CN103571608A - Method for extracting grape seed oil by utilizing cellulase - Google Patents

Method for extracting grape seed oil by utilizing cellulase Download PDF

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Publication number
CN103571608A
CN103571608A CN201310517488.0A CN201310517488A CN103571608A CN 103571608 A CN103571608 A CN 103571608A CN 201310517488 A CN201310517488 A CN 201310517488A CN 103571608 A CN103571608 A CN 103571608A
Authority
CN
China
Prior art keywords
seed oil
extract
cellulase
grape seed
utilizing
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201310517488.0A
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Chinese (zh)
Inventor
张丽娜
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Priority to CN201310517488.0A priority Critical patent/CN103571608A/en
Priority to PCT/CN2013/001423 priority patent/WO2015061926A1/en
Publication of CN103571608A publication Critical patent/CN103571608A/en
Pending legal-status Critical Current

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Classifications

    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23D—EDIBLE OILS OR FATS, e.g. MARGARINES, SHORTENINGS, COOKING OILS
    • A23D9/00—Other edible oils or fats, e.g. shortenings, cooking oils
    • A23D9/02—Other edible oils or fats, e.g. shortenings, cooking oils characterised by the production or working-up
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
    • C11B1/00—Production of fats or fatty oils from raw materials
    • C11B1/02—Pretreatment
    • C11B1/025—Pretreatment by enzymes or microorganisms, living or dead
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
    • C11B1/00—Production of fats or fatty oils from raw materials
    • C11B1/10—Production of fats or fatty oils from raw materials by extracting

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Fats And Perfumes (AREA)

Abstract

The invention discloses a method for extracting grape seed oil by utilizing cellulose. The method comprises the following steps: removing impurities from grape seeds; drying and crushing the grape seeds into powder; adding a citric acid-sodium citrate solution at a ratio of 1:5 to regulate the pH value to 5-6; heating the solution to 60 DEG C in a water bath and continuing for 10 minutes; adding cellulose and stirring the solution; having enzymolysis reaction for one hour, then adding benzene to extract grape seed oil for 30 minutes; separating the oil phase, evaporating and dehydrating the oil phase, and then obtaining grape seed oil. The method disclosed by the invention not only can recycle waste grape seeds but also can produce high-quality grape seed oil.

Description

A kind of method of utilizing cellulase to extract raisin seed oil
Technical field
The present invention relates to a kind of agricultural byproducts processing method, be specifically related to a kind of method of utilizing cellulase to extract raisin seed oil.
Background technology
The extracting method of raisin seed oil has a lot, there are solvent extration, milling process and supersonic method etc., traditional milling process oil yield is low, acid value, peroxide value, iodine number and moisture and volatile matter content content are all higher, although and supersonic method has solved the problems referred to above, and extraction time is shorter, complicated operation, facility investment is higher, is difficult to suitability for industrialized production.
Summary of the invention
Technical problem to be solved by this invention is for the deficiencies in the prior art, and a kind of method of utilizing cellulase to extract raisin seed oil is provided.
Technical problem to be solved by this invention is to realize by following technical scheme.The present invention is a kind of method of utilizing cellulase to extract raisin seed oil, is characterized in: by Semen Vitis viniferae removal of impurities, dry, be ground into powder, it is 5-6 that the ratio of 1:5 of take adds citric acid-sodium citrate solution to regulate pH value, and heating in water bath to 60 ℃, keeps 10 minutes, add cellulase to stir, enzymolysis time 1 hour, then adds benzene to extract, and extraction time is 30 minutes, isolate oil phase dehydration by evaporation, obtain raisin seed oil.
Compared with prior art, the present invention not only can realize recycling, the higher raisin seed oil of quality that also can make by discarded Semen Vitis viniferae.
Embodiment
Below further describe concrete technical scheme of the present invention, so that those skilled in the art understands the present invention further, and do not form the restriction to its right.
Embodiment 1, and a kind of method of utilizing cellulase to extract raisin seed oil, gets removal of impurities, dry 50 kilograms of Semen Vitis viniferaes, be ground into powder, add 200 kilograms of citric acid-sodium citrate solutions, by concentration adjustment pH value, be 5-6, heating in water bath to 60 ℃, keeps 10 minutes, add 0.2 kilogram of cellulase to stir, enzymolysis time 1 hour, then adds benzene to extract, and extraction time is 30 minutes, isolate oil phase dehydration by evaporation, obtain raisin seed oil.

