CN1269889C - Composite chitin and its preparation method and application - Google Patents

Composite chitin and its preparation method and application Download PDF

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CN1269889C
CN1269889C CN 200510042036 CN200510042036A CN1269889C CN 1269889 C CN1269889 C CN 1269889C CN 200510042036 CN200510042036 CN 200510042036 CN 200510042036 A CN200510042036 A CN 200510042036A CN 1269889 C CN1269889 C CN 1269889C
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chitin
composite
composite chitin
molecular weight
aqua
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CN1654525A (en
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李向群
刘有利
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Ji'nan Appollo chitin Fertilizer Co.,Ltd.
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李向群
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Abstract

The present invention relates to a method for preparing composite chitin, which belongs to the technical field of biochemical industry and is used for the field of agriculture. The molecular weight of the composite chitin is distributed in the range of 200 to 100000, and the composite chitin is composed of chitin obtained by compounding oligosaccharide, oligosaccharide and polysaccharide in different molecular weight segments, and each component accounts for 0.1 to 95 wt%. The composite chitin of the present invention can be used as crop medical fertilizers, crop disease resisting and yield increasing agents or resistance inducing and yield increasing agents. Any poisonous and harmful components are not added into a producing and compounding process of the present invention, and the present invention is suitable for producing green and safe crops. The composite chitin is used for vegetables by adopting root irrigation or micro irrigation, drop irrigation, punching or leaf surface spraying, and is used for grain and fruits by adopting irrigation or leaf surface spraying according to a growth period.

Description

A kind of composite chitin and preparation method thereof and application
(1) technical field
The present invention relates to a kind of preparation method of composite chitin, belong to technical field of biochemical industry, be applied to agriculture field.
(2) background technology
As everyone knows, agricultural chemicals can effectively be controlled generation, the development of diseases and pests of agronomic crop, guarantees the good harvest of agricultural.According to statistics, China's agricultural chemicals year usage quantity uses agricultural chemicals can retrieve the crop yield loss of 15%-30% about 250,000 tons.But, if use agricultural chemicals improper, also can bring drug effect to be difficult for performance, destroy the agricultural ecological balance, side-effect problems such as pesticide residue in the increase agricultural-food and environmental pollution, severe patient also can cause poisoning to farm crop, makes the person poultry poisoning.
Human too busy to get away grain, and fertilizer is the grain of farm crop.Applying fertilizer is to improve crop yield, improves the important means of crop quality.According to statistics, the 40%-60% of general crop yield obtains by fertilising, but, because a large amount of uses of chemical fertilizer (China uses the chemical fertilizer total amount year at present about 4,000 ten thousand tons) and chemical fertilizer utilization ratio lower (average utilization is about 30%), cause the huge loss of chemical fertilizer and the raising of agricultural cost again, simultaneously, also cause the hardening soil, pollution of waterhead, soil ecology destroys, " dish is tasteless; melon is not fragrant; fruit is sweetless ", and nitrate, nitrite exceed standard in the agricultural-food, and nutritive value, economic worth reduce.
How science is used chemical pesticide, chemical fertilizer; how to seek fertile part chemical pesticide, the chemical fertilizer of substituting of non-harmful medicine, the protection environment is produced non-polluted farm product; green food, Organic food are especially one of developed country's problems of demanding urgently researching and developing of countries in the world.
1991, after the U.S., European physician found that chitin is the sixth-largest vital principle of human body, the research of chitin was very active.Preliminary from marine organisms chitin extraction and found that it is after the effect on the agricultural, we propose as give a definition: (1) fertilizer and soil conditioner: be used for keeping or improve plant nutrition and soil physics, chemical property, and biological epidemic various materials, can use separately or together; (2) fertilizer: so that plant nutrient to be provided is the material of its main effect; (3) agricultural chemicals: everyly be used to prevent and treat farm-forestry crop and products thereof insect, germ, weeds, preparation of harmful organisms such as bandicoot and plant-growth regulator, and auxiliary, synergistic agent of improving these pharmacy effects etc. all belong to the category of agricultural chemicals.From the efficiency analysis of chitin as can be known, chitin is the fertilizer that fertilizer neither be traditional, is the agricultural chemicals that agricultural chemicals neither be traditional.Particularly at the inducing anti-disease of having studied chitin, induce the root of the crop cell mitogenetic, improve crop yield, improve the mechanism of crop quality after, we think that chitin is a kind of halobios medicine fertilizer of novel combination of pesticide and fertilizer.
