CN103484237A - Enzymatic pretreatment method for squeezing evening primrose oil - Google Patents
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- 235000008524 evening primrose extract Nutrition 0.000 title claims abstract description 28
- 229940089020 evening primrose oil Drugs 0.000 title claims abstract description 28
- 239000010475 evening primrose oil Substances 0.000 title claims abstract description 28
- 230000002255 enzymatic effect Effects 0.000 title claims abstract description 15
- 238000002203 pretreatment Methods 0.000 title 1
- 238000003825 pressing Methods 0.000 claims abstract description 29
- 239000003921 oil Substances 0.000 claims abstract description 18
- 241000219925 Oenothera Species 0.000 claims abstract description 17
- 235000004496 Oenothera biennis Nutrition 0.000 claims abstract description 17
- 108090000790 Enzymes Proteins 0.000 claims abstract description 16
- 102000004190 Enzymes Human genes 0.000 claims abstract description 16
- 239000000463 material Substances 0.000 claims abstract description 16
- 238000000034 method Methods 0.000 claims abstract description 16
- 229940088598 enzyme Drugs 0.000 claims description 15
- 241000196324 Embryophyta Species 0.000 claims description 5
- 230000002779 inactivation Effects 0.000 claims description 5
- 108010059892 Cellulase Proteins 0.000 claims description 4
- 229940106157 cellulase Drugs 0.000 claims description 4
- -1 compound polysaccharide Chemical class 0.000 claims description 4
- 230000007071 enzymatic hydrolysis Effects 0.000 claims description 4
- 238000006047 enzymatic hydrolysis reaction Methods 0.000 claims description 4
- 229920001282 polysaccharide Polymers 0.000 claims description 4
- 239000005017 polysaccharide Substances 0.000 claims description 4
- 238000000926 separation method Methods 0.000 claims description 3
- 108090000604 Hydrolases Proteins 0.000 claims description 2
- 238000001035 drying Methods 0.000 claims description 2
- 230000003301 hydrolyzing effect Effects 0.000 claims description 2
- 239000007788 liquid Substances 0.000 claims description 2
- 239000007787 solid Substances 0.000 claims 1
- VZCCETWTMQHEPK-UHFFFAOYSA-N gamma-Linolensaeure Natural products CCCCCC=CCC=CCC=CCCCCC(O)=O VZCCETWTMQHEPK-UHFFFAOYSA-N 0.000 abstract description 11
- VZCCETWTMQHEPK-QNEBEIHSSA-N gamma-linolenic acid Chemical compound CCCCC\C=C/C\C=C/C\C=C/CCCCC(O)=O VZCCETWTMQHEPK-QNEBEIHSSA-N 0.000 abstract description 8
- 235000020664 gamma-linolenic acid Nutrition 0.000 abstract description 8
- 229960002733 gamolenic acid Drugs 0.000 abstract description 8
- OYHQOLUKZRVURQ-HZJYTTRNSA-N Linoleic acid Chemical compound CCCCC\C=C/C\C=C/CCCCCCCC(O)=O OYHQOLUKZRVURQ-HZJYTTRNSA-N 0.000 abstract description 4
- 235000020778 linoleic acid Nutrition 0.000 abstract description 4
- 239000002253 acid Substances 0.000 abstract description 3
- 150000007513 acids Chemical class 0.000 abstract description 3
- 210000002421 cell wall Anatomy 0.000 abstract description 3
- 229940098330 gamma linoleic acid Drugs 0.000 abstract description 3
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 2
- 238000006911 enzymatic reaction Methods 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- 208000031104 Arterial Occlusive disease Diseases 0.000 description 1
- 208000018262 Peripheral vascular disease Diseases 0.000 description 1
- 206010038908 Retinal vein thrombosis Diseases 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 238000002583 angiography Methods 0.000 description 1
- 230000002785 anti-thrombosis Effects 0.000 description 1
- 239000003146 anticoagulant agent Substances 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 235000012000 cholesterol Nutrition 0.000 description 1
- 230000001684 chronic effect Effects 0.000 description 1
- 230000006866 deterioration Effects 0.000 description 1
- 230000002542 deteriorative effect Effects 0.000 description 1
- 235000005911 diet Nutrition 0.000 description 1
- 230000037213 diet Effects 0.000 description 1
- 238000003384 imaging method Methods 0.000 description 1
- 238000009776 industrial production Methods 0.000 description 1
- OYHQOLUKZRVURQ-IXWMQOLASA-N linoleic acid Natural products CCCCC\C=C/C\C=C\CCCCCCCC(O)=O OYHQOLUKZRVURQ-IXWMQOLASA-N 0.000 description 1
- 208000010125 myocardial infarction Diseases 0.000 description 1
