CN109734264A - A method of promoting the release of bloom blue algae content - Google Patents
A method of promoting the release of bloom blue algae content Download PDFInfo
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- CN109734264A CN109734264A CN201811383135.5A CN201811383135A CN109734264A CN 109734264 A CN109734264 A CN 109734264A CN 201811383135 A CN201811383135 A CN 201811383135A CN 109734264 A CN109734264 A CN 109734264A
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Abstract
The invention discloses a kind of methods of promotion bloom blue algae content release, it is characterised in that: cyanobacteria mud promotes the organic substances such as cyanobacteria intracellular protein, polysaccharide further to be discharged by constant-temperature incubation and self-dissolving broken wall treatment, and by adding surfactant.Technical performance index of the invention its organic matter release result of extraction compared with similar technique is more superior.Large batch of salvaging cyanobacteria can be handled, be convenient for subsequent resource utilization after the dissolution to water phase of frustule broken wall organic matter.
Description
Technical field
The present invention relates to field of environment engineering technology, in particular to a kind of promotion bloom blue algae content release
Method is a kind of preconditioning technique that blue algae resource utilizes.
Background technique
Cyanobacteria is that one kind is rich in the nutriments such as protein, can be carried out photosynthetic unicellular prokaryotic.Work as water body
When middle nitrogen and phosphorus element is more than normal value, cyanobacteria mass propagation is broken out extensively in freshwater lake, forms wawter bloom.A large amount of cyanobacteria
It is accumulated in shore bank band, and then it is smelly to rot, and affects lake surface landscape.The respiration of Cells of Blue-green Algae and cyanobacteria, which rot to decompose, to disappear
A large amount of oxygen are consumed, decline oxygen content of water sharply, cause the death of the biology such as fish in water body, shrimp.And Cells of Blue-green Algae rots
The algae toxin released has extensive bio-toxicity, or even by skin contact, food chain etc. by way of endangering human health.Mesh
The preceding maximally efficient measure for inhibiting wawter bloom to spread unchecked is the algae organic matter content that the stretch of coastal water is reduced by salvaging, and slows down water quality
Deteriorate, improves water body environment.
In blue algae bloom period, only salvaging amount salvages the cyanobacteria come due to aqueous up to upper kiloton to Taihu Lake basin daily
Rate is high, brings very big difficulty to transport, storage and processing.Due to the higher unsuitable burning disposal of moisture content；Directly carry out
Landfill can also occupy the land resource of a large amount of preciousnesses；Cyanobacteria is innoxious at present, utilization approaches are extremely short of.The disposition of algal gel disappears
The ability of receiving is difficult to meet the needs of lake body cyanobacteria salvages ability, and the gap between Scavenging activity and wawter bloom accumulation is huge.Currently, opening
High value cyanobacteria biomass as resources technology is issued, is to solve one of the important channel that cyanobacterial bloom is administered.
Since the nutriment of Cells of Blue-green Algae is wrapped up by the double-layer cell wall that peptide glycan and cellulose are constituted, in order to sufficiently sharp
With cyanobacteria nutritional resource abundant intracellular, it is necessary first to improve Cells of Blue-green Algae broken wall efficiency.The Cells of Blue-green Algae broken wall of high-efficiency and economic
Frustule content dissolution efficiency can be improved in technology, promotes the yield of cyanobacteria high added value product, gives cyanobacteria biologic recycling
Utilize the technical support that offer is strong.
A variety of cyanobacteria wall breaking technologies are disclosed at present, and one is ultrasonication technology, makes cyanobacteria content using void effect
Object release, but this method treating capacity is small, it can not scale application；Secondly adding acid or alkali into cyanobacteria for acid, basic hydrolysis technology
Equal chemical agents, crack Cells of Blue-green Algae wall, but the reagent use cost of this method is high, and easily causes secondary pollution；Thirdly being
Cyanobacteria multigelation is made Cells of Blue-green Algae broken wall, but this method low efficiency using ice-crystal growth, energy consumption is high by multigelation technology.
Therefore, a kind of method for effectively facilitating the release of bloom blue algae content is studied, utilizing to blue algae resource has weight
Want meaning.
Summary of the invention
The technical problem to be solved by the invention is to provide a kind of methods of promotion bloom blue algae content release, to solve
Low efficiency of the existing technology, at high cost, energy consumption format high throughput is small and the problem of easily causing secondary pollution.
In order to solve the above-mentioned technical problem, The technical solution adopted by the invention is as follows:
Cyanobacteria is subjected to medium temperature self-dissolving processing, makes Cells of Blue-green Algae broken wall, intracellular organic matter dissolves out, and by adding surface-active
Agent makes organic matter intracellular obtain more efficient release.
