CN118284808A - 循环肿瘤细胞分离试剂盒、循环肿瘤细胞分离容器及循环肿瘤细胞的分离方法 - Google Patents

循环肿瘤细胞分离试剂盒、循环肿瘤细胞分离容器及循环肿瘤细胞的分离方法 Download PDF

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Publication number
CN118284808A
CN118284808A CN202280077405.8A CN202280077405A CN118284808A CN 118284808 A CN118284808 A CN 118284808A CN 202280077405 A CN202280077405 A CN 202280077405A CN 118284808 A CN118284808 A CN 118284808A
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Prior art keywords
circulating tumor
cell separation
tumor cell
blood
aqueous solution
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CN202280077405.8A
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English (en)
Chinese (zh)
Inventor
内山嵩也
神田真理香
驹井邦哉
井上智雅
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Sekisui Medical Co Ltd
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Sekisui Medical Co Ltd
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Publication of CN118284808A publication Critical patent/CN118284808A/zh
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0693—Tumour cells; Cancer cells
    • C12N5/0694—Cells of blood, e.g. leukemia cells, myeloma cells
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Rigid containers without fluid transport within
    • B01L3/5082—Test tubes per se
    • B01L3/50825—Closing or opening means, corks, bungs
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/10—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by centrifugation ; Cyclones
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/04—Cell isolation or sorting
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/02—Separating microorganisms from their culture media
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0081—Purging biological preparations of unwanted cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0693—Tumour cells; Cancer cells
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2509/00—Methods for the dissociation of cells, e.g. specific use of enzymes

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Cell Biology (AREA)
  • Oncology (AREA)
  • Sustainable Development (AREA)
  • Hematology (AREA)
  • Analytical Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Urology & Nephrology (AREA)
  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Clinical Laboratory Science (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CN202280077405.8A 2022-03-25 2022-10-17 循环肿瘤细胞分离试剂盒、循环肿瘤细胞分离容器及循环肿瘤细胞的分离方法 Pending CN118284808A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2022049652 2022-03-25
JP2022-049652 2022-03-25
PCT/JP2022/038554 WO2023181468A1 (ja) 2022-03-25 2022-10-17 循環腫瘍細胞分離キット、循環腫瘍細胞分離容器及び循環腫瘍細胞の分離方法

Publications (1)

Publication Number Publication Date
CN118284808A true CN118284808A (zh) 2024-07-02

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Family Applications (1)

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CN202280077405.8A Pending CN118284808A (zh) 2022-03-25 2022-10-17 循环肿瘤细胞分离试剂盒、循环肿瘤细胞分离容器及循环肿瘤细胞的分离方法

Country Status (6)

Country Link
US (1) US20250197810A1 (https=)
EP (1) EP4502599A1 (https=)
JP (1) JP7355473B1 (https=)
KR (1) KR20240168912A (https=)
CN (1) CN118284808A (https=)
WO (1) WO2023181468A1 (https=)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP7586593B2 (ja) * 2022-09-29 2024-11-19 積水メディカル株式会社 血液保存用組成物及び血液採取容器

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH01199158A (ja) * 1987-10-20 1989-08-10 Seitetsu Kagaku Co Ltd 赤血球保存液
WO2003019141A2 (en) * 2001-08-23 2003-03-06 Immunivest Corporation Analysis of circulating tumor cells, fragments, and debris
JP2007271388A (ja) * 2006-03-30 2007-10-18 Hidetoshi Tsuchida 血清または血漿の分離方法および血液分離管
PL3006109T3 (pl) 2009-05-15 2019-12-31 Becton, Dickinson And Company Urządzenie do rozdzielania faz gęstości
SG10201605495UA (en) * 2011-07-05 2016-09-29 Becton Dickinson Co Coagulation controlling agents and devices comprising the same
BR112014018970A8 (pt) * 2012-02-02 2017-07-11 Becton Dickinson Co Dispositivos de coleta de amostra com agentes de estabilização sanguínea
US20150148212A1 (en) * 2012-05-10 2015-05-28 Konica Minolta, Inc. Process for removing red blood cells and centrifugal tube for blood collection
WO2015046557A1 (ja) * 2013-09-30 2015-04-02 積水メディカル株式会社 循環腫瘍細胞濃縮分離デバイス及び循環腫瘍細胞の濃縮分離方法
DE112017007639T5 (de) * 2017-06-13 2020-06-04 Chung Chin SUN Neues verfahren zum blutplasmaprotein-aktivitätserhalt
CN115119829B (zh) * 2017-10-19 2024-07-12 斯特雷克股份有限公司 用于胞外囊泡的溶血和凝血调节以及稳定化的组合物
JP7036670B2 (ja) * 2018-05-31 2022-03-15 アークレイ株式会社 血液中の稀少細胞検査、該検査ための血液処理方法及び採血管
CN114340493B (zh) * 2019-12-05 2025-12-23 积水医疗株式会社 血液采集容器和血浆的分离方法
US20230090675A1 (en) * 2020-03-11 2023-03-23 Sekisui Medical Co., Ltd. Leukocyte concentration separation device, blood collection container, and method for separating leukocytes

Also Published As

Publication number Publication date
EP4502599A1 (en) 2025-02-05
JP7355473B1 (ja) 2023-10-03
KR20240168912A (ko) 2024-12-02
WO2023181468A1 (ja) 2023-09-28
US20250197810A1 (en) 2025-06-19
JPWO2023181468A1 (https=) 2023-09-28

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