CN106190524A - A kind of production method of aqueous enzymatic extraction Oleum Camelliae - Google Patents
A kind of production method of aqueous enzymatic extraction Oleum Camelliae Download PDFInfo
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- CN106190524A CN106190524A CN201610687314.2A CN201610687314A CN106190524A CN 106190524 A CN106190524 A CN 106190524A CN 201610687314 A CN201610687314 A CN 201610687314A CN 106190524 A CN106190524 A CN 106190524A
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- CN
- China
- Prior art keywords
- oleum camelliae
- production method
- extraction
- camellia oleosa
- oleosa seed
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Classifications
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B1/00—Production of fats or fatty oils from raw materials
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B3/00—Refining fats or fatty oils
Abstract
The invention discloses a kind of water production method for Enzymatic Extraction Oleum Camelliae, by under suitable pH, add mixed enzyme, mechanical shaking extraction is carried out in glacial acetic acid sodium chloride solution, utilizing enzymolysis and sodium chloride solution to form osmotic pressure makes the Oleum Camelliae in camellia oleosa seed quickly discharge, Oleum Camelliae is carried out the yield that sterilizes again, after being dried, obtains product Oleum Camelliae；Camellia oleosa seed oil extracting rate of the present invention is high, and in Oleum Camelliae, content of microorganisms is up to standard, and organic solvent-free remains, and is suitable for large-scale promotion.
Description
Technical field
The present invention relates to edible oil manufacture field, be specifically related to the production method of a kind of aqueous enzymatic extraction Oleum Camelliae.
Background technology
Oleum Camelliae obtains from Theaceae tea oil tree seed, has another name called Oleum Camelliae, Camellia oil, is the distinctive woody oil of China
Fat.China's oil tea aboundresources, camellia oleifera lam area accounts for more than 80% that woody edible oil charge level is long-pending, is distributed widely in south 17
The knob of individual province, especially with Guangxi, Hunan, Jiangxi, the ground such as Yunnan be most.
Containing several functions composition in Oleum Camelliae, for maintaining the function of cardiovascular system, improve body immunity, reduce
Cholesterol, prevents and treats hypertension have obvious effect.Additionally, traditional medicine is thought, Oleum Camelliae has clearing away heat and eliminating dampness, parasite killing to detoxify
Effect, can clearing stomach intestine moistening, eruptive disease stomachache, acute ascarid obstructive intestinal obstruction etc. can be controlled.
The extraction of Oleum Camelliae has milling process, organic solvent extraction, water substitution and aqueous enzymatic method etc..At present, commercial production is commonly used
Method be mainly milling process, organic solvent extraction.Milling process technique is simple, but press apparatus maintenance changes the amount of labour greatly,
Extraction efficiency is relatively low；Organic solvent extraction efficiency is the highest, and production cost is relatively low, but organic reagent exists potential safety and asks
Topic；It is simple that water substitution produces equipment, processing ease, and cost is relatively low, but its oil yield is low, and the acid value of lipids proposed is high, uses water
Measure bigger.
Chinese patent (application number: CN201210033352.8, denomination of invention: a kind of ultrasonic auxiliary Enzymatic Extraction Oleum Camelliae
Production method, the applying date: on February 15th, 2012), disclose the production method of a kind of ultrasonic auxiliary Enzymatic Extraction Oleum Camelliae, pass through
Adjusting and add alkaline protease and compound cellulase after pH and carry out enzymolysis to the Oleum Camelliae extracting in camellia oleosa seed, oil yield is high, but
This process comprises twice regulation pH, adds enzyme twice and carrys out enzymolysis, and extraction efficiency is the highest, easily has enzyme to remain, cause in Oleum Camelliae
Sanitary index is the most up to standard,
Document " technique that water substitution extracts Oleum Camelliae from Semen Camelliae " (Transactions of the Chinese Society of Agricultural Engineering, the 9th phase of volume 24,2008 years 9
Month, Guo Yubao etc.) disclose a kind of method that water substitution extracts Oleum Camelliae from tea seed, optimum condition is, dries with decortication
Camellia oleosa seed is raw material, is 75 DEG C in temperature, and extraction time is 150min, and ratio of water to material is 4.5:1 (mL/g) and the condition of pH value 9.0
Lower extraction, Oleum Camelliae extraction rate is up to 80.28%.Extraction ratio is higher than general water substitution, but tea certain in residual in Oleum Camelliae
Saponin, remains more Oleum Camelliae in the screenings obtained, comprehensive utilization ratio is the highest.
