CN105418793A - Method for extracting pectin - Google Patents
Method for extracting pectin Download PDFInfo
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- CN105418793A CN105418793A CN201610000917.0A CN201610000917A CN105418793A CN 105418793 A CN105418793 A CN 105418793A CN 201610000917 A CN201610000917 A CN 201610000917A CN 105418793 A CN105418793 A CN 105418793A
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- pectin
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0006—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid
- C08B37/0045—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid alpha-D-Galacturonans, e.g. methyl ester of (alpha-1,4)-linked D-galacturonic acid units, i.e. pectin, or hydrolysis product of methyl ester of alpha-1,4-linked D-galacturonic acid units, i.e. pectinic acid; Derivatives thereof
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Materials Engineering (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medicinal Chemistry (AREA)
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- Organic Chemistry (AREA)
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- Polysaccharides And Polysaccharide Derivatives (AREA)
Abstract
Provided is a method for extracting pectin. The method is characterized by comprising the following steps that amylolytic enzyme is added to raw material potato residues including the pectin for hydrolysis and centrifugation, centrifugation products are collected and put in ultrasonic extracting equipment, the PH value is adjusted to be acidic, and extracting is carried out; centrifugation and microfiltration are carried out, supernate is taken, then ultrafiltration and concentration are carried out, micromolecules and pigment impurities are removed, ethyl alcohol is added to a concentrated solution, and the alcohol concentration is made to be 75%; full stirring is carried out, washing and centrifugation are carried out, a centrifugation product, namely, pectin is collected, drying is carried out, and the pectin is obtained. By means of the method, production cost can be reduced, product quality can be improved, and industrial operation is facilitated.
Description
The application is application number: 201110338104.X, the applying date: on October 31st, 2011, and denomination of invention is the divisional application of " a kind of ultrasonic extracting method of pectin ".
Technical field
The present invention relates to a kind of pectin extraction method, particularly relate to a kind of method utilizing ultrasonic continuous countercurrent extraction technology to extract pectin in potato slag.
Background technology
Potato slag is that sweet potato is extracting starch, the tankage produced when processing and fabricating bean vermicelli, vermicelli and other by-products.The potato slag that sweet potato produces after extracting starch, accounts for the 10%-14% of raw material, but after extracting, a large amount of potato slag of generation is mainly used in animal-feed or directly abandons, and major part is not developed, and not only causes waste resource, also causes contaminate environment.Research finds containing abundant pectin in potato slag, and Functionality, quality and appealing design, be good pectin source.China is sweet potato producing country maximum in the world, abundant raw material source.
Pectin is water miscible; this character is utilized to arrive so far; the method that pectin is extracted in domestic and international research from sweet potato residue, potato residues, Pericarpium Mali pumilae, Pericarpium Musae, shaddock ped etc. is more; mainly contain simple sour formulation, ion-exchange-resin process, microbial method, microwave method etc.; but be all in research level, do not accomplish scale production.And simple acid extraction method extraction pectin efficiency is lower, technology is also in constantly developing; Ion-exchange-resin process extracts pectin owing to being subject between polyvalent cation, fibrous pectic substance macromole and other polymeric mechanicalness impedes the quality affecting pectin.
The present invention takes ultrasonic method to extract, and ultrasonic method is used for the extraction of natural component, and selectivity is strong, solvent consumption is little, the operating time is short, solvent consumption target components yield is high, and can retain the natural radioactivity of natural component greatly.Along with the development of electronic technology and automatic industrial, make ultrasonic application increasingly mature, its advantage is that forming steps simplifies, simple to operate, level of automation is high.Ultrasonic extracting method meets the requirement of following food, medicine and healthcare products industry, is modern food, medicine
With healthcare products industrial expansion direction.
Summary of the invention
The technical problem to be solved in the present invention is to provide a kind of method utilizing ultrasonic continuous countercurrent extractive technique to extract pectin in potato slag.The method can reduce production cost, improves the quality of products, and is convenient to industrial operation.
In order to solve the problems of the technologies described above, it is as follows that the present invention proposes technical scheme:
Technical scheme of the present invention is:
Material potato slag containing pectin is added amylolytic enzyme be hydrolyzed, centrifugal, collect centrifugal thing and put in ultrasonic extraction device, regulate pH value to be acid, carry out supersound extraction; Centrifugal, micro-filtration, gets supernatant liquor, then ultrafiltration and concentration, and removing small molecules and pigment impurity, add ethanol, make determining alcohol be 75%, fully stir in concentrated solution, and washing is also centrifugal, collects centrifugal thing and pectin, dry, to obtain final product.
The solvent of potato slag raw materials quality 5 ~ 30 times is put, soaking at room temperature 0.5 ~ 5 hour in a container.Solvent is one or more combinations in water, methyl alcohol, ethanol, acetone, ether, methylene dichloride equal solvent.Ultrasonic frequency is 10 ~ 80KHZ, Extracting temperature 40 ~ 90 DEG C, extraction time 10 ~ 60min.Filtrate PH is that acidity refers to that PH is 1-6, and acid used is hydrochloric acid, sulfuric acid, nitric acid or mixing acid.Potato slag source is the tankage of rear generation such as sweet potato processing extraction starch making bean vermicelli, vermicelli etc.
The present invention adopts super extractive technique, owing to adopting ultrasonic organic solution circulation method to extract, has homogeneous heating, and fast, be easy to Instantaneous Control, environmental protection feature, extraction time and solvent load can be made greatly to save, and efficiency is high, output is large, reduces production cost; Supersound extraction is electrical equipment, does not need to be equipped with boiler, pollution-free, safely, belong to green engineering.
Embodiment
Embodiment
Embodiment 1
Get potato slag 100g, add amylase at 80 DEG C of hydrolysis 30min, removing remaining starch, centrifugal, collect centrifugal thing, the 1000ml that adds water makes into the suspension that solid-liquid ratio is 1: 10, ultrasonic extraction is taked to extract pectin: ultrasonic power is 15KHz, pH value is regulated to be 2.0, extract 15 minutes, centrifugal, micro-filtration (filter opening is 0.8 μm), get supernatant liquor, ultrafiltration and concentration again, removing small molecules and pigment impurity, ethanol is added in concentrated solution, determining alcohol is made to be 75%, fully stir, washing is also centrifugal, collect centrifugal thing and pectin, vacuum-drying obtains pectin dry product 8g, sulfuric acid anthrone method is checked, obtain the pectin finished product of content >=70%.
Embodiment 2
Get potato slag 100g, add amylase at 60 DEG C of hydrolysis 30min, removing remaining starch, centrifugal, collect centrifugal thing, the 500ml that adds water makes into ethanol 500ml the suspension that solid-liquid ratio is 1: 10, ultrasonic extraction is taked to extract pectin: ultrasonic power is 30KHz, pH value is regulated to be 2.0, extract 15 minutes, centrifugal, micro-filtration (filter opening is 0.8 μm), get supernatant liquor, ultrafiltration and concentration again, removing small molecules and pigment impurity, ethanol is added in concentrated solution, determining alcohol is made to be 75%, fully stir, washing is also centrifugal, collect centrifugal thing and pectin, vacuum-drying obtains pectin dry product 11g, sulfuric acid anthrone method is checked, obtain the pectin finished product of content >=70%.
Claims (1)
1. the extracting method of a pectin, it is characterized in that described method comprises the following steps: to get potato slag 100g, add amylase at 60 DEG C of hydrolysis 30min, removing remaining starch, centrifugal, collect centrifugal thing, the 500ml that adds water makes into ethanol 500ml the suspension that solid-liquid ratio is 1: 10, and described solid-liquid ratio refers to the ratio between mass unit g and volume unit ml; Ultrasonic extraction is taked to extract pectin: ultrasonic power is 30KHz, adjust ph is 2.0, extract 15 minutes, centrifugal, filter opening is 0.8 μm, get supernatant liquor, ultrafiltration and concentration again, removing small molecules and pigment impurity, add ethanol in concentrated solution, make determining alcohol be 75%, described determining alcohol refers to volumetric concentration; Fully stir, washing is also centrifugal, collects centrifugal thing and pectin, vacuum-drying obtains pectin dry product 11g, sulfuric acid anthrone method is checked, and obtains the pectin finished product of content >=70%, the tankage of described potato slag source for producing after sweet potato processing extraction starch making bean vermicelli, vermicelli.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201110338104.XA CN102399302B (en) | 2011-10-31 | 2011-10-31 | A kind of ultrasonic extracting method of pectin |
Related Parent Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201110338104.XA Division CN102399302B (en) | 2011-10-31 | 2011-10-31 | A kind of ultrasonic extracting method of pectin |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105418793A true CN105418793A (en) | 2016-03-23 |
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Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610000917.0A Pending CN105418793A (en) | 2011-10-31 | 2011-10-31 | Method for extracting pectin |
| CN201110338104.XA Expired - Fee Related CN102399302B (en) | 2011-10-31 | 2011-10-31 | A kind of ultrasonic extracting method of pectin |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201110338104.XA Expired - Fee Related CN102399302B (en) | 2011-10-31 | 2011-10-31 | A kind of ultrasonic extracting method of pectin |
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| CN (2) | CN105418793A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108641014A (en) * | 2018-05-23 | 2018-10-12 | 广东医科大学 | A kind of clausenae Lansium pectin preparation method and the clausenae Lansium pectin using this method preparation |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103304681B (en) * | 2013-06-20 | 2016-03-16 | 中国农业科学院农产品加工研究所 | A kind of method extracting pectin |
| CN104086667B (en) * | 2014-06-11 | 2017-03-08 | 安徽农业大学 | A kind of method preparing citruss skin slag pectin using ultrasonic assistant extraction |
| CN104513324A (en) * | 2014-12-17 | 2015-04-15 | 广西壮族自治区农业科学院农产品加工研究所 | Method for extracting pectin from pitaya stem |
| CN105566512B (en) * | 2015-12-14 | 2018-03-30 | 山东省果树研究所 | A kind of extracting method of persimmon fruit pectin |
| CN106519067A (en) * | 2016-11-19 | 2017-03-22 | 朱莉 | Comprehensive utilization method for sweet potato residues |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101041699A (en) * | 2006-03-22 | 2007-09-26 | 中国农业科学院农产品加工研究所 | Sweet potato pectin and production technique thereof |
| EP2014682A1 (en) * | 2006-03-17 | 2009-01-14 | Fuji Oil Company, Ltd. | Process for production of pectin, and gelling agent and gelatinous food using the same |
| CN101891840A (en) * | 2010-08-09 | 2010-11-24 | 黑龙江农垦北大荒马铃薯产业有限公司 | Method for extracting pectin by utilizing potato scraps |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP3511952B2 (en) * | 1999-01-19 | 2004-03-29 | 不二製油株式会社 | Pectin, its production method, acidic protein food using the same, and its production method |
| CN101942036A (en) * | 2009-07-08 | 2011-01-12 | 李浩贤 | Method for extracting pectin from pineapple peel assisted by ultrasonic wave |
| CN101805416B (en) * | 2010-03-16 | 2011-07-20 | 上海交通大学 | Preparation method of canna edulis ker pectin |
-
2011
- 2011-10-31 CN CN201610000917.0A patent/CN105418793A/en active Pending
- 2011-10-31 CN CN201110338104.XA patent/CN102399302B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2014682A1 (en) * | 2006-03-17 | 2009-01-14 | Fuji Oil Company, Ltd. | Process for production of pectin, and gelling agent and gelatinous food using the same |
| CN101041699A (en) * | 2006-03-22 | 2007-09-26 | 中国农业科学院农产品加工研究所 | Sweet potato pectin and production technique thereof |
| CN101891840A (en) * | 2010-08-09 | 2010-11-24 | 黑龙江农垦北大荒马铃薯产业有限公司 | Method for extracting pectin by utilizing potato scraps |
Non-Patent Citations (4)
| Title |
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| 李全宏主编: "《农副产品综合利用（第1版）》", 30 September 2009, 中国农业大学出版社 * |
| 李新华等: "《粮油加工工艺学（第1版）》", 30 September 2011, 郑州大学出版社 * |
| 杨希娟等: "马铃薯渣中提取果胶的工艺优化及产品成分分析", 《食品科学》 * |
| 王俊儒等: "《天然产物提取分离与鉴定技术（第1版）》", 31 May 2006, 西北农林科技大学出版社 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108641014A (en) * | 2018-05-23 | 2018-10-12 | 广东医科大学 | A kind of clausenae Lansium pectin preparation method and the clausenae Lansium pectin using this method preparation |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102399302A (en) | 2012-04-04 |
| CN102399302B (en) | 2016-06-01 |
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Application publication date: 20160323 |