CN107518436B - A kind of DHA grease microscapsule powder and preparation method thereof containing polypeptide - Google Patents
A kind of DHA grease microscapsule powder and preparation method thereof containing polypeptide Download PDFInfo
- Publication number
- CN107518436B CN107518436B CN201710758771.0A CN201710758771A CN107518436B CN 107518436 B CN107518436 B CN 107518436B CN 201710758771 A CN201710758771 A CN 201710758771A CN 107518436 B CN107518436 B CN 107518436B
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- CN
- China
- Prior art keywords
- protein
- polypeptide
- dha
- minutes
- microscapsule powder
- Prior art date
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- 239000000843 powder Substances 0.000 title claims abstract description 67
- 229920001184 polypeptide Polymers 0.000 title claims abstract description 39
- 102000004196 processed proteins & peptides Human genes 0.000 title claims abstract description 39
- 108090000765 processed proteins & peptides Proteins 0.000 title claims abstract description 39
- 238000002360 preparation method Methods 0.000 title claims abstract description 22
- 239000004519 grease Substances 0.000 title claims abstract description 19
- 102000004169 proteins and genes Human genes 0.000 claims abstract description 36
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 36
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 26
- 239000003963 antioxidant agent Substances 0.000 claims abstract description 7
- 239000003995 emulsifying agent Substances 0.000 claims abstract description 7
- 230000003078 antioxidant effect Effects 0.000 claims abstract description 5
- 150000001720 carbohydrates Chemical class 0.000 claims abstract description 5
- 235000018102 proteins Nutrition 0.000 claims description 35
- 102000004190 Enzymes Human genes 0.000 claims description 28
- 108090000790 Enzymes Proteins 0.000 claims description 28
- 229940088598 enzyme Drugs 0.000 claims description 28
- 239000004365 Protease Substances 0.000 claims description 25
- 238000003756 stirring Methods 0.000 claims description 25
- 230000009849 deactivation Effects 0.000 claims description 17
- 235000013336 milk Nutrition 0.000 claims description 16
- 239000008267 milk Substances 0.000 claims description 16
- 210000004080 milk Anatomy 0.000 claims description 16
- 239000000203 mixture Substances 0.000 claims description 15
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 14
- 102000004407 Lactalbumin Human genes 0.000 claims description 14
- 108090000942 Lactalbumin Proteins 0.000 claims description 14
- 102000009027 Albumins Human genes 0.000 claims description 11
- 108010088751 Albumins Proteins 0.000 claims description 11
- 108010004032 Bromelains Proteins 0.000 claims description 11
- 108090000526 Papain Proteins 0.000 claims description 11
- 235000019835 bromelain Nutrition 0.000 claims description 11
- 235000019834 papain Nutrition 0.000 claims description 11
- 229940055729 papain Drugs 0.000 claims description 11
- 102000057297 Pepsin A Human genes 0.000 claims description 10
- 108090000284 Pepsin A Proteins 0.000 claims description 10
- 239000012153 distilled water Substances 0.000 claims description 10
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- 238000004321 preservation Methods 0.000 claims description 9
- 108010068370 Glutens Proteins 0.000 claims description 8
- 108010084695 Pea Proteins Proteins 0.000 claims description 8
- 101710180319 Protease 1 Proteins 0.000 claims description 8
- 101710180316 Protease 2 Proteins 0.000 claims description 8
- 101710137710 Thioesterase 1/protease 1/lysophospholipase L1 Proteins 0.000 claims description 8
- 241000209140 Triticum Species 0.000 claims description 8
- 235000021307 Triticum Nutrition 0.000 claims description 8
- 235000021312 gluten Nutrition 0.000 claims description 8
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- 108010073771 Soybean Proteins Proteins 0.000 claims description 7
- 229910052757 nitrogen Inorganic materials 0.000 claims description 7
- 235000019710 soybean protein Nutrition 0.000 claims description 7
- GVJHHUAWPYXKBD-UHFFFAOYSA-N (±)-α-Tocopherol Chemical compound OC1=C(C)C(C)=C2OC(CCCC(C)CCCC(C)CCCC(C)C)(C)CCC2=C1C GVJHHUAWPYXKBD-UHFFFAOYSA-N 0.000 claims description 6
- 229930006000 Sucrose Natural products 0.000 claims description 6
- 239000005720 sucrose Substances 0.000 claims description 6
- 235000006708 antioxidants Nutrition 0.000 claims description 5
- JLPULHDHAOZNQI-ZTIMHPMXSA-N 1-hexadecanoyl-2-(9Z,12Z-octadecadienoyl)-sn-glycero-3-phosphocholine Chemical compound CCCCCCCCCCCCCCCC(=O)OC[C@H](COP([O-])(=O)OCC[N+](C)(C)C)OC(=O)CCCCCCC\C=C/C\C=C/CCCCC JLPULHDHAOZNQI-ZTIMHPMXSA-N 0.000 claims description 4
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 claims description 4
- 244000269722 Thea sinensis Species 0.000 claims description 4
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- 150000008442 polyphenolic compounds Chemical class 0.000 claims description 4
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- 229940083466 soybean lecithin Drugs 0.000 claims description 4
- -1 sucrose fatty ester Chemical class 0.000 claims description 4
- HDTRYLNUVZCQOY-UHFFFAOYSA-N α-D-glucopyranosyl-α-D-glucopyranoside Natural products OC1C(O)C(O)C(CO)OC1OC1C(O)C(O)C(O)C(CO)O1 HDTRYLNUVZCQOY-UHFFFAOYSA-N 0.000 claims description 3
- LDVVTQMJQSCDMK-UHFFFAOYSA-N 1,3-dihydroxypropan-2-yl formate Chemical compound OCC(CO)OC=O LDVVTQMJQSCDMK-UHFFFAOYSA-N 0.000 claims description 3
