CN1284567C - Medicinal liquid for treating osteoporosis and its preparation method - Google Patents
Medicinal liquid for treating osteoporosis and its preparation method Download PDFInfo
- Publication number
- CN1284567C CN1284567C CN 200410023729 CN200410023729A CN1284567C CN 1284567 C CN1284567 C CN 1284567C CN 200410023729 CN200410023729 CN 200410023729 CN 200410023729 A CN200410023729 A CN 200410023729A CN 1284567 C CN1284567 C CN 1284567C
- Authority
- CN
- China
- Prior art keywords
- radix
- medicinal liquid
- carapax
- alcohol
- parts
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Landscapes
- Medicines Containing Plant Substances (AREA)
Abstract
The present invention relates to a medicinal liquid for treating osteoporosis and a preparation method thereof. The medicinal liquid is prepared from traditional Chinese medicine as main components in a prescription which comprises herba epimedii, eucommia bark, manyprickle acanthopanax root, himalayan teasel root, fortune's drynaria rhizome, oyster, tortoise plastron, tuber fleecedlower root, milkvetch root, red sage root, dried old orange peel and prepared rhizome of rehmannia rhizoma through aqueous extraction-alcohol precipitation technology. Effective components of substances with androgen-like effects, substances with estrogen-like effects, substances with effects which are similar to reproductive hormone-like effects, etc. are firstly obtained through mixing extraction, tortoise glue and chondroitin sulfate are extracted from the tortoise plastron later so as to obtain effective components, and then the two effective components are mixed and seasoned with stevioside so as to obtain a liquid preparation. The storage shelf life of the medicinal liquid is one and a half years at normal temperature. Clinical outcomes show that patients of which the symptoms of lumbar and back pain are obviously lightened or relieved account for 91.23% of osteoporosis patients treated by the medicinal liquid.
Description
(1) technical field
The present invention relates to a kind of prescription and preparation method of Chinese medicine, be specifically related to osteoporotic medicinal liquid of a kind of treatment and preparation method thereof.
(2) technical background
For osteoporosis, many at present employing hormones and some other Western medicine are treated, as calcitonin, estrogen, androgen, calcium preparation, fluoride, Diphosphonate, V
D3, thiazide etc., these medicines all have certain effect to the treatment of osteoporosis.But some drugs can produce some side effect in treatment, as estrogen is the choice drug of treatment postmenopausal osteoporosis, but this medicine is taken the generation that can bring out uterus carcinoma, breast carcinoma for a long time, in case stop using the bone amount is lost fast, open up man, Qiao Huayi referring to Teng Jia. osteoporosis and curative thereof. the Japan medical science introduction, 1990,11 (9).Wherein the scope of application of some drugs has some restrictions, only is applicable to male's osteoporosis as androgen and molecule derivant thereof; Diphosphonate is applicable to postmenopausal women's osteoporosis; And calcitonin and phenothiazine drug are mainly effective to the osteoporosis that glucocorticoid causes, referring to Zhu Jianmin, Jin Zongda. osteoporotic Drug therapy. and foreign medical science geriatrics fascicle, 1993,12 (6); Calcium treatment has conclusive effect to child and teenager osteoporosis prevention, but does not have immediately protective effect to suppressing postclimacteric bone loss.In addition, active vitamin V
D3The additional combined treatment of needs and calcium preparation.And some treat the low lattice costliness of osteoporotic medicine, have limited being extensive use of of these medicines.And the Chinese medicine osteoporosis adopts multiple therapy methods such as the kidney invigorating and essence nourishing, kidney-supplementing liver-boosting, strengthening spleen, tonifying kidney, invigorating kidney, promoting blood circulation, bone and muscle strengthening and invigorating the kidney and strengthening the bones more.
Because the sickness rate height of osteoporosis, had a strong impact on old people's health, at present, osteoporotic control more and more causes extensive concern both domestic and external, and existing many patent reports.CN1155430 discloses " a kind of treatment osteoporosis Chinese patent medicine and preparation ", this medicine is a main component with tens kinds of Chinese medicines such as animal bone, Carapax Et Plastrum Testudinis, Radix Rehmanniae Preparata, Cortex Phellodendri, Fructus Lycii, Radix Asparagi, and the tablet of making is 90.72% to the treatment total effective rate of renal deficiency type osteoporosis." phosphoric acid salt and the parathyroid hormone that are used for osteoporosis " of The Procter ﹠ Gamble Co's nineteen ninety-five application, notification number 1157566, this method comprises the diphosphonic acid salt with the chemical compound of anti-bone resorption, estrogen compound and estrogen antagonist chemical compound and the combined effect of thyroid parahormone reach the osteoporotic effect of control.Liu Haifeng application " medicine of curing osteoporosis " in 1993, notification number 1079160 is made up of 18 flavor Chinese herbal medicine such as Lignum Aquilariae Resinatum, Stigma Croci, this perfume (or spice), Rhizoma Chuanxiong, Ramulus Cinnamomi.Chinese and Germanic Union Research Inst.'s application " a kind of compound method of functional food of protect against osteoporosis " in 1992, notification number 1076088, what this method was made is to be a kind of granular health food of main effective ingredient with Fructus Lycii, Poria, Semen Euryales, Semen Nelumbinis, Rhizomadioscoreae etc.Medicine Tech. Development Centre, Zhanjiang Medical College 1992 application " goods of protect against osteoporosis and method for making thereof ", notification number 1080537, these goods are that the fruit extract with Umbelliferae Cnidium Cusson plant Fructus Cnidii is a main component.Medicines Science and Technology Development Center, Guangdong Medical College (2000 application " the new purposes of Radix Polygoni Multiflori and extract for treating osteoporosis thereof ", notification number 1306837, these goods are that the root piece with polygonum multiflorum thunb is the medicine that primary raw material is made.Medicines Science and Technology Development Center, Guangdong Medical College (2000 application " the new purposes of Radix Ginseng and extract of Radix Ginseng stem and leaf protect against osteoporosis ", notification number 1323588, these goods are that the dry root with the Araliaceae Radix Ginseng is a primary raw material.
