CN1257683C - Corn green fodder - Google Patents
Corn green fodder Download PDFInfo
- Publication number
- CN1257683C CN1257683C CNB2003101192918A CN200310119291A CN1257683C CN 1257683 C CN1257683 C CN 1257683C CN B2003101192918 A CNB2003101192918 A CN B2003101192918A CN 200310119291 A CN200310119291 A CN 200310119291A CN 1257683 C CN1257683 C CN 1257683C
- Authority
- CN
- China
- Prior art keywords
- pseudomonas
- lactic acid
- bacterium
- feed
- probio
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 235000002017 Zea mays subsp mays Nutrition 0.000 title claims abstract description 22
- 235000005822 corn Nutrition 0.000 title claims abstract description 22
- 235000005824 corn Nutrition 0.000 title claims abstract description 22
- 240000008042 Zea mays Species 0.000 title description 4
- 241000894006 Bacteria Species 0.000 claims abstract description 86
- 241000209149 Zea Species 0.000 claims abstract description 18
- 239000011780 sodium chloride Substances 0.000 claims abstract description 13
- 150000003839 salts Chemical class 0.000 claims abstract description 12
- 235000013312 flour Nutrition 0.000 claims abstract description 11
- 235000013379 molasses Nutrition 0.000 claims abstract description 11
- 241000186660 Lactobacillus Species 0.000 claims abstract description 10
- 229940039696 Lactobacillus Drugs 0.000 claims abstract description 10
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims description 34
- 239000004310 lactic acid Substances 0.000 claims description 34
- 235000014655 lactic acid Nutrition 0.000 claims description 34
- 241000209094 Oryza Species 0.000 claims description 15
- 229910052594 sapphire Inorganic materials 0.000 claims description 15
- 239000010980 sapphire Substances 0.000 claims description 15
- 238000011081 inoculation Methods 0.000 claims description 13
- 239000004460 silage Substances 0.000 claims description 13
- 241000589774 Pseudomonas sp. Species 0.000 claims description 11
- 241000193830 Bacillus <bacterium> Species 0.000 claims description 9
- 238000003860 storage Methods 0.000 claims description 8
- 241000191998 Pediococcus acidilactici Species 0.000 claims description 7
- 238000007796 conventional method Methods 0.000 claims description 5
- 239000012467 final product Substances 0.000 claims description 5
- 238000002360 preparation method Methods 0.000 claims description 2
- 241000589516 Pseudomonas Species 0.000 abstract description 37
- 230000000694 effects Effects 0.000 abstract description 12
- 238000000855 fermentation Methods 0.000 abstract description 11
- 230000004151 fermentation Effects 0.000 abstract description 10
- 238000007599 discharging Methods 0.000 abstract description 9
- 244000144972 livestock Species 0.000 abstract description 8
- 239000002253 acid Substances 0.000 abstract description 4
- 201000010099 disease Diseases 0.000 abstract description 3
- 230000004899 motility Effects 0.000 abstract description 3
- 230000000529 probiotic Effects 0.000 abstract 3
- 239000006041 probiotic Substances 0.000 abstract 3
- 235000018291 probiotics Nutrition 0.000 abstract 3
- 241000602080 Dracaena fragrans Species 0.000 abstract 1
- 230000002860 competitive Effects 0.000 abstract 1
- 241001438449 Silo Species 0.000 description 8
- 241000196324 Embryophyta Species 0.000 description 7
- 239000000654 additive Substances 0.000 description 5
- 230000001580 bacterial Effects 0.000 description 5
- 230000035611 feeding Effects 0.000 description 5
- 240000000218 Cannabis sativa Species 0.000 description 4
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 4
- 239000004459 forage Substances 0.000 description 4
- 239000002054 inoculum Substances 0.000 description 4
- 235000016709 nutrition Nutrition 0.000 description 4
- 230000000996 additive Effects 0.000 description 3
- 244000052616 bacterial pathogens Species 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 244000005700 microbiome Species 0.000 description 3
- 230000035764 nutrition Effects 0.000 description 3
- 230000036284 oxygen consumption Effects 0.000 description 3
- 239000000047 product Substances 0.000 description 3
- WQZGKKKJIJFFOK-GASJEMHNSA-N D-Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 2
- 241000238631 Hexapoda Species 0.000 description 2
- 230000036740 Metabolism Effects 0.000 description 2
- 241000283898 Ovis Species 0.000 description 2
- 235000014633 carbohydrates Nutrition 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 239000008103 glucose Substances 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 230000004060 metabolic process Effects 0.000 description 2
