa ttcaaatgaa tagaaggaga atttggtggc aggggggtga 180
ggggaagggt ttgtagactt gtacctgccc gg 212
<210>20
<211>529
<212>DNA
<213>人属人种(Homo sapiens)
<400>20
tcgagcggcc gcccgggcag gtactggggg gatgcacttg agtgccattt ctgatactgt 60
cattgtatga ctgctgatgc tcgtttatgt tgttctttgg aagctatagt gagcctgatg 120
ttttcacatt ttaaaaaatg cttcctgaga cttctttaag ctcctctaac ttcttcagtg 180
catttgggat caaacttagc tcttagatca gcaaacctga cttgcttcta cttttgcaat 240
ttttcaggcc ttaccatatt tcttatctct gtcttcccac ctgataggaa aatcttgcag 300
atttgaaaca gtggtttctg gcctgctgct gcagcaaatt ccctatgttt atttaagaag 360
tcgggcctat gcttttaacc tacaaatgat gagtaattta cttgcaatga acacaggtag 420
cctgctgctg tttataacct gttgaggata tttatagcag gcaggaggca ggcctagtgt 480
gttattgaca ggtgacttta gagaacgtac ctcggccgcg accacgcta 529
<210>21
<211>555
<212>DNA
<213>人属人种(Homo sapiens)
<400>21
ctggcataga gtaaaaatga ataaaagtta gctagtaata ttaatgcaaa tcattatgcc 60
aaaacttaag gataggagga acaagaagcc ctagtctcaa tattactgct taaaatactg 120
gaaagtcatc agacctatca aatgatggga tgtcaaacct tccatttcca gagtggaagg 180
gaagctacgt gagaaaggaa gaaaacacat cccgcctaca gtttaataat tttataatat 240
agttgaattt aataatactt caacaggtta tcttagttaa gaagaataga caaggcatat 300
aaactgctat gtaagattag caacacctgg ataagtgaaa ccatgaagtg tctcagaaga 360
ctgaaagaaa tgtagtgtta cctgaagaca aaacggattg aattttctta tagagcaaac 420
cagcagtaag accagtaagc aacttaactg tgtttccaac aacaaccaga aatgggttaa 480
aatgatacac acattctgac acagttccct ctctcaagag ccatactgga catagaatta 540
agttaagcaa atcat 555
<210>22
<211>298
<212>DNA
<213>人属人种(Homo sapiens)
<400>22
ttatgttagg taatatttaa aaattgatta ttgaagtata gatttggtca cttgtatctc 60
tccttaattg taatagcaat agtctgatag ggaagtggca taatgtagtg aagagaacct 120
gggctttgga gttgggacaa cctgggttca gatgctggct ttcttgctta ttatctgtgt 180
aactttgggc aaattaccta acatttctaa gttttctatt ctataaattg tctttgataa 240
gctggcaagg ttcaagtgag atgatgcatt taagtggttt accatctaaa aggtacct 298
<210>23
<211>481
<212>DNA
<213>人属人种(Homo sapiens)
<400>23
acagcagggc tgagattatg ggctctggcc agactgatgt gactgaattc ctgattcagt 60
cacatagtgg tgtgaccttg aggacattca cctaaccttt ccaggactta atattgaacc 120
tgccttctgt gaggcggttt gaagactaac atggtaacat gaaactgaca catctagaag 180
aatgcctggc agaaggcaga cactgagtgt tagttggtat tattgcacag tgcaaccaat 240
gctatgctgg cataagccga ccgcactact acgaaattag cttcctgcta accaagcttg 300
gtccacccag gagggctccc tgaggaggtg acctctaagg atgaacaaga cttaggtgaa 360
agtagagcag aatatgagga gaaattgaat atccagtctc aaaataaaac acgcagatgt 420
gtagagcaat tttctacaaagtgcatgcag catttaaaca atgccataaa gaagctggct 480
c 481
<210>24
<211>604
<212>DNA
<213>人属人种(Homo sapiens)
<400>24
tgagatatac ttacagagtg ggtctgctct tttccattgt tttccattct atcttagttt 60
aattgatttt tatggagcag aaatttgagg gcttttgaat acaattaaac ccatcagcct 120
tgtttataat atttttctat tgctctaaca ataagtcaca gtcctaaagc atgccctgcg 180
ctaaatatta ggcaacagac tagagggtct cattcaattc aacaaaggct ctagcccagt 240
tgcccttcac aatgtgtgta acaattcttt ttcatgtctt acaactttgt atcctttttg 300
taacctcaca tcctctttgt ttacagtata tggaatgacg catggatcta agtttattta 360
tcgccatacc agtaattagt atacctaaga aaaggggtct acaaactatg gcttgtggat 420
ttggcccact gcctatatct ataaataaagttctattgga acacagttca ctcacttgtt 480
tccatactgt ctgtggctgg tttcgcacta caaaagcaaa gctgagtcaa aaatacttac 540
tatctggccc tttacataaa gtttgtctac tcttacctaa aaccattgat cacataatta 600
ttcc 604
<210>25
<211>246
<212>DNA
<213>人属人种(Homo sapiens)
<400>25
taagaaaggt tacgtttttg cctaaggtca cacagttaaa aataagagcc agaatgggaa 60
tcagaaccag ctgtcactac tttttatacc gtaacactga ctctctaaat tttgctcccg 120
gtggaagaag ctcctaatgc ctcttaggtt acatccctca taggttacgt tcaaataacc 180
tatctcccac cacacaggac aggctagcta cacaagttat atccctcaag gtcattactc 240
aaattg 246
<210>26
<211>265
<212>DNA
<213>人属人种(Homo sapiens)
<400>26
ccatcctcca gaggaaattt tggctatgcc ttgtatgacc ccatataaac aagaaggagt 60
ctacaccctt cttatatgat tgtagcaggt taggaagaaa tatctggttc attactggga 120
aaaggagggc caatgtagga taggcattct aatcgattaa cttatatact tttgggaaat 180
gtttaggagg aagaatggaa ataaggatag ctaacattta ttgagcactt actttctgcc 240
aggtacctcg gccgcgacca cgcta 265
Claims (1)
1、与人肺癌病变相关的基因，是序列表中序列2的核苷酸序列。
Priority Applications (1)
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|---|---|---|---|
| CNB021240787A CN1292066C (zh) | 2002-06-18 | 2002-06-18 | 与人肺癌变相关的基因 |
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| CN1292066C true CN1292066C (zh) | 2006-12-27 |
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| CN101054413B (zh) * | 2007-03-05 | 2010-10-13 | 中国医学科学院肿瘤研究所 | 一种肿瘤相关蛋白及其编码基因与应用 |
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