CN106929147A - A kind of method for preparing oil body - Google Patents
A kind of method for preparing oil body Download PDFInfo
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- CN106929147A CN106929147A CN201710140163.3A CN201710140163A CN106929147A CN 106929147 A CN106929147 A CN 106929147A CN 201710140163 A CN201710140163 A CN 201710140163A CN 106929147 A CN106929147 A CN 106929147A
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- enzyme
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B1/00—Production of fats or fatty oils from raw materials
- C11B1/10—Production of fats or fatty oils from raw materials by extracting
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23D—EDIBLE OILS OR FATS, e.g. MARGARINES, SHORTENINGS, COOKING OILS
- A23D9/00—Other edible oils or fats, e.g. shortenings, cooking oils
- A23D9/02—Other edible oils or fats, e.g. shortenings, cooking oils characterised by the production or working-up
- A23D9/04—Working-up
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- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Edible Oils And Fats (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The invention provides a kind of method for preparing oil body, wherein methods described includes：I () provides oil plant；(ii) by the oil plant in the presence of water and enzyme, cellulose, pectin, hemicellulose or glucan in destruction cell membrane and/or destruction cell membrane；(iii) isolated oil body.
Description
Technical field
The invention belongs to a kind of food, health food or biological technical field, specifically, being related to a kind of safe and pollution-free
The method for preparing oil plant oil body.
Background technology
Oil crops can provide abundant vegetable protein and oil body, large agricultural product necessary to be both daily life, be also
Important raw materials for food industry.The plantation of China oil crops, processing and its resulting economic benefit are only second to grain
Crop, the effective supply and utilization of oil product are the important components for ensureing national food safety.The plantation of China's oil plant
Mainly including rape, peanut, soybean, sesame, flax and sunflower etc., total output accounts for the 90% of oil crops.At present, China
Oil processing enterprise more show booming, but most enterprise uses the product nutrition of traditional approach, processing to keep level
Relatively low, value-added content of product is not high.Deep processing of the enterprise to oil plant is limited in one's ability, and scientific and technological content is relatively low, restricts to a certain extent
The sound development of oil plant industry.
The exploitation of oil plant oil body are the effective ways for improving oil resource utilization ratio.At present, it is most of both at home and abroad
The oil body of oil plant is extracted and uses chemical method and aqua method.There is residual chemical agents, nutriment to some extent in these methods
The shortcomings of loss is serious, extraction efficiency is not high.Exploitation is suitable for the safe and efficient method of industrial separation oil plant oil body, contributes to
The sound development of oil plant industry.
Therefore, anxious at present scarce a kind of green safety, the simple method of operating procedure efficiently prepare the work of oil body from oil plant
Skill.
The content of the invention
The first object of the present invention is to obtain a kind of method for preparing oil body, and the method is to current aqua method extract oil
Expect the improvement of oil body technique, overcome the deficiency that extraction time is long, oil body recovery rate is low in aqua method technique, obtain a kind of fat
Fat content oil body high.
Methods described disclosed by the invention includes：
I () provides the oil plant crushed；(ii) by the oil plant in the presence of water and enzyme, cell membrane and/or destruction are destroyed
Cellulose, pectin, hemicellulose or glucan in cell membrane；(iii) oil body for obtaining will be reacted to separate；Preferably, it is described
Oil body fat content be more than 60%, more preferably more than 80%, most preferably more than 90%.
Method as described above, wherein described enzyme is selected from cellulase, pectase, hemicellulase, dextranase
Any one or a few any combination.
Method as described above, wherein enzyme, hydrolytic phosphatide class knot of the described enzyme not comprising aminosal and/or peptides
The phosphatidase of structure.
Method as described above, wherein described oil plant includes protein and oil body.
Method as described above, wherein carrying out pulverization process before the step (i).
Method as described above, described oil plant is selected from the seed or core obtained in plant, preferably palm fruit, palm kernel,
Peanut, oat, corn, rapeseed, soybean, sesame, linseed, sunflower, cottonseed, kardiseed, purple perilla seed, tea seed, castor bean,
Coconut, olive, cocoa bean, Chinese tallow seed, almond benevolence, almond, bancoul nuts, rubber seed, rice bran, maize, wheat, sesame
Seed, castor bean, linseed, oenothera seed, fibert, pumpkin, English walnut, grape pip, flaxseed, borage seed, seabuckthorn seeds, tomato
Seed, pumpkin, Queensland nut, cocoa, algae and/or their any mixture.
Method as described above, wherein described oil plant is 1 with the part by weight of water:3-10, preferably 1:3-8.
Method as described above, wherein described method includes being dried the oil body that step (iii) is obtained.
Method as described above, wherein described dries using vacuum drying.
A kind of oil body prepared using method as described above, the fat mass content wherein in oil body is 60-
95%, protein quality content is 3-30%, and ash content is not higher than 0.5%, also including total reducing sugar and/starch 0.5-10%
Food, medicine, infant are being prepared the invention also discloses the oil body that a kind of method as described above is prepared
Application in care product.
Oil crops refer to the herbaceous plant such as soybean, peanut, rape, the sesame planted extensively in China.In oil crops
Grease and protein exist in the form of oil body and proteosome respectively, and oil body is that (predominantly trigalloyl is sweet by substantial amounts of neutral fat
Oil) and a small amount of phosphatide and protein composition, the metastable subcellular fraction droplet in oil plant cell is dispersed in, these droplets are again
Referred to as fat drips, spherosome (spherosomes), grease body (oleosomes) or oil body (oil bodies).
When oil plant is crushed, the average grain diameter of crushing is 5-200 μm, most preferably preferably 10-150 μm, 10-80 μm.
The increase for crushing number of times is together decided on the nutriment of grease, grinding time is lost by oil plant species and pulverizer type, is protected
Card powder particle diameter general control is particle diameter close to oil plant cell, preferably less than oil plant cell particle diameter and be more than oil body particle diameter.
In the specific embodiment of the present invention, with cell wall damage effect enzyme mainly include cellulase,
One or more of pectase, hemicellulase etc., preferably comprise one or two in cellulase or pectase；In this hair
In a bright specific embodiment, described enzyme is selected from and gathers containing cellulose, pectin, hemicellulose and Portugal in destruction cell membrane
The enzyme of sugared structure, such as from Novi's letter and the enzyme of sigma companies.In preferred embodiments of the disclosure scheme,
Extraction process preferably does not contain the enzyme of following species：Enzyme and hydrolytic phosphatide class formation with aminosal and peptides
Phosphatide enzyme.
In the specific embodiment of the present invention, described enzyme is preferably pectase, one kind of cellulase or two
The mixture of person, or the commercial complex cellulase acted on hydrolysis of pectin, cellulose.It is specific real at one of the invention
Apply in mode, at 3000-5000U/g (mL), addition should be according to examination according to the species difference of oil crops for the enzyme activity of the enzyme
Determination is tested, is added in the form of solid or liquid；Reaction condition is typically to determine Extracting temperature according to the optimum temperature of enzyme,
Extraction time is so that the recovery rate of grease body can routinely adjustment reaches highest and perseverance with the general knowledge of those skilled in the art
It is fixed.
Need to be slightly agitated for or homogeneous after enzyme preparation addition, rotating speed and the time of control stirring or homogeneous, prevent from forming tight
The emulsion of weight.
Separation means as described above include various conventional means, and its non-limitative example includes taking centrifugation, filtering etc..
Described centrifugation can so make the non-aqueous in oilseed protein using centrifuge, 4000~8000 revs/min of rotation speed requirements
Property protein is sufficiently separated precipitation.
Oil body is the milky milk oil body of semi-solid, and the different yield according to oil plant species are had any different, the composition of oil body
Contain grease, protein, phosphatide, carbohydrate etc..Emulsifying agent based on oil body can be used for food, health products, medicine, personal shield
Reason product etc..
This law destroys oil plant eucaryotic cell structure by using biological enzyme formulation, promotes the release of oil body, and this method can not only be obtained
The oil plant oil body of safe nutrition is obtained, while also pollution will not be produced to environment, it is adaptable to the large-scale industry metaplasia of oil plant oil body
Produce.
Brief description of the drawings
Fig. 1, Fig. 2 are the zeta current potentials of peanut oil body with salinity, the change of pH.
Specific embodiment
The present inventor, by improving preparation technology, oil body skill is extracted for existing at present by in-depth study extensively
In art, it is readily incorporated adventitious solvent and makes food by chemical reagent pollution pollution, loses the shortcoming of natural nutrition value, and mesh
It is preceding to prepare the relatively low problem of yield in oil body technique, it is contemplated that using zymotechnic so that production or preparation process are completely green
Color is produced, and prepares a kind of oil body of safe nutrition.The present invention is completed on this basis.
Technology design of the invention is as follows：
Instant invention overcomes the defect of prior art, it is proposed that a kind of method for preparing oil body, the technique combination enzyme process work
The advantage of skill, is improved on the basis of physics defibrination, and the method in existing process using water or chemical immersion is substituted
It is the method for enzymolysis, the time has been saved to a greater extent, energy consumption or waste has been reduced, is avoided the solvent being chemically incorporated into,
The yield higher than aqua method is made it have, or even its oil body for obtaining has wider purposes.Further, reduce
Other various adventitious solvents or the food-safe threat of chemicals are introduced, product reaches the standard of food security.
In the present invention, term " containing " or " including " represent various composition can together be applied to mixture of the invention or group
In compound.Therefore, term " mainly by ... constitute " and " consist of " be included in term " containing " or " including " in.
Hereinafter it is described in detail to various aspects of the present invention：
Raw material
In the present invention, the raw material for using is oil plant, and its seed or core for deriving from plant, its non-limitative example is included
Peanut, corn, rapeseed, soybean, sesame, linseed, sunflower, almond benevolence, almond, rice bran, maize, wheat, and/or
Their any mixture.
Oil body is to surround matrix triacylglycerol, the spherula that protein mosaic is formed on film, egg by one layer of immobilized artificial membrane
The structure of white matter and phosphatide maintains oil body stability and effectively protection grease unrighted acid in itself.Based on oil body
Emulsion can be used for food, health products, medicine, personal care product etc..Applicable product has mayonnaise, ice river in Henan Province in food
Pouring, pudding, fruit juice, sugar-coat etc.；In health products and medicine, the emulsion thing based on oil body can be used as the auxiliary system in vaccine
Agent and oral class medicine product composition；In personal care product, the emulsion based on oil body can be applied to face cream, emulsion, shield
Hair product, bath product etc..At present, by protein, oil and the artificial synthesized oil body emulsion of water homogenisation, but this emulsion is making
During can lose protein and the natural nutritive value of grease.
Enzyme
In the specific embodiment of the present invention, the enzyme with cell wall damage effect.
In the specific embodiment of the present invention, its non-limitative example of described enzyme includes cellulase, fruit
Any one or a few combination in glue enzyme, hemicellulase, dextranase.
In the specific embodiment of the present invention, described enzyme is to the cellulose in cell membrane, pectin, hemicellulose
And/or glucan structure is digested or destroyed.
One of the invention preferred embodiment in, the enzyme for being used does not contain aminosal and/or peptides
The phosphatide enzyme of enzyme or hydrolytic phosphatide class formation.
One of the invention preferred embodiment in, described enzyme is selected from the mixed of pectase, cellulase or both
Compound.
One of the invention preferred embodiment in, described enzyme is selected from complex cellulase, and described enzyme can be with
Hydrolysis of pectin and cellulose.
In the specific embodiment of the present invention, the enzyme activity of described enzyme is 3000-5000U/g (mL).The enzyme
Addition conventional adjustment is carried out according to the species difference of oil crops, in the specific embodiment of the present invention, institute
The enzyme dosage stated is 20-100U/g, and described enzyme dosage is that the dry weight of oil plant is radix.
In the specific embodiment of the present invention, described enzyme is added in the form of solid or liquid.
In the specific embodiment of the present invention, described enzyme is the form presence of immobilization or on-fixed.
In the specific embodiment of the present invention, described enzyme can be with appendix on various carriers, such as resin, dioxy
On SiClx, activated carbon, nano-substance and/or other analogs.
The method for preparing oil body
Preparation method of the present invention is using enzyme to the cellulose in the cell membrane or cell membrane in oil plant, pectin, half
Cellulose and glucan are acted on.
In the specific embodiment of the present invention, described oil plant is first crushed.
The described method for preparing oil body is comprised the following steps：
I () provides oil plant；
(ii) oil plant is reacted in the presence of water and enzyme as described above；
(iii) oil body for obtaining will be reacted to be separated.
Oil plant as described above before reaction, need to carry out homogeneous in the presence of water and enzyme；, it is necessary to stir or shake in reaction
Swing.In homogeneous, should prevent from forming serious emulsion.Described stirring or the speed of concussion can be according to skills in the art
The routine operation of art personnel is adjusted.
The reaction that described oil plant is carried out in the presence of water and enzyme, can use various enzyme reaction equipment, including but not
It is limited to fixed bed, retort, oscillator etc..
Described oil plant and the ratio of water can be adjusted according to the operation general knowledge of those skilled in that art.So that oil plant
Being capable of sufficient haptoreaction with enzyme.
In the specific embodiment of the present invention, described oil plant and the ratio of water are 1:3-10, preferably 1:3-
8, most preferably such as 1:3-7、1:3-6 or 1:4-5.
Preparation method as described above, after reaction terminates in step (ii), also including the process of inactivation.
Preparation method as described above, the step of separated in step (iii) in, the component of the eleoplast for obtaining is entered
It is vacuum dried at a temperature of row drying, non-limitative example such as 45-50 DEG C.
Oil body
Using preparation method as described above, the oil body for preparing (butt), its fat mass content is 60-95%；
Protein quality content is 3-30%, and ash content is not higher than 0.5%, also including total reducing sugar and/or starch 0.5-10%.
In the specific embodiment of the present invention, the fatty mass content of oil body is 60-90% (butt)；Egg
White matter mass content is 8-30%, and ash content is not higher than 0.4% (butt), also including total reducing sugar and/or starch 1-8%.
One of the invention preferred embodiment in, the oil body contains fat mass content for 65-90% is (dry
Base)；Protein content is 8-20%, and ash content is not higher than 0.3% (butt), also including total reducing sugar and/or starch 1-8%.
Using
Oil body in the present invention, can apply to multiple fields, such as food, medicine, infant nursing product, animal foodstuff
In application.
In the specific embodiment of the present invention, described oil body can be used for preparing ice cream.
Advantage
Present invention obtains oil body, with advantages below：
1st, it is medium extraction with enzyme and water, method is nontoxic, easy to operate, oil plant oil can be stablized and that purity is higher
Body, beneficial to the application in food；
2nd, the oil body that the present invention is extracted is rich in unrighted acid；
3rd, the oil body that the oil body that the present invention is extracted obtains being easy to preserving by vacuum drying, can rehydration obtain stablizing emulsus
Liquid, it is easy to operate, using extensive；
4th, the yield of resulting oil body is higher, can reach 40%, 45%, even up to more than 48%.
As no specific instructions, various raw materials of the invention can be by being commercially available；Or according to the routine side of this area
Method is prepared.Unless otherwise defined or described herein, all specialties used herein are ripe with art technology with scientific words
Practice meaning familiar to personnel institute identical.In addition any method similar to described content or impartial and material all can be applied to this
In inventive method.
Other aspects of the present invention, due to this disclosure, are to those skilled in the art apparent
's.
With reference to specific embodiment, the present invention is expanded on further.It should be understood that these embodiments are merely to illustrate the present invention
Rather than limitation the scope of the present invention.The experimental technique of unreceipted actual conditions in the following example, generally marks according to country
Standard is determined.If without corresponding national standard, being built according to general international standard, normal condition or according to manufacturer
The condition of view is carried out.Unless otherwise indicated, otherwise all of number is weight portion, and all of percentage is mass percent.
Unless otherwise defined or described herein, all specialties used herein and scientific words and the skilled people of art technology
Meaning familiar to member institute is identical.In addition any method similar to described content or impartial and material all can be applied to the present invention
In method.
Prepare embodiment：
With peanut seed as raw material, its processing method or step are as follows：
(1) crush：It is 30-100 μm to go skin peanuts pulverizer to be crushed to average grain diameter 20.00 ± 1.00g；
(2) homogeneous：After crushing, by peanut and deionized water in mass ratio 1:4 mixing, the composite plant of 66U/g peanuts
Hydrolase enzyme preparation, 18000r/min homogeneous 2s；
(3) digest：90min is shaken in 50 DEG C of water-bath constant temperature oscillators, fully enzymolysis；
(4) separating step is as follows：
A. enzymolysis is completed after the enzyme 5min that gone out in boiling water bath, cold water cooling after taking-up.The sample liquid of cooling is transferred to 400ml
Tool plug Centrifuge Cup is centrifuged 20min with 4000r/min, collects upper strata suspension；
B. sediment is cleaned again with remaining supernatant, be placed in 400ml Centrifuge Cups and 20min is centrifuged with 4000r/min,
Collect upper strata suspension；
C. collect supernatant and be placed in 400ml Centrifuge Cups with 4000r/min centrifugation 20min, collect upper strata suspension；
D. the suspension on the centrifuge tube upper strata of all collections is merged, that is, obtains oil body, oil body yield about 48-49%；Step
In the peanut oil body obtained in rapid d containing substantial amounts of higher unsaturated fatty acid (being shown in Table 2), especially oleic acid, linoleic contain
Amount is relatively enriched.Upper strata sediment is taken out, is placed in vacuum drying chamber, dry 10 hours remove moisture removal at a temperature of 45-50 DEG C.
Unrighted acid is aoxidized during oil body can be avoided, and makes its nutritive value；Its corresponding composition is shown in Table 1, other
Composition is total reducing sugar or starch etc.：
The peanut of table 1 and peanut oil body composition
The peanut oil body fat of table 2 acid mass percent composition
Fig. 1 shows the zeta current potentials of peanut oil body with the change of salinity, and its test method is：
Take 1g oil bodies respectively with 9g add different sodium chloride (0,10,40,80,100mmol/L) pH value (3.0,7.4,
9.0) 10mmol/L PBSs dilution, is made suspension, 20min is kept at 37 DEG C, then at room temperature (25 DEG C)
Lower placement 24h, is for about the solution of mass fraction 5% by suspension dilution, and dilution zeta electricity is determined with zeta potentiometric analyzers
Position.
Fig. 2 be peanut grease body isoelectric point (i.e. zeta current potentials with pH change) prediction, its experimental technique is：
Test method：1g oil bodies are taken to be delayed with the 10mmol/L phosphate of 9g pH value (3.0,4.0,5.0,6.0,7.0) respectively
Solution dilution is rushed, suspension is made, 20min is kept at 37 DEG C, then place 24h under room temperature (25 DEG C), suspension is diluted
For about the solution of mass fraction 5%, dilution zeta current potentials are determined with zeta potentiometric analyzers.
Application Example：
1. application of the oil body in ice cream
Ice cream is a kind of many as primary raw material with drinking water, cow's milk, milk powder, cream (or vegetable fat), sugar etc.
Phase dispersion.In the presence of emulsifying agent, not miscible in ice-cream slurry, the easy grease and water for forming layering produces mutual
Attract, the lipophilic group of oil soluble material and emulsifying agent is combined, the hydrophilic radical of water-soluble and emulsifying agent interacts, so as to produce
The raw interfacial film based on emulsifying agent.Emulsifying agent shields to the oil droplet in processing, make oil droplet be difficult coalescence, it is fully dispersed
Yu Shuixiang, so as to form relatively stable emulsion.The ice cream of such slurry production has the soft spy of delicate mouthfeel, tissue
Point.This research is by naturally occurring emulsifying agent --- peanut oil body is applied in the production of ice cream, it is ensured that cold drink food has uniqueness
Its alimentary health-care function can also be increased while local flavor, the addition of food industry emulsifying agent is reduced, this is to consumers in general
All it is significant with food and health products industry development.Grease body is a kind of good emulsifying agent, can make the emulsification of O/W types
Agent, after being added to ice cream, can be such that water, cow's milk, milk powder and sugar preferably mixes, and produce the local flavor and nutriture value of uniqueness
Value.
Application experiment
(1) it is formulated using the ice cream of oil body as follows：Water 60%, skimmed milk powder 12%, dilute cream 6%, sucrose 12%,
(oil body (the oil body prepared by above-described embodiment of oil body emulsion 10%:Water=8:2) it is well mixed).
(2) it is formulated using the ice cream of emulsifying agent as follows：Water 60%, milk powder 15%, dilute cream 12%, sucrose 12%, breast
Change stabilizer 0.6%.
Both color and lusters are slightly yellow, and the ice cream for adding vegetable oils has natural, light plant flavour, and adds
The ice cream delicate mouthfeel of oiling body is soft and smooth, without ice crystal, but using emulsifying agent ice cream then without corresponding feature.
Presently preferred embodiments of the present invention is the foregoing is only, substantial technological content model of the invention is not limited to
Enclose, substantial technological content of the invention is broadly to be defined in the right of application, any technology that other people complete
Entity or method, if identical with defined in the right of application, also or a kind of equivalent change, will
It is considered as being covered by among the right.
The all documents referred in the present invention are all incorporated as reference in this application, independent just as each document
It is incorporated as with reference to such.In addition, it is to be understood that after the above of the invention has been read, those skilled in the art can be right
The present invention makes various changes or modifications, and these equivalent form of values equally fall within the application appended claims limited range.
Claims (10)
1. it is a kind of to prepare the method for oil body, wherein the described method comprises the following steps：
I () provides the crushing oil plant of eleoplast and protein；
(ii) by the crushing oil plant in the presence of water and enzyme, cellulose, fruit in destruction cell membrane and/or destruction cell membrane
Glue, hemicellulose and/or glucan；
(iii) oil body for obtaining will be reacted to be separated.
2. the method for claim 1, wherein described enzyme is selected from cellulase, pectase, hemicellulase, glucan
Any one or a few any combination in enzyme.
3. the method for claim 1, wherein described enzyme do not contain aminosal and/or peptides and/or fat and/
Or the enzyme of phospholipid activity.
4. method as claimed in claim 2, wherein the activity of described enzyme is 100-5000U/g (mL), enzyme dosage 20-
100U/g。
5. the method as described in any one of claim 1-3 claim, described oil plant is selected from the seed obtained on plant
Or core, preferred palm fruit, palm kernel, peanut, oat, corn, rapeseed, soybean, sesame, linseed, sunflower, cottonseed, red
Flower seed, purple perilla seed, tea seed, castor bean, coconut, olive, cocoa bean, Chinese tallow seed, almond benevolence, almond, bancoul nuts, rubber seed,
Rice bran, maize, wheat, til seed, castor bean, linseed, oenothera seed, fibert, pumpkin, English walnut, grape pip, flax
Seed, borage seed, seabuckthorn seeds, Tomato Seed, pumpkin, Queensland nut, cocoa, algae and/or their any mixture.
6. method as claimed in claim 4, described oil plant is peanut.
7. the method as described in any one of claim 1-5 claim, wherein described oil plant is with the part by weight of water
1:3-10, preferably 1:3-8.
8. a kind of method as described in any one of claim 1-5 claim, wherein the fat in the oil body for preparing
Mass content is 60-95%, and protein quality content is 3-30%, and ash content is not higher than 0.5%, also including total reducing sugar and/or starch
0.5-10%.
9. a kind of oil body, its method for passing through described in any one of claim 1-5 claim is prepared, wherein fat
Content is 60-95%.
10. a kind of as the oil body that any one of claim 1-5 claim methods described is obtained is preparing food, medicine, baby
Application in baby care product, animal foodstuff.
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| CN108276482A (en) * | 2018-05-02 | 2018-07-13 | 云南神宇新能源有限公司 | A method of extracting oil body protein matter from jatropha curcas seed |
| CN108690705A (en) * | 2018-05-31 | 2018-10-23 | 镇江虎瑞生物科技有限公司 | A method of carrying out vegetable seed oil extract using microwave |
| WO2020187544A1 (en) * | 2019-03-19 | 2020-09-24 | Unilever Plc | Frozen confection |
| CN113854399A (en) * | 2021-11-04 | 2021-12-31 | 河南工业大学 | Method for extracting peanut oil body and protein by salt ion assisted aqueous enzymatic method |
| CN114128768A (en) * | 2021-11-26 | 2022-03-04 | 武汉轻工大学 | Rice bran oil body-curcumin mixed carrier and preparation method thereof |
| CN114981393A (en) * | 2020-01-15 | 2022-08-30 | 嘉吉公司 | Isolated oleosome compositions and methods of making the same |
| CN115990442A (en) * | 2023-02-10 | 2023-04-21 | 华南农业大学 | Tea oil microcapsule with double-layer core-shell structure and preparation method and application thereof |
| US12133539B2 (en) | 2019-03-19 | 2024-11-05 | Conopco, Inc. | Frozen confection |
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| WO2020187544A1 (en) * | 2019-03-19 | 2020-09-24 | Unilever Plc | Frozen confection |
| US12133539B2 (en) | 2019-03-19 | 2024-11-05 | Conopco, Inc. | Frozen confection |
| CN114981393A (en) * | 2020-01-15 | 2022-08-30 | 嘉吉公司 | Isolated oleosome compositions and methods of making the same |
| CN113854399A (en) * | 2021-11-04 | 2021-12-31 | 河南工业大学 | Method for extracting peanut oil body and protein by salt ion assisted aqueous enzymatic method |
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| CN115990442A (en) * | 2023-02-10 | 2023-04-21 | 华南农业大学 | Tea oil microcapsule with double-layer core-shell structure and preparation method and application thereof |
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