- 230000035786 metabolism Effects 0.000 description 2
- 229910052760 oxygen Inorganic materials 0.000 description 2
- MYMOFIZGZYHOMD-UHFFFAOYSA-N oxygen Chemical compound O=O MYMOFIZGZYHOMD-UHFFFAOYSA-N 0.000 description 2
- 239000001301 oxygen Substances 0.000 description 2
- 238000011105 stabilization Methods 0.000 description 2
- 230000001629 suppression Effects 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 210000000805 Cytoplasm Anatomy 0.000 description 1
- 241000194033 Enterococcus Species 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 210000003495 Flagella Anatomy 0.000 description 1
- 241000726221 Gemma Species 0.000 description 1
- 231100000678 Mycotoxin Toxicity 0.000 description 1
- 210000004681 Ovum Anatomy 0.000 description 1
- 241000194017 Streptococcus Species 0.000 description 1
- 210000004243 Sweat Anatomy 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- 150000001412 amines Chemical class 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 230000003851 biochemical process Effects 0.000 description 1
- 230000037396 body weight Effects 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- CURLTUGMZLYLDI-UHFFFAOYSA-N carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 1
- 239000001569 carbon dioxide Substances 0.000 description 1
- 229910002092 carbon dioxide Inorganic materials 0.000 description 1
- 210000004027 cells Anatomy 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000005520 cutting process Methods 0.000 description 1
- 230000001066 destructive Effects 0.000 description 1
- 235000019621 digestibility Nutrition 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 238000004043 dyeing Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000003203 everyday Effects 0.000 description 1
- 235000021050 feed intake Nutrition 0.000 description 1
- 230000003031 feeding effect Effects 0.000 description 1
- 239000002068 microbial inoculum Substances 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 239000002636 mycotoxin Substances 0.000 description 1
- 230000003000 nontoxic Effects 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000000474 nursing Effects 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 230000000050 nutritive Effects 0.000 description 1
- 150000007524 organic acids Chemical class 0.000 description 1
- 235000019629 palatability Nutrition 0.000 description 1
- 230000001717 pathogenic Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 230000001850 reproductive Effects 0.000 description 1
- -1 salts carbohydrate Chemical class 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 239000010902 straw Substances 0.000 description 1
- 235000019786 weight gain Nutrition 0.000 description 1
Abstract
The present invention relates to corn ensilage feed which is composed of green corn plants, two strains of lactobacillus, probiotic pseudomonas, molasses, corn flour and salt; the probiotic pseudomonas is added into the feed, and the probiotic pseudomonas and the lactobacillus are used in a matching mode; Because the present invention has the characteristics of strong stress resistance, strong motility and strong acid resistance, the present invention has strong competitive power under the aerobic condition, can inhibit the number of aerobic mixed bacterium, and can reduce the feed quality reduction caused by the secondary fermentation of the mixed bacterium in the feed discharging stage; feeding experiments indicate: the feed can promote the growth of livestock, and has obvious effect on the growth and the disease resistance of livestock.
Description
Technical field
The present invention relates to a kind of corn Silage, be applicable to the feed that ox, sheep are fed.
Background technology
Nature, plant surface harmful microorganism and beneficial microbe quantity with than being 10: 1, the biochemical process of silo crop just can lose the nutrition in the silo crop owing to the harmful microbe effect if give free rein in a large number.Manually add the beneficial bacterium bacterial classification and adopt, reach the high quantity of advantage as early as possible as homogeneity type (minute daughter bacteria bacterium sugar can be produced the lactic acid bacteria of molecule lactic acid) lactic acid bacteria through screening, accelerate sweat, produce a large amount of lactic acid rapidly, the pH value is descended, thereby suppress the harmful microbe activity, thereby reach the purpose of preserving nutrition, therefore, use suitable silage additive most important in ensilage.
