KR101099197B1 - Anticancerous fermented oil composition - Google Patents

Abstract

The present invention relates to an anticancer fermented oil and fat composition obtained by fermenting chlorosis and lychee fruit by a plant enzyme method in order to produce a composition for inhibiting cancer cell growth. According to the present invention, it can be filled with soft capsules in order to be more convenient to intake than conventional methods for producing health benefits of medicines and health foods by traditional methods, quantifying a certain number of capsules according to the condition of cancer patients As well as can be prepared, cancer patients have an effect that can be easily ingested.

Anticancer, fermentation, edible oil, lychee fruit, white flower

Description

Production method of anticancer composition by oil phase fermentation {ANTICANCEROUS FERMENTED OIL COMPOSITION}

The present invention relates to a method for producing an anticancer composition by oil phase fermentation.

The increasing number of cancer diseases, which poses the greatest danger to modern man and is a social issue, is becoming more serious due to environmental pollution. Sixty to seventy percent of deaths from natural deaths or diseases have been confirmed to have been caused by various cancers, and fearful results have been announced that one in four people per cancer are cancer patients.

Therefore, modern medicine is making scientific attempts to solve this problem, and methods of treatment through natural medicines are constantly being studied.

When explaining the outline and research process of the Journey fruit and baekhwacho that are known to be effective in cancer among the natural drugs are as follows.

The journey tree refers to the ash tree of Ligustrum japonicum or Ligustrum obtusifolium, which is native to the southern coast of Korea, Jeju Island, Japan, Taiwan and China. Its fruit, Journeysil, has traditionally been used as a herbal medicine and is known to be used for hemostasis, health, tonic, cold, earth blood, bloody stools, and fatigue recovery. According to the recent literature, it is effective in obesity, constipation, cerebral nervous system diseases (stroke, dementia), inflammation, hair growth, etc., and antioxidative activity, antiviral activity, hepatoprotective effect, anticancer activity and the like have been reported.

On the other hand, Oledenlandia diffusa (Oldenlandia diffusa), also known as twin fishing grass, is named for its flower color and leaf shape resembling the tongue of a snake. And Baekhwasaengcho grows in the wetlands of the mountain, 10-30cm in height, branches branched from the bottom to grow laterally or stand straight. Or leaves are opposite, lined, without sawtooth, but edge is clear and only vein is appeared, 1 ~ 3.5cm long and 1.5 ~ 3mm wide.

Recently, the results of various papers have been reported to be very effective anti-cancer effect of baekhwasadancho. In other words, the baekhwasajeong is effective in stomach cancer, liver cancer, esophageal cancer, rectal cancer, bladder cancer. It is also very effective in taking tumors and inflammation, which is good for the digestive and lymphatic tumors.

In the manufacture and processing to treat humans or health benefits using baekryeoksaeng and lychee fruit, such as the traditional method is to mix the mixed powder, raw materials to produce a ring, or distilled liquor by dipping in alcohol such as shochu Made or consumed.

However, filling traditional capsules with soft capsules for easy intake was not easy with traditional methods. Raw materials and finished products obtained by the traditional method are fermentation liquid obtained by fermentation by adding alcohol (water soluble), powder, CO ₂ extraction solution (water soluble), alcohol leaching liquor (alcohol and water soluble to promote soft capsule disintegration), and sugar. Water-soluble), etc., even if it is possible to fill in a soft capsule, there was a problem that it is not possible to preserve it because it disintegrates within 1 hour. This is because the raw material filled in the soft capsule should be a raw material processed with fat-soluble fat or oil, but the water-soluble raw material cannot be filled with the soft capsule.

Therefore, if the final product is a water-soluble liquid that is not an oil-based ingredient, it cannot be filled into the soft capsule.If it is a concentrated liquid, it can be filled with the soft capsule.However, it is difficult to store it after filling.If it is stored for a long time, the concentrated liquid inside the filled capsule solidifies and decomposes after ingestion. Filling the capsules of the concentrate is not an effective method. In particular, people with serious illnesses, such as cancer patients, should not be hardened so that the disintegration rate may be faster or absorption may be accelerated after ingestion.