The composite chitin of different molecular weight section is being induced crop disease-resistant, is reducing the agricultural chemicals usage quantity, is improving crop yield, is improving crop quality, is being produced the function difference aspect the non-polluted farm product.Utilizing specified molecular weight section composite chitin production stable effect biological medicine fertilizer is one of current problem that needs to be resolved hurrily.
At present, the preparation method that chitin is commonly used both at home and abroad has:
1, chemical degradation method
With exsiccant shrimp, crab shell fragment with 3% salt acid soak 30h about, to not emitting till the bubble, water washes repeatedly to neutrality then, boil 1h with about 10% sodium hydroxide solution again, and the water flushing is to neutrality.Soak 1h with rare potassium permanganate solution, 70 ℃ of-80 ℃ of water-bath 30min of oxalic acid solution are washed to neutrality, and white chitin finished product is made in 105 ℃ of oven dry, pulverizing.
Chitin is placed on is incubated 1h (100 ℃~110 ℃) in the saturated sodium hydroxide solution, then washing.Handle in alkaline solution, washing 2~3 times repeatedly, promptly gets the higher chitosan of deacetylation behind the whole acetates except that the dereaction generation again.
2, mechanical degradation method
The crab shell is with 10% salt acid soak 3~4 days, and washing was boiled 1~2 hour with 10% sodium hydroxide again.Potassium permanganate solution decolouring is handled, drying, pulverize chitin.Microwave treatment 10 minutes is spent the night at room temperature condition.Washing again gets chitosan.
3, biological degradation method
Shrimp shell, crab shell be with 10% salt acid soak 3~4 days, and washing was boiled 1~2 hour with 10% sodium hydroxide again.Drying is pulverized chitin, adds enzyme then and degrades.Filter, drying gets chitin.
The weak point of the chitin of above-mentioned chemical degradation method, mechanical degradation method and biological degradation method production is that the range of molecular weight distributions of products obtained therefrom chitosan is narrower, be confined in certain scope, be generally 200-2000,2000-6000 and 6000-100000, the narrower chitosan of range of molecular weight distributions when using as biological medicine fertilizer, the effect instability.
(3) summary of the invention
At the deficiencies in the prior art, the invention provides a kind of composite chitin and preparation method thereof and application of molecular weight distribution broad.The chitosan that this method is produced with prior art is raw material, forms the composite chitin of molecular weight distribution broad, stable effect by compound being prepared into by the different molecular weight section.
Composite chitin of the present invention, in 200~100000 scopes, by the composite chitin that the chitin of different molecular weight section is formed, each weight percentages of components respectively accounts for 0.1~95% by oligosaccharide molecular amount 200-2000, oligosaccharides molecule amount 2000-6000 and the composite molecular weight distribution that obtains of polysaccharide molecular weight 6000-100000.
The preparation method of composite chitin, with oligosaccharide molecular amount 2002000, oligosaccharides molecule amount 2000-6000 and polysaccharide molecular weight 6000-100000, respectively account for by weight percentage 0.1~95% 20 ℃-80 ℃ down composite, obtain the composite chitin that molecular weight distribution is made up of the chitin of different molecular weight section in 200~100000 scopes.
Above-mentioned composite product can further transfer pH value to 9-10 with NaOH solution, place after 20-30 hour, the filtered and recycled throw out, throw out is extremely neutral with flushing with clean water, then in 50-70 ℃ of dryer, dry, 200~100000 composite chitins of forming by the different molecular weight section.
Above-mentioned oligosaccharide molecular amount 200-2000, oligosaccharides molecule amount 2000-6000 and polysaccharide molecular weight 6000-100000 are respectively the products of chemical degradation method in the prior art, mechanical degradation method and biological degradation method.
Above-mentioned oligosaccharide molecular amount 200-2000, oligosaccharides molecule amount 2000-6000 and polysaccharide molecular weight 6000-100000, be to be raw material greater than 100000 chitosan with molecular-weight average, the employing biological catalysis is degraded and is got, concrete grammar is as follows: with streptomyces (Streptomyces SP) bacterial classification is biological catalyst, the batch charging coefficient of chitosan raw material (raw material volume/reactor volume) is 0.4-0.6, and streptomyces bacterial classification concentration is 2.0 * 10 6~2.0 * 10 7Individual/ml, pH value 6-6.5, sealing was cultivated 1~10 day, and chitosan was degraded to oligosaccharide molecular amount 200-1500, oligosaccharides molecule amount 2000-6000 and polysaccharide molecular weight 3000-100000 for 20 ℃-50 ℃.
