CN118130789A - Portable high-flux immunity analyzer - Google Patents
Portable high-flux immunity analyzer Download PDFInfo
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- CN118130789A CN118130789A CN202410385628.1A CN202410385628A CN118130789A CN 118130789 A CN118130789 A CN 118130789A CN 202410385628 A CN202410385628 A CN 202410385628A CN 118130789 A CN118130789 A CN 118130789A
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- 230000036039 immunity Effects 0.000 title abstract description 5
- 238000001514 detection method Methods 0.000 claims abstract description 233
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 177
- 230000003287 optical effect Effects 0.000 claims abstract description 102
- 238000012545 processing Methods 0.000 claims abstract description 34
- 238000003018 immunoassay Methods 0.000 claims description 25
- 230000001360 synchronised effect Effects 0.000 claims description 18
- 230000005284 excitation Effects 0.000 claims description 9
- 239000002184 metal Substances 0.000 claims description 3
- 238000000034 method Methods 0.000 abstract description 12
- 230000004907 flux Effects 0.000 abstract description 5
- 230000008569 process Effects 0.000 abstract description 5
- 238000004587 chromatography analysis Methods 0.000 abstract description 4
- 238000010586 diagram Methods 0.000 description 10
- 238000010166 immunofluorescence Methods 0.000 description 4
- 230000009471 action Effects 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- 230000006978 adaptation Effects 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 238000006073 displacement reaction Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000003908 quality control method Methods 0.000 description 2
- 230000005540 biological transmission Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000004891 communication Methods 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000008105 immune reaction Effects 0.000 description 1
- 238000003317 immunochromatography Methods 0.000 description 1
- 230000004807 localization Effects 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 230000002093 peripheral effect Effects 0.000 description 1
- 230000000750 progressive effect Effects 0.000 description 1
- 230000002035 prolonged effect Effects 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 238000012795 verification Methods 0.000 description 1
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Abstract
The application discloses a portable high-flux immunity analyzer, which relates to the technical field of chromatography, and comprises: the reagent card slot is detachably connected with the reagent card, one end of the reagent card is provided with a sample adding hole, the other end of the reagent card is provided with a plurality of detection areas, light spots emitted by the optical detection module only cover one detection area, the driving assembly is used for driving the reagent card slot and/or the optical detection module to move, so that the light spots emitted by the optical detection module sequentially scan the detection areas, the signal processing board is respectively connected with the optical detection module and the main control board, and the signal processing board processes signals fed back by the optical detection module to obtain detection results; wherein one or more detection areas correspond to a detection item. The driving assembly is used for driving the reagent clamping groove and/or the optical detection module to move, so that light spots emitted by the optical detection module sequentially scan a plurality of detection areas arranged on the reagent card, a plurality of item detections by one reagent card are realized, the detection is high in flux, the volume of the analyzer is prevented from being too large, and the on-site detection is facilitated.
Description
Technical Field
The application relates to the technical field of chromatography, in particular to a portable high-flux immunoassay instrument.
Background
The immunochromatography rapid detection technology is established in a chromatography technology and is characterized in that an antigen-antibody immunological reaction and a chromatography reaction are applied. The method has the advantages of simple detection method, high detection speed, small sample amount and the like, and can be widely applied to on-site quantitative detection.
In the traditional high-flux immunoassay analyzer, an immunofluorescence reagent card matched with a motor drive scan is used for emitting excitation light in the scanning process, and a photoelectric sensor receives excited fluorescence with specific wavelength to obtain the fluorescence intensity of a test strip CT line (a detection line T and a quality control line C) in the immunofluorescence reagent card, so that the information such as the concentration of a measurement target object is analyzed. The immunofluorescence reagent card is generally a single-card single-item detection item, and is at most a double-card detection item, and a plurality of reagent cards are used for more than two items. In order to improve the test flux, the number of reagent cards or the number of parallel immunofluorescence reagent cards is generally increased, and the whole structure of the analyzer adopting the method is complex and huge, which is more unfavorable for field detection.
Therefore, how to realize one reagent card to perform multiple project detection, and simultaneously avoid the overlarge size of the analyzer, so that the field detection is convenient, and the problem to be solved by the person skilled in the art is solved.