Further, the cyanobacteria is the fresh cyanobacteria mud salvaged in water body, and cyanobacteria mud separates lake water by air-floating apparatus
With cyanobacteria, the moisture content of algae water is reduced, after concentration, cyanobacteria moisture content is 93%~95%.The temperature range of the medium temperature is 15-
65 DEG C, preferably 45~55 DEG C.The self-dissolving is the self-dissolving concept that this field is understood.
Further, autolysis process includes: to add surfactant in the cyanobacteria after concentration, is warming up to 45~55 DEG C,
Dissolve 4~8h.
Further, the applicable pH value range of the method is 6~8, and algal biomass can be direct without adjusting pH value
It uses.
Further, the surfactant includes dodecyl sodium sulfate, neopelex, cetyl
Trimethylammonium bromide, Tween-80 and cetyl azochlorosulfonate propyl lycine it is one or more.
Further, the dosage of surfactant be cyanobacteria in dry biomass 0.1-5%, preferably 0.5%~
2%.
Further, it is described into cyanobacteria add surfactant method be, in whipping process into cyanobacteria uniformly
Spray plus surfactant solution.
Compared with the prior art, the advantages of the present invention are as follows:
(1) this technology can carry out air-flotation process to large batch of salvaging cyanobacteria, with reaching the mesh for holding decrement.
(2) according to there is the characteristics of airbag structure in Cells of Blue-green Algae, medium temperature is selected to heat self-dissolving, is conducive to the broken of cell wall
It splits, and equipment is simple, easy to operate, low energy consumption.
(3) after Cells of Blue-green Algae broken wall, the protein for being adhered to cell wall and carbon water can be promoted using surfactant
Compound release and dissolution, dissolved rate of protein increase to 43.04% from being not added with the 34.32% of surfactant；Carbon aquation
The release of object is closed also from being not added with the 38.94% of surfactant, increases to 45.95%.Content dissolution time also from for 24 hours,
It is reduced to 4~8h, while improving treatment effeciency, is greatly saved the reaction time, reduces energy consumption and cost.
Specific embodiment
Following embodiment technology contents used to explain the present invention, but be not construed as limiting the invention.In embodiment
It is raw materials used to pass through commercially available acquisition.Fresh cyanobacteria mud is derived from Lake Taihu area in embodiment.
Embodiment 1
The fresh cyanobacteria mud of 1.0L is taken, the cyanobacteria that pH value is 6, moisture content is 95% is deployed into.Water bath with thermostatic control adds at 50 DEG C
Heat, spray plus the nonionic surfactant Tween-80 solution 5ml of 0.1g/ml concentration are stirred evenly, i.e. surface-active at this time
Agent Tween-80 dosage is 1% of amount of dry matter in cyanobacteria solution.Speed of agitator is 80rpm, reaction time 8h.Reaction
After cyanobacteria solution is put into a centrifuge to be centrifugated 10min under 7000r/min revolving speed, obtain supernatant.By supernatant
Liquid is 0.45 μm of cellulose mixture microfiltration membranes, the content of soluble protein and soluble-carbohydrate in filtrate by aperture
As add the inclusion content that surfactant Polysorbate-cyanobacteria self-dissolving after 80s releases.
The measurement that filtrate described in embodiment 1 is carried out to protein and carbohydrate content, measures and adds 1% at 50 DEG C
Tween-80 after, self-dissolving 8h, content of soluble protein 7.97g/L, water-soluble carbohydrate content 8.50g/
L。
Embodiment 2
The fresh cyanobacteria mud of 1.0L is taken, the cyanobacteria that pH value is 7, moisture content is 95% is deployed into.Water bath with thermostatic control adds at 50 DEG C
Heat, spray plus the cationic surfactant cetyl trimethylammonium bromide solution 5ml of 0.2g/ml concentration are stirred evenly, i.e., this
When cetyl trimethylammonium bromide dosage be 2% of amount of dry matter in cyanobacteria solution, speed of agitator 80rpm, when reaction
Between 8h.Cyanobacteria solution is put into a centrifuge to be centrifugated 10min under 7000r/min revolving speed after reaction, obtains supernatant
Liquid.It is 0.45 μm of cellulose mixture microfiltration membranes, soluble protein and soluble carbon aquation in filtrate that supernatant, which is passed through aperture,
The content for closing object is the inclusion content that cyanobacteria self-dissolving releases after adding surfactant cetyl trimethylammonium bromide.
The measurement that filtrate described in embodiment 2 is carried out to protein and carbohydrate content, measures and adds 2% at 50 DEG C
Cetyl trimethylammonium bromide after, self-dissolving 8h, content of soluble protein 6.35g/L, soluble-carbohydrate contain
Amount is 6.75g/L.
