CN1277912C - Process of extracting glasswort oil from glasswort plant - Google Patents
Process of extracting glasswort oil from glasswort plant Download PDFInfo
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- CN1277912C CN1277912C CN 200410060758 CN200410060758A CN1277912C CN 1277912 C CN1277912 C CN 1277912C CN 200410060758 CN200410060758 CN 200410060758 CN 200410060758 A CN200410060758 A CN 200410060758A CN 1277912 C CN1277912 C CN 1277912C
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- oil
- glasswort
- linolic acid
- froggrass
- mixing
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Abstract
The present invention relates to a method for extracting froggrass oil (active substance) from natural froggrass plants. The complete froggrass plants and seeds which are dried and pulverized or milled into pulp are added in water to be stirred, heated and steamed. When the mixture is cooled from 40 to 60 DEG C., an enzyme preparation is added to regulate the pH value from 2 to 10 to carry out enzymolysis for 1 to 20 hours, heating and deposit filtration, and an enzymolysis liquid is obtained to extract froggrass mixing oil by an organic solvent; or a linoleic acid in the froggrass mixing oil is converted into conjugated linoleic acid by linoleate isomerase. The mixing oil extraction rate of the method is as high as 29 to 35%, and the linoleic acid ratio in the froggrass oil is more than or equal to 74%.
Description
Technical field:
The present invention relates to a kind of method of extracting natural active matter from natural phant, specifically is the method for extracting glasswort oil from the fluffy plant in sea.
Background technology:
Hai Peng (Salicornia bigclvii Torr) originates in the beach and landlocked sabkha of temperate zone, North America and subtropical zone, is a kind of no leaf, Carnified annual sabkha plant, and seed production is very high.The fluffy whole body in sea is precious, and it is edible that its young stem and leaf can be done vegetables, and its seed oil content is higher than 35%.Linolic acid contains 74% in the Salicornia Bigelovii Torr. mixing oil, linolenic acid contains 2.6%, wherein linolic acid is that the necessary and human body self of HUMAN HEALTH growth can not synthetic lipid acid, it has nourishing brain cell, regulate the effect of vegetative nerve function, synthetic and other physiological activity of the activity of enzyme, prostaglandin(PG) in the contraction of participation human smooth muscle, the lipid metabolism.Clinically be used to adjust blood cholesterol, arteriosclerotic treatment and prevention.And linoleic isomer conjugated linolic acid then has antitumor, reducing cholesterol and triglyceride level and low density lipoprotein cholesterol, atherosclerosis, raising immunizing power, improves skeleton density, prevents and treats diabetes and can reduce animal and human's body fat, increases effects such as muscle.
At present internal and international going up all adopted mechanical oil expression method to the extraction of glasswort oil, and adopts chemical process to produce conjugated linolic acid.This method is not high to the limited glasswort oil extraction yield that makes of vegetable cell destructiveness because of mechanical processes, only reach 21%～24%, linolic acid is isomerizated into conjugated linolic acid, transformation efficiency also only reaches 50%～65%, and can make the sex change aggravation of linolic acid in the oil plant and conjugated linolic acid etc. because of produce heating power processing that conjugated linolic acid exists with chemical process, influence their further utilization.Because in vegetable oil material, grease is present in the cell of vegetable oil material, and (protein and carbohydrate matter combine existence, constitute complex bodys such as lipoprotein, lipopolysaccharides with other macromole usually, have only cellularstructure and grease compounded body to destroy, just can extract grease oil plant.
Summary of the invention:
The objective of the invention is to overcome the defective that traditional mechanical oil expression method exists, adopt aqueous enzymatic extraction glasswort oil technology, both reduced extraction cost, improved oil yield, can guarantee the linoleic content of effective ingredient again, and make conjugated linolic acid be able to efficient conversion.
Aqueous enzymatic extraction technology of the present invention, be to get the extra large fluffy plant complete stool of dry and pulverizing or defibrination or the water that seed adds 1～10 times of raw material weight, after fully stirring, soak, hot digestion 1～4 hour, put then and be chilled to 65 ℃～40 ℃, adding zymin fully stirs, regulate pH value to 2～10, through enzymolysis after 1～20 hour, be heated 80 ℃～100 ℃, heavy through filter in 20～50 minutes, get enzymolysis solution and get the Salicornia Bigelovii Torr. mixing oil with organic solvent extraction, or again glasswort oil is further extracted linolic acid, with linoleate isomerase linolic acid is converted into conjugated linolic acid again.
