CN1227727A - Multifunctional nutritive liquid and its producing method - Google Patents
Multifunctional nutritive liquid and its producing method Download PDFInfo
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Abstract
A multifunctional nutritive liquid for increasing memorizing power, promoting growth and development and improving immunity is prepared from Chinese-medicinal materials including pilose asiabell root, wolfberry fruit, mulberry fruit and jujube, animal tissue including beaf, animal's spleen and animal's brain, zymocytes including Bifidobacterium, lactobacillus and saccharomycetes, soybean bud, trypsinase, Na2CO3, trace elements compound and flavouring through aqueous extraction of Chinese-medicinal materials, enzymolyzing animal tissue, protein transform of soybean bud, fermentation and mixing.
Description
The present invention relates to nutrient solution, refer in particular to a kind of multifunctional nutrient liquid and manufacture method thereof.
Along with improving constantly of development of producing and living standard, people's life progressively moves towards fairly well-off from simply having adequate food and clothing, for improving growing quality of life, various nutritional supplements arise at the historic moment, nutrient solution wherein because of its taking convenience, be absorbed by the body easily, instant effect, and extremely people's favor, though the kind of nutrient solution is a lot, but the multifunctional nutrient liquid that mostly based on the composition that has additional nutrients, can improve memory simultaneously, promote to grow and improve immunoloregulation function is not really seen appearance.
The object of the present invention is to provide a kind of multifunctional nutrient liquid and manufacture method thereof that improvement is remembered, promotion is grown and improve immunoloregulation function that have, for nutritional supplement increases new varieties, reach and build up health, promote to grow, reduce the purpose of disease, raising anti pathologic immunity ability.
The object of the present invention is achieved like this: a kind of multifunctional nutrient liquid, it is characterized in that this multifunctional nutrient liquid comprises the aqueous extract 5-15 of (parts by weight) Chinese medicine composition, the pancreatin enzymolysis liquid 13-23 of animal tissue, the albumen conversion fluid 50-80 of soybean germ and the mixed liquor after the bacterium fermentation by fermentation thereof, also contain trace compound and flavor enhancement, this Chinese medicine composition comprises: Radix Codonopsis 40-60, matrimony vine 5-15, mulberry fruit 5-15, date 10-30, this animal tissue comprises: beef 20-30, animal spleen 35-45, animal brain 8-18, this zymophyte comprises: Bifidobacterium 0.1-5, Lactobacillus delbrueckii 0.1-5, saccharomycete 0.01-0.5, soybean germ is 200-400, pancreatin 0.1-10, Na
2CO
30.03-1, minor metallic element compound 0.001-0.1, flavor enhancement 4-50.This minor metallic element compound comprises zinc sulfate; This flavor enhancement comprises sugar, honey; This selects for use the Chinese medicine composition in the raw material to comprise Radix Codonopsis 50 grams, matrimony vine 10 grams, mulberry fruit 10 grams, date 20 grams, animal tissue comprises: beef 26 grams, pig spleen 39 grams, pig brain 13 grams, zymophyte comprise: Bifidobacterium 5 grams, Lactobacillus delbrueckii 5 grams, saccharomycete 02 grams, soybean germ 300 grams, Na
2CO
30.8 gram, pancreatin 5 grams, zinc sulfate 0.04 gram, glucose 15 grams, sucrose 30 grams; The material proportion of this nutrient solution is aqueous extract 10 grams, pancreatin enzymolysis liquid 18 grams of animal tissue, albumen conversion fluid 67.2 grams of soybean germ, Bifidobacterium 0.5 gram, Lactobacillus delbrueckii 0.5 gram, Yeast diffusion juice 0.3 gram, zinc sulfate 0.00367 gram, glucose 1.5 grams, sucrose 3 grams of Chinese medicine composition; This manufacture method comprises the steps:
(1) select materials: the raw material of selecting to make multifunctional nutrient liquid comprises (parts by weight): Chinese medicine composition: Radix Codonopsis 40-60, matrimony vine 5-15, mulberry fruit 5-15, date 10-30, the beef 20-30 of animal tissue, animal spleen 35-45, animal brain 8-18, zymophyte: Bifidobacterium 0.1-5, Lactobacillus delbrueckii 0.1-5, saccharomycete 0.01-0.5, soybean germ 200-400, pancreatin 0.1-10, Na
2CO
30.03-1, minor metallic element compound 0.001-0.1, flavor enhancement 4-50;
(2) aqueous extract of preparation Chinese medicine composition:
Above-mentioned Chinese medicine composition was steeped 30-60 minute with the water logging of 4-10 times of weight, the temperature fire was fried in shallow oil 60-90 minute, filtered, and collected aqueous extract for the first time, the water that adds 4-10 times of weight in the medicine quarrel, the temperature fire was fried in shallow oil 60-90 minute, filtered, and collected aqueous extract for the second time, merge aqueous extract twice, be concentrated into the weight of Chinese medicine mixture, make the Chinese medicine aqueous extract, standby;
(3) the pancreatin enzymolysis liquid of preparation animal tissue:
Above-mentioned animal tissue is rubbed with meat grinder, in the 1-4 of animal tissue's weight water doubly, add Na
2CO
3, make its dissolving, add the animal tissue of rubbing, be heated to 90-100 ℃, kept 45-60 minute, be cooled to 40 ℃ subsequently, add pancreatin, enzymolysis 8-10 hour, filter, make the pancreatin enzymolysis liquid of animal tissue, standby;
(4) the albumen conversion fluid of preparation soybean germ:
Get the soybean germ of the long 2-4cm of bud, add water 2-4 doubly, under 100-110 ℃ of condition boiling 30-45 minute, filter, make the albumen conversion fluid of soybean germ, standby;
(5) make multifunctional nutrient liquid:
The albumen conversion fluid of the pancreatin enzymolysis liquid of the aqueous extract of the Chinese medicine composition of above-mentioned (two), (three), (four) preparation, animal tissue and soybean germ merged mix, sterilization treatment under 121 ℃ of conditions is cooled to 40 ℃, adds zymophyte: Bifidobacterium, Lactobacillus delbrueckii and saccharomycete, keep 39 ± 1 ℃, fermented in 15-20 hour, and be warming up to 60 ℃ subsequently, stablized 3 hours, stop fermentation, add trace compound and flavor enhancement, product is made in bottling routinely.
Major advantage of the present invention is:
1, the present invention will have aqueous extract, the animal tissue of the Chinese medicine composition of nourishing function pancreatin enzymolysis liquid, soybean germ the albumen conversion fluid and carry out the fermentation mixed liquor of fermentation process through Bifidobacterium Bifidum, Lactobacillus delbrueckii and yeast, make have body-building, the nutrient solution of multiple function such as health care and anti pathologic immunity adjusting, this nutrient solution utilizes microorganism fungus kind to regulate the interior microecological balance of human body, contain simultaneously the nourishing composition again, have and build up health, the function of improving memory and promoting to grow.
2, the nutrient solution of the present invention feedstock production of pure natural, raw material is cheap, and does not contain any chemical synthesis product, and the health care tonic for pure natural the experiment proved that, does not have any poison and pays effect.
3, manufacture method of the present invention is simple, does not need special equipment, is fit to the preparation of large-scale production or small lot.
The present invention is further described below in conjunction with manufacturing process flow diagram and embodiment.
Fig. 1 is a manufacturing process flow diagram of the present invention;
Embodiment 1:
Multifunctional nutrient liquid of the present invention comprises the aqueous extract of the Chinese medicine composition with nourishing function, the pancreatin enzymolysis liquid of animal tissue, the albumen conversion fluid of soybean germ and through the fermentation mixed liquor of zymophyte fermentation process, cooperate trace compound and flavor enhancement again, Chinese medicine composition wherein comprises: Radix Codonopsis 50 grams, matrimony vine 10 grams, mulberry fruit 10 grams, date 20 grams, animal tissue comprises: beef 26 grams, pig spleen 39 grams, pig brain 13 grams, zymophyte comprises: Bifidobacterium 5 grams, Lactobacillus delbrueckii 5 grams, saccharomycete 0.2 gram, (perhaps with commercially available Yeast diffusion juice 3 grams), soybean germ 300 grams, Na
2CO
30.8 gram, pancreatin 5 grams, zinc sulfate 0.04 gram, white sugar 45 grams.
Manufacture method of the present invention is consulted Fig. 1, and its technological process comprises the steps:
(1) select materials:
Select materials by above-mentioned raw material.