(3) summary of the invention
The present invention is directed to the deficiencies in the prior art, osteoporotic medicinal liquid of a kind of treatment and preparation method thereof is provided.
Technical scheme of the present invention is as follows:
Osteoporosis is one of old people's commonly encountered diseases, and its sickness rate height has now had a strong impact on old people's health.The traditional Chinese medical science is thought " kidney governing bones ".Vital essence is full in the bone, the bone marrow of just supplementing nutrition, and bone marrow is full, and then sclerotin is hard.If asthenia of renal qi, the store essential substances deficiency, the kidney governing bones dysfunction, bone loses nutrition, skeleton fragility then, the osseous tissue amount in the function reduction of bone, unit volume reduces, and osteoporosis takes place.According to " kidney governing bones " this theory of medicine, with traditional the kidney invigorating, mend the prescription of bone and treat osteoporotic achievement in research in recent years and combine, with theory of Chinese medical science for instructing prescription.Form by Herba Epimedii, the Cortex Eucommiae, Radix Et Caulis Acanthopanacis Senticosi, Radix Dipsaci, Rhizoma Drynariae, Concha Ostreae, Carapax et Plastrum Testudinis, the Radix Polygoni Multiflori, the Radix Astragali, Radix Salviae Miltiorrhizae, Pericarpium Citri Reticulatae, Radix Rehmanniae Preparata.
The osteoporotic medicinal liquid of treatment of the present invention is made up of following component, is weight portion:
Herba Epimedii 9-15 part, Cortex Eucommiae 9-15 part, Radix Et Caulis Acanthopanacis Senticosi 0-15 part, Radix Dipsaci 10-15 part, Rhizoma Drynariae 0-15 part, Concha Ostreae 18-24 part, Carapax et Plastrum Testudinis 0-9 part, Radix Polygoni Multiflori 0-15 part, Radix Astragali 0-15 part, Radix Salviae Miltiorrhizae 15-30 part, Radix Ginseng 0-15, Pericarpium Citri Reticulatae 1-3 part, Radix Rehmanniae Preparata 15-24 part, Fructus Lycii 0-15, Radix Angelicae Sinensis 0-15.
Component of the present invention is preferably as follows:
One, Herba Epimedii 9-15 part, Cortex Eucommiae 9-15 part, Radix Et Caulis Acanthopanacis Senticosi 10-15 part, Radix Dipsaci 10-15 part, Rhizoma Drynariae 9-15 part, Concha Ostreae 18-24 part, Carapax et Plastrum Testudinis 6-9 part, Radix Polygoni Multiflori 12-15 part, Radix Astragali 12-15 part, Radix Salviae Miltiorrhizae 15-30 part, Pericarpium Citri Reticulatae 1-3 part, Radix Rehmanniae Preparata 15-24 part is weight portion.
Two, Herba Epimedii 9-15, Cortex Eucommiae 9-15, Radix Dipsaci 10-15, Concha Ostreae 18-24, Radix Polygoni Multiflori 12-15, Radix Salviae Miltiorrhizae 15-30, Radix Ginseng 6-15, Pericarpium Citri Reticulatae 1-3, Radix Rehmanniae Preparata 15-24.
Three, Herba Epimedii 9-15, Cortex Eucommiae 9-15, Radix Dipsaci 10-15, Rhizoma Drynariae 9-12, Carapax et Plastrum Testudinis 6-9, Concha Ostreae 18-24, Radix Ginseng 6-15, Radix Salviae Miltiorrhizae 15-30, Pericarpium Citri Reticulatae 1-3, Radix Rehmanniae Preparata 15-24, Fructus Lycii 12-15, Radix Angelicae Sinensis 12-15.
The flavoring agent that also has 1/300 weight portion in the osteoporotic medicinal liquid of above-mentioned treatment.
Above-mentioned flavoring agent is a stevioside.
This used medicine of writing out a prescription is a certified products through the crude drug expert testimony, and its kind is:
1, Herba Epimedii: Epimedium macranthum Morr.et Decne
2, the Cortex Eucommiae: Eucommia Ulmoides oliv
3, Radix Et Caulis Acanthopanacis Senticosi: Acanthopanax Senticosus Harms
4, Radix Dipsaci: Dipsacus asper Wall.
5, Rhizoma Drynariae: Psoralea corylifolia L.
6, Concha Ostreae: Ostrea ta.oemwjamemsia Crosse
7, Carapax et Plastrum Testudinis: Chinemys reevesu (Gray)
8, the Radix Polygoni Multiflori: Powgonum multiflorum Thunb
9, the Radix Astragali: Radix Atragali
10, Radix Salviae Miltiorrhizae: Salvia miltiorrhiza Bunge.
11, Pericarpium Citri Reticulatae: pericarpium Citri Reticulatae
12, Radix Rehmanniae Preparata: Rehmannia glutinosa Libosch.
