A kind of enzymolysis cereal drink
Technical field
The present invention relates to drink, be specifically related to a kind of enzymolysis cereal drink.
Background technology
The animal food growth ratios such as the meat of taking in China resident dietary structure in the last few years, egg, newborn class are too much, too fast, and the cereal intake is downward trend gradually." Dietary transition " reflects the status of various kinds of foods in meals and the proportion that should account for, the amount of cereal is maximum, be positioned at the bottom, for Chinese, the consumption of city dweller's cereal is on the low side, function ratio is only 47 parts, is starkly lower than the zone of reasonableness of 55~65 parts.
The feed of the five cereals on traditional concept is exactly the grain of three meals in a day, and along with the quickening of people's live and work rhythm, many people's three meals in a day can not normally be taken food, and has that dietary structure is unreasonable, the collocation problem such as single, and with breakfast for very.
Grain processing is become the cereal drink, drink more conveniently, but the ubiquity raw material availability is low, and mouthfeel is bad, the defective that nutrient absorption is slower.At present, also have by enzyme solution and improve, but the general enzyme class of using is single, the product effect that obtains is common.
Summary of the invention
The invention provides the enzymolysis cereal drink of a kind of nutritious, delicate mouthfeel, good stability.
A kind of enzymolysis cereal drink comprises following component by ratio of weight and the number of copies: 2~13.5 parts of cereal, 0.66~2.42 part of complex enzyme formulation, 5~10 parts of white granulated sugars, 0.03~0.1 part of emulsifying agent, 0.1~0.26 part of thickener, 0.01~0.1 part of flavorant, 74~90 parts of pure water.
Described complex enzyme formulation is the combination of carbohydrase, amylase, protease, peptase, cellulase, lipase, and the ratio of weight and number that each enzyme preparation accounts for drink is: 0.5~2 part, carbohydrase, 0.05~0.2 part of amylase, proteinase-10 .05~0.1 part, 0.05~0.1 part of peptase, 0.005~0.01 part of cellulase, 0.005~0.01 part, lipase; Described saccharifying enzymic activity is 100000U/g, and amylase activity is 5000U/g, and prolease activity is 10000~70000U/g, and peptidase activity is 10000U/g, and cellulase activity is 10000~50000U/g, and lipase activity is 10000U/g.
Preferably, described cereal is selected from least a in oat, brown rice, corn, soya bean, Chinese sorghum, rice, yellow millet.
Preferably, described emulsifying agent is at least a in glycerin monostearate, polyglyceryl fatty acid ester, sodium pyrophosphate, glycerin monostearate; Described thickener is at least a in sodium alginate, agar, calgon, xanthans, gellan gum.
It also comprises 3~7 parts of kernels by ratio of weight and the number of copies, and described kernel is at least a in walnut kernel, shelled peanut, white sesameseed.
preferably, a kind of enzymolysis cereal drink, formed by following component by ratio of weight and the number of copies: 3~5 parts of oats, 1~2 part, brown rice, 2~4 parts of walnut kernel, 1~2 part of shelled peanut, 0.5~2 part, carbohydrase, 0.05~0.2 part of amylase, proteinase-10 .05~0.1 part, 0.05~0.1 part of peptase, 0.005~0.01 part of cellulase, 0.005~0.01 part, lipase, 5~10 parts of white granulated sugars, 0.03~0.1 part of glycerin monostearate, 0.05~0.1 part of sodium alginate, 0.05~0.1 part, agar, 0.01~0.03 part of calgon, 0.01~0.05 part of flavorant, 74.2~87.19 parts of pure water.
More preferably, a kind of enzymolysis cereal drink is comprised of following component by ratio of weight and the number of copies: 4 parts of oats, 1.5 parts, brown rice, 3.5 parts of walnut kernel, 1.5 parts of shelled peanuts, 1.4 parts, carbohydrase, 0.14 part of amylase, proteinase-10 .09 part, 0.09 part of peptase, 0.009 part of cellulase, 0.009 part, lipase, 7 parts of white granulated sugars, 0.05 part of glycerin monostearate, 0.06 part of sodium alginate, 0.07 part, agar, 0.02 part of calgon, 0.03 part of flavorant, 80 parts of pure water.
preferably, a kind of enzymolysis cereal drink, formed by following component by ratio of weight and the number of copies: 2~4 parts, brown rice, 3~5 parts of shelled peanuts, 1~2 part of white sesameseed, 0.5~2 part, carbohydrase, 0.05~0.2 part of amylase, proteinase-10 .05~0.1 part, 0.05~0.1 part of peptase, 0.005~0.01 part of cellulase, 0.005~0.01 part, lipase, 5~9 parts of white granulated sugars, 0.05~0.1 part of glycerin monostearate, 0.05~0.1 part of sodium alginate, 0.05~0.1 part, agar, 0.02~0.06 part of calgon, 0.02~0.1 part of flavorant, 77.12~88.15 parts of pure water.