- OWEGMIWEEQEYGQ-UHFFFAOYSA-N 100676-05-9 Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC2C(OC(O)C(O)C2O)CO)O1 OWEGMIWEEQEYGQ-UHFFFAOYSA-N 0.000 claims description 3
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 3
- 239000011786 L-ascorbyl-6-palmitate Substances 0.000 claims description 3
- QAQJMLQRFWZOBN-LAUBAEHRSA-N L-ascorbyl-6-palmitate Chemical compound CCCCCCCCCCCCCCCC(=O)OC[C@H](O)[C@H]1OC(=O)C(O)=C1O QAQJMLQRFWZOBN-LAUBAEHRSA-N 0.000 claims description 3
- 229920002774 Maltodextrin Polymers 0.000 claims description 3
- 239000005913 Maltodextrin Substances 0.000 claims description 3
- GUBGYTABKSRVRQ-PICCSMPSSA-N Maltose Natural products O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-PICCSMPSSA-N 0.000 claims description 3
- 108091005804 Peptidases Proteins 0.000 claims description 3
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims description 3
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 3
- HDTRYLNUVZCQOY-WSWWMNSNSA-N Trehalose Natural products O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-WSWWMNSNSA-N 0.000 claims description 3
- 229930003427 Vitamin E Natural products 0.000 claims description 3
- HDTRYLNUVZCQOY-LIZSDCNHSA-N alpha,alpha-trehalose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-LIZSDCNHSA-N 0.000 claims description 3
- 235000010385 ascorbyl palmitate Nutrition 0.000 claims description 3
- WIGCFUFOHFEKBI-UHFFFAOYSA-N gamma-tocopherol Natural products CC(C)CCCC(C)CCCC(C)CCCC1CCC2C(C)C(O)C(C)C(C)C2O1 WIGCFUFOHFEKBI-UHFFFAOYSA-N 0.000 claims description 3
- 239000008101 lactose Substances 0.000 claims description 3
- 229940035034 maltodextrin Drugs 0.000 claims description 3
- 150000003904 phospholipids Chemical class 0.000 claims description 3
- 235000019419 proteases Nutrition 0.000 claims description 3
- 235000010378 sodium ascorbate Nutrition 0.000 claims description 3
- PPASLZSBLFJQEF-RKJRWTFHSA-M sodium ascorbate Substances [Na+].OC[C@@H](O)[C@H]1OC(=O)C(O)=C1[O-] PPASLZSBLFJQEF-RKJRWTFHSA-M 0.000 claims description 3
- 229960005055 sodium ascorbate Drugs 0.000 claims description 3
- PPASLZSBLFJQEF-RXSVEWSESA-M sodium-L-ascorbate Chemical compound [Na+].OC[C@H](O)[C@H]1OC(=O)C(O)=C1[O-] PPASLZSBLFJQEF-RXSVEWSESA-M 0.000 claims description 3
- 235000019165 vitamin E Nutrition 0.000 claims description 3
- 229940046009 vitamin E Drugs 0.000 claims description 3
- 239000011709 vitamin E Substances 0.000 claims description 3
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 2
- 238000013019 agitation Methods 0.000 claims description 2
- GUBGYTABKSRVRQ-QUYVBRFLSA-N beta-maltose Chemical compound OC[C@H]1O[C@H](O[C@H]2[C@H](O)[C@@H](O)[C@H](O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@@H]1O GUBGYTABKSRVRQ-QUYVBRFLSA-N 0.000 claims description 2
- 229910052799 carbon Inorganic materials 0.000 claims description 2
- 239000008103 glucose Substances 0.000 claims description 2
- 230000001360 synchronised effect Effects 0.000 claims description 2
- 241000283690 Bos taurus Species 0.000 claims 1
- 244000068988 Glycine max Species 0.000 claims 1
- 235000010469 Glycine max Nutrition 0.000 claims 1
- 239000005862 Whey Substances 0.000 claims 1
- 102000007544 Whey Proteins Human genes 0.000 claims 1
- 108010046377 Whey Proteins Proteins 0.000 claims 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 claims 1
- 239000000463 material Substances 0.000 abstract description 10
- 235000020207 hydrolysed-protein milk formula Nutrition 0.000 abstract description 5
- 240000007594 Oryza sativa Species 0.000 abstract description 4
- 235000007164 Oryza sativa Nutrition 0.000 abstract description 4
- 238000010521 absorption reaction Methods 0.000 abstract description 4
- 230000029087 digestion Effects 0.000 abstract description 4
- 235000013312 flour Nutrition 0.000 abstract description 4
- 235000013350 formula milk Nutrition 0.000 abstract description 4
- 235000009566 rice Nutrition 0.000 abstract description 4
- 239000007787 solid Substances 0.000 abstract description 4
- 235000013361 beverage Nutrition 0.000 abstract description 3
- 239000011162 core material Substances 0.000 abstract description 3
- 238000002156 mixing Methods 0.000 abstract description 3
- 235000015895 biscuits Nutrition 0.000 abstract description 2
- 235000020124 milk-based beverage Nutrition 0.000 abstract description 2
- 235000008476 powdered milk Nutrition 0.000 abstract description 2
- 238000004945 emulsification Methods 0.000 abstract 1
- 239000012467 final product Substances 0.000 abstract 1
- 230000007065 protein hydrolysis Effects 0.000 abstract 1
- MBMBGCFOFBJSGT-KUBAVDMBSA-N all-cis-docosa-4,7,10,13,16,19-hexaenoic acid Chemical compound CC\C=C/C\C=C/C\C=C/C\C=C/C\C=C/C\C=C/CCC(O)=O MBMBGCFOFBJSGT-KUBAVDMBSA-N 0.000 description 86
- 235000020669 docosahexaenoic acid Nutrition 0.000 description 45
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- 239000003094 microcapsule Substances 0.000 description 15
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- 238000005516 engineering process Methods 0.000 description 5
- 238000000265 homogenisation Methods 0.000 description 5
- YZXBAPSDXZZRGB-DOFZRALJSA-N arachidonic acid Chemical compound CCCCC\C=C/C\C=C/C\C=C/C\C=C/CCCC(O)=O YZXBAPSDXZZRGB-DOFZRALJSA-N 0.000 description 4
- 238000013475 authorization Methods 0.000 description 4
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- 210000004885 white matter Anatomy 0.000 description 4