(2) aqueous extract of preparation Chinese medicine composition:
Above-mentioned Chinese medicine composition 90 grams are added water 600 grams to be soaked 45 minutes, the temperature fire was fried in shallow oil 70 minutes, filter, collect aqueous extract for the first time, add the warm fire of water 400 grams in the medicine quarrel and fried in shallow oil 65 minutes, filter and collect aqueous extract for the second time, merge aqueous extract twice, be concentrated into 100 grams, make the aqueous extract of Chinese medicine composition, standby;
(3) the pancreatin enzymolysis liquid of preparation animal tissue:
Above-mentioned animal tissue 78 is restrained with the meat grinder rubbing totally, in 190 gram water, dissolve Na
2CO
30.8 gram adds the animal tissue of rubbing, and is heated to 80 ℃, keeps 50 minutes, is cooled to 40 ℃ subsequently, adds pancreatin and carries out enzymolysis 9 hours, filters, and makes enzymolysis liquid 180 grams of animal tissue.This alkali condition and 40 ℃ of temperature are that pancreatin keeps active optimum condition, nutritional labeling in the animal tissue can be resolved into the amino acid that is absorbed by the body easily effectively, and do not destroy nutritional activities composition wherein, preserve wherein antibody and active ingredient in good condition, can strengthen the anti pathologic immunity regulatory function of human body.
(4) the albumen conversion fluid of preparation soybean germ:
Get soybean germ 300 grams of the long 3cm of bud, add water 700 grams, under 100 ℃ of conditions, steamed 30 minutes, make nutrition, antibody composition and protein dissolution in the soybean germ in water, albumen conversion fluid 672 grams of soybean germ are made in filtration, contain abundant multivitamin, amino acid and active antibodies in this conversion fluid.
(5) make multifunctional nutrient liquid:
The albumen conversion fluid of the pancreatin enzymolysis liquid of the aqueous extract of the Chinese medicine composition of above-mentioned (two), (three), (four) preparation, animal tissue and soybean germ merged mix, sterilization treatment under 121 ℃ of conditions, be cooled to 40 ℃, add Bifidobacterium 5 grams, Lactobacillus delbrueckii 5 grams, saccharomycete 0.2 gram, carry out fermentation process, keep 39 ± 1 ℃, 18 hours, be warming up to 60 ℃ subsequently, stablize and made the fermentation termination in 3 hours, adding zinc sulfate 0.04 gram, white sugar 45 grams, bottling after the assay was approved is packaged into product routinely.Can breed growth rapidly in probiotics Bifidobacterium, Lactobacillus delbrueckii and the saccharomycete nutrient solution after above-mentioned sterilization treatment that adds, therefore contain abundant beneficial bacterium and antibody in this nutrient solution, health is had the memory of improvement, promote to grow and immunoloregulation function.
When the actual production of multifunctional nutrient liquid of the present invention, the pancreatin enzymolysis liquid of the normal aqueous extract animal tissue for preparing earlier Chinese medicine composition respectively and the albumen conversion fluid of soybean germ, after the proportioning mixing, add the zymophyte fermentation, add required trace compound and sugar again.For example the aqueous extract 10 of Chinese medicine composition restrains, the pancreatin enzymolysis liquid 18 of animal tissue restrains, the albumen conversion fluid 67.2 of soybean germ restrains, Bifidobacterium 0.5 restrains, Lactobacillus delbrueckii 0.5 restrains, Yeast diffusion juice 0.3 restrains, zinc sulfate 0.00367 restrains, glucose 1.5 restrains, sucrose 3 restrains.
Embodiment 2:
Multifunctional and nutritional liquid product of the present invention detects through Shandong Prov. Sanitation and Antiepidemic Station, prove that its harmful bacterium is lower than the required standard of medicine food, and contain nutritions such as abundant sugar, amino acid, zinc, test result sees Table 1 and table 2, (wherein selecting for use three batches of products to do parallel test).The detection summary table of three batches of products of table 1
Amino acid content in three batches of products of table 2
Embodiment 3:
| Test item | Batch number | ||
| ?????Ⅰ | ?????Ⅱ | ?????Ⅲ | |
| Plumbous (mg/l) arsenic (mg/l) total reducing sugar (m/l) total amino acid content (g/l) total solid (%) Zn (mg/l) of total plate count (cfu/ml) coliform (cfu/100ml) Salmonella Shigella staphylococcus aureus hemolytic streptococcus mould (cfu/ml) yeast (cfu/ml) | ＜1＜3 do not detect＜1＜1＜0.05＜0.3 75.5 7.41 10.95 14.20 | ＜1＜3 do not detect＜1＜1＜0.05＜0.3 71.5 7.34 10.81 14.80 | ＜1＜3 do not detect＜1＜1＜0.05＜0.3 75.7 7.27 10.94 15.99 |
| Amino acid content (mg/l) | Batch number | ||
| ?????Ⅰ | ?????Ⅱ | ?????Ⅲ | |
| L-aminobutanedioic acid threonine serine glutamic acid proline glycine alanine valine methionine isoleucine leucine tyrosine phenylalanine arginine lysine histidine | ????1890.9 ?????387.1 ?????462.4 ????2586.8 ?????124.9 ?????515.5 ?????807.6 ?????462.9 ?????202.5 ?????304.6 ?????639.2 ??????60.0 ?????580.6 ?????580.6 ?????368.0 ?????161.8 | ????1891.9 ?????390.4 ?????455.9 ????2678.2 ?????119.2 ?????511.7 ?????817.8 ?????458.7 ?????201.4 ?????305.2 ?????629.7 ??????59.5 ?????580.6 ?????580.6 ?????359.1 ?????171.7 | ????1888.8 ?????386.3 ?????450.9 ????2447.1 ?????114.2 ?????510.9 ?????810.4 ?????442.8 ?????199.4 ?????298.9 ?????620.9 ??????59.9 ?????579.9 ?????579.9 ?????370.3 ?????160.5 |
Animal experiment and human feeding trial that multifunctional nutrient liquid of the present invention is done through " Chinese Academy Of Preventive Medicine Research Institute Of Nutrition And Food Hygiene ", criterion according to " the health food function assessment assessment process and the method for inspection ", prove that multifunctional nutrient liquid of the present invention has the memory of improvement, promote to grow and immunoregulation effect.Concrete test and determination methods are as follows:
(1) improve the test of memory effect:
Animal experiment:
Select 15 every group of Kunming kind ablactation small white mouses for use, divide four groups: high dose group, middle dosage group, low dose group and normal control group, irritate stomach every day and be respectively (ml/kg) 15.0,7.5,1.5 and distilled water to nutrient solution, long run test 30 days, animal training carries out diving tower and water maze test, the test of disappearing after the week.
Tester: water maze automatic recording instrument and diving tower instrument.
Data are handled: the water maze laboratory data, and the time of reaching home is used variance analysis, and mistake sum and the number of animals of reaching home are checked with X2; Use variance analysis in diving tower real data, incubation period, mistake sum X
2Check.Experimental result sees Table 3 and table 4.Table 3 multifunctional nutrient liquid is to the influence (step down test) of mouse passive avoidance ability
| Group | Number of animals | Project | Number of animals is shocked by electricity | Incubation period (second) | The mistake sum |
| Dosage group high dose group in the control group low dose group | ???11 ???11 ???11 ???11 | Test after 1 week of test behind the test training achievement 24h after 1 week of test behind the test training achievement 24h after 1 week of test behind the test training achievement 24h after 1 week of test behind the training achievement 24h | ??????10 ???????7 ???????6 ??????10 ???????4 ???????4 ??????10 ???????2 ???????3 ??????10 ???????2 ???????1 | ???94.7±72.3 ???120.5±74.7 ???145.4±59.4 ???144.0±57.3 ???155.5±55.5 ???153.5±50.9 ???166.6±29.8 ???168.6±37.9 | ????22 ????11 ?????8 ????27 ?????5 ?????5 ????23 ?????3 ?????3 ????24 ?????3 ?????1 |
As seen from Table 3:
1, the electric shock number of animals of high, medium and low dosage group and wrong sum all are lower than the normal control group, and incubation period is all longer.
2, high dose group has significant difference on the statistics, (P＜0.05) than normal control group.
Table 4 multifunctional and nutritional is to the influence of mouse water maze test
| Group | Number of animals | Project | Arrive number of animals | Arriving south takes time (second) | The mistake sum |
| Dosage group high dose group in the control group low dose group | ???11 ???11 ???11 ???11 | Test after 1 week of test behind the test training achievement 24h after 1 week of test behind the test training achievement 24h after 1 week of test behind the test training achievement 24h after 1 week of test behind the training achievement 24h | ????10 ????10 ????11 ?????9 ????11 ????11 ?????9 ????11 ????11 ?????9 ????11 ????11 | ??84.4±21.5 ??75.0±28.2 ??73.6±26.9 ??81.4±20.5 ??52.5±24.7 ??60.5±31.1 ??81.6±24.2 ??48.6±19.3 ??49.8±24.7 ??71.4±29.3 ??35.3±11.3 ??46.6±21.6 | ????135 ????109 ????117 ????113 ?????88 ?????80 ????112 ?????67 ?????75 ????114 ?????62 ?????64 |
With table 4 as seen:
1, training test result arrival number of animals difference is little.
2, after 24 hours and the achievement that disappears of 1 week back test, reach home required time and wrong sum, each dosage group is lower than the normal control group, and the significant difference (P＜0.05) on the statistics is all arranged.