In other words, it is not only impossible and not attempted to prepare Journey fruit and chlorosis composition with soft capsules by conventional methods, and the final product must be a fat-soluble fat or oil component to produce soft capsules. By the production process, the oil-fat component which contained the journey room component and the whitening sand component could not be obtained.

In addition, the fruit of the Journey is like a normal seed with a soft skin on the outer skin, and the inner skin is wrapped in a hard skin and contains a large amount of protein and fat in the skin, so fermentation was not easy.

In addition, it is not clear whether the lychee fruit and baekryoksaul composition according to the conventional manufacturing method is effective for anticancer when prepared by any method. Therefore, although it is known to be effective in anti-cancer, the wide utilization of the fruit of the Journeysil and Pear vulgaris has not been achieved.

Therefore, the present invention was devised in view of the above-mentioned conventional problems, and an object of the present invention is to provide a soft capsule in order to be conveniently ingested than the method for preparing the beneficial medicines and health foods by the conventional method. It is to provide an anticancer fermented fat and oil composition that can be filled with.

Another object of the present invention is to provide an anti-cancer fermentation and maintenance composition that is filled with soft capsules, which is easy to preserve and convenient to ingest, and has an excellent effect on inhibiting the growth of anticancer and cancer cells.

According to a feature of the present invention for achieving the object as described above, the first invention relates to a method for producing an anticancer composition by oil phase fermentation, 1 to 5% by weight of alcohol alcohol mixed with the yeast fruit Aging for 20 to 30 hours; Injecting a first mixture of 25-65% by weight of vegetable edible oil into the fermented fruit and ethanol relative to the fermented fruit and alcohol; Fermenting the first mixture for 30 to 60 days at an internal temperature of 20 to 38 ° C .; Compressing the fermented first mixture to extract a first fermented fat and oil composition; Injecting a second mixture of baekhwasa vinegar dried at 20 to 35% of water content with a vegetable edible oil of the same weight as baekhwasacho vinegar to the fermentor; Fermenting the second mixture for 30 to 60 days at an internal temperature of 20 to 38 ° C .; Compressing the fermented second mixture to extract a second fermented fat and oil composition; And mixing the first fermented fat and oil composition and the second fermented fat and oil composition.

The second invention relates to an anti-cancer fermented fat and oil composition, the step of ripening 20 to 30 hours by mixing 1 to 5% by weight of alcohol with respect to the fermented fruit and the fermented fruit; Whitening hydrangea dried with 20 to 35% water content of the same weight as the journey fruit, and 25 to 65% by weight of vegetable edible oil with respect to the journey fruit and alcohol and ethanol; Introducing the mixture into the fermentor; Fermenting the third mixture for 30 to 60 days using an internal temperature of the fermenter at 20 to 38 ° C; And extracting the third fermented fat and oil composition by pressing the fermented third mixture.

In the third invention, in the first or second invention, the frequency of the alcohol is characterized in that 15 to 25 degrees.

The fourth invention is characterized in that in the first or second invention, the fermenter is a vacuum emulsifier capable of fermentation in a vacuum state.

The fifth invention, in the fourth invention, in the fermentation step, characterized in that the first to third mixtures are stirred for 3 to 5 hours daily at 40 to 100 times per minute.

The sixth invention is characterized in that, in the first or second invention, the vegetable edible oil is one of grape seed oil, soybean oil, jade oil, olive oil, pumpkin seed oil, sunflower seed oil, flaxseed oil, and rapeseed oil.

According to the present invention obtained through the above object and the technical solution for achieving the object, it can be filled with soft capsules to be more convenient to ingest than the method for preparing the medicine and health foods beneficial to health by the traditional method According to the cancer patient, not only can a certain number of capsules be prepared in a quantitative manner, but also has an effect that cancer patients can easily consume.

In addition, according to the present invention, it is filled with a soft capsule can be easily preserved and convenient to consume, but can exert an excellent effect in inhibiting the growth of anti-cancer and various cancer cells, there is an advantage that can be widely used in various types of cancer treatment.

Hereinafter, the present invention will be described in detail with reference to the accompanying drawings.

First Embodiment

1 is a flow chart showing a first embodiment of the anticancer fermentation fat and oil composition according to the present invention.

As shown, in the present embodiment, fermented fat and oil compositions are fermented separately after fermentation of fermented fruit and baekrye saengcho, and the anti-fermented fat and oil composition is made by mixing the fermented fat and oil composition. In particular, the fermentation process according to this embodiment is made by a plant enzyme method.

Here, the phytoenzyme method means a process in which the activity of the enzyme is made in the anoxic state and the medium is fermented. For example, when a person ingests food, he or she digests food along with various breakdown liquids that break down the food ingested. In this process, organic matter is produced by enzymes, and organic matter is absorbed into the human body to obtain nutrients. It is similar to fermentation process by plant enzyme method.

The manufacturing method according to the present embodiment will be described in more detail. First, in order to ferment the lychee fruit, the lychee fruit is first harvested, washed to remove impurities, and then pulverized by feeding into a crusher. The journey fruit is then crushed so that the hard coat in the pulp is pulverized and the ovary and pulp are mixed to form a slight resemblance.

Thereafter, 1 to 5% by weight of alcohol alcohol is mixed with the fruit of the journey and the fruit of the journey, aged for about 20-30 hours. At this time, it is preferable that the temperature of aging is approximately 30 ° C, and the frequency of the alcohol is preferably in the range of 15 to 25 (step S10).

25 to 65% by weight of vegetable edible oil is mixed with the fermented fruit and the alcohol to produce the first mixture and fermented to the fermenter. The fermenter used at this time is preferably a vacuum emulsifier capable of fermentation in a vacuum state.

In other words, the composition to be obtained in the present invention is immersed in the edible oil with a solvent of the lyosil fruit and the lyotropic fruit is fermented for a long time by the plant enzyme method. This process can be considered in the use of fermenters and in duga fat using a fully enclosed vacuum emulsifier. However, since the fermenter is likely to introduce some oxygen during the fermentation process, it is more preferable to use a vacuum emulsifier to effectively control the temperature by blocking the inflow of oxygen during the fermentation period.

And the vegetable edible oil is used by selecting one of grape seed oil, soybean oil, jade oil, olive oil, pumpkin seed oil, sunflower seed oil, flaxseed oil, rapeseed oil. In particular, among the vegetable edible oils and fats, it is more preferable to use flaxseed oil and grapeseed oil which are expected to be effective against cancer (step S20).

In the vacuum emulsifier, the first mixture is fermented for 30 to 60 days while stirring at an internal temperature of 20 to 38 ° C. for 3 to 5 hours daily at 40 to 100 times per minute while maintaining a vacuum. At this time, the stirring is to prevent the first mixture from being hardly agglomerated in the vegetable edible oil, and if it is not stirred for one day, the first mixture is lowered down the edible oil and fat. Therefore, in order to effect the fermentation is to stir as described above (step S30).

After the fermentation is completed, the first mixture is compressed to extract a fermentation fat and oil composition (hereinafter referred to as "first fermentation fat and oil composition") (step S40).

Next, fermented white birch.

In other words, white chlorosis is harvested and washed, foreign matter and impurities are removed, and dried to 20 ~ 35% moisture content. Then, the dried white chalcedony is cut into 3 to 15 cm in size, and the second mixture mixed with 50 to 200% by weight of vegetable edible oil is added to the fermenter. The fermenter at this time also uses a vacuum emulsifier.