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- DVSZKTAMJJTWFG-SKCDLICFSA-N (2e,4e,6e,8e,10e,12e)-docosa-2,4,6,8,10,12-hexaenoic acid Chemical compound CCCCCCCCC\C=C\C=C\C=C\C=C\C=C\C=C\C(O)=O DVSZKTAMJJTWFG-SKCDLICFSA-N 0.000 description 2
- GZJLLYHBALOKEX-UHFFFAOYSA-N 6-Ketone, O18-Me-Ussuriedine Natural products CC=CCC=CCC=CCC=CCC=CCC=CCCCC(O)=O GZJLLYHBALOKEX-UHFFFAOYSA-N 0.000 description 2
- 241000195493 Cryptophyta Species 0.000 description 2
- OYHQOLUKZRVURQ-HZJYTTRNSA-N Linoleic acid Chemical compound CCCCC\C=C/C\C=C/CCCCCCCC(O)=O OYHQOLUKZRVURQ-HZJYTTRNSA-N 0.000 description 2
- JAZBEHYOTPTENJ-JLNKQSITSA-N all-cis-5,8,11,14,17-icosapentaenoic acid Chemical compound CC\C=C/C\C=C/C\C=C/C\C=C/C\C=C/CCCC(O)=O JAZBEHYOTPTENJ-JLNKQSITSA-N 0.000 description 2
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- KAUVQQXNCKESLC-UHFFFAOYSA-N docosahexaenoic acid (DHA) Natural products COC(=O)C(C)NOCC1=CC=CC=C1 KAUVQQXNCKESLC-UHFFFAOYSA-N 0.000 description 2
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- 150000004676 glycans Chemical class 0.000 description 2
- 235000020778 linoleic acid Nutrition 0.000 description 2
- OYHQOLUKZRVURQ-IXWMQOLASA-N linoleic acid Natural products CCCCC\C=C/C\C=C\CCCCCCCC(O)=O OYHQOLUKZRVURQ-IXWMQOLASA-N 0.000 description 2
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- YUFFSWGQGVEMMI-JLNKQSITSA-N (7Z,10Z,13Z,16Z,19Z)-docosapentaenoic acid Chemical compound CC\C=C/C\C=C/C\C=C/C\C=C/C\C=C/CCCCCC(O)=O YUFFSWGQGVEMMI-JLNKQSITSA-N 0.000 description 1
- 235000021294 Docosapentaenoic acid Nutrition 0.000 description 1
- 229920000881 Modified starch Polymers 0.000 description 1
- 239000004368 Modified starch Substances 0.000 description 1
- 108010009736 Protein Hydrolysates Proteins 0.000 description 1
- 235000010489 acacia gum Nutrition 0.000 description 1
- 239000001785 acacia senegal l. willd gum Substances 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 235000020661 alpha-linolenic acid Nutrition 0.000 description 1
- 230000001458 anti-acid effect Effects 0.000 description 1
- 230000003110 anti-inflammatory effect Effects 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- SQVRNKJHWKZAKO-UHFFFAOYSA-N beta-N-Acetyl-D-neuraminic acid Natural products CC(=O)NC1C(O)CC(O)(C(O)=O)OC1C(O)C(O)CO SQVRNKJHWKZAKO-UHFFFAOYSA-N 0.000 description 1
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- 125000002496 methyl group Chemical group [H]C([H])([H])* 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23P—SHAPING OR WORKING OF FOODSTUFFS, NOT FULLY COVERED BY A SINGLE OTHER SUBCLASS
- A23P10/00—Shaping or working of foodstuffs characterised by the products
- A23P10/30—Encapsulation of particles, e.g. foodstuff additives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
- A23C9/00—Milk preparations; Milk powder or milk powder preparations
- A23C9/152—Milk preparations; Milk powder or milk powder preparations containing additives
- A23C9/1526—Amino acids; Peptides; Protein hydrolysates; Nucleic acids; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J3/00—Working-up of proteins for foodstuffs
- A23J3/30—Working-up of proteins for foodstuffs by hydrolysis
- A23J3/32—Working-up of proteins for foodstuffs by hydrolysis using chemical agents
- A23J3/34—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes
- A23J3/341—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes of animal proteins
- A23J3/343—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes of animal proteins of dairy proteins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J3/00—Working-up of proteins for foodstuffs
- A23J3/30—Working-up of proteins for foodstuffs by hydrolysis
- A23J3/32—Working-up of proteins for foodstuffs by hydrolysis using chemical agents
- A23J3/34—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes
- A23J3/346—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes of vegetable proteins
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/385—Concentrates of non-alcoholic beverages
- A23L2/39—Dry compositions
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/52—Adding ingredients
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/115—Fatty acids or derivatives thereof; Fats or oils
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/115—Fatty acids or derivatives thereof; Fats or oils
- A23L33/12—Fatty acids or derivatives thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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Abstract
The present invention provides a kind of DHA grease microscapsule powder and preparation method thereof containing polypeptide.The microscapsule powder includes each component of following weight percentage: polypeptide 20.3-41.2%, protein 8.8-29.7%, carbohydrate 8.8-28.8%, DHA algal oil 20-40%, emulsifier 1%, antioxidant 0.2%.The preparation method is that obtaining the mixing wall material of albumen and polypeptide by moderate control protein hydrolysis degree, then the emulsification of wall material, core material, emulsifier and antioxidant is mixed, is high-pressure homogeneous, be finally spray-dried to obtain the final product.Polypeptide is rich in DHA microscapsule powder wall material prepared by the present invention, it is easier to the digestion and absorption of human body, it can be applied not only in the formula such as ordinary powdered milk, rice flour, biscuit, milk beverage, it can also be applied in protein hydrolysate formula, it more can directly take after mixing it with water as solid beverage, particularly suitable children and mid-aged population are edible.
