CN107518436A - A kind of DHA grease microscapsule powder containing polypeptide and preparation method thereof - Google Patents
A kind of DHA grease microscapsule powder containing polypeptide and preparation method thereof Download PDFInfo
- Publication number
- CN107518436A CN107518436A CN201710758771.0A CN201710758771A CN107518436A CN 107518436 A CN107518436 A CN 107518436A CN 201710758771 A CN201710758771 A CN 201710758771A CN 107518436 A CN107518436 A CN 107518436A
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- CN
- China
- Prior art keywords
- protein
- dha
- polypeptide
- minutes
- protease
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000000843 powder Substances 0.000 title claims abstract description 70
- 108090000765 processed proteins & peptides Proteins 0.000 title claims abstract description 42
- 229920001184 polypeptide Polymers 0.000 title claims abstract description 41
- 102000004196 processed proteins & peptides Human genes 0.000 title claims abstract description 41
- 238000002360 preparation method Methods 0.000 title claims abstract description 22
- 239000004519 grease Substances 0.000 title claims abstract description 21
- 102000004169 proteins and genes Human genes 0.000 claims abstract description 37
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 37
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 12
- 239000003963 antioxidant agent Substances 0.000 claims abstract description 7
- 239000003995 emulsifying agent Substances 0.000 claims abstract description 7
- 230000003078 antioxidant effect Effects 0.000 claims abstract description 5
- 150000001720 carbohydrates Chemical class 0.000 claims abstract description 5
- 235000018102 proteins Nutrition 0.000 claims description 36
- 102000004190 Enzymes Human genes 0.000 claims description 28
- 108090000790 Enzymes Proteins 0.000 claims description 28
- 239000004365 Protease Substances 0.000 claims description 28
- 229940088598 enzyme Drugs 0.000 claims description 28
- 238000003756 stirring Methods 0.000 claims description 22
- 235000013336 milk Nutrition 0.000 claims description 19
- 239000008267 milk Substances 0.000 claims description 19
- 210000004080 milk Anatomy 0.000 claims description 19
- 230000009849 deactivation Effects 0.000 claims description 17
- 239000000203 mixture Substances 0.000 claims description 17
- 102000004407 Lactalbumin Human genes 0.000 claims description 16
- 108090000942 Lactalbumin Proteins 0.000 claims description 16
- 102000009027 Albumins Human genes 0.000 claims description 14
- 108010088751 Albumins Proteins 0.000 claims description 14
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 14
- 239000008346 aqueous phase Substances 0.000 claims description 14
- 239000012071 phase Substances 0.000 claims description 14
- 108090000526 Papain Proteins 0.000 claims description 12
- 235000019834 papain Nutrition 0.000 claims description 12
- 229940055729 papain Drugs 0.000 claims description 12
- 108010004032 Bromelains Proteins 0.000 claims description 11
- 241001494479 Pecora Species 0.000 claims description 11
- 102000057297 Pepsin A Human genes 0.000 claims description 11
- 108090000284 Pepsin A Proteins 0.000 claims description 11
- 235000019835 bromelain Nutrition 0.000 claims description 11
- 229940111202 pepsin Drugs 0.000 claims description 11
- 108010084695 Pea Proteins Proteins 0.000 claims description 10
- 108010073771 Soybean Proteins Proteins 0.000 claims description 10
- 241000209140 Triticum Species 0.000 claims description 10
- 235000021307 Triticum Nutrition 0.000 claims description 10
- 239000012153 distilled water Substances 0.000 claims description 10
- 235000019702 pea protein Nutrition 0.000 claims description 10
- 235000019710 soybean protein Nutrition 0.000 claims description 10
- 108010068370 Glutens Proteins 0.000 claims description 9
- 235000021312 gluten Nutrition 0.000 claims description 9
- 101710180319 Protease 1 Proteins 0.000 claims description 8
- 101710180316 Protease 2 Proteins 0.000 claims description 8
- 101710137710 Thioesterase 1/protease 1/lysophospholipase L1 Proteins 0.000 claims description 8
- 229910052799 carbon Inorganic materials 0.000 claims description 7
- 238000009413 insulation Methods 0.000 claims description 7
- 229910052757 nitrogen Inorganic materials 0.000 claims description 7
- GVJHHUAWPYXKBD-UHFFFAOYSA-N (±)-α-Tocopherol Chemical compound OC1=C(C)C(C)=C2OC(CCCC(C)CCCC(C)CCCC(C)C)(C)CCC2=C1C GVJHHUAWPYXKBD-UHFFFAOYSA-N 0.000 claims description 6
- 229930006000 Sucrose Natural products 0.000 claims description 6
- 239000005720 sucrose Substances 0.000 claims description 6
- 108091005804 Peptidases Proteins 0.000 claims description 5
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims description 5
- 235000006708 antioxidants Nutrition 0.000 claims description 5
- 235000019419 proteases Nutrition 0.000 claims description 5
- 230000007062 hydrolysis Effects 0.000 claims description 4
- 238000006460 hydrolysis reaction Methods 0.000 claims description 4
- 150000008442 polyphenolic compounds Chemical class 0.000 claims description 4
- 235000013824 polyphenols Nutrition 0.000 claims description 4
- -1 sucrose fatty ester Chemical class 0.000 claims description 4
- HDTRYLNUVZCQOY-UHFFFAOYSA-N α-D-glucopyranosyl-α-D-glucopyranoside Natural products OC1C(O)C(O)C(CO)OC1OC1C(O)C(O)C(O)C(CO)O1 HDTRYLNUVZCQOY-UHFFFAOYSA-N 0.000 claims description 3
- JLPULHDHAOZNQI-ZTIMHPMXSA-N 1-hexadecanoyl-2-(9Z,12Z-octadecadienoyl)-sn-glycero-3-phosphocholine Chemical compound CCCCCCCCCCCCCCCC(=O)OC[C@H](COP([O-])(=O)OCC[N+](C)(C)C)OC(=O)CCCCCCC\C=C/C\C=C/CCCCC JLPULHDHAOZNQI-ZTIMHPMXSA-N 0.000 claims description 3
- OWEGMIWEEQEYGQ-UHFFFAOYSA-N 100676-05-9 Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC2C(OC(O)C(O)C2O)CO)O1 OWEGMIWEEQEYGQ-UHFFFAOYSA-N 0.000 claims description 3
