CN1227262C - 一种与植物细胞周期相关的蛋白质和其编码基因 - Google Patents
一种与植物细胞周期相关的蛋白质和其编码基因 Download PDFInfo
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Abstract
本发明公开了一种存在于植物中的与细胞周期相关的基因CCR及所编码的蛋白质。它参予细胞周期中的G1期至S期。转基因实验证明该基因的超量表达促使植物的生长和发育，转基因植株的长势明显好于对照且比对照提前开花和结实。转反义基因的植株则矮小且发育缓慢。与对照相比，超量表达该基因的转基因植株还有持久的耐盐性。本发明还提供了一种利用CCR基因进行植物品种改良的方法。
Description
技术领域
本发明属于基因工程领域，具体地讲涉及植物中的一类新的细胞周期相关基因及其所编码的蛋白质。
技术背景
细胞分裂是生命的最重要且普遍存在的特征。细胞周期过程是一个基础而复杂的过程。在真核和原核生物中存在着一些相当保守的元件控制着细胞的周期。在酵母和人类中，细胞周期(S，G2，M和G1)的完成与一系列丝/苏氨酸激酶相关。这些激酶包括催化亚基，称为依赖细胞周期的激酶，cyclin-dependent kinase(CDK)和一个活化亚基，cyclin。七十年代，首次运用酵母突变体研究细胞周期调控的分子机制，鉴定了调控复合物的主要分子细胞周期蛋白CDC2和CDC28，与此同时，在海胆中也鉴定了另一个重要分子依赖于细胞周期蛋白的激酶。在过去的30年中，酵母和哺乳类的细胞周期调控研究得到了长足的进展。1989年，John等人用免疫学方法在植物中发现了类似CDK的存在。之后，1990年，从豌豆中克隆了类CDK蛋白的基因。随之，又有一些CDK和cyclin被相继发现。十年来已从各种植物中克隆了cyclin，CDK，CDK-活化激酶CAK和CDK抑制物CKI基因，并且勾画了简单的植物细胞周期调控的遗传机制。
在细胞周期调控中，G1到S相是最重要的。在G1相至细胞分裂中已发现了许多基因涉及调控作用。在酵母和动物中，这些基因的突变引起表型变化甚至死亡。拟南芥中，cyclinD在G1到S相时表达并起作用。
发明内容
本发明人从盐生植物山菠菜Atriplex hortensis中克隆了一个cDNA，与上述cyclinD基因不同源。经在数据库中比较它的编码蛋白与人类中编码G1至S的细胞周期蛋白p38-2G4(AAB91536)有50％相似性，因此命名为AhCCR(Cell Cycle Related)基因。AhCCR cDNA全长1460bp，含有1194bp的ORF，编码含397个氨基酸的蛋白质，5’-UTR为82bp，3’-UTR为184bp。
以AhCCR基因序列从拟南芥基因组序列中查出其同源基因序列，据此设计引物，从拟南芥cDNA中克隆了AhCCR的同源基因AtCCR基因。AtCCR cDNA全长1472bp，含有1179bp的ORF，编码392个氨基酸，5’-UTR为96bp，3’-UTR为197bp。
转基因研究表明，无论将AhCCR基因转入烟草还是将AtCCR基因转入拟南芥，转基因植株在正常条件下的长势明显优于对照且提前开花结实。而以反义AtCCR转化的拟南芥生长缓慢。AtCCR基因过量表达的转基因植株比对照有明显高的耐逆性。该基因可作为目的基因导入植物，提高植物的产量及耐逆性，以进行植物品种改良。
综上所述，本发明人提供的CCR基因是植物中的一类新基因，其参予植物细胞周期的调控。
因而，本发明的一个目的是提供一种新的参予植物细胞周期调控的基因的核苷酸序列，此类基因中的AhCCR、AtCCR分别如序列表1，2所示。
本发明所述的一种新的参予植物细胞周期调控的蛋白质(基因)可以是来自所有植物包括山菠菜、烟草、拟南芥、草坪草、杨树、大豆、小麦、水稻、棉花或蕃茄等。这种蛋白质的氨基酸序列中AhCCR、AtCCR分别如序列表1，2所示。
本发明的再一个目的是提供一种培育耐逆植物品种的方法，包括将本发明所述的植物细胞周期相关基因导入植物细胞并使其表达的步骤。
在本发明的方法中，可利用CCR基因作为目的基因构建植物表达载体，其中可用任何一种启动子例如花椰菜花叶病毒(CAMV)35S启动子、Ubiquitin启动子或其它启动子，该表达载体中必要时可包括增强子，不论是转录增强子或翻译增强子。为了简化转化细胞的鉴定可使用选择性标记包括对抗生素抗性的酶，也可利用颜色变化(例如B-葡糖醛酸糖苷酶GUS)或发光(例如荧光酶)来识别的化合物的酶类，也可用无标记选择。所用的表达载体可使用Ti质粒，Ri质粒，植物病毒载体等。转化方法可用经农杆菌介导法、基因枪法、花粉管通道法或其它方法转化植物。
本发明的CCR基因的受体植物包括单子叶和双子叶植物， 例如水稻、小麦、玉米、大豆、棉花、蔬菜、树木和草坪草等。
附图说明
图1.AhCCR基因与人类盘p38-2G4基因的相似性比较。
图2.AhCCR基因的Southem分析。
图3.CCR基因的植物表达载体构建。
图4.转AhCCR基因烟草在无胁迫下与对照的比较。
图5.转正义、反义AtCCR基因的拟南芥植株在无胁迫下与对照的比较。
图6.转AtCCR基因植株在盐胁迫下与对照的比较。
具体实施方式
实施例1
山菠菜种于温室中(22℃)，6星期后以400mM NaCl浇灌，4天后取叶子，分离mRNA，按常规方法构建cDNA文库。以AhDREB(已申请发明专利，申请号：01123827.5)的240bp片段为探针从1.5×106噬斑中以低严谨条件筛选到46个噬斑。经过3轮筛选后取插入片段大于1.0Kb的进行序列分析。其中一个片段约1.5Kb，包括1194bp ORF，82bp 5’-UTR及184bp 3’-UTR(序列表1)。GeneBack blast比较发现该片段与人类中G1至S细胞周期蛋白p38-2G4基因相似性为51％。(图1)。
实施例2
根据AhCCR基因序列，在GeneBack数据库中从拟南芥基因组全序列中检索到相应的基因AtCCR的序列，据此，用PCR方法从拟南芥cDNA中扩增并克隆了AtCCR基因。其氨基酸和核苷酸序列列于序列表2。根据序列检索表明AtCCR基因在拟南芥基因组中也仅有一个拷贝。
实施例3
用实例1中的山菠菜材料提取的总DNA进行Southern分析表明在山菠菜基因组中仅有一个拷贝AhCCR基因(图2)。以AhCCR基因序列检索GeneBack表明在拟南芥基因组中也仅有一个拷贝AtCCR基因。
实施例4
用实验例1中所得到的AhCCR基因构建植物表达载体(图3)，以农杆菌介导法转化烟草。转基因烟草的生长明显优于对照，发育(开花)也比对照提前(图4)。
实施例5
以实验例2中得到的AtCCR基因，按实验例4的方法构建AtCCR基因正义和反义的植物表达载体并分别转化拟南芥，转正义基因也即AtCCR基因超表达的转基因植株生长和发育均优于对照，而转反义基因的植株也即AtCCR基因低表达的植株生长与发育均明显劣于对照(图5)。
上述实施例表明本发明中克隆的这一类新的植物细胞基因CCR具有与细胞周期相关的特征，与人类中的同类蛋白p38-2G4的相似性为51％，此类基因在植物中的功能迄今未经描述，为一类新基因。实验例4、5表明该基因与植物的生长、发育以及对逆境的耐性相关。此类基因可作为目的基因用以改良植物的品种。
序列表1
-81 TGCACCGTTCACCTCTATTTTCCATTTTTCTGCTATTTACAAACGAAAGCGATAAACATTTTGATTACTTTG
-9 TTGAAGAATAATGTCGGACGATGAGAGAGAGGAGAAGGAGTTGGATCTTAGCTATCCTGAAGTTGTTACCAA
M S D D E R E E K E L D L S Y P E V V T K
64 ATACAAGAGCGCTGCTGACATTGTCAACAGAGCTTTGCAGCTAGTTGTCGGAGAATGCAAGCCCAAAGCTAA
Y K S A A D I V N R A L Q L V V G E C K P K A K
136 AATTGTTGATTTGTGCGAGAAAGGGGATAATTTTATCCGAGAGCAAACTGGTAATATGTACAAGAATGTGAA
I V D L C E K G D N F I R E Q T G N M Y K N V K