(2) human feeding trial:
Select 80 of healthy junior two students, be divided into two groups, experimental group and control group, everyone every day three oral multifunctional nutrient liquid 20ml, the oral equivalent brown sugar water of control group (same package) serve on 30 days, when beginning and end, use " Clinical Memory scale A and table B " to estimate experimenter's memory level respectively, five tests of scale are: associative learning, sensing memory, image are freely remembered, random shape is re-recognized, the contact of portrait characteristics is recalled.Experimental data is checked with seven.Experimental result sees Table 5,
Table 6, table 7 and table 8.
The student remembers the comparison (M ± SD) of achievement before table 5 experiment
| Group | The example number | Association | Point to | Random shape | Image | Portrait |
| The control group experimental group | ???30 ???34 | ??19.12±4.06 ??17.51±4.61 | ??17.83±2.07 ??17.70±2.41 | ??5.57±3.08 ??6.62±3.75 | ???19.87±2.82 ???20.24±3.13 | ???16.67±3.99 ???15.18±3.82 |
Table 6 multifunctional nutrient liquid is remembered the influence (M ± SD) of achievement to the student
Compare P＜0.05 with control group
| Group | The example number | Association | Point to | Random shape | Image | Portrait |
| The control group experimental group | ???30 ???34 | ???21.05±3.37 ???21.10±4.08 | ??18.17±2.20 ??18.47±2.74 | ??4.60±2.65 ??5.20±2.53 | ???21.27±2.99 ???21.09±4.05 | ??15.30±4.29 ??17.50±3.63 |
Multifunctional nutrient liquid the results are shown in Table 7 to the influence of student's memory quotient.Record two groups memory quotient no significant difference before the experiment; When experiment finished, the memory quotient of experimental group obviously improved, and experimental group memory quotient value added highly significant is higher than control group (P＜0.01) table 7 multifunctional nutrient liquid to the influence of student's memory quotient (M ± SD)
Compare P＜0.05, P＜0.01 with control group
| Group | The example number | Memory quotient before the experiment | Experiment back memory quotient | The memory quotient value added |
| The control group experimental group | ???30 ???34 | ????96.7±8.8 ????94.9±10.6 | ????99.5±8.2 ???102.2±12.8 | ????2.8±4.5 ????7.3±8.5 |
Multifunctional nutrient liquid the results are shown in Table 8 to the influence of the total scale score of student.Record total scale score no significant difference of two groups before the experiment; When experiment finished, total scale of experimental group seemed clearly to improvement, and total scale score value added highly significant of experimental group is higher than control group (P＜0.01) table 8 multifunctional nutrient liquid to the influence of the total scale score of student (M ± SD)
Compare P＜0.05, P＜0.01 with control group
| Group | The example number | Score before the experiment | Experiment back score | The memory quotient value added |
| The control group experimental group | ???45 ???44 | ??111.1±10.4 ??109.1±12.4 | ????114.4±9.5 ????117.6±15.0 | ????3.3±5.2 ????8.5±9.9 |
Prove by above-mentioned animal experiment and human feeding trial: multifunctional nutrient liquid of the present invention has the memory impairments of improvement.
(2) animal experiment of the promotion effect of growing:
Do animal experiment with two monthly age kunming mices.Be divided into four groups, high, medium and low dosage group and normal control group, the multifunctional nutrient liquid consumption is respectively 30,10,3 (ml/kg), running water, irritate stomach every day once, female mouse is from becoming pregnant to the continuous clothes of giving of newborn mouse ablactation (21 days), newborn mouse is given continuously clothes 40 days from the back of weaning, and the young situation of test tire, body weight and food utilization, physiological development index the results are shown in Figure 9, table 10, table 11.
Table 9 multifunctional nutrient liquid is to the influence of the young situation of the tire (n=8 of means ± sd)
| Group | Tire number alive | Female-male proportion | The stillborn foetus number | The young sum of childbirth tire (/ only) |
| Dosage group high dose group in the control group low dose group | ?7.8±1.5 ?6.3±2.8 ?7.3±4.2 ?7.8±4.2 | ????1∶1.13 ????1∶1.71 ????1∶2.14 ????1∶0.88 | ????0 ????0 ????0 ????0 | ??????7.8±1.5 ??????6.3±2.8 ??????7.3±4.2 ??????7.8±4.2 |
Table 10 multifunctional nutrient liquid is to the influence of back mouse body weight and the food utilization of the weaning (n=8 of means ± sd)
5 annotate: the influence that data statistics adopting factor variance analysis method (comparing with control group) P＜0.01 table 11 multifunctional nutrient liquid is grown newborn mouse
Annotate: data statistics adopting factor variance analysis method (comparing) P＜0.05 P＜0.01 with control group
| Group | Control group | Low dosage | Middle dosage | High dose |
| 56 days (g) food utilizations (g/100g) of 21 days (g) body weight of body weight | ????12.5±1.6 ????41.8±5.0 ????12.8±3.1 | ???14.8±2.0 ???39.8±0.7 ???12.1±2.4 | ???12.9±0.7 ???40.2±1.6 ???15.3±2.1 | ??12.4±1.9 ??39.9±1.7 ??13.8±2.6 |
| Group | The newborn mouse number of elements | The auricle disengaging time (my god) | The front tooth eruption time (on) (my god) | Front tooth eruption time (descending) (my god) |
| Dosage high dose in the control group low dosage | ????49 ????38 ????44 ????47 | ?????5.1±0.4 ?????5.1±0.1 ?????5.2±0.5 ?????5.1±0.2 | ?????15.4±0.9 ?????12.4±0.5 ?????12.0±0.8 ?????14.4±2.1 | ??????8.5±0.7 ??????7.8±0.7 ??????6.0±0.1 ??????7.6±0.5 |
Result of the test shows:
1, the young situation of tire is not had tangible influence, see Table 9;
2, wean back body weight and food utilization there are raising slightly, see Table 10;
3, the front tooth eruption time is obviously shifted to an earlier date, other do not have obviously difference, see Table 11;
Conclusion: multifunctional nutrient liquid of the present invention has the effect that promotion is grown.
(3) animal experiment of immunologic function regulating action:
Select for use kunming mice to be divided into four groups, high, medium and low dosage group and control group, irritate stomach dosage 30,10,5 (ml/kg) and distilled water respectively every day, continue to kill after 15 days, measure organ coefficient, tardy parasexuality reaction (cellular immune function), antibody forming cell's (humoral immune function), macrophage function, natural killer cell activity.
Result of the test sees Table 12, table 13, table 14, table 15, table 16.
Table 12 multifunctional nutrient liquid is to the influence of mouse thymus weight and coefficient thereof
Table 13 multifunctional nutrient liquid is to the influence (cellular immune function) of the tardy parasexuality reaction of mouse
Table 14 multifunctional nutrient liquid is to mouse spleen antibody forming cell's influence (humoral immune function)
Table 15 multifunctional nutrient liquid is engulfed the influence (macrophage function) of chicken red blood cell ability to Turnover of Mouse Peritoneal Macrophages
Table 16 multifunctional nutrient liquid is to the influence (natural killer cell activity) of mouse spleen NK activity
| Group | Dosage (ml/kg) | Mouse number (only) | Body weight (g) | Thymus gland heavy (mg) | The thymus gland coefficient | The P value |
| Dosage group high dose group in the control group low dose group | ?????0 ?????5 ????10 ????30 | ????10 ????10 ????10 ????10 | ??30.1±3.4 ??31.0±4.2 ??28.9±3.2 ??28.7±4.1 | ??80.1±25.2 ??92.3±23.2 ?111.8±27.2 ??98.1±49.3 | ??0.27±0.09 ??0.31±0.09 ??0.39±0.10 ??0.35±0.19 | ?＞0.05 ?＜0.05 ?＞0.05 |
| Group | Dosage (ml/kg) | Mouse number (only) | ??????DTH(mm) | The P value |
| Dosage group high dose group in the control group low dose group | ?????0 ?????5 ????10 ????30 | ????10 ????10 ????10 ????10 | ????0.44±0.09 ????0.60±0.12 ????0.58±0.18 ????0.52±0.14 | ＞0.05 ＜0.05 ＞0.05 |
| Group | Dosage (ml/kg) | Mouse number (only) | ??IgMPFC/10 6 | The P value |
| Dosage group high dose group in the control group low dose group | ?????0 ?????5 ????10 ????30 | ????10 ????10 ????10 ????10 | ????1062±181 ????1796±707 ????01688±650 ????1230±406 | ＞0.01 ＜0.01 ＞0.05 |
| Group | Dosage (ml/kg) | Mouse number (only) | Phagocytic rate (%) | The P value |
| Dosage group high dose group in the control group low dose group | ?????0 ?????5 ????10 ????30 | ????10 ????10 ????10 ????10 | ????17.5±5.6 ????26.6±8.1 ????29.2±2.8 ????27.1±4.8 | ?＞0.01 ?＜0.01 ?＞0.01 |
| Group | Dosage (ml/kg) | Mouse number (only) | NK activity (%) | The P value |
| Dosage group high dose group in the control group low dose group | ?????0 ?????5 ????10 ????30 | ????10 ????10 ????10 ????10 | ????11.1±5.1 ????17.4±2.2 ????20.7±3.0 ????16.8±3.4 | ?＞0.01 ?＜0.01 ?＞0.01 |
Result of the test shows:
1, multifunctional nutrient liquid of the present invention can obviously increase the heavy and thymus index of mouse thymus, significance statistically, and P＜0.05 sees Table 12.