On the other hand, in the case of using completely dried hay bleached hay vinegar, the hay and raw grass may be mixed in a 1: 1 ratio. Of course, even when using a white baeksasulcho with a moisture content of 20-35% may be used by mixing the raw vinegar in the same ratio. At this time, the fermentation of baekhwasachocho can be made easier by adding ethanol to moisten the dried baekhwasachocho and crushing and mixing the raw vinegar into the dough state (step S50).

Thereafter, the second mixture is fermented for 30 to 60 days with stirring at an internal temperature of 20 to 38 ° C. for 3 to 5 hours daily at 40 to 100 times per minute (step S60).

After the fermentation is completed, the second mixture is compressed to extract the fermentation fat and oil composition (hereinafter referred to as "second fermentation fat and oil composition") (step S70).

Then, the first fermented fat and oil composition obtained above and the second fermented fat and oil composition are mixed to complete the anticancer fermented fat and oil composition according to the present embodiment (step S80).

The anticancer fermented fat and oil composition thus prepared is shaped into various amounts of soft capsules. The amount to be shaped into a soft capsule can be determined as needed, for example, in the range of 200 to 1200 mg.

Second Embodiment

Figure 2 is a flow chart showing a second embodiment of the anti-cancer fermented fat and oil composition according to the present invention.

As shown, in the present embodiment is fermented in the state of fermented yeast fruit and baekrye sagechoe made of fermented fat and oil composition fermented with fermented yolk fruit fermented maintenance composition consists of the process of producing an anti-cancer fermented fat and oil composition.

In more detail, first, 1 to 5% by weight of alcohol alcohol is mixed for the journey fruit and the journey fruit as in the first embodiment, and aged for 20 to 30 hours (step S110).

Then, the third mixture of fermented fermented fruit and alcohol is mixed with white chlorosis and vegetable edible oil. At this time, the white phoenix saengcho add about the same weight as the fruit of the journey, and use the water content 20-35% dried. In addition, vegetable edible oil is added 25 to 65% by weight relative to the fruit, alcohol and white birch. In addition, a vacuum emulsifier is used as the fermenter as in the first embodiment (step S120).

Fermentation temperature, fermentation period and stirring conditions are also fermented in the same manner as in the first embodiment (step S130).

The fermented fat and oil composition (hereinafter referred to as "third fermented fat and oil composition") is obtained by pressing the fermented third mixture. And the third fermented fat and oil composition obtained in this way is already mixed state of the fruit and baekryeoksa, the third fermented fat and oil composition is the anti-cancer fermented fat and oil composition according to the present invention (step S140).

The anticancer fermented fat and oil composition thus prepared is also shaped into various amounts of soft capsules.

<Experimental Method>

Since the anticancer fermented fat and oil composition prepared in Example 1 and Example 2 were actually composed of substantially the same components, the following experiment was performed with the anticancer fermented fat and oil composition prepared by the method in Example 1.

The cancer cell lines used in this experiment were purchased from Korean Cell Line Bank as one lung cancer cell line (A549), one breast cancer cell line (MCF-7), and one liver cancer cell line (HepG2).

In addition, cancer cell line proliferation inhibitory activity was evaluated by MTT (3- (4,5-Dimethylthiazol-2-yl) -2,5-diphenyltetrazolium bromide, a tetrazole). That is, cancer cells of 1.5 × 10 ⁵ were added to a 24-well plate using 1 mL of medium per well, and cultured for 24 hours. Then, the anti-cancer fermentation composition was added for each concentration and cultured again for 24 hours. After the cell culture was terminated, the medium was removed and washed twice with PBS solution, 400 uL of MTT reagent was added per well, followed by a reaction for 3 hours. The intensity of formazan dye (purple), a MTT derivative formed by enzymatic reaction of surviving cancer cells, was measured in a 570 nm wavelength microplate reader (Tecan model Sunrise) (reference wavelength 690 nm).

