CN105154213A - Extraction method for tea seed oil containing conjugated linoleic acid - Google Patents
Extraction method for tea seed oil containing conjugated linoleic acid Download PDFInfo
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- CN105154213A CN105154213A CN201510438143.5A CN201510438143A CN105154213A CN 105154213 A CN105154213 A CN 105154213A CN 201510438143 A CN201510438143 A CN 201510438143A CN 105154213 A CN105154213 A CN 105154213A
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Abstract
The invention discloses an extraction method for tea seed oil containing conjugated linoleic acid. The method comprises the following steps: (1) preparing tea seed powder: shelling tea seeds, crushing the tea seeds, screening the crushed tea seeds, and drying the crushed tea seeds until the weight is constant to obtain tea seed powder; (2) carrying out extraction by virtue of an aqueous enzymatic method: weighing the tea seed powder according to a material-to-liquid weight/volume ratio of 1:2 to 1:10; adding a part of water into the tea seed powder, heating the water with the tea seed powder for 5-15 minutes to inactivate enzymes of the tea seed powder, cooling the water with the tea seed powder to room temperature, adding a prepared compound preparation of pectinase and linoleate isomerase and residual water to obtain a tea seed powder mixed liquid; uniformly stirring the liquid, carrying out enzymolysis at 30-60 DEG C in a stirring condition for 1.5-5 hours; and then sequentially carrying out secondary enzyme deactivation, centrifugation and separation to obtain an oil sample. According to the extraction method disclosed by the invention, linoleic acid is synchronously isomerized by combining an oil extraction process by virtue of the aqueous enzymatic method. Tea seed oil containing conjugated linoleic acid is developed for the first time in an oil extraction process so as to further improve the nutritional value of the tea seed oil, so that wider space is provided for technical development of the aqueous enzymatic method.
Description
Technical field
The present invention relates to the extracting method of tea seed oil, be specifically related to a kind of extracting method of the tea seed oil containing conjugated linolic acid.
Background technology
Tea seed oil is the grease that Theaceae Camellia Plants tea tree (CamelliaSinensisO.Ktze) fruit extracts, and is new resources oil product by the official approval of the Ministry of Health of China.Tea seed oil lipid acid composition is main based on unsaturated fatty acids, and its mid-oleic is 46.33% ~ 55.48%, linoleic acid content is 23.17% ~ 29.40%.Linoleic acid isomers conjugated linolic acid becomes study hotspot in recent years, its have antitumor, anti-oxidant, atherosclerosis, raising immunizing power, etc. multiple important physiological function.The distribution of occurring in nature conjugated linolic acid is very limited, to exist only in the greases such as some ruminating animal cattle and sheep and content is lower.
At present, the synthesis of conjugated linolic acid mainly contains chemical acid alkali catalytic isomerization method and biological enzyme isomerate process.The conjugated linoleic acid isomers of chemical method synthesis is various, and structure is not single, brings hidden danger to food and medicine safety; It is single that biological process can realize product isomer preferably, and to natural to there is CLA isomer similar, and the features such as safety non-toxic receive the concern of investigator.
Aqueous enzymatic method utilizes the plant cell components such as bio-enzyme degradation Mierocrystalline cellulose, pectin to impel grease to discharge, and treatment condition are gentle.Research finds that in grease aqueous enzymatic extraction system, glyceride exists with the form of emulsification, sterically hindered less between each lipid acid, easily carries out enzymatic modification to lipid acid.
For the present situation of above-mentioned prior art, contriver carries out deep research to the extraction process of tea seed oil, plan utilizes tea seed oil aqueous enzymatic extraction reaction system feature, the enzymatic reaction of direct grafting linoleic acid isomerization, the tea seed oil of exploitation containing conjugated linolic acid.
Summary of the invention
The object of this invention is to provide a kind of extracting method of the tea seed oil containing conjugated linolic acid, it can prepare the tea seed oil that end product contains conjugated linolic acid.
To achieve these goals, the present invention adopts following technical scheme:
Containing the extracting method of the tea seed oil of conjugated linolic acid, comprise the following steps:
(1) tea-seed powder preparation: tea-seed shells, and pulverizes and sieves, and dries to constant weight, obtains tea-seed powder;
(2) aqueous enzymatic extraction: be 1:2-1:10 according to feed liquid weight/volume, take tea-seed powder, part water is first added in this tea-seed powder, heat 5-15 minute to eliminate self enzymic activity of tea-seed powder, then room temperature is cooled to, add the compound formulation of polygalacturonase and the linoleate isomerase configured and remaining water, obtain tea-seed powder mixed solution, stir, at 30-60 DEG C, enzymolysis 1.5-5h under agitation condition, then carry out successively secondary go out enzyme, centrifugal, be separated after obtain oil sample;
In the compound formulation of described polygalacturonase and linoleate isomerase, the mass percent concentration of polygalacturonase is 0.1%-1.0%, and consumption is 1.0-20mL/g tea-seed powder; The consumption of linoleate isomerase is 1.0-20mL/g tea-seed powder.
After adopting such scheme, the inventive method adopts isomerase carry out synchronous and heterogeneousization to linolic acid in extracting tea seed oil by using enzymatic hydrolysis process, tea seed oil is extracted and conjugated linolic acid integrator, obtain the tea seed oil containing conjugated linolic acid, in enzymolysis solution, CLA concentration can reach 43.50 μ g/mL, and with this understanding, oil extracting rate reaches 26.2%, analyze after testing, the tea seed oil cla levels 0.27% of exploitation.Tea seed oil fatty acid species can be enriched by the inventive method, improve its health value, for functional grease exploitation provides new thought.
Embodiment
Embodiment one:
The present invention, containing the extracting method of the tea seed oil of conjugated linolic acid, comprises the following steps:
(1) tea-seed powder preparation: tea-seed shells, and pulverizes and sieves, and dries to constant weight, obtains tea-seed powder;
(2) aqueous enzymatic extraction: be 1:5 according to feed liquid weight/volume, take tea-seed powder 10g, part water is first added in this tea-seed powder, heat 10 minutes to eliminate self enzymic activity of tea-seed powder, then room temperature is cooled to, add the compound formulation of polygalacturonase and the linoleate isomerase configured and remaining water, obtain tea-seed powder mixed solution, stir, at 43 DEG C, enzymolysis 3.5h under agitation condition, obtain enzymolysis solution, then to enzymolysis solution carry out successively secondary go out enzyme, centrifugal, be separated after obtain oil sample;
In the compound formulation of described polygalacturonase and linoleate isomerase, the mass percent concentration of polygalacturonase is 0.2%, and consumption is 6.86mL/g tea-seed powder; The consumption of linoleate isomerase is 5.58mL/g tea-seed powder.
The present embodiment is most preferred embodiment of the present invention, and in the present embodiment, in enzymolysis solution, CLA concentration can reach 43.50 μ g/mL, and with this understanding, oil extracting rate reaches 26.2%.
Embodiment two:
The present invention, containing the extracting method of the tea seed oil of conjugated linolic acid, comprises the following steps:
(1) tea-seed powder preparation: tea-seed shells, and pulverizes and sieves, and dries to constant weight, obtains tea-seed powder;
(2) aqueous enzymatic extraction: be 1:2 according to feed liquid weight/volume, take tea-seed powder 10g, part water is first added in this tea-seed powder, heat 5 minutes to eliminate self enzymic activity of tea-seed powder, then room temperature is cooled to, add the compound formulation of polygalacturonase and the linoleate isomerase configured and remaining water, obtain tea-seed powder mixed solution, stir, at 30 DEG C, enzymolysis 1.5h under agitation condition, obtain enzymolysis solution, then to enzymolysis solution carry out successively secondary go out enzyme, centrifugal, be separated after obtain oil sample;
In the compound formulation of described polygalacturonase and linoleate isomerase, the mass percent concentration of polygalacturonase is 0.5%, and consumption is 3mL/g tea-seed powder; The consumption of linoleate isomerase is 13mL/g tea-seed powder.
In the present embodiment, in enzymolysis solution, CLA concentration reaches 35.9 μ g/mL, and with this understanding, oil extracting rate reaches 25.1%.
Embodiment three:
The present invention, containing the extracting method of the tea seed oil of conjugated linolic acid, comprises the following steps:
(1) tea-seed powder preparation: tea-seed shells, and pulverizes and sieves, and dries to constant weight, obtains tea-seed powder;
(2) aqueous enzymatic extraction: be 1:2 according to feed liquid weight/volume, take tea-seed powder 10g, part water is first added in this tea-seed powder, heat 5 minutes to eliminate self enzymic activity of tea-seed powder, then room temperature is cooled to, add the compound formulation of polygalacturonase and the linoleate isomerase configured and remaining water, obtain tea-seed powder mixed solution, stir, at 30 DEG C, enzymolysis 1.5h under agitation condition, obtain enzymolysis solution, then to enzymolysis solution carry out successively secondary go out enzyme, centrifugal, be separated after obtain oil sample;
In the compound formulation of described polygalacturonase and linoleate isomerase, the mass percent concentration of polygalacturonase is 0.5%, and consumption is 13mL/g tea-seed powder; The consumption of linoleate isomerase is 7mL/g tea-seed powder.
In the present embodiment, in enzymolysis solution, CLA concentration reaches 34.8 μ g/mL, and with this understanding, oil extracting rate reaches 24.5%.
Embodiment four:
The present invention, containing the extracting method of the tea seed oil of conjugated linolic acid, comprises the following steps:
(1) tea-seed powder preparation: tea-seed shells, and pulverizes and sieves, and dries to constant weight, obtains tea-seed powder;
(2) aqueous enzymatic extraction: be 1:10 according to feed liquid weight/volume, take tea-seed powder 10g, part water is first added in this tea-seed powder, heat 15 minutes to eliminate self enzymic activity of tea-seed powder, then room temperature is cooled to, add the compound formulation of polygalacturonase and the linoleate isomerase configured and remaining water, obtain tea-seed powder mixed solution, stir, at 60 DEG C, enzymolysis 5h under agitation condition, obtain enzymolysis solution, then to enzymolysis solution carry out successively secondary go out enzyme, centrifugal, be separated after obtain oil sample;
In the compound formulation of described polygalacturonase and linoleate isomerase, the mass percent concentration of polygalacturonase is 1.0%, and consumption is 10mL/g tea-seed powder; The consumption of linoleate isomerase is 5mL/g tea-seed powder.
In the present embodiment, in enzymolysis solution, CLA concentration can reach 25.3 μ g/mL, and with this understanding, oil extracting rate reaches 23.8%.
Embodiment five:
The present invention, containing the extracting method of the tea seed oil of conjugated linolic acid, comprises the following steps:
(1) tea-seed powder preparation: tea-seed shells, and pulverizes and sieves, and dries to constant weight, obtains tea-seed powder;
(2) aqueous enzymatic extraction: be 1:10 according to feed liquid weight/volume, take tea-seed powder 10g, part water is first added in this tea-seed powder, heat 15 minutes to eliminate self enzymic activity of tea-seed powder, then room temperature is cooled to, add the compound formulation of polygalacturonase and the linoleate isomerase configured and remaining water, obtain tea-seed powder mixed solution, stir, at 60 DEG C, enzymolysis 5h under agitation condition, obtain enzymolysis solution, then to enzymolysis solution carry out successively secondary go out enzyme, centrifugal, be separated after obtain oil sample;
In the compound formulation of described polygalacturonase and linoleate isomerase, the mass percent concentration of polygalacturonase is 1.0%, and consumption is 1mL/g tea-seed powder; The consumption of linoleate isomerase is 13mL/g tea-seed powder.
In the present embodiment, in enzymolysis solution, CLA concentration can reach 20.5 μ g/mL, and with this understanding, oil extracting rate reaches 23%.
Claims (1)
1., containing the extracting method of the tea seed oil of conjugated linolic acid, it is characterized in that: comprise the following steps:
(1) tea-seed powder preparation: tea-seed shells, and pulverizes and sieves, and dries to constant weight, obtains tea-seed powder;
(2) aqueous enzymatic extraction: be 1:2-1:10 according to feed liquid weight/volume, take tea-seed powder, part water is first added in this tea-seed powder, heat 5-15 minute to eliminate self enzymic activity of tea-seed powder, then room temperature is cooled to, add the compound formulation of polygalacturonase and the linoleate isomerase configured and remaining water, obtain tea-seed powder mixed solution, stir, at 30-60 DEG C, enzymolysis 1.5-5h under agitation condition, then carry out successively secondary go out enzyme, centrifugal, be separated after obtain oil sample;
In the compound formulation of described polygalacturonase and linoleate isomerase, the mass percent concentration of polygalacturonase is 0.1%-1.0%, and consumption is 1.0-20mL/g tea-seed powder; The consumption of linoleate isomerase is 1.0-20mL/g tea-seed powder.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106929147A (en) * | 2017-03-10 | 2017-07-07 | 河南工业大学 | A kind of method for preparing oil body |
Citations (3)
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| CN1597891A (en) * | 2004-08-20 | 2005-03-23 | 彭汝祥 | Process of extracting glasswort oil from glasswort plant |
| WO2007122730A1 (en) * | 2006-04-18 | 2007-11-01 | Itochu Corporation | Processed food and production method and use thereof |
| CN102181319A (en) * | 2011-04-06 | 2011-09-14 | 贵州大学 | Oil making method based on lower-moisture enzymatic auxiliary cold pressing |
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2015
- 2015-07-23 CN CN201510438143.5A patent/CN105154213A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1597891A (en) * | 2004-08-20 | 2005-03-23 | 彭汝祥 | Process of extracting glasswort oil from glasswort plant |
| WO2007122730A1 (en) * | 2006-04-18 | 2007-11-01 | Itochu Corporation | Processed food and production method and use thereof |
| CN102181319A (en) * | 2011-04-06 | 2011-09-14 | 贵州大学 | Oil making method based on lower-moisture enzymatic auxiliary cold pressing |
Non-Patent Citations (1)
| Title |
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| 王晓琴: ""响应面分析水酶法提取茶叶籽油工艺优化研究"", 《中国粮油学报》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106929147A (en) * | 2017-03-10 | 2017-07-07 | 河南工业大学 | A kind of method for preparing oil body |
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