CN102660619A - Method for clean production of saponin from Dioscorea zingiberensis C. H. Wright - Google Patents

Abstract

The invention discloses a method for clean production of saponin from Dioscorea zingiberensis C. H. Wright. The method comprises the following steps of crushing a dry raw material of Dioscorea zingiberensis C. H. Wright, sieving the powder of Dioscorea zingiberensis C. H. Wright by a sieve of 20 meshes, carrying out methanol or ethanol extraction, carrying out concentration recovery of methanol or ethanol from the extract until an alcohol smell does not exist, putting the extract treated by the previous step into a container, carrying out microbial constant-temperature fermentation, adding hydrochloric acid or sulfuric acid into the container for acid hydrolysis, filtering to obtain crude saponin, carrying out crystallization and recrystallization of the crude saponin by petroleum ether, and carrying out vacuum drying to obtain a finished product. The method for clean production of saponin from Dioscorea zingiberensis C. H. Wright has the advantages of high yield, good color and good quality of saponin, and no pollution.

Description

A kind of method with yellow ginger cleaner production saponin

Technical field

The present invention relates to a kind of method with yellow ginger cleaner production saponin.

Background technology

China diosgenin producer adopts the technology that the direct acid-hydrolysis method of Rothrok initiative is produced diosgenin mostly at present, but directly acid-hydrolysis method only can extract about 1/4 diosgenin.Though through development decades; But because most of saponin manufacturing enterprise small scale, shortage of funding still adopt the traditional technology of " spontaneous fermentation-acidolysis-extractions saponin " to produce saponin at present, though this traditional processing technology operation simple relatively, be easy to production; But there are a lot of problems: in yellow ginger acid hydrolysis process; Because starch and cellulosic interference, saponin can not be by abundant hydrolysis, thereby causes the saponin extraction yield to reduce greatly; Carrying out petrolic solvent when extracting, cause oil soluble impurity to get in the product, cause saponin product fusing point low and deposit and be prone to flavescence in the process, but also can produce more colloidal substance mother liquor; Starch and cellulosic hydrolysis make that sour consumption and wastewater through organic matter content increase greatly in the production process; Cause environmental pollution, wastewater treatment difficulty to increase; Also by waste greatly, cost is high for the yellow ginger resource simultaneously, and yield is low; Because it is acid hydrolysis brings a large amount of sewage, very big simultaneously to the influence of environment.Therefore improve product quality, reduce and pollute, realize that zero release is those skilled in the art's problems anxious to be solved.

Summary of the invention

Order of the present invention provides a kind of quality better, cost is low, yield is high, pollute the little method with yellow ginger cleaner production saponin.

Technical scheme of the present invention may further comprise the steps:

1) dried yellow ginger raw material pulverizing is crossed 20 mesh sieves >=90%;

2) methyl alcohol or extraction using alcohol: add methyl alcohol or the ethanol of the 80-90% of 3～5 times of yellow ginger weight, extract each 1.5 hours three times;

3) concentrate and reclaim ethanol: 3 yellow ginger extracting solutions are concentrated reclaim methyl alcohol or ethanol to nothing alcohol flavor, liquid concentrator volume: yellow ginger weight=1: 2;

4) biologically transform with acid: liquid concentrator places temperature to be controlled in 35～50 ℃ the container, and with mikrobe ferment at constant temperature 8～20 hours, mikrobe was yeast+saccharifying enzyme, and hydrochloric acid or the sulfuric acid of adding 1-5% further carried out acid hydrolysis after fermentation was accomplished;

5) filter: the solution after will transforming filters, and gets thick saponin;

6) crystallization and recrystallization: thick saponin adopts sherwood oil to carry out crystallization and recrystallization, leaves standstill 8-12 hour, filters then, and recrystallization is till the crystallization color is pure white;

7) crystallization vacuum-drying is got finished product.

Produce saponin with method of the present invention, can output be brought up to more than 0.65% from 0.5～0.55%, compare and improve 0.1～0.15%, promptly equal raw material can be how saponin more than 20%; Improved the color and luster of saponin product in addition, removed common variety with beige to pearl, further improved product colour, make the finished product become the high-quality saponin of white non-variegation.Thereby and do not re-use a large amount of acid hydrolysiss and cause large amount of sewage discharging and cause environmental pollution etc.; The substitute is and extract the living weight that dwindles conversion significantly earlier; Realize zero release, be very easy to operation, simplified production operation and production link; Make that production is controlled easily and realized greatly, improved labor safety and protection.

Embodiment

Embodiment: get dry yellow ginger 500kg, be crushed to 20 mesh sieves >=90%, add 85% ethanol 1600L, extract each 1.5 hours three times; 3 yellow ginger extracting solutions are concentrated recovery ethanol to there not being the alcohol flavor, and promptly the liquid concentrator volume is 250L; Liquid concentrator is placed in the enamel reaction still, and in 35～50 ℃ temperature, ferment at constant temperature 8～20 hours adds 75L hydrochloric acid then with the special microorganism of yeast+saccharifying enzyme; The solution after transforming is filtered in 80 degree hydrolysis 2 hours, gets thick saponin, and thick saponin adopts sherwood oil heating for dissolving postcooling to carry out crystallization and recrystallization; Left standstill 8-12 hour, and filtered recrystallization then; Till the crystallization color is pure white, at last crystallization vacuum-drying is got finished product 12.1kg, qualified through detecting saponin.

Claims (2)

1. method with yellow ginger cleaner production saponin is characterized in that its step is following:

1) dried yellow ginger raw material pulverizing is crossed 20 mesh sieves >=90%;

2) methyl alcohol or extraction using alcohol: add methyl alcohol or the ethanol of the 80-90% of 3～5 times of yellow ginger weight, extract each 1.5 hours three times;

3) concentrate and reclaim ethanol: 3 yellow ginger extracting solutions are concentrated reclaim methyl alcohol or ethanol to nothing alcohol flavor, liquid concentrator volume: yellow ginger weight=1: 2;

4) biologically transform with acid: liquid concentrator places temperature to be controlled in 35～50 ℃ the container, and with mikrobe ferment at constant temperature 8～20 hours, hydrochloric acid or the sulfuric acid of adding 1-5% further carried out acid hydrolysis after fermentation was accomplished;

5) filter: the solution after will transforming filters, and gets thick saponin;

6) crystallization and recrystallization: thick saponin adopts sherwood oil to carry out crystallization and recrystallization, leaves standstill 8-12 hour, filters then, and recrystallization is till the crystallization color is pure white;

7) crystallization vacuum-drying is got finished product.

2. the method with yellow ginger cleaner production saponin according to claim 1 is characterized in that microorganism used therefor is yeast+saccharifying enzyme.