Summary of the invention
For overcoming extracting method extraction ratio in existing Oleum Camelliae low, equipment requirements is high, operation complexity, and in Oleum Camelliae, microorganism surpasses
Mark, the deficiency such as tea saponin residual, the present invention provides a kind of water the most easily to grasp for the production method of Enzymatic Extraction Oleum Camelliae, the method
Work, oil extracting rate are high, and low cost, microbiological indicator is up to standard.
The present invention is achieved by the following technical programs:
A kind of water, for the production method of Enzymatic Extraction Oleum Camelliae, comprises the steps of
1) get the raw materials ready
Choose the camellia oleosa seed dried, shelling, pulverize stand-by；
2) pH and extraction are adjusted
Add water, sodium chloride, regulate pH with glacial acetic acid, add mixed enzyme, extract；
3) sterilization
By step 2) in material, under ultra violet lamp sterilize；
4) it is layered and is dried
Stratification, takes out upper strata oil reservoir, is vacuum dried, obtains finished product Oleum Camelliae.
Further, described step 1) in, pulverizing requirement is: be ground into the camellia oleosa seed powder of 100-130 mesh.
Further, described step 2) in, pH regulator to 4.7-5.5.
Further, described step 2) in, mixed enzyme is made up of protease and cellulase, is to be 1 according to mass ratio:
1 is obtained by mixing.
Further, described step 2) in, extraction conditions is: extracting in mechanical shaking extraction instrument, temperature is 30-39 DEG C, carries
Taking instrument rotating speed 100r/min, the time is 5-6 hour.
Further, described step 3) in, under ultra violet lamp, sterilizing time is 2-3 hour.
Further, described step 1)-2) in, camellia oleosa seed, water, sodium chloride, the addition of mixed enzyme, according to weight ratio, camellia oleosa seed:
Water: sodium chloride: mixed enzyme=1:4-5:0.1-0.2:0.06-0.1.
The present invention is that the mixed enzyme of addition, sodium chloride carry out mechanical shaking extraction, are added by enzymolysis, utilization by under suitable pH
Oleum Camelliae in camellia oleosa seed is quickly discharged by the infiltration pressure energy entering sodium chloride formation, is simultaneously introduced sodium chloride and contributes to abolishing breast
Change layer, improve oil yield；By introducing sterilisation step, go out except mixed enzyme and the bacterium that disappears, it is ensured that in Oleum Camelliae, content of microorganisms is qualified.
The invention has the beneficial effects as follows: the Oleum Camelliae extraction method of technical solution of the present invention, simple to operation, equipment requirements
Low, camellia oleosa seed oil extracting rate is high, and in Oleum Camelliae, microorganism is up to standard, and organic solvent-free remains, and is suitable for large-scale promotion.
Detailed description of the invention
Below in conjunction with embodiment, technical scheme is further described, but claimed scope is not limited to
In described.
Embodiment 1
1) get the raw materials ready
Choose the camellia oleosa seed dried, shelling, pulverize stand-by；Choose the camellia oleosa seed drying (moisture Control is below 10%)
100g, shelling, camellia oleosa seed is pulverized, crosses the sieve of 100 mesh, obtain camellia oleosa seed powder, stand-by；
2) pH and extraction are adjusted
In mechanical shaking extraction instrument add step 1) in camellia oleosa seed powder, water 400g, sodium chloride 10g, regulate pH with glacial acetic acid
To 5, add mixed enzyme 6g (protease: cellulase=1:1), mechanical shaking extraction instrument rotating speed be 100r/min, temperature be 30-39
Extract 5 hours under conditions of DEG C；
3) sterilization
By step 2) in material, under ultra violet lamp sterilize 2 hours；
4) it is layered and is dried
Stratification, takes out upper strata oil reservoir, is vacuum dried, obtains finished product Oleum Camelliae.
Embodiment 2
1) get the raw materials ready
Choose the camellia oleosa seed dried, shelling, pulverize stand-by；Choose the camellia oleosa seed drying (moisture Control is below 10%)
100g, shelling, camellia oleosa seed is pulverized, crosses the sieve of 130 mesh, obtain camellia oleosa seed powder, stand-by；
2) pH and extraction are adjusted
In mechanical shaking extraction instrument add step 1) in camellia oleosa seed powder, water 500g, sodium chloride 20g, regulate pH with glacial acetic acid
To 5.5, add mixed enzyme 7g (protease: cellulase=1:1), mechanical shaking extraction instrument rotating speed be 100r/min, temperature be 30-
Extract 6 hours under conditions of 39 DEG C；
3) sterilization
By step 2) in material, under ultra violet lamp sterilize 3 hours；
4) it is layered and is dried
Stratification, takes out upper strata oil reservoir, is vacuum dried, obtains finished product Oleum Camelliae.
Embodiment 3
1) get the raw materials ready
Choose the camellia oleosa seed dried, shelling, pulverize stand-by；Choose the camellia oleosa seed drying (moisture Control is below 10%)
100g, shelling, camellia oleosa seed is pulverized, crosses the sieve of 110 mesh, obtain camellia oleosa seed powder, stand-by；
2) pH and extraction are adjusted
In mechanical shaking extraction instrument add step 1) in camellia oleosa seed powder, water 450g, sodium chloride 15g, regulate pH with glacial acetic acid
To 4.8, add mixed enzyme 10g (protease: cellulase=1:1), mechanical shaking extraction instrument rotating speed be 100r/min, temperature be
Extract 5.5 hours under conditions of 30-39 DEG C；
3) sterilization
By step 2) in material, under ultra violet lamp sterilize 2.5 hours；
4) it is layered and is dried
Stratification, takes out upper strata oil reservoir, is vacuum dried, obtains finished product Oleum Camelliae.
Embodiment 4
1) get the raw materials ready
Choose the camellia oleosa seed dried, shelling, pulverize stand-by；Choose the camellia oleosa seed drying (moisture Control is below 10%)
100g, shelling, camellia oleosa seed is pulverized, crosses the sieve of 120 mesh, obtain camellia oleosa seed powder, stand-by；
2) pH and extraction are adjusted
In mechanical shaking extraction instrument add step 1) in camellia oleosa seed powder, water 470g, sodium chloride 17g, regulate pH with glacial acetic acid
To 5.3, add mixed enzyme 9g (protease: cellulase=1:1), mechanical shaking extraction instrument rotating speed be 100r/min, temperature be 30-
Extract 5.7 hours under conditions of 39 DEG C；
3) sterilization
By step 2) in material, under ultra violet lamp sterilize 2.6 hours；
4) it is layered and is dried
Stratification, takes out upper strata oil reservoir, is vacuum dried, obtains finished product Oleum Camelliae.
The finished product Oleum Camelliae preparing embodiment carries out detection and analyzes, and testing result is as shown in the table:
Claims (6)
1. the production method of an aqueous enzymatic extraction Oleum Camelliae, it is characterised in that: described production method, comprise the following steps:
1) get the raw materials ready
Choose the camellia oleosa seed dried, shelling, pulverize stand-by；
2) pH and extraction are adjusted
Add water, sodium chloride, regulate pH with glacial acetic acid, add mixed enzyme, extract；
3) sterilization
By step 2) in material, under ultra violet lamp sterilize；
4) it is layered and is dried
Stratification, takes out upper strata oil reservoir, is vacuum dried, obtains finished product Oleum Camelliae.
A kind of production method of aqueous enzymatic extraction Oleum Camelliae, it is characterised in that: described step 2) in,
PH regulator is to 4.7-5.5.
A kind of production method of aqueous enzymatic extraction Oleum Camelliae, it is characterised in that: described step 2) in,
Mixed enzyme is made up of protease and cellulase, is to be that 1:1 is obtained by mixing according to mass ratio.
