CN103484237A - Enzymatic pretreatment method for squeezing evening primrose oil - Google Patents
Enzymatic pretreatment method for squeezing evening primrose oil Download PDFInfo
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- CN103484237A CN103484237A CN201310450111.8A CN201310450111A CN103484237A CN 103484237 A CN103484237 A CN 103484237A CN 201310450111 A CN201310450111 A CN 201310450111A CN 103484237 A CN103484237 A CN 103484237A
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Abstract
The invention provides an enzymatic pretreatment method for squeezing evening primrose oil, and solves the problems that the existing squeezing method has the defects that the oil yield is low, equipment is seriously worn, and the evening primrose oil is poor in quality. The enzymatic pretreatment method for squeezing the evening primrose oil comprises the following steps: firstly, pre-treating evening primrose seeds with an enzyme; secondly, conducting centrifugal separation on a product to obtain free oil and an oily material; finally, putting the oily material of which the water content is regulated into a squeezer, and squeezing to obtain the evening primrose oil. Due to sufficient enzymatic pretreatment on the evening primrose seeds, part of high-quality oil is collected during the process, and the cell walls of the evening primrose seeds can be thoroughly damaged, thus reducing the further squeezing difficulty; meanwhile the squeezing temperature is reduced to avoid deterioration of the evening primrose oil at a high temperature, so that gamma-linolenic acid, linoleic acid and other unsaturated acids in the evening primrose oil are well protected and the obtained oil is perfect in quality.
Description
Technical field
The present invention relates to a kind of method by enzyme process pre-treatment squeezing Oenothera oil.
Background technology
Contain 20～30% Oenothera oil in seed of Radix Oenotherae erythrosepalae, in Oenothera oil, gamma-linolenic acid content is 9 ~ 12%, but gamma-linolenic acid synthesis of prostaglandins E
1, it is antithrombotic, expands the blood vessel new drug, is widely used in thromboangiitis, chronic arteria occlusion disease, myocardial infarction, central vein of retina thrombus angiography, reconstructing blood vessel radiography etc.Research shows that the effect of gamma-linolenic acid reduction cholesterol is linoleic 170 times, and it also can eliminate oxyradical in addition, delaying skin structure organization aging, the active function of reinforcement skin.But human body can not synthesize gamma-linolenic acid, can only absorb by diet, and Oenothera oil is the natural best resource containing gamma-linolenic acid.
At present the extraction of Oenothera oil adopted to milling process more, yet the milling process oil yield efficiency is low, and equipment attrition is serious, the Oenothera oil inferior quality.This invention adopts the method for squeezing again after the enzyme process pre-treatment; seed of Radix Oenotherae erythrosepalae is carried out to sufficient enzymolysis pre-treatment; collect part branded oil fat in this process; can fully destroy the root of Redsepal Eveningprimrose cell walls again, reduce the difficulty of further squeezing, also reduce pressing temperature simultaneously; avoid Oenothera oil at high temperature rotten; protected well the unsaturated acid such as gamma-linolenic acid in the Oenothera oil and linolic acid, the oil quality perfection that obtains, provide reference for realizing that the Oenothera oil industrialization is produced.
Summary of the invention
The present invention is low in order to solve in existing pressing method oil yield, and equipment attrition is serious, the problems such as Oenothera oil inferior quality, and a kind of method by enzyme process pre-treatment squeezing Oenothera oil has been proposed.By the method for enzyme process pre-treatment squeezing Oenothera oil, by following steps, realize: one, enzyme process pre-treatment seed of Radix Oenotherae erythrosepalae; Two, the enzyme that goes out; Three, centrifugation; Four, oil-containing material pre-treatment; Five, squeezing oil-containing material.
Utilize in enzyme process pre-treatment squeezing Oenothera oil; seed of Radix Oenotherae erythrosepalae is carried out to sufficient enzymolysis pre-treatment; collect part branded oil fat in this process; can fully destroy the root of Redsepal Eveningprimrose cell walls again; reduced the difficulty of further squeezing; also reduce pressing temperature simultaneously and avoid Oenothera oil at high temperature rotten, protected well the unsaturated acid such as gamma-linolenic acid in the Oenothera oil and linolic acid, the oil quality perfection that obtains.
Embodiment
Embodiment one: a kind of method by enzyme process pre-treatment squeezing Oenothera oil realizes by following steps:
Step 1: enzyme process pre-treatment seed of Radix Oenotherae erythrosepalae: pulverize after the seed of Radix Oenotherae erythrosepalae cleaning, cross 40 mesh sieves, then spray uniformly upper complex polysaccharide enzyme, plant hydrolyzed enzyme, cellulase, enzymolysis seed of Radix Oenotherae erythrosepalae under the condition of 40 ~ 60 ℃ of temperature, pH value 4.0 ~ 6.5, enzyme dosage 0.5 ~ 3.5%, enzymolysis time 4.0 ~ 6.5h;
Step 2: enzyme goes out: enzyme 3min goes out under 100 ℃;
Step 3: centrifugation: product is put into to whizzer, separate and obtain free oil and oil-containing material;
Step 4: oil-containing material pre-treatment: after oven dry, vacuum conditions moisture;
Step 5: squeezing oil-containing material: the oil-containing material of having reconciled moisture is put into to squeezing machine, squeezing oil content material under the condition that initially enters to press 30 ~ 90 ℃ of temperature, 50 ~ 120 ℃ of pressing temperatures, squeeze pressure 2.0 ~ 6.0MPa.
Embodiment two: the difference of present embodiment and embodiment one is that complex polysaccharide enzyme in step 1, plant hydrolyzed enzyme, cellulase add oil plant in the ratio of 4:1:1, solid-liquid ratio 1:6.Other steps are identical with embodiment one.
Embodiment three: the difference of present embodiment and embodiment one is enzymolysis seed of Radix Oenotherae erythrosepalae under the condition of 45 ~ 55 ℃ of hydrolysis temperatures, pH value 4.5 ~ 5.5, enzyme dosage 1 ~ 3%, enzymolysis time 3.5 ~ 5.5h in step 1.Other steps are identical with embodiment one.
Embodiment four: the difference of present embodiment and embodiment one is in step 4 to regulate material moisture 5 ~ 10%.Other steps are identical with embodiment one.
Embodiment five: the difference of present embodiment and embodiment one is in step 5 initially to enter to press 40 ~ 80 ℃ of temperature, 60 ~ 100 ℃ of pressing temperatures, and squeeze pressure 3.0 ~ 5.0MPa, oil yield is 69%.Other steps are identical with embodiment one.
Claims (5)
1. the method by enzyme process pre-treatment squeezing Oenothera oil is characterized in that with the method steps of enzyme process pre-treatment squeezing Oenothera oil as follows:
Step 1: enzyme process pre-treatment seed of Radix Oenotherae erythrosepalae: enzyme process pre-treatment seed of Radix Oenotherae erythrosepalae: pulverize after the seed of Radix Oenotherae erythrosepalae cleaning, cross 40 mesh sieves, then spray uniformly upper complex polysaccharide enzyme, plant hydrolyzed enzyme, cellulase, enzymolysis seed of Radix Oenotherae erythrosepalae under the condition of 40 ~ 60 ℃ of temperature, pH value 4.0 ~ 6.5, enzyme dosage 0.5 ~ 3.5%, enzymolysis time 4.0 ~ 6.5h;
Step 2: enzyme goes out: enzyme 3min goes out under 100 ℃;
Step 3: centrifugation: product is put into to whizzer, separate and obtain free oil and oil-containing material;
Step 4: oil-containing material pre-treatment: after oven dry, vacuum conditions moisture;
Step 5: squeezing oil-containing material: the oil-containing material of having reconciled moisture is put into to squeezing machine, squeezing oil content material under the condition that initially enters to press 30 ~ 90 ℃ of temperature, 50 ~ 120 ℃ of pressing temperatures, squeeze pressure 2.0 ~ 6.0MPa.
2. a kind of method by enzyme process pre-treatment squeezing Oenothera oil according to claim 1, is characterized in that complex polysaccharide enzyme in step 1, plant hydrolyzed enzyme, cellulase add oil plant in the ratio of 4:1:1, solid-liquid ratio 1:6.
3. according to a kind of method by enzyme process pre-treatment squeezing Oenothera oil claimed in claim 1, it is characterized in that enzymolysis seed of Radix Oenotherae erythrosepalae under the condition of 45 ~ 55 ℃ of hydrolysis temperatures, pH value 4.5 ~ 5.5, enzyme dosage 1 ~ 3%, enzymolysis time 3.5 ~ 5.5h in step 1.
4. according to a kind of method by enzyme process pre-treatment squeezing Oenothera oil claimed in claim 1, it is characterized in that step 4 kind adjusting material moisture 5 ~ 10%.
5. according to a kind of method by enzyme process pre-treatment squeezing Oenothera oil claimed in claim 1, it is characterized in that in step 5 initially entering to press 40 ~ 80 ℃ of temperature, 60 ~ 100 ℃ of pressing temperatures, squeeze pressure 3.0 ~ 5.0MPa, oil yield is 69%.
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| CN201310450111.8A CN103484237A (en) | 2013-09-29 | 2013-09-29 | Enzymatic pretreatment method for squeezing evening primrose oil |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104257523A (en) * | 2014-09-15 | 2015-01-07 | 吉林市圣基实业有限公司 | Moisturizing emulsion containing evening primrose seed oil and preparation method of moisturizing emulsion |
| CN106929147A (en) * | 2017-03-10 | 2017-07-07 | 河南工业大学 | A kind of method for preparing oil body |
| CN107057834A (en) * | 2017-04-16 | 2017-08-18 | 刘清 | The preparation technology of health-care peanut oil |
| CN112920891A (en) * | 2021-03-05 | 2021-06-08 | 河北宁心机械设计有限公司 | Virgin peanut oil and production process thereof |
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|---|---|---|---|---|
| CN104257523A (en) * | 2014-09-15 | 2015-01-07 | 吉林市圣基实业有限公司 | Moisturizing emulsion containing evening primrose seed oil and preparation method of moisturizing emulsion |
| CN106929147A (en) * | 2017-03-10 | 2017-07-07 | 河南工业大学 | A kind of method for preparing oil body |
| CN107057834A (en) * | 2017-04-16 | 2017-08-18 | 刘清 | The preparation technology of health-care peanut oil |
| CN112920891A (en) * | 2021-03-05 | 2021-06-08 | 河北宁心机械设计有限公司 | Virgin peanut oil and production process thereof |
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