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 3
- 239000011786 L-ascorbyl-6-palmitate Substances 0.000 claims description 3
- QAQJMLQRFWZOBN-LAUBAEHRSA-N L-ascorbyl-6-palmitate Chemical compound CCCCCCCCCCCCCCCC(=O)OC[C@H](O)[C@H]1OC(=O)C(O)=C1O QAQJMLQRFWZOBN-LAUBAEHRSA-N 0.000 claims description 3
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 claims description 3
- 229920002774 Maltodextrin Polymers 0.000 claims description 3
- 239000005913 Maltodextrin Substances 0.000 claims description 3
- GUBGYTABKSRVRQ-PICCSMPSSA-N Maltose Natural products O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-PICCSMPSSA-N 0.000 claims description 3
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 3
- HDTRYLNUVZCQOY-WSWWMNSNSA-N Trehalose Natural products O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-WSWWMNSNSA-N 0.000 claims description 3
- 229930003427 Vitamin E Natural products 0.000 claims description 3
- HDTRYLNUVZCQOY-LIZSDCNHSA-N alpha,alpha-trehalose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-LIZSDCNHSA-N 0.000 claims description 3
- 235000010385 ascorbyl palmitate Nutrition 0.000 claims description 3
- WIGCFUFOHFEKBI-UHFFFAOYSA-N gamma-tocopherol Natural products CC(C)CCCC(C)CCCC(C)CCCC1CCC2C(C)C(O)C(C)C(C)C2O1 WIGCFUFOHFEKBI-UHFFFAOYSA-N 0.000 claims description 3
- 239000008101 lactose Substances 0.000 claims description 3
- 229940035034 maltodextrin Drugs 0.000 claims description 3
- 150000003904 phospholipids Chemical class 0.000 claims description 3
- 238000012545 processing Methods 0.000 claims description 3
- 235000010378 sodium ascorbate Nutrition 0.000 claims description 3
- PPASLZSBLFJQEF-RKJRWTFHSA-M sodium ascorbate Substances [Na+].OC[C@@H](O)[C@H]1OC(=O)C(O)=C1[O-] PPASLZSBLFJQEF-RKJRWTFHSA-M 0.000 claims description 3
- 229960005055 sodium ascorbate Drugs 0.000 claims description 3
- PPASLZSBLFJQEF-RXSVEWSESA-M sodium-L-ascorbate Chemical compound [Na+].OC[C@H](O)[C@H]1OC(=O)C(O)=C1[O-] PPASLZSBLFJQEF-RXSVEWSESA-M 0.000 claims description 3
- 229940083466 soybean lecithin Drugs 0.000 claims description 3
- 235000019165 vitamin E Nutrition 0.000 claims description 3
- 229940046009 vitamin E Drugs 0.000 claims description 3
- 239000011709 vitamin E Substances 0.000 claims description 3
- LDVVTQMJQSCDMK-UHFFFAOYSA-N 1,3-dihydroxypropan-2-yl formate Chemical compound OCC(CO)OC=O LDVVTQMJQSCDMK-UHFFFAOYSA-N 0.000 claims description 2
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 2
- 238000013019 agitation Methods 0.000 claims description 2
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 claims description 2
- GUBGYTABKSRVRQ-QUYVBRFLSA-N beta-maltose Chemical compound OC[C@H]1O[C@H](O[C@H]2[C@H](O)[C@@H](O)[C@H](O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@@H]1O GUBGYTABKSRVRQ-QUYVBRFLSA-N 0.000 claims description 2
- 239000008103 glucose Substances 0.000 claims description 2
- 230000003301 hydrolyzing effect Effects 0.000 claims description 2
- 244000099147 Ananas comosus Species 0.000 claims 1
- 235000007119 Ananas comosus Nutrition 0.000 claims 1
- 241000165940 Houjia Species 0.000 claims 1
- 150000001875 compounds Chemical class 0.000 claims 1
- 239000000463 material Substances 0.000 abstract description 10
- 235000020207 hydrolysed-protein milk formula Nutrition 0.000 abstract description 5
- 235000007164 Oryza sativa Nutrition 0.000 abstract description 4
- 235000013350 formula milk Nutrition 0.000 abstract description 4
- 235000009566 rice Nutrition 0.000 abstract description 4
- 239000007787 solid Substances 0.000 abstract description 4
- 235000013361 beverage Nutrition 0.000 abstract description 3
- 238000002156 mixing Methods 0.000 abstract description 3
- 235000015895 biscuits Nutrition 0.000 abstract description 2
- 235000020124 milk-based beverage Nutrition 0.000 abstract description 2
- 235000008476 powdered milk Nutrition 0.000 abstract description 2
- 240000007594 Oryza sativa Species 0.000 abstract 1
- 239000011162 core material Substances 0.000 abstract 1
- 238000004945 emulsification Methods 0.000 abstract 1
- 230000007065 protein hydrolysis Effects 0.000 abstract 1
- MBMBGCFOFBJSGT-KUBAVDMBSA-N all-cis-docosa-4,7,10,13,16,19-hexaenoic acid Chemical compound CC\C=C/C\C=C/C\C=C/C\C=C/C\C=C/C\C=C/CCC(O)=O MBMBGCFOFBJSGT-KUBAVDMBSA-N 0.000 description 86
- 235000020669 docosahexaenoic acid Nutrition 0.000 description 45
- 229940090949 docosahexaenoic acid Drugs 0.000 description 43
- 239000003094 microcapsule Substances 0.000 description 15
- 238000005516 engineering process Methods 0.000 description 9
- 238000012360 testing method Methods 0.000 description 8
- 230000000052 comparative effect Effects 0.000 description 7
- 238000009658 destructive testing Methods 0.000 description 6
- 239000012530 fluid Substances 0.000 description 6
- 150000002978 peroxides Chemical class 0.000 description 6
- 238000005303 weighing Methods 0.000 description 6
- 238000000265 homogenisation Methods 0.000 description 5
- YZXBAPSDXZZRGB-DOFZRALJSA-N arachidonic acid Chemical compound CCCCC\C=C/C\C=C/C\C=C/C\C=C/CCCC(O)=O YZXBAPSDXZZRGB-DOFZRALJSA-N 0.000 description 4
- 238000013475 authorization Methods 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 235000020777 polyunsaturated fatty acids Nutrition 0.000 description 4
- 210000004885 white matter Anatomy 0.000 description 4
- 241000209094 Oryza Species 0.000 description 3
- 230000033228 biological regulation Effects 0.000 description 3
- 210000004556 brain Anatomy 0.000 description 3
- 235000014633 carbohydrates Nutrition 0.000 description 3
- 230000006872 improvement Effects 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- DVSZKTAMJJTWFG-SKCDLICFSA-N (2e,4e,6e,8e,10e,12e)-docosa-2,4,6,8,10,12-hexaenoic acid Chemical compound CCCCCCCCC\C=C\C=C\C=C\C=C\C=C\C=C\C(O)=O DVSZKTAMJJTWFG-SKCDLICFSA-N 0.000 description 2
