CN118931711A - 单通道测序装置和测序方法 - Google Patents
单通道测序装置和测序方法 Download PDFInfo
- Publication number
- CN118931711A CN118931711A CN202410524599.2A CN202410524599A CN118931711A CN 118931711 A CN118931711 A CN 118931711A CN 202410524599 A CN202410524599 A CN 202410524599A CN 118931711 A CN118931711 A CN 118931711A
- Authority
- CN
- China
- Prior art keywords
- sequencing
- nucleotide
- nucleic acid
- molecular marker
- reaction
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
- C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202410524599.2A CN118931711A (zh) | 2019-05-15 | 2019-05-15 | 单通道测序装置和测序方法 |
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PCT/CN2019/086974 WO2020227953A1 (zh) | 2019-05-15 | 2019-05-15 | 一种基于自发光的单通道测序方法 |
| CN201980095781.8A CN113748216B (zh) | 2019-05-15 | 2019-05-15 | 一种基于自发光的单通道测序方法 |
| CN202410524599.2A CN118931711A (zh) | 2019-05-15 | 2019-05-15 | 单通道测序装置和测序方法 |
Related Parent Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201980095781.8A Division CN113748216B (zh) | 2019-05-15 | 2019-05-15 | 一种基于自发光的单通道测序方法 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN118931711A true CN118931711A (zh) | 2024-11-12 |
Family
ID=73289989
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202410524599.2A Pending CN118931711A (zh) | 2019-05-15 | 2019-05-15 | 单通道测序装置和测序方法 |
| CN201980095781.8A Active CN113748216B (zh) | 2019-05-15 | 2019-05-15 | 一种基于自发光的单通道测序方法 |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201980095781.8A Active CN113748216B (zh) | 2019-05-15 | 2019-05-15 | 一种基于自发光的单通道测序方法 |
Country Status (12)
| Country | Link |
|---|---|
| US (1) | US12565677B2 (enExample) |
| EP (1) | EP3988670B1 (enExample) |
| JP (2) | JP7485483B2 (enExample) |
| KR (1) | KR102843262B1 (enExample) |
| CN (2) | CN118931711A (enExample) |
| AU (1) | AU2019445584B2 (enExample) |
| BR (1) | BR112021022809A2 (enExample) |
| CA (1) | CA3139169A1 (enExample) |
| MX (1) | MX2021013983A (enExample) |
| SG (1) | SG11202111778YA (enExample) |
| WO (1) | WO2020227953A1 (enExample) |
| ZA (1) | ZA202110159B (enExample) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114286867B (zh) * | 2019-08-20 | 2024-04-16 | 青岛华大智造科技有限责任公司 | 一种基于发光标记物光信号动力学及二次发光信号对多核苷酸进行测序的方法 |
| IL315955A (en) | 2022-03-30 | 2024-11-01 | Peptidream Inc | Peptide complex having trkb binding activity |
| CN115232867B (zh) * | 2022-07-21 | 2024-01-30 | 深圳赛陆医疗科技有限公司 | 一种快速的二代基因测序方法 |
| JP2025531816A (ja) | 2022-09-09 | 2025-09-25 | ビージーアイ シェンチェン | 核酸検出におけるヘテロ芳香環化合物の使用 |
| US20250297306A1 (en) | 2022-09-28 | 2025-09-25 | Mgi Tech Co., Ltd. | Sequencing method |
| WO2024098185A1 (en) * | 2022-11-07 | 2024-05-16 | GeneSense Technology Inc., Shanghai (CN) | Nucleic acid sequencing using self-luminescence |
| EP4592403A4 (en) | 2022-11-22 | 2025-11-26 | Mgi Tech Co Ltd | GENE SEQUENCING CHIP, PACKAGING STRUCTURE, SYSTEM AND PROCESSES FOR CLEANING, PRODUCTION AND SEQUENCING |
Family Cites Families (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1991006678A1 (en) | 1989-10-26 | 1991-05-16 | Sri International | Dna sequencing |
| WO2000006770A1 (en) | 1998-07-30 | 2000-02-10 | Solexa Ltd. | Arrayed biomolecules and their use in sequencing |
| AU2001296645A1 (en) | 2000-10-06 | 2002-04-15 | The Trustees Of Columbia University In The City Of New York | Massive parallel method for decoding dna and rna |
| WO2004018497A2 (en) | 2002-08-23 | 2004-03-04 | Solexa Limited | Modified nucleotides for polynucleotide sequencing |
| EP1701785A1 (en) | 2004-01-07 | 2006-09-20 | Solexa Ltd. | Modified molecular arrays |
| GB0427236D0 (en) | 2004-12-13 | 2005-01-12 | Solexa Ltd | Improved method of nucleotide detection |
| CN1854308A (zh) * | 2005-04-21 | 2006-11-01 | 株式会社日立制作所 | 碱基序列检测方法 |
| CA2859660C (en) * | 2011-09-23 | 2021-02-09 | Illumina, Inc. | Methods and compositions for nucleic acid sequencing |
| WO2014139596A1 (en) | 2013-03-15 | 2014-09-18 | Illumina Cambridge Limited | Modified nucleosides or nucleotides |
| ES2803079T3 (es) * | 2014-09-12 | 2021-01-22 | Illumina Inc | Métodos para detectar la presencia de subunidades de polímeros usando quimioluminiscencia |
| EP3411494B1 (en) * | 2015-11-18 | 2025-08-13 | Kalim U. Mir | Super-resolution sequencing |
| CN113321943A (zh) * | 2015-11-20 | 2021-08-31 | 加利福尼亚太平洋生物科学股份有限公司 | 标记的核苷酸类似物、反应混合物以及测序方法和系统 |
| RU2760737C2 (ru) | 2016-12-27 | 2021-11-30 | Еги Тек (Шэнь Чжэнь) Ко., Лимитед | Способ секвенирования на основе одного флуоресцентного красителя |
| ES3026859T3 (en) | 2017-01-04 | 2025-06-12 | Mgi Tech Co Ltd | Stepwise sequencing by non-labeled reversible terminators or natural nucleotides |
| BR112019014683A2 (pt) | 2017-03-07 | 2020-02-18 | Illumina, Inc. | Sequenciamento com dois canais óticos, com fonte de luz única |
| WO2019071474A1 (zh) * | 2017-10-11 | 2019-04-18 | 深圳华大智造科技有限公司 | 修饰的核苷或核苷酸 |
-
2019
- 2019-05-15 BR BR112021022809A patent/BR112021022809A2/pt unknown
- 2019-05-15 CN CN202410524599.2A patent/CN118931711A/zh active Pending
- 2019-05-15 SG SG11202111778YA patent/SG11202111778YA/en unknown
- 2019-05-15 JP JP2021563644A patent/JP7485483B2/ja active Active
- 2019-05-15 US US17/608,316 patent/US12565677B2/en active Active
- 2019-05-15 AU AU2019445584A patent/AU2019445584B2/en active Active
- 2019-05-15 EP EP19928475.3A patent/EP3988670B1/en active Active
- 2019-05-15 KR KR1020217040845A patent/KR102843262B1/ko active Active
- 2019-05-15 CA CA3139169A patent/CA3139169A1/en active Pending
- 2019-05-15 WO PCT/CN2019/086974 patent/WO2020227953A1/zh not_active Ceased
- 2019-05-15 MX MX2021013983A patent/MX2021013983A/es unknown
- 2019-05-15 CN CN201980095781.8A patent/CN113748216B/zh active Active
-
2021
- 2021-12-08 ZA ZA2021/10159A patent/ZA202110159B/en unknown
-
2024
- 2024-04-30 JP JP2024073905A patent/JP7825660B2/ja active Active
Also Published As
| Publication number | Publication date |
|---|---|
| JP2022533916A (ja) | 2022-07-27 |
| EP3988670A1 (en) | 2022-04-27 |
| CN113748216B (zh) | 2024-04-23 |
| SG11202111778YA (en) | 2021-12-30 |
| KR102843262B1 (ko) | 2025-08-06 |
| KR20220009992A (ko) | 2022-01-25 |
| AU2019445584B2 (en) | 2024-09-05 |
| JP2024099789A (ja) | 2024-07-25 |
| CN113748216A (zh) | 2021-12-03 |
| EP3988670A4 (en) | 2023-01-25 |
| BR112021022809A2 (pt) | 2022-01-25 |
| MX2021013983A (es) | 2022-02-10 |
| JP7485483B2 (ja) | 2024-05-16 |
| EP3988670B1 (en) | 2026-04-22 |
| JP7825660B2 (ja) | 2026-03-06 |
| AU2019445584A1 (en) | 2022-01-20 |
| US20220205036A1 (en) | 2022-06-30 |
| CA3139169A1 (en) | 2020-11-19 |
| ZA202110159B (en) | 2022-08-31 |
| US12565677B2 (en) | 2026-03-03 |
| WO2020227953A1 (zh) | 2020-11-19 |
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| SE01 | Entry into force of request for substantive examination |