When the present invention adopted linoleate isomerase to transform linolic acid in linolic acid or the glasswort oil, the consumption of linoleate isomerase was 0.5%～1%, regulates pH value 6.5～7.5, cultivates 35～48 hours under 30 ℃～40 ℃ conditions.
Zymin of the present invention comprises amylase, proteolytic enzyme, cellulase, hemicellulase, polygalacturonase, and they can be used singly or in combination.
Adopt the aqueous enzymatic extraction glasswort oil, extraction yield contains linoleic ratio 〉=74% up to 29%～35% in institute's extract oil; With microbial enzyme the transformation efficiency that linolic acid is converted into conjugated linolic acid is reached 80%～90%.Compare than traditional method, the extraction yield of glasswort oil and the transformation efficiency of conjugated linolic acid all are greatly improved.
The glasswort oil that the inventive method is extracted can be made into health-care preparations such as dripping pill, soft capsule, also it can be joined and make makeup in the cosmetic material, it can also be added in the various food and make protective foods.
Description of drawings:
Fig. 1 is a process flow sheet of the present invention.
Embodiment:
Embodiment 1:
Get dry Salicornia Bigelovii Torr. plant, pulverize the water that the back adds 3 times of raw material weights, fully stirred the post-heating boiling 1 hour, put when being chilled to 50 ℃, add the proteolytic enzyme of commercial weight weight 0.5%, regulate pH value 5.0, maintained the temperature under 55 ℃ of conditions enzymolysis then 5 hours.Be heated heavy 30 minutes of 100 ℃ of filters behind the enzymolysis, get enzymolysis solution and add Petroleum ether extraction, after reclaiming sherwood oil, get glasswort oil.
Embodiment 2:
Get 2 kilograms in dry Salicornia Bigelovii Torr. seed, add 5 kilograms in water, fully stirred the post-heating boiling 2 hours, add 5 gram proteolytic enzyme, 5 gram cellulases, 5 gram polygalacturonases when being cooled to 60 ℃, regulate pH value 5.5, maintained the temperature under 60 ℃ of conditions enzymolysis then 6 hours.Be heated 90 ℃ of filtering-depositings 45 minutes behind the enzymolysis, get enzymolysis solution and add methanol extraction, after reclaiming methyl alcohol, get glasswort oil.
Embodiment 3:
Get 1 kilogram of glasswort oil, add 10 gram linoleate isomerases (Lactobacillus Yeuferi PYR8 (Atcc 55739)), regulate pH value 7, under 35～37 ℃ of conditions, cultivated 42 hours, the linolic acid in the glasswort oil can be converted into conjugated linolic acid.
Embodiment 4:
Taking polyethylene glycol 800 grams, heating and melting adds glasswort oil 200 grams and stirs evenly, and splashes into whiteruss pipeline (drilling pill device), leaches dripping pill, promptly gets the glasswort oil dripping pill.
Embodiment 5:
Get glasswort oil 25 grams, gelatin 50 grams, glycerine 25 grams.With the molding of gelatin glycerol adding heating and melting, inject glasswort oil by capsule forming machine, the glasswort oil soft capsule is made in compression molding.
Embodiment 6:
Take by weighing component in following ratio: glasswort oil 19%, linolic acid 20%, glyceryl monostearate 10%, hexadecanol 11%, triple pressed stearic acid 10%, XU 61518.10 5%, spermaceti 7%, Vaseline 8%, the husky alkene 5% in angle, cholesteryl ester 5%.Above-mentioned each component stirred make the paste body shape makeup.
Embodiment 7:
Get 1.5 parts of glasswort oils, 6 parts in gelatin, 6 parts of gum arabics, 0.5 part of acroleic acid resin.Gelatin and gum arabic are fused in 40 ℃ of hot water, add glasswort oil, in dispersing emulsification machine, form emulsion, when checking that the elaioleucite diameter is 6～10 μ, add acroleic acid resin, in 210 parts of hot water, stir, and transfer pH value to 0.5, add 2.5 parts of 37% formaldehyde solutions again with 5% acetic acid, wait to restraint the film sclerosis, transfer pH value to 10 with sodium hydroxide in addition, solution is in 50 ℃ of heating 20 minutes, the centrifugal 25 μ micro-capsules that obtain.The glasswort oil micro-capsule is added in various soft material or the fruit juice can obtains muddy shape beverage or fruit juice.
