CN1095347C - Process for preparing spirulina biliprtein beverage - Google Patents
Process for preparing spirulina biliprtein beverage Download PDFInfo
- Publication number
- CN1095347C CN1095347C CN00107763A CN00107763A CN1095347C CN 1095347 C CN1095347 C CN 1095347C CN 00107763 A CN00107763 A CN 00107763A CN 00107763 A CN00107763 A CN 00107763A CN 1095347 C CN1095347 C CN 1095347C
- Authority
- CN
- China
- Prior art keywords
- phycobniliprotein
- beverage
- spirulina
- solubilizer
- powder
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 235000016425 Arthrospira platensis Nutrition 0.000 title claims abstract description 31
- 240000002900 Arthrospira platensis Species 0.000 title claims abstract description 31
- 229940082787 spirulina Drugs 0.000 title claims abstract description 30
- 235000013361 beverage Nutrition 0.000 title claims abstract description 24
- 238000004519 manufacturing process Methods 0.000 title description 6
- 239000000843 powder Substances 0.000 claims abstract description 35
- 239000002904 solvent Substances 0.000 claims abstract description 15
- 238000000034 method Methods 0.000 claims abstract description 14
- 239000007788 liquid Substances 0.000 claims abstract description 13
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 claims abstract description 6
- 239000002994 raw material Substances 0.000 claims abstract description 4
- 239000001110 calcium chloride Substances 0.000 claims abstract description 3
- 229910001628 calcium chloride Inorganic materials 0.000 claims abstract description 3
- 238000001914 filtration Methods 0.000 claims abstract description 3
- 241000195493 Cryptophyta Species 0.000 claims description 15
- 238000002360 preparation method Methods 0.000 claims description 8
- 230000003203 everyday effect Effects 0.000 claims description 7
- 238000000502 dialysis Methods 0.000 claims description 5
- 239000012634 fragment Substances 0.000 claims description 5
- 239000006228 supernatant Substances 0.000 claims description 5
- 150000003839 salts Chemical class 0.000 claims description 3
- 238000001035 drying Methods 0.000 claims description 2
- 238000003756 stirring Methods 0.000 claims 2
- 239000002775 capsule Substances 0.000 abstract description 4
- 238000000926 separation method Methods 0.000 abstract description 3
- 239000003826 tablet Substances 0.000 abstract description 2
- 108060006184 phycobiliprotein Proteins 0.000 abstract 5
- 239000000243 solution Substances 0.000 abstract 1
- 239000010414 supernatant solution Substances 0.000 abstract 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 10
- 230000035622 drinking Effects 0.000 description 5
- 238000000605 extraction Methods 0.000 description 4
- 238000001556 precipitation Methods 0.000 description 4
- 239000000047 product Substances 0.000 description 4
- 238000005119 centrifugation Methods 0.000 description 3
- 239000012153 distilled water Substances 0.000 description 3
- 238000004108 freeze drying Methods 0.000 description 3
- 238000007710 freezing Methods 0.000 description 3
- 230000008014 freezing Effects 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 238000001228 spectrum Methods 0.000 description 3
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 238000004321 preservation Methods 0.000 description 2
- 241000192700 Cyanobacteria Species 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 description 1
- 239000004480 active ingredient Substances 0.000 description 1
- 239000011149 active material Substances 0.000 description 1
- 208000007502 anemia Diseases 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000003792 electrolyte Substances 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 230000011132 hemopoiesis Effects 0.000 description 1
- 230000036737 immune function Effects 0.000 description 1
- 229910017053 inorganic salt Inorganic materials 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 239000000049 pigment Substances 0.000 description 1
- 239000001632 sodium acetate Substances 0.000 description 1
- 235000017281 sodium acetate Nutrition 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 238000001694 spray drying Methods 0.000 description 1
- 230000000153 supplemental effect Effects 0.000 description 1
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Non-Alcoholic Beverages (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The present invention relates to a method for preparing a spirulina phycobiliprotein beverage, which comprises the following steps: firstly, fresh spirulina mud or spirulina powder is used as a raw material, and calcium chloride is used as a solubilizer, the solubilizer and the fresh spirulina mud are mixed proportionally, cell debris is removed by a centrifugal separation method of a filtering separation method, and a blue supernatant solution (phycobiliprotein solution) is dialyzed to obtain dry phycobiliprotein powder; the dry phycobiliprotein powder and liquid are mixed to obtain the spirulina phycobiliprotein beverage which is prepared by the method of the present invention. The beverage which is prepared by the present invention belongs to a pure natural green health-care beverage, and does not have the stink of spirulina powder, capsules and tablets; the beverage has good taste, and is easy to digest and absorb.