- 239000002547 new drug Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- GMVPRGQOIOIIMI-DWKJAMRDSA-N prostaglandin E1 Chemical compound CCCCC[C@H](O)\C=C\[C@H]1[C@H](O)CC(=O)[C@@H]1CCCCCCC(O)=O GMVPRGQOIOIIMI-DWKJAMRDSA-N 0.000 description 1
- 210000001957 retinal vein Anatomy 0.000 description 1
- 208000004644 retinal vein occlusion Diseases 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
- 230000002792 vascular Effects 0.000 description 1
- 229940124549 vasodilator Drugs 0.000 description 1
- 239000003071 vasodilator agent Substances 0.000 description 1
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- Medicines Containing Plant Substances (AREA)
Abstract
本发明提供一种酶法预处理压榨月见草油的方法。它解决了现有压榨方法出油率低，设备磨损严重，月见草油品质较差等问题。其发明步骤如下：首先用酶预处理月见草籽，将产物离心分离可以得到游离油和含油物料，然后将调节好水分的含油物料放入压榨机中压榨得到月见草油。由于对月见草籽进行充分的酶解前处理，在此过程中收集部分优质油脂，又能充分破坏月见草细胞壁，降低了进一步压榨的难度，同时也降低了压榨温度避免月见草油在高温下变质，很好地保护了月见草油中的γ-亚麻酸以及亚油酸等不饱和酸，所获油脂品质完美。The invention provides a method for enzymatically pretreating and pressing evening primrose oil. It solves the problems of low oil yield, serious equipment wear and poor quality of evening primrose oil in the existing pressing method. The inventive steps are as follows: first, pretreat evening primrose seeds with enzymes, centrifuge the product to obtain free oil and oily materials, and then put the oily materials with adjusted moisture into a press to obtain evening primrose oil. Due to the sufficient enzymatic pretreatment of evening primrose seeds, part of the high-quality oil is collected in the process, and the cell wall of evening primrose can be fully destroyed, which reduces the difficulty of further pressing, and also reduces the pressing temperature to avoid evening primrose oil. It deteriorates at high temperature, and the unsaturated acids such as gamma-linolenic acid and linoleic acid in evening primrose oil are well protected, and the quality of the obtained oil is perfect.
Description
技术领域 technical field
本发明涉及一种用酶法预处理压榨月见草油的方法。 The invention relates to a method for pretreating pressed evening primrose oil by enzymatic method.
背景技术 Background technique
月见草籽中含有20～30%的月见草油，月见草油中γ-亚麻酸含量为9~12%，γ-亚麻酸可合成前列腺素E1，它是抗血栓、扩血管新药，广泛用于血栓性脉管炎、慢性动脉闭塞症、心肌梗塞、视网膜中央静脉血栓动脉造影、血管重建造影等。研究表明γ-亚麻酸降低胆固醇的效果是亚油酸的170倍，另外其还可消除氧自由基，延缓皮肤结构组织衰老，加强皮肤的活性功能。但是人体不能合成γ-亚麻酸，只能通过饮食来摄取，而月见草油是天然的含γ-亚麻酸的最好资源。 Evening primrose seeds contain 20-30% evening primrose oil, the content of gamma-linolenic acid in evening primrose oil is 9-12%, gamma-linolenic acid can synthesize prostaglandin E 1 , which is antithrombotic, vasodilator New drug, widely used in thromboangiitis, chronic arterial occlusive disease, myocardial infarction, central retinal vein thrombosis arteriography, vascular reconstruction imaging, etc. Studies have shown that γ-linolenic acid is 170 times more effective in lowering cholesterol than linoleic acid. In addition, it can also eliminate oxygen free radicals, delay the aging of skin structure and tissue, and strengthen the active function of skin. However, the human body cannot synthesize γ-linolenic acid and can only be ingested through diet. Evening primrose oil is the best natural source of γ-linolenic acid.
目前对月见草油的提取多采用压榨法，然而压榨法出油效率低，且设备磨损严重，月见草油品质较差。该发明采用酶法预处理后再压榨的方法，即对月见草籽进行充分的酶解前处理，在此过程中收集部分优质油脂，又能充分破坏月见草细胞壁，降低了进一步压榨的难度，同时也降低了压榨温度，避免月见草油在高温下变质，很好地保护了月见草油中的γ-亚麻酸以及亚油酸等不饱和酸，所获油脂品质完美，为实现月见草油工业化制取提供参考。 At present, the extraction of evening primrose oil is mostly carried out by pressing, but the oil extraction efficiency of the pressing method is low, and the equipment wears seriously, so the quality of evening primrose oil is poor. The invention adopts the method of enzymatic pretreatment and then squeezing, that is, the evening primrose seeds are fully enzymatically pretreated, and part of the high-quality oil is collected in the process, which can fully destroy the cell wall of the evening primrose and reduce the cost of further squeezing. difficulty, and at the same time reduce the pressing temperature, avoid the deterioration of evening primrose oil at high temperature, well protect the unsaturated acids such as gamma-linolenic acid and linoleic acid in evening primrose oil, and the quality of the obtained oil is perfect, for Provide reference for the industrial production of evening primrose oil.