Used streptomyces (Streptomyces SP) bacterial classification CICC numbering 11010, purposes: malaga sugar isomerase.
Above-mentioned oligosaccharide molecular amount 200-2000, oligosaccharides molecule amount 2000-6000 and polysaccharide molecular weight 6000-100000, be to be raw material greater than 100000 chitosan with molecular-weight average, adopt chemical degradation method that the chitosan degraded is got, concrete grammar is: the hydrochloric acid soln that adds weight percent concentration 1~10% in the chitosan raw material, the weight ratio of chitosan raw material and 1~10% (WT) hydrochloric acid soln is 1: 20~500, sealing, under 10 ℃-90 ℃ condition, reacted 1~10 hour, chitosan is degraded to oligosaccharide molecular amount 200-2000, oligosaccharides molecule amount 2000-6000 and polysaccharide molecular weight 6000-100000.
Above-mentioned molecular-weight average is the chitosan product with prior art for preparing greater than 100000 chitosan.
The preparation method of composite chitin of the present invention, the molecular weight that chemical degradation method, mechanical degradation method, biological degradation method are obtained is greater than 30000 chitosan, adopt biological catalysis or chemical degradation method to continue degraded, make it become oligosaccharides (molecular weight 200~2000); Oligose (molecular weight 2000-6000); Polysaccharide (molecular weight 6000-100000), then that oligosaccharides, oligose and polysaccharide is compound, it is become have the composite chitin that molecular weight has 200-100000.Also oligosaccharides, oligose, the polysaccharide of chemical degradation method, mechanical degradation method, biological degradation method production directly can be combined into the composite chitin that the molecule amount is 200-100000.
Method of the present invention is easy to the composite chitin that large-scale production has oligosaccharides, oligose and polysaccharide recombination property.Produce in the recombination process and do not add any poisonous, objectionable constituent, be applicable to the production of green safety farm crop.
The composite chitin pulvis is a direct product of the present invention, the be weight percentage aqueous solution of 6% composite chitin of composite chitin aqua.
Composite chitin of the present invention is as farm crop medicine fertilizer, specifically as disease-resistant yield-increasing agent or inducing resistance yield promoter.Concrete grammar is as follows:
For vegetables: irritate root, slight irrigation, drip irrigation, dash and execute or foliage-spray, wherein, spray: the composite chitin aqua is converted water 10-200 kilogram for per 100 milliliters, and seedling stage, preceding, the young fruit expanding stage of blooming respectively spray 1-2 time.Irritate root: the composite chitin aqua is converted water 10-200 kilogram for per 100 milliliters, seedling stage, transplanting phase, filling root when root growth is bad.Dash and execute: mu consumption composite chitin aqua 100-1500 milliliter dashes with water to be executed.Composite chitin content is high, so its extension rate can not be low excessively, avoid producing and suppresses.
Foliage-spray, composite chitin aqua 1: 600-800 doubly should not be lower than 600 times.Directly absorb by the blade face, promote well developed root system.
Pouring (filling root), composite chitin aqua 1: 1000-1200 doubly should not be lower than 600 times.
For grain: jointing stage, heading stage, grouting initial stage composite chitin pulvis 2 grams or composite chitin aqua are converted water 1-100 kilogram for 20 milliliters, at each foliage-spray 1 time in three periods.
For fruit: budding period, preceding, the young fruit expanding stage of blooming, fruit color phase, ripe leaf fall period, composite chitin pulvis 2 grams or composite chitin aqua are converted water 1-100 kilogram for 20 milliliters.According to the fruit tree growing way, spray 4-5 time in whole growing, the budding period and bloom before can spray simultaneously in conjunction with urea.Or select emphasis to use period according to the cultivation features of different fruit trees.
(4) embodiment
Embodiment 1:
1, degraded
With molecular-weight average greater than 100000 chitosan and streptomyces bacterial classification concentration 2.0 * 10 6The biological catalyst of individual/ml places reactor, pH value 6-6.5, batch charging coefficient 0.4, sealing.25 ℃-28 ℃ of culture temperature were cultivated 3 days, respectively took out 1/3rd culture material respectively at the 3rd day, the 4th day, the 5th day, and they are respectively oligosaccharide molecular amount 200-1000, oligosaccharides molecule amount 2000-6000 and polysaccharide molecular weight 6000-100000.
2, composite
With 30%, 40%, 40% mixing under 0~70 ℃ by weight percentage of three times culture materials, transfer pH value to 10 with 40% (WT) NaOH solution, place after 24 hours, the filtered and recycled throw out, throw out is extremely neutral with flushing with clean water repeatedly, then in 60 ℃ of dryers, dry, promptly get 200~100000 composite chitins of forming by the different molecular weight section.
The composite chitin of embodiment 1 preparation has the effect of disease-resistant yield-increasing, and application method and effect are as follows:
500 times of (1) 6000 times of composite chitin pulvis and Lei Duo Mils alternately spraying+macroelement liquid fertile 30 kilograms/mu towards executing; , odd number is sprayed microbial inoculum (the many Mils of thunder), even number time spray composite chitin pulvis, totally 2 times/month.
(2) humic acid liquid fertilizer dashes for 50 kilograms/mu and executes+500 times of the many Mils of thunder and the fertile 500 times of sprayings of macroelement liquid 4 times/month.
(3) blank (do not apply fertilizer, not dispenser, water, water gaging such as spray).4 times/month.
Prevent and treat the oidium effect and see Table 1:
Table 1
Figure C20051004203600061
As can be seen from Table 1, it is effective to handle (1) control cucumber downy mildew, and preventive effect reaches 82.5%, and (2) drug effect is suitable with handling, and difference is not remarkable.
Effect of increasing production sees Table 2:
Table 2
Handle Output (kilogram) Volume increase (%) Volume increase The leaf look Vane thickness The stipes rugosity Stipes length The fruit type Fruit colour
(1) 56 55.6 10.9 Dark green Thick Sturdy No significant difference Along bar Normally
(2) 50 40.2 -- Dark green Thick Sturdy No significant difference Along bar Normally
(3) 36 -- -- Pale green Difference Difference -- Difference Normally
Annotate: output is the output of 40 square metres of commodity melons.
As can be seen from the above table, handle (1) rate ratio blank (3) volume increase 55.6%, comparison is according to (2) volume increase 10.9%, and is than its leaf look more dark green with blank, blade thickness, and cane is sturdy, and fruit (melon) is along bar.
Embodiment 2:
1, degraded
Molecular-weight average is put into reactor greater than 100000 chitosan, add 10% hydrochloric acid soln simultaneously, weight ratio 1: 100, sealing.React under 70 ℃-75 ℃ condition, respectively take out 1/3 reactant respectively at 1 hour, 2 hours, 4 hours, they are respectively oligosaccharide molecular amount 200-1500, oligosaccharides molecule amount 2000-6000 and polysaccharide molecular weight 6000-100000.
2, composite
0~70 ℃ of mixing of reactant with top three taking-ups, transfer pH value to 10 with 40% (WT) NaoH solution, place after 24 hours, filter, reclaim throw out, with throw out repeatedly with flushing with clean water to neutral, then 55 ℃ of oven dry, promptly get molecular weight and be the composite chitin of 200~100000 different molecular weight section.
The composite chitin of embodiment 2 preparations has the effect that induction of resistance increases output, and application method and effect are as follows:
Results from pot experiment test (seeing Table 3) shows, the preventive effect of 400 times, 500 times, 600 times liquid inducing watermelon anti-blights in seedling stage of composite chitin aqua increases with concentration and increases progressively.The prevention effect of three concentration is respectively 63.03%, 49.26%, 34.14%, and its preventive effect of 400 times significantly is better than its preventive effect of 600 times, and extremely significantly is better than the preventive effect (18.94%) of standard control yellow humic acid; The disease of each concentration of treatment refers to be 25.94-56.90, all extremely significantly is lower than to connect bacterium contrast disease and refer to 70.19.