208 GAAGAAGATTGAACGAGGTGTTGCTTTTCCAACTTGTTTATCTATCAACAATGTTGTTTGTCATTTCTCACC
K K I E R G V A F P T C L S I N N V V C H F S P
280 ACTTGCCAGTGATGACTCAGTGCTTGAAGAAGGTGATTTAGTGAAAATTGATATGGGTTGTCATATTGATGG
L A S D D S V L E E G D L V K I D M G C H I D G
352 CTTTATTGCTGTTGTTGCACACACTCATGTCATTCAAGAAGGTCCGGTAACCGGTAGAAAAGCTGATGTTCT
F I A V V A H T H V I Q E G P V T G R K A D V L
424 TGCAGCAACCAACACTGCTGCTGAGGTTGCTTTGAGGCTTGTGAGGCCTGGAAAAAACCAATATAGATGTAA
A A T N T A A E V A L R L V R P G K N Q Y R C N
496 CCAGAAGCAATCCCAGAAAGTGCTGCAGCATATGACTGTAAAATTGTTGAAGGTGTCCTTAGTCACCAAATG
Q K Q S Q K X A A A Y D C K I V E G V L S H Q M
568 AAACAGTTCGTGATTGATGGAAACAAGGTGGTGTTGAGTGTATCAAGTCCTGATGTGAGAGTTGATGATGCT
K Q F V I D G N K V V L S V S S P D V R V D D A
640 GAATTTGAGGAGAACGAAGTCTATTCTGTTGATATAGCTGCTAGTACGGGTGAGGGAAAGCCTAGAATGTTG
E F E E N E V Y S V D I A A S T G E G K P R M L
712 GACGAGAAGCAAACAACTATTTACAAAAGAGCTGTGGACAAGAATTATCACTTGAAAATGAAAGCATCAAGA
D E K Q T T I Y K R A V D K N Y H L K M K A S R
784 TTTATTTTCAGTGAAATCAATCAGAAATTCCCTATCATGCCATTTACAGCAAGGGcGTTGGAGGAGAAGAGG
F I F S E I N Q K F P I M P F T A R A L E E K R
856 GCTCGTCTTGGACTTGTAGAATGTGTCAATCACGACCTCTTGCAACCCTATCCTGTTTTGTATGAGAAACCT
A R L G L V E C V N H D L L Q P Y P V L Y E K P
928 GGAGATTATGTTGCTCACATAAAATTCACTGTTCTTCTCATGCCAAATGGATCGGATCGTGTGACATCATAT
G D Y V A H I K F T V L L M P N G S D R V T S Y
1000 CCTGTGCAAGAATTGCAGCCAACTAAATCAATTGACGACCCTGAAATTAAAGCTTGGTTAGCATTGGGAACA
P V Q E L Q P T K S I D D P E I K A W L A L G T
1072 AAGACAAAGAAGAAAGGTGGTGGTAAAAAGAAGAAAGCCAAGAAGGGTGAAAAATCTGGTGCCGAAGAGTCA
K T K K K G G G K K K K A K K G E K S G A E E S
1144 GCTGATGCAGAGCCCATGGATACAAGTGCAAATGGTGCAGCTGAAAATTAAAAAGACAAAAAAGTTTGTTCA
A D A E P M D T S A N G A A E N *
1216 AATTTTGTTACTTGTTGATTCTCTTTTGTATTTGCTTGATAGGCAACTTAAACATGTTTAGATGTTGATCTT
1288 CAATATTTCATGTTGCCTCTCACCTGTGTAAAGTTTCTGGGTTTGAGCCCACAAAATGAATTTTTTCTTGAT
1360 ACAAAAACGACAGCAACGG
序列表2
Nucleotide-Protein
1 CATTTCTCTCCTTCTCCACCAGTTTTCCCCCAATCGTTCCTCCTTAAACCCTAACATTTT
61 TGCTTCCCCTGTTTCGATTCTCGAGAGTTATCAGAGATGAGTTCGGACGATGAGAGAGAC
M S S D D E R D
121 GAGAAGGAGCTGAGTCTTACCTCTCCTGAAGTCGTCACCAAGTACAAGAGCGCCGCTGAG
E K E L S L T S P E V V T K Y K S A A E
181 ATCGTTAACAAGGCGTTACAGGTTGTTTTAGCTGAATGCAAACCAAAAGCTAAGATTGTT
I V N K A L Q V V L A E C K P K A K I V
241 GATATCTGTGAGAAAGGAGACTCTTTTATTAAAGAGCAAACAGCAAGCATGTACAAGAAT
D I C E K G D S F I K E Q T A S M Y K N
301 TCTAAGAAGAAGATTGAGAGAGGTGTTGCGTTCCCTACATGCATTTCTGTGAACAACACT
S K K K I E R G V A F P T C I S V N N T
361 GTTGGTCATTTTTCACCGCTTGCTAGTGATGAGTCTGTGTTGGAAGATGGTGACATGGTT
V G H F S P L A S D E S V L E D G D M V
421 AAAATCGATATGGGATGTCATATTGATGGGTTCATTGCCCTTGTTGGTCACACACATGTT
K I D M G C H I D G F I A L V G H T H V
481 CTTCAAGAAGGGCCTCTTAGTGGGCGTAAGGCTGATGTTATCGCTGCCGCAAATACTGCA
L Q E G P L S G R K A D V I A A A N T A
541 GCTGATGTTGCTCTAAGGCTCGTACGTCCTGGAAAAAAGAACACTGATGTAACTGAAGCT
A D V A L R L V R P G K K N T D V T E A
601 ATTCAGAAGGTAGCTGCAGCTTATGACTGCAAAATTGTTGAAGGTGTTCTTTCCCACCAG
I Q K V A A A Y D C K I V E G V L S H Q
661 CTGAAACAGCATGTGATAGATGGAAACAAGGTTGTGCTTAGTGTATCCAGCCCTGAAACA
L K Q H V I D G N K V V L S V S S P E T
721 ACTGTTGACGAAGTGGAATTTGAAGAGAATGAAGTCTATGCAATAGATATTGTGGCAAGT
T V D E V E F E E N E V Y A I D I V A S
781 ACTGGTGATGGCAAGCCAAAGCTATTAGACGAGAAGCAAACAACTATTTACAAGAAAGAT
T G D G K P K L L D E K Q T T I Y K K D
841 GAAAGTGTTAACTATCAGTTGAAGATGAAGGCCTCCAGATTCATAATCAGCGAAATTAAA
E S V N Y Q L K M K A S R F I I S E I K
901 CAGAACTTCCCCCGTATGCCATTCACTGCAAGGTCACTGGAGGAGAAAAGGGCACGGCTT
Q N F P R M P F T A R S L E E K R A R L