发明内容 Contents of the invention
本发明为了解决现有压榨方法中出油率低，设备磨损严重，月见草油品质较差等问题，而提出了一种用酶法预处理压榨月见草油的方法。用酶法预处理压榨月见草油的方法通过以下步骤实现：一、酶法预处理月见草籽；二、灭酶；三、离心分离；四、含油物料预处理；五、压榨含油物料。 In order to solve the problems of low oil yield, serious equipment wear and poor quality of evening primrose oil in the existing pressing method, the present invention proposes a method for pretreatment and pressing of evening primrose oil by enzymatic method. The method for pressing evening primrose oil by enzymatic pretreatment is realized through the following steps: 1. Enzymatic pretreatment of evening primrose seeds; 2. Enzyme inactivation; 3. Centrifugal separation; 4. Pretreatment of oily materials; .
利用酶法预处理压榨月见草油中，对月见草籽进行充分的酶解前处理，在此过程中收集部分优质油脂，又能充分破坏月见草细胞壁，降低了进一步压榨的难度，同时也降低了压榨温度避免月见草油在高温下变质，很好地保护了月见草油中的γ-亚麻酸以及亚油酸等不饱和酸，所获油脂品质完美。 Enzymatic pretreatment is used to squeeze evening primrose oil, and the evening primrose seeds are fully enzymatically pretreated. During this process, part of the high-quality oil is collected, and the cell wall of evening primrose can be fully destroyed, reducing the difficulty of further pressing. At the same time, the pressing temperature is also reduced to prevent the evening primrose oil from deteriorating at high temperature, and the unsaturated acids such as gamma-linolenic acid and linoleic acid in the evening primrose oil are well protected, and the quality of the obtained oil is perfect.
具体实施方式 Detailed ways
具体实施方式一：一种用酶法预处理压榨月见草油的方法通过以下步骤实现： Specific embodiment one: a kind of method of squeezing evening primrose oil with enzymatic pretreatment realizes through the following steps:
步骤一：酶法预处理月见草籽：月见草籽清理后粉碎，过40目筛，然后均匀的喷洒上复合多糖酶、植物水解酶、纤维素酶，在温度40~60℃、pH值4.0~6.5、酶用量0.5~3.5%、酶解时间4.0~6.5h的条件下酶解月见草籽； Step 1: Enzymatic pretreatment of evening primrose seeds: Clean evening primrose seeds, crush them, pass through a 40-mesh sieve, and then evenly spray compound polysaccharide enzymes, plant hydrolytic enzymes, and cellulase at a temperature of 40~60°C and pH Enzymolysis of evening primrose seeds under the conditions of 4.0~6.5 value, 0.5~3.5% enzyme dosage, and 4.0~6.5h enzymolysis time;
步骤二：灭酶：100℃下灭酶3min； Step 2: Enzyme inactivation: Enzyme inactivation at 100°C for 3 minutes;
步骤三：离心分离：将产物放入离心机中，分离得到游离油和含油物料； Step 3: Centrifugal separation: put the product into a centrifuge to separate free oil and oily materials;
步骤四：含油物料预处理：烘干后，真空调节水分； Step 4: Pretreatment of oil-containing materials: after drying, vacuum to adjust the moisture;
步骤五：压榨含油物料：将调解好水分的含油物料放入压榨机中，在初始入榨温度30~90℃、压榨温度50~120℃、压榨压力2.0~6.0MPa的条件下压榨含油物料。 Step 5: Squeeze the oily material: Put the oily material with adjusted moisture into the press, and press the oily material under the conditions of the initial pressing temperature of 30-90°C, pressing temperature of 50-120°C, and pressing pressure of 2.0-6.0MPa.
具体实施方式二：本实施方式与具体实施方式一的不同点在于步骤一中复合多糖酶、植物水解酶、纤维素酶按4：1：1的比例加入油料，料液比1：6。其他步骤与具体实施方式一相同。 Embodiment 2: The difference between this embodiment and Embodiment 1 is that in step 1, compound polysaccharide enzyme, plant hydrolase, and cellulase are added to the oil plant in a ratio of 4:1:1, and the ratio of material to liquid is 1:6. Other steps are the same as in the first embodiment.
具体实施方式三：本实施方式与具体实施方式一的不同点在于步骤一中酶解温度45~55℃、pH值4.5~5.5、酶用量1~3%、酶解时间3.5~5.5h的条件下酶解月见草籽。其他步骤与具体实施方式一相同。 Specific embodiment 3: The difference between this embodiment and specific embodiment 1 lies in the conditions in step 1 that the enzymolysis temperature is 45-55°C, the pH value is 4.5-5.5, the enzyme dosage is 1-3%, and the enzymolysis time is 3.5-5.5h Enzymatic hydrolysis of evening primrose seeds. Other steps are the same as in the first embodiment.