Embodiment 3
The fresh cyanobacteria mud of 1.0L is taken, the cyanobacteria that pH value is 7, moisture content is 95% is deployed into.Water bath with thermostatic control adds at 55 DEG C
Heat, spray plus the anionic surfactant sodium dodecyl sulfate solution 5ml of 0.1g/ml concentration are stirred evenly, i.e., and at this time 12
Sodium alkyl sulfonate dosage is 1% of amount of dry matter in cyanobacteria solution.Speed of agitator is 80rpm, reaction time 4h.Reaction terminates
Cyanobacteria solution is put into a centrifuge to be centrifugated 10min under 7000r/min revolving speed afterwards, obtains supernatant.Supernatant is led to
Crossing aperture is 0.45 μm of cellulose mixture microfiltration membranes, and the content of soluble protein and soluble-carbohydrate is in filtrate
The inclusion content that cyanobacteria self-dissolving releases after addition surfactant sodium dodecyl base sodium sulfonate.
The measurement that filtrate described in embodiment 3 is carried out to protein and carbohydrate content, measures and adds 1% at 55 DEG C
Dodecyl sodium sulfate after, self-dissolving 4h, content of soluble protein 6.98g/L, water-soluble carbohydrate content is
7.55g/L。
Embodiment 4
The fresh cyanobacteria mud of 1.0L is taken, the cyanobacteria that pH value is 6, moisture content is 95% is deployed into.Water bath with thermostatic control adds at 45 DEG C
The nonionic surfactant Tween-80 solution 2.5ml of heat, spray plus 0.1g/ml concentration is stirred evenly, i.e., surface is living at this time
Property agent Tween-80 dosage be cyanobacteria solution in amount of dry matter 0.5%.Speed of agitator is 80rpm, reaction time 6h.
Cyanobacteria solution is put into a centrifuge to be centrifugated 10min under 7000r/mn revolving speed after reaction, obtains supernatant.It will be upper
Clear liquid is 0.45 μm of cellulose mixture microfiltration membranes by aperture, and soluble protein and soluble-carbohydrate contains in filtrate
Amount is the inclusion content that addition surfactant Polysorbate-cyanobacteria self-dissolving after 80s releases.
The measurement that filtrate described in embodiment 4 is carried out to protein and carbohydrate content, measures and adds at 45 DEG C
After 0.5% Tween-80, self-dissolving 6h, content of soluble protein 7.62g/L, water-soluble carbohydrate content is
8.16g/L。
Embodiment 5
The fresh cyanobacteria mud of 50.0L is taken, the cyanobacteria that pH value is 7, moisture content is 95% is deployed into.Uniformly spray plus 0.5g/ml are dense
The nonionic surfactant Tween-80 solution 0.5L of degree, i.e., -80 dosage of surfactant Polysorbate is indigo plant at this time
The 1% of amount of dry matter in algae solution.It is at the uniform velocity stirred using agitating paddle, 50 DEG C of heating, reaction time 8h in heating tank.Reaction knot
Cyanobacteria solution is put into disk centrifugal separator to be centrifugated 30min under 4000r/min revolving speed after beam, obtains supernatant.Supernatant
The content of soluble protein and soluble-carbohydrate is to add surfactant Polysorbate-cyanobacteria after 80s certainly in liquid
The molten inclusion content released.
The measurement that supernatant described in embodiment 5 is carried out to protein and carbohydrate content, measures and adds at 50 DEG C
After 1% Tween-80, self-dissolving 8h, content of soluble protein 6.88g/L, water-soluble carbohydrate content is
7.42g/L。
Comparative example 1
The fresh cyanobacteria mud of 1.0L is taken, the cyanobacteria that pH value is 7, moisture content is 95% is deployed into.Water bath with thermostatic control adds at 50 DEG C
Heat, speed of agitator 80rpm.It is sampled every 6h, sample is put into a centrifuge to be centrifugated under 7000r/min revolving speed
10min obtains supernatant.It is 0.45 μm of cellulose mixture microfiltration membranes, soluble protein in filtrate that supernatant, which is passed through aperture,
And the content of soluble-carbohydrate is content the amount of dissolution after Cells of Blue-green Algae broken wall.When the different self-dissolving times protein and
Carbohydrate the amount of dissolution is shown in Table 1.
Each index content under the different self-dissolving times in 1 comparative example 1 of table
| Detection project | 0h | 6h | 12h | 18h | 24h |
| Soluble protein (g/L) | 0.18 | 3.56 | 4.72 | 5.36 | 6.35 |
| Soluble-carbohydrate (g/L) | 0.53 | 3.63 | 5.52 | 7.20 | 7.40 |
Table 1 statistics indicate that: cyanobacteria needs 18-24h content burst size to can be only achieved stabilization under mesophilic condition, the time compared with
It is long, and soluble protein and water-soluble carbohydrate content are generally relatively low.
Comparative example 2
The fresh cyanobacteria mud of 100.0ml is taken, the cyanobacteria that pH value is 7, moisture content is 95% is deployed into.Ultrasonic wave is broken under ice bath
Wall 30min, ultrasonic power 800W.Cyanobacteria is put into centrifuge after broken wall, is centrifuged 10min under 7000r/min revolving speed.Take from
Supernatant obtained by the heart is 0.45 μm of cellulose mixture microfiltration membranes, soluble protein and soluble carbon aquation in filtrate by aperture
The content for closing object is content the amount of dissolution after Cells of Blue-green Algae broken wall.
The measurement that filtrate described in comparative example 2 is carried out to protein and carbohydrate content, measure ultrasonic wave 30min,
Under power 800W, content of soluble protein is 5.18g/L, water-soluble carbohydrate content 4.44g/L in cyanobacteria.
Comparative example 2 statistics indicate that: use supersonic wave wall breaking, Cells of Blue-green Algae content is adsorbed on cell wall, and far from super
The algae solution of sound column is broken not exclusively, causes soluble protein and water-soluble carbohydrate content lower.Therefore supercritical ultrasonics technology is broken
Wall energy consumption is high, and treating capacity is small.
Comparative example 3
The fresh cyanobacteria mud of 1.0L is taken, the cyanobacteria that pH value is 6, moisture content is 95% is deployed into.Water bath with thermostatic control adds at 50 DEG C
The sodium stearate solution 5ml of heat, spray plus 0.1g/ml concentration is stirred evenly, i.e., surfactant odium stearate dosage is at this time
The 1% of amount of dry matter in cyanobacteria solution.Speed of agitator is 80rpm, reaction time 6h.After reaction by cyanobacteria solution be put into from
To be centrifugated 10min under 7000r/min revolving speed in scheming, supernatant is obtained.It is 0.45 μm by aperture for supernatant to mix
Cellulose microfiltration membranes, the content of soluble protein and soluble-carbohydrate is to add the poly- mountain of surfactant in filtrate
The inclusion content that pear ester-cyanobacteria self-dissolving after 80s releases.
The measurement that filtrate described in comparative example 3 is carried out to protein and carbohydrate content, measures and adds 1% at 50 DEG C
Odium stearate after, self-dissolving 8h, content of soluble protein 4.95g/L, water-soluble carbohydrate content 5.38g/L.
Comparative example 3 statistics indicate that: use odium stearate surfactant, content discharge content it is lower, compared to addition
The optimal case of other surfaces activating agent, protein content is lower than protein content in preferred embodiment after adding odium stearate
38%, carbohydrate content lower than the content of preferred embodiment 37%.
For embodiment 1 compared in comparative example in other schemes, protein and carbohydrate content are as shown in table 2.
2 embodiment 1 of table and each index content of comparative example
Table 2 statistics indicate that: within the 8h reaction time, cyanobacteria using optimal case than use other methods discharge protein
It is dramatically increased with carbohydrate content.
Claims (6)
1. a kind of method for promoting the release of cyanobacteria content, it is characterised in that: cyanobacteria is carried out medium temperature self-dissolving processing, keeps cyanobacteria thin
Born of the same parents' broken wall, intracellular organic matter dissolution, and by adding surfactant, so that organic matter intracellular is obtained more efficient release.
2. a kind of method for promoting the release of cyanobacteria content according to claim 1, it is characterised in that: cyanobacteria moisture content exists
93%~95%.The temperature range of the medium temperature is 15-65 DEG C.
3. a kind of method for promoting the release of cyanobacteria content according to claim 2, it is characterised in that: the self-dissolving temperature
Degree is 45~55 DEG C.
4. a kind of method for promoting the release of cyanobacteria content according to claim 1, it is characterised in that: when the described self-dissolving
Between be 4~8h.
5. a kind of method for promoting the release of cyanobacteria content according to claim 1, it is characterised in that: the surface is living
Property agent includes dodecyl sodium sulfate, neopelex, cetyl trimethylammonium bromide, Tween-80 and ten
Six alkyl azochlorosulfonate propyl lycines it is one or more.
6. a kind of method for promoting the release of cyanobacteria content according to claim 1, it is characterised in that: the surface is living
Property agent dosage be cyanobacteria in dry biomass 0.5~2%.
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Application publication date: 20190510 |