Ensilage is the feeding product that forage grass and straw are formed through anaerobic fermentation.The process of ensiling is:
Oxyphie: forage grass is put in the silo granary into some biochemical system in (also can be special bag silo or silage tower) plant still in work by chopping.Phytoenzyme is degraded to carbon dioxide and water to soluble sugar, and albumen is converted into amino acid and amine.These reactions can destroy nutritional labelings, and reduce the quantity of useful fermenting enzyme material, and this stage produces too much heat and can cause browning reaction, but this will reduce the digestibility of carbohydrate content in the plant and protein.Therefore, should reduce the time in oxyphie stage as far as possible, carry out the transition to next ensiling stage as early as possible.
Silage fermentation: the fermentation of the acid-producing bacteria of anaerobism roughly will continue for one to two week.The forage grass of moisture 65%～70% is faster than the forage grass fermentation of moisture low (as 50% moisture), and the factors such as mature condition of floristics, cutting mode, results time, plant also can influence the efficient of fermentation in addition.The ensiling type also can have influence on the process of fermentation, and the tower dry loss late of concrete ensiling is less than horizontal silo and open-air horizontal silo.
Stabilization sub stage: fermentative microorganism can utilize the soluble sugar part in the plant gradually and absorb, and ensiling material acidity is reduced to pH3.5～4.2.The biochemical activity of ensiling this moment material reduces even stops, and the ensiling thing has entered the stabilization sub stage.This stage must be an anaerobism.Enter horizontal silo as oxygen from the breakage that seals, will increase the activity of mould, yeast and oxyphie bacterium.These microorganisms can cause the degraded of ensilage, produce mycotoxin and pathogenic bacteria, and the nutriment loss can be very big.
The discharging stage: open horizontal silo, ensilage just is exposed in the oxygen, and ensilage begins degraded.Heat in the ensilage and absorbable nutriment will lose.Temperature raises 10 ℃, and the loss of expose portion dry every day will reach 3%, so should try one's best reduction exposure and open-assembly time.
Additives for ensiling
The U.S., Australian and European, microbionation agent (inoculants) is the most frequently used silage additive, this product can increase beneficial bacterium quantity, replenishes natural lactic acid bacteria in the feeding crop and ferments fast and effectively when helping ensiling.This series products can contain one or more following bacterial classifications usually: Bacillus acidi lactici, class streptococcus and enterococcus, pediococci etc.Wherein should to produce the lactic acid ability with sugar fermentation be by force principle in the selection of Bacillus acidi lactici, therefore, homogeneity type fermentative lactobacillus (homo-fermentative lactic acid bacteria) is one and selects preferably, it can produce 2 molecule lactic acid with a part glucose, and produce L (+) type lactic acid, and the heterogeneous type lactic acid bacteria is used a part glucose production a part lactic acid, and is D (-) the type lactic acid that domestic animal is difficult for metabolism, neither thank to die.Other common additives for ensiling has: organic acid, inorganic salts carbohydrate.
After its advantage of ensilage is that the utilized nutriment that the soil is gathered in the crops maximizes,
Concrete effect is as follows:
Ensilage feed nutrient loss late is dried nutritive loss 30%～50% between 5%～15%.
Ensiling can keep the juice of feed, and hay is moisture 14%～17%, and the ensiling water content can reach 70%, is beneficial to livestock digestion, good palatability.
Ensilage occupied ground space is little, and the density height because of moisture height, is difficult for accidents such as breaking out of fire, storage safety.
The process of ensiling can eliminate destructive insects and germ, and the high acidity of anoxic can not be survived insect and worm's ovum, and this environment adds the competition of beneficial bacterium, and harmful germ is killed or is suppressed, and weeds also are utilized through ensiling, thereby reduces weeds in field quantity.
