CN1237210A - 植物细胞培养期间改变培养基温度来大量生产紫杉醇的方法 - Google Patents
植物细胞培养期间改变培养基温度来大量生产紫杉醇的方法 Download PDFInfo
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- CN1237210A CN1237210A CN98801232A CN98801232A CN1237210A CN 1237210 A CN1237210 A CN 1237210A CN 98801232 A CN98801232 A CN 98801232A CN 98801232 A CN98801232 A CN 98801232A CN 1237210 A CN1237210 A CN 1237210A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/04—Plant cells or tissues
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
- C12P17/02—Oxygen as only ring hetero atoms
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- Engineering & Computer Science (AREA)
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- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Botany (AREA)
- Cell Biology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Epoxy Compounds (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Description
仪器 | HPLC(Waters,U.S.A) |
柱 | Capcell Pack C18 UG 120(长:250mm,内径:4.6mm) |
柱温 | 40℃ |
流动相 | 乙腈∶水(20%-100%梯度淋洗) |
流速 | 1.0ml/min |
进样体积 | 10μl |
检测器 | UV(227nm),ATTE=3) |
干细胞重(g/L) | 紫杉醇(mg/L) | ||||
21天 | 42天 | 21天 | 42天 | ||
浆果红豆杉 | 24℃下培养 | 7.8 | 10.1 | 0.3 | 0.6 |
24℃->29℃ | 7.3 | 10.4 | 0.3 | 0.7 | |
中国红豆杉Hu-1 | 24℃下培养 | 7.5 | 11.1 | 0.9 | 1.7 |
24℃->29℃ | 7.3 | 9.5 | 0.8 | 2.9 | |
中国红豆杉SYG-1 | 24℃下培养 | 14.3 | 18.1 | 25.1 | 46.7 |
24℃->29℃ | 14.1 | 18.5 | 24.3 | 93.2 | |
东北红豆杉 | 24℃下培养 | 8.5 | 12.4 | 0.5 | 1.1 |
24℃->29℃ | 8.1 | 11.9 | 0.8 | 2.3 | |
Taxus xmedia | 24℃下培养 | 11.6 | 14.2 | 3.4 | 7.6 |
24℃->29℃ | 10.9 | 14.7 | 3.2 | 13.2 |
第21天时改变温度 | 干细胞重(g/L) | |
第21天 | 第42天 | |
24℃ | 14.1 | 18.1 |
从24℃至29℃ | 14.2 | 19.2 |
从24℃至32℃ | 14.2 | 17.9 |
从24℃至20℃ | 14.5 | 15.8 |
温度改变的时间点 | 所产生的紫杉醇(mg/L) | |
第21天 | 第49天 | |
24℃下49天 | 26.0 | 28.8 |
29℃下49天 | 17.8 | 76.5 |
24℃下7天,从第8天为29℃ | 32.2 | 74.9 |
24℃下14天,从第15天为29℃ | 45.8 | 110.3 |
24℃下21天,从第22天为29℃ | 26.7 | 137.5 |
测试组大 | 1 | 2 | 3 | 4 |
第14天 | 9.36 | 9.00 | 11.19 | 11.23 |
第28天 | 15.12 | 14.68 | 15.94 | 17.49 |
第42天 | 17.03 | 18.34 | 15.03 | 16.42 |
测试组天 | 1 | 2 | 3 | 4 |
第14天 | 1.54mg/L | 1.30mg/L | 8.7mg/L | 8.0mg/L |
0.16mg/g | 0.14mg/g | 0.78mg/g | 3.01mg/g | |
第28天 | 23.05mg/L | 30.65mg/L | 39.87mg/L | 47.57mg/L |
1.52mg/g | 2.09mg/g | 2.50mg/g | 2.72mg/g | |
第42天 | 32.90mg/L | 48.35mg/L | 35.87mg/L | 51.83mg/L |
1.93mg/g | 2.65mg/g | 2.39mg/g | 3.16mg/g |
测试组天 | 1 | 2 | 3 | 4 |
第14天 | 9.36 | 6.34 | 9.63 | 9.65 |
第28天 | 15.12 | 15.88 | 12.75 | 14.22 |
第42天 | 17.03 | 11.24 | 12.37 | 15.74 |
测试组天 | 1 | 2 | 3 | 4 |
第14天 | 1.54mg/L | 1.95mg/L | 13.57mg/L | 16.40mg/L |
0.16mg/g | 0.31mg/g | 1.41mg/g | 1.70mg/g | |
第28天 | 23.05mg/L | 48.80mg/L | 48.13mg/L | 79.40mg/L |
1.52mg/g | 3.07mg/g | 3.77mg/g | 5.58mg/g | |
第42天 | 32.90mg/L | 42.20mg/L | 33.20mg/L | 78.23mg/L |
1.93mg/g | 3.75mg/g | 2.68mg/g | 4.97mg/g |
Claims (5)
Applications Claiming Priority (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
KR19970027746 | 1997-06-26 | ||
KR1019980009384A KR100266448B1 (ko) | 1997-06-26 | 1998-03-12 | 식물세포 배양 중의 온도변화에 의한 택솔의 대량생산 방법 |
KR1997/27746 | 1998-03-12 | ||
KR1998/9384 | 1998-03-12 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN1237210A true CN1237210A (zh) | 1999-12-01 |
CN1082546C CN1082546C (zh) | 2002-04-10 |
Family
ID=26632875
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN988012324A Expired - Lifetime CN1082546C (zh) | 1997-06-26 | 1998-06-25 | 植物细胞培养期间改变培养基温度来大量生产紫杉醇的方法 |
Country Status (9)
Country | Link |
---|---|
US (1) | US6589765B1 (zh) |
EP (1) | EP0966540B1 (zh) |
JP (1) | JP3169968B2 (zh) |
KR (1) | KR100266448B1 (zh) |
CN (1) | CN1082546C (zh) |