- GZJLLYHBALOKEX-UHFFFAOYSA-N 6-Ketone, O18-Me-Ussuriedine Natural products CC=CCC=CCC=CCC=CCC=CCC=CCCCC(O)=O GZJLLYHBALOKEX-UHFFFAOYSA-N 0.000 description 2
- 241000195493 Cryptophyta Species 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 2
- JAZBEHYOTPTENJ-JLNKQSITSA-N all-cis-5,8,11,14,17-icosapentaenoic acid Chemical compound CC\C=C/C\C=C/C\C=C/C\C=C/C\C=C/CCCC(O)=O JAZBEHYOTPTENJ-JLNKQSITSA-N 0.000 description 2
- 235000021342 arachidonic acid Nutrition 0.000 description 2
- 229940114079 arachidonic acid Drugs 0.000 description 2
- 230000029087 digestion Effects 0.000 description 2
- KAUVQQXNCKESLC-UHFFFAOYSA-N docosahexaenoic acid (DHA) Natural products COC(=O)C(C)NOCC1=CC=CC=C1 KAUVQQXNCKESLC-UHFFFAOYSA-N 0.000 description 2
- 235000020673 eicosapentaenoic acid Nutrition 0.000 description 2
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- JAZBEHYOTPTENJ-UHFFFAOYSA-N eicosapentaenoic acid Natural products CCC=CCC=CCC=CCC=CCC=CCCCC(O)=O JAZBEHYOTPTENJ-UHFFFAOYSA-N 0.000 description 2
- 238000005538 encapsulation Methods 0.000 description 2
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- YUFFSWGQGVEMMI-JLNKQSITSA-N (7Z,10Z,13Z,16Z,19Z)-docosapentaenoic acid Chemical compound CC\C=C/C\C=C/C\C=C/C\C=C/C\C=C/CCCCCC(O)=O YUFFSWGQGVEMMI-JLNKQSITSA-N 0.000 description 1
- 235000021294 Docosapentaenoic acid Nutrition 0.000 description 1
- 244000068988 Glycine max Species 0.000 description 1
- 235000010469 Glycine max Nutrition 0.000 description 1
- OYHQOLUKZRVURQ-HZJYTTRNSA-N Linoleic acid Chemical compound CCCCC\C=C/C\C=C/CCCCCCCC(O)=O OYHQOLUKZRVURQ-HZJYTTRNSA-N 0.000 description 1
- 229920000881 Modified starch Polymers 0.000 description 1
- 239000004368 Modified starch Substances 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 108010009736 Protein Hydrolysates Proteins 0.000 description 1
- 235000010489 acacia gum Nutrition 0.000 description 1
- 239000001785 acacia senegal l. willd gum Substances 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 235000020661 alpha-linolenic acid Nutrition 0.000 description 1
- 230000001458 anti-acid effect Effects 0.000 description 1
- 230000003110 anti-inflammatory effect Effects 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- OGBUMNBNEWYMNJ-UHFFFAOYSA-N batilol Chemical class CCCCCCCCCCCCCCCCCCOCC(O)CO OGBUMNBNEWYMNJ-UHFFFAOYSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- SQVRNKJHWKZAKO-UHFFFAOYSA-N beta-N-Acetyl-D-neuraminic acid Natural products CC(=O)NC1C(O)CC(O)(C(O)=O)OC1C(O)C(O)CO SQVRNKJHWKZAKO-UHFFFAOYSA-N 0.000 description 1
- 210000004204 blood vessel Anatomy 0.000 description 1
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- 238000011161 development Methods 0.000 description 1
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- 229930195729 fatty acid Natural products 0.000 description 1
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- 238000009472 formulation Methods 0.000 description 1
- 239000000446 fuel Substances 0.000 description 1
- 235000020664 gamma-linolenic acid Nutrition 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 239000000787 lecithin Substances 0.000 description 1
- 235000010445 lecithin Nutrition 0.000 description 1
- 235000020778 linoleic acid Nutrition 0.000 description 1
- OYHQOLUKZRVURQ-IXWMQOLASA-N linoleic acid Natural products CCCCC\C=C/C\C=C\CCCCCCCC(O)=O OYHQOLUKZRVURQ-IXWMQOLASA-N 0.000 description 1
- 230000014759 maintenance of location Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 235000012054 meals Nutrition 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 125000002496 methyl group Chemical group [H]C([H])([H])* 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23P—SHAPING OR WORKING OF FOODSTUFFS, NOT FULLY COVERED BY A SINGLE OTHER SUBCLASS
- A23P10/00—Shaping or working of foodstuffs characterised by the products
- A23P10/30—Encapsulation of particles, e.g. foodstuff additives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
- A23C9/00—Milk preparations; Milk powder or milk powder preparations
- A23C9/152—Milk preparations; Milk powder or milk powder preparations containing additives
- A23C9/1526—Amino acids; Peptides; Protein hydrolysates; Nucleic acids; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J3/00—Working-up of proteins for foodstuffs
- A23J3/30—Working-up of proteins for foodstuffs by hydrolysis
- A23J3/32—Working-up of proteins for foodstuffs by hydrolysis using chemical agents
- A23J3/34—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes
- A23J3/341—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes of animal proteins
- A23J3/343—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes of animal proteins of dairy proteins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J3/00—Working-up of proteins for foodstuffs
- A23J3/30—Working-up of proteins for foodstuffs by hydrolysis
- A23J3/32—Working-up of proteins for foodstuffs by hydrolysis using chemical agents
- A23J3/34—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes
- A23J3/346—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes of vegetable proteins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/385—Concentrates of non-alcoholic beverages
- A23L2/39—Dry compositions
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/52—Adding ingredients
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/115—Fatty acids or derivatives thereof; Fats or oils
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/115—Fatty acids or derivatives thereof; Fats or oils
- A23L33/12—Fatty acids or derivatives thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/17—Amino acids, peptides or proteins
- A23L33/18—Peptides; Protein hydrolysates
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/17—Amino acids, peptides or proteins
- A23L33/185—Vegetable proteins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/17—Amino acids, peptides or proteins
