CN1279182C - 通用多变异体检测系统 - Google Patents

通用多变异体检测系统 Download PDF

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Publication number
CN1279182C
CN1279182C CNB028102681A CN02810268A CN1279182C CN 1279182 C CN1279182 C CN 1279182C CN B028102681 A CNB028102681 A CN B028102681A CN 02810268 A CN02810268 A CN 02810268A CN 1279182 C CN1279182 C CN 1279182C
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Prior art keywords
nucleic acid
primer
downstream primer
probe
acid sequence
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Expired - Fee Related
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CNB028102681A
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English (en)
Chinese (zh)
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CN1509338A (zh
Inventor
琳达·安德勒斯
卡门·妮古拉·尼科尔斯
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New York Blood Center Inc
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New York Blood Center Inc
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C12Q1/706—Specific hybridization probes for hepatitis
    • C12Q1/707—Specific hybridization probes for hepatitis non-A, non-B Hepatitis, excluding hepatitis D
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
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    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C12Q1/702—Specific hybridization probes for retroviruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C12Q1/702—Specific hybridization probes for retroviruses
    • C12Q1/703—Viruses associated with AIDS
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C12Q1/706—Specific hybridization probes for hepatitis

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Virology (AREA)
  • Communicable Diseases (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • AIDS & HIV (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Measurement Of Velocity Or Position Using Acoustic Or Ultrasonic Waves (AREA)
  • Small-Scale Networks (AREA)
CNB028102681A 2001-04-17 2002-04-17 通用多变异体检测系统 Expired - Fee Related CN1279182C (zh)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US28433401P 2001-04-17 2001-04-17
US60/284,334 2001-04-17

Publications (2)

Publication Number Publication Date
CN1509338A CN1509338A (zh) 2004-06-30
CN1279182C true CN1279182C (zh) 2006-10-11

Family

ID=23089805

Family Applications (1)

Application Number Title Priority Date Filing Date
CNB028102681A Expired - Fee Related CN1279182C (zh) 2001-04-17 2002-04-17 通用多变异体检测系统

Country Status (12)

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US (4) US7348164B2 (enExample)
EP (1) EP1390544B1 (enExample)
JP (1) JP4271944B2 (enExample)
KR (1) KR20040018354A (enExample)
CN (1) CN1279182C (enExample)
AT (1) ATE346958T1 (enExample)
AU (1) AU2002307359B2 (enExample)
CA (1) CA2444174A1 (enExample)
DE (1) DE60216445T2 (enExample)
ES (1) ES2278043T3 (enExample)
WO (1) WO2002083927A2 (enExample)
ZA (1) ZA200308906B (enExample)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2002307359B2 (en) * 2001-04-17 2007-09-13 The New York Blood Center, Inc. Universal multi-variant detection system
EP2251441B1 (en) * 2004-02-10 2013-05-08 F. Hoffmann-La Roche AG Detection of parvovirus B19
AU2011253599B2 (en) * 2004-09-30 2013-12-12 Gen-Probe Incorporated Assay for detecting and quantifying HIV-1
CA2929741A1 (en) 2004-09-30 2006-04-13 Gen-Probe Incorporated Assay for detecting and quantifying hiv-1
US7833716B2 (en) * 2006-06-06 2010-11-16 Gen-Probe Incorporated Tagged oligonucleotides and their use in nucleic acid amplification methods
WO2008080029A2 (en) 2006-12-21 2008-07-03 Gen-Probe Incorporated Methods and compositions for nucleic acid amplification
WO2010105275A2 (en) 2009-03-13 2010-09-16 Cornell University Method to assess human allograft status from microrna expression levels
EP2449132B1 (en) 2009-07-01 2015-05-13 Gen-Probe Incorporated Methods and compositions for nucleic acid amplification
US9746479B2 (en) 2010-03-09 2017-08-29 Cornell University Methods and compositions to predict and detect acute rejection
WO2014018885A2 (en) 2012-07-27 2014-01-30 Gen-Probe Incorporated Dual reference calibration method and system for quantifying polynucleotides
KR101377824B1 (ko) * 2012-11-19 2014-04-01 가톨릭대학교 산학협력단 수인성 바이러스 검출용 프라이머 및 이를 포함하는 검출 키트
CN103901000B (zh) * 2012-12-26 2016-09-28 江南大学 一种基于荧光共振能量转移检测伏马菌毒素b1的方法
US9758828B2 (en) 2013-01-31 2017-09-12 Cornell University Methods to detect, treat and prevent acute cellular rejection in kidney allografts
US9982301B2 (en) 2014-01-07 2018-05-29 Cornell University Urine mRNA profile and acute dysfunction of kidney allograft
EP4560026A3 (en) 2014-04-10 2025-08-27 The Trustees of the University of Pennsylvania Use of micro-ribonucleic acid (mirna) to diagnose transplant rejection and tolerance of immunosuppression therapy
US11339436B2 (en) 2015-02-03 2022-05-24 The Trustees Of The University Of Pennsylvania Methods for early identification of bone healing ability in injured patients
US10370731B2 (en) 2016-06-10 2019-08-06 Roche Molecular Systems, Inc. Compositions and methods for detection of hepatitis C virus genotype 3
CN110699353A (zh) * 2019-07-26 2020-01-17 安徽大学 一种靶向端粒的核酸探针
CN120265769A (zh) * 2023-10-08 2025-07-04 迈克生物股份有限公司 引物探针组合物、核酸检测方法及应用