Embodiment 8:
Get 50 parts in flour, 28 parts of glasswort oils, 0.15 part of salt, 20 parts of white sugar, 0.2 part in bicarbonate of ammonia, 0.3 part in sodium bicarbonate, dairy products are an amount of, and water is an amount of, 0.08 part of spices.With raw material modulation, rolling, the crush-cutting moulding is after baking, cooling, arrangement promptly get the glasswort oil biscuit.
Claims (2)
1, a kind of from the sea method of fluffy plant extract glasswort oil, it is characterized in that getting the extra large fluffy plant complete stool of dry and pulverizing or defibrination or the water that seed adds 1～10 times of raw material weight, after fully stirring, soak, hot digestion 1～4 hour, put then and be chilled to 40 ℃～60 ℃, add amylase, proteolytic enzyme, cellulase, hemicellulase, a kind of or some kinds of mixing in the polygalacturonase are used, fully stir, regulate pH value to 2～10, through enzymolysis after 1～20 hour, be heated 80 ℃～100 ℃, heavy through filter in 20～50 minutes, get enzymolysis solution and get the Salicornia Bigelovii Torr. mixing oil with sherwood oil or methanol extraction; Or with linoleate isomerase the linolic acid in the Salicornia Bigelovii Torr. mixing oil is converted into conjugated linolic acid again.
2, according to claim 1 described from the sea method of fluffy plant extract glasswort oil, the adding weight ratio that it is characterized in that linoleate isomerase is 0.5%～1.0%, regulate pH value to 6.5～7.5 then, under 30 ℃～40 ℃ conditions, cultivate and made linolic acid be converted into conjugated linolic acid in 35～48 hours.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200410060758 CN1277912C (en) | 2004-08-20 | 2004-08-20 | Process of extracting glasswort oil from glasswort plant |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200410060758 CN1277912C (en) | 2004-08-20 | 2004-08-20 | Process of extracting glasswort oil from glasswort plant |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1597891A CN1597891A (en) | 2005-03-23 |
| CN1277912C true CN1277912C (en) | 2006-10-04 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200410060758 Expired - Fee Related CN1277912C (en) | 2004-08-20 | 2004-08-20 | Process of extracting glasswort oil from glasswort plant |
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| CN (1) | CN1277912C (en) |
Families Citing this family (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1331424C (en) * | 2005-10-26 | 2007-08-15 | 张春银 | Freeze dried crab grass and method for preparing deli of rest and recreation of crab grass |
| CN100341431C (en) * | 2005-10-26 | 2007-10-10 | 张春银 | Powder and grains of crab grass, and preparation method |
| CN1328975C (en) * | 2005-10-26 | 2007-08-01 | 张春银 | Fine dried noodles, dragon beard noodles or instant noodles of crab grass, and preparation method |
| CN101438740B (en) * | 2007-11-21 | 2013-06-05 | 上海市粮食科学研究所 | Technique for producing lipid containing natural conjugate linoleate |
| CN101953764B (en) * | 2010-09-15 | 2012-01-04 | 盐城工学院 | Application of samphire extract in inhibiting tyrosinase activity |
| CN102181321B (en) * | 2011-04-06 | 2013-02-27 | 贵州大学 | Method for preparing oil by low aqueous enzymatic method and organic solvent extraction |
| CN102181319B (en) * | 2011-04-06 | 2013-02-13 | 贵州大学 | Oil making method based on lower-moisture enzymatic auxiliary cold pressing |
| CN104152260A (en) * | 2014-09-08 | 2014-11-19 | 南京飞马食品有限公司 | Biological extraction method of high-unsaturated-activity salicornia bigelovii seed oil |
| CN105154213A (en) * | 2015-07-23 | 2015-12-16 | 华侨大学 | Extraction method for tea seed oil containing conjugated linoleic acid |
| CN105542937A (en) * | 2015-12-08 | 2016-05-04 | 李尚锋 | Method for extracting grease from soybeans through deep processing |
| CN106578140A (en) * | 2016-12-13 | 2017-04-26 | 青岛嘉瑞生物技术有限公司 | Healthcare Salicornia Bigelovii Torr. and sesame seed oil |
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2004
- 2004-08-20 CN CN 200410060758 patent/CN1277912C/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
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| CN1597891A (en) | 2005-03-23 |
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