Description
The present invention relates to a kind of preparation method of spirulina biliprtein beverage, belong to biological technical field.
Spirulina is a kind of many cells filamentous cyanobacteria, includes a kind of other biological unexistent special chromoprotein-phycobniliprotein, and its content accounts for the 10-24.8% of frond dry weight.Phycobniliprotein can improve the human immunologic function, strengthen hematopoiesis function, also can be used for the supplemental treatment of diseases such as tumour, anaemia.Therefore, in the world with domestic some biotech companies (as the EARTHRISE company of the U.S., the many companies and the producer of China) all utilize cell cultivation and cell engineering to produce spirulina, at u.s.a. and japan the production base of spirulina is arranged all, spirulina is always in occupation of certain market share on the nutrient and healthcare products market of the U.S. and Japan.The spirulina production technology is by importing China abroad in recent years, and domestic various places have been set up tens tame spirulina factory and company, production and selling spiral algae powder and capsules at home.Because the sale and the propaganda of spirulina, people understand gradually, admit and believe the alimentary health-care function of spirulina.
Domestic production technology that a kind of spiral algae powder and capsule are only arranged and products thereof because the spirulina production technology is difficult to by simple monopoly, causes manufacturer numerous, Market competition thus but up to now.Simultaneously, because the extraction process complexity of spirulina biliprtein, the cost of extraction is higher relatively, and the stability of phycobniliprotein solution is very low, and the domestic and international market does not also have this product of spirulina biliprtein beverage or commodity at present.
The objective of the invention is to propose a kind of preparation method of spirulina biliprtein beverage, utilize that the spirulina biliprtein beverage of this method preparation has that alimentary health-care function is strong, good stability, can long preservation and the preparation manipulation process easy, characteristics such as cost is low can be filled up the blank of this kind product on the market.
The preparation method of the spirulina biliprtein beverage that the present invention proposes comprises following each step:
1, be raw material with fresh algae mud of spirulina or algae powder, this raw material generally can be bought from domestic how tame spirulina company, comprises that with plurality of inorganic salt sodium chloride, sodium acetate, calcium chloride etc. are mixed with solubilizer by 0.01-0.5M concentration.(ml: g) mix, lucifuge was handled 2-3 days under 2-6 ℃ of condition, stirred 6-8 time between every day is artificial by 1: 1~10: 1 with solubilizer and fresh algae mud.Perhaps (ml: g) mix, lucifuge was handled 2-3 days under 2-6 ℃ of condition, stirred 6-8 time between every day is artificial by 50: 1~200: 1 with solubilizer and algae powder.
2, aforesaid liquid being removed cell fragment with centrifugal or isolated by filtration method, is that phycobniliprotein solution is done dialysis treatment to blue supernatant, removes the inorganic salts in the solubilizer.
3, the phycobniliprotein solution low temperature drying of solubilizer will be removed, phycobniliprotein dry powder can be obtained.
4, promptly drink instant, be convenient to transportation and preservation: need phycobniliprotein dry powder be enclosed in a container or the pouch with the ratio of 2-10mg phycobniliprotein dry powder in every 500ml beverage, the process of drinking the front opening beverage bottle cap just in time is discharged into phycobniliprotein dry powder dissolving in time in the liquid in the bottle, at this moment, water white beverage presents the distinctive blue-fluorescence of phycobniliprotein solution immediately in the former bottle.