Disclosure of Invention
The application aims to provide a portable high-flux immunity analyzer which is used for solving the problem that the existing analyzer only detects single-card single-item or detects double-card single-item.
In order to solve the above technical problems, the present application provides a portable high-throughput immunoassay analyzer, comprising: the device comprises a reagent clamping groove, a reagent card, an optical detection module, a driving assembly, a main control board and a signal processing board;
The reagent card slot is detachably connected with the reagent card, one end of the reagent card is provided with a sample adding hole, the other end of the reagent card is provided with a plurality of detection areas, light spots emitted by the optical detection module only cover one detection area, the driving assembly is connected with the reagent card slot and/or the optical detection module and is used for driving the reagent card slot and/or the optical detection module to move so that the light spots emitted by the optical detection module sequentially scan the detection areas, the signal processing board is respectively connected with the optical detection module and the main control board, and the signal processing board is used for processing signals fed back by the optical detection module to obtain detection results; wherein one or more of the detection zones corresponds to a detection item.
Optionally, the detection areas are distributed at intervals along the x-axis direction and the y-axis direction, the driving assembly comprises a first driving assembly and a second driving assembly, the first driving assembly is connected with the reagent clamping groove, the first driving assembly is used for driving the reagent clamping groove to move along the y-axis direction, the second driving assembly is connected with the optical detection module, and the second driving assembly is used for driving the optical detection module to move along the x-axis direction.
Optionally, the first drive assembly includes first moving motor, first synchronizing wheel and connects respectively first moving motor with the first hold-in range of first synchronizing wheel, the second drive assembly includes second moving motor, second synchronizing wheel and connects respectively the second moving motor with the second hold-in range of second synchronizing wheel, reagent draw-in groove with first hold-in range is connected, optical detection module with the second hold-in range is connected.
Optionally, the optical detection module comprises an LED lamp, a slit component, a dichroic mirror, a lens, an optical filter and a photosensor; the slit component is arranged between the LED lamp and the dichroic mirror, the dichroic mirror is obliquely arranged between the lens and the optical filter, the photoelectric sensor is connected with the signal processing board, excitation light generated by the LED lamp sequentially passes through the slit of the slit component, the dichroic mirror and the lens to irradiate a detection area of the reagent card, emission light of the detection area sequentially passes through the lens, the dichroic mirror, the optical filter and the photoelectric sensor, the photoelectric sensor is used for converting an optical signal into an electric signal, and the signal processing board is used for processing the electric signal to obtain a detection result.
Optionally, the reagent draw-in groove with reagent card grafting, just reagent draw-in groove top open-ended both sides are equipped with the preforming, elastic component is located to the bottom surface in the reagent draw-in groove, elastic component is used for with reagent card with preforming butt.
Optionally, the bottom surface in the reagent draw-in groove is equipped with the locating hole, the locating hole cooperates with the locating pin in order to fix a position the reagent card, detection sensor locates the one end of reagent draw-in groove is used for detecting whether the reagent card clamps in place.
Optionally, the detection sensor includes a magnet and a hall sensor, the magnet is slidably connected in the reagent card slot, and after the reagent card is put in place, the magnet is pushed to be close to the hall sensor.
Optionally, one end of the reagent card is further provided with a plurality of positioning points, and the light spot emitted by the optical detection module only covers one positioning point.
Optionally, the reagent draw-in groove side is equipped with the connecting hole, the connecting hole passes through the sheet metal component and is connected with first synchronous area, optical detection module passes through the support to be fixed on the second synchronous area, the below of reagent draw-in groove be equipped with the slide rail and with slide rail sliding connection's slider, the slider with the bottom of reagent draw-in groove is connected.
Optionally, the device further comprises a y-axis origin sensor and an x-axis origin sensor, wherein the x-axis origin sensor is arranged at an initial position of the optical detection module in the x-axis direction, and the y-axis origin sensor is arranged at an initial position of the reagent clamping groove in the y-axis direction.
The application provides a portable high-flux immunity analyzer, which comprises: the device comprises a reagent clamping groove, a reagent card, an optical detection module, a driving assembly, a main control board and a signal processing board; the reagent card slot is detachably connected with the reagent card, one end of the reagent card is provided with a sample adding hole, the other end of the reagent card is provided with a plurality of detection areas, light spots emitted by the optical detection module only cover one detection area, the driving assembly is connected with the reagent card slot and/or the optical detection module, the driving assembly is used for driving the reagent card slot and/or the optical detection module to move so that the light spots emitted by the optical detection module sequentially scan the detection areas, the signal processing board is respectively connected with the optical detection module and the main control board, and the signal processing board is used for processing signals fed back by the optical detection module to obtain detection results; wherein one or more detection areas correspond to a detection item. Through setting up a plurality of detection areas on the reagent card, combine the removal of drive assembly drive reagent draw-in groove and/or optical detection module to make the facula that optical detection module launched scan a plurality of detection areas in proper order, thereby realize that a reagent card carries out a plurality of project and detects, detect high flux, improve detection efficiency, also avoid the volume of analysis appearance too big simultaneously, the on-the-spot detection of being convenient for.
In addition, the light source with small volume and low power consumption; the analyzer is miniaturized, compact in structure, convenient for on-site detection and suitable for various occasions.
Drawings
For a clearer description of embodiments of the present application, the drawings that are required to be used in the embodiments will be briefly described, it being apparent that the drawings in the following description are only some embodiments of the present application, and other drawings may be obtained according to the drawings without inventive effort for those skilled in the art.
FIG. 1 is a block diagram of a portable high throughput immunoassay device according to an embodiment of the present application;
FIG. 2 is a schematic diagram of a first reagent card according to an embodiment of the present application;
FIG. 3 is a schematic diagram of a second reagent card according to an embodiment of the present application;
FIG. 4 is a schematic diagram of a reagent card slot according to an embodiment of the present application;
FIG. 5 is a block diagram of an optical detection module according to an embodiment of the present application;
FIG. 6 is a schematic view of the appearance of a first portable high-throughput immunoassay device according to an embodiment of the present application;
FIG. 7 is a schematic view of the appearance of a second portable high-throughput immunoassay device according to an embodiment of the present application;
FIG. 8 is an exploded view of a portable high throughput immunoassay device according to an embodiment of the present application;
The reference numerals are as follows: 1 is a reagent card slot, 2 is a reagent card, 3 is an optical detection module, 4 is a driving component, 5 is an x-axis origin sensor, 6 is a y-axis origin sensor, 7 is a main control board, 8 is a signal processing board, 9 is a touch screen, 10 is an instrument shell, 101 is a pressing sheet, 102 is an elastic component, 103 is a positioning hole, 104 is a connecting hole, 201 is a sample adding hole, 202 is a detection area, 203 is a positioning point, 204 is a window area, 301 is an LED lamp, 302 is a slit component, 303 is a dichroic mirror, 304 is a lens, 305 is a filter, 306 is a photoelectric sensor, 401 is a first moving motor, 402 is a first synchronous belt, 403 is a first synchronous wheel, 404 is a second moving motor, 405 is a second synchronous belt, and 406 is a second synchronous wheel.
Detailed Description
The following description of the embodiments of the present application will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present application, but not all embodiments. Based on the embodiments of the present application, all other embodiments obtained by a person of ordinary skill in the art without making any inventive effort are within the scope of the present application.
The core of the application is to provide a portable high-flux immunoassay analyzer for realizing detection of a plurality of items by one reagent card, and meanwhile, the application avoids overlarge volume of the analyzer and is convenient for on-site detection.
In order to better understand the aspects of the present application, the present application will be described in further detail with reference to the accompanying drawings and detailed description.
Fig. 1 is a block diagram of a portable high-throughput immunoassay analyzer according to an embodiment of the present application, as shown in fig. 1, the portable high-throughput immunoassay analyzer includes: the device comprises a reagent card slot 1, a reagent card 2, an optical detection module 3, a driving assembly 4, a main control board 7 and a signal processing board 8; the reagent card slot 1 is detachably connected with the reagent card 2, one end of the reagent card 2 is provided with a sample adding hole 201, the other end of the reagent card 2 is provided with a plurality of detection areas 202, light spots emitted by the optical detection module 3 only cover one detection area 202, the driving component 4 is connected with the reagent card slot 1 and/or the optical detection module 3, the driving component 4 is used for driving the reagent card slot 1 and/or the optical detection module 3 to move so that the light spots emitted by the optical detection module 3 sequentially scan the detection areas 202, the signal processing board 8 is respectively connected with the optical detection module 3 and the main control board 7, and the signal processing board 8 is used for processing signals fed back by the optical detection module 3 to obtain detection results; wherein one or more of the detection regions 202 corresponds to a detection item.
The detachable connection mode of the reagent card slot 1 and the reagent card 2 is not particularly limited in the embodiment of the application, and a clamping mode and the like can be adopted. The reagent card 2 of the application has one end provided with the sample adding hole 201 and the other end provided with a plurality of detection areas 202, and the distribution mode of the detection areas 202 can be set according to the moving route of the optical detection module 3 and/or the reagent card slot 1 driven by the driving component 4, so that the light spots emitted by the optical detection module 3 scan each detection area 202 in turn, thereby realizing the detection of a plurality of items by one reagent card 2. The driving assembly 4 of the embodiment of the application is not limited to synchronous belts and synchronous wheels, gears, lead screws, screw rods and the like, and can achieve the same effect. It should be noted that, the light spot emitted by the optical detection module 3 covers only one detection area 202, and does not cover other detection areas 202 around the detection area 202, so as to avoid affecting the detection result.
The embodiment of the application provides a portable high-flux immunoassay analyzer, which comprises: the device comprises a reagent clamping groove, a reagent card, an optical detection module, a driving assembly, a main control board and a signal processing board; the reagent card slot is detachably connected with the reagent card, one end of the reagent card is provided with a sample adding hole, the other end of the reagent card is provided with a plurality of detection areas, light spots emitted by the optical detection module only cover one detection area, the driving assembly is connected with the reagent card slot and/or the optical detection module, the driving assembly is used for driving the reagent card slot and/or the optical detection module to move so that the light spots emitted by the optical detection module sequentially scan the detection areas, the signal processing board is respectively connected with the optical detection module and the main control board, and the signal processing board is used for processing signals fed back by the optical detection module to obtain detection results; wherein one or more detection areas correspond to a detection item. Through setting up a plurality of detection areas on the reagent card, combine the removal of drive assembly drive reagent draw-in groove and/or optical detection module to make the facula that optical detection module launched scan a plurality of detection areas in proper order, thereby realize that a reagent card carries out a plurality of project and detects, detect high flux, improve detection efficiency, also avoid the volume of analysis appearance too big simultaneously, the on-the-spot detection of being convenient for.
In addition, the light source with small volume and low power consumption; the analyzer is miniaturized, compact in structure, convenient for on-site detection and suitable for various occasions.
Based on the above embodiment, fig. 2 is a schematic diagram of a first reagent card provided in the embodiment of the present application, fig. 3 is a schematic diagram of a second reagent card provided in the embodiment of the present application, as shown in fig. 2 and fig. 3, in the embodiment of the present application, a plurality of detection areas 202 are distributed at intervals along an x-axis direction and a y-axis direction, and a driving assembly 4 includes a first driving assembly and a second driving assembly, where the first driving assembly is connected to the reagent card slot 1, the first driving assembly is used for driving the reagent card slot 1 to move along the y-axis direction, the second driving assembly is connected to the optical detection module 3, and the second driving assembly is used for driving the optical detection module 3 to move along the x-axis direction.
As shown in fig. 2 and fig. 3, the detection areas 202 are arranged in parallel in a plurality of rows and a plurality of columns in the window area 204, one end of the window area 204 is provided with a sample adding hole 201, the other end of the window area 204 is provided with a plurality of positioning points 203, the positioning points 203 are used for detecting whether the test paper position of the reagent card 2 is accurate, and in addition, attention is paid to the size design of the positioning points 203, so that the light spot emitted by the optical detection module 3 only covers one positioning point 203. The detection area and the localization point may be arranged as small boxes or dots. The plurality of detection regions 202 corresponds to one detection item, and in the following description, taking two detection regions 202 as an example, two detection regions in a rectangular frame in fig. 2 and 3 correspond to one detection item, two detection regions 202 corresponding to the same detection item in fig. 2 are arranged along the y-axis direction, two detection regions 202 corresponding to the same detection item in fig. 3 are arranged along the x-axis direction, and the arrangement manner of the plurality of detection regions 202 corresponding to one detection item is not particularly limited. One detection item corresponds to a plurality of detection areas 202, and is mainly used for detecting requirements, wherein one detection area 202 is a quality control point, the other detection area 202 is a detection point, and a detection result is calculated through the emitted light intensity; one detection area 202 is a detection point and the other detection area 202 is a verification point, and whether the item is effective or not is judged through comparison of the intensity of the emitted light of the detection point and the emitted light of the other detection area 202; one detection area 202 is a detection point a and the other detection area 202 is a detection point B, and the detection result is calculated by the average value of the emitted light of both detection points.
The number of the positioning points 203 and the detection areas 202 in the window area 204 can be arranged according to the requirement, and the detection items can be increased or reduced by adjusting the size of the window area 204, so that one-card multi-item detection items can be realized, the optical detection module 3 and the reagent card slot 1 can alternately move, and each detection area 202 of the reagent card 2 can be independently scanned and analyzed.
Compared with the detection area 202 only distributed along the x-axis direction, one driving component 4 only drives the reagent card slot 1 to move along the x-axis direction, in the embodiment of the application, the detection area 202 is distributed at intervals along the x-axis direction and the y-axis direction, and correspondingly, the first driving component and the second driving component respectively drive the reagent card slot 1 to move along the y-axis direction and the optical detection module 3 to move along the x-axis direction, so that the number of detection items in the reagent card 2 with limited size is improved.
Based on the above embodiment, as shown in fig. 1, the first driving assembly of the embodiment of the present application includes a first moving motor 401, a first synchronizing wheel 403, and a first synchronizing belt 402 respectively connected to the first moving motor 401 and the first synchronizing wheel 403, and the second driving assembly includes a second moving motor 404, a second synchronizing wheel 406, and a second synchronizing belt 405 respectively connected to the second moving motor 404 and the second synchronizing wheel 406, the reagent card slot 1 is connected to the first synchronizing belt 402, and the optical detection module 3 is connected to the second synchronizing belt 405.
The connection mode of the reagent card slot 1 and the first timing belt 402 and the connection mode of the optical detection module 3 and the second timing belt 405 are not limited in the embodiment of the present application. Specifically, fig. 4 is a schematic diagram of a reagent card slot provided in an embodiment of the present application, as shown in fig. 4, a connecting hole 104 is provided on a side surface of the reagent card slot 1, the connecting hole 104 is connected with a first synchronous belt 402 through a sheet metal part, an optical detection module 3 is fixed on a second synchronous belt 405 through a bracket, a sliding rail and a sliding block slidingly connected with the sliding rail are provided below the corresponding reagent card slot 1, and the sliding block is connected with the bottom of the reagent card slot 1. Further, the device further comprises a y-axis origin sensor 6 and an x-axis origin sensor 5, wherein the x-axis origin sensor 5 is arranged at an initial position of the optical detection module 3 in the x-axis direction, and the y-axis origin sensor 6 is arranged at an initial position of the reagent cartridge 1 in the y-axis direction.
The reagent card slot 1 moves in the y-axis direction, the reagent card slot 1 is fixed on the first synchronous belt 402, and the synchronous wheel and the synchronous belt convert the rotation of the motor into linear motion by taking the linear sliding rail as a guide. By adopting the direct current motor with the reduction gear box, the reagent card 2 can bear certain thrust without displacement when being inserted into the reagent card slot 1. The optical detection module 3 moves in the x-axis direction, the optical detection module 3 is fixed on the second synchronous belt 405 through a bracket, and the synchronous wheel and the synchronous belt convert the rotation of the motor into linear motion.
The operation of the first drive assembly and the second drive assembly is described below. After the instrument is started, the y-axis origin sensor 6 and the x-axis origin sensor 5 respectively detect whether the reagent clamping groove 1 and the optical detection module 3 are at initial positions. When the reagent card 2 is put into the reagent card slot 1 for detection, the reagent card slot 1 and the reagent card 2 move together to the inside of the instrument, and stop running when moving to a specified position, at this time, the optical detection module 3 completes scanning of a row of detection areas 202, then the reagent card slot 1 and the reagent card 2 immediately return to the initial position, and at the same time, the optical detection module 3 moves to the opposite direction of the initial position for a specified distance, and the distance corresponds to the interval of the detection areas 202 on the reagent card 2. The above procedure is repeated until the reagent card 2 has been scanned for all the detection areas 202, and the reagent card well 1 and the optical detection module 3 remain in the initial positions. When the scanning flow of the reagent card 2 is carried out, the light source in the optical detection module 3 is turned on, and when the reagent card 2 returns to the initial position, the light source in the optical detection module 3 is turned off, so that the service life of the light source can be prolonged, and the power consumption of the instrument can be reduced. When the scanning flow of the reagent card 2 is carried out, the running speed of the reagent card slot 1 is kept at a constant speed, and when the scanning is finished and the reagent card slot 1 returns to the initial position, the running speed of the reagent card slot 1 can be higher than the scanning speed, the whole time for returning to the initial position is shortened, and the detection speed of an instrument is improved.
Based on the above embodiments, fig. 5 is a block diagram of an optical detection module provided in the embodiment of the present application, and as shown in fig. 5, an optical detection module 3 in the embodiment of the present application includes an LED lamp 301, a slit component 302, a dichroic mirror 303, a lens 304, an optical filter 305, and a photoelectric sensor 306; the slit component 302 is arranged between the LED lamp 301 and the dichroic mirror 303, the dichroic mirror 303 is obliquely arranged between the lens 304 and the optical filter 305, the photoelectric sensor 306 is connected with the signal processing board 8, excitation light generated by the LED lamp 301 sequentially irradiates the detection area 202 of the reagent card 2 through the slit of the slit component 302, the dichroic mirror 303 and the lens 304, emission light of the detection area 202 sequentially passes through the lens 304, the dichroic mirror 303, the optical filter 305 and the photoelectric sensor 306, the photoelectric sensor 306 is used for converting optical signals into electric signals, and the signal processing board 8 is used for processing the electric signals to obtain detection results.
The optical detection module 3 in the embodiment of the application can simplify the whole light path at the premise of ensuring the excitation light intensity of the LED lamp 301, and the light spot of the excitation light is matched with the matched reagent card 2. The excitation light spot presents a short straight-line light spot in the window area 204 of the reagent card 2, the light spot just can cover one detection area 202 on the reagent card 2, and the peripheral detection area 202 is not in the light spot range. The optical detection module 3 realizes the scanning of the detection areas 202 in the window area 204 of the reagent card 2 one by one through the first driving component and the second driving component, but not the simultaneous scanning of all detection items in the whole window area 204 of the reagent card 2. The scanning method is not limited to column-by-column scanning or line-by-line scanning. The optical detection module 3 has compact integral structure, greatly controls the integral overall dimension of the optical detection module 3 under the condition of ensuring the light intensity of an excitation light source, and can be applied to various portable high-flux fluorescence analyzers. The excitation light spot is miniaturized, and the high-precision driving assembly is combined with a plurality of reagent cards to realize the detection of a plurality of items of reagent cards with the same size, so that the portable and miniaturized fluorescence analyzer achieves high flux.
Based on the above embodiment, as shown in fig. 4, in the embodiment of the present application, the reagent card slot 1 is inserted into the reagent card 2, and the pressing pieces 101 are disposed on two sides of the top opening of the reagent card slot 1, the bottom surface in the reagent card slot 1 is disposed on the elastic member 102, and the elastic member 102 is used for abutting the reagent card 2 against the pressing pieces 101. The embodiment of the present application is not particularly limited, and the elastic member 102 may be a spring, and the elastic member 102 is used to abut the reagent card 2 against the pressing piece 101, and fix the reagent card 2 so that no displacement occurs during detection.
Further, the bottom surface in the reagent card slot 1 is equipped with locating hole 103 and detection sensor, and locating hole 103 cooperates with the locating pin in order to realize the location of reagent card 2, and detection sensor locates the one end of reagent card slot 1 for detect whether reagent card 2 clamps in place. The positioning pins are abutted against the two sides of the reagent card 2 to play a role in positioning, so that the optical detection module 3 is not influenced by the detection area 202 beside when scanning one of the detection areas 202. The embodiment of the application does not limit the detection sensor specifically, and specifically, the detection sensor comprises a magnet and a Hall sensor, the magnet is connected in the reagent card slot 1 in a sliding way, and after the reagent card 2 is put in place, the magnet is pushed to be close to the Hall sensor. The detection sensor is preferably a Hall sensor, and the reagent card slot 1 is higher in reliability without considering wire harness connection when moving, wherein a magnet for sensing the Hall sensor is arranged in the reagent card slot 1, and the magnet is close to the Hall sensor after the reagent card 2 is arranged in place, so that the purpose of detecting the reagent card 2 is achieved.
Based on the above embodiments, fig. 6 is an external schematic view of a first portable high-throughput immunoassay device according to an embodiment of the present application; FIG. 7 is a schematic view of the appearance of a second portable high-throughput immunoassay device according to an embodiment of the present application; fig. 8 is an exploded view of a portable high-throughput immunoassay device according to an embodiment of the present application. As shown in fig. 6, the main control board 7, the reagent card slot 1, the optical detection module 3, the driving component 4, the signal processing board 8 and the battery pack are integrated in the instrument shell 10, the outer wall of the instrument shell 10 is provided with the touch screen 9, the power switch and the data interface, and the configuration can ensure that the instrument has compact structure and minimized volume under the condition of complete functions. As shown in fig. 7, the main control board 7, the reagent card slot 1, the optical detection module 3, the driving assembly 4 and the signal processing board 8 are integrated in the instrument housing 10, and the built-in communication module (bluetooth or WIFI module) is connected with a mobile phone or a computer, and the operation and data transmission of the analyzer are performed through the mobile phone or the computer, so that the overall structure of the analyzer is minimized.
The portable high-flux immunoassay analyzer provided by the application is described in detail above. In the description, each embodiment is described in a progressive manner, and each embodiment is mainly described by the differences from other embodiments, so that the same similar parts among the embodiments are mutually referred. It should be noted that it will be apparent to those skilled in the art that various modifications and adaptations of the application can be made without departing from the principles of the application and these modifications and adaptations are intended to be within the scope of the application as defined in the following claims.
It should also be noted that in this specification, relational terms such as first and second, and the like are used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Moreover, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus. Without further limitation, an element defined by the phrase "comprising one … …" does not exclude the presence of other like elements in a process, method, article, or apparatus that comprises the element.
Claims (10)
1. A portable high throughput immunoassay analyzer comprising: the device comprises a reagent clamping groove (1), a reagent card (2), an optical detection module (3), a driving assembly (4), a main control board (7) and a signal processing board (8);
The reagent card slot (1) is detachably connected with the reagent card (2), one end of the reagent card (2) is provided with a sample adding hole (201), the other end of the reagent card (2) is provided with a plurality of detection areas (202), light spots emitted by the optical detection module (3) only cover one detection area (202), the driving component (4) is connected with the reagent card slot (1) and/or the optical detection module (3), the driving component (4) is used for driving the reagent card slot (1) and/or the optical detection module (3) to move so that the light spots emitted by the optical detection module (3) sequentially scan the detection areas (202), the signal processing board (8) is respectively connected with the optical detection module (3) and the main control board (7), and the signal processing board (8) is used for processing signals fed back by the optical detection module (3) so as to obtain detection results;
wherein one or more of the detection zones (202) corresponds to a detection item.
2. The portable high-throughput immunoassay analyzer according to claim 1, wherein a plurality of the detection zones (202) are spaced apart along an x-axis direction and a y-axis direction, the driving assembly (4) comprises a first driving assembly and a second driving assembly, the first driving assembly is connected with the reagent card slot (1), the first driving assembly is used for driving the reagent card slot (1) to move along the y-axis direction, the second driving assembly is connected with the optical detection module (3), and the second driving assembly is used for driving the optical detection module (3) to move along the x-axis direction.
3. The portable high-throughput immunoassay analyzer according to claim 1, wherein the first driving assembly comprises a first moving motor (401), a first synchronizing wheel (403) and a first synchronizing belt (402) respectively connected with the first moving motor (401) and the first synchronizing wheel (403), the second driving assembly comprises a second moving motor (404), a second synchronizing wheel (406) and a second synchronizing belt (405) respectively connected with the second moving motor (404) and the second synchronizing wheel (406), the reagent clamping groove (1) is connected with the first synchronizing belt (402), and the optical detection module (3) is connected with the second synchronizing belt (405).
4. The portable high-throughput immunoassay analyzer of claim 1, wherein the optical detection module (3) comprises an LED lamp (301), a slit member (302), a dichroic mirror (303), a lens (304), a filter (305), and a photosensor (306); the slit component (302) is arranged between the LED lamp (301) and the dichroic mirror (303), the dichroic mirror (303) is obliquely arranged between the lens (304) and the optical filter (305), the photoelectric sensor (306) is connected with the signal processing board (8), excitation light generated by the LED lamp (301) sequentially passes through the slit of the slit component (302), the dichroic mirror (303) and the lens (304) to irradiate the detection area (202) of the reagent card (2), emission light of the detection area (202) sequentially passes through the lens (304), the dichroic mirror (303), the optical filter (305) and the photoelectric sensor (306), the photoelectric sensor (306) is used for converting optical signals into electric signals, and the signal processing board (8) is used for processing the electric signals to obtain detection results.
5. The portable high-throughput immunoassay analyzer according to claim 1, wherein the reagent card slot (1) is inserted into the reagent card (2), two sides of the top opening of the reagent card slot (1) are provided with pressing pieces (101), the bottom surface in the reagent card slot (1) is provided with an elastic component (102), and the elastic component (102) is used for abutting the reagent card (2) against the pressing pieces (101).
6. The portable high-throughput immunoassay analyzer according to claim 5, wherein a positioning hole (103) is provided at the bottom surface in the reagent card slot (1), the positioning hole (103) cooperates with a positioning pin to position the reagent card (2), and a detection sensor is provided at one end of the reagent card slot (1) for detecting whether the reagent card (2) is clamped in place.
7. The portable high-throughput immunoassay instrument of claim 6, wherein said detection sensor comprises a magnet and a hall sensor, said magnet being slidably coupled within said reagent card slot (1), said magnet being urged toward said hall sensor after said reagent card (2) is loaded in place.
8. Portable high-throughput immunoassay analyzer according to claim 1, characterized in that one end of the reagent card (2) is further provided with a plurality of anchor points (203), and the light spot emitted by the optical detection module (3) covers only one of the anchor points (203).
9. The portable high-throughput immunoassay analyzer according to claim 3, wherein a connecting hole (104) is formed in the side face of the reagent clamping groove (1), the connecting hole (104) is connected with the first synchronous belt (402) through a sheet metal part, the optical detection module (3) is fixed on the second synchronous belt (405) through a bracket, a sliding rail and a sliding block in sliding connection with the sliding rail are arranged below the reagent clamping groove (1), and the sliding block is connected with the bottom of the reagent clamping groove (1).
10. The portable high-throughput immunoassay analyzer according to claim 2, further comprising a y-axis origin sensor (6) and an x-axis origin sensor (5), wherein the x-axis origin sensor (5) is provided at an initial position of the optical detection module (3) in the x-axis direction, and the y-axis origin sensor (6) is provided at an initial position of the reagent cartridge (1) in the y-axis direction.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202410385628.1A CN118130789A (en) | 2024-04-01 | 2024-04-01 | Portable high-flux immunity analyzer |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202410385628.1A CN118130789A (en) | 2024-04-01 | 2024-04-01 | Portable high-flux immunity analyzer |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN118130789A true CN118130789A (en) | 2024-06-04 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202410385628.1A Pending CN118130789A (en) | 2024-04-01 | 2024-04-01 | Portable high-flux immunity analyzer |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN118130789A (en) |
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2024
- 2024-04-01 CN CN202410385628.1A patent/CN118130789A/en active Pending
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