CN1262519C - Industrialization manufacturing method of double effect composite microbial manure - Google Patents
Industrialization manufacturing method of double effect composite microbial manure Download PDFInfo
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- CN1262519C CN1262519C CN 200410050683 CN200410050683A CN1262519C CN 1262519 C CN1262519 C CN 1262519C CN 200410050683 CN200410050683 CN 200410050683 CN 200410050683 A CN200410050683 A CN 200410050683A CN 1262519 C CN1262519 C CN 1262519C
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Abstract
The present invention relates to an industrialized manufacturing method of a double-effect composite microbial manure. Firstly, a glutinous bacillus strain is cultured and activated by one to three stages of fermentation culture through a culture medium; a bacterial liquid is adsorbed by stirring with danty powder to form a glutinous bacillus bacterial agent; a streptomycete strain is cultured and activated by one to three stages of fermentation culture through a culture medium; a fermentation liquid is adsorbed by stirring with danty powder to form a streptomycete bacterial agent; danty, bone meal, monoammonium phosphate, urea, potassium sulfate, zinc sulfate, borax, magnesium sulfate, manganese sulphate, cupric sulfate, ferrous sulfate heptahydrate, sodium molybdate, the glutinous bacillus bacterial agent and the streptomycete bacterial agent are prepared proportionally to obtain the double-effect composite microbial manure. In the present invention, a biological engineering technology is used to produce the bacterial agents by biologically fermenting microbial strains with the functions of depotassium, dephosphorus and nitrogen fixation and an inhibition function on various disease fungus, and the double-effect composite microbial manure is compounded with a high-quality organic matter as a carrier. The present invention has the advantages that nutrients with quick action and long action are combined, the requirements of agronomic crops for nutrients can be met by once fertilizer application, the occurrence of diseases can be prevented or reduced, the increase effect in yield is obvious, and the quality of agricultural products is enhanced.
Description
Technical field
The present invention relates to a kind of composite microbial manure, particularly a kind of industrialized preparing process of fertile medicine unification type economic benefits and social benefits composite microbiological fertilizer of the control that can be used as plant growing process nutrient supply and disease.
Technical background
Fertilizer commonly used in the known modern agriculture production has chemical fertilizer, organic fertilizer and bio-feritlizer three major types.Chemical fertilizer mostly is Industrial products, available nutrient content is higher, is applied on the farm crop instant effect, because composition is more single, fertilizer efficiency is short again, easily causes farm crop nutrient deficiency and later stage to take off fertilizer and cause the underproduction, simultaneously, progress along with development of global economy and society, people are more and more higher to the requirement of quality of life and Food Quality, thirst for obtaining good, the eutrophic food of mouthfeel, pursue pure natural, free of contamination heath food.But the excessive exploitation of agricultural is executed list to fertilize especially for a long time partially, causes soil quality to descend, and Soil degradation is serious, finally influences the production of high quality agricultural products.Contain abundant in organic fertilizer and the bio-feritlizer machine matter and useful soil microorganisms, after being manured into soil, can improve the soil, culture fertility, improve the surge capability of the feeder capability and the raising soil of soil, but the total nutrient content of organic fertilizer and bio-feritlizer is lower, and fertilizer efficiency is slow, makes personage's early growth period nutrient supply not enough and cause the underproduction.Be used as the agricultural chemicals of preventing and treating disease in the known agriculture production and mostly be chemosynthesis,, use that pesticide residue exceeds standard in the agricultural-food of back, be detrimental to health, and soil, water quality and the environment mankind are all caused in various degree pollution though certain effect is arranged.The biotechnological formulation product of the last seed manure medicine unification type to be supplied in market promptly satisfies the supply of required nutrient of crop growth phase, can prevent and treat disease again, and toxicological harmless and pollution.
Summary of the invention
The industrialized preparing process that the purpose of this invention is to provide a kind of economic benefits and social benefits composite microbiological fertilizer, product is nuisanceless and have nutrient supply and disease control double effects, and is easy to use.
For achieving the above object, the present invention is according to potassium decomposing, phosphorus decomposing, nitrogenase activity and streptomycete meta-bolites---the antagonistic action of Polyoxin of colloid bacillus cereus, pass through biological fermentation, with the carrier of high-quality organic matter as zymocyte liquid, according to plant growing process to nutrient demand, scientific and reasonable formulated.
Production method provided by the invention is as follows:
1. the preparation of colloid bacillus cereus microbial inoculum (A microbial inoculum): elder generation is with the bacterial strain of original preservation, being seeded to nitrogen-free agar activates, 27 ℃-29 ℃ constant temperature culture 3-4 days, after treating that lawn covers with, produce bacterial classification, then this bacterial classification is inoculated in by the part by weight of substratum charging capacity 5% and carries out one grade fermemtation in the first class seed pot and cultivate, the tank body volume is 100 liters, substratum is with improveing Ah Xu shellfish substratum, press after 24 hours to plant to the secondary seed jar and carry out the second order fermentation cultivation, the tank body volume is 1000 liters, substratum is the same, pressed to plant after 24 hours again and carry out three grade fermemtation to the three grade fermemtation jar, and the tank body volume is 10000 liters, substratum starch ammonium, pH value are 7.2-7.4; Stop fermentation after 36 hours, with bacterium liquid with the part by weight whip attachment of weathered coal powder by 1: 5, the colloid bacillus cereus microbial inoculum.
2. the preparation of drappus microbial inoculum (B microbial inoculum): with the bacterial strain of original preservation, be seeded to slant medium and carry out activation culture, 28 ℃-29 ℃ constant temperature culture 5-6 days, treat that spore covers with after, produce bacterial classification; Then this bacterial classification is inoculated in by the part by weight of substratum charging capacity 5% and carries out one grade fermemtation in the first class seed pot, the tank body volume is that 100 liters after 48 hours are pressed bacterial classification and kind carried out second order fermentation to the secondary seed jar, and the tank body volume is 1000 liters; Press after 72 hours to plant and carry out three grade fermemtation to the three grade fermemtation jar, the tank body volume is 10000 liters; Above-mentioned one, two, three the general fermention medium visible mycelia of microscopically fracture after 48 hours of fermenting, the thalline self-dissolving, and detect the tiring when reaching the 1400-1500 potency unit of mycins of supporting more, can stop fermentation; Through Plate Filtration, its filtrate and weathered coal powder are pressed the part by weight of 1:6 with fermented liquid, whip attachment, drappus microbial inoculum.
3. the preparation of economic benefits and social benefits composite microbiological fertilizer, prepare by following weight ratio of constituents example:
Weathered coal 25-30%
Bone meal 8-10%
Monoammonium phosphate 8-10%
Urea 20-24%
Vitriolate of tartar 12-15%
Zinc sulfate 0.3-0.5%
Borax 0.3-0.5%
Sal epsom 0.030.05%
Manganous sulfate 0.030.05%
Copper sulfate 0.003-0.005%
Ferrous sulfate 0.003-0.005%
Sodium orthomolybdate 0.003-0.005%
A microbial inoculum 3-5%
B microbial inoculum 3-5%
Wherein A microbial inoculum, B microbial inoculum are packed separately respectively, and other composition is hybrid packed.