- A23L33/19—Dairy proteins
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Abstract
The invention provides a kind of DHA grease microscapsule powder containing polypeptide and preparation method thereof.The microscapsule powder includes each component of following weight percentage：Polypeptide 20.3 41.2%, protein 8.8 29.7%, carbohydrate 8.8 28.8%, DHA algal oil 20 40%, emulsifying agent 1%, antioxidant 0.2%.The preparation method is by moderate control protein hydrolysis degree, obtains the mixing wall material of albumen and polypeptide, then the emulsification of wall material, core, emulsifying agent and antioxidant is mixed, be high-pressure homogeneous, is finally spray-dried and produces.Polypeptide is rich in DHA microscapsule powder wall materials prepared by the present invention, it is easier to digesting and assimilating for human body, it can be applied not only in the formula such as ordinary powdered milk, ground rice, biscuit, milk beverage, it can also be applied in protein hydrolysate formula, directly it can more be taken after mixing it with water as solid beverage, particularly suitable children and mid-aged population are eaten.
Description
Technical field
The present invention relates to a kind of DHA grease microscapsule powder containing polypeptide and preparation method thereof, belong to food processing
Technical field.
Background technology
Polyunsaturated fatty acid refers to containing two or more double bonds, and carbon chain lengths are the straight of 18-22 carbon atom
Chain fatty acid.According to the position of first double-linked carbon in methyl end, polyunsaturated fatty acid can be divided into ω -3 and ω -6 series
Polyunsaturated fatty acid.ω-3：Alpha-linolenic acid (ALA), eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA)；ω-
6：Linoleic acid (LA), gamma-Linolenic acid (GLA) and arachidonic acid (ARA).
Docosahexaenoic acid (DHA) belongs to a kind of important polyunsaturated fatty acid of ω -3 series, is brain and regards
The important composition composition of nethike embrane, is commonly called as " docosapentaenoic acid ", and can maintain and promote nerve growth.DHA is in human brain cortex
Middle content is up to 20%, proportion about 50% in eye retina, most important to tire infant intelligence and visual acuity.Through
After scientific validation for many years, it is determined that DHA in brain tonic and intelligence development, softening blood vessel, anti inflammatory immunity, the effect for suppressing cancer etc.,
Therefore, in the diet of people, a certain amount of DHA is rationally added into meals and has been subjected to extensive concern and has paid attention to,
Thus nutritive value that DHA has is increasingly notable with commercial significance.
DHA is applied in baby milk powder primarily as new nutrition fortifier in a manner of microcapsule embedded at present.
Microcapsules technology be it is a kind of solid or liquid are embedded with filmogen, so as to protect core from adverse environmental factors such as
Temperature, humidity, oxygen etc. influence, to improve the stability of encapsulation object and shelf life, a kind of control of extension encapsulation object application
Release technology.Unrighted acid is coated using microcapsules technology, so as to improve the stability of grease, makes its nutritive value
Maximize the retention, have broad application prospects with flavor.
The main wall material of existing DHA microscapsule powders has two kinds, and one kind is protein, and another kind is polysaccharide, as patent is public
Cloth number be CN102228257A algae oil DHA microcapsules and preparation method thereof, Patent publication No CN104906071A using DHA grease as
A kind of microcapsules containing sialic acid and DHA of the production technology of waste DHA microcapsules, patent authorization number CN104041827B
And preparation method thereof, Patent publication No CN104621570A high stability algae oil DHA microscapsule powders and preparation method thereof etc., its
Wall material is modified starch and Arabic gum；Such as a kind of anti-acid microalgae DHA grease microcapsules of patent authorization number CN104432059B
A kind of DHA microcapsules of powder, patent authorization number CN101884415B and its preparation technology, its wall material are mainly protein；Also
Be to be mixed using protein and carbohydrate as wall material, such as a kind of high fuel loads of patent authorization number CN103549442B
DHA algal oil microcapsule powder and its preparation technology, the preparation technology of Patent publication No CN101878903A new DHA microcapsules.This
It can be added in normal milk powder or ground rice using protein or polysaccharide as the DHA microcapsules of wall material a bit, for digestion and absorption function
Normal crowd eats, but for constitution is poor, the unsound crowd of digestion and absorption function such as albumen does not tolerate infant, old
Year people, major operation patient etc., normal milk powder or ground rice do not apply to simultaneously, and at this moment the milk powder of protein hydrolysate formula is exactly optimal choosing
Select, and the DHA microscapsule powders of traditional wall material can not be applied in protein hydrolysate formula milk well.
In order to meet the needs of protein hydrolysate formula milk market, the present invention is made by carrying out appropriate enzymolysis to protein
It turns into the mixture of a kind of polypeptide and albumen, then for embedding DHA grease, so that it may apply to protein hydrolysate
In formula milk；Product of the present invention can also be eaten, widen directly as solid beverage for children, mid-aged population simultaneously
The application of DHA microscapsule powders.
The content of the invention
In view of the shortcomings of the prior art, the invention provides a kind of DHA grease microcapsule powder powder formulation containing polypeptide
And preparation method thereof.The present invention is using protein and polypeptide as microcapsule wall material, using DHA algal oil as core, then adds certain
The emulsifying agent and antioxidant of amount, are made by special process flow.
The purpose of the present invention is achieved through the following technical solutions：
In a first aspect, the invention provides a kind of DHA grease microscapsule powder containing polypeptide, including following quality hundred
Divide each component of content：
The too high levels of the polypeptide, then it can reduce embedding effect.
Preferably, the protein is in milk albumin, sheep lactalbumin, soybean protein, wheat gluten, pea protein
Any one or a few.
Preferably, the polypeptide is that protein is made by protease hydrolytic；The protein be milk albumin,
Any one or a few in sheep lactalbumin, soybean protein, wheat gluten, pea protein；The protease be trypsase,
One or more in pepsin, papain, bromelain.
Preferably, when the protein is milk albumin and/or sheep lactalbumin, with trypsase and papain
It is combined hydrolysis；When the protein is at least one of soybean protein, wheat gluten, pea protein, pepsin is used
Hydrolysis is combined with bromelain.
Preferably, the carbohydrate is one in lactose, maltodextrin, maltose, sucrose, trehalose, glucose
Kind or several mixtures.
Preferably, the emulsifying agent is monoglyceride, soybean lecithin, modified phospholipid, one kind in sucrose fatty ester or several
The mixture of kind example；The antioxidant is one kind in Tea Polyphenols, ascorbyl palmitate, sodium ascorbate, vitamin E
Or several mixture.
Second aspect, the invention provides a kind of preparation method of the DHA grease microscapsule powder containing polypeptide, including
Following steps：
A, protein is not exclusively digested, prepares the mixture of protein and polypeptide；
B, carbohydrate, emulsifying agent are added in the mixture of protein and polypeptide made from step A, stirred
Uniformly, and at 70~80 DEG C 20~30 minutes are incubated, obtain aqueous phase；
C, add antioxidants in DHA algal oil and stir, in the closed container of inflated with nitrogen, be added to 60~80
30~60 minutes are incubated at DEG C, obtains oil phase；
D, under agitation, oil phase is squeezed into aqueous phase, is sheared 3~5 minutes with cutter, be synchronously cooled to 60 DEG C,
Then homogeneous 2~3 times under the conditions of pressure is 25~45MPa, then be spray-dried and produce the DHA microscapsule powders.
Preferably, in step A, when the protein is milk albumin and/or sheep lactalbumin, specific preparation method is such as
Under：
A1, protein is weighed, add 2~4 times of distilled water to stir and be heated to 37 DEG C, albumen is fully dissolved；
A2, protease 1 is added in solution made from step A1, hydrolyzed 5~15 minutes, be then heated to 80 DEG C, insulation
Enzyme deactivation in 20 minutes；
A3, the solution after step A2 processing is cooled to 55~60 DEG C, adds protease 2, hydrolyze 25~30 minutes, so
After be heated to 80 DEG C, be incubated enzyme deactivation in 20 minutes；Produce the mixture of the protein and polypeptide；
The protease 1 is trypsase or pepsin；Protease 2 is papain or bromelain.
Preferably, when the protein is milk albumin and/or sheep lactalbumin, protease 1 is trypsase, and enzyme adds
It is 4000~8000U/g to enter concentration；Protease 2 is papain, and it is 5000~10000U/g that enzyme, which adds concentration,.
Preferably, when the protein is at least one of soybean protein, wheat gluten, pea protein, protease 1 is
Pepsin, it is 2000~4000U/g that enzyme, which adds concentration,；Protease 2 is bromelain, enzyme add concentration be 4000~
8000U/g。
Preferably, when the protein is at least one of soybean protein, wheat gluten, pea protein, in step A1,
The pH value of solution need to be adjusted to 2.
Prior art is compared, and the present invention has following beneficial effect：
1st, zymotechnic is applied in the microcapsule embedded technical fields of DHA by the present invention first.
2nd, it is rich in polypeptide in DHA microscapsule powder bars prepared by the present invention, it is easier to which human body is digested and assimilated, no
It can be applied only in the formula such as ordinary powdered milk, ground rice, biscuit, milk beverage, can also be applied in protein hydrolysate formula, more may be used
Directly to be taken after mixing it with water as solid beverage, DHA application is widened, particularly suitable children and mid-aged population are eaten.
3rd, the present invention passes through controlled enzymatic hydrolysis degree so that polypeptide content is 20.3-41.2%, and protein content is
8.8-29.7%, so that the microcapsule embedded effects of DHA are more preferable, application is wider.
Embodiment
With reference to specific embodiment, the present invention is described in detail.Following examples will be helpful to the technology of this area
Personnel further understand the present invention, but the invention is not limited in any way.It should be pointed out that the ordinary skill to this area
For personnel, without departing from the inventive concept of the premise, various modifications and improvements can be made.These belong to the present invention
Protection domain.
Embodiment 1
(1) 5kg milk albumins are weighed, adds 10kg distilled water to stir and is heated to 37 DEG C, make lactalbumin fully molten
Solution, the trypsase of 20,000,000 units is added, hydrolyze 5 minutes, mixed liquor is heated to 80 DEG C afterwards, be incubated enzyme deactivation in 20 minutes；
Fluid temperature is down to 60 DEG C again, adds the papain of 25,000,000 units, hydrolyzes 30 minutes, is afterwards heated to mixed liquor
80 DEG C, it is incubated enzyme deactivation in 20 minutes.
(2) weigh 0.88kg lactose, 0.1kg monoglycerides are added in (1), stir and in 70 DEG C, be incubated 20 minutes,
This is aqueous phase.