Summary of the invention
The object of the invention is that the corn Silage of development is by sapphire rice plant, and two strains of lactic acid bacteria Lactic acid bacteria, probio pseudomonas Pseudomonas sp, molasses, corn flour and salt are formed; Its main feature is: added the probio pseudomonas in feed after, pseudomonas and lactic acid bacteria are used, and because of it has strong resistance, motility is strong, and the characteristics that acid resistance is strong have competitiveness strong under the aerobic situation, can suppress the quantity of the assorted bacterium of oxygen consumption; Can reduce reduction in the discharging stage of feed because of assorted microzyme secondary fermentation feed quality; This feed shows through feeding experiment: can promote the livestock growth, to the livestock growth is disease-resistant significant effect be arranged.
Corn Silage of the present invention, this feed is by sapphire rice plant, two strains of lactic acid bacteria Lactic acid bacteria, probio pseudomonas Pseudomonas sp preserving number: the pseudomonad of CGMCC No.0208., molasses, corn flour and salt are formed, and wherein two strains of lactic acid bacteria is Bacillus acidi lactici Lactobacillus and Pediococcus acidilactici Pediococcus; Each set of dispense ratio is: sapphire rice plant 1000 grams, two strains of lactic acid bacteria inoculation bacterium several 2 * 10
8Individual, wherein Bacillus acidi lactici Lactobacillus inoculates bacterium several 1 * 10
8Individual, Pediococcus acidilactici Pediococcus inoculates bacterium several 1 * 10
8Individual, probio pseudomonas Pseudomonas sp inoculates bacterium several 5 * 10
8Individual, molasses 15-30g, corn flour 5-10g, salt 10-30g, 15-30 ℃ of storage in the ensiling container of packing into.
The preparation method of corn Silage with sapphire rice plant, is cut into the 10-30mm size, according to a conventional method two kinds of lactic acid bacterias and probio are inoculated on the sapphire rice plant, add molasses, corn flour and salt again, mix, 15-30 ℃ of storage gets final product in the ensiling container of packing into.
Corn Silage of the present invention adds acting as of probio pseudomonas:
Probio pseudomonas Pseudomonas sp. is a kind of pseudomonas layer, Chinese patent: CN94105175.7, preserving number: CGMCC No.0208.
Probio pseudomonas Pseudomonas sp. bacterial classification has the straight thin rod shape of cell, Gram-positive, no gemma, polar flagella, motion, the uneven characteristic of protoplasm dyeing, reproductive capacity is strong after tested, and fermented and cultured can reach 20,000,000,000/milliliter in 24～36 hours, low temperature resistant, high temperature, salt tolerant alkali, arid have stronger resistivity to adverse circumstance.
The production of probio pseudomonas Pseudomonas sp. bacterial classification has intermittence, grow the existing oxygen consumption stage, anaerobic stages is arranged again, in ensilage, can preserve, can breed and suppress the growth of other good oxa-bacterium fast at aerobic environment, and can under the ensiling condition of low pH value, breed.
Probio pseudomonas Pseudomonas sp. bacterium belongs to the nontoxic utmost point through check.
Probio pseudomonas Pseudomonas sp. additive is through feeding experiment, to the livestock growth is disease-resistant significant positive effect arranged.
Probio pseudomonas Pseudomonas sp. and lactic acid bacteria are used, because of it has strong resistance, motility is strong, the characteristics that acid resistance is strong have competitiveness strong under the aerobic situation, can suppress the quantity of the assorted bacterium of oxygen consumption, reduce reduction in the discharging stage because of assorted microzyme secondary fermentation feed quality, itself is searched for food by livestock, can promote the livestock growth, and resistant effect is arranged.