Description
Technical field
The present invention relates to a kind of DHA grease microscapsule powder and preparation method thereof containing polypeptide, belongs to food processing
Technical field.
Background technique
Polyunsaturated fatty acid refers to containing two or more double bonds, and carbon chain lengths are the straight of 18-22 carbon atom
Chain fatty acid.According to the position of first double-linked carbon in methyl end, polyunsaturated fatty acid can be divided into ω -3 and ω -6 series
Polyunsaturated fatty acid.ω -3: alpha-linolenic acid (ALA), eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA)；ω-
6: linoleic acid (LA), gamma-Linolenic acid (GLA) and arachidonic acid (ARA).
Docosahexaenoic acid (DHA) belongs to a kind of important polyunsaturated fatty acid of ω -3 series, is brain and view
The important composition ingredient of nethike embrane, is commonly called as " docosapentaenoic acid ", and can maintain and promote nerve growth.DHA is in human brain cortex
Middle content is up to 20%, proportion about 50% in eye retina, most important to tire infant intelligence and visual acuity.Through
Cross the scientific validation of many years, it is determined that effect of DHA in terms of brain tonic and intelligence development, softening blood vessel, anti inflammatory immunity, inhibition,
Therefore, in the diet of people, a certain amount of DHA is rationally added into diet and has been subjected to widely paying close attention to and paying attention to,
Thus nutritive value that DHA has and commercial significance are increasingly significant.
DHA is applied in baby milk powder primarily as new nutrition fortifier in microcapsule embedded mode at present.
Microcapsules technology be it is a kind of solid or liquid are embedded with filmogen, thus protect core material from adverse environmental factors such as
Temperature, humidity, oxygen etc. influence, and to improve the stability and shelf life of encapsulation object, extend a kind of control of encapsulation object application range
Release technology.Unsaturated fatty acid is coated using microcapsules technology, to improve the stability of grease, makes its nutritive value
It maximizes the retention, has broad application prospects with flavor.
There are two types of the existing main wall materials of DHA microscapsule powder, and one kind is protein, and another kind is polysaccharide, such as patent public affairs
Cloth number is that CN102228257A algae oil DHA microcapsules and preparation method thereof, Patent publication No CN104906071A are with DHA grease
A kind of microcapsules containing sialic acid and DHA of the production technology of waste DHA microcapsules, patent authorization number CN104041827B
And preparation method thereof, Patent publication No CN104621570A high stability algae oil DHA microscapsule powder and preparation method thereof etc.,
Wall material is modified starch and Arabic gum；Such as a kind of anti-acid microalgae DHA grease microcapsules of patent authorization number CN104432059B
A kind of DHA microcapsules of powder, patent authorization number CN101884415B and its preparation process, wall material are mainly protein；Also
Be to be mixed as wall material, such as a kind of high fuel load of patent authorization number CN103549442B using protein and carbohydrate
The preparation process of DHA algal oil microcapsule powder and its preparation process, Patent publication No CN101878903A new DHA microcapsules.This
It can be added in normal milk powder or rice flour using protein or polysaccharide as the DHA microcapsules of wall material a bit, for digestion and absorption function
Normal crowd is edible, but the infant, old that, digestion and absorption function unsound crowd such as albumen poor for constitution does not tolerate
Year people, major operation patient etc., normal milk powder or rice flour are simultaneously not suitable for, and at this moment the milk powder of protein hydrolysate formula is exactly optimal choosing
It selects, and the DHA microscapsule powder of traditional wall material cannot be applied to well in protein hydrolysate formula milk.
In order to meet the needs of protein hydrolysate formula milk market, the present invention is made by carrying out appropriate enzymatic hydrolysis to protein
A kind of its mixture for becoming polypeptide and albumen, then be used to embed DHA grease, to allow to be applied to protein hydrolysate
In formula milk；Product of the present invention can also eat for children, mid-aged population, widen directly as solid beverage simultaneously
The application range of DHA microscapsule powder.
Summary of the invention
In view of the deficiencies of the prior art, the present invention provides a kind of DHA grease microcapsule powder powder formulation containing polypeptide
And preparation method thereof.The present invention is using protein and polypeptide as microcapsule wall material, using DHA algal oil as core material, then adds certain
The emulsifier and antioxidant of amount are made by special process process.
The purpose of the present invention is what is be achieved through the following technical solutions:
In a first aspect, the present invention provides a kind of DHA grease microscapsule powder containing polypeptide, including following quality hundred
Divide each component of content:
The too high levels of the polypeptide can then reduce embedding effect.
Preferably, the protein is milk albumin, in sheep lactalbumin, soybean protein, wheat gluten, pea protein
Any one or a few.