A kind of production method of aqueous enzymatic extraction Oleum Camelliae, it is characterised in that: described step 2) in,
Extraction conditions is: extracting in mechanical shaking extraction instrument, temperature is 30-39 DEG C, extraction apparatus rotating speed 100r/min, and the time is 5-6 hour.
A kind of production method of aqueous enzymatic extraction Oleum Camelliae, it is characterised in that: described step 3) in,
Under ultra violet lamp, sterilizing time is 2-3 hour.
A kind of production method of aqueous enzymatic extraction Oleum Camelliae, it is characterised in that: described step 1)-2)
Middle camellia oleosa seed, water, sodium chloride, the addition of mixed enzyme, according to weight ratio, camellia oleosa seed: water: sodium chloride: mixed enzyme=1:4-5:0.1-0.2:
0.06-0.1。
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| Application Number | Priority Date | Filing Date | Title |
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| CN201610687314.2A CN106190524A (en) | 2016-08-18 | 2016-08-18 | A kind of production method of aqueous enzymatic extraction Oleum Camelliae |
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| CN201610687314.2A CN106190524A (en) | 2016-08-18 | 2016-08-18 | A kind of production method of aqueous enzymatic extraction Oleum Camelliae |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106929147A (en) * | 2017-03-10 | 2017-07-07 | 河南工业大学 | A kind of method for preparing oil body |
| CN109054987A (en) * | 2018-08-30 | 2018-12-21 | 广西那坡县翠株园林业科技有限公司 | A kind of production method of tea oil |
| CN114854487A (en) * | 2022-05-26 | 2022-08-05 | 龙岩市祥优实业有限公司 | Extraction process for improving effective medicinal value components of camellia oil |
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| CN1854273A (en) * | 2005-04-26 | 2006-11-01 | 中国科学院沈阳应用生态研究所 | Extraction of hickory nut oil from water enzyme |
| CN103113975A (en) * | 2013-02-05 | 2013-05-22 | 中国林业科学研究院林产化学工业研究所 | Method for extracting tea oil and saccharicterpenin by using physical wall breaking to assist aqueous enzymatic method |
| CN203096025U (en) * | 2013-01-29 | 2013-07-31 | 黄河科技学院 | Post-treatment device of cold-pressed crude oil |
| CN103396879A (en) * | 2013-08-12 | 2013-11-20 | 长沙昊瑞生物科技有限公司 | Demulsification method in production processes for extracting tea-seed oil extraction with participation of water phase |
| CN103525544A (en) * | 2013-09-23 | 2014-01-22 | 华侨大学 | Process for refining tea seed crude oil extracted by aqueous enzymatic method |
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Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1854273A (en) * | 2005-04-26 | 2006-11-01 | 中国科学院沈阳应用生态研究所 | Extraction of hickory nut oil from water enzyme |
| CN203096025U (en) * | 2013-01-29 | 2013-07-31 | 黄河科技学院 | Post-treatment device of cold-pressed crude oil |
| CN103113975A (en) * | 2013-02-05 | 2013-05-22 | 中国林业科学研究院林产化学工业研究所 | Method for extracting tea oil and saccharicterpenin by using physical wall breaking to assist aqueous enzymatic method |
| CN103396879A (en) * | 2013-08-12 | 2013-11-20 | 长沙昊瑞生物科技有限公司 | Demulsification method in production processes for extracting tea-seed oil extraction with participation of water phase |
| CN103525544A (en) * | 2013-09-23 | 2014-01-22 | 华侨大学 | Process for refining tea seed crude oil extracted by aqueous enzymatic method |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106929147A (en) * | 2017-03-10 | 2017-07-07 | 河南工业大学 | A kind of method for preparing oil body |
| CN109054987A (en) * | 2018-08-30 | 2018-12-21 | 广西那坡县翠株园林业科技有限公司 | A kind of production method of tea oil |
| CN114854487A (en) * | 2022-05-26 | 2022-08-05 | 龙岩市祥优实业有限公司 | Extraction process for improving effective medicinal value components of camellia oil |
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