The contrast test of corn Silage of the present invention:
The variation of pH value
The pH value of different ensiling time samplings
Table 1 adds the pH value of probio pseudomonas
| Fate | 0 | 1 | 2 | 4 | 6 | 8 | 10 | 20 | 40 |
| pH | 5.8 | 5.1 | 4.5 | 4.2 | 4.0 | 3.80 | 3.55 | 3.55 | 3.54 |
Table 2 does not add the identical pH value of other condition of probio pseudomonas
| Fate | 0 | 1 | 2 | 4 | 6 | 8 | 10 | 20 | 40 |
| pH | 5.8 | 5.0 | 4.5 | 4.2 | 3.90 | 3.80 | 3.55 | 3.55 | 3.54 |
Two handle no significant difference, and the probio pseudomonas reduces the not influence of ensilage pH value to the lactic acid bacteria metabolism.
The variation of flora quantity
Table 3 adds probio pseudomonas ensilage flora and changes bacterium number/Kg
| The mushroom sky | Lactic acid bacteria | General bacterium (assorted bacterium) | Yeast | Pseudomonas |
| 1 | 1.5×10 10 | 4.5×10 8 | 4.0×10 4 | 5.0×10 8 |
| 2 | 2.5×10 11 | 2.0×10 8 | 4.0×10 4 | 5.0×10 8 |
| 4 | 4.0×10 12 | 8.0×10 7 | 7.0×10 3 | 4.0×10 8 |
| 6 | 1.7×10 12 | 5.0×10 7 | 2.0×10 2 | 3.0×10 8 |
| 10 | 8.0×10 11 | 2.2×10 6 | 1.0×10 2 | 2.0×10 8 |
| 20 | 9.2×10 10 | 1.0×10 6 | 4.0×10 | 1.3×10 8 |
| 40 | 6.7×10 10 | 8.5×10 5 | 2.0×10 | 9.0×10 7 |
Table 4 does not add probio pseudomonas ensilage flora and changes bacterium number/Kg
| The mushroom sky | Lactic acid bacteria | General bacterium (assorted bacterium) | Yeast |
| 1 | 1.5×10 10 | 4.3×10 8 | 3.5×10 4 |
| 2 | 2.4×10 11 | 2.0×10 8 | 2.0×10 4 |
| 4 | 3.5×10 12 | 7.0×10 7 | 4.0×10 3 |
| 6 | 1.5×10 12 | 4.5×10 7 | 1.5×10 2 |
| 10 | 8.0×10 11 | 2.0×10 6 | 1.0×10 2 |
| 20 | 8.0×10 10 | 1.2×10 6 | 3.0×10 |
| 40 | 6.0×10 10 | 9.0×10 5 | 1.5×10 |
Table 3, table 4 explanation probio pseudomonas does not have negative effect to the propagation of lactic acid bacteria, the probio pseudomonas is in ensilage, quantity reduces the far low and general bacterium of speed (assorted bacterium), in its quantity of later stage far above general bacterium (assorted bacterium).
Flora changed (40 days) bacterium number/Kg after table 5 added the discharging of probio pseudomonas
| Mushroom hour | General bacterium (assorted bacterium) in the inoculation pseudomonas | Do not inoculate the general bacterium of ensilage (assorted bacterium) number of pseudomonas | Pseudomonas number in the inoculation pseudomonas |
| 6 hours | 8.7×10 5 | 9.5×10 5 | 9.5×10 7 |
| 24 hours | 1.0×10 6 | 8.0×10 6 | 2.0×10 8 |
| 48 hours | 1.2×10 6 | 4.0×10 8 | 6.0×10 8 |
| 96 hours | 1.7×10 6 | 8.0×10 8 | 1.5×10 9 |
After discharging, general bacterium (assorted bacterium) propagation of not inoculating in the ensilage of pseudomonas is very fast, and general bacterium (assorted bacterium) is suppressed in the ensilage of inoculation pseudomonas, and pseudomonas propagation is very fast.