Preferably, the polypeptide is that protein is made by protease hydrolytic；The protein be milk albumin,
Sheep lactalbumin, soybean protein, wheat gluten, any one or a few in pea protein；The protease be trypsase,
One or more of pepsin, papain, bromelain.
Preferably, when the protein is milk albumin and/or sheep lactalbumin, with trypsase and papain
It is combined hydrolysis；When the protein is at least one of soybean protein, wheat gluten, pea protein, pepsin is used
Hydrolysis is combined with bromelain.
Preferably, the carbohydrate is lactose, maltodextrin, maltose, sucrose, trehalose, one in glucose
Kind or several mixtures.
Preferably, the emulsifier is one of monoglyceride, soybean lecithin, modified phospholipid, sucrose fatty ester or several
The mixture of kind example；The antioxidant is one of tea polyphenols, ascorbyl palmitate, sodium ascorbate, vitamin E
Or several mixture.
Second aspect, the preparation method of the present invention provides a kind of DHA grease microscapsule powder containing polypeptide, including
Following steps:
A, protein is not exclusively digested, prepares the mixture of protein and polypeptide；
B, carbohydrate, emulsifier are added in the mixture of protein made from step A and polypeptide, are stirred
Uniformly, and at 70~80 DEG C 20~30 minutes are kept the temperature, obtains water phase；
C, it adds antioxidants in DHA algal oil and stirs evenly, in the closed container of inflated with nitrogen, be added to 60~80
30~60 minutes are kept the temperature at DEG C, obtains oily phase；
D, under agitation, oil is mutually squeezed into water phase, is sheared 3~5 minutes with cutter, is synchronized and be cooled to 60 DEG C,
Then it homogeneous 2~3 times under the conditions of pressure is 25~45MPa, then is spray-dried up to the DHA microscapsule powder.
Preferably, in step A, when the protein is milk albumin and/or sheep lactalbumin, specific preparation method is such as
Under:
A1, protein is weighed, 2~4 times of distilled water is added to stir and is heated to 37 DEG C, dissolve albumen sufficiently；
Protease 1 is added in A2, the solution made from step A1, hydrolyzes 5~15 minutes, is then heated to 80 DEG C, heat preservation
Enzyme deactivation in 20 minutes；
A3, by step A2, treated that solution is cooled to 55~60 DEG C, adds protease 2, hydrolyzes 25~30 minutes, so
After be heated to 80 DEG C, keep the temperature enzyme deactivation in 20 minutes；Up to the mixture of the protein and polypeptide；
The protease 1 is trypsase or pepsin；Protease 2 is papain or bromelain.
Preferably, when the protein is milk albumin and/or sheep lactalbumin, protease 1 is trypsase, and enzyme adds
Entering concentration is 4000~8000U/g；Protease 2 is papain, and it is 5000~10000U/g that concentration, which is added, in enzyme.
Preferably, when the protein is at least one of soybean protein, wheat gluten, pea protein, protease 1 is
Pepsin, it is 2000~4000U/g that concentration, which is added, in enzyme；Protease 2 be bromelain, enzyme be added concentration be 4000~
8000U/g。
Preferably, when the protein is at least one of soybean protein, wheat gluten, pea protein, in step A1,
The pH value of solution need to be adjusted to 2.
The prior art is compared, the present invention have it is following the utility model has the advantages that
1, zymotechnic is applied in the microcapsule embedded technical field of DHA by the present invention for the first time.
2, polypeptide is rich in DHA microscapsule powder bar prepared by the present invention, it is easier to the digestion and absorption of human body, no
It can be applied only in the formula such as ordinary powdered milk, rice flour, biscuit, milk beverage, can also be applied in protein hydrolysate formula, more may be used
It is taken after mixing it with water with directly regarding solid beverage, has widened the application range of DHA, particularly suitable children and mid-aged population are edible.
3, the present invention is by controlled enzymatic hydrolysis degree, so that polypeptide content is 20.3-41.2%, protein content is
8.8-29.7%, to keep the microcapsule embedded effect of DHA more preferable, application is wider.
Specific embodiment
The present invention is described in detail combined with specific embodiments below.Following embodiment will be helpful to the technology of this field
Personnel further understand the present invention, but the invention is not limited in any way.It should be pointed out that the ordinary skill of this field
For personnel, without departing from the inventive concept of the premise, various modifications and improvements can be made.These belong to the present invention
Protection scope.
Embodiment 1
(1) 5kg milk albumin is weighed, the distilled water of 10kg is added to stir and is heated to 37 DEG C, keeps lactalbumin sufficiently molten
The trypsase of 20,000,000 units is added in solution, hydrolyzes 5 minutes, mixed liquor is heated to 80 DEG C later, keeps the temperature enzyme deactivation in 20 minutes；
Fluid temperature is down to 60 DEG C again, the papain of 25,000,000 units is added, hydrolyzes 30 minutes, is later heated to mixed liquor
80 DEG C, keep the temperature enzyme deactivation in 20 minutes.
(2) weigh the lactose of 0.88kg, 0.1kg monoglyceride is added in (1), is stirred evenly and in 70 DEG C, keep the temperature 20 minutes,
This is water phase.
(3) tea polyphenols for weighing 0.02kg, which are added in the DHA algal oil of 4kg, to stir evenly, in the closed container for rushing nitrogen,
60 DEG C are heated to, keeps the temperature 30 minutes, this is oily phase.
(4) oil is mutually squeezed into water phase under stirring conditions, is sheared 5 minutes with cutter, synchronizes and be cooled to 60 DEG C, it
It afterwards homogeneous 3 times under the conditions of homogenization pressure is 45MP, is finally spray-dried and 8.6kgDHA microscapsule powder is made, through detecting albumen
The gross mass that matter accounts for microscapsule powder total weight is 29.7%, and polypeptide (molecular weight≤10000) accounts for microscapsule powder gross weight
The 20.3% of amount, surface oil content 0.1%, powder color are milky.62 DEG C of destructive testings, peroxide value after the 45th day
2.1meq/kg, powder color slightly turn yellow than before, are faint yellow.