More preferably, a kind of enzymolysis cereal drink is comprised of following component by ratio of weight and the number of copies: 2.4 parts, brown rice, 3.2 parts of shelled peanuts, 1.2 parts of white sesameseeds, 1 part, carbohydrase, 0.11 part of amylase, proteinase-10 .06 part, 0.07 part of peptase, 0.008 part of cellulase, 0.008 part, lipase, 7 parts of white granulated sugars, 0.07 part of glycerin monostearate, 0.07 part of sodium alginate, 0.07 part, agar, 0.04 part of calgon, 0.06 part of flavorant, 83 parts of pure water.
a kind of enzymolysis cereal drink, formed by following component by ratio of weight and the number of copies: 1~3 part of oat, 1~3 part of corn, 1~3 part of soya bean, 0.5~1 part of Chinese sorghum, 0.5~1.5 part, rice, 0.5~1 part of yellow millet, 0.5~2 part, carbohydrase, 0.05~0.2 part of amylase, proteinase-10 .05~0.1 part, 0.05~0.1 part of peptase, 0.005~0.01 part of cellulase, 0.005~0.01 part, lipase, 6~10 parts of white granulated sugars, 0.02~0.06 part of polyglyceryl fatty acid ester, 0.02~0.06 part of sodium pyrophosphate, 0.05~0.1 part of glycerin monostearate, 0.05~0.1 part of xanthans, 0.02~0.06 part of gellan gum, 0.1~0.5 part of malt extract, 0.1~0.4 part of maltodextrin, 0.01~0.05 part of salt, 0.02~0.06 part of konjaku flour, 0.02~0.05 part of sodium acid carbonate, 0.02~0.05 part, D-araboascorbic acid sodium, 0.02~0.09 part of flavorant, 72.44~88.37 parts of pure water.
preferably, a kind of enzymolysis cereal drink, formed by following component by ratio of weight and the number of copies: 1.5 parts of oats, 1.5 parts of corns, 2 parts of soya beans, 0.7 part of Chinese sorghum, 0.8 part, rice, 0.6 part of yellow millet, 1.2 parts, carbohydrase, 0.12 part of amylase, proteinase-10 .07 part, 0.07 part of peptase, 0.008 part of cellulase, 0.008 part, lipase, 7 parts of white granulated sugars, 0.04 part of polyglyceryl fatty acid ester, 0.03 part of sodium pyrophosphate, 0.07 part of glycerin monostearate, 0.06 part of xanthans, 0.04 part of gellan gum, 0.25 part of malt extract, 0.15 part of maltodextrin, 0.03 part of salt, 0.04 part of konjaku flour, 0.02 part of sodium acid carbonate, 0.03 part, D-araboascorbic acid sodium, 0.06 part of flavorant, 84 parts of pure water.
In the foregoing invention technical scheme, the effect of part component is as follows:
Amylase: hydrolyzed starch, glycogen, gelatinization and the liquefaction of help raw material, the strainability of raising raw material; Starch, glycogen are changed into monose as sweet substance and the energy that can directly absorb.
Carbohydrase: improve the recovery rate of finished product and the strainability of finished product, improve the stability of finished product; Starch is changed into monose as sweet substance and the energy that can directly absorb.
Protease: aminosal, improve the strainability of cereal enzymolysis liquid, improve the stability of finished product.
Peptase: improve the strainability of cereal enzymolysis liquid, improve the stability of finished product.
Cellulase: decomposition of cellulose, the utilization rate of raising raw material.
Lipase: i.e. Lipase, catalysis natural substrate grease hydrolysis, hydrolysate is aliphatic acid, glycerine and monoglyceride or diglyceride, improves product special flavour.