(3) Tea Polyphenols for weighing 0.02kg is added in 4kg DHA algal oil and stirred, in the closed container of nitrogen is rushed,
60 DEG C are heated to, is incubated 30 minutes, this is oil phase.
(4) oil phase is squeezed into aqueous phase under conditions of stirring, is sheared 5 minutes with cutter, be synchronously cooled to 60 DEG C, it
Homogeneous 3 times under the conditions of homogenization pressure is 45MP afterwards, are finally spray-dried and 8.6kgDHA microscapsule powders are made, after testing albumen
The gross mass that matter accounts for microscapsule powder gross weight is 29.7%, and polypeptide (molecular weight≤10000) accounts for microscapsule powder gross weight
The 20.3% of amount, surface oil content 0.1%, powder color is milky.62 DEG C of destructive testings, peroxide value after the 45th day
2.1meq/kg, powder color slightly turn yellow than before, are faint yellow.
Embodiment 2
(1) 5kg sheep lactalbumin is weighed, 20kg distilled water stirs and is heated to 37 DEG C, makes lactalbumin fully molten
Solution, the trypsase of 40,000,000 units is first added, 15 minutes, mixed liquor is heated to 80 DEG C afterwards, is incubated enzyme deactivation in 20 minutes；Again
Fluid temperature is down to 60 DEG C, adds the papain of 50,000,000 units, hydrolyzes 30 minutes, mixed liquor is heated to 80 afterwards
DEG C, it is incubated enzyme deactivation in 20 minutes.
(2) weigh 2.88kg maltodextrin, 0.1kg soybean lecithins are added in (1), stir and in 80 DEG C, be incubated
30 minutes, this was aqueous phase.
(3) ascorbyl palmitate for weighing 0.02kg is added in 2kg DHA algal oil and stirred, and is rushing nitrogen
In closed container, 80 DEG C are heated to, is incubated 60m minutes, this is oil phase.
(4) oil phase is squeezed into aqueous phase under conditions of stirring, is sheared 3 minutes with cutter, be synchronously cooled to 60 DEG C, it
Homogeneous 2 times under the conditions of homogenization pressure is 25MP afterwards, are finally spray-dried the DHA microscapsule powders that 8.5kg is made, after testing egg
The gross mass that white matter accounts for microscapsule powder gross weight is 9.9%, and polypeptide (molecular weight≤10000) accounts for microscapsule powder gross weight
The 40.1% of amount, surface oil 0.56%, powder color is milky.62 DEG C of destructive testings, peroxide value after the 45th day
3.6meq/kg, powder color slightly turn yellow than before, are faint yellow.
Embodiment 3
(1) 5kg soybean protein is weighed, adds 10kg distilled water to stir and is heated to 37 DEG C, regulation PH to 2, addition
The pepsin of 10000000 units, hydrolyze 5 minutes, mixed liquor is heated to 80 DEG C afterwards, be incubated enzyme deactivation in 20 minutes；Again by liquid
Temperature is down to 55 DEG C, adds the bromelain of 20,000,000 units, hydrolyzes 25 minutes, mixed liquor is heated into 80 DEG C afterwards, protect
Warm enzyme deactivation in 20 minutes.
(2) weigh 0.88kg maltose, 0.1kg modified phospholipid is added in (1), stir and in 70 DEG C, be incubated
20 minutes, this was aqueous phase.
(3) sodium ascorbate for weighing 0.02kg is added in 4kg DHA algal oil and stirred, and is rushing the closed appearance of nitrogen
In device, 80 DEG C are heated to, is incubated 60 minutes, this is oil phase.
(4) oil phase is squeezed into aqueous phase under conditions of stirring, is sheared 5 minutes with cutter, be synchronously cooled to 60 DEG C, it
Homogeneous 3 times under the conditions of homogenization pressure is 45MP afterwards, are finally spray-dried the DHA microscapsule powders that 8.7kg is made, after testing egg
The gross mass that white matter accounts for microscapsule powder gross weight is 28.8%, and it is total that polypeptide (molecular weight≤10000) accounts for microscapsule powder
The 21.2% of weight, surface oil 0.12%, powder color are milky.62 DEG C of destructive testings, peroxide value after the 45th day
2.6meq/kg, powder color slightly turn yellow than before, are faint yellow.
Embodiment 4
(1) 5kg wheat gluten is weighed, adds 20kg distilled water to stir and is heated to 37 DEG C, regulation PH to 2, addition
The pepsin of 20000000 units, hydrolyze 10 minutes, mixed liquor is heated to 80 DEG C afterwards, be incubated enzyme deactivation in 20 minutes.Again by liquid
Temperature is down to 55 DEG C, adds the bromelain of 40,000,000 units, hydrolyzes 25 minutes, mixed liquor is heated into 80 DEG C afterwards,
It is incubated enzyme deactivation in 20 minutes.
(2) weigh 2.88kg sucrose, 0.1kg sucrose fatty ester is added in (1), stir and in 80 DEG C, protect
Temperature 30 minutes, this is aqueous phase.
(3) vitamin E for weighing 0.02kg is added in 2kg DHA algal oil and stirred, and is rushing the closed container of nitrogen
In, 70 DEG C are heated to, is incubated 45 minutes, this is oil phase.
(4) oil phase is squeezed into aqueous phase under conditions of stirring, is sheared 3 minutes with cutter, be synchronously cooled to 60 DEG C, it
Homogeneous 2 times under the conditions of homogenization pressure is 30MP afterwards, are finally spray-dried the DHA microscapsule powders that 8.2kg is made, after testing egg
The gross mass that white matter accounts for microscapsule powder gross weight is 8.8%, and polypeptide (molecular weight≤10000) accounts for microscapsule powder gross weight
The 41.2% of amount, surface oil 0.64%, powder color is milky.62 DEG C of destructive testings, peroxide value after the 45th day
4.2meq/kg, powder color slightly turn yellow than before, are faint yellow.
Embodiment 5
(1) pea protein for weighing 5kg adds 15kg distilled water to stir and is heated to 37 DEG C, regulation PH to 2, addition 1500
The pepsin of ten thousand units, hydrolyze 8 minutes, mixed liquor is heated to 80 DEG C afterwards, be incubated enzyme deactivation in 20 minutes.Again by fluid temperature
55 DEG C are down to, adds the bromelain of 30,000,000 units, hydrolyzes 25 minutes, mixed liquor is heated to 80 DEG C afterwards, insulation 20
Minute enzyme deactivation.