The prescription foundation
Because deficiency of kidney-QI, deficiency of kidney-essence, bone marrow lose and fill, and hipbone loses supports, fragile unable, and osteoporosis takes place.As seen the traditional Chinese medical science low and imbalance of suffering from a deficiency of the kidney into allomeric function.Show as immunologic hypofunction, the going down of hypothalamic pituitary gonadal axis function, sex hormone level descends, and then causes the bone function reduction, and the osseous tissue amount in the unit volume is reduced, and bone density descends, and osteoporosis takes place.According to motherland's medicine principle, according to the experience of long-term treatment osteoporosis, collect many side chiefs, with the kidney invigorating and essence nourishing, the Chinese medicine of bone and muscle strengthening is main, suitably is equipped with the Chinese medicine of blood circulation promoting and blood stasis dispelling, and is pointed to osteoporotic treatment.It is the Therapeutic Principle that " the refreshing liquid of bone strengthening " prescription is complied with above-mentioned, with reference to classic of TCM record prescription, modern study achievement in conjunction with kidney-nourishing tcm drug, select for use Herba Epimedii, the Cortex Eucommiae, Radix Et Caulis Acanthopanacis Senticosi, Radix Dipsaci, Rhizoma Drynariae, Concha Ostreae, Carapax et Plastrum Testudinis, the Radix Polygoni Multiflori, the Radix Astragali, Radix Salviae Miltiorrhizae, Pericarpium Citri Reticulatae, Radix Rehmanniae Preparata to form prescription, these Chinese medicines cure mainly function and the modern study situation is as follows:
1, Herba Epimedii: property suffering, temperature.Return liver, kidney channel.Kidney invigorating and YANG supporting, strengthening the tendons and bones are arranged, the merit of expelling wind and removing dampness.Now research is known has the gonepoietic effect of promotion to animal.Prostate, seminal vesicle, act anus flesh are heavily increased, the effect of androgen sample is arranged.
2, Concha Ostreae: nature and flavor are salty, be slightly cold.Return liver, kidney channel.Include CaCO
3, trace element such as Mn, Al.Help protect against osteoporosis.
3, the Cortex Eucommiae: nature and flavor are sweet, temperature.Return liver, kidney channel.The merit that the kidney invigorating, bone and muscle strengthening, strong waist knee joint are arranged." herbal classic " " hard muscles and bones, is removed and is itched dribbling urination under cloudy strong will for main pain along the spinal column, invigorating middle warmer benefit vital essence." modern pharmacology proof can suppress the minimizing of bone mass.
4, Radix Salviae Miltiorrhizae: the nature and flavor hardship, be slightly cold.GUIXIN, heart born of the same parents, Liver Channel.The function that blood circulation promoting and blood stasis dispelling is arranged, modern pharmacological research show, promotes all to have shown good result aspect the regeneration of tissue and the reparation at microcirculation improvement.Its effective ingredient TANSHINONES plays estrogen-like effects, can increase the intestinal calcium absorption.
5, the Radix Astragali: sweet, the tepor of nature and flavor.Return spleen, lung meridian.Be the key medicine of QI invigorating, mend all empty deficiencies, and the effect of class reproductive hormone is arranged.
Herba Epimedii, property suffering, temperature are returned the Liver and kidney warp, have to mend the waist knee joint, strengthening the tendons and bones, the effect of wind-damp dispelling is controlled the disease of all cold wind consumptive disease, is we's key medicine; Radix Rehmanniae Preparata tepor, nourishing the blood and yin, vital essence replenishing and marrow benifiting and Herba Epimedii are we's monarch drug altogether.The Radix Ginseng strongly invigorating primordial QI, spleen reinforcing hemopoietic, supplementing QI for promoting the production of body fluid; The sweet temperature of the Cortex Eucommiae, the merit of tool invigorating the liver and kidney, for controlling lumbago due to renal deficiency, the key medicine of flaccidity and weakness of the lower limbs, with Herba Epimedii, Radix Rehmanniae Preparata, Radix Ginseng with better with effect, be we's ministerial drug altogether with Radix Dipsaci, Niu Xi, Rhizoma Drynariae, Carapax et Plastrum Testudinis, the Radix Polygoni Multiflori, Radix Et Caulis Acanthopanacis Senticosi, Concha Ostreae; The Radix Salviae Miltiorrhizae hardship, be slightly cold, blood circulation promoting and blood stasis dispelling improves circulation, and the hypoxia-bearing capability and the Pericarpium Citri Reticulatae that improve normal pressure and low pressure condition lower body are adjuvant drug altogether.
Above Chinese medicine, GUIXIN, liver,spleen,kidney, event heart master blood are human life activity's center.Blood stored in the liver, main muscle, the master takes charge of bending and stretching of whole body muscles and bones joint.The spleen governs the blood master's limb muscle is the foundation of acquired constitution.Kidney governing bones, living marrow, store essential substances are the source of reproductive development, and life originally." element asks that five hide a generation piece of writing " said: " bone of kidney also "." element asks normal person meteorological opinion " said: " the kidney providing essence-QI to the bone and marrow also ".As seen bone and marrow are the master of institute of kidney.Therefore, then skeletonization is living active, firm, strong strong for kidney qi kidney essense abundance.Otherwise then bone marrow loses and fills, and skeleton loses supports, fragile unable.Therefore when making up a prescription, select the Cortex Eucommiae, the Concha Ostreae of invigorating the kidney and strengthening the bones, the Radix Salviae Miltiorrhizae of blood circulation promoting and blood stasis dispelling for use, the Radix Astragali of benefiting QI and nourishing blood.Pharmacology proves that " the refreshing liquid of bone strengthening " prescription of being furnished with above-mentioned Chinese medicine truly has microcirculation improvement, increases immunologic function, and hormone-content in the increase serum, suppress or the hypokinetic generation of correction hypothalamic pituitary gonadal axis the absorption of bone density improving and intestinal calcium, thereby the process of delaying senility." the refreshing liquid of bone strengthening " to the targeted effect of sufferers of osteoporosis face, is a kind of desirable new drug that can improve senior health and fitness's level.