Embodiment 2
(1) the sheep lactalbumin of 5kg is weighed, the distilled water of 20kg stirs and is heated to 37 DEG C, keeps lactalbumin sufficiently molten
Solution is first added the trypsase of 40,000,000 units, 15 minutes, mixed liquor is heated to 80 DEG C later, keeps the temperature enzyme deactivation in 20 minutes；Again
Fluid temperature is down to 60 DEG C, the papain of 50,000,000 units is added, hydrolyzes 30 minutes, mixed liquor is heated to 80 later
DEG C, keep the temperature enzyme deactivation in 20 minutes.
(2) weigh the maltodextrin of 2.88kg, 0.1kg soybean lecithin is added in (1), stir evenly and in 80 DEG C, heat preservation
30 minutes, this was water phase.
(3) ascorbyl palmitate for weighing 0.02kg, which is added in the DHA algal oil of 2kg, to stir evenly, and is rushing nitrogen
In closed container, 80 DEG C are heated to, keeps the temperature 60m minutes, this is oily phase.
(4) oil is mutually squeezed into water phase under stirring conditions, is sheared 3 minutes with cutter, synchronizes and be cooled to 60 DEG C, it
Afterwards homogeneous 2 times under the conditions of homogenization pressure is 25MP, it is finally spray-dried the DHA microscapsule powder that 8.5kg is made, through detecting egg
The gross mass that white matter accounts for microscapsule powder total weight is 9.9%, and polypeptide (molecular weight≤10000) accounts for microscapsule powder gross weight
The 40.1% of amount, surface oil 0.56%, powder color are milky.62 DEG C of destructive testings, peroxide value after the 45th day
3.6meq/kg, powder color slightly turn yellow than before, are faint yellow.
Embodiment 3
(1) soybean protein for weighing 5kg adds the distilled water of 10kg to stir and is heated to 37 DEG C, and adjusting PH to 2 is added
The pepsin of 10000000 units hydrolyzes 5 minutes, mixed liquor is heated to 80 DEG C later, keeps the temperature enzyme deactivation in 20 minutes；Again by liquid
Temperature is down to 55 DEG C, and the bromelain of 20,000,000 units is added, and hydrolyzes 25 minutes, mixed liquor is heated to 80 DEG C later, protect
Warm enzyme deactivation in 20 minutes.
(2) modified phospholipid of the maltose, 0.1kg that weigh 0.88kg is added in (1), is stirred evenly and in 70 DEG C, heat preservation
20 minutes, this was water phase.
(3) sodium ascorbate for weighing 0.02kg, which is added in the DHA algal oil of 4kg, to stir evenly, in the closed appearance for rushing nitrogen
In device, 80 DEG C are heated to, keeps the temperature 60 minutes, this is oily phase.
(4) oil is mutually squeezed into water phase under stirring conditions, is sheared 5 minutes with cutter, synchronizes and be cooled to 60 DEG C, it
Afterwards homogeneous 3 times under the conditions of homogenization pressure is 45MP, it is finally spray-dried the DHA microscapsule powder that 8.7kg is made, through detecting egg
The gross mass that white matter accounts for microscapsule powder total weight is 28.8%, and it is total that polypeptide (molecular weight≤10000) accounts for microscapsule powder
The 21.2% of weight, surface oil 0.12%, powder color are milky.62 DEG C of destructive testings, peroxide value after the 45th day
2.6meq/kg, powder color slightly turn yellow than before, are faint yellow.
Embodiment 4
(1) wheat gluten for weighing 5kg adds the distilled water of 20kg to stir and is heated to 37 DEG C, and adjusting PH to 2 is added
The pepsin of 20000000 units hydrolyzes 10 minutes, mixed liquor is heated to 80 DEG C later, keeps the temperature enzyme deactivation in 20 minutes.Again by liquid
Temperature is down to 55 DEG C, and the bromelain of 40,000,000 units is added, and hydrolyzes 25 minutes, mixed liquor is heated to 80 DEG C later,
Keep the temperature enzyme deactivation in 20 minutes.
(2) sucrose fatty ester of the sucrose, 0.1kg that weigh 2.88kg is added in (1), is stirred evenly and in 80 DEG C, is protected
Temperature 30 minutes, this is water phase.
(3) vitamin E for weighing 0.02kg, which is added in the DHA algal oil of 2kg, to stir evenly, in the closed container for rushing nitrogen
In, 70 DEG C are heated to, keeps the temperature 45 minutes, this is oily phase.
(4) oil is mutually squeezed into water phase under stirring conditions, is sheared 3 minutes with cutter, synchronizes and be cooled to 60 DEG C, it
Afterwards homogeneous 2 times under the conditions of homogenization pressure is 30MP, it is finally spray-dried the DHA microscapsule powder that 8.2kg is made, through detecting egg
The gross mass that white matter accounts for microscapsule powder total weight is 8.8%, and polypeptide (molecular weight≤10000) accounts for microscapsule powder gross weight
The 41.2% of amount, surface oil 0.64%, powder color are milky.62 DEG C of destructive testings, peroxide value after the 45th day
4.2meq/kg, powder color slightly turn yellow than before, are faint yellow.
Embodiment 5
(1) pea protein for weighing 5kg adds the distilled water of 15kg to stir and is heated to 37 DEG C, and adjusting PH to 2 is added 1500
The pepsin of ten thousand units hydrolyzes 8 minutes, mixed liquor is heated to 80 DEG C later, keeps the temperature enzyme deactivation in 20 minutes.Again by fluid temperature
55 DEG C are down to, the bromelain of 30,000,000 units is added, hydrolyzes 25 minutes, mixed liquor is heated to 80 DEG C later, heat preservation 20
Minute enzyme deactivation.
(2) soybean lecithin of the trehalose, 0.1kg that weigh 1.88kg stirs evenly and in (1) in 75 DEG C, heat preservation 25
Minute, this is water phase.
(3) tea polyphenols for weighing 0.02kg, which are added in the DHA algal oil of 3kg, to stir evenly, in the closed container for rushing nitrogen,
70 DEG C are heated to, keeps the temperature 45 minutes, this is oily phase.
(4) oil is mutually squeezed into water phase under stirring conditions, is sheared 4 minutes with cutter, synchronizes and be cooled to 60 DEG C, it
Afterwards homogeneous 3 times under the conditions of homogenization pressure is 30MP, it is finally spray-dried the DHA microscapsule powder that 8.9kg is made, through detecting egg
The gross mass that white matter accounts for microscapsule powder total weight is 17.5%, and it is total that polypeptide (molecular weight≤10000) accounts for microscapsule powder
The 32.5% of weight, surface oil 0.25%, powder color are milky.62 DEG C of destructive testings, peroxide value after the 45th day
3.1meq/kg, powder color slightly turn yellow than before, are faint yellow.
Comparative example 1
The preparation method of this comparative example and embodiment 1 is essentially identical, the difference is that only: in step (1), weighing 5kg
Milk albumin adds the distilled water of 10kg to stir and is heated to 37 DEG C, dissolves lactalbumin sufficiently, and 20,000,000 units are added
Trypsase hydrolyzes 2 minutes, mixed liquor is heated to 80 DEG C later, keeps the temperature enzyme deactivation in 20 minutes；Fluid temperature is down to 60 again
DEG C, the papain of 25,000,000 units is added, hydrolyzes 30 minutes, mixed liquor is heated to 80 DEG C later, heat preservation is gone out for 20 minutes
Enzyme.
Thus obtained DHA microscapsule powder, which is detected protein and accounts for the gross mass of microscapsule powder total weight, is
39.4%, polypeptide (molecular weight≤10000) accounts for the 10.6% of microscapsule powder total weight, surface oil 0.2%, powder face
Color is milky.62 DEG C of destructive testings, peroxide value 2.8meq/kg after the 45th day, powder color change very greatly than before, are
Dark yellow analyzes reason, it may be possible to which polypeptide has color-protecting function under the high temperature conditions.
Comparative example 2
The preparation method of this comparative example and embodiment 1 is essentially identical, the difference is that only: in step (1), weighing 5kg
Milk albumin adds the distilled water of 10kg to stir and is heated to 37 DEG C, dissolves lactalbumin sufficiently, and 50,000,000 units are added
Trypsase hydrolyzes 15 minutes, mixed liquor is heated to 80 DEG C later, keeps the temperature enzyme deactivation in 20 minutes；Fluid temperature is down to 60 again
DEG C, the papain of 25,000,000 units is added, hydrolyzes 30 minutes, mixed liquor is heated to 80 DEG C later, heat preservation is gone out for 20 minutes
Enzyme.
The gross mass that thus obtained DHA microscapsule powder accounts for microscapsule powder total weight through detecting protein is 4.6%,
Polypeptide (molecular weight≤10000) accounts for the 45.4% of microscapsule powder total weight, and surface oil is 8.4% (general surface oil height
In 1%, that is, think no embedded), since surface oil is too high, do not do necessity of stability.
Microscapsule powder made from this comparative example embeds poor effect, very with microscope oil mirror microscopy discovery lipochondrion
Greatly.
Comparative example 3
The preparation method of this comparative example and embodiment 1 is essentially identical, the difference is that only: in step (1), weighing 5kg
Milk albumin adds the distilled water of 10kg to stir and is heated to 37 DEG C, dissolves lactalbumin sufficiently, and 20,000,000 units are added
Trypsase hydrolyzes 5 minutes, mixed liquor is heated to 80 DEG C later, keeps the temperature enzyme deactivation in 20 minutes；Fluid temperature is down to 60 again
DEG C, the bromelain of 25,000,000 units is added, hydrolyzes 30 minutes, mixed liquor is heated to 80 DEG C later, heat preservation is gone out for 20 minutes
Enzyme.
The gross mass that thus obtained DHA microscapsule powder accounts for microscapsule powder total weight through detecting protein is 2.8%,
Polypeptide (molecular weight≤10000) accounts for the 47.2% of microscapsule powder total weight, surface oil 18.2%, too due to surface oil
Height does not do necessity of stability.
There are many concrete application approach of the present invention, the above is only a preferred embodiment of the present invention.More than it should be pointed out that
Embodiment is merely to illustrate the present invention, and the protection scope being not intended to restrict the invention.For the common skill of the art
For art personnel, without departing from the principle of the present invention, several improvement can also be made, these improvement also should be regarded as this hair
Bright protection scope.
Claims (7)
1. a kind of DHA grease microscapsule powder containing polypeptide, which is characterized in that each group including following mass percentage
Point:
The polypeptide and protein are to be obtained by protein by the endless all-hydrolytic of protease；The protein is bovine whey
When albumen and/or sheep lactalbumin, hydrolysis is combined with trypsase and papain；The protein is soybean egg
When at least one of white, wheat gluten, pea protein, hydrolysis is combined with pepsin and bromelain.
2. the DHA grease microscapsule powder according to claim 1 containing polypeptide, which is characterized in that the carbon aquation
Conjunction object is the mixture of one or more of lactose, maltodextrin, maltose, sucrose, trehalose, glucose.
3. the DHA grease microscapsule powder according to claim 1 containing polypeptide, which is characterized in that the emulsifier
For the mixture of one or more of monoglyceride, soybean lecithin, modified phospholipid, sucrose fatty ester；The antioxidant is
The mixture of one or more of tea polyphenols, ascorbyl palmitate, sodium ascorbate, vitamin E.
4. a kind of preparation method of the DHA grease microscapsule powder according to claim 1 containing polypeptide, feature exist
In, comprising the following steps:
A, protein is not exclusively digested, prepares the mixture of protein and polypeptide；
B, carbohydrate, emulsifier are added in the mixture of protein made from step A and polypeptide, stirring is equal
It is even, and 20~30 minutes are kept the temperature at 70~80 DEG C, obtain water phase；
C, it adds antioxidants in DHA algal oil and stirs evenly, in the closed container of inflated with nitrogen, be added at 60~80 DEG C
Heat preservation 30~60 minutes, obtains oily phase；
D, under agitation, oil is mutually squeezed into water phase, is sheared 3~5 minutes with cutter, is synchronized and be cooled to 60 DEG C, then
Homogeneous 2~3 times under the conditions of pressure is 25~45MPa, then it is spray-dried up to the DHA grease microscapsule powder.
5. the preparation method of the DHA grease microscapsule powder according to claim 4 containing polypeptide, which is characterized in that
In step A, when the protein is milk albumin and/or sheep lactalbumin, it is specific the preparation method is as follows:
A1, protein is weighed, 2~4 times of distilled water is added to stir and is heated to 37 DEG C, dissolve albumen sufficiently；
Protease 1 is added in A2, the solution made from step A1, hydrolyzes 5~15 minutes, is then heated to 80 DEG C, keeps the temperature 20 points
Clock enzyme deactivation；
A3, by step A2, treated that solution is cooled to 55~60 DEG C, adds protease 2, hydrolyzes 25~30 minutes, then plus
Heat keeps the temperature enzyme deactivation in 20 minutes to 80 DEG C；Up to the mixture of the protein and polypeptide；
The protease 1 is trypsase or pepsin；Protease 2 is papain or bromelain.
6. the preparation method of the DHA grease microscapsule powder according to claim 5 containing polypeptide, which is characterized in that
The protein be milk albumin and/or sheep lactalbumin when, protease 1 be trypsase, enzyme be added concentration be 4000~
8000U/g；Protease 2 is papain, and it is 5000~10000U/g that concentration, which is added, in enzyme.
7. the preparation method of the DHA grease microscapsule powder according to claim 5 containing polypeptide, which is characterized in that
When the protein is at least one of soybean protein, wheat gluten, pea protein, protease 1 is pepsin, and enzyme is added
Concentration is 2000~4000U/g；Protease 2 is bromelain, and it is 4000~8000U/g that concentration, which is added, in enzyme.
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| CN108260807A (en) * | 2018-01-25 | 2018-07-10 | 郑州市云鼎汇砂生物科技有限公司 | It is a kind of that there is fermented food of the healthcare function containing small-molecular peptides |
| CN108753457A (en) * | 2018-08-03 | 2018-11-06 | 梁云 | The method for improving microbial grease stability and safety |
| CN109123660B (en) * | 2018-09-17 | 2021-07-23 | 山西欧莱特农业科技有限公司 | Walnut whey protein composite powder for pregnant woman nutriments and preparation method thereof |
| CN109198045A (en) * | 2018-10-09 | 2019-01-15 | 嘉必优生物技术（武汉）股份有限公司 | A kind of grease microcapsule and preparation method thereof |
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| CN110547457A (en) * | 2019-09-02 | 2019-12-10 | 嘉必优生物技术（武汉）股份有限公司 | Polyunsaturated fatty acid microcapsule and preparation method and application thereof |
| CN110692893A (en) * | 2019-11-12 | 2020-01-17 | 南通厚元生物科技有限公司 | Preparation method of solid beverage containing phosphatidylserine and omega-3 fatty acid |
| CN112450390B (en) * | 2020-10-30 | 2024-04-09 | 浙江波拉波拉食品股份有限公司 | Fish sausage containing DHA microcapsule and preparation method thereof |
| CN115553466B (en) * | 2022-09-30 | 2024-05-31 | 南京益唯森生物科技有限公司 | Nitraria seed oil microcapsule powder containing flax polypeptide and preparation method thereof |
| CN117568176B (en) * | 2023-10-08 | 2024-06-07 | 山东悦翔生物有限公司 | DHA extraction process based on microalgae extraction |
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| CN101260421A (en) * | 2008-04-28 | 2008-09-10 | 浙江省农业科学院 | Method for preparing whey protein peptide by composite protease hydrolysis |
| CN101884415B (en) * | 2010-06-18 | 2012-11-28 | 南京工业大学 | DHA microcapsules and preparation technology thereof |
| CN102771781A (en) * | 2011-05-07 | 2012-11-14 | 于文利 | Preparation method of novel nano DNA nutrition powder |
| CN103549442B (en) * | 2013-11-01 | 2015-09-09 | 厦门汇盛生物有限公司 | A kind of DHA algal oil microcapsule powder of high fuel load and preparation technology thereof |
| CN106615233A (en) * | 2016-09-13 | 2017-05-10 | 广东瑞博制药有限公司 | Preparation method for synchronously extracting spirulina grease and hydrolyzed proteins by aid of complex enzyme processes |
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