Oat is rich in the elements such as protein, starch, water-soluble dietary fiber and phosphorus, iron, calcium.B family vitamin in oat, vitamin E, niacin, folic acid, pantothenic acid are all abundanter.Also contain in addition the saponin that all lacks in cereal grain in oatmeal.The amino acid ratio of components of protein is more comprehensive, contains 8 seed amino acids of needed by human.Have beneficial liver and the effects such as stomach, beauty care.
In brown rice, rice bran and plumule partly contain abundant B family vitamin and vitamin E, can improve immune function of human body, stimulate circulation.In addition, in brown rice, the micronutrient levels such as potassium, magnesium, zinc, iron, manganese are higher, are conducive to angiocardiopathy preventing and anemia.It has also kept a large amount of dietary fibers, can promote intestinal beneficial bacterium propagation, accelerates intestines peristalsis, softening ight soil, Constipation and intestinal cancer.
Corn claims again fruit corn, be rich in vitamin A, B family vitamin, vitamin C, vitamin E, Organic Selenium, lutern, zeaxanthin, mineral matter, free amino acid, dietary fiber etc., the total reducing sugar amount is greater than 33%, sugar is mainly D~glucose, fructose, compound sugar etc., more easily be absorbed by the body and can be converted to fat, therefore being difficult for making the people to increase weight.
The sweet property of soya bean flavor is flat, have invigorating the spleen wide in, the effect of moisturizing the water that disappears, clearing heat and detoxicating, beneficial gas; Be rich in the inorganic salts such as protein, linoleic acid, B family vitamin, amino acid and calcium, phosphorus, iron.
The Chinese sorghum flavor is sweet warm in nature, is rich in calcium, phosphorus, Cobastab
1, Cobastab
6, pantothenic acid, nicotinic acid, biotin.The content of riboflavin, carrotene is also abundanter, has and the functions such as stomach, invigorating the spleen, antidiarrheal.
The rice nature and flavor are sweet flat, contain the multiple nutrients materials such as carbohydrate, protein, calcium, phosphorus, iron, vitamin B1, amino acid.Tonifying middle-Jiao and Qi, strengthening spleen and nourishing stomach and the five internal organs, promoting blood circulation, hearing-improing and eyesight improving arranged, only be tired of, quench the thirst, the effect of antidiarrheal.
Yellow millet is rich in protein, carbohydrate, carrotene, Cobastab
1, strengthening the spleen and stomach and middle kidney-nourishing, except thermal detoxification, nourishing yin and nourishing blood.
Walnut kernel is a kind of important plant protein resource, and fatty main component is the linoleic acid glyceride, and cholesterol is raise, and can also reduce enteron aisle to the absorption of cholesterol, therefore, can be used as hypertension, arteriosclerotic's invigorant.Trace element zinc and manganese contained in walnut are important component pituitary, and normal food is of value to the nutritional supplementation of brain, and brain tonic and intelligence development, face nursing and life prolonging effect are arranged.
Shelled peanut is a kind of important plant protein resource, has very high nutritive value, includes abundant fat and protein, and contains the multivitamins such as thiamine, riboflavin, niacin.Content of mineral substances is also very abundant, particularly contains the necessary amino acid of human body, and the promotion brain cell development is arranged, and strengthens the function of memory.
Glycerin monostearate, polyglyceryl fatty acid ester, sodium pyrophosphate, glycerin monostearate are emulsifying agent, promote that drink becomes stabilized emulsion, and drink is maintained a relatively stable state in a long time.
Sodium alginate, agar, calgon, xanthans and gellan gum are thickener, can improve the stickiness of drink, give the glutinous profit of food, suitable mouthfeel, and have emulsification, stable or make the effect that is suspended state concurrently.
After adopting technique scheme, the present invention compares with existing background technology, has following advantage:
Add complex enzyme formulation, make various nutritional labelings be hydrolyzed to the material that human body can better absorb, can obtain larger material nutrient component recovery rate, raising raw material comprehensive utilization ratio, fine and smooth goodization of products taste.The good stability of drink does not precipitate, without lamination, long shelf-life.
The specific embodiment
Some embodiments of the present invention below are provided, understand the present invention to help further, but protection scope of the present invention are not limited in these embodiment.