It is as follows that the present invention treats osteoporotic medicinal liquid preparation method, and wherein step 1 and 2,3 precedence are not limit:
1, adopt the technology of water extract-alcohol precipitation to produce the nothing alcohol concentrated solution of Chinese medicine: to take by weighing each component except that Carapax et Plastrum Testudinis by above-mentioned component weight portion, the water Rapid Cleaning secondary that adds qdx, the water logging that adds qdx was for the third time steeped 1.5 ∽ 2 hours, decocting is to boiling then, keeping little boiled 0.5-1.5 hour, filter out medicinal liquid, repeat decocting again and fry in shallow oil altogether three times, three times medicinal liquid merges, and leaves standstill, get supernatant concentration to 1/3 of amount of liquid medicine, again this concentrated solution is added 95% edible ethanol under fully stirring, reach 30% to alcohol concentration, left standstill precipitate with ethanol 3 hours, pour out supernatant, the residue sediment centrifugal clear liquid, with the precipitate with ethanol supernatant and centrifugal after clear liquid merge, steaming and decocting is to there not being alcohol again, ethanol reclaims, and must not have alcohol and concentrates medicinal liquid.
2, take by weighing Carapax et Plastrum Testudinis by above-mentioned component weight portion, be ground into fritter, decocting 2.5-3.5 hour, leach medicinal liquid, the nothing alcohol with above-mentioned gained concentrates the medicinal liquid merging again.The Carapax et Plastrum Testudinis granulated slag is stand-by.
3, above-mentioned Carapax et Plastrum Testudinis granulated slag is added the 2%NaOH and the 0.5%NaCL hydrolysis of 4 times of amounts, shook 20-25 hour down at 50 ℃, to the disappearance of granulated slag piece, regulate pH to 8.5 ∽ 9, this medicinal liquid layering gets the transparent supernatant of pale brown color.Add dehydrated alcohol to alcohol concentration and reach 30-90% in this supernatant, placement is 20-25 hour naturally, filters to get filtrate, and this filtrate is boiled off ethanol to there not being the alcohol flavor, gets the Colla Plastri Testudinis of sulfur acid chrondroitin.
4, Carapax et Plastrum Testudinis decocting liquid and the Colla Plastri Testudinis in the step 3 in the alcohol of the nothing in the step 1 concentrated solution, the step 2 are merged, add 1/300 weight portion stevioside seasoning,, left standstill 3-5 hour the medicinal liquid mix homogeneously, filter pressing then, canned, canned back was 110 ℃ of sterilizations 0.5 hour.
Above-mentioned filtering liquid medicine uses 80 purpose stainless (steel) wires.
The centrifugal of step 1 is with the centrifugal clear liquid that gets of link-suspended basket centrifuge 1400r/min.
When not containing Carapax et Plastrum Testudinis in the component, save step 2 and 3 in the above-mentioned preparation method.
Treatment osteoporosis medicinal liquid chemical composition analysis, pharmacological research, toxicity test, clinical observation and stability study have mainly been carried out.Treatment osteoporosis medicinal liquid mainly with icariine as quantitative target, adopt thin layer chromatography scanning can fast, accurately measure the content of icariine, the average content of icariine is 6.23% in this product.By the SD rat experiment, pharmacology to treatment osteoporosis medicinal liquid is studied, find that treatment osteoporosis medicinal liquid can reduce the excretion of urine calcium and urinary hydroxyproline, and urine calcium mainly decomposes from bone mineral, urinary hydroxyproline mainly decomposes from bone matrix, explanation thus, treatment osteoporosis medicinal liquid can suppress bone inorganic salt and organic minimizing; Treatment osteoporosis medicinal liquid has also improved the content in tibial bone ore deposit, improved the content of Ca, Mg, Mn, Cu in the bone, and calcium content increases, show that osteoporosis is in recovery process, body has mobilized more calcium to satisfy the needs that new bone is rebuild, the raising of magnesium, manganese, copper content, the ossein of the bone of then having wholesome effects is synthetic, the stockpiling of alcium and phosphor metabolization and bone ore deposit; Treatment osteoporosis medicinal liquid has also improved femur Trabecula Bone Volume and surface area density and sclerotin volume, illustrates that the mineralization function of bone is tending towards normal; Find also that in addition treatment osteoporosis medicinal liquid also the raise heavy index of testis, spermatogonium number can make the heavy index of testis animal seminal vesicle, the heavy index of prostate raises, and makes normal female rat plasma E
2Level and male rat plasma testosterone level raise, show that this medicine can carry out a part or whole part to hypothalamic pituitary gonadal axis and regulate, because androgen can promote the formation of bone by protein assimilation, and the prescription principal agent Herba Epimedii of treatment osteoporosis medicinal liquid also has the effect of androgen sample, and the rising of gonadal hormone is one of osteoporotic reason of treatment.In the acute toxicity testing to 40 Kunming white mouses, none only shows unusual or dead, gross examination of skeletal muscle is carried out in the white mice dissection, the heart, liver,spleen,kidney, testis, digestive tract etc. organize do not find any unusual, institute's dosage is equivalent to the people and takes 100 times of dose, so can think that treatment osteoporosis medicinal liquid does not have acute toxicity; To active detect have no significant change (P＞0.05) of GPT in the hemogram checking of the SD rat that carries out sub-acute toxicity test, the serum, liver, kidney, testis are carried out conventional section statining microscopy, do not find that all tissue necrosis, inflammation or other are unusual, prove that treatment osteoporosis medicinal liquid has no side effect.Warp is to the trimestral clinical observation of 57 routine senile osteoporosis patients, after taking treatment osteoporosis medicinal liquid, wherein 52 routine lumbar and back pain symptoms obviously alleviate or alleviate, account for 91.23%, and the symptom of suffering from a deficiency of the kidney is obviously improved (P＜0.05, P＜0.001), bone density value has raising (P＜0.05, P＜0.001) in various degree; X-ray shooting vertebra atrophy degree patient does not see that disease further develops, and shows that treatment osteoporosis medicinal liquid can improve the osteoporosis symptom, delays or stops patient's sclerotin further to be lost; Adopt SD-100 type monochromatic light ossiculum ore deposit measuring instrument to measure bone mineral content, find that the patient all has in various degree raising through the treatment bone mineral content, show that treatment osteoporosis medicinal liquid can make bone patient bone amount obtain to a certain degree recovery.It with Herba Epimedii one of the quality index of " bone strengthening refreshing liquid ", stability to treatment osteoporosis medicinal liquid is studied, proof treatment osteoporosis medicinal liquid room temperature is placed a year and a half, no matter be qualitative visual examination or quantitative inherent quality analysis, its quality all is more stable.Clinical effectiveness shows that sufferers of osteoporosis face is after this medicinal liquid treatment, and the lumbar and back pain symptom obviously alleviates or alleviation person accounts for 91.23%.
(4) specific embodiment
Embodiment 1:
Component: 12 parts of Herba Epimedii, 10 parts of the Cortexs Eucommiae, 12 parts of Radix Et Caulis Acanthopanacis Senticosis, 12 parts of Radix Dipsacis, 10 parts of Rhizoma Drynariae, 20 parts of Concha Ostreaes, 8 parts of Carapax et Plastrum Testudiniss, 13 parts of the Radixs Polygoni Multiflori, 14 parts of the Radixs Astragali, 26 parts of Radix Salviae Miltiorrhizaes, 2 parts of Pericarpium Citri Reticulataes, 20 parts in Radix Rehmanniae Preparata is weight portion.Stevioside 0.053 weight portion.
Preparation method:
1, with Herba Epimedii, the Cortex Eucommiae, Radix Et Caulis Acanthopanacis Senticosi, Radix Dipsaci, Rhizoma Drynariae, Concha Ostreae, the Radix Polygoni Multiflori, the Radix Astragali, Radix Salviae Miltiorrhizae, Pericarpium Citri Reticulatae, Radix Rehmanniae Preparata, selected after, take by weighing by the component weight portion, add the water Rapid Cleaning secondary of qdx, soak 1.5 ∽ 2h for the third time.With above-mentioned soaked 11 flavor Chinese medicine decoctings, make heated pressure reach 0.2Mpa to boiling, keep little 1h of boiling (jacketed pan pressure is 0.14 ∽ 0.16Mpa), leach medicinal liquid with 80 purpose stainless (steel) wires, decoct altogether three times, each 1h, amount of water is identical, and three times medicinal liquid merges, and leaves standstill.Get supernatant concentration to 1/3 of amount of liquid medicine, again this concentrated solution is added 95% edible ethanol under fully stirring, reach 30% to alcohol concentration, left standstill precipitate with ethanol 3 hours, pour out supernatant, the residue sediment with link-suspended basket centrifuge 1400r/min centrifugal clear liquid, with this precipitate with ethanol supernatant and centrifugal after clear liquid merge.Clear liquid after merging is squeezed into membrane evaporator, and heating and temperature control is at 87 ℃, and last heating-up temperature rises to 100 ℃, does not have alcohol to medicinal liquid and distinguishes the flavor of, and gets the nothing alcohol concentrated solution of medicinal liquid.The ethanol liquid that reclaims is through the ethanol rectifying column, and rectification to purity is 95%.
2, the Tortoise Shell that takes by weighing is broken into fritter, decocting 3 hours leaches medicinal liquid with 80 purpose stainless (steel) wires, and the nothing alcohol with above-mentioned gained concentrates the medicinal liquid merging again.
3, the Carapax et Plastrum Testudinis granulated slag behind the decocting adds the 2%NaOH and the 0.5%NaCL hydrolysis of 4 times of amounts, shakes 24 hours down at 50 ℃, to the disappearance of granulated slag piece, regulates pH to 8.5 ~ 9, and this medicinal liquid layering gets the transparent supernatant of pale brown color.Add dehydrated alcohol to alcohol concentration and reach 30-90% in this supernatant, room temperature placement 24 hours filters to get filtrate, and this filtrate is boiled off ethanol to there not being the alcohol flavor, gets the Colla Plastri Testudinis of sulfur acid chrondroitin.
4, the medicinal liquid in 2,3 is merged, add 0.053 weight portion stevioside seasoning,, left standstill filter pressing then 4 hours the medicinal liquid mix homogeneously.With Do-30 type filter plate, be cut into the 30cm plectane, overcoat nylon cloth bag installs 19 sheet frames, and with the dual aseptic filtration machine of Ly-0.8 type, pressurization 0.1Mpa can get transparent filtrate,
With 130 ℃ of autoclaving 0.5h of cleaned oral liquid bottle, at the canned medicinal liquid of sterilizing room.Canned back is at 110 ℃ of sterilization 0.5h, leak detection then, pack finished product.
Embodiment 2:
Component: 10 parts of Herba Epimedii, 9 parts of the Cortexs Eucommiae, 10 parts of Radix Et Caulis Acanthopanacis Senticosis, 10 parts of Radix Dipsacis, 9 parts of Rhizoma Drynariae, 18 parts of Concha Ostreaes, 6 parts of Carapax et Plastrum Testudiniss, 12 parts of the Radixs Polygoni Multiflori, 12 parts of the Radixs Astragali, 15 parts of Radix Salviae Miltiorrhizaes, 1 part of Pericarpium Citri Reticulatae, 15 parts in Radix Rehmanniae Preparata is weight portion.Stevioside 0.045 weight portion.
Preparation method is with embodiment 1.
Embodiment 3:
Component: 15 parts of Herba Epimedii, 15 parts of the Cortexs Eucommiae, 15 parts of Radix Et Caulis Acanthopanacis Senticosis, 15 parts of Radix Dipsacis, 15 parts of Rhizoma Drynariae, 24 parts of Concha Ostreaes, 9 parts of Carapax et Plastrum Testudiniss, 15 parts of the Radixs Polygoni Multiflori, 15 parts of the Radixs Astragali, 30 parts of Radix Salviae Miltiorrhizaes, 3 parts of Pericarpium Citri Reticulataes, 24 parts in Radix Rehmanniae Preparata is weight portion.
Preparation method is with embodiment 1.
Embodiment 4:
Component: 13 parts of Herba Epimedii, the Cortex Eucommiae 12,13 parts of Radix Dipsacis, 21 parts of Concha Ostreaes, 14 parts of the Radixs Polygoni Multiflori, 10 parts of Radix Ginsengs, 20 parts of Radix Salviae Miltiorrhizaes, 2 parts of Pericarpium Citri Reticulataes, 20 parts in Radix Rehmanniae Preparata is weight portion.Stevioside 0.05 weight portion.
Preparation method is with the step 1 among the embodiment 1,4.
Embodiment 5: as embodiment 4, different is
Component: 9 parts of Herba Epimedii, the Cortex Eucommiae 9,10 parts of Radix Dipsacis, 19 parts of Concha Ostreaes, 12 parts of the Radixs Polygoni Multiflori, 6 parts of Radix Ginsengs, 16 parts of Radix Salviae Miltiorrhizaes, 1 part of Pericarpium Citri Reticulatae, 16 parts in Radix Rehmanniae Preparata is weight portion.Stevioside 0.035 weight portion.
Embodiment 6: as embodiment 4, different is
Component: 15 parts of Herba Epimedii, the Cortex Eucommiae 14,15 parts of Radix Dipsacis, 23 parts of Concha Ostreaes, 15 parts of the Radixs Polygoni Multiflori, 15 parts of Radix Ginsengs, 30 parts of Radix Salviae Miltiorrhizaes, 3 parts of Pericarpium Citri Reticulataes, 24 parts in Radix Rehmanniae Preparata is weight portion.
Embodiment 7:
Component: 12 parts of Herba Epimedii, 12 parts of the Cortexs Eucommiae, 12 parts of Radix Dipsacis, 20 parts of Concha Ostreaes, 10 parts of Radix Ginsengs, 20 parts of Radix Salviae Miltiorrhizaes, 2 parts of Pericarpium Citri Reticulataes, 20 parts in Radix Rehmanniae Preparata, 10 parts of Rhizoma Drynariae, 8 parts of Carapax et Plastrum Testudiniss, 13 parts of Fructus Lyciis, 13 parts of Radix Angelicae Sinensis.Be weight portion.Stevioside 0.05 weight portion.
Preparation method is with embodiment 1.
Embodiment 8:
Component: 14 parts of Herba Epimedii, 15 parts of the Cortexs Eucommiae, 14 parts of Radix Dipsacis, 22 parts of Concha Ostreaes, 14 parts of Radix Ginsengs, 28 parts of Radix Salviae Miltiorrhizaes, 3 parts of Pericarpium Citri Reticulataes, 24 parts in Radix Rehmanniae Preparata, 12 parts of Rhizoma Drynariae, 9 parts of Carapax et Plastrum Testudiniss, 15 parts of Fructus Lyciis, 15 parts of Radix Angelicae Sinensis.Be weight portion.Stevioside 0.06 weight portion.
Preparation method is with embodiment 1.
Claims (8)
1, treat osteoporotic medicinal liquid, it is characterized in that, be prepared from, be weight portion by following component:
Herba Epimedii 9-15 part, Cortex Eucommiae 9-15 part, Radix Et Caulis Acanthopanacis Senticosi 0-15 part, Radix Dipsaci 10-15 part, Rhizoma Drynariae 0-15 part, Concha Ostreae 18-24 part, Carapax et Plastrum Testudinis 0-9 part, Radix Polygoni Multiflori 0-15 part, Radix Astragali 0-15 part, Radix Salviae Miltiorrhizae 15-30 part, Radix Ginseng 0-15, Pericarpium Citri Reticulatae 1-3 part, Radix Rehmanniae Preparata 15-24 part, Fructus Lycii 0-15, Radix Angelicae Sinensis 0-15.
2, the osteoporotic medicinal liquid of treatment as claimed in claim 1 is characterized in that, is prepared from by following component, is weight portion:
Herba Epimedii 9-15 part, Cortex Eucommiae 9-15 part, Radix Et Caulis Acanthopanacis Senticosi 10-15 part, Radix Dipsaci 10-15 part, Rhizoma Drynariae 9-15 part, Concha Ostreae 18-24 part, Carapax et Plastrum Testudinis 6-9 part, Radix Polygoni Multiflori 12-15 part, Radix Astragali 12-15 part, Radix Salviae Miltiorrhizae 15-30 part, Pericarpium Citri Reticulatae 1-3 part, Radix Rehmanniae Preparata 15-24 part.
3, the osteoporotic medicinal liquid of treatment as claimed in claim 1 is characterized in that, is prepared from by following component, is weight portion:
Herba Epimedii 9-15, Cortex Eucommiae 9-15, Radix Dipsaci 10-15, Concha Ostreae 18-24, Radix Polygoni Multiflori 12-15, Radix Salviae Miltiorrhizae 15-30, Radix Ginseng 6-15, Pericarpium Citri Reticulatae 1-3, Radix Rehmanniae Preparata 15-24.
4, the osteoporotic medicinal liquid of treatment as claimed in claim 1 is characterized in that, is prepared from by following component, is weight portion:
Herba Epimedii 9-15, Cortex Eucommiae 9-15, Radix Dipsaci 10-15, Rhizoma Drynariae 9-12, Carapax et Plastrum Testudinis 6-9, Concha Ostreae 18-24, Radix Ginseng 6-15, Radix Salviae Miltiorrhizae 15-30, Pericarpium Citri Reticulatae 1-3, Radix Rehmanniae Preparata 15-24, Fructus Lycii 12-15, Radix Angelicae Sinensis 12-15.
5, the osteoporotic medicinal liquid of treatment as claimed in claim 1 is characterized in that, also has the flavoring agent of 1/300 weight portion.
6, the osteoporotic medicinal liquid of treatment as claimed in claim 5 is characterized in that, flavoring agent is a stevioside.
7 ,-and the osteoporotic medicinal liquid preparation method of the described treatment of kind of claim 1, comprise the steps:
(1) adopt the technology of water extract-alcohol precipitation to produce the nothing alcohol concentrated solution of Chinese medicine: to take by weighing each component except that Carapax et Plastrum Testudinis by above-mentioned component weight portion, the water Rapid Cleaning secondary that adds qdx, the water logging that adds qdx was for the third time steeped 1.5 ~ 2 hours, decocting is to boiling then, keeping little boiled 0.5-1.5 hour, filter out medicinal liquid, repeat decocting again and fry in shallow oil altogether three times, three times medicinal liquid merges, and leaves standstill, get supernatant concentration to 1/3 of amount of liquid medicine, again this concentrated solution is added 95% edible ethanol under fully stirring, reach 30% to alcohol concentration, left standstill precipitate with ethanol 3 hours, pour out supernatant, the residue sediment centrifugal clear liquid, with the precipitate with ethanol supernatant and centrifugal after clear liquid merge, steaming and decocting is to there not being alcohol again, ethanol reclaims, and must not have alcohol and concentrates medicinal liquid;
(2) take by weighing Carapax et Plastrum Testudinis by above-mentioned component weight portion, be ground into fritter, decocting 2.5-3.5 hour, with filtering out medicinal liquid, the nothing alcohol with above-mentioned gained concentrated the medicinal liquid merging again, and the Carapax et Plastrum Testudinis granulated slag is stand-by;
(3) above-mentioned Carapax et Plastrum Testudinis granulated slag is added the 2%NaOH and the 0.5%NaCL hydrolysis of 4 times of amounts, concussion is 20-25 hour under 50C, disappears to the granulated slag piece, regulates pH to 8.5 ~ 9, this medicinal liquid layering, the transparent supernatant of pale brown color.Add dehydrated alcohol to alcohol concentration and reach 30-90% in this supernatant, placement is 20-25 hour naturally, filters to get filtrate, and this filtrate is boiled off ethanol to there not being the alcohol flavor, gets the Colla Plastri Testudinis of sulfur acid chrondroitin;
(4) Carapax et Plastrum Testudinis decocting liquid and the Colla Plastri Testudinis in the step 3 in the alcohol of the nothing in the step 1 concentrated solution, the step 2 are merged, add 1/300 weight portion stevioside seasoning,, left standstill 3-5 hour the medicinal liquid mix homogeneously, filter pressing then, canned, canned back was 110 ℃ of sterilizations 0.5 hour.
8, the osteoporotic medicinal liquid preparation method of treatment as claimed in claim 7 is characterized in that, filtering liquid medicine uses 80 purpose stainless (steel) wires.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200410023729 CN1284567C (en) | 2004-03-10 | 2004-03-10 | Medicinal liquid for treating osteoporosis and its preparation method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200410023729 CN1284567C (en) | 2004-03-10 | 2004-03-10 | Medicinal liquid for treating osteoporosis and its preparation method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1559589A CN1559589A (en) | 2005-01-05 |
| CN1284567C true CN1284567C (en) | 2006-11-15 |
Family
ID=34441148
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200410023729 Expired - Fee Related CN1284567C (en) | 2004-03-10 | 2004-03-10 | Medicinal liquid for treating osteoporosis and its preparation method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1284567C (en) |
Families Citing this family (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100418553C (en) * | 2005-12-09 | 2008-09-17 | 康辰医药股份有限公司 | Preparation for treating osteoporosis and preparation method thereof |
| CN101069716B (en) * | 2006-05-10 | 2011-02-16 | 河北天时医药技术开发有限公司 | Chinese medicine composition for treating osteoporous and preparing method |
| CN101129448B (en) * | 2006-08-21 | 2012-04-25 | 兰州大得利生物化学制药(厂)有限公司 | Compositions for preventing and controlling climacteric syndrome and osteoporosis, and processes for producing the same |
| US9101161B2 (en) * | 2006-11-02 | 2015-08-11 | The Coca-Cola Company | High-potency sweetener composition with phytoestrogen and compositions sweetened therewith |
| CN101468104B (en) * | 2007-12-24 | 2011-09-21 | 上海中医药大学附属曙光医院 | Chinese medicinal compound preparation for treating osteoporosis and method for preparing the same |
| CN101292739B (en) * | 2008-05-21 | 2012-02-01 | 无限极（中国）有限公司 | Health food having function of increasing bone substance density |
| CN102233028B (en) * | 2011-05-20 | 2012-07-25 | 魏玉玲 | Composite preparation for treating osteoporosis |
| CN103550476B (en) * | 2013-11-13 | 2015-09-16 | 崔合芳 | A kind of pharmaceutical composition for the treatment of postmenopausal osteoporosis and preparation method thereof |
| CN104027476A (en) * | 2014-07-01 | 2014-09-10 | 华梅 | Traditional Chinese medicine white spirit for treating osteoporosis and preparation method thereof |
| CN104606619B (en) * | 2015-02-03 | 2017-08-29 | 山东省立医院 | A kind of medicine for treating osteoporosis |
| CN104688932A (en) * | 2015-04-08 | 2015-06-10 | 房经武 | Traditional Chinese medicine preparation for postmenopausal osteoporosis and preparation method |
| CN105833178A (en) * | 2016-04-26 | 2016-08-10 | 于龙 | Traditional Chinese medicine composition for treating bone injuries and bone diseases |
| CN106938005A (en) * | 2017-05-03 | 2017-07-11 | 辽宁中医药大学 | A kind of Chinese medicine composition for being used to treat osteoporosis |
-
2004
- 2004-03-10 CN CN 200410023729 patent/CN1284567C/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| CN1559589A (en) | 2005-01-05 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN1150019C (en) | Medicine for treating hecrosis of femoral head | |
| CN101513437B (en) | Antifatigue Chinese pharmaceutical composition and preparation method thereof | |
| CN1284567C (en) | Medicinal liquid for treating osteoporosis and its preparation method | |
| CN1139396C (en) | Life essence-filling, marrow-benefitting and kidney invigorating Chinese medicine preparation | |
| CN101062080B (en) | Medicinal composition for relieving fatigue and preparing process thereof | |
| CN1899479A (en) | Chinese medicine compound preparation for treating osteoporosis after menopause and its preparation | |
| CN101053621A (en) | Traditional Chinese medicinal composition for treating obesity and its preparation method | |
| CN107349333A (en) | Composition with increase bone density effect and its preparation method and application | |
| CN101095768B (en) | Chinese traditional medicine capsule for treating primary hypertension for persons in middle and old age | |
| CN102671178B (en) | Preparation method of Chinese medicinal composition for treating nephrotic syndrome and uremia and application thereof | |
| CN102274481A (en) | Chinese medicinal preparation for treating rheumatoid arthritis | |
| CN100418553C (en) | Preparation for treating osteoporosis and preparation method thereof | |
| CN101138597B (en) | Traditional Chinese medicine preparation for preventing and controlling osteoporosis and method of preparing the same | |
| CN1899590A (en) | Gynecological Chinese patented medicine for relieving depressed liver and clearing heat, strengthening spleen and nourishing blood and its preparing method | |
| CN102293923B (en) | Application of Tibetan medicinal composition to preparation of medicament for preventing and treating osteoporosis | |
| CN105434797A (en) | Bone nourishing and body building oral liquid and preparation method thereof | |
| CN101293069B (en) | Traditional Chinese medicine for treating sub-health | |
| CN1170553C (en) | Medicinal wine for treating rheumatic and rheumatoid diseases and its preparing prcess | |
| CN1562116A (en) | Chinese medicine preparation with invigorating kidney function | |
| CN1061846C (en) | Traditional Chinese medicine prescription for tonifying the kidney reinforcing the spleen and nourishing the blood and preparation process | |
| CN1238021C (en) | Medicine for treating osteoproliferation | |
| CN106860656A (en) | It is a kind of to treat Chinese medicine composition of osteoporosis and its preparation method and application | |
| CN101843668A (en) | Chinese medicinal granules for treating femoral head necrosis and preparation method thereof | |
| CN105012507A (en) | Traditional Chinese medicine composition for treating osteoporosis and preparation method of traditional Chinese medicine composition | |
| CN105535161A (en) | Traditional Chinese medicine for treating osteoporosis and preparation method of traditional Chinese medicine |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C19 | Lapse of patent right due to non-payment of the annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |