CN1273795A - Process for preparing spirulina biliprtein beverage - Google Patents
Process for preparing spirulina biliprtein beverage Download PDFInfo
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- CN1273795A CN1273795A CN00107763A CN00107763A CN1273795A CN 1273795 A CN1273795 A CN 1273795A CN 00107763 A CN00107763 A CN 00107763A CN 00107763 A CN00107763 A CN 00107763A CN 1273795 A CN1273795 A CN 1273795A
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- phycobniliprotein
- spirulina
- beverage
- solubilizer
- dry powder
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Abstract
A spirulina biliprotein beverage is prepared from mashed fresh spirulina or powdered spirulina and calcium chloride as solubilizing agent through proportional mixing, centrifugas separation, dialysis of blue supernatant to obtain dried biliprotein powder and mixing it with liquid. Its advantages include green health-care beverage, no odour, good enjoyment to drink it, and easy digestion and absorption.
Description
The present invention relates to a kind of preparation method of spirulina biliprtein beverage, belong to biological technical field.
Spirulina is a kind of many cells filamentous cyanobacteria, includes a kind of other biological unexistent special chromoprotein-phycobniliprotein, and its content accounts for the 10-24.8% of frond dry weight.Phycobniliprotein can improve the human immunologic function, strengthen hematopoiesis function, also can be used for the supplemental treatment of diseases such as tumour, anaemia.Therefore, in the world with domestic some biotech companies (as the EARTHRISE company of the U.S., the many companies and the producer of China) all utilize cell cultivation and cell engineering to produce spirulina, at u.s.a. and japan the production base of spirulina is arranged all, spirulina is always in occupation of certain market share on the nutrient and healthcare products market of the U.S. and Japan.The spirulina production technology is by importing China abroad in recent years, and domestic various places have been set up tens tame spirulina factory and company, production and selling spiral algae powder and capsules at home.Because the sale and the propaganda of spirulina, people understand gradually, admit and believe the alimentary health-care function of spirulina.
Domestic production technology that a kind of spiral algae powder and capsule are only arranged and products thereof because the spirulina production technology is difficult to by simple monopoly, causes manufacturer numerous, Market competition thus but up to now.Simultaneously, because the extraction process complexity of spirulina biliprtein, the cost of extraction is higher relatively, and the stability of phycobniliprotein solution is very low, and the domestic and international market does not also have this product of spirulina biliprtein beverage or commodity at present.
The objective of the invention is to propose a kind of preparation method of spirulina biliprtein beverage, utilize that the spirulina biliprtein beverage of this method preparation has that alimentary health-care function is strong, good stability, can long preservation and the preparation manipulation process easy, characteristics such as cost is low can be filled up the blank of this kind product on the market.
The preparation method of the spirulina biliprtein beverage that the present invention proposes comprises following each step:
1, be raw material with fresh algae mud of spirulina or algae powder, this raw material generally can be bought from domestic how tame spirulina company, comprises that with plurality of inorganic salt sodium chloride, sodium acetate, calcium chloride etc. are mixed with solubilizer by 0.01-0.5M concentration.(ml: g) mix, lucifuge was handled 2-3 days under 2-6 ℃ of condition, stirred 6-8 time between every day is artificial by 1: 1～10: 1 with solubilizer and fresh algae mud.Perhaps (ml: g) mix, lucifuge was handled 2-3 days under 2-6 ℃ of condition, stirred 6-8 time between every day is artificial by 50: 1～200: 1 with solubilizer and algae powder.
2, aforesaid liquid being removed cell fragment with centrifugal or isolated by filtration method, is that phycobniliprotein solution is done dialysis treatment to blue supernatant, removes the inorganic salts in the solubilizer.
3, the phycobniliprotein solution low temperature drying of solubilizer will be removed, phycobniliprotein dry powder can be obtained.
4, promptly drink instant, be convenient to transportation and preservation: need phycobniliprotein dry powder be enclosed in a container or the pouch with the ratio of 2-10mg phycobniliprotein dry powder in every 500ml beverage, the process of drinking the front opening beverage bottle cap just in time is discharged into phycobniliprotein dry powder dissolving in time in the liquid in the bottle, at this moment, water white beverage presents the distinctive blue-fluorescence of phycobniliprotein solution immediately in the former bottle.
This invention has following advantage:
1, the phycobniliprotein extractive technique of low temperature self-dissolving of Jian Huaing and solubilising: adopt fresh algae mud to replace spirulina cells dry powder, reduced because of the required cost of spray-drying on the one hand, can increase the extraction efficiency and the productive rate of phycobniliprotein on the other hand; The method of low temperature self-dissolving is easier than traditional various method of cell disruption and chromatography method extraction separation phycobniliprotein, does not also need special instrument and equipment and operating process; The electrolyte (the needed nutritive salt of a kind of growth of spirulina platensis) that adds low concentration when self-dissolving can improve the stripping quantity of phycobniliprotein to greatest extent.
2, promptly drink instant technology: phycobniliprotein dry powder is encapsulated in the bottle cap, and the process of drinking the front opening bottle cap just in time is discharged into phycobniliprotein dry powder dissolving in time in the liquid in the bottle, has solved the problem that phycobniliprotein liquid is difficult to preserve.Be that phycobniliprotein is under liquid condition, the blue-fluorescence that shows phycobniliprotein will fade because of the decomposition of pigment after seeing light in tens hours, instantly can guarantee and show the blue-fluorescence of beverage and the existence of its function of phycobniliprotein composition when drinking and promptly drink.
3, the green health care food beverage of pure natural, active material and active ingredient in the spirulina cells are extracted, and its effect is stronger.
4, do not have the fishy smell of spiral algae powder, capsule and tablet, mouthfeel is better, and easier quilt digests and absorbs.
Introduce embodiments of the invention below:
Embodiment one
100.0 gram algae mud adds 0.1M calcium chloride solution (solubilizer) 200ml, lucifuge was handled 3 days under 4 ℃ of conditions, stirred 6 times between every day is artificial.Aforesaid liquid is joined in the centrifuge tube, weigh balance in the balance after, (16,000rpm 15min), makes cell fragment precipitation to carry out low-temperature centrifugation on JZ-MC high speed freezing centrifuge (U.S. BECKMAN company).The blue supernatant of getting in the centrifuge tube is a phycobniliprotein solution, joins in the commercially available bag filter, and bag filter is put into 1000ml distilled water dialysis 24 hours, wherein changes water once in 12 hours.Phycobniliprotein solution in the bag filter is joined in the culture dish, put into the freeze drier freeze drying, can obtain phycobniliprotein dry powder.Phycobniliprotein yield: 8.90%.Phycobniliprotein dry powder is stored in 4 ℃ of refrigerators, through the characteristic spectrum analytical proof, is stored in and still can keeps its original quality in 4 ℃ of refrigerators after 6 months.Phycobniliprotein dry powder 10mg is enclosed on the bottle cap, and the process of drinking the front opening bottle cap just in time is discharged into phycobniliprotein dry powder dissolving in time in the liquid in the bottle, and at this moment, water white beverage presents the distinctive blue-fluorescence of phycobniliprotein solution immediately in the former bottle.
Embodiment two
200.0 gram algae mud adds 0.2M calcium chloride solution (solubilizer) 400ml, lucifuge was handled 3 days under 4 ℃ of conditions, stirred 8 times between every day is artificial.Aforesaid liquid is joined in the centrifuge tube, weigh balance in the balance after, (15,000rpm 25min), makes cell fragment precipitation to carry out low-temperature centrifugation on JZ-MC high speed freezing centrifuge (U.S. BECKMAN company).The blue supernatant of getting in the centrifuge tube is a phycobniliprotein solution, joins in the commercially available bag filter, and bag filter is put into 1000ml distilled water dialysis 24 hours, wherein changes water once in 8 hours.Phycobniliprotein solution in the bag filter is joined in the culture dish, put into the freeze drier freeze drying, can obtain phycobniliprotein dry powder, phycobniliprotein yield: 11.99.Phycobniliprotein dry powder is stored in 4 ℃ of refrigerators, through the characteristic spectrum analytical proof, is stored in and still can keeps its original quality in 4 ℃ of refrigerators after 6 months.Phycobniliprotein dry powder 2mg is enclosed in the paper bag, drink the front opening bottle cap, the phycobniliprotein dry powder of paper bag is dissolved in the liquid in the bottle; At this moment, water white beverage presents the distinctive blue-fluorescence of phycobniliprotein solution immediately in the former bottle.
Embodiment three
5.0 gram algae powder adds 0.4M calcium chloride solution (solubilizer) 500ml, lucifuge was handled 2 days under 4 ℃ of conditions, stirred 7 times between every day is artificial.Aforesaid liquid is joined in the centrifuge tube, weigh balance in the balance after, (16,000rpm 15min), makes cell fragment precipitation to carry out low-temperature centrifugation on JZ-MC high speed freezing centrifuge (U.S. BECKMAN company).Cell precipitation is extracted phycobniliprotein 2 times repeatedly by above-mentioned steps again, and the blue supernatant of getting in the centrifuge tube is a phycobniliprotein solution, joins in the commercially available bag filter, and bag filter is put into 1000ml distilled water dialysis 24 hours, wherein changes water once in 12 hours.Phycobniliprotein solution in the bag filter is joined in the culture dish, put into the freeze drier freeze drying, can obtain phycobniliprotein dry powder, phycobniliprotein yield: 13.20%.Phycobniliprotein dry powder is stored in 4 ℃ of refrigerators, through the characteristic spectrum analytical proof, is stored in and still can keeps its original quality in 4 ℃ of refrigerators after 6 months.Phycobniliprotein dry powder 5mg is enclosed in the bottle cap, and the process of drinking the front opening bottle cap just in time is discharged into phycobniliprotein dry powder dissolving in time in the liquid in the bottle, and at this moment, water white beverage presents the distinctive blue-fluorescence of phycobniliprotein solution immediately in the former bottle.
Claims (1)
1, a kind of preparation method of spirulina biliprtein beverage is characterized in that, this method comprises following each step:
(1) be raw material with fresh algae mud of spirulina or algae powder, the calcium chloride that with concentration is 0.01-0.5M is solubilizer, with solubilizer and fresh algae mud by 1～10ml: 1g mixes, lucifuge was handled 2-3 days under 2-6 ℃ of condition, stir 6-8 time between every day is artificial, or with solubilizer and algae powder by 50～200ml: 1g mixes, and lucifuge was handled 2-3 days under 2-6 ℃ of condition, stirs 6-8 time between every day is artificial;
(2) aforesaid liquid being removed cell fragment with centrifugal or isolated by filtration method, is that phycobniliprotein solution is done dialysis treatment to blue supernatant, removes the inorganic salts in the solubilizer;
(3) will remove the phycobniliprotein solution low temperature drying of solubilizer, promptly obtain phycobniliprotein dry powder;
(4) be that the ratio of 2-10mg phycobniliprotein dry powder is enclosed phycobniliprotein dry powder in one container or the pouch in every 500ml beverage, drink the container that front opening is equipped with beverage, phycobniliprotein dry powder is discharged into dissolving in time in the bottle, is the spirulina biliprtein beverage of the present invention's preparation.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN00107763A CN1095347C (en) | 2000-05-26 | 2000-05-26 | Process for preparing spirulina biliprtein beverage |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN00107763A CN1095347C (en) | 2000-05-26 | 2000-05-26 | Process for preparing spirulina biliprtein beverage |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1273795A true CN1273795A (en) | 2000-11-22 |
| CN1095347C CN1095347C (en) | 2002-12-04 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN00107763A Expired - Fee Related CN1095347C (en) | 2000-05-26 | 2000-05-26 | Process for preparing spirulina biliprtein beverage |
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| Country | Link |
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| CN (1) | CN1095347C (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104687101A (en) * | 2015-02-09 | 2015-06-10 | 福州大学 | Food rich in laver phycobiliprotein |
| CN109734264A (en) * | 2018-11-20 | 2019-05-10 | 江南大学 | A method of promoting the release of bloom blue algae content |
| FR3106726A1 (en) | 2020-02-04 | 2021-08-06 | Jacques Degro0Te | Preparation of an extract rich in phycobiliproteins |
-
2000
- 2000-05-26 CN CN00107763A patent/CN1095347C/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104687101A (en) * | 2015-02-09 | 2015-06-10 | 福州大学 | Food rich in laver phycobiliprotein |
| CN109734264A (en) * | 2018-11-20 | 2019-05-10 | 江南大学 | A method of promoting the release of bloom blue algae content |
| FR3106726A1 (en) | 2020-02-04 | 2021-08-06 | Jacques Degro0Te | Preparation of an extract rich in phycobiliproteins |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1095347C (en) | 2002-12-04 |
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