AU (1) | AU715119B2 (zh) |
CA (1) | CA2264270C (zh) |
DE (1) | DE69819873T2 (zh) |
WO (1) | WO1999000513A1 (zh) |
Families Citing this family (31)
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US8791081B2 (en) * | 1999-08-13 | 2014-07-29 | Case Western Reserve University | MGMT inhibitor combination for the treatment of neoplastic disorders |
ATE399063T1 (de) | 2000-03-20 | 2008-07-15 | Solipat Ag | Vorrichtung und verfahren zum auftragen von beschichtungsmaterial |
IL136232A0 (en) * | 2000-05-18 | 2001-05-20 | Bar Ilan University Res Author | Measurements of enzymatic activity in a single, individual cell in population |
US20050014201A1 (en) * | 2001-10-25 | 2005-01-20 | Mordechai Deuthsch | Interactive transparent individual cells biochip processor |
IL154677A0 (en) * | 2003-02-27 | 2003-09-17 | Univ Bar Ilan | A method and apparatus for manipulating an individual cell |
US9200245B2 (en) | 2003-06-26 | 2015-12-01 | Seng Enterprises Ltd. | Multiwell plate |
US7888110B2 (en) * | 2003-06-26 | 2011-02-15 | Seng Enterprises Ltd. | Pico liter well holding device and method of making the same |
US8597597B2 (en) * | 2003-06-26 | 2013-12-03 | Seng Enterprises Ltd. | Picoliter well holding device and method of making the same |
EP1654347B1 (en) * | 2003-06-26 | 2014-06-04 | Seng Enterprises Limited | Improved materials for constructing cell-chips, cell-chip covers, cell-chip coats, processed cell-chips and uses thereof |
WO2005008626A1 (en) * | 2003-07-11 | 2005-01-27 | University Of North Carolina At Chapel Hill | Methods and systems for controlling a computer using a video image and for combining the video image with a computer desktop |
US20050064524A1 (en) * | 2003-08-11 | 2005-03-24 | Mordechai Deutsch | Population of cells utilizable for substance detection and methods and devices using same |
KR100577094B1 (ko) * | 2003-12-31 | 2006-05-08 | 주식회사 삼양제넥스 | 식물 세포 배양에서 알카노산 또는 이의 염을 이용한 이차대사산물의 대량 생산방법 |
CA2554927A1 (en) * | 2004-02-03 | 2005-08-18 | Chemagis Ltd. | Stable amorphous forms of montelukast sodium |
US7403647B2 (en) * | 2004-09-13 | 2008-07-22 | Seng Enterprises Ltd. | Method for identifying an image of a well in an image of a well-bearing component |
EP1763665A1 (en) * | 2004-07-07 | 2007-03-21 | Seng Enterprises Limited | Method and device for identifying an image of a well in an image of a well-bearing component |
EP1781404A2 (en) * | 2004-08-25 | 2007-05-09 | Seng Enterprises Limited | Method and device for isolating cells |
WO2006080000A1 (en) | 2005-01-25 | 2006-08-03 | Seng Enterprises Ltd. | Device for the studying individual cells |
KR100797534B1 (ko) | 2005-06-03 | 2008-01-24 | 주식회사 삼양제넥스 | 식물세포배양배지에 혼합당 처리를 통한 이차 대사산물의대량 생산방법 |
EP1904638A2 (en) | 2005-07-18 | 2008-04-02 | Metabogal Ltd. | Mucosal or enteral administration of biologically active macromolecules |
US20070141555A1 (en) * | 2005-10-11 | 2007-06-21 | Mordechai Deutsch | Current damper for the study of cells |
US8288120B2 (en) * | 2005-11-03 | 2012-10-16 | Seng Enterprises Ltd. | Method for studying floating, living cells |
US20060223999A1 (en) * | 2006-05-10 | 2006-10-05 | Chemagis Ltd. | Process for preparing montelukast and precursors thereof |
US8392632B2 (en) | 2007-02-14 | 2013-03-05 | Samsung Electronics Co., Ltd | Method and apparatus for data processing in mobile communication system |
US9145540B1 (en) | 2007-11-15 | 2015-09-29 | Seng Enterprises Ltd. | Device for the study of living cells |
EP2237887A2 (en) | 2007-12-26 | 2010-10-13 | Seng Enterprises Ltd. | Device for the study of living cells |