Table 3 composite chitin aqua inducing watermelon anti-blight test-results
Handle Concentration (doubly) Total strain Repeat Diseased plant rate (%) Disease refers to Preventive effect (%)
The composite chitin aqua 400 10 8 9 8 35 1234 is flat 100 100 100 100 100 28.57 25.00 26.98 23.21 25.94dD 59.30 64.38 61.52 66.93 63.03aA
The composite chitin aqua 500 9 8 10 9 36 1234 is flat 100 100 100 100 100 39.68 39.29 42.86 20.63 35.62cdCD 43.47 44.02 38.94 70.01 49.26abAB
The composite chitin aqua 600 13 14 13 13 53 1234 is flat 100 100 100 100 100 57.14 51.02 36.26 40.48 46.23bcBC 18.59 27.31 48.34 42.33 34.14bcBC
The yellow humic acid aqua 400 15 15 13 16 59 1234 is flat 100 100 100 100 100 42.86 58.10 65.93 60.71 56.90bAB 38.94 17.22 6.07 13.51 18.91cB
CK - 15 13 14 14 56 1234 is flat 100 100 100 100 100 63.81 80.22 71.43 65.31 70.19aA
The composite chitin aqua promotes growth effect measurement result (seeing Table 4) to show to watermelon seedling stage, the composite chitin aqua is except that inducing watermelon anti-blight in seedling stage, the effect that also has obvious promotion plant strain growth, 400, the long and single-strain fresh weight of 500,600 times of plant height, roots of handling butt joint bacterium watermelon seedlings increases with concentration and increases progressively.The plant height that three concentration is handled and the standard control yellow humic acid is handled is respectively 56.84cm, 54.64cm, 49.78cm and 50.94cm, and all the utmost point is significantly higher than and connects bacterium and contrast plant height (42.03cm), all significantly is lower than the plant height (66.3cm) of blank.The root length of three concentration is respectively 7.88cm, 7.21cm, 6.18cm, and the long and blank root long (8.05cm) of wherein 400 times, 500 times root is the utmost point root long (5.66cm and 5.12cm) that is significantly higher than its 600 times and yellow humic acid and connects the bacterium contrast all.The single-strain fresh weight of three concentration is respectively 3.67g, 3.18g and 2.08g, and wherein 400 times and single-strain fresh weight blank (3.80g) be the utmost point single-strain fresh weight (2.42g and 1.88g) that is significantly higher than its 500 times, 600 times and yellow humic acid and connects the bacterium contrast all.
Table 4 composite chitin aqua is to the promotion growth exercising result of watermelon seedling
Handle Repeat Total strain Plant height (cm) Rate of increase (%) Root long (cm) Rate of increase (%) Single-strain fresh weight (g) Rate of increase (%)
400 times in composite chitin aqua 1 11 57.94 37.85 8.59 61.39 3.79 102.13
2 13 58.23 38.54 7.52 41.29 3.65 94.67
3 12 52.20 24.20 7.38 38.66 3.80 102.67
4 11 59.00 40.38 8.03. 50.87 3.42 82.4
Flat 47 56.84bAB 35.24 7.88abA 48.05 3.67aA 95.47
500 times in composite chitin aqua 1 12 59.12 40.66 8.11 52.37 3.32 77.07
2 16 53.74 27.86 6.08 14.23 2.97 58.40
3 14 50.24 19.53 7.28 36.78 3.20 70.67
4 11 55.46 31.95 7.36 38.28 3.23 72.27
Flat 53 56.64bB 30.00 7.21bA 35.42 3.18bB 69.6
600 times in composite chitin aqua 1 15 48.07 14.37 6.52 22.50 1.92 2.4
2 14 48.89 16.32 6.20 16.49 1.88 0.27
3 9 50.00 18.96 6.19 16.30 2.20 17.33
4 10 52.17 24.13 5.81 9.16 2.33 24.27
Flat 48 49.78bBC 18.45 6.18cB 16.11 2.08dCD 11.07
Meet bacterium CK 1 24 45.11 - 5.09 - 1.92 -
2 12 39.81 - 4.96 - 2.03 -
3 15 47.05 - 5.32 - 1.85 -
4 12 36.15 - 5.11 - 1.70 -
Flat 63 42.03cC - 5.12dC - 1.88dD -
Blank CK 1 20 55.02 30.91 8.38 57.45 3.70 97.33
2 6 67.55 60.72 7.84 47.30 3.82 103.73
3 4 72.33 72.09 7.53 41.48 3.9 108.00
4 9 70.35 67.38 8.45 58.76 3.77 101.07
Flat 39 66.31aA 57.77 8.05aA 51.24 3.80aA 102.53
Embodiment 3:
1, with molecular-weight average greater than 100000 chitosan and streptomyces bacterial classification concentration 2.0 * 10 7The biological catalyst of individual/ml places reactor, pH value 6-6.5, batch charging coefficient 0.6, sealing.25 ℃-28 ℃ of culture temperature were cultivated 4 days, respectively took out 1/3rd culture material respectively at the 4th day, the 5th day, the 6th day, and they are respectively oligosaccharide molecular amount 200-2000, oligosaccharides molecule amount 2000-6000 and polysaccharide molecular weight 6000-30000.