Advantage of the present invention is: the applied bioengineering technology will have potassium decomposing, phosphorus decomposing, the colloid bacillus cereus of fixed nitrogen function is made microbial inoculum with the streptomycete with the multiple pathogenic fungi function of inhibition by biological fermentation, with the high-quality organic matter is the synthetic product of the present invention of carrier, effectively promote the conversion of nutritive element in the soil, improve crop absorbing to nutrition, its meta-bolites also has the plant growth of stimulation effect, colloid bacillus cereus and streptomycete be breeding in a large number in soil, be formed with bacteria group, created good rhizosphere ecotope, can effectively improve the constructional aspect of soil, culture fertility, make the good water of Soil conservation, fertile, gas, heat condition, can make the deleterious pathogenic bacteria of crop is inhibited, thereby prevent and reduced the generation of disease.Simultaneously, the meta-bolites of streptomycete fermentation in the product a---Polyoxin is a kind of broad spectrum antibiotic, most pathogeny fungies all there is stronger restraining effect, the distinctive anti-smelting disease function of the present invention, the various fertilizer that are present farm crop widespread use are not available, and, physical environment and people's health is guaranteed to soil, water quality and environment toxicological harmless, noresidue, pollution-free.Quick-acting and the long-acting nutrient of product of the present invention combines, accomplish single fertilization, can satisfy the demand of farm crop whole growth phase to nutrition, prevent or reduced the generation of disease again, not only easy to use, can also reduce input cost simultaneously, simultaneously obvious effect of increasing production, amount of increase in production can reach 20-30%, and can improve the quality of agricultural-food.
Embodiment
Concrete production method is as follows:
The source of original strain: colloid bacillus cereus original strain (Bacillus mucilaginosusKrassilnikov Accc10012) and streptomycete original strain (Streptomyces aureusWaksmanet Gurtis CFcc3010) are the preservation of Beijing DSMZ of institute of microbiology, get superior strain behind mutagenesis screening.
1, the preparation of bacterial classification is produced in colloid bacillus cereus agent (A microbial inoculum): the colloid bacillus cereus bacterial strain with original preservation, be seeded on the nitrogen-free agar, 27 ℃-29 ℃ constant temperature culture 3-4 days, treat that lawn covers with after, can become to produce bacterial classification.
Nitrogen-free agar weight ratio of constituents example:
Sucrose 10 grams
Dipotassium hydrogen phosphate 0.2 gram
Sal epsom 0.2 gram
Yellow soda ash 0.2 gram
Vitriolate of tartar 0.1 gram
Zinc sulfate 0.05 gram
Iron trichloride 0.05 gram
Lime carbonate 5 grams
Agar 20 grams
Water 1000ml
PH 7.2-7.4
2, the fermentative production of bacterial classification:
The first order seed fermentation: with above-mentioned cultured bacterial classification, be inoculated in the first class seed pot, carry out one grade fermemtation, the tank body volume is 100 liters.Fermentation condition: 70 liters of substratum charging capacitys, adopt improvement Ah Xu shellfish substratum, inoculum size is by substratum 5% of the part by weight that feeds intake, 28 ℃ of leavening temperatures, 240-250 rev/min of stirring revolution.1.2 cubic metres/hour of air flows, fermentation time 24 hours.
The secondary seed fermentation: the bacterium liquid with one grade fermemtation finishes, be seeded in the secondary seed jar by pressing kind of a pipe, carry out second order fermentation, the tank body volume is 1000 liters.Fermentation condition: substratum charging capacity 700 liters, adopt improvement Ah Xu shellfish substratum, 28 ℃ of leavening temperatures stir 240-250 rev/min of revolution.12 cubic metres/hour of air flows, fermentation time 24 hours.
Three grade fermemtation: the bacterium liquid with second order fermentation finishes, be seeded in the three grade fermemtation jar by pressing kind of a pipe, carry out three grade fermemtation, the tank body volume is 10000 liters.Fermentation condition: substratum charging capacity 7000 liters, adopt starch ammonium substratum, 28 ℃ of leavening temperatures stir 240250 rev/mins of revolutions.120 cubic metres/hour of air flows, fermentation time 36 hours.
Improvement Ah Xu shellfish medium component part by weight:
Sucrose 1%
Dipotassium hydrogen phosphate 0.02%
Sal epsom 0.02%
Yellow soda ash 0.02%
Calcium sulfate 0.01%
Lime carbonate 0.5%
Vitriolate of tartar 0.0%
Zinc sulfate 0.05%
Iron trichloride 0.05%
PH 7.2-7.4
Starch ammonium medium component part by weight:
Sucrose: 0.2%
Starch 0.5%
Dipotassium hydrogen phosphate 0.2%
Sal epsom 0.05%
Yellow soda ash 0.02%
Ammonium sulfate 0.1%
Zinc sulfate 0.05%
Lime carbonate 0.1%
Yeast extract paste 0.02%
Peptone 0.01%
Iron trichloride 0.001%
PH 7.2-7.4
3, the preparation of colloid bacillus cereus microbial inoculum (A microbial inoculum):
The bacterium liquid that stops fermentation is discharged by discharge port, with the part by weight whip attachment of weathered coal powder by 1: 5, the colloid bacillus cereus microbial inoculum.
4, the preparation of streptomyces species: with the streptomycete bacterial strain of original preservation, be seeded on the slant medium, 28 ℃-29 ℃ constant temperature culture 5-6 days, after treating that spore covers with, be seeded in the fermention medium 28 ℃ of shaking culture 72 hours, there is not assorted bacterium through microscopy, and thalline is plentiful, and mycelia is sturdy, can make fermented bacterium.
Slant medium weight ratio of constituents example:
Saltpetre 0.1%
Dipotassium hydrogen phosphate 0.05%
Sal epsom 0.05%
Sodium-chlor 0.05%
Starch 2%
Ferrous sulfate 0.01%
PH 7.0-7.2
Fermention medium weight ratio of constituents example:
Semen Maydis powder 2%
Bean powder 2%
Maltose 1%
Yeast extract paste 0.5%
Dipotassium hydrogen phosphate 0.2%
Sal epsom 0.05%
Sodium-chlor 0.2%
Zinc sulfate 0.5%
Lime carbonate 0.2%
Copper sulfate 0.005%
Manganous sulfate 0.005%
Ferrous sulfate 0.005%
Peptone 0.01%
PH 7.0-7.2
5, the fermentative production of streptomyces species:
One grade fermemtation: with above-mentioned cultured liquid spawn, be inoculated in the first class seed pot, carry out one grade fermemtation, the tank body volume is 100 liters.Fermentation condition: charging capacity 70 liters, adopt fermention medium, inoculum size is by substratum 5% of the part by weight that feeds intake, and 8 ℃ of leavening temperatures stir 220-230 rev/min of revolution, 1.85 cubic metres/hour of air flows, fermentation time 24-36 hour.
Second order fermentation: the bacterium liquid that one grade fermemtation is finished is seeded in the second order fermentation jar by pressing kind of a pipe, and the tank body volume is 1000 liters.Fermentation condition: charging capacity 700 liters, adopt fermention medium, 28 ℃ of leavening temperatures stir 220-230 rev/min of revolution, 18.5 cubic metres/hour of air flows, fermentation time 24-36 hour.
Three grade fermemtation: the bacterium liquid that second order fermentation is finished when not having assorted bacterium through microscopy, is seeded in three grades of seeding tanks by pressing a kind pipe, carry out three grade fermemtation, the tank body volume is 10000 liters, stirs 220-230 rev/min of revolution, 185 cubic metres/hour of air flows, fermentation time 72-80 hour.When the meta-bolites of measuring streptomyces aureus, promptly tiring of Polyoxin reaches 1400-1500 unit, stops fermentation.
6, the preparation of drappus microbial inoculum (B microbial inoculum): the bacterium liquid that will stop fermentation is discharged by discharge port, with the weathered coal powder by 1: 6 part by weight whip attachment, drappus microbial inoculum.
7, weathered coal pre-treatment:
The weathering coal dust of buying (content of humic acid 40-50%, fineness are 40 orders) is mixed with the part by weight of bicarbonate of ammonia by 5: 1, add water while stirring, amount of water is 10%, the rear enclosed that is mixed carries out aminating reaction, temperature is 25 ℃, needs 10 days, and needed get final product winter in 15-20 days.
8, the pre-treatment of bone meal:
The bone meal (fineness is 40 orders) bought and the part by weight of 25% dilute sulphuric acid by 1: 0.8 are mixed, be mixed after 24 hours acidification reactions, available phosphorus contents can reach 8%-10%.
9, the economic benefits and social benefits composite microbiological fertilizer is prepared by following weight ratio of constituents example:
Weathered coal 25-30%
Bone meal 8-10%
Monoammonium phosphate 8-10%
Urea 20-24%
Vitriolate of tartar 12-15%
Zinc sulfate 0.3-0.5%
Borax 0.30.5%
Sal epsom 0.03-0.05%
Manganous sulfate 0.03-0.05%
Copper sulfate 0.003-0.005%
Ferrous sulfate 0.003-0.005%
Sodium orthomolybdate 0.003-0.005%
A microbial inoculum 5%
B microbial inoculum 5%
Wherein A microbial inoculum, B microbial inoculum are packed separately respectively, and other composition is hybrid packed.
10, packing: in view of two kinds of functional characteristicss that the function microbial inoculum is different separately among the present invention, and the handiness of application process, especially guarantee its effective active, thus A, the independent packaged form of B microbial inoculum adopted, existing with now mixing.Total amount accounts for 10% altogether, and wherein the A microbial inoculum 5%, B microbial inoculum 5%.
The key technical indexes of product of the present invention
Available nitrogen content 12%
Available phosphorus content 〉=6%
Effective potassium content 〉=7%
Organic content 〉=40%
Microorganism viable bacteria number 〉=5,000,000/gram
Streptomycete is tired 〉=700 potency units
The using method of product of the present invention: use preceding A, B and now mix with existing, adding suitable quantity of water during mixing then can be more even.
1, can make base fertilizer, amount of application 20-30 kilogram/mu is in being manured into soil during whole ground.
2, can do and topdress, the crop whole growth phase imposes 1-2 time, uses 15-20 kilogram/mu at every turn.
3, can do towards fertilising, Sui Shuichong executes, and in crop growth period, 10-15 dashes and executes once, uses 5-10 kilogram/mu at every turn.
4, at continuous cropping disease and the fungus disease by soil infection, make base fertilizer and use, amount of application 20-25 kilogram/mu.
5,, directly spray on plant or fruit after B can being added the dilution of 10 times of water at fungus disease by brone infection.
Product applicable crops of the present invention and diseases prevention scope:
1, use crop: the nutrients structure in the product of the present invention can be realized rational application of fertilizers, i.e. basis The need of different soil properties, nutritional deficiency situation and Different Crop are supported characteristic and are adjusted the nutrient proportioning, can accomplish Cut the waste again in the time of the rational application of fertilizer, so product of the present invention is for various field crops, various vegetables Snake melon orchard crop, various fruit tree kind crop and various types of soil are developed respectively optimal And the serial special product of best results.
2, diseases prevention scope: product Streptomyces of the present invention fermentation metabolite---Polyoxin is A kind of broad spectrum activity antibiotic all has most of pathogenic fungis such as mastigomycetes, sac fungus and Fungi Imperfectis Strong inhibitory action is to vegetables damping-off, rice seedling blight, banded sclerotial blight, early blight of tomato, late blight Disease, gray mold, leaf mold, wheat powdery mildew, flowers powdery mildew, leek powdery mildew, eggplant Huang wither Disease, cucumber fusarium axysporum, the climing Pueraria lobota disease of watermelon, black leg of beet, root rot, graywall, tobacco Rust, black points, black stem, fruit tree putrefaction disease, powdery mildew, cotton seedling blight, ginseng blackspot The fungus diseases such as disease, epidemic disease, tealeaves black spot, control rate reaches more than 70%, wherein, to various The powdery mildew of crop, the control rate of damping-off reach more than the 90-95%.
Claims (1)
1, a kind of industrialized preparing process of economic benefits and social benefits composite microbiological fertilizer is characterized in that:
A. the preparation of colloid bacillus cereus microbial inoculum: earlier colloid bacillus cereus original strain (Bacillusmucilaginosus Krassilnikov Accc10012) is seeded to nitrogen-free agar and activates, 27 ℃-29 ℃ constant temperature culture 3-4 days, after treating that lawn covers with, produce bacterial classification, then this bacterial classification is inoculated in by the part by weight of substratum charging capacity 5% and carries out one grade fermemtation in the first class seed pot and cultivate, the tank body volume is 100 liters, substratum is with improveing Ah Xu shellfish substratum, press after 24 hours to plant to the secondary seed jar and carry out the second order fermentation cultivation, the tank body volume is 1000 liters, substratum is the same, after 24 hours, press to plant again and to the three grade fermemtation jar, carry out three grade fermemtation, the tank body volume is 10000 liters, and substratum starch ammonium, pH value are 7.2-7.4; Stop fermentation after 36 hours, with bacterium liquid with the part by weight whip attachment of weathered coal powder by 1: 5, the colloid bacillus cereus microbial inoculum;
B. the preparation of drappus microbial inoculum: streptomycete original strain (Streptomyces aureusWaksmanet Gurtis CFcc3010) is seeded to slant medium carries out activation culture, 28 ℃-29 ℃ constant temperature culture 5-6 days, after treating that spore covers with, produce bacterial classification; Then this bacterial classification is inoculated in by the part by weight of substratum charging capacity 5% and carries out one grade fermemtation in the first class seed pot, the tank body volume is 100 liters; Press kind to carry out second order fermentation to the secondary seed jar bacterial classification after 48 hours, the tank body volume is 1000 liters; Press after 72 hours to plant and carry out three grade fermemtation to the three grade fermemtation jar, the tank body volume is 10000 liters; Above-mentioned one, two, three general fermention medium that ferments; The visible mycelia of microscopically fracture after 48 hours, the thalline self-dissolving, and detect the tiring when reaching the 1400-1500 potency unit of mycins of supporting more, can stop fermentation; Through Plate Filtration, its filtrate and weathered coal powder be by 1: 6 part by weight with fermented liquid, whip attachment, drappus microbial inoculum;
C. the preparation of economic benefits and social benefits composite microbiological fertilizer, prepare by following weight ratio of constituents example:
Weathered coal 25-30%
Bone meal 8-10%
Monoammonium phosphate 8-10%
Urea 20-24%
Vitriolate of tartar 12-15%
Zinc sulfate 0.3-0.5%
Borax 0.3-0.5%
Sal epsom 0.03-0.05%
Manganous sulfate 0.03-0.05%
Copper sulfate 0.003-0.005%
Ferrous sulfate 0.003-0.005%
Sodium orthomolybdate 0.003-0.005%
Colloid bacillus cereus microbial inoculum 3-5%
Drappus microbial inoculum 3-5%
Wherein colloid bacillus cereus microbial inoculum, drappus microbial inoculum are packed separately respectively, and other composition is hybrid packed.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200410050683 CN1262519C (en) | 2004-10-26 | 2004-10-26 | Industrialization manufacturing method of double effect composite microbial manure |
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| CN 200410050683 CN1262519C (en) | 2004-10-26 | 2004-10-26 | Industrialization manufacturing method of double effect composite microbial manure |
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| CN1600751A CN1600751A (en) | 2005-03-30 |
| CN1262519C true CN1262519C (en) | 2006-07-05 |
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Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1313594C (en) * | 2005-09-19 | 2007-05-02 | 王永年 | Preparation method of epiphyte cellular fluid for dual purpose of edible and medicinal use |
| CN101239847B (en) * | 2008-03-07 | 2011-04-20 | 山东省农业科学院土壤肥料研究所 | Liquid composite microbial fertilizer and its preparation method |
| CN102267838B (en) * | 2010-08-27 | 2013-10-23 | 苏州仁成生物科技有限公司 | Microbial composite fertilizer and preparation method thereof |
| CN102816028B (en) * | 2012-09-10 | 2014-01-29 | 山东金正大生态工程股份有限公司 | Biological disease prevention type greenhouse vegetable planting fertilizer and preparation method and application thereof |
| CN106116717A (en) * | 2016-06-30 | 2016-11-16 | 福建省田伯生物肥有限公司 | A kind of Folium Camelliae sinensis fertilizer and production technology thereof |
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