CN120700119A - 用于减少非特异性扩增产物的方法和组合物 - Google Patents
用于减少非特异性扩增产物的方法和组合物Info
- Publication number
- CN120700119A CN120700119A CN202510844889.XA CN202510844889A CN120700119A CN 120700119 A CN120700119 A CN 120700119A CN 202510844889 A CN202510844889 A CN 202510844889A CN 120700119 A CN120700119 A CN 120700119A
- Authority
- CN
- China
- Prior art keywords
- dna
- target
- amplification products
- specific amplification
- specific
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6846—Common amplification features
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2521/00—Reaction characterised by the enzymatic activity
- C12Q2521/30—Phosphoric diester hydrolysing, i.e. nuclease
- C12Q2521/301—Endonuclease
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
- C12Q2525/10—Modifications characterised by
- C12Q2525/191—Modifications characterised by incorporating an adaptor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2527/00—Reactions demanding special reaction conditions
- C12Q2527/101—Temperature
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2537/00—Reactions characterised by the reaction format or use of a specific feature
- C12Q2537/10—Reactions characterised by the reaction format or use of a specific feature the purpose or use of
- C12Q2537/143—Multiplexing, i.e. use of multiple primers or probes in a single reaction, usually for simultaneously analyse of multiple analysis
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Analytical Chemistry (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Immunology (AREA)
- Biotechnology (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
Applications Claiming Priority (6)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201562114788P | 2015-02-11 | 2015-02-11 | |
| US62/114,788 | 2015-02-11 | ||
| US201562150600P | 2015-04-21 | 2015-04-21 | |
| US62/150,600 | 2015-04-21 | ||
| PCT/US2016/017454 WO2016130746A1 (en) | 2015-02-11 | 2016-02-11 | Method and compositions for reducing non-specific amplification products |
| CN201680021382.3A CN107532166A (zh) | 2015-02-11 | 2016-02-11 | 用于减少非特异性扩增产物的方法和组合物 |
Related Parent Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201680021382.3A Division CN107532166A (zh) | 2015-02-11 | 2016-02-11 | 用于减少非特异性扩增产物的方法和组合物 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN120700119A true CN120700119A (zh) | 2025-09-26 |
Family
ID=56566583
Family Applications (3)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202510844889.XA Pending CN120700119A (zh) | 2015-02-11 | 2016-02-11 | 用于减少非特异性扩增产物的方法和组合物 |
| CN201680021382.3A Pending CN107532166A (zh) | 2015-02-11 | 2016-02-11 | 用于减少非特异性扩增产物的方法和组合物 |
| CN202510844827.9A Pending CN120700118A (zh) | 2015-02-11 | 2016-02-11 | 用于减少非特异性扩增产物的方法和组合物 |
Family Applications After (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201680021382.3A Pending CN107532166A (zh) | 2015-02-11 | 2016-02-11 | 用于减少非特异性扩增产物的方法和组合物 |
| CN202510844827.9A Pending CN120700118A (zh) | 2015-02-11 | 2016-02-11 | 用于减少非特异性扩增产物的方法和组合物 |
Country Status (10)
| Country | Link |
|---|---|
| US (1) | US9464318B2 (https=) |
| EP (1) | EP3256584B1 (https=) |
| JP (1) | JP6755890B2 (https=) |
| KR (1) | KR20170110721A (https=) |
| CN (3) | CN120700119A (https=) |
| AU (1) | AU2016219288B2 (https=) |
| BR (1) | BR112017017142B1 (https=) |
| CA (1) | CA2976251C (https=) |
| ES (1) | ES2795404T3 (https=) |
| WO (1) | WO2016130746A1 (https=) |
Families Citing this family (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US10421993B2 (en) | 2015-02-11 | 2019-09-24 | Paragon Genomics, Inc. | Methods and compositions for reducing non-specific amplification products |
| CN107058573B (zh) * | 2016-06-13 | 2020-07-10 | 艾吉泰康生物科技(北京)有限公司 | 一种利用Cas9/gRNA系统构建扩增子文库的方法 |
| CN110382716A (zh) * | 2017-01-10 | 2019-10-25 | 佰隆基因公司 | 用于减少引物延伸反应中产生的冗余分子条形码的方法和组合物 |
| WO2018140329A1 (en) * | 2017-01-24 | 2018-08-02 | Tsavachidou Dimitra | Methods for constructing copies of nucleic acid molecules |
| US11898204B2 (en) * | 2018-03-02 | 2024-02-13 | Roche Sequencing Solutions, Inc. | Generation of single-stranded circular DNA templates for single molecule sequencing |
| US11976275B2 (en) | 2018-06-15 | 2024-05-07 | Kapa Biosystems, Inc. | Generation of double-stranded DNA templates for single molecule sequencing |
| JP7626430B2 (ja) * | 2019-01-09 | 2025-02-04 | 国立研究開発法人理化学研究所 | 薬物動態関連遺伝子の網羅的配列解析法とそれに使用されるプライマーセット |
| US11396674B2 (en) * | 2019-02-22 | 2022-07-26 | Gianluca ROMA | Multiplex nucleic acid amplification and library preparation |
| US10941453B1 (en) | 2020-05-20 | 2021-03-09 | Paragon Genomics, Inc. | High throughput detection of pathogen RNA in clinical specimens |
| CN114875119B (zh) * | 2021-02-05 | 2025-11-18 | 广东菲鹏生物有限公司 | 一种提高核酸扩增产物浓度的方法及其应用 |
| CN114369649A (zh) * | 2022-02-08 | 2022-04-19 | 山东见微生物科技有限公司 | 一种特异的选择扩增及多重pcr方法和应用 |
| US12091715B2 (en) | 2022-04-21 | 2024-09-17 | Paragon Genomics, Inc. | Methods and compositions for reducing base errors of massive parallel sequencing using triseq sequencing |
| US11680293B1 (en) | 2022-04-21 | 2023-06-20 | Paragon Genomics, Inc. | Methods and compositions for amplifying DNA and generating DNA sequencing results from target-enriched DNA molecules |
| CN114774526B (zh) * | 2022-04-25 | 2025-12-12 | 陕西科技大学 | 一种dna长度的熔解测定方法及试剂盒 |
| CN121183047B (zh) * | 2025-11-24 | 2026-03-31 | 湖南圣测生物科技有限公司 | 一种同时检测非洲猪瘟病毒、猪流行性腹泻病毒、猪繁殖与呼吸综合征病毒的引物探针组合、试剂盒及其应用 |
Family Cites Families (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5750341A (en) | 1995-04-17 | 1998-05-12 | Lynx Therapeutics, Inc. | DNA sequencing by parallel oligonucleotide extensions |
| US20040002156A1 (en) * | 2002-06-26 | 2004-01-01 | Stratagene | Selective cloning of homoduplex nucleic acids |
| WO2005003375A2 (en) * | 2003-01-29 | 2005-01-13 | 454 Corporation | Methods of amplifying and sequencing nucleic acids |
| EP1574570A1 (en) * | 2004-03-12 | 2005-09-14 | Universität Regensburg | Process for reducing the number of mismatches in double stranded polynucleotides |
| CN101124337B (zh) | 2004-08-24 | 2010-11-17 | 康乃尔研究基金会有限公司 | 使用内切核酸酶剪切/连接酶重新密封反应和毛细管电泳或微阵列检测核酸差异 |
| WO2006127423A2 (en) * | 2005-05-18 | 2006-11-30 | Codon Devices, Inc. | Methods of producing polynucleotide libraries using scarless ligation |
| US8716190B2 (en) * | 2007-09-14 | 2014-05-06 | Affymetrix, Inc. | Amplification and analysis of selected targets on solid supports |
| WO2012044847A1 (en) | 2010-10-01 | 2012-04-05 | Life Technologies Corporation | Nucleic acid adaptors and uses thereof |
| US8586310B2 (en) | 2008-09-05 | 2013-11-19 | Washington University | Method for multiplexed nucleic acid patch polymerase chain reaction |
| US9957558B2 (en) | 2011-04-28 | 2018-05-01 | Life Technologies Corporation | Methods and compositions for multiplex PCR |
| EP2943590A4 (en) | 2013-01-13 | 2017-02-15 | Unitaq Bio | Methods and compositions for pcr using blocked and universal primers |
| WO2015063154A1 (en) | 2013-10-31 | 2015-05-07 | Multiplicom N.V. | Blocking primers in multiplex pcr based assays |
-
2016
- 2016-02-11 EP EP16749846.8A patent/EP3256584B1/en active Active
- 2016-02-11 WO PCT/US2016/017454 patent/WO2016130746A1/en not_active Ceased
- 2016-02-11 KR KR1020177025384A patent/KR20170110721A/ko not_active Ceased
- 2016-02-11 ES ES16749846T patent/ES2795404T3/es active Active
- 2016-02-11 CN CN202510844889.XA patent/CN120700119A/zh active Pending
- 2016-02-11 CN CN201680021382.3A patent/CN107532166A/zh active Pending
- 2016-02-11 US US15/041,644 patent/US9464318B2/en active Active
- 2016-02-11 CN CN202510844827.9A patent/CN120700118A/zh active Pending
- 2016-02-11 CA CA2976251A patent/CA2976251C/en active Active
- 2016-02-11 BR BR112017017142-2A patent/BR112017017142B1/pt active IP Right Grant
- 2016-02-11 AU AU2016219288A patent/AU2016219288B2/en active Active
- 2016-02-11 JP JP2017560886A patent/JP6755890B2/ja active Active
Also Published As
| Publication number | Publication date |
|---|---|
| JP2018506307A (ja) | 2018-03-08 |
| EP3256584A1 (en) | 2017-12-20 |
| CN120700118A (zh) | 2025-09-26 |
| BR112017017142A2 (en) | 2018-04-03 |
| US9464318B2 (en) | 2016-10-11 |
| KR20170110721A (ko) | 2017-10-11 |
| US20160230222A1 (en) | 2016-08-11 |
| CA2976251A1 (en) | 2016-08-18 |
| BR112017017142B1 (pt) | 2024-03-12 |
| EP3256584B1 (en) | 2020-03-25 |
| EP3256584A4 (en) | 2018-08-15 |
| ES2795404T3 (es) | 2020-11-23 |
| CA2976251C (en) | 2023-08-22 |
| CN107532166A (zh) | 2018-01-02 |
| AU2016219288A1 (en) | 2017-08-17 |
| AU2016219288B2 (en) | 2021-10-28 |
| JP6755890B2 (ja) | 2020-09-16 |
| WO2016130746A1 (en) | 2016-08-18 |
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Legal Events
| Date | Code | Title | Description |
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| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |