CN1186680A - High effect nontoxic natural anti-cancer agent - Google Patents

Abstract

The said medicine is powder obtained through cell wall breaking extraction of the fruit of Chinese fan palm, one kind of palm plant. It has the special function of preventing and curing cancer and can raise immunity of organism without toxic side effect. The said medicine is suitable for production in various producer of health article of Chinese medicine.

Description

High effect nontoxic natural anti-cancer agent

Semen livistonae chinensis is a kind of wild or tame aiphyllium, belongs to Petiolus Trachycarpi (Trachycar PusFortunei) section Semen livistonae chinensis (Liuistona Chinensis), also claims " flabellum certain herbaceous plants with big flowers ".It originates in south China, and Fujian, Guangdong, Guangxi one band are cultivated more.Also there is distribution in Vietnam.Through studying for a long period of time and clinical discovery, the former powder of Semen livistonae chinensis that the Semen livistonae chinensis fruit is extracted into high-tech approach (the former powder of LCP) has prevention and treats the anticancer preparation of the auxotype of all kinds of cancers, also is a kind of efficient, nontoxic more satisfactory cancer therapy drug.

Natural anti-cancer agent of the present invention (be called for short LCP) relates in health product and the field of medicaments prophylaxis of cancer, treats important breakthrough and the former powder extraction of effective components of LCP on the cancer.

Common both at home and abroad prevention and treatment method for cancer, nothing more than early stage inspection, early discovery, early diagnosis, usually adopt means such as surgical operation and Drug therapy and chemotherapy, radiotherapy, laser therapy, though these Therapeutic Method and means can obtain effect, but toxic and side effects is bigger, and healing or survival rate are all lower.

The present invention from the Semen livistonae chinensis fruit through pulverize with various breaking cell wall technical finesses after, the various preparations that the effective ingredient of the former powder of reuse biotechnology extraction LCP is made, clinical 120 many cases such as medium and advanced lung cancer, hepatocarcinoma, renal carcinoma, breast carcinoma, cervical cancer, nasopharyngeal carcinoma, laryngeal carcinoma, squamous cell carcinoma and other tumors that have been used for the treatment of, all obtained good curative effect, make to threaten the healing of human incurable disease dawn to occur, thereby the survival rate of cancer, cure rate are also increased substantially.LCP of the present invention is not only evident in efficacy to above-mentioned serious symptom, and rich in proteins, polysaccharide and contain 16 kinds of trace element such as zinc, chromium, copper, manganese, magnesium.But the normal person takes also human body immunity improving function.

Now extract the method for LCP from Semen livistonae chinensis, the zoopery of the acute toxicity testing of LCP, experiment in vitro, vivo antitumor effect, immunology observation experiment, clinical model case are specified in the back respectively:

One, extract the method for LCP: with reference to Chinese application number is the method for 95117499.1,95118411.8 patent breaking cell walls.

Embodiment 1. is by Fig. 1. and production technology is operated, with clean the drying of 500g Semen livistonae chinensis fruit (band shell or shelling), be crushed to grain of rice size with pulverizer and soak 8 hours (being shown in Table 1) with 98-50% each 400ml medicinal alcohol respectively, behind the reclaim under reduced pressure ethanol, residue filters, filtrate keeps, and residue extracts with distilled water 500ml, filters, filtrate keeps, water is proposed operation repeat 2 times, merging filtrate, steaming dewaters, when being thick, place 40-50 ℃ of oven dry of far-infrared oven again, pulverized 60 mesh sieves, productive rate is 4.5-4.9% as shown in table 1.Table 1

Alcohol concentration %	??98	??90	??80	??70	??60	??50
							Ethanol volume (ml)	??400	??400	??400	??400	??400	??400
LCP yield %	??4.5	??4.6	??4.6	??4.8	??4.85	??4.9

Embodiment 2, press Fig. 2, production technology operation: the 500g Semen livistonae chinensis is really blended be grain of rice size, add 1.2 liters of distilled waters, 5 minutes after-filtration of heated and boiled repeat aforesaid operations 11 times with its filtering residue, merge the filtrate of 12 extractions, it is thick that concentrating under reduced pressure is, place far-infrared oven to dry for 40-50 ℃, pulverize, cross 60 mesh sieves, get LCP, yield is 6.1%.

Embodiment 3, operate by Fig. 3 production technology: get the 50g Semen livistonae chinensis and really blend, place pressure cooker, add 2.4 liters in water, after boiling, cover pressurizing valve heating 10 minutes, (120 ℃ of kettle temperatures, pressure are 2kg) dries in the air to 70-80 ℃, filter, filtrate decompression concentrates, and 40-50 ℃ of oven dry are pulverized, cross 60 mesh sieves, the LCP yield is 5.9%.

Embodiment 4, press Fig. 4, and production technology is operated: get the 500g Semen livistonae chinensis and really add 12 liters in water, quick-freezing is to-20 ℃, after 24 hours, taking-up adds to 70-80 ℃ of speed rapidly and thaws, and boils 1 hour at 100 ℃, filters concentrating under reduced pressure, dry at 40-50 ℃, pulverize, cross 60 mesh sieves, the yield of LCP is 5.6%.Two, the zoopery of LCP:

1, anxious toxigenicity test: as shown in table 2, the mensuration table 2 of maximum tolerated dose (MTD)

Submitted sample	The censorship unit	Animal species	Experimental technique
				LCP extracting solution lot number 960688 crude drug amount 1.5g/ml	The inventor	Kunming mouse	20 of the mices of body weight 19 ± 1g, male and female half and half, irritate stomach on an empty stomach, amount 75g/kg has once irritated, observed 7 days continuously, animal peace and quiet, no abnormal do not have death, repeat once.This each amount is pressed body surface area conversion 346 times for human dosage.

2, the LCP extracting solution is to the inhibiting experimental result of in-vitro cell growth

(1) experimental technique: tetramethyl azo azoles salt (MTT) colorimetry

(2) experiment cell strain: Hela (people palace cancerous cell), Bca-61 (human breast cancer cell), NFC (normal adult fibrocyte).

(3) experimental result: as shown in table 3

Table 3 inhibitory rate of cell growth (%)

LCP concentration	????10％	????5％	????3％	??2％	????1％	??0.5％
							??Hela	????100	????100	????54.8	??55.3	????51.6	??36.5
??Bca—61	????100	????100	????33.7	??39.0	????25.4	??21.2
							??NFC	????100	????100	????10.8	??10.4	????0.0	??0.0

Experimental result shows: the LCP extracting solution (hereinafter to be referred as medicinal liquid) of 1-2% concentration all has inhibitory action to the growth of testing available Hela and Bca-61 cells, growth to normal adult fibrocyte (NFC) does not then have obvious influence, medicinal liquid is more obvious to the performance of Hela cell, and the medicinal liquid of 1% concentration can make suppression ratio reach 51.6%.

3, the zoopery of medicinal liquid vivo antitumor effect

(1) experimental technique:

1. the Kunming white mice is male, body weight 20-22g, S-180 mouse ascites cancerous cell 1 * 10 ⁵, and animal is divided into blank, contrast at random, 5 groups of low dosage, middle dosage, high doses etc., except that 5 of blank groups, other every group with 20 of animals, weighs before and after the experiment.

2. blank group is not inoculated ehrlich ascites carcinoma, only irritates stomach every day and gives the 0.2ml normal saline; Other is organized and irritates stomach 0.2ml medicinal liquid every day.

3. adopt common gastric infusion method, observe anticancer effect.

(2) experimental result: administration was carried out following detection to all mices after 7 days:

1. body weight: as shown in table 4:

Table 4 mice body weight change table

Group	Number of animals	Meansigma methods (g) before the experiment	Test meansigma methods (g) after 7 days	Average variability (%)
					Blank	????5	????19.98	????28.04	????+40.4
Contrast	????20	????19.08	????22.35	????+17.14
					Low dosage 0.1g	????17	????19.84	????21.30	????+7.36
Middle dosage 0.2g	????22	????21.30	????20.03	????-6.1
					High dose 0.3g	????20	????21.82	????19.40	????-10.93

2. laboratory observation result: as shown in table 5

Table 5

Group	Number of animals	Negative number of animals	Send out the cancer number of animals	Ascites cells average counter (1 * 10 6/ml)	Suppression ratio (%)
						Blank	????5	????0	????0		????—
Contrast	????20	????20	????20	????110.4	????0
						Low dosage 0.1g	????17	????8	????9	????60.4	????47.1
Middle dosage 0.2g	????22	????17	????5	????56.0	????77.2
						High dose 0.3g	????20	????16	????4	????76.0	????80.0

3. experiment conclusion: S-180 mouse ascites cancerous cell inoculation kunming mice is observed the medicinal liquid cancer suppressing action by traditional gastric infusion method, and any untoward reaction does not all appear in all animals in the experimentation, and proves by observing detection computations; In high agent cancer resistant effect the most obvious, and do not have evident difference between the dosage.

Three, medicinal liquid suppresses the immunology observation experiment of mouse ascites cancer S-180:

1, experimental principle: the T lymphocyte is subjected to specific antigen (as purified protein derivative (PPD) (PPD) or non-specific mitogen (as phytohaemagglutinin when In vitro culture, Semen Canavaliae A etc.) after the stimulation, cell volume can occur increases, metabolism is vigorous, albumen and nucleic acid are synthetic to be increased, and can divide red lymphoblast, be the lymphocyte transformation phenomenon, height according to lymhocyte transformation rate can react the cell immune function of human body level, therefore lymphocyte transformation test is widely used in one of index of measuring the body cell immunologic function, clinically as checking the damaged of cellular immune function, and the discussion of the various diseases cause of disease, the judgement of therapeutic effect etc., in the research of materia medica, be used to screen the Chinese medicine that influences cellular immune function.

2, experiment material:

Animal: Kunming mouse, supply by animal portion of the Capital University of Medical Sciences.

Medicine and reagent: cardiografin separating medium, 1640 nutritional solutions, calf serum, PHA, penicillin, streptomycin etc.

Other: the penicillin bottle, Switzerland's dyeing liquor, methanol, centrifuge, enumerator, microscope, calorstat etc.,

3, experimental technique

The lymphocyte transformation experimentation:

(1) morphological method: get mouse spleen and smash to pieces,, be placed in the culture fluid then with layering liquid isolated lymphocytes, add a certain amount of mitogenesis element, hatch for 37 ℃ and cultivated 72 hours, every day, jolting was twice, only remove supernatant, smear staining is observed lymphocytic morphological change.With head, body, tail three parts of high power lens or oily sem observation push jack,

(2) morphology and other standard, differentiate according to cell size, nuclear and endochylema feature etc.:

1. the cell volume increase is original 3-5 times;

2. the dyeing qualitative change is loose in the nuclear, 1-3 kernels can occur;

3. endochylema is abundant, and little cavity is arranged in the slurry, and the pseudopodium projection is arranged all around.Possess above 1-2 characteristic persons, the lymphocyte that can be defined as transforming, and therefrom calculate the percentage rate that is converted into blast cell Normal value that it is generally acknowledged conversion ratio is 60-70% (the clinical normal value of looking into human blood)

4, experimental technique:

(1) get Kunming mouse, body weight 20 ± 2.5g, male and female half and half are divided into 6 groups at random;

(2) 5 groups of experiment administration component, 12 every group Mus, 3 groups of controlled observation component, every group of 10 mices;

(3) every mouse peritoneal injection tumor 10 except that the blank group ^6-8, 0.2ml (every mice is only injected once), the same day gastric infusion, every 0.2ml once a day, (irritated stomach dosage difference for every group in continuous 4 days, low dosage 0.1ml, middle dosage 0.2ml, high dose 0.3ml,), after weighing in the 6th day, dissect extracting spleen cell, isolated lymphocytes is done In vitro culture, does the lymphocyte transformation experiment.

1. blank: do not inject oncocyte and any medicine, only do the contrast of healthy Mus.

2. negative control: only inject oncocyte 10 ^6-80.2ml, irritate stomach with normal saline,

3. positive control: only inject oncocyte 10 ^6-80.2ml, do not give any medicine and liquid, (4) mice cultivation situation is detailed to be shown in Table 6

Table 6 mice group medicine feed information slip

??LCP	Group	Number of animals	The inoculation oncocyte	Administration concentration and dosage	The administration natural law
						Experimental group	Dosage high dose in the low dosage	????12 ????12 ????12	Inoculation inoculation inoculation	1.1g/ml, 0.1ml/ 1.1g/ml, 0.2ml/ is only the same, and 0.3ml/ is only	????4 ????4 ????4
Matched group	Negative positive blank	????10 ????10 ????10	Inoculation is inoculated	Aseptic physiologic dose brine 0.2ml/ only	????4 ????4 ????4

Annotate: oncocyte is mouse ascites oncocyte S-1805, experimental data: as shown in table 7

The external lymhocyte transformation rate of table 7 (%)

LCP	Group	?1??2??3??4??5??6??7??8??9??10?11?12	??? X	???? X 2
					Experimental group	Dosage high dose in the low dosage	?60?49?55?61?42?47?42?57?55?56?59?53 ?60?56?49?54?53?56?59?55?48?46?53?66 ?78?85?74?76?85?76?72?83?77?84?79?86	?53.00 ?54.75 ?80.00	??2809.00 ??2997.56 ??6400.00
Matched group	Negative positive blank	?42?41?44?50?46?41?36?40?38?42 ?46?45?50?41?39?35?46?34?32?40 ?60?57?59?52?56?58?46?51?56?45	?42.20 ?40.80 ?54.00	??1780.84 ??1664.64 ??2916.00

The statistical disposition of experimental data: will test each group and negative control group and do sample average t check relatively on two with positive controls respectively, way is as follows: the t check relatively of (1) positive controls and negative control group:

P value＞0.05 though no significance meaning has promptly been injected ascites cells S-180 simultaneously, is irritated stomach and is not irritated stomach to the no significance influence of this experiment with normal saline.(2) experiment table 8 group statistical data and result: as shown in table 8

Table 8

The LCP group	Average conversion (%)	The t test value	The P value	Average weight value added (g)
					Dosage high dose in the low dosage	?55.00 ?54.75 ?80.00	????4.669 ????5.640 ????16.860	??＜0.01 ??＜0.01 ??＜0.01	????1.71 ????1.69 ????2.31

6, discuss: lymphocyte transformation experimental statistics data show, all can activate the vigor of mice body endolymph cell effectively, improve mice to ascites cells S-180 immunologic function, and wherein the high dose group effect is the most obvious.

Four, clinical model case is for example: the former powder of LCP gives protection against cancer, controls existing more than the 30 year history of cancer, has the people more than 120 that reaches of healing, survival according to incompletely statistics, and now wherein several model cases are listed below:

Case 1: in * * man, 54 years old, the teacher found about 3 months of the swollen thing of tongue body, clinical diagnosis is a squamous cell carcinoma, refusal is implemented excision, 2 week of medication the back lumps dwindle gradually, medication after 3 months the tongue body outward appearance recovered normally, he and recovers normal teaching.

Case 2: open * * man, 63 years old, cough, hemoptysis two wheat harvesting periods, CT is diagnosed as pulmonary carcinoma, certain provincial hospital hospitalization two months, and no obvious curative effects, occur indifferent, drowsiness, babble, breast CT shows metastatic lesion, with this medicine after 2 months, CT examination shows that the chest metastatic lesion disappears, and the chest films showed pulmonary lesions has been contracted to 1/3, and is existing still in the continuation treatment of taking medicine.

Case 3: the king * *: the man, 48 years old, cough, chest pain is shown as the pulmonary carcinoma centre type through CT examination, does not perform a surgical operation, and after 2 months, rabat shows the method focus and all disappears with this medicine, the uncomfortable reaction of existing discovery.

Case 4: beam * *: 32 years old, the woman because of finding the frequent pain of upper right abdomen, through CT examination, was diagnosed as hepatocarcinoma, belonged to massive type, and with this medicine 3 months, focus is dwindled gradually, and was existing still in the continuation medication treatment.

Case 5: woods * *: the man, 58 years old, found rectal cancer before 5 years, i.e. recurrence more than a year has focus to shift in CT scan shows, behind this medicine one wheat harvesting period after the underwent operative excision, CT scan shows the hepatic metastases foci disappearance, the patient is except that artificial anus's inconvenience, and it is no any unusual to live, and has survived more than 3 years.

Case 6: Zhao * *: the woman, 40 years old, after the discovery renal carcinoma, excised right kidney (pathology is not quite clear), find again after 1 year that focus extensively shifts, belong to patient with advanced cancer, pain is violent unusually, with this medicine after 2 months, patient's ordinary circumstance is good, and pain greatly alleviates, and is existing still in medication treatment.

Case 7: Hao * * woman, 68 years old, after finding breast carcinoma before 18 years, recurrence and extensively transfer again belong to end-stage patients after the underwent operative chemotherapy, with this medicine after half a year, metastatic lesion disappears, and has now survived 18 years, does not have any discomfort sensation on ordinary days and (annotates: meet the Summer Solstice every year, the two weeks of respectively taking medicine Winter Solstice can keep recurring never).

Claims (5)

1, the present invention is a kind of newfound anti-cancer, anticancer full-natural nutritive type health product and medicine, and this medicine and health care strain are extracted the former powder of Semen livistonae chinensis (being called for short LCP) that forms through the high-tech technology from babassu Semen livistonae chinensis fruit;

2, according to claim 1, be meant the band shell or can not extract the LCP of identical anticancer effect real from the Semen livistonae chinensis fruit with shell fruit;

3, according to claim 2, the Semen livistonae chinensis fruit after crushed, respectively with medicinal ethanol, quick-freezing speed thaw, the new technique of breaking cell wall such as ultrasound wave, multiple high temp boil, High Temperature High Pressure is disposable;

4, according to claim 3, medicinal alcohol concentration is 98-50%; Multiple high temp boils and means 100 ℃; Its temperature of High Temperature High Pressure is that 120 ℃, pressure are 2kg;

5, according to claim 1, as Chinese medicine or its zooperal cancer suppressing ratio of health product greater than 80% clinical effective rate more than 85%.

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