Claims (4)

1. utilize cellulase to extract a method for raisin seed oil, it is characterized in that raw materials used is Semen Vitis viniferae, through removal of impurities, oven dry, acidolysis, enzymolysis, extraction, separation, evaporation, forms.
2. a kind of method of utilizing cellulase to extract raisin seed oil according to claim 1, is characterized in that described acidolysis is in citric acid-sodium citrate solution, to carry out acidolysis, and acid hydrolysis solution pH value is 5-6, and temperature is 30-80 ℃, and the acidolysis time is 5-30 minute.
3. a kind of method of utilizing cellulase to extract raisin seed oil according to claim 1, is characterized in that described enzymolysis enzyme is cellulase, the 0.05-0.5% that consumption is raw material weight, and enzymolysis time is 0.5-2 hour.
4. a kind of method of utilizing cellulase to extract raisin seed oil according to claim 1, is characterized in that described extraction is to extract with benzene, and extraction time is 10-60 minute.
CN201310517488.0A 2013-10-29 2013-10-29 Method for extracting grape seed oil by utilizing cellulase Pending CN103571608A (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
CN201310517488.0A CN103571608A (en) 2013-10-29 2013-10-29 Method for extracting grape seed oil by utilizing cellulase
PCT/CN2013/001423 WO2015061926A1 (en) 2013-10-29 2013-11-20 A method for extracting grape seed oil by utilizing cellulase

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201310517488.0A CN103571608A (en) 2013-10-29 2013-10-29 Method for extracting grape seed oil by utilizing cellulase

Publications (1)

Publication Number Publication Date
CN103571608A true CN103571608A (en) 2014-02-12

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
CN201310517488.0A Pending CN103571608A (en) 2013-10-29 2013-10-29 Method for extracting grape seed oil by utilizing cellulase

Country Status (2)

Country Link
CN (1) CN103571608A (en)
WO (1) WO2015061926A1 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106929147A (en) * 2017-03-10 2017-07-07 河南工业大学 A kind of method for preparing oil body

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN117099995A (en) * 2023-09-05 2023-11-24 东莞市吉纯生物技术有限公司 Antioxidant electronic atomized liquid and preparation method thereof

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1710042A (en) * 2005-06-17 2005-12-21 长沙桑霖生物科技有限公司 Method for extracting spine grape seed oil from spine grape seeds
CN101779705A (en) * 2010-02-22 2010-07-21 北京市科威华食品工程技术有限公司 Production method of grape seed oil
CN102260587A (en) * 2011-06-11 2011-11-30 威海康柏年药业有限公司 Method for extracting grape seed oil and proantho cyanidins
CN102517144A (en) * 2011-11-28 2012-06-27 宁夏大学 Method for extracting oil and procyanidin from grape seeds

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103193751A (en) * 2013-03-27 2013-07-10 吉首大学 Method for continuously extracting active ingredients from grape seeds

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1710042A (en) * 2005-06-17 2005-12-21 长沙桑霖生物科技有限公司 Method for extracting spine grape seed oil from spine grape seeds
CN101779705A (en) * 2010-02-22 2010-07-21 北京市科威华食品工程技术有限公司 Production method of grape seed oil
CN102260587A (en) * 2011-06-11 2011-11-30 威海康柏年药业有限公司 Method for extracting grape seed oil and proantho cyanidins
CN102517144A (en) * 2011-11-28 2012-06-27 宁夏大学 Method for extracting oil and procyanidin from grape seeds

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
高璐等: "葡萄籽油提取工艺研究及对其理化性质的影响", 《食品科学》, vol. 30, no. 22, 31 December 2009 (2009-12-31), pages 81 - 83 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106929147A (en) * 2017-03-10 2017-07-07 河南工业大学 A kind of method for preparing oil body

Also Published As

Publication number Publication date
WO2015061926A1 (en) 2015-05-07

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Application publication date: 20140212