<Experimental Results>

The anti-proliferative activity of three types of cancer cell lines (lung cancer, breast cancer, liver cancer) was investigated using anti-cancer fermented oil and fat composition. The cancer cell proliferation inhibitory activity of anti-cancer fermented oil and fat composition was high in all cancer cell lines.

More specifically, in Figures 3 to 5, 'Conc.' Represents the concentration of the anti-cancer fermented fat and oil composition, and '% of control' represents the population of cancer cells in% relative to the initial population.

At this time, in the case of lung cancer cells (see FIG. 3) and breast cancer cells (see FIG. 4), the number of cells appeared to decrease rapidly at the concentration of 0.125% or more of the anticancer fermentation maintenance composition, and 0.5 for the liver cancer cells (see FIG. 5). A sharp decrease in cell numbers was observed at concentrations above%.

Therefore, the anticancer fermentation and maintenance composition according to the present invention has been shown to have a strong activity of inhibiting cancer cell proliferation, and is expected to be developed as an anticancer or cancer prevention food or a natural drug material.

The rights of the present invention are not limited to the embodiments described above, but are defined by the claims, and those skilled in the art can make various modifications and adaptations within the scope of the claims. It is self-evident.

1 is a flow chart showing a first embodiment of the anti-cancer fermented fat and oil composition according to the present invention,

Figure 2 is a flow chart showing a second embodiment of the anticancer fermentation fat and oil composition according to the present invention,

3 to 5 is a table showing the results of the cancer cell growth inhibitory effect of the anticancer fermentation fat and oil composition according to the present invention.

Claims (6)

Mixing 1 to 5% by weight of alcohol alcohol with respect to the journey fruit and the journey fruit, ripening for 20 to 30 hours; Injecting a first mixture of 25-65% by weight of vegetable edible oil into the fermented fruit and alcohol from the fermenter to the fermenter; Fermenting the first mixture for 30 to 60 days in an oxygen-free state at an internal temperature of 20 to 38 ° C .; Compressing the fermented first mixture to extract a first fermented fat and oil composition; Injecting a second mixture of baekhwasa vinegar dried at 20 to 35% of water content with a vegetable edible oil of the same weight as baekhwasacho vinegar to the fermentor; Fermenting the second mixture for 30 to 60 days in an oxygen-free state at an internal temperature of 20 to 38 ° C .; Compressing the fermented second mixture to extract a second fermented fat and oil composition; And Mixing the first fermented fat and oil composition and the second fermented fat and oil composition. Method of producing an anticancer composition by oil phase fermentation comprising a. Mixing 1 to 5% by weight of alcohol alcohol with respect to the journey fruit and the journey fruit, ripening for 20 to 30 hours; Whitening hydrangea dried with 20 to 35% water content of the same weight as the journey fruit, and 25 to 65% by weight of vegetable edible oil with respect to the journey fruit and alcohol and ethanol; Introducing the mixture into the fermentor; Fermenting the third mixture for 30 to 60 days in an oxygen-free state at an internal temperature of 20 to 38 ° C .; And Compressing the fermented third mixture to extract a third fermented fat and oil composition; a method of producing an anticancer composition by oil phase fermentation, comprising: a. The method according to claim 1 or 2, Frequency of the alcohol alcohol is a method of producing an anticancer composition by oil phase fermentation, characterized in that 15 to 25 degrees. The method according to claim 1 or 2, The fermenter is a vacuum emulsifier, characterized in that the production of anticancer composition by oil phase fermentation. 5. The method of claim 4, In the fermentation step, the method for producing an anticancer composition by oil phase fermentation, characterized in that the first to third mixtures are stirred for 3 to 5 hours every day 40 to 100 times per minute. The method according to claim 1 or 2, The vegetable edible oil, grape seed oil, soybean oil, jade oil, olive oil, pumpkin seed oil, sunflower seed oil, flaxseed oil, a method for producing an anticancer composition by oil phase fermentation, characterized in that the rapeseed oil.

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