Embodiment one
A kind of enzymolysis cereal drink is comprised of following component by ratio of weight and the number of copies: 4 parts of oats, 1.5 parts, brown rice, 3.5 parts of walnut kernel, 1.5 parts of shelled peanuts, 1.4 parts, carbohydrase, 0.14 part of amylase, proteinase-10 .09 part, 0.09 part of peptase, 0.009 part of cellulase, 0.009 part, lipase, 7 parts of white granulated sugars, 0.05 part of glycerin monostearate, 0.06 part of sodium alginate, 0.07 part, agar, 0.02 part of calgon, 0.03 part of flavorant, 80 parts of pure water.
Saccharifying enzymic activity is 100000U/g, and amylase activity is 5000U/g, and prolease activity is 20000U/g, and peptidase activity is 10000U/g, and cellulase activity is 30000U/g, and lipase activity is 10000U/g.
The preparation method of above-mentioned enzymolysis cereal drink, carry out according to the following steps:
(1) raw material is processed
1. after adding the pure water gelatinization 30min of 75 ℃ after oat, brown rice grinds, cross the twice colloid mill, be ground to uniform slurries.
2. walnut kernel and shelled peanut add the rear twice colloid mill of crossing of pure water stirring of 75 ℃, are ground to uniform slurries.
1. step mixes with the slurries that 2. step obtains.
3. preliminary enzymolysis, it is 5 that mixed slurries are regulated pH value, complex enzyme formulation is added in mixed slurries and mixes, in the situation that intermittent stirring keeps 50 ℃, 30min.
4. later stage enzymolysis, in the situation that intermittent stirring keeps 63 ℃, 120min.
5. inactivator preparation is in the situation that intermittent stirring keeps 95 ℃ or fluidized state 10min.
6. filter, use screen filtration, juice removes slag to get.
(2) batch operation
1. add the pure water of 90 ℃ in changing sugar bowl, then will account in pouring into of the white granulated sugar gradation sugar bowl of white granulated sugar gross weight 90% and with adjustment to 80 ℃, dissolving rear use 300 purpose screen packs fully until white granulated sugar, filter-to obtain liquid glucose standby;
2. add the pure water of 80 ℃ to carry out the emulsification dissolving after mono stearate glyceryl ester, sodium alginate and agar and remaining white granulated sugar being mixed in emulsion tank, standby;
3. calgon is filtered after with the dissolved in purified water of 80 ℃, standby;
4. the raw material after processing and above-mentioned steps 1., 2., the feed liquid that 3. obtains is evacuated to respectively in blend tank, stir after adding flavoring essence, pump it to after stirring and add the pure water constant volume in the constant volume tank, then opening mixer stirs, its physical and chemical index is surveyed in sampling after it is even, is heated to 75 ℃ if physical and chemical index is qualified;
(3) homogeneous
Material after heating is carried out the twice homogeneous under 25MPa;
(4) adopt full automatic UHT sterilizer to carry out ultra high temperature short time sterilization, sterilising temp is 137 ℃, and the time is 30s, then adopts the full-automatic sterile bottle placer to carry out aseptic filling and sealing, is applicable to the tetra pak package dress.
Table 1 embodiment one drink nutrient component meter
Embodiment two
A kind of enzymolysis cereal drink is comprised of following component by ratio of weight and the number of copies: 5 parts of oats, 2 parts, brown rice, 2 parts of walnut kernel, 1 part of shelled peanut, 2 parts, carbohydrase, 0.2 part of amylase, proteinase-10 .05 part, 0.06 part of peptase, 0.01 part of cellulase, 0.005 part, lipase, 5 parts of white granulated sugars, 0.1 part of glycerin monostearate, 0.08 part of sodium alginate, 0.05 part, agar, 0.01 part of calgon, 0.05 part of flavorant, 85 parts of pure water.
Each enzyme activity of the present embodiment is with embodiment one.
The preparation method of the present embodiment is with embodiment one.
Embodiment three
A kind of enzymolysis cereal drink is comprised of following component by ratio of weight and the number of copies: 2.4 parts, brown rice, 3.2 parts of shelled peanuts, 1.2 parts of white sesameseeds, 1 part, carbohydrase, 0.11 part of amylase, proteinase-10 .06 part, 0.07 part of peptase, 0.008 part of cellulase, 0.008 part, lipase, 7 parts of white granulated sugars, 0.07 part of glycerin monostearate, 0.07 part of sodium alginate, 0.07 part, agar, 0.04 part of calgon, 0.06 part of flavorant, 83 parts of pure water.