KR20120059451A (ko) | 2009-04-03 | 2012-06-08 | 다이아나플랜트사이언스, 아이엔씨. | 식물 세포 배양 유래 프로시아니딘의 생산 및 추출 |
KR20140031835A (ko) | 2010-10-04 | 2014-03-13 | 다이아나플랜트사이언시즈, 에스.에이.에스. | 식물 세포 배양으로부터 프로시아니딘의 생산 및 추출 |
RU2014137404A (ru) | 2012-02-19 | 2016-04-10 | Проталикс Лтд. | Пероральные единичные лекарственные формы и их применение для лечения болезни гоше |
US20170101633A1 (en) | 2014-02-10 | 2017-04-13 | Protalix Ltd. | Method of maintaining disease stability in a subject having gaucher's disease |
KR101670632B1 (ko) * | 2016-04-26 | 2016-11-09 | 김원배 | 휴대용 변기 |
US11299700B1 (en) | 2021-02-19 | 2022-04-12 | Acequia Biotechnology, Llc | Bioreactor containers and methods of growing hairy roots using the same |
Family Cites Families (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5019504A (en) * | 1989-03-23 | 1991-05-28 | The United States Of America As Represented By The Secretary Of Agriculture | Production of taxol or taxol-like compounds in cell culture |
US5407816A (en) * | 1992-02-20 | 1995-04-18 | Phyton Catalytic, Inc. | Enhanced production of taxol and taxanes by cell cultures of taxus species |
DE4238770C2 (de) * | 1992-03-11 | 1994-01-27 | Buertex Buerker & Co Gmbh | Verrottbare faserverstärkte Folie |
EP1063300B1 (en) * | 1993-11-15 | 2003-09-03 | Mitsui Chemicals, Inc. | A method of producing a taxane-type diterpene and method of obtaining cultured cells which produce the taxane-type diterpene at a high rate |
US5654448A (en) * | 1995-10-02 | 1997-08-05 | Xechem International, Inc. | Isolation and purification of paclitaxel from organic matter containing paclitaxel, cephalomannine and other related taxanes |
-
1998
- 1998-03-12 KR KR1019980009384A patent/KR100266448B1/ko not_active IP Right Cessation
- 1998-06-25 DE DE69819873T patent/DE69819873T2/de not_active Expired - Lifetime
- 1998-06-25 CN CN988012324A patent/CN1082546C/zh not_active Expired - Lifetime
- 1998-06-25 EP EP98929893A patent/EP0966540B1/en not_active Expired - Lifetime
- 1998-06-25 CA CA002264270A patent/CA2264270C/en not_active Expired - Lifetime
- 1998-06-25 AU AU79403/98A patent/AU715119B2/en not_active Expired
- 1998-06-25 WO PCT/KR1998/000179 patent/WO1999000513A1/en active IP Right Grant
- 1998-06-25 JP JP50546299A patent/JP3169968B2/ja not_active Expired - Lifetime
- 1998-06-25 US US09/269,154 patent/US6589765B1/en not_active Expired - Lifetime
Also Published As
Publication number | Publication date |
---|---|
JP3169968B2 (ja) | 2001-05-28 |
CA2264270A1 (en) | 1999-01-07 |
CA2264270C (en) | 2002-08-27 |
CN1082546C (zh) | 2002-04-10 |
AU7940398A (en) | 1999-01-19 |
EP0966540B1 (en) | 2003-11-19 |
WO1999000513A1 (en) | 1999-01-07 |
US6589765B1 (en) | 2003-07-08 |
EP0966540A1 (en) | 1999-12-29 |
DE69819873T2 (de) | 2004-09-02 |
AU715119B2 (en) | 2000-01-20 |
DE69819873D1 (de) | 2003-12-24 |
KR100266448B1 (ko) | 2000-09-15 |
JP2000501297A (ja) | 2000-02-08 |
KR19990006371A (ko) | 1999-01-25 |
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Owner name: SAMYANG GENEX BIO CO., LTD. Free format text: FORMER OWNER: SAMYANG GENEX CORP. Effective date: 20130531 |
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Effective date of registration: 20130531 Address after: Seoul, South Korea Patentee after: Nicks, Ji biological Miyaki Address before: Seoul, South Kerean Patentee before: Samyang Genex Corp. |
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Owner name: SAMYANG BIOPHARMACEUTICALS CORPORATION Free format text: FORMER OWNER: SAMYANG GENEX BIO CO., LTD. Effective date: 20140312 |
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