The nursing experiment of table 6 sheep
| Handle average | The ensilage of inoculation pseudomonas+lactic acid bacteria | Do not inoculate the ensilage of pseudomonas inoculating lactic acid bacterium | Do not inoculate the ensilage of any microbial inoculum |
| Experiment initial body heavy (Kg) | 8.00 | 7.90 | 8.00 |
| Experiment opisthosoma heavy (Kg) | 9.35 | 9.10 | 9.00 |
| Rate of body weight gain | 135 | 120 | 100 |
Three groups of processing feed intakes are identical as can be seen from the table, and it is best that the ensilage of inoculation pseudomonas+lactic acid bacteria is fed experiment effect.
Conclusion:
The interpolation pseudomonas does not have obvious influence to the changing down of pH value in ensilage.
Adding pseudomonas in ensilage does not have obvious influence to lactic acid bacteria propagation and general bacterium (assorted bacterium) and yeast number change under anaerobic, and quantity is far above general bacterium (bacterium of mixing) during the discharging of pseudomonas quantity.
The general bacterial multiplication of processing that does not add pseudomonas after the discharging is very fast, pseudomonas number propagation is very fast after the processing discharging of interpolation pseudomonas, and wherein general bacterium (assorted bacterium) propagation is slower, pseudomonas obviously suppresses general bacterium (assorted bacterium), bring into play little ecological effect, reduced the loss of nutrition in the feed secondary fermentation.
Add the ensilage of probio pseudomonas, the pseudomonas feeding effect of being searched for food is best.
The specific embodiment
Embodiment 1
Sapphire rice plant 1000 grams are cut into the 10mm size, according to a conventional method with two kinds of lactobacillus inoculum bacterium several 2 * 10
8, wherein Bacillus acidi lactici is inoculated bacterium several 1 * 10
8Individual, Pediococcus acidilactici inoculation bacterium several 1 * 10
8Individual, probio pseudomonas inoculation bacterium several 5 * 10
8Individual being inoculated on the sapphire rice plant adds molasses 15g again, corn flour 5g, and salt 10g mixes, and 15-30 ℃ of storage gets final product in the ensiling container of packing into.
Embodiment 2
Sapphire rice plant 1000 grams are cut into the 20mm size, according to a conventional method with two kinds of lactobacillus inoculum bacterium several 2 * 10
8, wherein Bacillus acidi lactici is inoculated bacterium several 1 * 10
8Individual, Pediococcus acidilactici inoculation bacterium several 1 * 10
8Individual, probio pseudomonas inoculation bacterium several 5 * 10
8Individual being inoculated on the sapphire rice plant adds molasses 20g again, corn flour 7g, and salt 20g mixes, and 15-30 ℃ of storage gets final product in the ensiling container of packing into.
Embodiment 3
Sapphire rice plant 1000 grams are cut into the 30mm size, according to a conventional method with two kinds of lactobacillus inoculum bacterium several 2 * 10
8, wherein Bacillus acidi lactici is inoculated bacterium several 1 * 10
8Individual, Pediococcus acidilactici inoculation bacterium several 1 * 10
8Individual, probio pseudomonas inoculation bacterium several 5 * 10
8Individual being inoculated on the sapphire rice plant adds molasses 30g again, corn flour 10g, and salt 30g mixes, and 15-30 ℃ of storage gets final product in the ensiling container of packing into.
Claims (2)
1, a kind of corn Silage, it is characterized in that this feed is by sapphire rice plant, two strains of lactic acid bacteria Lactic acid bacteria, probio pseudomonas Pseudomonas sp preserving number are that pseudomonad, molasses, corn flour and the salt of CGMCC NO.0208 is formed, and wherein two strains of lactic acid bacteria is Bacillus acidi lactici Lactobacillus and Pediococcus acidilactici Pediococcus; Each set of dispense ratio is: sapphire rice plant 1000 grams, two strains of lactic acid bacteria inoculation bacterium several 2 * 10
8Individual, wherein Bacillus acidi lactici Lactobacillus inoculates bacterium several 1 * 10
8Individual, Pediococcus acidilactici Pediococcus inoculates bacterium several 1 * 10
8Individual, probio pseudomonas Pseudomonas sp inoculates bacterium several 5 * 10
8Individual, molasses 15-30g, corn flour 5-10g, salt 10-30g, 15-30 ℃ of storage in the ensiling container of packing into.