2, three times culture material 15%, 42.5%, 42.5% is mixed by weight percentage, transfer pH value to 10 with 40% (WT) NaOH solution, place after 24 hours, the filtered and recycled throw out, throw out is extremely neutral with flushing with clean water repeatedly, then in 65 ℃ of dryers, dry, promptly get 200~100000 composite chitins of forming by the different molecular weight section.
Use the composite chitin of embodiment 3 preparations to have to increase output, promote well developed root system, improve the effect of quality, application method and effect see Table 5-7, wherein A: composite chitin aqua, B: composite chitin pulvis, C: potassium primary phosphate+naphthylacetic acid, CK: contrast.
Table 5
Each handles table 6 the blocky influence of strawberry
Handle Repeat On average With CK contrast CK (%)
I II III
A B C CK 90.6 88.5 100.0 92.9 88.7 91.7 96.5 82.3 90.3 89.4 94.7 86.4 89.8 89.7 95.4 87.2 102.98 102.67 109.4 100
The influence of table 7 pair strawberry interior quality
Quality parameter Handle
A B C CK
Hardness soluble solid titratable acid total reducing sugar 0.57 8.94 1.22 8.23 0.59 8.94 1.25 8.34 0.49 8.63 1.18 8.15 0.55 8.55 1.15 8.12
Embodiment 4:
To directly be re-dubbed the chitin biotechnological formulation that molecular weight is 200-30000 under the oligosaccharides of prior art chemical degradation method, mechanical degradation method, biological degradation method production, oligose, the polysaccharide room temperature, the weight percent of oligosaccharides, oligose and polysaccharide is 1%, 37% and 62%.
Use the composite chitin of embodiment 4 preparations to have the effect that increases output, improves quality, specifically test as follows:
Table 8 tomato is the quantity recorder as a result
I II III
The composite chitin aqua 3.86 3.44 1.88 1.80 3.76 3.38 2.40 1.48 3.9 3.38 2.56 1.61
The composite chitin pulvis 4.04 3.63 2.60 1.64 3.82 3.52 2.32 1.42 4.02 3.47 2.46 1.68
Contrast 3.70 3.18 2.12 1.40 3.52 3.27 1.96 1.28 3.52 3.27 1.95 1.28
Annotate: 1. 2. 3. 4. represent one, two, three, four fringes fruit
Table 9 tomato per plant quantity statistics table
Add up to Increase number
The composite chitin aqua 3.84 3.40 2.28 1.63 11.15 110
The composite chitin pulvis 3.96 3.54 2.46 1.58 11.54 114
Contrast 3.58 3.24 2.01 1.32 10.15 100
Annotate: 1. 2. 3. 4. represent one, two, three, four fringes fruit.
Table 9 shows that composite chitin aqua, composite chitin pulvis per plant quantity compares respectively according to increasing by 10%, 14%.The tomato production statistical study sees Table 10:
Table 10 tomato cell production cartogram
Use the tomato of aforesaid method plantation to see Table 11 through Ministry of Agriculture food quality supervision verification test center (Jinan) detected result:
Table 11
Sample number into spectrum The sample title The sample proterties Test item
Total acid (%) Vitamins C (mg/100g) Total reducing sugar (%)
2001-X-385 Tomato (contrast) Bright 0.38 14.3 3.34
2001-X-386 Tomato (aqua) Bright 0.43 16.1 3.38
2001-X-387 Tomato (pulvis) Bright 0.44 15.3 3.72

Claims (9)

1, a kind of composite chitin, it is characterized in that by oligosaccharide molecular amount 200~2000, oligosaccharides molecule amount 2000~6000 and polysaccharide molecular weight 6000~100000 composite molecular weight distribution that obtain each weight percentages of components respectively accounts for 0.1~95% in 200~100000 scopes, by the composite chitin that the chitin of different molecular weight section is formed.
2, the preparation method of the described composite chitin of claim 1, it is characterized in that oligosaccharide molecular amount 200~2000, oligosaccharides molecule amount 2000~6000 and polysaccharide molecular weight 6000~100000, respectively account for by weight percentage 0.1~95% 20 ℃~80 ℃ down composite, obtain the composite chitin that molecular weight distribution is made up of the chitin of different molecular weight section in 200~100000 scopes.
3, the preparation method of composite chitin as claimed in claim 2, it is characterized in that described oligosaccharide molecular amount 200~2000, oligosaccharides molecule amount 2000~6000 and polysaccharide molecular weight 6000~100000, be to be raw material greater than 100000 chitosan with molecular-weight average, the employing biological catalysis is degraded and is got, concrete grammar is: with the streptomyces bacterial classification is biological catalyst, the batch charging coefficient of chitosan raw material is 0.4~0.6, and streptomyces bacterial classification concentration is 2.0 * 10 6~2.0 * 10 7Individual/ml, pH value 6~6.5, sealing was cultivated 1~10 day for 20 ℃~50 ℃, and the chitosan degraded is got.
4, the preparation method of composite chitin as claimed in claim 2, it is characterized in that described oligosaccharide molecular amount 200~2000, oligosaccharides molecule amount 2000~6000 and polysaccharide molecular weight 6000~100000, be to be raw material greater than 100000 chitosan with molecular-weight average, adopt chemical degradation method that the chitosan degraded is got, concrete grammar is: the hydrochloric acid soln that adds weight percent concentration 1~10% in the chitosan raw material, the weight ratio of chitosan raw material and 1~10%WT hydrochloric acid soln is 1: 20~500, under 10 ℃~90 ℃ condition, reacted 0.5~10 hour, the chitosan degraded is got.
5, the application of the described composite chitin of claim 1 is as farm crop medicine fertilizer.
6, the application of composite chitin as claimed in claim 5 is characterized in that as disease-resistant crops volume increase agent or inducing resistance yield promoter.
7, the application of composite chitin as claimed in claim 5 is characterized in that adopting filling root or slight irrigation, drip irrigation, dashing and execute or foliage-spray for vegetables; Wherein, spray: the composite chitin aqua is converted 10~200 kilograms in water for per 100 milliliters, and seedling stage, preceding, the young fruit expanding stage of blooming respectively spray 1~2 time; Irritate root: the composite chitin aqua is converted 10~200 kilograms in water for per 100 milliliters, seedling stage, transplanting phase, filling root when root growth is bad; Dash and to execute: 100~1500 milliliters in mu consumption composite chitin aqua dashes with water and executes; The be weight percentage aqueous solution of 6% composite chitin of described composite chitin aqua.
8, the application of composite chitin as claimed in claim 5 is characterized in that converting 1~100 kilogram in water for grain for 20 milliliters at jointing stage, heading stage, grouting initial stage composite chitin pulvis 2 grams or composite chitin aqua, at each foliage-spray 1 time in three periods; The be weight percentage aqueous solution of 6% composite chitin of described composite chitin aqua.
9, the application of composite chitin as claimed in claim 5, it is characterized in that spraying 4~5 times in budding period, preceding, the young fruit expanding stage of blooming, fruit color phase, ripe leaf fall period whole growing for fruit, composite chitin pulvis 2 gram or composite chitin aqua are converted 1~100 kilogram in water for 20 milliliters, the budding period and bloom before spray simultaneously in conjunction with urea; The be weight percentage aqueous solution of 6% composite chitin of described composite chitin aqua.
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CN103371092A (en) * 2012-04-28 2013-10-30 谷尚昆 Method for applying chitin solution (powder), chitosan solution (powder) and chitosan solution (powder) nutrient solutions to water (mist) fruit and vegetable cultivation
CN103718803B (en) * 2013-12-23 2015-09-16 广西南宁碧湾园林工程有限公司 A kind of chitin promotes the method that vegetable seedling is taken root
CN103960240A (en) * 2014-05-19 2014-08-06 江苏徐淮地区淮阴农业科学研究所 Watermelon yield increasing and sugar increasing conditioner and use method thereof
CN107624792A (en) * 2017-09-22 2018-01-26 浙江海洋大学 A kind of method that plant immune inducer is prepared using crab shell

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