(2) 1.88kg trehalose, 0.1kg soybean lecithin are weighed in (1), is stirred and in 75 DEG C, insulation 25
Minute, this is aqueous phase.
(3) Tea Polyphenols for weighing 0.02kg is added in 3kg DHA algal oil and stirred, in the closed container of nitrogen is rushed,
70 DEG C are heated to, is incubated 45 minutes, this is oil phase.
(4) oil phase is squeezed into aqueous phase under conditions of stirring, is sheared 4 minutes with cutter, be synchronously cooled to 60 DEG C, it
Homogeneous 3 times under the conditions of homogenization pressure is 30MP afterwards, are finally spray-dried the DHA microscapsule powders that 8.9kg is made, after testing egg
The gross mass that white matter accounts for microscapsule powder gross weight is 17.5%, and it is total that polypeptide (molecular weight≤10000) accounts for microscapsule powder
The 32.5% of weight, surface oil 0.25%, powder color are milky.62 DEG C of destructive testings, peroxide value after the 45th day
3.1meq/kg, powder color slightly turn yellow than before, are faint yellow.
Comparative example 1
The preparation method of this comparative example and embodiment 1 is essentially identical, the difference is that only：In step (1), 5kg is weighed
Milk albumin, add 10kg distilled water to stir and be heated to 37 DEG C, lactalbumin is fully dissolved, add 20,000,000 units
Trypsase, hydrolyze 2 minutes, mixed liquor is heated to 80 DEG C afterwards, be incubated enzyme deactivation in 20 minutes；Fluid temperature is down to 60 again
DEG C, the papain of 25,000,000 units is added, hydrolyzes 30 minutes, mixed liquor is heated to 80 DEG C afterwards, insulation is gone out for 20 minutes
Enzyme.
The thus obtained DHA microscapsule powders gross mass that protein accounts for microscapsule powder gross weight after testing is
39.4%, polypeptide (molecular weight≤10000) accounts for the 10.6% of microscapsule powder gross weight, surface oil 0.2%, powder face
Color is milky.62 DEG C of destructive testings, peroxide value 2.8meq/kg after the 45th day, powder color change very greatly than before, are
Dark yellow, analyze reason, it may be possible to which polypeptide has color-protecting function under the high temperature conditions.
Comparative example 2
The preparation method of this comparative example and embodiment 1 is essentially identical, the difference is that only：In step (1), 5kg is weighed
Milk albumin, add 10kg distilled water to stir and be heated to 37 DEG C, lactalbumin is fully dissolved, add 50,000,000 units
Trypsase, hydrolyze 15 minutes, mixed liquor is heated to 80 DEG C afterwards, be incubated enzyme deactivation in 20 minutes；Fluid temperature is down to 60 again
DEG C, the papain of 25,000,000 units is added, hydrolyzes 30 minutes, mixed liquor is heated to 80 DEG C afterwards, insulation is gone out for 20 minutes
Enzyme.
The thus obtained DHA microscapsule powders gross mass that protein accounts for microscapsule powder gross weight after testing is 4.6%,
Polypeptide (molecular weight≤10000) accounts for the 45.4% of microscapsule powder gross weight, and surface oil is 8.4% (general surface oil height
In 1%, that is, think no embedded), because surface oil is too high, do not do necessity of stability.
Microscapsule powder made from this comparative example embeds poor effect, and lipochondrion is found very with microscope oil mirror microscopy
Greatly.
Comparative example 3
The preparation method of this comparative example and embodiment 1 is essentially identical, the difference is that only：In step (1), 5kg is weighed
Milk albumin, add 10kg distilled water to stir and be heated to 37 DEG C, lactalbumin is fully dissolved, add 20,000,000 units
Trypsase, hydrolyze 5 minutes, mixed liquor is heated to 80 DEG C afterwards, be incubated enzyme deactivation in 20 minutes；Fluid temperature is down to 60 again
DEG C, the bromelain of 25,000,000 units is added, hydrolyzes 30 minutes, mixed liquor is heated to 80 DEG C afterwards, insulation is gone out for 20 minutes
Enzyme.
The thus obtained DHA microscapsule powders gross mass that protein accounts for microscapsule powder gross weight after testing is 2.8%,
Polypeptide (molecular weight≤10000) accounts for the 47.2% of microscapsule powder gross weight, surface oil 18.2%, due to surface oil too
Height, necessity of stability is not done.
Concrete application approach of the present invention is a lot, and described above is only the preferred embodiment of the present invention.More than it should be pointed out that
Embodiment is merely to illustrate the present invention, and the protection domain being not intended to limit the invention.For the common skill of the art
For art personnel, under the premise without departing from the principles of the invention, some improvement can also be made, these improvement also should be regarded as this hair
Bright protection domain.
Claims (10)
1. a kind of DHA grease microscapsule powder containing polypeptide, it is characterised in that include each group of following weight/mass percentage composition
Point：
2. the DHA grease microscapsule powder according to claim 1 containing polypeptide, it is characterised in that the protein
For any one or a few in milk albumin, sheep lactalbumin, soybean protein, wheat gluten, pea protein.
3. the DHA grease microscapsule powder according to claim 1 containing polypeptide, it is characterised in that the albumen is more
Peptide is that protein is made by protease hydrolytic；The protein is milk albumin, sheep lactalbumin, soybean protein, wheat
Any one or a few in albumen, pea protein；The protease is trypsase, pepsin, papain, pineapple
One or more in protease.
4. the DHA grease microscapsule powder according to claim 3 containing polypeptide, it is characterised in that the protein
For milk albumin and/or sheep lactalbumin when, be combined hydrolysis with trypsase and papain；The protein is
When at least one of soybean protein, wheat gluten, pea protein, hydrolysis is combined with pepsin and bromelain.
5. the DHA grease microscapsule powder according to claim 1 containing polypeptide, it is characterised in that the carbon aquation
Compound is one or more of mixtures in lactose, maltodextrin, maltose, sucrose, trehalose, glucose.
6. the DHA grease microscapsule powder according to claim 1 containing polypeptide, it is characterised in that the emulsifying agent
For the mixture of one or more of examples in monoglyceride, soybean lecithin, modified phospholipid, sucrose fatty ester；The antioxidant
For one or more of mixtures in Tea Polyphenols, ascorbyl palmitate, sodium ascorbate, vitamin E.
7. a kind of preparation method of the DHA grease microscapsule powder according to claim 1 containing polypeptide, its feature exist
In comprising the following steps：
A, protein is not exclusively digested, prepares the mixture of protein and polypeptide；
B, carbohydrate, emulsifying agent are added in the mixture of protein and polypeptide made from step A, stirring is equal
It is even, and 20~30 minutes are incubated at 70~80 DEG C, obtain aqueous phase；
C, add antioxidants in DHA algal oil and stir, in the closed container of inflated with nitrogen, be added at 60~80 DEG C
Insulation 30~60 minutes, obtains oil phase；
D, under agitation, oil phase is squeezed into aqueous phase, is sheared 3~5 minutes with cutter, be synchronously cooled to 60 DEG C, then
The homogeneous 2~3 times under the conditions of pressure is 25~45MPa, then be spray-dried and produce the DHA microscapsule powders.
8. the preparation method of the DHA grease microscapsule powder according to claim 7 containing polypeptide, it is characterised in that
In step A, when the protein is milk albumin and/or sheep lactalbumin, specific preparation method is as follows：
A1, protein is weighed, add 2~4 times of distilled water to stir and be heated to 37 DEG C, albumen is fully dissolved；
A2, protease 1 is added in solution made from step A1, hydrolyzed 5~15 minutes, be then heated to 80 DEG C, be incubated 20 points
Clock enzyme deactivation；
A3, the solution after step A2 processing is cooled to 55~60 DEG C, adds protease 2, hydrolyze 25~30 minutes, Ran Houjia
Heat is incubated enzyme deactivation in 20 minutes to 80 DEG C；Produce the mixture of the protein and polypeptide；
The protease 1 is trypsase or pepsin；Protease 2 is papain or bromelain.
9. the preparation method of the DHA grease microscapsule powder according to claim 8 containing polypeptide, it is characterised in that
When the protein is milk albumin and/or sheep lactalbumin, protease 1 is trypsase, enzyme add concentration be 4000~
8000U/g；Protease 2 is papain, and it is 5000~10000U/g that enzyme, which adds concentration,.
10. the preparation method of the DHA grease microscapsule powder according to claim 8 containing polypeptide, it is characterised in that
When the protein is at least one of soybean protein, wheat gluten, pea protein, protease 1 is pepsin, and enzyme adds
Concentration is 2000~4000U/g；Protease 2 is bromelain, and it is 4000~8000U/g that enzyme, which adds concentration,.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108260807A (en) * | 2018-01-25 | 2018-07-10 | 郑州市云鼎汇砂生物科技有限公司 | It is a kind of that there is fermented food of the healthcare function containing small-molecular peptides |
| CN109123660A (en) * | 2018-09-17 | 2019-01-04 | 山西欧莱特农业科技有限公司 | A kind of walnut lactalbumin composite powder and preparation method thereof for maternal nutritional product |
| CN109198045A (en) * | 2018-10-09 | 2019-01-15 | 嘉必优生物技术（武汉）股份有限公司 | A kind of grease microcapsule and preparation method thereof |
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| CN112450390A (en) * | 2020-10-30 | 2021-03-09 | 浙江波拉波拉食品股份有限公司 | Fish sausage containing DHA microcapsules and preparation method thereof |
| CN115553466A (en) * | 2022-09-30 | 2023-01-03 | 南京益唯森生物科技有限公司 | Nitraria seed oil microcapsule powder containing flax polypeptide and preparation method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108260807A (en) * | 2018-01-25 | 2018-07-10 | 郑州市云鼎汇砂生物科技有限公司 | It is a kind of that there is fermented food of the healthcare function containing small-molecular peptides |
| WO2020024644A1 (en) * | 2018-08-03 | 2020-02-06 | 梁云 | Method for improving stability and safety of microbial oils and fats |
| CN109123660A (en) * | 2018-09-17 | 2019-01-04 | 山西欧莱特农业科技有限公司 | A kind of walnut lactalbumin composite powder and preparation method thereof for maternal nutritional product |
| CN109123660B (en) * | 2018-09-17 | 2021-07-23 | 山西欧莱特农业科技有限公司 | Walnut whey protein composite powder for pregnant woman nutriments and preparation method thereof |
| CN109198045A (en) * | 2018-10-09 | 2019-01-15 | 嘉必优生物技术（武汉）股份有限公司 | A kind of grease microcapsule and preparation method thereof |
| CN109673850A (en) * | 2019-01-31 | 2019-04-26 | 石家庄石牧动物药业有限公司 | A kind of Multifunctional powder grease and preparation method thereof |
| CN110547457A (en) * | 2019-09-02 | 2019-12-10 | 嘉必优生物技术（武汉）股份有限公司 | Polyunsaturated fatty acid microcapsule and preparation method and application thereof |
| CN110692893A (en) * | 2019-11-12 | 2020-01-17 | 南通厚元生物科技有限公司 | Preparation method of solid beverage containing phosphatidylserine and omega-3 fatty acid |
| CN112450390A (en) * | 2020-10-30 | 2021-03-09 | 浙江波拉波拉食品股份有限公司 | Fish sausage containing DHA microcapsules and preparation method thereof |
| CN112450390B (en) * | 2020-10-30 | 2024-04-09 | 浙江波拉波拉食品股份有限公司 | Fish sausage containing DHA microcapsule and preparation method thereof |
| CN115553466A (en) * | 2022-09-30 | 2023-01-03 | 南京益唯森生物科技有限公司 | Nitraria seed oil microcapsule powder containing flax polypeptide and preparation method thereof |
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