2, multifunctional nutrient liquid of the present invention can obviously increase delayed hypersensitivity reaction (cellular immune function), and P＜0.05 sees Table 13.
3, multifunctional nutrient liquid of the present invention can obviously increase antibody forming cell's (humoral immune function) of mouse spleen, significance statistically, and P＜0.05 sees Table 14.
4, multifunctional nutrient liquid of the present invention can improve Turnover of Mouse Peritoneal Macrophages and engulfs the red ability (macrophage function) of carefully getting rid of of chicken, and P＜0.05 sees Table 15.
5, multifunctional nutrient liquid of the present invention can improve the natural killer cell activity of mouse spleen, and P＜0.05 sees Table 16.
In sum, multifunctional nutrient liquid of the present invention has immunoregulation effect.
Claims (6)
1, a kind of multifunctional nutrient liquid, it is characterized in that this multifunctional nutrient liquid comprises the aqueous extract 5-15 of (parts by weight) Chinese medicine composition, the pancreatin enzymolysis liquid 13-23 of animal tissue, the albumen conversion fluid 50-80 of soybean germ and the mixed liquor after the bacterium fermentation by fermentation thereof, also contain trace compound and flavor enhancement, this Chinese medicine composition comprises: Radix Codonopsis 40-60, matrimony vine 5-15, mulberry fruit 5-15, date 10-30, this animal tissue comprises: beef 20-30, animal spleen 35-45, animal brain 8-18, this zymophyte comprises: Bifidobacterium 0.1-5, Lactobacillus delbrueckii 0.1-5, saccharomycete 0.01-0.5, soybean germ is 200-400, pancreatin 0.1-10, Na
2CO
30.03-1, minor metallic element compound 0.001-0.1, flavor enhancement 4-50.
2, multifunctional nutrient liquid according to claim 1 is characterized in that: this minor metallic element compound comprises zinc sulfate.
3, multifunctional nutrient liquid according to claim 1 is characterized in that: this flavor enhancement comprises sugar, honey.
4, multifunctional nutrient liquid according to claim 1, it is characterized in that: this selects for use the Chinese medicine composition in the raw material to comprise Radix Codonopsis 50 grams, matrimony vine 10 grams, mulberry fruit 10 grams, date 20 grams, animal tissue comprises: beef 26 grams, pig spleen 39 grams, pig brain 13 grams, zymophyte comprise: Bifidobacterium 5 grams, Lactobacillus delbrueckii 5 grams, saccharomycete 0.2 gram, soybean germ 300 grams, Na
2CO
30.8 gram, pancreatin 5 grams, zinc sulfate 0.04 gram, glucose 15 grams, sucrose 30 grams.
5, multifunctional nutrient liquid according to claim 1 is characterized in that: the material proportion of this nutrient solution is aqueous extract 10 grams, pancreatin enzymolysis liquid 18 grams of animal tissue, albumen conversion fluid 67.2 grams of soybean germ, Bifidobacterium 0.5 gram, Lactobacillus delbrueckii 0.5 gram, Yeast diffusion juice 0.3 gram, zinc sulfate 0.00367 gram, glucose 1.5 grams, sucrose 3 grams of Chinese medicine composition.