Saccharifying enzymic activity is 100000U/g, and amylase activity is 5000U/g, and prolease activity is 5000U/g, and peptidase activity is 10000U/g, and cellulase activity is 10000U/g, and lipase activity is 10000U/g.
The preparation method of above-mentioned enzymolysis cereal drink, carry out according to the following steps:
(1) raw material is processed
1. after adding the pure water gelatinization 30min of 80 ℃ after the brown rice grinds, cross the twice colloid mill, be ground to uniform slurries.
2. the white sesameseed after shelled peanut and baking adds the pure water of 80 ℃ and crosses the twice colloid mill, is ground to uniform slurries.
1. step mixes with the slurries that 2. step obtains.
3. preliminary enzymolysis, it is 4 that mixed slurries are regulated pH value, complex enzyme formulation is added in mixed slurries and mixes, in the situation that intermittent stirring keeps 50 ℃, 30min.
4. later stage enzymolysis, in the situation that intermittent stirring keeps 63 ℃, 120min.
5. inactivator preparation is in the situation that intermittent stirring keeps 95 ℃ or fluidized state 10min.
6. filter, use screen filtration, juice removes slag to get.
(2) batch operation
1. add the pure water of 85 ℃ in changing sugar bowl, then will account in pouring into of the white granulated sugar gradation sugar bowl of white granulated sugar gross weight 90~95% and with adjustment to 75 ℃, dissolving rear use 300 purpose screen packs fully until white granulated sugar, filter-to obtain liquid glucose standby;
2. add the pure water of 80 ℃ to carry out the emulsification dissolving after mono stearate glyceryl ester, sodium alginate and agar and remaining white granulated sugar being mixed in emulsion tank, standby;
3. calgon is filtered after with the hot dissolved in purified water of 75 ℃, standby;
4. the raw material after processing and above-mentioned steps 1., 2., the feed liquid that 3. obtains is evacuated to respectively in blend tank, stir after adding flavoring essence, pump it to after stirring and add the pure water constant volume in the constant volume tank, then opening mixer stirs, its physical and chemical index is surveyed in sampling after it is even, is heated to 75 ℃ if physical and chemical index is qualified;
(3) homogeneous
Material after heating is carried out the twice homogeneous under 20MPa;
(4) adopt automatic filling machine to carry out can and sealing, before can, the temperature of material remains on 80 ℃, adopts horizontal sterilizer to carry out sterilization after can, regulate sterilizing time and temperature according to different size, if specification is 240ml, sterilization temperature is 121 ℃, and sterilizing time is 25min; If specification is 500ml, sterilization temperature is 121 ℃, and sterilizing time is 30min.Lower the temperature with circulating water after sterilization, need within 30min, product is being cooled to below 40 ℃, be applicable to built-up tin, packed packing.
Table 2 embodiment three drink nutrient component meters
Embodiment four
A kind of enzymolysis cereal drink is comprised of following component by ratio of weight and the number of copies: 4 parts, brown rice, 3 parts of shelled peanuts, 1 part of white sesameseed, 1.5 parts, carbohydrase, 0.2 part of amylase, proteinase-10 .1 part, 0.1 part of peptase, 0.005 part of cellulase, 0.01 part, lipase, 5 parts of white granulated sugars, 0.1 part of glycerin monostearate, 0.05 part of sodium alginate, 0.05 part, agar, 0.06 part of calgon, 0.03 part of flavorant, 87 parts of pure water.
Each enzyme activity of the present embodiment is with embodiment three.
The preparation method of the present embodiment is with embodiment three.
Embodiment five
1.5 parts of oats, 1.5 parts of corns, 2 parts of soya beans, 0.7 part of Chinese sorghum, 0.8 part, rice, 0.6 part of yellow millet, 1.2 parts, carbohydrase, 0.12 part of amylase, proteinase-10 .07 part, 0.07 part of peptase, 0.008 part of cellulase, 0.008 part, lipase, 7 parts of white granulated sugars, 0.04 part of polyglyceryl fatty acid ester, 0.03 part of sodium pyrophosphate, 0.07 part of glycerin monostearate, 0.06 part of xanthans, 0.04 part of gellan gum, 0.25 part of malt extract, 0.15 part of maltodextrin, 0.03 part of salt, 0.04 part of konjaku flour, 0.02 part of sodium acid carbonate, 0.03 part, D-araboascorbic acid sodium, 0.06 part of flavorant, 84 parts of pure water.
Saccharifying enzymic activity is 100000U/g, and amylase activity is 5000U/g, and prolease activity is 70000U/g, and peptidase activity is 10000U/g, and cellulase activity is 50000U/g, and lipase activity is 10000U/g.
The preparation method of above-mentioned enzymolysis cereal drink, carry out according to the following steps:
(1) raw material is processed
1. after Chinese sorghum, rice grinds after the hot pure water gelatinization 30min more than 70 ℃, cross the twice colloid mill, be ground to uniform slurries.
2. corn, soya bean, yellow millet are crossed the twice colloid mill together with the oat after baking after boiling 30min, are ground to uniform slurries.
1. step mixes with the slurries that 2. step obtains.
3. preliminary enzymolysis, it is 6 that mixed slurries are regulated pH value, complex enzyme formulation is added in mixed slurries and mixes, in the situation that intermittent stirring keeps 50 ℃, 30min.
4. later stage enzymolysis, in the situation that intermittent stirring keeps 63 ℃, 120min.
5. inactivator preparation is in the situation that intermittent stirring keeps 95 ℃ or fluidized state 10min.
6. filter, use screen filtration, juice removes slag to get.
(2) batch operation
1. add the pure water more than 85 ℃ in changing sugar bowl, then will account in pouring into of the white granulated sugar gradation sugar bowl of white granulated sugar gross weight 90~95% and with adjustment to 70~85 ℃, dissolving rear use 300 purpose screen packs fully until white granulated sugar, filter-to obtain liquid glucose standby;
2. add the pure water more than 75 ℃ to carry out the emulsification dissolving after mono stearate glyceryl ester, xanthans, polyglyceryl fatty acid ester, konjac glucomannan and gellan gum and remaining white granulated sugar being mixed in emulsion tank, standby.
3. with filtering after malt extract, maltodextrin, sodium pyrophosphate, sodium acid carbonate, D-araboascorbic acid sodium, the dissolved in purified water of salt with heat, standby.
4. the raw material after processing and above-mentioned steps 1., 2., the feed liquid that 3. obtains is evacuated to respectively in blend tank, stir after adding flavoring essence, pump it to after stirring and add the pure water constant volume in the constant volume tank, then opening mixer stirs, its physical and chemical index is surveyed in sampling after it is even, is heated to 75 ℃ if physical and chemical index is qualified.
(3) homogeneous
Material after heating is carried out the twice homogeneous under 20MPa;
(4) adopt full automatic UHT sterilizer to carry out ultra high temperature short time sterilization, sterilising temp is 137 ℃, and the time is 30s, then adopts the full-automatic sterile bottle placer to carry out aseptic filling and sealing, is applicable to the tetra pak package dress.
Table 3 embodiment five drink nutrient component meters
Embodiment six
1 part of oat, 3 parts of corns, 3 parts of soya beans, 1 part of Chinese sorghum, 1.5 parts, rice, 1 part of yellow millet, 2 parts, carbohydrase, 0.06 part of amylase, proteinase-10 .1 part, 0.1 part of peptase, 0.01 part of cellulase, 0.005 part, lipase, 9 parts of white granulated sugars, 0.02 part of polyglyceryl fatty acid ester, 0.03 part of sodium pyrophosphate, 0.05 part of glycerin monostearate, 0.1 part of xanthans, 0.02 part of gellan gum, 0.1 part of malt extract, 0.4 part of maltodextrin, 0.05 part of salt, 0.02 part of konjaku flour, 0.04 part of sodium acid carbonate, 0.05 part, D-araboascorbic acid sodium, 0.02 part of flavorant, 75 parts of pure water.
Each enzyme activity of the present embodiment is with embodiment five.
The preparation method of the present embodiment is with embodiment five.
Above-described embodiment is the better embodiment of the present invention; but embodiments of the present invention are not restricted to the described embodiments; other any do not deviate from change, the modification done under Spirit Essence of the present invention and principle, substitutes, combination, simplify; all should be the substitute mode of equivalence, within being included in protection scope of the present invention.