961 GGACTTGTGGAGTGTGTGAACCATGGTCATTTGCAACCATATCCTGTTCTTTACGAGAAG
G L V E C V N H G H L Q P Y P V L Y E K
1021 CCTGGGGATTTTGTTGCTCAGATTAAGTTCACAGTTTTGCTGATGCCAAATGGATCAGAT
P G D F V A Q I K F T V L L M P N G S D
1081 AGGATCACTTCACATACACTTCAGGAACTTCCTAAAAAGACCATCGAAGACCCTGAGATC
R I T S H T L Q E L P K K T I E D P E I
1141 AAACGGTGGTTAGCTTTGGGCATCAAGAAGAAGAAAGGTGGTGGAAAGAAGAAGAAAGCC
K G W L A L G I K K K K G G G K K K K A
1201 CAAAAGGCGGGAGAGAAAGGAGAGGCTTCAACAGAGGCTGAGCCAATGGACGCAAGTAGT
Q K A G E K G E A S T E A E P M D A S S
1261 AATGCTCAAGAATGAGTAGGAAGATTTGTCTTTGTTTCTTTTAAAGAATTTTTGAAACTT
N A Q E
1321 GAGGTACCTCTTTCGTTGTATGTTTGGGTTTCAAGAGAGTCTCTTCTTAAACAAAAAAAA
1381 ACAAACAATAGTTTACCACCTTTGTGCATATTTTTGCACAAAGCACTTTTCATCATCATT
1441 TCTTTGCTTGTCCTAATCTTATCTCATTCTCT
序列表3
Nucleotide-Protein
-90 GCACGAGCGACCTCCCCTTCCTCCGCCGCCGCCGCCACCGCCACCGCCACCGCCACGTCC
-30 AGCCTCCGGTCGCCTGTTCGGATCGAAGCGATGTCGTCGGACGATGAGGTCAGAGAGGAG
M S S D D E V R E E
31 AAGGAGCTCGACCTCTCCTCCTCCGACGTCGTCACCAAGTACAAGGACGCCGCCGATATC
K E L D L S S S D V V T K Y K D A A D I
91 ATCAACAATGCTCTTAAGTTGGTAGTGTCTTTGTGCAAGCCGAAAGCCAAGATTGTTGAC
I N N A L K L V V S L C K P K A K I V D
151 ATCTGTGAGAAGGGCGATTCTTACATAAGAGAGCAAACTGGCAACATCTACAAGAATGTG
I C E K G D S Y I R E Q T G N I Y K N V
211 AAGAGGAAGATTGAAAGAGGTGTTGCTTTTCCAACATGTGTNTCTGTGAACAACACAGTC
K R K I E R G V A F P T C V S V N N T V
271 TGCCATTTCTCTCCGCTAGCCACTGATGAGGCAGTCCTGGAGGAAAATGATATGGTCAAA
C H F S P L A T D E A V L E E N D M V K
331 ATTGATATGGGATGTCATATTGATGGTTTTATTGCCGTGGTGGCTCATACACATGTTATC
I D M G C H I D G F I A V V A H T H V I
391 CATGATGGAGCAGTCACTGGGAAAGCAGCAGATGTTCTTGCTGCTGCCAACACAGCGGCA
H D G A V T G K A A D V L A A A N T A A
451 GAAGTTGCTCTGAGGTTTGTTAGACCTGGGAAGAAGAACAAGGATGTCACCGAAGCGATT
E V A L R F V R P G K K N K D V T E A I
511 CAGAAAGTTGCTGCTGCCTATGACTCCGTATCTGTTGAAGGAGTTCTCAGCCATCAGCTG
Q K V A A A Y D S V S V E G V L S H Q L
571 AAACAGTTTGTCATTGACGGTAACAAAGTTGTACTTAGTGTGTCCAATGCTGATACGAAG
K Q F V I D G N K V V L S V S N A D T K
631 GTGGATGATGCTGAATTTGAGGAAAATGAAGTGTATGCAATTGATATTGTCACCAGCACT
V D D A E F E E N E V Y A I D I V T S T
691 GGAGAAGGGAAGCCCAAGTTATTGGATGAGAAGCAGACCACCATCTACAAGAGAGCTGTA
G E G K P K L L D E K Q T T I Y K R A V
751 GACAAGAATTATCACTTGAAGATGAAAGCATCTAGGTTCATTTTCAGTGAGATAAGCCAG
D K N Y H L K M K A S R F I F S E I S Q
811 AAGTTTCCTATCATGCCCTTCACTGCTAGGGCATTGGAAGAGAAGCGTGCACGCTTAGGT
K F P I M P F T A R A L E E K R A R L G
871 TTGGTGGAATGCATGAATCATGAATTATTGCAGCCGTACCCTGTCCTTCACGAGAAGCCA
L V E C M N H E L L Q P Y P V L H E K P
931 GGCGACCTTGTTGCACACATCAAGTTTACTGTGCTTCTGCTGCCATCTGGTTCACAGAGG
G D L V A H I K F T V L L L P S G S Q R
991 GTAACATCGCATTCACTTCAGGTGTTGCAGCCCACTAAATCCATTGAAGACCACGCTGAG
V T S H S L Q V L Q P T K S I E D H A E
1051 ATCAAGGCATGGCTTGCTTTGGGGACCAAAACAAAGAAGAAAAGTGGTGCCCAAAAGAAG
I K A W L A L G T K T K K K S G A Q K K
1111 AAAGGTAAGAAAGGAGATGCAGCAGAAGCAGTGCCTATGGAGGAGGGTTCAAATGATGCA
K G K K G D A A E A V P M E E G S N D A
1171 AACAAAGAATAACTGATTGTTCTTTTGAATTGCTTCCGTTAGTTTTGTACTTGCTCCACT
N K E
1231 GCATCTGATGCTATTATATCACATTCAGTTGTGGAAAATCGAGTACTTTTTCAAACTTCC
1291 TGGGAAATGATCCGTCCTGCTGCACACTCATTGTCCAAAAAA
CCR专利
SEQUENCE LISTING
<110>中科院遗传发育所
<120>一种与细胞周期相关的蛋白质和其编码基因
<130>一种与细胞周期相关的蛋白质和其编码基因
<140>02108797.0
<141>2002-07-01
<160>6
<170>PatentIn version 3.1
<210>1
<211>1194
<212>DNA
<213>Atriplex hortensis
<220>
<221>CDS
<222>(1)..(1191)
<223>
<400>1
atg tcg gac gat gag aga gag gag aag gag ttg gat ctt agc tat cct 48
Met Ser Asp Asp Glu Arg Glu Glu Lys Glu Leu Asp Leu Ser Tyr Pro
1 5 10 15
gaa gtt gtt acc aaa tac aag agc gct gct gac att aac aac aga gct 96
Glu Val Val Thr Lys Tyr Lys Ser Ala Ala Asp Ile Val Asn Arg Ala
20 25 30
ttg cag cta gtt gtc gga gaa tgc aag ccc aaa gct aaa att gtt gat 144
Leu Gln Leu Val Val Gly Glu Cys Lys Pro Lys Ala Lys Ile Val Asp
35 40 45
ttg tgc gag aaa ggg gat aat ttt atc cga gag caa act ggt aat atg 192
Leu Cys Glu Lys Gly Asp Asn Phe Ile Arg Glu Gln Thr Gly Asn Met
50 55 60
tac aag aat gtg aag aag aag att gaa cga ggt gtt gct ttt cca act 240
Tyr Lys Asn Val Lys Lys Lys Ile Glu Arg Gly Val Ala Phe Pro Thr
65 70 75 80
tgt tta tct atc aac aat gtt ggt tgt cat ttc tca cca ctt gcc agt 288
Cys Leu Ser Ile Asn Asn Val Val Cys His Phe Ser Pro Leu Ala Ser
85 90 95
gat gac tca gtg ctt gaa gaa ggt gat tta gtg aaa att gat atg ggt 336
Asp Asp Ser Val Leu Glu Glu Gly Asp Leu Val Lys Ile Asp Met Gly
100 105 110
tgt cat att gat ggc ttt att gct gtt gtt gca cac act cat gtc att 384
Cys His Ile Asp Gly Phe Ile Ala Val Val Ala His Thr His Val Ile
115 120 125
caa gaa ggt ccg gta acc ggt aga aaa gct gat gtt ctt gca gca acc 432
Gln Glu Gly Pro Val Thr Gly Arg Lys Ala Asp Val Leu Ala Ala Thr
130 135 140
aac act gct gct gag gtt gct ttg agg ctt gtg agg cct gga aaa aac 480
Asn Thr Ala Ala Glu Val Ala Leu Arg Leu Val Arg Pro Gly Lys Asn
145 150 155 160
caa tat aga tgt aac cag aag caa tcc cag aaa gkt gct gca gca tat 528
Gln Tyr Arg Cys Asn Gln Lys Gln Ser Gln Lys Xaa Ala Ala Ala Tyr
165 170 175
gac tgt aaa att gtt gaa ggt gtc ctt agt cac caa atg aaa cag ttc 576
Asp Cys Lys Ile Val Glu Gly Val Leu Ser His Gln Met Lys Gln Phe
180 185 190
gtg att gat gga aac aag gtg gtg ttg agt gta tca agt cct gat gtg 624
CCR专利
Val Ile Asp Gly Asn Lys Val Val Leu Ser Val Ser Ser Pro Asp Val
195 200 205
aga gtt gat gat gct gaa ttt gag gag aac gaa gtc tat tct gtt gat 672
Arg Val Asp Asp Ala Glu Phe Glu Glu Asn Glu Val Tyr Ser Val Asp
210 215 220
ata gct gct agt acg ggt gag gga aag cct aga atg ttg gac gag aag 720
Ile Ala Ala Ser Thr Gly Glu Gly Lys Pro Arg Met Leu Asp Glu Lys
225 230 235 240
caa aca act att tac aaa aga gct gtg gac aag aat tat cac ttg aaa 768
Gln Thr Thr Ile Tyr Lys Arg Ala Val Asp Lys Asn Tyr His Leu Lys
245 250 255
atg aaa gca tca aga ttt att ttc agt gaa atc aat cag aaa ttc cct 816
Met Lys Ala Ser Arg Phe Ile Phe Ser Glu Ile Asn Gln Lys Phe Pro
260 265 270
atc atg cca ttt aca gca agg gcg ttg gag gag aag agg gct cgt ctt 864
Ile Met Pro Phe Thr Ala Arg Ala Leu Glu Glu Lys Arg Ala Arg Leu
275 280 285
gga ctt gta gaa tgt gtc aat cac gac ctc ttg caa ccc tat cct gtt 912
Gly Leu Val Glu Cys Val Asn His Asp Leu Leu Gln Pro Tyr Pro Val
290 295 300
ttg tat gag aaa cct gga gat tat gtt gct cac ata aaa ttc act gtt 960
Leu Tyr Glu Lys Pro Gly Asp Tyr Val Ala His Ile Lys Phe Thr Val
305 310 315 320
ctt ctc atg cca aat gga tcg gat cgt gtg aca tca tat cct gtg caa 1008
Leu Leu Met Pro Asn Gly Ser Asp Arg Val Thr Ser Tyr Pro Val Gln
325 330 335
gaa ttg cag cca act aaa tca att gac gac cct gaa att aaa gct tgg 1056
Glu Leu Gln Pro Thr Lys Ser Ile Asp Asp Pro Glu Ile Lys Ala Trp
340 345 350
tta gca ttg gga aca aag aca aag aag aaa ggt ggt ggt aaa aag aag 1104
Leu Ala Leu Gly Thr Lys Thr Lys Lys Lys Gly Gly Gly Lys Lys Lys
355 360 365
aaa gcc aag aag ggt gaa aaa tct ggt gcc gaa gag rca gct gat gca 1152
Lys Ala Lys Lys Gly Glu Lys Ser Gly Ala Glu Glu Ser Ala Asp Ala
370 375 380
gag ccc atg gat aca agt gca aat ggt gca gct gaa aat taa 1194
Glu Pro Met Asp Thr Ser Ala Asn Gly Ala Ala Glu Asn
385 390 395
<210>2
<211>397
<212>PRT
<213>Atriplex hortensis
<220>
<221>misc feature
<222>(172)..(172)
<223>The’Xaa’at location 172 stands for Gly，or Val.
<400>2
Met Ser Asp Asp Glu Arg Glu Glu Lys Glu Leu Asp Leu Ser Tyr Pro
1 5 10 15
Glu Val Val Thr Lys Tyr Lys Ser Ala Ala Asp Ile Val Asn Arg Ala
20 25 30
Leu Gln Leu Val Val Gly Glu Cys Lys Pro Lys Ala Lys Ile Val Asp
35 40 45
CCR专利
Leu Cys Glu Lys Gly Asp Asn Phe Ile Arg Glu Gln Thr Gly Asn Met
50 55 60
Tyr Lys Asn Val Lys Lys Lys Ile Glu Arg Gly Val Ala Phe Pro Thr
65 70 75 80
Cys Leu Ser Ile Asn Asn Val Val Cys His Phe Ser Pro Leu Ala Ser
85 90 95
Asp Asp Ser Val Leu Glu Glu Gly Asp Leu Val Lys Ile Asp Met Gly
100 105 110
Cys His Ile Asp Gly Phe Ile Ala Val Val Ala His Thr His Val Ile
115 120 125
Gln Glu Gly Pro Val Thr Gly Arg Lys Ala Asp Val Leu Ala Ala Thr
130 135 140
Asn Thr Ala Ala Glu Val Ala Leu Arg Leu Val Arg Pro Gly Lys Asn
145 150 155 160
Gln Tyr Arg Cys Asn Gln Lys Gln Ser Gln Lys Xaa Ala Ala Ala Tyr
165 170 175
Asp Cys Lys Ile Val Glu Gly Val Leu Ser His Gln Met Lys Gln Phe
180 185 190
Val Ile Asp Gly Asn Lys Val Val Leu Ser Val Ser Ser Pro Asp Val
195 200 205
Arg Val Asp Asp Ala Glu Phe Glu Glu Asn Glu Val Tyr Ser Val Asp
210 215 220
Ile Ala Ala Ser Thr Gly Glu Gly Lys Pro Arg Met Leu Asp Glu Lys
225 230 235 240
Gln Thr Thr Ile Tyr Lys Arg Ala Val Asp Lys Asn Tyr His Leu Lys
245 250 255
Met Lys Ala Ser Arg Phe Ile Phe Ser Glu Ile Asn Gln Lys Phe Pro
260 265 270
Ile Met Pro Phe Thr Ala Arg Ala Leu Glu Glu Lys Arg Ala Arg Leu
275 280 285
Gly Leu Val Glu Cys Val Asn His Asp Leu Leu Gln Pro Tyr Pro Val
290 295 300
Leu Tyr Glu Lys Pro Gly Asp Tyr Val Ala His Ile Lys Phe Thr Val
305 310 315 320
Leu Leu Met Pro Asn Gly Ser Asp Arg Val Thr Ser Tyr Pro Val Gln
325 330 335
Glu Leu Gln Pro Thr Lys Ser Ile Asp Asp Pro Glu Ile Lys Ala Trp
340 345 350
CCR专利
Leu Ala Leu Gly Thr Lys Thr Lys Lys Lys Gly Gly Gly Lys Lys Lys
355 360 365
Lys Ala Lys Lys Gly Glu Lys Ser Gly Ala Glu Glu Ser Ala Asp Ala
370 375 380
Glu Pro Met Asp Thr Ser Ala Asn Gly Ala Ala Glu Asn
385 390 395
<210>3
<211>1179
<212>DNA
<213>Arabidopsis thaliana
<220>
<221>CDS
<222>(1)..(1176)
<223>
<400>3
atg agt tcg gac gat gag aga gac gag aag gag ctg agt ctt acc tct 48
Met Ser Ser Asp Asp Glu Arg Asp Glu Lys Glu Leu Ser Leu Thr Ser
1 5 10 15
cct gaa gtc acc acc aag tac aag agc gcc gct gag atc gtt aac aag 96
Pro Glu Val Val Thr Lys Tyr Lys Ser Ala Ala Glu Ile Val Asn Lys
20 25 30
gcg tta cag gtt gtt tta gct gaa tgc aaa cca aaa gct aag att gtt 144
Ala Leu Gln Val Val Leu Ala Glu Cys Lys Pro Lys Ala Lys Ile Val
35 40 45
gat atc tgt gag aaa gga gac tct ttt att aaa gag caa aca gca agc 192
Asp Ile Cys Glu Lys Gly Asp Ser Phe Ile Lys Glu Gln Thr Ala Ser
50 55 60
atg tac aag aat tct aag aag aag att gag aga ggt gtt gcg ttc cct 240
Met Tyr Lys Asn Ser Lys Lys Lys Ile Glu Arg GlV Val Ala Phe Pro
65 70 75 80
aca tgc att tct gtg aac aac act gtt ggt cat ttt rca ccg ctt gct 288
Thr Cys Ile Ser Val Asn Asn Thr Val Gly His Phe Ser Pro Leu Ala
85 90 95
agt gat gag tct gtg ttg gaa gat ggt gac atg gtt aaa atc gat atg 336
Ser Asp Glu Ser Val Leu Glu Asp Gly Asp Met Val Lys Ile Asp Met
100 105 110
gga tgt cat att gat ggg ttc att gcc ctt gtt ggt cac aca cat gtt 384
Gly Cys His Ile Asp Gly Phe Ile Ala Leu Val Gly His Thr His Val
115 120 125
ctt caa gaa ggg cct ctt agt ggg cgt aag gct gat gtt atc gct gcc 432
Leu Gln Glu Gly Pro Leu Ser Gly Arg Lys Ala Asp Val Ile Ala Ala
130 135 140
gca aat act gca gct gat gtt gct cta agg ctc gta cct cct gga aaa 480
Ala Asn Thr Ala Ala Asp Val Ala Leu Arg Leu Va1 Arg Pro Gly Lys
145 150 155 160
aag aac act gat gta act gaa gct att cag aag gta gct gca gct tat 528
Lys Asn Thr Asp Val Thr Glu Ala Ile Gln Lys Val Ala Ala Ala Tyr
165 170 175
gac tgc aaa att gtt gaa ggt gtt ctt tcc cac cag ctg aaa cag cat 576
Asp Cys Lys Ile Val Glu Gly Val Leu Ser His Gln Leu Lys Gln His
180 185 190
gtg ata gat gga aac aag gtt gtg ctt agt gta tcc agc cct gaa aca 624
Val Ile Asp Gly Asn Lys Val Val Leu Ser Val Ser Ser Pro Glu Thr
CCR专利
195 200 205
act gtt gac gaa gtg gaa ttt gaa gag aat gaa gta tat gca ata gat 672
Thr Val Asp Glu Val Glu Phe Glu Glu Asn Glu Val Tyr Ala Ile Asp
210 215 220
att gtg gca agt act ggt gat ggc aag cca aag cta tta gac gag aag 720
Ile Val Ala Ser Thr Gly Asp Gly Lys Pro Lys Leu Leu Asp Glu Lys
225 230 235 240
caa aca act att tac aag aaa gat gaa agt gtt aac tat cag ttg aag 768
Gln Thr Thr Ile Tyr Lys Lys Asp Glu Ser Val Asn Tyr Gln Leu Lys
245 250 255
atg aag gcc tcc aga ttc ata atc agc gaa att aaa cag aac ttc ccc 816
Met Lys Ala Ser Arg Phe Ile Ile Ser Glu Ile Lys Gln Asn Phe Pro
260 265 270
cgt atg cca ttc act gca agg tca ctg gag gag aaa agg gca cgg ctt 864
Arg Met Pro Phe Thr Ala Arg Ser Leu Glu Glu Lys Arg Ala Arg Leu
275 280 285
gga ctt gtg gag tgt gtg aac cat ggt cat ttg caa cca tat cct gtt 912
Gly Leu Val Glu Cys Val Asn His Gly His Leu Gln Pro Tyr Pro Val
290 295 300
ctt tac gag aag cct ggg gat ttt gtt gct cag att aag ttc aca gtt 960
Leu Tyr Glu Lys Pro Gly Asp Phe Val Ala Gln Ile Lys Phe Thr Val
305 310 315 320
ttg ctg atg cca aat gga tca gat agg atc act tca cat aca ctt cag 1008
Leu Leu Met Pro Asn Gly Ser Asp Arg Ile Thr Ser His Thr Leu Gln
325 330 335
gaa ctt cct aaa aag acc atc gaa gac cct gag atc aaa ggg tgg tta 1056
Glu Leu Pro Lys Lys Thr Ile Glu Asp Pro Glu Ile Lys Gly Trp Leu
340 345 350
gct ttg ggc atc aag aag aag aaa ggt ggt gga aag aag aag aaa gcc 1104
Ala Leu Gly Ile Lys Lys Lys Lys Gly Gly Gly Lys Lys Lys Lys Ala
355 360 365
caa aag gcg gga gag aaa gga gag gct tca aca gag gct gag cca atg 1152
Gln Lys Ala Gly Glu Lys Gly Glu Ala Ser Thr Glu Ala Glu Pro Met
370 375 380
gac gca agt agt aat gct caa gaa tga 1179
Asp Ala Ser Ser Asn Ala Gln Glu
385 390
<210>4
<211>392
<212>PRT
<213>Arabidopsis thaliana
<400>4
Met Ser Ser Asp Asp Glu Arg Asp Glu Lys Glu Leu Ser Leu Thr Ser
1 5 10 15
Pro Glu Val Val Thr Lys Tyr Lys Ser Ala Ala Glu Ile Val Asn Lys
20 25 30
Ala Leu Gln Val Val Leu Ala Glu Cys Lys Pro Lys Ala Lys Ile Val
35 40 45
Asp Ile Cys Glu Lys Gly Asp Ser Phe Ile Lys Glu Gln rhr Ala Ser
50 55 60
Met Tyr Lys Asn Ser Lys Lys Lys Ile Glu Arg Gly Val Ala Phe Pro
CCR专利
65 70 75 80
Thr Cys Ile Ser Val Asn Asn Thr Val Gly His Phe Ser Pro Leu Ala
85 90 95
Ser Asp Glu Ser Val Leu Glu Asp Gly Asp Met Val Lys Ile Asp Met
100 105 110
Gly Cys His Ile Asp Gly Phe Ile Ala Leu Val Gly His Thr His Val
115 120 125
Leu Gln Glu Gly Pro Leu Ser Gly Arg Lys Ala Asp Val Ile Ala Ala
130 135 140
Ala Asn Thr Ala Ala Asp Val Ala Leu Arg Leu Val Arg Pro Gly Lys
145 150 155 160
Lys Asn Thr Asp Val Thr Glu Ala Ile Gln Lys Val Ala Ala Ala Tyr
165 170 175
Asp Cys Lys Ile Val Glu Gly Val Leu Ser His Gln Leu Lys Gln His
180 185 190
Val Ile Asp Gly Asn Lys Val Val Leu Ser Val Ser Ser Pro Glu Thr
195 200 205
Thr Val Asp Glu Val Glu Phe Glu Glu Asn Glu Val Tyr Ala Ile Asp
210 215 220
Ile Val Ala Ser Thr Gly Asp Gly Lys Pro Lys Leu Leu Asp Glu Lys
225 230 235 240
Gln Thr Thr Ile Tyr Lys Lys Asp Glu Ser Val Asn Tyr Gln Leu Lys
245 250 255
Met Lys Ala Ser Arg Phe Ile Ile Ser Glu Ile Lys Gln Asn Phe Pro
260 265 270
Arg Met Pro Phe Thr Ala Arg Ser Leu Glu Glu Lys Arg Ala Arg Leu
275 280 285
Gly Leu Val Glu Cys Val Asn His Gly His Leu Gln Pro Tyr Pro Val
290 295 300
Leu Tyr Glu Lys Pro Gly Asp Phe Val Ala Gln Ile Lys Phe Thr Val
305 310 315 320
Leu Leu Met Pro Asn Gly Ser Asp Arg Ile Thr Ser His Thr Leu Gln
325 330 335
Glu Leu Pro Lys Lys Thr Ile Glu Asp Pro Glu Ile Lys Gly Trp Leu
340 345 350
Ala Leu Gly Ile Lys Lys Lys Lys Gly Gly Gly Lys Lys Lys Lys Ala
355 360 365
CCR专利
Gln Lys Ala Gly Glu Lys Gly Glu Ala Ser Thr Glu Ala Glu Pro Met
370 375 380
Asp Ala Ser Ser Asn Ala Gln Glu
385 390
<210>5
<211>1182
<212>DNA
<213>Oryza sativa
<220>
<221>CDS
<222>(1)..(1179)
<223>
<400>5
atg tcg tcg gac gat gag gtc aga gag gag aag gag ctc gac ctc tcc 48
Met Ser Ser Asp Asp Glu Val Arg Glu Glu Lys Glu Leu Asp Leu Ser
1 5 10 15
tcc tcc gac gtc gtc acc aag tac aag gac gcc gcc gat atc atc aac 96
Ser Ser Asp Val Val Thr Lys Tyr Lys Asp Ala Ala Asp Ile Ile Asn
20 25 30
aat gct ctt aag ttg gta gtg tct ttg tgc aag ccg aaa gcc aag att 144
Asn Ala Leu Lys Leu Val Val Ser Leu Cys Lys Pro Lys Ala Lys Ile
35 40 45
gtt gac atc tgt gag aag ggc gat tct tac ata aga gag caa act ggc 192
Val Asp lle Cys Glu Lys Gly Asp Ser Tyr Ile Arg Glu Gln Thr Gly
50 55 60
aac atc tac aag aat gtg aag agg aag att gaa aga ggt gtt gct ttt 240
Asn Ile Tyr Lys Asn Val Lys Arg Lys Ile Glu Arg Gly Val Ala Phe
65 70 75 80
cca aca tgt gta tct gtg aac aac aca gtc tgc cat ttc tct ccg cta 288
Pro Thr Cys Val Ser Val Asn Asn Thr Val Cys His Phe Ser Pro Leu
85 90 95
gcc act gat gag gca gtc ctg gag gaa aat gat atg gtc aaa att gat 336
Ala Thr Asp Glu Ala Val Leu Glu Glu Asn Asp Met Val Lys Ile Asp
100 105 110
atg gga tgt cat att gat ggt ttt att gcc gtg gtg gct cat aca cat 384
Met Gly Cys His Ile Asp Gly Phe Ile Ala Val Val Ala His Thr His
115 120 125
gtt atc cat gat gga gca gtc act ggg aaa gca gca gat gtt ctt gct 432
Val Ile His Asp Gly Ala Val Thr Gly Lys Ala Ala Asp Val Leu Ala
130 135 140
gct gcc aac aca gcg gca gaa gtt gct ctg agg ttt gtt aga cct ggg 480
Ala Ala Asn Thr Ala Ala Glu Val Ala Leu Arg Phe Val Arg Pro Gly
145 150 155 160
aag aag aac aag gat gtc acc gaa gcg att cag aaa gtt gct gct gcc 528
Lys Lys Asn Lys Asp Val Thr Glu Ala Ile Gln Lys Val Ala Ala Ala
165 170 175
tat gac tcc gta tct gtt tga gga gtt ctc agc cat cag ctg aaa cag 576
Tyr Asp Ser Val Ser Val Glu Gly Val Leu Ser His Gln Leu Lys Gln
180 185 190
ttt gtc att gac ggt aac aaa gtt gta ctt agt gtg tcc aat gct gat 624
Phe Val Ile Asp Gly Asn Lys Val Val Leu Ser Val Ser Asn Ala Asp
195 200 205
acg aag gtg gat gat gct gaa ttt gag gaa aat gaa gtg tat gca att 672
Thr Lys Val Asp Asp Ala Glu Phe Glu Glu Asn Glu Val Tyr Ala Ile
210 215 220
CCR专利
gat att gtc acc agc act gga gaa ggg aag ccc aag tta ttg gat gag 720
Asp Ile Val Thr Ser Thr Gly Glu Gly Lys Pro Lys Leu Leu Asp Glu
225 230 235 240
aag cag acc acc atc tac aag aga gct gta gac aag aat tat cac ttg 768
Lys Gln Thr Thr Ile Tyr Lys Arg Ala Val Asp Lys Asn Tyr His Leu
245 250 255
aag atg aaa gca tct agg ttc att ttc agt gag ata agc cag aag ttt 816
Lys Met Lys Ala Ser Arg Phe Ile Phe Ser Glu Ile Ser Gln Lys Phe
260 265 270
cct atc atg ccc ttc act gct agg gca ttg gaa gag aag cgt gca cgc 864
Pro Ile Met Pro Phe Thr Ala Arg Ala Leu Glu Glu Lys Arg Ala Arg
275 280 285
tta ggt ttg gtg gaa tgc atg aat cat gaa tta ttg cag ccg tac cct 912
Leu Gly Leu Val Glu Cys Met Asn His Glu Leu Leu Gln Pro Tyr Pro
290 295 300
gtc ctt cac gag aag cca ggc gac crt gtt gca cac atc aag ttt act 960
Val Leu His Glu Lys Pro Gly Asp Leu Val Ala His Ile Lys Phe Thr
305 310 315 320
gtg ctt ctg ctg cca tct ggt tca cag agg aca aca tcg cat tca ctt 1008
Val Leu Leu Leu Pro Ser Gly Ser Gln Arg Val Thr Ser His Ser Leu
325 330 335
cag gtg ttg cag ccc act aaa tcc att gaa gac cac gct gag atc aag 1056
Gln Val Leu Gln Pro Thr Lys Ser Ile Glu Asp His Ala Glu Ile Lys
340 345 350
gca tgg ctt gct ttg ggg acc aaa aca aag aag aaa agt ggt gcc caa 1104
Ala Trp Leu Ala Leu Gly Thr Lys Thr Lys Lys Lys Ser Gly Ala Gln
355 360 365
aag aag aaa ggt aag aaa gga gat gca gca gaa gca gtg cct atg gag 1152
Lys Lys Lys Gly Lys Lys Gly Asp Ala Ala Glu Ala Val Pro Met Glu
370 375 380
gag ggt tca aat gat gca aac aaa gaa taa 1182
Glu Gly Ser Asn Asp Ala Asn Lys Glu
385 390
<210>6
<211>393
<212>PRT
<213>Oryza sativa
<400>6
Met Ser Ser Asp Asp Glu Val Arg Glu Glu Lys Glu Leu Asp Leu Ser
1 5 10 15
Ser Ser Asp Val Val Thr Lys Tyr Lys Asp Ala Ala Asp Ile Ile Asn
20 25 30
Asn Ala Leu Lys Leu Val Val Ser Leu Cys Lys Pro Lys Ala Lys Ile
35 40 45
Val Asp Ile Cys Glu Lys Gly Asp Ser Tyr Ile Arg Glu Gln Thr Gly
50 55 60
Asn Ile Tyr Lys Asn Val Lys Arg Lys Ile Glu Arg Gly Val Ala Phe
65 70 75 80
Pro Thr Cys Val Ser Val Asn Asn Thr Val Cys His Phe Ser Pro Leu
85 90 95
CCR专利
Ala Thr Asp Glu Ala Val Leu Glu Glu Asn Asp Met Val Lys Ile Asp
100 105 110
Met Gly Cys His Ile Asp Gly Phe Ile Ala Val Val Ala His Thr His
115 120 125
Val Ile His Asp Gly Ala Val Thr Gly Lys Ala Ala Asp Val Leu Ala
130 135 140
Ala Ala Asn Thr Ala Ala Glu Val Ala Leu Arg Phe Val Arg Pro Gly
145 150 155 160
Lys Lys Asn Lys Asp Val Thr Glu Ala Ile Gln Lys Val Ala Ala Ala
165 170 175
Tyr Asp Ser Val Ser Val Glu Gly Val Leu Ser His Gln Leu Lys Gln
180 185 190
Phe Val Ile Asp Gly Asn Lys Val Val Leu Ser Val Ser Asn Ala Asp
195 200 205
Thr Lys Val Asp Asp Ala Glu Phe Glu Glu Asn Glu Val Tyr Ala Ile
210 215 220
Asp Ile Val Thr Ser Thr Gly Glu Gly Lys Pro Lys Leu Asp Glu
225 230 235 240
Lys Gln Thr Thr Ile Tyr Lys Arg Ala Val Asp Lys Asn Tyr His Leu
245 250 255
Lys Met Lys Ala Ser Arg Phe Ile Phe Ser Glu Ile Ser Gln Lys Phe
260 265 270
Pro Ile Met Pro Phe Thr Ala Arg Ala Leu Glu Glu Lys Arg Ala Arg
275 280 285
Leu Gly Leu Val Glu Cys Met Asn His Glu Leu Leu Gln Pro Tyr Pro
290 295 300
Val Leu His Glu Lys Pro Gly Asp Leu Val Ala His Ile Lys Phe Thr
305 310 315 320
Val Leu Leu Leu Pro Ser Gly Ser Gln Arg Val Thr Ser His Ser Leu
325 330 335
Gln Val Leu Gln Pro Thr Lys Ser Ile Glu Asp His Ala Glu Ile Lys
340 345 350
Ala Trp Leu Ala Leu Gly Thr Lys Thr Lys Lys Lys Ser Gly Ala Gln
355 360 365
Lys Lys Lys Gly Lys Lys Gly Asp Ala Ala Glu Ala Val Pro Met Glu
370 375 380
Glu Gly Ser Asn Asp Ala Asn Lys Glu
385 390
Claims (4)
1.一种存在于植物中的参予细胞分裂G1期至S期与细胞周期相关的基因CCR所编码的蛋白质，它来自拟南芥(Arabidopsis thaliana)，命名为AtCCR的蛋白质，它由如下所示的氨基酸序列组成：
MSSDDERDEKELSLTSPEVVTKYKSAAEIVNKALQVVLAECKPKAKIVDICEKGDS
FIKEQTASMYKNSKKKIERGVAFPTCISVNNTVGHFSPLASDESVLEDGDMVKIDM
GCHIDGFIALVGHTHVLQEGPLSGRKADVIAAANTAADVALRLVRPGKKNTDVTEA
IQKVAAAYDCKIVEGVLSHQLKQHVIDGNKVVLSVSSPETTVDEVEFEENEVYAIDI
VASTGDGKPKLLDEKQTTIYKKDESVNYQLKMKASRFIISEIKQNFPRMPFTARSLE
EKRARLGLVECVNHGHLQPYPVLYEKPGDFVAQIKFTVLLMPNGSDRITSHTLQELP
KKTIEDPEIKGWLALGIKKKKGGGKKKKAQKAGEKGEASTEAEPMDASSNAQE。
2.一种编码权利要求1所述的蛋白质的基因，它由如下所示的核苷酸序列组成：
CATTTCTCTCCTTCTCCACCAGTTTTCCCCCAATCGTTCCTCCTTAAACCCTAACA
TTTTTGCTTCCCCTGTTTCGATTCTCGAGAGTTATCAGAGATGAGTTCGGACGAT
GAGAGAGACGAGAAGGAGCTGAGTCTTACCTCTCCTGAAGTCGTCACCAAGTA
CAAGAGCGCCGCTGAGATCGTTAACAAGGCGTTACAGGTTGTTTTAGCTGAATG
CAAACCAAAAGCTAAGATTGTTGATATCTGTGAGAAAGGAGACTCTTTTATTAAA
GAGCAAACAGCAAGCATGTACAAGAATTCTAAGAAGAAGATTGAGAGAGGTGT
TGCGTTCCCTACATGCATTTCTGTGAACAACACTGTTGGTCATTTTTCACCGCTTG
CTAGTGATGAGTCTGTGTTGGAAGATGGTGACATGGTTAAAATCGATATGGGATG
TCATATTGATGGGTTCATTGCCCTTGTTGGTCACACACATGTTCTTCAAGAAGGG
CCTCTTATAAGGCTGATGTTATCGCTGCCGCAAATACTGCAGCTGATGTTGCTCTA
AGGCTCGTACGTCCTGGAAAAAAGAACACTGATGTAACTGAAGCTATTCAGAAG
GTAGCTGCAGCTTATGACTGCAAAATTGTTGAAGGTGTTCTTTCCCACCAGCTGA
AACAGCATGTGATAGATGGAAACAAGGTTGTGCTTAGTGTATCCAGCCCTGAAA
CAACTGTTGACGAAGTGGAATTTGAAGAGAATGAAGTCTATGCAATAGATATTGT
GGCAAGTACTGGTGATGGCAAGCCAAAGCTATTAGACGAGAAGCAAACAACTAT
TTACAAGAAAGATGAAAGTGTTAACTATCAGTTGAAGATGAAGGCCTCCAGATT
CATAATCAGCGAAATTAAACAGAACTTCCCCCGTATGCCATTCACTGCAAGGTCA
CTGGAGGAGAAAAGGGCACGGCTATTTCTTTGCTTGTCCTAATCTTATCTCATTCT
CT。
3.根据权利要求1所述的蛋白质在培育和改良植物品种中的应用。
4.根据权利要求2所述的基因在培育和改良植物品种中的应用。
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 02108797 CN1227262C (zh) | 2002-04-02 | 2002-04-02 | 一种与植物细胞周期相关的蛋白质和其编码基因 |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 02108797 CN1227262C (zh) | 2002-04-02 | 2002-04-02 | 一种与植物细胞周期相关的蛋白质和其编码基因 |
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| CN115443908B (zh) * | 2022-01-04 | 2023-09-26 | 北京林业大学 | 一种提高植物遗传转化效率的方法 |
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