CN100388920C - Oral administration dual polysaccharide Chinese medicine and its preparation method - Google Patents
Oral administration dual polysaccharide Chinese medicine and its preparation method Download PDFInfo
- Publication number
- CN100388920C CN100388920C CNB2004100395924A CN200410039592A CN100388920C CN 100388920 C CN100388920 C CN 100388920C CN B2004100395924 A CNB2004100395924 A CN B2004100395924A CN 200410039592 A CN200410039592 A CN 200410039592A CN 100388920 C CN100388920 C CN 100388920C
- Authority
- CN
- China
- Prior art keywords
- chinese medicine
- polysaccharide
- chinese
- preparation
- ethanol
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Abstract
The present invention relates to a Chinese medicine oral administration preparation of dual polysaccharide and a preparation method thereof. The present invention is characterized in that the Chinese medicine oral administration preparation comprises two kinds of Chinese medicine A and Chinese medicine B. The Chinese medicine A is astragalus polysaccharide, and the content of the Chinese medicine A is 50 to 90%; the Chinese medicine B is radices ophiopogonis polysaccharide, and the content of the Chinese medicine B is 50 to 90%. The Chinese medicine A and the Chinese medicine B are active parts of plant drugs. The present invention has the preparation method that a medicinal material A is weighed to be boiled in water, filtered and concentrated, ethanol is added in the medicinal material A to carry out standing filtration, sediment is taken to be dried at the low temperature, the sediment is dissolved in distilled water to be boiled and filtrated, the ethanol is added in the filter liquor to carry out standing filtration, the sediment is washed by 80% of ethanol to be dried at the low temperature, and a crude product is obtained; the crude product polysaccharide is put in the distilled water to be stirred, tannic acid is added in the distilled water to be boiled and centrifugated to remove the sediment, the tannic acid is added until the physic liquor has no turbidity, and then, the tannic acid is removed to prepare an active component of the medicinal material A; a medicinal material B is taken, drying stem blocks are chopped to be decocted twice by slightly boiling water, the decocted liquid is concentrated, the liquid is extracted for three times by 5 times of 80% of ethanol reflux, the ethanol is recovered, residue is heated and extracted by 5 times of distilled water, the filter liquor is stirred uniformly by 0.1% of active carbon to carry out standing filtration, the filter liquor passes through an ultrafiltration membrane of which the molecular weight is.
Description
Technical field
The present invention relates to a kind of 2 polysaccharide for reducing blood sugar oral preparation of Chinese traditional medicinal and preparation method thereof, belong to medical technology.
Background technology
China has more than diabetics 4,000 ten thousand people at present, and this numeral has increased by 5 times in nearest 10 years.This disease death that causes because of blood sugar disorders has become one of Chinese's main causes of death, is only second to cardiovascular and cerebrovascular disease and cancer.The traditional Chinese medical science thinks that the cause of disease of diabetes mainly is: eating and drinking without temperance; Disorder of emotion; Deficiency of kidney-YIN.Diabetes are a kind of metabolism disturbance syndromes due to the multiple reason, causing blood glucose, glucose in urine to raise with the relative or absolute deficiency of insulin is characteristics, the diabete of traditional Chinese medical science meaning, the clinical symptoms such as polydipsia, polyphagia, polyuria, the fatigue of becoming thin that occur, especially middle age is with fatigue and weakness, nocturia is a main symptom, if long-term Western medicine control, just antibody can appear, often can cause the heart, cerebrovascular, kidney, optical fundus, retina and nervous system lesion, complication such as ketoacidosis hyperosmolar coma can take place when serious.The traditional Chinese medical science thinks that the cause of disease of diabetes mainly is: eating and drinking without temperance; Disorder of emotion; Deficiency of kidney-YIN.
Purpose of the present invention is according to motherland's Chinese medicine theory, provides a kind of blood sugar lowering oral preparation of Chinese traditional medicinal and preparation method thereof that is used in conjunction with the pharmacology of diabetes.This oral preparation of Chinese traditional medicinal is that pharmaceutically active ingredient share in two kinds, and the type of YIN-deficiency and interior-heat diabetes are had the effect of clearing away heat and nourishing YIN, can obviously alleviate polydipsia polyphagia polyuria symptom, makes the patient recover ideal body weight; This medicine also has the QI invigorating effect, can effectively improve weak symptom, the while activating blood circulation to dissipate blood stasis and dredge the collateral, and diabetic nephropathy, diabetic renal papillary necrosis, diabetic peripheral neuropathy that diabetic microvascular complication is caused all have good efficacy; We can also increase Abwehrkraft des Koepers in addition, improve immunity, have the merit of strengthening vital QI to eliminate pathogenic factors.Thereby diabetes complicated various infection all there is good effect.
Summary of the invention
1, the invention provides the oral preparation of Chinese traditional medicinal of 2 polysaccharide for reducing blood sugar, it is characterized in that containing two kinds of Chinese medicines: Chinese medicine A and Chinese medicine B.Chinese medicine A is the active component astragalus polysaccharides of the Chinese crude drug Radix Astragali, and content is 50%～90%, and Chinese medicine B is the active component Radix Ophiopogonis polysaccharide of Chinese crude drug Radix Ophiopogonis, and content is 50%～90%.The set of dispense of Chinese medicine A and Chinese medicine B than (weight portion) in crude drug: A: B=10: 90～90: 10.
2, the present invention also provides the preparation method of the oral preparation of Chinese traditional medicinal of 2 polysaccharide for reducing blood sugar, it is characterized in that: the preparation method of Chinese medicine A and Chinese medicine B is as follows:
The preparation method of Chinese medicine A: get Milkvetch Root, decoct with water three times, each 2 hours, collecting decoction filtered, and is concentrated into relative density 1.15～1.20 (50 ℃), and adding ethanol, to make pure content be 80%, left standstill 24 hours, filters, and gets precipitation.The precipitate cold drying is dissolved in the dry thing of gained in the distilled water, heated and boiled 10 minutes, and filtered while hot, filtrate adds ethanol, and to make pure content be 80%, left standstill 24 hours, filters, and precipitate is with 80% washing with alcohol three times, and cold drying gets crude product.The crude product polysaccharide is put in the distilled water, added tannic acid (1%) under stirring, boil the centrifugal precipitate that discards in back, add tannic acid to medicinal liquid again and do not produce till the muddiness.Continued to boil 15 minutes, and added active carbon, stirred 15 minutes, filter, filtrate adds ethanol, and to make pure content be about 70%, left standstill 24 hours, filter, precipitation, precipitate do not contain tannic acid to the liquid with 70% washing with alcohol till.
The preparation method of Chinese medicine B: get the Radix Ophiopogonis medical material, dry stem piece chopping, decoct twice with little boiling water, with the liquid concentration after decocting, with 5 times 80% alcohol reflux 3 times, reclaim ethanol, residue is with the distilled water heating extraction of 5 times of amounts, and filtrate stirs evenly with 0.1% active carbon, leave standstill filtration, filtrate is 30000 ultrafilter membrane by molecular weight, is evaporated to the 1KG/ liter, adds 4 times of amount dehydrated alcohol, leave standstill filtration, get supernatant, use dehydrated alcohol, washing with acetone 4 times, 60 degrees centigrade of vacuum dryings.
3, the present invention's 2 polysaccharide for reducing blood sugar oral preparation of Chinese traditional medicinal, confirm through clinical trial, this oral preparation of Chinese traditional medicinal can not only effectively improve subjective symptomss such as patient's polydipsia, polyphagia, polyuria, the soreness of waist, five hearts are scorching, limb cold, can also effectively control on an empty stomach and post-prandial glycemia, improves every biochemical indicator.
4, oral preparation of Chinese traditional medicinal of the present invention is that pharmaceutically active ingredient share in two kinds, and the type of YIN-deficiency and interior-heat diabetes are had the effect of clearing away heat and nourishing YIN, can obviously alleviate polydipsia polyphagia polyuria symptom, makes the patient recover ideal body weight; This medicine also has the QI invigorating effect, can effectively improve weak symptom, the while activating blood circulation to dissipate blood stasis and dredge the collateral, and diabetic nephropathy, diabetic renal papillary necrosis, diabetic peripheral neuropathy that diabetic microvascular complication is caused all have good efficacy; We can also increase Abwehrkraft des Koepers in addition, improve immunity, have the merit of strengthening vital QI to eliminate pathogenic factors; Thereby diabetes complicated various infection all there is good effect.
Drug mechanism
Oral preparation of Chinese traditional medicinal of the present invention can reduce blood glucose in diabetic rats level due to the intraperitoneal injection streptozotocin, improves its carbohydrate tolerance, reduces albumen saccharifying value and improve the diabetes rat insulin content, and these effects obviously are better than glyburide; Have the LPO content, the SOD activity improving that reduce experimental diabetic rats, improve the activity of GSH-Px in the pancreas simultaneously, thereby suppress the intravital oxygen free radical reaction of diabetes rat; Effect with disturbance state of the gonad function that improves male and female diabetes rat.
Oral preparation of Chinese traditional medicinal of the present invention has the effect of clearing away heat and nourishing YIN to the type of YIN-deficiency and interior-heat diabetes, can obviously alleviate polydipsia polyphagia polyuria symptom, makes the patient recover ideal body weight.Also have the QI invigorating effect, can effectively improve weak symptom, the while activating blood circulation to dissipate blood stasis and dredge the collateral, diabetic nephropathy, diabetic renal papillary necrosis, diabetic peripheral neuropathy that diabetic microvascular complication is caused all have good efficacy.Can increase Abwehrkraft des Koepers, improve immunity, have the merit of strengthening vital QI to eliminate pathogenic factors.Thereby diabetes complicated various infection all there is good effect.
The pharmacological research data
1. pharmacodynamics data:
Treat the effectiveness of diabetes and inquire into its mechanism of action for confirmation oral preparation of Chinese traditional medicinal of the present invention, we use the streptozotocin intraperitoneal injection and duplicate the rat diabetes model, and blood and some tissue of rat carried out a series of experiments.These experiments include: the saccharifying value of carbohydrate tolerance, serum insulin content, blood plasma fructosamine content, hemoglobin and histone, lipid peroxide (LPO) content, superoxide dismutase (SOD) activity, glutathion oxide enzyme (GSH-PX) activity, plasma testosterone and estradiol content.The result shows that oral preparation of Chinese traditional medicinal of the present invention can reduce blood glucose in diabetic rats level due to the intraperitoneal injection streptozotocin, improve its carbohydrate tolerance, reduces albumen saccharifying value and improve the diabetes rat insulin content, and these effects obviously are better than glyburide; Have the LPO content, the SOD activity improving that reduce experimental diabetic rats, improve the activity of GSH-Px in the pancreas simultaneously, thereby suppress the intravital oxygen free radical reaction of diabetes rat; Effect with disturbance state of the gonad function that improves male and female diabetes rat.
Table 1. streptozotocin lumbar injection respectively organize after 3 days rat blood sugar content and carbohydrate tolerance (X ± SD, n=10)
Annotate:
aCompare P＜0.0001 with the normal control group
Table 2. respectively organize rat plasma insulin content, blood plasma fructosamine and Hb saccharifying value and pancreas and kidney protein saccharifying value testing result (X ± SD, n=10)
Annotate:
aCompare P＜0.0001 with model control group; Compare with the normal control group,
bP＜0.0001,
cP＜0.01; Compare with the glyburide group,
dP＜0.01
Each organizes rat plasma LPO content, SOD and the active measurement result of GSH-Px
Table 3 respectively organize rat plasma LPO content, SOD and the active measurement result of GSH-Px (X ± SD, n=10)
Annotate:
aCompare P＜0.0001 with model control group; Compare with the normal control group,
bP＜0.0001,
cP＜0.01; Compare with the glyburide group,
dP＜0.01
2. safety testing data
Emergency toxicology research
Body weight 18-22g Kunming kind white mice each 20, male and female half and half, give maximum volume and the Cmax capsule is irritated the harmonization of the stomach intraperitoneal injection, observed continuously 7 days.Capsule filling stomach of the present invention (total dosage is 66.66g/Kg) mice any unusual performance do not occur and there is no an example death.So capsule gastric infusion LD50＞66.66g/Kg of the present invention.Intraperitoneal injection drug (total dosage is 12.5g/Kg) was observed 7 days continuously, and writhing response appears in mice 5-10min after administration, and none example is dead.
Long-term toxicologic study
In order to study the long term toxicity effect of oral preparation of Chinese traditional medicinal of the present invention to animal, we use the capsule of the present invention of three kinds of various dose, and (dosage is respectively: 20.0/kg, 10.0g/kg, 5.0g/kg) give Wistar rat oral gavage 3 months continuously, 6 times weekly, the normal control group gives the normal saline of same volume and irritates stomach, and the experimental session rat does not have death.Experimental result confirms, the general state of each dosage group rat and rats in normal control group, all Non Apparent Abnormality performances such as outward appearance sign, behavioral activity, feces character etc.An index such as each dosage group rat serum routine urinalysis, blood biochemical and meeting renal function is no abnormal, and compares statistics with rats in normal control group and do not have significant difference.The main organs of heavy dose of group (20.0g/kg) rat after one's own heart, internal organs such as liver, spleen, lung, adrenal gland, testis, uterus observe Non Apparent Abnormality and each organ index of each dosage group rat and normal control group no difference of science of statistics down through naked eyes and light microscopic.The dosage (this dosage is 100 times of clinical equivalent dosage) that experiment discloses 20.0g/kg.d is the safe dose scope.
3. clinical research data
The tcm syndrome integration relatively before two groups of treatments
Check through t: each organizes tcm symptom integrated value P＞0.05, and comparability is arranged.
Two groups of clinical symptom treatment backs relatively
Result: X
2 I-III=7.65 P＜0.01 X
2 II-III=4.33 P＜0.05
From table as can be known: the tcm clinical practice symptom of Chinese medicine I group through treatment after, treatment I group total effective rate reaches 90.5%, with Western medicine II group relatively, significance P＜0.01～0.05 is arranged.
Two groups of laboratory indexes change
Annotate: compare between * P＜0.05 * * P＜0.01 * * * P＜0.001 Δ group: P＜0.05, down together
Above index shows, two groups of medicines self relatively, to blood glucose, 2h blood glucose all makes its decline effect (P＜0.05～0.01) after the meal.
Relatively, I group, II organize relatively between group, are reducing fasting glucose and are having two groups of blood biochemistry index of significant difference to change on the 2h blood glucose after the meal
From table as can be known, after taking capsule of the present invention, the patient increases on blood insulin content, and relatively there were significant differences for self, decreases on the contrary before treating and take the insulin level of glyburide group.
Each content of organizing patient's fructosamine and glycolated hemoglobin all has decline after experiment, there were significant differences, and the content of the glycolated hemoglobin of capsule group wherein of the present invention approaches normally.
Each organize the Aeg treat before and after plasma estradiol and testosterone concentration change (X ± S)
Annotate: self relatively
*P＜0.05,
*P＜0.01
Each organize the aeg treat before and after plasma estradiol and testosterone concentration change (X ± S)
Respectively organize patient's male estradiol before experiment as seen from table and all be higher than normal group, testosterone all is lower than normal group, simultaneously ratio (the E of estradiol and testosterone
2/ T) also be higher than normal group; Estradiol content obviously reduces after taking capsule of the present invention, and testosterone concentration obviously raises, thereby E
2/ T also significantly reduces; Yet the patient of glyburide group, these three indexs have no significant change before and after experiment.
Four, clinical research summary
Confirm that through clinical trial oral preparation of Chinese traditional medicinal of the present invention can not only effectively improve subjective symptomss such as patient's polydipsia, polyphagia, polyuria, Yao Acid, dysphoria with feverish sensation in the chest palms and soles, limb cold, can also effectively control on an empty stomach and post-prandial glycemia, improve every biochemical indicator.
Capsule traditional Chinese medical science disease total effective rate of the present invention is 90.5%, and wherein obvious effective rate is 42.3%.The matched group obvious effective rate is that 33.3% total effective rate is 66.6%.Treatment I group compares with matched group, significant difference is arranged and single from the disease angle analysis, and the treatment group is to syndrome of deficiency of both qi and yin such as weak, dysphoria with feverish sensation in the chest palms and soles better effects if.
Treatment diabetes and chronic complicating diseases clinical efficacy are reliable.The plasma insulin content of taking capsule patient of the present invention (I group) has remarkable rising.Patient's saccharification hemoglobin content approaches normal level.Take to compare on the contrary obviously before patient's insulin level and the experiment of glyburide (II group) and reduce, patient's saccharification hemoglobin content is higher than normal level.
Male blood plasma estradiol (E2) and testosterone (T) Determination on content result show, take capsule of the present invention after estradiol content obviously reduce, testosterone concentration obviously raises; Yet this index of the patient of glyburide group has no significant change before and after experiment.Each is organized women's plasma estradiol (E2) and testosterone (T) Determination on content result and shows, testosterone content obviously reduced after each was organized female patients and takes capsule of the present invention, and the estradiol amount obviously raises; Yet this index of the patient of glyburide group has no significant change before and after experiment.
Through the years of researches evidences, no matter still be clinical efficacy from zoopery on, its effect is all good, has no side effect.Medicine is fewer but better, and market prospect is wide, and medicament sources is convenient.
A kind of hypoglycemic oral preparation of Chinese traditional medicinal of the present invention can be a solution, suspensoid, tablet, capsule, granule, pill, powder, drop pill; Preferred capsule, tablet, granule; More preferably capsule.Composition A and B can be mixed in suitable carriers, in diluent or the excipient.Specific embodiment is as follows, includes but not limited to the following example.
Embodiment 1
2 polysaccharide for reducing blood sugar Chinese medicinal capsule agent is characterized in that containing two kinds of Chinese medicine A and B, its set of dispense than (weight portion) in crude drug: A: B=60: 40
The preparation method of the Chinese medicinal capsule agent of the present invention's 2 polysaccharide for reducing blood sugar is characterized in that: the preparation method of Chinese medicine A and Chinese medicine B is as follows:
The preparation method of Chinese medicine A: get Milkvetch Root, decoct with water three times, each 2 hours, collecting decoction filtered, and is concentrated into relative density 1.15 ~ 1.20 (50 ℃), and adding ethanol, to make pure content be 80%, left standstill 24 hours, filters, and gets precipitation.The precipitate cold drying is dissolved in the dry thing of gained in the distilled water, heated and boiled 10 minutes, and filtered while hot, filtrate adds ethanol, and to make pure content be 80%, left standstill 24 hours, filters, and precipitate is with 80% washing with alcohol three times, and cold drying gets crude product.The crude product polysaccharide is put in the distilled water, added tannic acid (1%) under stirring, boil the centrifugal precipitate that discards in back, add tannic acid to medicinal liquid again and do not produce till the muddiness.Continued to boil 15 minutes, and added active carbon, stirred 15 minutes, filter, filtrate adds ethanol, and to make pure content be about 70%, left standstill 24 hours, filter, precipitation, precipitate do not contain tannic acid to the liquid with 70% washing with alcohol till.
The preparation method of Chinese medicine B: get the Radix Ophiopogonis medical material, dry stem piece chopping, decoct twice with little boiling water, with the liquid concentration after decocting, with 5 times 80% alcohol reflux 3 times, reclaim ethanol, residue is with the distilled water heating extraction of 5 times of amounts, and filtrate stirs evenly with 0.1% active carbon, leave standstill filtration, filtrate is 30000 ultrafilter membrane by molecular weight, is evaporated to the 1KG/ liter, adds 4 times of amount dehydrated alcohol, leave standstill filtration, get supernatant, use dehydrated alcohol, washing with acetone 4 times, 60 degrees centigrade of vacuum dryings.
Incapsulate by the formula proportion mix homogeneously, reinstall in the bottle and seal.
Embodiment 2
2 polysaccharide for reducing blood sugar Chinese medicine granules is characterized in that containing two kinds of Chinese medicine A and B, its set of dispense than (weight portion) in crude drug: A: B=60: 40
The preparation method of the Chinese medicinal capsule agent of the present invention's 2 polysaccharide for reducing blood sugar is characterized in that: the preparation method of Chinese medicine A and Chinese medicine B is as follows:
The preparation method of Chinese medicine A: get Milkvetch Root, decoct with water three times, each 2 hours, collecting decoction filtered, and is concentrated into relative density 1.15 ~ 1.20 (50 ℃), and adding ethanol, to make pure content be 80%, left standstill 24 hours, filters, and gets precipitation.The precipitate cold drying is dissolved in the dry thing of gained in the distilled water, heated and boiled 10 minutes, and filtered while hot, filtrate adds ethanol, and to make pure content be 80%, left standstill 24 hours, filters, and precipitate is with 80% washing with alcohol three times, and cold drying gets crude product.The crude product polysaccharide is put in the distilled water, added tannic acid (1%) under stirring, boil the centrifugal precipitate that discards in back, add tannic acid to medicinal liquid again and do not produce till the muddiness.Continued to boil 15 minutes, and added active carbon, stirred 15 minutes, filter, filtrate adds ethanol, and to make pure content be about 70%, left standstill 24 hours, filter, precipitation, precipitate do not contain tannic acid to the liquid with 70% washing with alcohol till.
The preparation method of Chinese medicine B: get the Radix Ophiopogonis medical material, dry stem piece chopping, decoct twice with little boiling water, with the liquid concentration after decocting, with 5 times 80% alcohol reflux 3 times, reclaim ethanol, residue is with the distilled water heating extraction of 5 times of amounts, and filtrate stirs evenly with 0.1% active carbon, leave standstill filtration, filtrate is 30000 ultrafilter membrane by molecular weight, is evaporated to the 1KG/ liter, adds 4 times of amount dehydrated alcohol, leave standstill filtration, get supernatant, use dehydrated alcohol, washing with acetone 4 times, 60 degrees centigrade of vacuum dryings.
Press the formula proportion mix homogeneously, add adjuvant, make soft material in right amount with ethanol, the system granule, cold drying behind the granulate, is distributed into bag.
Embodiment 3
2 polysaccharide for reducing blood sugar Chinese medicinal tablets is characterized in that containing two kinds of Chinese medicine A and B, its set of dispense than (weight portion) in crude drug: A: B=60: 40
The preparation method of the Chinese medicinal capsule agent of the present invention's 2 polysaccharide for reducing blood sugar is characterized in that: the preparation method of Chinese medicine A and Chinese medicine B is as follows:
The preparation method of Chinese medicine A: get Milkvetch Root, decoct with water three times, each 2 hours, collecting decoction filtered, and is concentrated into relative density 1.15 ~ 1.20 (50 ℃), and adding ethanol, to make pure content be 80%, left standstill 24 hours, filters, and gets precipitation.The precipitate cold drying is dissolved in the dry thing of gained in the distilled water, heated and boiled 10 minutes, and filtered while hot, filtrate adds ethanol, and to make pure content be 80%, left standstill 24 hours, filters, and precipitate is with 80% washing with alcohol three times, and cold drying gets crude product.The crude product polysaccharide is put in the distilled water, added tannic acid (1%) under stirring, boil the centrifugal precipitate that discards in back, add tannic acid to medicinal liquid again and do not produce till the muddiness.Continued to boil 15 minutes, and added active carbon, stirred 15 minutes, filter, filtrate adds ethanol, and to make pure content be about 70%, left standstill 24 hours, filter, precipitation, precipitate do not contain tannic acid to the liquid with 70% washing with alcohol till.
The preparation method of Chinese medicine B: get the Radix Ophiopogonis medical material, dry stem piece chopping, decoct twice with little boiling water, with the liquid concentration after decocting, with 5 times 80% alcohol reflux 3 times, reclaim ethanol, residue is with the distilled water heating extraction of 5 times of amounts, and filtrate stirs evenly with 0.1% active carbon, leave standstill filtration, filtrate is 30000 ultrafilter membrane by molecular weight, is evaporated to the 1KG/ liter, adds 4 times of amount dehydrated alcohol, leave standstill filtration, get supernatant, use dehydrated alcohol, washing with acetone 4 times, 60 degrees centigrade of vacuum dryings.
Press the formula proportion mix homogeneously, add a small amount of magnesium stearate and other adjuvants, compacting is distributed into bag in flakes.
Embodiment 4
2 polysaccharide for reducing blood sugar Chinese medicinal soft capsule agent is characterized in that containing two kinds of Chinese medicine A and B, its set of dispense than (weight portion) in crude drug: A: B=60: 40
The preparation method of the Chinese medicinal capsule agent of the present invention's 2 polysaccharide for reducing blood sugar is characterized in that: the preparation method of Chinese medicine A and Chinese medicine B is as follows:
The preparation method of Chinese medicine A: get Milkvetch Root, decoct with water three times, each 2 hours, collecting decoction filtered, and is concentrated into relative density 1.15 ~ 1.20 (50 ℃), and adding ethanol, to make pure content be 80%, left standstill 24 hours, filters, and gets precipitation.The precipitate cold drying is dissolved in the dry thing of gained in the distilled water, heated and boiled 10 minutes, and filtered while hot, filtrate adds ethanol, and to make pure content be 80%, left standstill 24 hours, filters, and precipitate is with 80% washing with alcohol three times, and cold drying gets crude product.The crude product polysaccharide is put in the distilled water, added tannic acid (1%) under stirring, boil the centrifugal precipitate that discards in back, add tannic acid to medicinal liquid again and do not produce till the muddiness.Continued to boil 15 minutes, and added active carbon, stirred 15 minutes, filter, filtrate adds ethanol, and to make pure content be about 70%, left standstill 24 hours, filter, precipitation, precipitate do not contain tannic acid to the liquid with 70% washing with alcohol till.
The preparation method of Chinese medicine B: get the Radix Ophiopogonis medical material, dry stem piece chopping, decoct twice with little boiling water, with the liquid concentration after decocting, with 5 times 80% alcohol reflux 3 times, reclaim ethanol, residue is with the distilled water heating extraction of 5 times of amounts, and filtrate stirs evenly with 0.1% active carbon, leave standstill filtration, filtrate is 30000 ultrafilter membrane by molecular weight, is evaporated to the 1KG/ liter, adds 4 times of amount dehydrated alcohol, leave standstill filtration, get supernatant, use dehydrated alcohol, washing with acetone 4 times, 60 degrees centigrade of vacuum dryings.
Press the formula proportion mix homogeneously, add adjuvant, make soft capsule, packing.
Embodiment 5
2 polysaccharide for reducing blood sugar Traditional Chinese Medicine Dropping Pill is characterized in that containing two kinds of Chinese medicine A and B, its set of dispense than (weight portion) in crude drug: A: B=60: 40
The preparation method of the Chinese medicinal capsule agent of the present invention's 2 polysaccharide for reducing blood sugar is characterized in that: the preparation method of Chinese medicine A and Chinese medicine B is as follows:
The preparation method of Chinese medicine A: get Milkvetch Root, decoct with water three times, each 2 hours, collecting decoction filtered, and is concentrated into relative density 1.15 ~ 1.20 (50 ℃), and adding ethanol, to make pure content be 80%, left standstill 24 hours, filters, and gets precipitation.The precipitate cold drying is dissolved in the dry thing of gained in the distilled water, heated and boiled 10 minutes, and filtered while hot, filtrate adds ethanol, and to make pure content be 80%, left standstill 24 hours, filters, and precipitate is with 80% washing with alcohol three times, and cold drying gets crude product.The crude product polysaccharide is put in the distilled water, added tannic acid (1%) under stirring, boil the centrifugal precipitate that discards in back, add tannic acid to medicinal liquid again and do not produce till the muddiness.Continued to boil 15 minutes, and added active carbon, stirred 15 minutes, filter, filtrate adds ethanol, and to make pure content be about 70%, left standstill 24 hours, filter, precipitation, precipitate do not contain tannic acid to the liquid with 70% washing with alcohol till.
The preparation method of Chinese medicine B: get the Radix Ophiopogonis medical material, dry stem piece chopping, decoct twice with little boiling water, with the liquid concentration after decocting, with 5 times 80% alcohol reflux 3 times, reclaim ethanol, residue is with the distilled water heating extraction of 5 times of amounts, and filtrate stirs evenly with 0.1% active carbon, leave standstill filtration, filtrate is 30000 ultrafilter membrane by molecular weight, is evaporated to the 1KG/ liter, adds 4 times of amount dehydrated alcohol, leave standstill filtration, get supernatant, use dehydrated alcohol, washing with acetone 4 times, 60 degrees centigrade of vacuum dryings.
Press the formula proportion mix homogeneously, add adjuvant, make drop pill, packing.
Claims (9)
1.2 the oral preparation of Chinese traditional medicinal of polysaccharide for reducing blood sugar, it is characterized in that containing two kinds of Chinese medicines: Chinese medicine A and Chinese medicine B, Chinese medicine A is the active component astragalus polysaccharides of the Chinese crude drug Radix Astragali, content is 50%～90%, Chinese medicine B is the active component Radix Ophiopogonis polysaccharide of Chinese crude drug Radix Ophiopogonis, content is 50%～90%, and the set of dispense of Chinese medicine A and Chinese medicine B is than counting A with the weight portion of crude drug: B=10: 90～90: 10.
2. the oral preparation of Chinese traditional medicinal of 2 polysaccharide for reducing blood sugar of claim 1, wherein, the set of dispense of Chinese medicine A and Chinese medicine B is than counting A with the weight portion of crude drug: B=20: 80～80: 20.
3. the oral preparation of Chinese traditional medicinal of 2 polysaccharide for reducing blood sugar of claim 1, wherein, the set of dispense of Chinese medicine A and Chinese medicine B is than counting A with the weight portion of crude drug: B=30: 70～70: 30.
4. the oral preparation of Chinese traditional medicinal of 2 polysaccharide for reducing blood sugar of claim 1, wherein, the set of dispense of Chinese medicine A and Chinese medicine B is than counting A with the weight portion of crude drug: B=40: 60～60: 40.
5. the oral preparation of Chinese traditional medicinal of 2 polysaccharide for reducing blood sugar of claim 1, wherein, the set of dispense of Chinese medicine A and Chinese medicine B is than counting A with the weight portion of crude drug: B=50: 50～50: 50.
6. the oral preparation of Chinese traditional medicinal of 2 polysaccharide for reducing blood sugar of claim 1, it is characterized in that: the preparation method of Chinese medicine A and Chinese medicine B is as follows:
The preparation method of Chinese medicine A: get Milkvetch Root, decoct with water three times, each 2 hours, collecting decoction filters, and is concentrated into relative density 1.15～1.20 under 50 ℃, adding ethanol, to make pure content be 80%, left standstill 24 hours, filters, get precipitation, the precipitate cold drying is dissolved in the dry thing of gained in the distilled water, heated and boiled 10 minutes, filtered while hot, filtrate add ethanol to make pure content are 80%, leave standstill 24 hours, filter, precipitate is with 80% washing with alcohol three times, and cold drying gets crude product, and the crude product polysaccharide is put in the distilled water, add 1% tannic acid under stirring, boil the centrifugal precipitate that discards in back, add tannic acid to medicinal liquid again and do not produce till the muddiness, continued to boil 15 minutes, add active carbon, stirred 15 minutes, and filtered, filtrate adds ethanol, and to make pure content be about 70%, left standstill 24 hours, filter, precipitation, precipitation do not contain to the liquid till the tannic acid with 70% washing with alcohol;
The preparation method of Chinese medicine B: get the Radix Ophiopogonis medical material, dry stem piece chopping, decoct twice with little boiling water, with the liquid concentration after decocting, with 5 times 80% alcohol reflux 3 times, reclaim ethanol, residue is with the distilled water heating extraction of 5 times of amounts, and filtrate stirs evenly with 0.1% active carbon, leave standstill filtration, filtrate is 30000 ultrafilter membrane by molecular weight, is evaporated to the 1KG/ liter, adds 4 times of amount dehydrated alcohol, leave standstill filtration, get supernatant, use dehydrated alcohol, washing with acetone 4 times, 60 degrees centigrade of vacuum dryings.
7. the oral preparation of Chinese traditional medicinal of 2 polysaccharide for reducing blood sugar of claim 1 is solution, suspensoid, tablet, capsule, granule, pill, powder, drop pill.
8. the oral preparation of Chinese traditional medicinal of 2 polysaccharide for reducing blood sugar of claim 7 is capsule, tablet, and granule:
9. the oral preparation of Chinese traditional medicinal of 2 polysaccharide for reducing blood sugar of claim 8 is capsule.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2004100395924A CN100388920C (en) | 2004-02-19 | 2004-02-19 | Oral administration dual polysaccharide Chinese medicine and its preparation method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2004100395924A CN100388920C (en) | 2004-02-19 | 2004-02-19 | Oral administration dual polysaccharide Chinese medicine and its preparation method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1657054A CN1657054A (en) | 2005-08-24 |
| CN100388920C true CN100388920C (en) | 2008-05-21 |
Family
ID=35006884
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB2004100395924A Expired - Fee Related CN100388920C (en) | 2004-02-19 | 2004-02-19 | Oral administration dual polysaccharide Chinese medicine and its preparation method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN100388920C (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103784727B (en) * | 2014-02-25 | 2017-03-22 | 湖南希尔天然药业有限公司 | Folium mori compound preparation and application thereof |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4795742A (en) * | 1985-09-24 | 1989-01-03 | Yaguang Liu | Therapeutic composition from plant extracts |
-
2004
- 2004-02-19 CN CNB2004100395924A patent/CN100388920C/en not_active Expired - Fee Related
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4795742A (en) * | 1985-09-24 | 1989-01-03 | Yaguang Liu | Therapeutic composition from plant extracts |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1657054A (en) | 2005-08-24 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103566350B (en) | A kind of pharmaceutical composition with hypoglycemic effect | |
| CN102271698A (en) | Pharmaceutical composition and use for preparing medicament thereof | |
| CN100348220C (en) | Chinese materia medica preparation for treating diabetic nephropathy, and preparation method | |
| CN104116990A (en) | Medicinal composition for treating diabetes mellitus and preparation method thereof | |
| CN102631595B (en) | Medicament for treating II-type diabetes mellitus and preparation method thereof | |
| CN100482237C (en) | Polysaccharid glucoside Chinese medicinal oral preparation and its making method thereof | |
| CN101167863B (en) | Proprietary Chinese medicine for treating diabetes and nephrosis and preparing method thereof | |
| CN106692742A (en) | Traditional Chinese compound medicine for regulating blood sugar and preparation method of traditional Chinese compound medicine | |
| CN101336965B (en) | Chinese prepared medicine for improving sugar tolerance and reducing blood sugar and preparation method thereof | |
| CN101347543A (en) | Chinese medicine compound and preparation method thereof | |
| CN100388920C (en) | Oral administration dual polysaccharide Chinese medicine and its preparation method | |
| CN1265805C (en) | Chinese medicine oral preparation for lonering blood sugar and its preparation method | |
| CN113476561A (en) | Traditional Chinese medicine formula for treating diabetes | |
| CN100528186C (en) | Process for preparing Chinese medicine compound injection for treating chronic renal failure and use | |
| CN101120977A (en) | Medicine for treating tumor | |
| CN1253190C (en) | Medicine for treating diabetes | |
| CN108404088B (en) | Traditional Chinese medicine for treating type II diabetes and preparation method thereof | |
| CN103536879B (en) | A kind of pharmaceutical composition and preparation method for the treatment of diabetes | |
| CN102048132B (en) | Health-care food for reducing blood sugar | |
| CN1742879A (en) | Chinese medicine for reducing blood sugar and preparation thereof | |
| CN105326929A (en) | Rhizoma coptidis and panax notoginseng traditional Chinese medicinal oral preparation and preparation method thereof | |
| CN1657055A (en) | Traditional Chinese oral preparation of ophiopogon and coptisine and its preparation method | |
| CN103550290B (en) | Chinese medicinal composition for treating type 2 diabetes | |
| CN115779046B (en) | Traditional Chinese medicine composition, traditional Chinese medicine preparation and application for preventing and treating diabetes | |
| CN115531482B (en) | Application of ginseng body-building oral liquid in preparation of medicine for preventing and treating hypoalbuminemia |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C41 | Transfer of patent application or patent right or utility model | ||
| TA01 | Transfer of patent application right |
Effective date of registration: 20061215 Address after: Xiyuan Haidian District Chinese Medicine Research Institute, 1 Xiyuan grassland street, Beijing, China Applicant after: Gao Pu Co-applicant after: Beijing Puhuitianyue Biotech Co.,Ltd. Address before: Xiyuan Haidian District Chinese Medicine Research Institute, 1 Xiyuan grassland street, Beijing, China Applicant before: Gao Pu |
|
| C41 | Transfer of patent application or patent right or utility model | ||
| TA01 | Transfer of patent application right |
Effective date of registration: 20070105 Address after: Xiyuan Haidian District Chinese Medicine Research Institute, 1 Xiyuan grassland street, Beijing, China Applicant after: Gao Pu Co-applicant after: Beijing Puhui Tianyue Biotechnology Co. Ltd. Address before: Xiyuan Haidian District Chinese Medicine Research Institute, 1 Xiyuan grassland street, Beijing, China Applicant before: Gao Pu |
|
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20080521 Termination date: 20200219 |