This invention has following advantage:
1, the phycobniliprotein extractive technique of low temperature self-dissolving of Jian Huaing and solubilising: adopt fresh algae mud to replace spirulina cells dry powder, reduced because of the required cost of spray-drying on the one hand, can increase the extraction efficiency and the productive rate of phycobniliprotein on the other hand; The method of low temperature self-dissolving is easier than traditional various method of cell disruption and chromatography method extraction separation phycobniliprotein, does not also need special instrument and equipment and operating process; The electrolyte (the needed nutritive salt of a kind of growth of spirulina platensis) that adds low concentration when self-dissolving can improve the stripping quantity of phycobniliprotein to greatest extent.
2, promptly drink instant technology: phycobniliprotein dry powder is encapsulated in the bottle cap, and the process of drinking the front opening bottle cap just in time is discharged into phycobniliprotein dry powder dissolving in time in the liquid in the bottle, has solved the problem that phycobniliprotein liquid is difficult to preserve.Be that phycobniliprotein is under liquid condition, the blue-fluorescence that shows phycobniliprotein will fade because of the decomposition of pigment after seeing light in tens hours, instantly can guarantee and show the blue-fluorescence of beverage and the existence of its function of phycobniliprotein composition when drinking and promptly drink.
3, the green health care food beverage of pure natural, active material and active ingredient in the spirulina cells are extracted, and its effect is stronger.
4, do not have the fishy smell of spiral algae powder, capsule and tablet, mouthfeel is better, and easier quilt digests and absorbs.
Introduce embodiments of the invention below:
Embodiment one
100.0 gram algae mud adds 0.1M calcium chloride solution (solubilizer) 200ml, lucifuge was handled 3 days under 4 ℃ of conditions, stirred 6 times between every day is artificial.Aforesaid liquid is joined in the centrifuge tube, weigh balance in the balance after, (16,000rpm 15min), makes cell fragment precipitation to carry out low-temperature centrifugation on JZ-MC high speed freezing centrifuge (U.S. BECKMAN company).The blue supernatant of getting in the centrifuge tube is a phycobniliprotein solution, joins in the commercially available bag filter, and bag filter is put into 1000ml distilled water dialysis 24 hours, wherein changes water once in 12 hours.Phycobniliprotein solution in the bag filter is joined in the culture dish, put into the freeze drier freeze drying, can obtain phycobniliprotein dry powder.Phycobniliprotein yield: 8.90%.Phycobniliprotein dry powder is stored in 4 ℃ of refrigerators, through the characteristic spectrum analytical proof, is stored in and still can keeps its original quality in 4 ℃ of refrigerators after 6 months.Phycobniliprotein dry powder 10mg is enclosed on the bottle cap, and the process of drinking the front opening bottle cap just in time is discharged into phycobniliprotein dry powder dissolving in time in the liquid in the bottle, and at this moment, water white beverage presents the distinctive blue-fluorescence of phycobniliprotein solution immediately in the former bottle.
Embodiment two
200.0 gram algae mud adds 0.2M calcium chloride solution (solubilizer) 400ml, lucifuge was handled 3 days under 4 ℃ of conditions, stirred 8 times between every day is artificial.Aforesaid liquid is joined in the centrifuge tube, weigh balance in the balance after, (15,000rpm 25min), makes cell fragment precipitation to carry out low-temperature centrifugation on JZ-MC high speed freezing centrifuge (U.S. BECKMAN company).The blue supernatant of getting in the centrifuge tube is a phycobniliprotein solution, joins in the commercially available bag filter, and bag filter is put into 1000ml distilled water dialysis 24 hours, wherein changes water once in 8 hours.Phycobniliprotein solution in the bag filter is joined in the culture dish, put into the freeze drier freeze drying, can obtain phycobniliprotein dry powder, phycobniliprotein yield: 11.99.Phycobniliprotein dry powder is stored in 4 ℃ of refrigerators, through the characteristic spectrum analytical proof, is stored in and still can keeps its original quality in 4 ℃ of refrigerators after 6 months.Phycobniliprotein dry powder 2mg is enclosed in the paper bag, drink the front opening bottle cap, the phycobniliprotein dry powder of paper bag is dissolved in the liquid in the bottle; At this moment, water white beverage presents the distinctive blue-fluorescence of phycobniliprotein solution immediately in the former bottle.
Embodiment three
5.0 gram algae powder adds 0.4M calcium chloride solution (solubilizer) 500ml, lucifuge was handled 2 days under 4 ℃ of conditions, stirred 7 times between every day is artificial.Aforesaid liquid is joined in the centrifuge tube, weigh balance in the balance after, (16,000rpm 15min), makes cell fragment precipitation to carry out low-temperature centrifugation on JZ-MC high speed freezing centrifuge (U.S. BECKMAN company).Cell precipitation is extracted phycobniliprotein 2 times repeatedly by above-mentioned steps again, and the blue supernatant of getting in the centrifuge tube is a phycobniliprotein solution, joins in the commercially available bag filter, and bag filter is put into 1000ml distilled water dialysis 24 hours, wherein changes water once in 12 hours.Phycobniliprotein solution in the bag filter is joined in the culture dish, put into the freeze drier freeze drying, can obtain phycobniliprotein dry powder, phycobniliprotein yield: 13.20%.Phycobniliprotein dry powder is stored in 4 ℃ of refrigerators, through the characteristic spectrum analytical proof, is stored in and still can keeps its original quality in 4 ℃ of refrigerators after 6 months.Phycobniliprotein dry powder 5mg is enclosed in the bottle cap, and the process of drinking the front opening bottle cap just in time is discharged into phycobniliprotein dry powder dissolving in time in the liquid in the bottle, and at this moment, water white beverage presents the distinctive blue-fluorescence of phycobniliprotein solution immediately in the former bottle.
Claims (1)
1, a kind of preparation method of spirulina biliprtein beverage is characterized in that, this method comprises following each step:
(1) be raw material with fresh algae mud of spirulina or algae powder, the calcium chloride that with concentration is 0.01-0.5M is solubilizer, with solubilizer and fresh algae mud by 1~10ml: 1g mixes, lucifuge was handled 2-3 days under 2-6 ℃ of condition, stir 6-8 time between every day is artificial, or with solubilizer and algae powder by 50~200ml: 1g mixes, and lucifuge was handled 2-3 days under 2-6 ℃ of condition, stirs 6-8 time between every day is artificial;
(2) aforesaid liquid being removed cell fragment with centrifugal or isolated by filtration method, is that phycobniliprotein solution is done dialysis treatment to blue supernatant, removes the inorganic salts in the solubilizer;
(3) will remove the phycobniliprotein solution low temperature drying of solubilizer, promptly obtain phycobniliprotein dry powder;
(4) be that the ratio of 2-10mg phycobniliprotein dry powder is enclosed phycobniliprotein dry powder in one container or the pouch in every 500ml beverage, drink the container that front opening is equipped with beverage, phycobniliprotein dry powder is discharged into dissolving in time in the bottle, is the spirulina biliprtein beverage of the present invention's preparation.
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CN00107763A CN1095347C (en) | 2000-05-26 | 2000-05-26 | Process for preparing spirulina biliprtein beverage |
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CN00107763A CN1095347C (en) | 2000-05-26 | 2000-05-26 | Process for preparing spirulina biliprtein beverage |
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CN1273795A CN1273795A (en) | 2000-11-22 |
CN1095347C true CN1095347C (en) | 2002-12-04 |
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CN00107763A Expired - Fee Related CN1095347C (en) | 2000-05-26 | 2000-05-26 | Process for preparing spirulina biliprtein beverage |
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Families Citing this family (3)
Publication number | Priority date | Publication date | Assignee | Title |
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CN104687101A (en) * | 2015-02-09 | 2015-06-10 | 福州大学 | Food rich in laver phycobiliprotein |
CN109734264A (en) * | 2018-11-20 | 2019-05-10 | 江南大学 | A method of promoting the release of bloom blue algae content |
FR3106726B1 (en) | 2020-02-04 | 2022-02-18 | Degro0Te Jacques | Preparation of an extract rich in phycobiliproteins |
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