6, the manufacture method of multifunctional nutrient liquid according to claim 1 is characterized in that: this manufacture method comprises the steps:
(1) select materials: the raw material of selecting to make multifunctional nutrient liquid comprises (parts by weight): Chinese medicine composition: Radix Codonopsis 40-60, matrimony vine 5-15, mulberry fruit 5-15, date 10-30, the beef 20-30 of animal tissue, animal spleen 35-45, animal brain 8-18, zymophyte: Bifidobacterium 0.1-5, Lactobacillus delbrueckii 0.1-5, saccharomycete 0.01-0.5, soybean germ 200-400, pancreatin 0.1-10, Na
2CO
30.03-1, minor metallic element compound 0.001-0.1, flavor enhancement 4-50;
(2) aqueous extract of preparation Chinese medicine composition:
Above-mentioned Chinese medicine composition was steeped 30-60 minute with the water logging of 4-10 times of weight, the temperature fire was fried in shallow oil 60-90 minute, filtered, and collected aqueous extract for the first time, the water that adds 4-10 times of weight in the medicine quarrel, the temperature fire was fried in shallow oil 60-90 minute, filtered, and collected aqueous extract for the second time, merge aqueous extract twice, be concentrated into the weight of Chinese medicine mixture, make the Chinese medicine aqueous extract, standby;
(3) the pancreatin enzymolysis liquid of preparation animal tissue:
Above-mentioned animal tissue is rubbed with meat grinder, in the 1-4 of animal tissue's weight water doubly, add Na
2CO
3, make its dissolving, add the animal tissue of rubbing, be heated to 90-100 ℃, kept 45-60 minute, be cooled to 40 ℃ subsequently, add pancreatin, enzymolysis 8-10 hour, filter, make the pancreatin enzymolysis liquid of animal tissue, standby;
(4) the albumen conversion fluid of preparation soybean germ:
Get the soybean germ of the long 2-4cm of bud, add water 2-4 doubly, under 100-110 ℃ of condition boiling 30-45 minute, filter, make the albumen conversion fluid of soybean germ, standby;
(5) make multifunctional nutrient liquid:
The albumen conversion fluid of the pancreatin enzymolysis liquid of the aqueous extract of the Chinese medicine composition of above-mentioned (two), (three), (four) preparation, animal tissue and soybean germ merged mix, sterilization treatment under 121 ℃ of conditions is cooled to 40 ℃, adds zymophyte: Bifidobacterium, Lactobacillus delbrueckii and saccharomycete, keep 39 ± 1 ℃, fermented in 15-20 hour, and be warming up to 60 ℃ subsequently, stablized 3 hours, stop fermentation, add trace compound and flavor enhancement, product is made in bottling routinely.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB981008763A CN1135933C (en) | 1998-03-02 | 1998-03-02 | Multifunctional nutritive liquid and its producing method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB981008763A CN1135933C (en) | 1998-03-02 | 1998-03-02 | Multifunctional nutritive liquid and its producing method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1227727A true CN1227727A (en) | 1999-09-08 |
| CN1135933C CN1135933C (en) | 2004-01-28 |
Family
ID=5216310
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB981008763A Expired - Lifetime CN1135933C (en) | 1998-03-02 | 1998-03-02 | Multifunctional nutritive liquid and its producing method |
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| Country | Link |
|---|---|
| CN (1) | CN1135933C (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2930155A1 (en) * | 2008-04-21 | 2009-10-23 | Oreal | USE OF A BAY EXTRACT, AND MORE PARTICULARLY WOLFBERRY, AS ANTI-POLLUTION AGENT |
| US20110162981A1 (en) * | 2008-07-30 | 2011-07-07 | Yuanlong Pan | Methods and compositions for preventing, reducting or treating damage caused by ischemia and schemia-like conditions |
| CN104522815A (en) * | 2015-01-14 | 2015-04-22 | 三株福尔制药有限公司 | Probiotics fermentation maca composition and preparing method and application thereof |
-
1998
- 1998-03-02 CN CNB981008763A patent/CN1135933C/en not_active Expired - Lifetime
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2930155A1 (en) * | 2008-04-21 | 2009-10-23 | Oreal | USE OF A BAY EXTRACT, AND MORE PARTICULARLY WOLFBERRY, AS ANTI-POLLUTION AGENT |
| WO2009141543A1 (en) * | 2008-04-21 | 2009-11-26 | L'oreal | Use of a berry extract, especially a wolfberry extract, as an anti-pollutant |
| US20110162981A1 (en) * | 2008-07-30 | 2011-07-07 | Yuanlong Pan | Methods and compositions for preventing, reducting or treating damage caused by ischemia and schemia-like conditions |
| CN104522815A (en) * | 2015-01-14 | 2015-04-22 | 三株福尔制药有限公司 | Probiotics fermentation maca composition and preparing method and application thereof |
| CN104522815B (en) * | 2015-01-14 | 2016-07-06 | 三株福尔制药有限公司 | A kind of probiotics fermention maca composition and its preparation method and application |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1135933C (en) | 2004-01-28 |
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Effective date of registration: 20160811 Address after: Jiyang County, Ji'nan City, Shandong province 251402 Jibei Development Zone Industrial Park Sun Geng prosperous Street No. 2 Patentee after: SANZHU FUER PHARMACEUTICAL CO., LTD. Address before: 250100 No. 77 North Grand Road, Shandong, Ji'nan Patentee before: Sanzhu Pharmaceutical Industry Co., ltd., Jinan City |
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