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5925517A (en) * 1993-11-12 1999-07-20 The Public Health Research Institute Of The City Of New York, Inc. Detectably labeled dual conformation oligonucleotide probes, assays and kits
US6007983A (en) * 1995-12-22 1999-12-28 Visible Genetics Inc. Method and kit for evaluation of HIV mutations
DE69738687D1 (de) * 1996-04-12 2008-06-26 Phri Properties Inc Sonden, kits und assays
US6117635A (en) 1996-07-16 2000-09-12 Intergen Company Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon
US6037130A (en) * 1998-07-28 2000-03-14 The Public Health Institute Of The City Of New York, Inc. Wavelength-shifting probes and primers and their use in assays and kits
AU3438999A (en) 1999-05-07 2000-11-21 Nationales Zentrum Fur Retroviren Detection system for human immunodeficiency virus based on nucleic acid amplification
US6759193B2 (en) * 1999-07-28 2004-07-06 The Government Of The Republic Of Singapore Detection of human hepatitis B virus surface antigen mutants by specific amplification and its application on gene chip
AU2002307359B2 (en) * 2001-04-17 2007-09-13 The New York Blood Center, Inc. Universal multi-variant detection system

Also Published As

Publication number Publication date
US7348164B2 (en) 2008-03-25
ZA200308906B (en) 2004-11-23
CN1509338A (zh) 2004-06-30
JP2004532027A (ja) 2004-10-21
ATE346958T1 (de) 2006-12-15
US20120258446A1 (en) 2012-10-11
ES2278043T3 (es) 2007-08-01
US8192939B2 (en) 2012-06-05
EP1390544A2 (en) 2004-02-25
CA2444174A1 (en) 2002-10-24
US20130288231A1 (en) 2013-10-31
US20110053138A1 (en) 2011-03-03
JP4271944B2 (ja) 2009-06-03
DE60216445T2 (de) 2007-10-31
DE60216445D1 (de) 2007-01-11
EP1390544A4 (en) 2004-07-14
HK1065825A1 (zh) 2005-03-04
EP1390544B1 (en) 2006-11-29
AU2002307359B2 (en) 2007-09-13
KR20040018354A (ko) 2004-03-03
WO2002083927A3 (en) 2003-03-20
US20040053284A1 (en) 2004-03-18
WO2002083927A2 (en) 2002-10-24

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