具体实施方式四：本实施方式与具体实施方式一的不同点在于步骤四中调节物料水分5~10%。其他步骤与具体实施方式一相同。 Embodiment 4: The difference between this embodiment and Embodiment 1 is that the moisture content of the material is adjusted to 5-10% in step 4. Other steps are the same as in the first embodiment.
具体实施方式五：本实施方式与具体实施方式一的不同点在于步骤五中初始入榨温度40~80℃，压榨温度60~100℃，压榨压力3.0~5.0MPa，出油率为69%。其他步骤与具体实施方式一相同。 Embodiment 5: The difference between this embodiment and Embodiment 1 is that in step 5, the initial pressing temperature is 40-80°C, the pressing temperature is 60-100°C, the pressing pressure is 3.0-5.0 MPa, and the oil yield is 69%. Other steps are the same as in the first embodiment.
Claims (5)
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104257523A (en) * | 2014-09-15 | 2015-01-07 | 吉林市圣基实业有限公司 | Moisturizing emulsion containing evening primrose seed oil and preparation method of moisturizing emulsion |
| CN106929147A (en) * | 2017-03-10 | 2017-07-07 | 河南工业大学 | A kind of method for preparing oil body |
| CN107057834A (en) * | 2017-04-16 | 2017-08-18 | 刘清 | The preparation technology of health-care peanut oil |
| CN112920891A (en) * | 2021-03-05 | 2021-06-08 | 河北宁心机械设计有限公司 | Virgin peanut oil and production process thereof |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1935962A (en) * | 2006-10-18 | 2007-03-28 | 江南大学 | Method for extracting linseed oil by water-enzyme process |
| CN101235399A (en) * | 2008-03-05 | 2008-08-06 | 中国林业科学研究院亚热带林业研究所 | Enzymatic Extraction Technology of Oil from Camellia Camellia Seed Kernel |
| CN101779705A (en) * | 2010-02-22 | 2010-07-21 | 北京市科威华食品工程技术有限公司 | Production method of grape seed oil |
| CN102100261A (en) * | 2010-12-30 | 2011-06-22 | 赵群发 | Cold-pressed unsaturated fatty acid apricot kernel oil, and production method and application thereof |
| CN102181319A (en) * | 2011-04-06 | 2011-09-14 | 贵州大学 | Oil making method based on lower-moisture enzymatic auxiliary cold pressing |
| CN103060078A (en) * | 2011-10-20 | 2013-04-24 | 中国科学院兰州化学物理研究所 | Method for aqueous enzymatic extraction of gardenia oil |
-
2013
- 2013-09-29 CN CN201310450111.8A patent/CN103484237A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1935962A (en) * | 2006-10-18 | 2007-03-28 | 江南大学 | Method for extracting linseed oil by water-enzyme process |
| CN101235399A (en) * | 2008-03-05 | 2008-08-06 | 中国林业科学研究院亚热带林业研究所 | Enzymatic Extraction Technology of Oil from Camellia Camellia Seed Kernel |
| CN101779705A (en) * | 2010-02-22 | 2010-07-21 | 北京市科威华食品工程技术有限公司 | Production method of grape seed oil |
| CN102100261A (en) * | 2010-12-30 | 2011-06-22 | 赵群发 | Cold-pressed unsaturated fatty acid apricot kernel oil, and production method and application thereof |
| CN102181319A (en) * | 2011-04-06 | 2011-09-14 | 贵州大学 | Oil making method based on lower-moisture enzymatic auxiliary cold pressing |
| CN103060078A (en) * | 2011-10-20 | 2013-04-24 | 中国科学院兰州化学物理研究所 | Method for aqueous enzymatic extraction of gardenia oil |
Non-Patent Citations (1)
| Title |
|---|
| 鲁志成: ""植物油脂制取工艺过程中的水分控制"", 《粮食加工》, no. 3, 31 December 2004 (2004-12-31), pages 21 - 24 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104257523A (en) * | 2014-09-15 | 2015-01-07 | 吉林市圣基实业有限公司 | Moisturizing emulsion containing evening primrose seed oil and preparation method of moisturizing emulsion |
| CN106929147A (en) * | 2017-03-10 | 2017-07-07 | 河南工业大学 | A kind of method for preparing oil body |
| CN107057834A (en) * | 2017-04-16 | 2017-08-18 | 刘清 | The preparation technology of health-care peanut oil |
| CN112920891A (en) * | 2021-03-05 | 2021-06-08 | 河北宁心机械设计有限公司 | Virgin peanut oil and production process thereof |
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