2, the preparation method of corn Silage according to claim 1, it is characterized in that sapphire rice plant, be cut into the 10-30mm size, according to a conventional method two kinds of lactic acid bacterias and probio are inoculated on the sapphire rice plant, add molasses, corn flour and salt again, mix, 15-30 ℃ of storage gets final product in the ensiling container of packing into.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2003101192918A CN1257683C (en) | 2003-12-03 | 2003-12-03 | Corn green fodder |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2003101192918A CN1257683C (en) | 2003-12-03 | 2003-12-03 | Corn green fodder |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1623421A CN1623421A (en) | 2005-06-08 |
| CN1257683C true CN1257683C (en) | 2006-05-31 |
Family
ID=34761326
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB2003101192918A Expired - Fee Related CN1257683C (en) | 2003-12-03 | 2003-12-03 | Corn green fodder |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1257683C (en) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100382718C (en) * | 2005-12-01 | 2008-04-23 | 中国科学院新疆生态与地理研究所 | Corn Silage bacterial agent |
| CN102550889B (en) * | 2012-03-26 | 2013-05-01 | 四川省畜牧科学研究院 | Chicory and ensiled corn mixed ensiling method |
| CN104126741B (en) * | 2014-07-08 | 2016-08-24 | 中国科学院广州能源研究所 | The herbal Silaging method of the energy of a kind of anaerobic fermentation and ensiling material mixing device |
| CN111053156A (en) * | 2019-11-22 | 2020-04-24 | 富民前龙养殖有限公司 | Preparation method of ecological feed |
-
2003
- 2003-12-03 CN CNB2003101192918A patent/CN1257683C/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| CN1623421A (en) | 2005-06-08 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN106173363B (en) | The method for producing cattle and sheep complete feed as raw material staged fermentation using edible fungus bran | |
| CN101558821B (en) | Animal feed and preparation method thereof | |
| CN101449752B (en) | Composite microbial inoculant for corn silage and use method thereo | |
| CN101449744B (en) | Multifunctional biology aquatic feedstuff additive | |
| CN1199578C (en) | Method for producing straw protein fodder by using microbiological straw fermentation | |
| CN1319464C (en) | Industrialized product of plant straw feeding stuff and its preparing method | |
| CN1224333C (en) | Special-purpose additive for ensiling corn | |
| AU2002239449B2 (en) | Mixed cultures for improved fermentation and aerobic stability of silage | |
| CN105002116A (en) | Microorganism straw feed microbial agent and preparation method thereof | |
| CN111838408A (en) | Functional amino acid enzyme and preparation method thereof | |
| CN105918615B (en) | Large-scale production method of rice and wheat straw microbial feed | |
| CN102696860A (en) | Highly efficient and low-cost microbiological feed proteins based on vinegar residue and miscellaneous meal | |
| CN1190145C (en) | Process for producing stalk protein feed by multi-culture united anaerobic fermentation | |
| KR100338122B1 (en) | Process for preparation of fermented feed stuff using byproducts | |
| CN102406068A (en) | Method for preparing microbial fermented feed capable of keeping intestinal health of pigs | |
| CN105146209A (en) | Amaranthus hypochondriacus compound forage wrapping bag as well as preparation method and application thereof | |
| KR20080019333A (en) | Method for producing microbial fermentation litter for livestock using mushroom medium | |
| CN1955276A (en) | Organic microbial composite and use | |
| CN101263864A (en) | Stalk micro storage additive agent | |
| CN105379971A (en) | Method for manufacturing silage corn | |
| CN100382718C (en) | Corn Silage bacterial agent | |
| CN1257683C (en) | Corn green fodder | |
| CN110477190A (en) | A kind of stalk anaerobic fermentation feed and preparation process and formula forage | |
| CN1729828A (en) | A kind of combination of microbiological treating straw | |
| CN102885198A (en) | Breathing bag microbial-storage banana straw feed and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C19 | Lapse of patent right due to non-payment of the annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |