CN220019356U

CN220019356U - Fluorescence detector

Info

Publication number: CN220019356U
Application number: CN202321498887.2U
Authority: CN
Inventors: 董薇; 肖晓月; 李宜轩; 赖成铭; 田迦楠
Original assignee: Guangzhou Yisan Electronic Technology Co ltd
Current assignee: Guangzhou Yisan Electronic Technology Co ltd
Priority date: 2023-06-13
Filing date: 2023-06-13
Publication date: 2023-11-14
Anticipated expiration: 2033-06-13

Abstract

The utility model discloses a fluorescence detector, which comprises a base and a light filtering module, wherein the base is provided with a channel, the base is provided with a first-stage fluorescence detection channel and a first-stage excitation light path channel, the light filtering module is provided with a corresponding second-stage fluorescence detection channel and a corresponding second-stage excitation light path channel, and the light filtering module is enabled to move in the channel of the base to be opposite to the first-stage fluorescence detection channel and the first-stage excitation light path channel by arranging a plurality of light filtering modules for filtering different wavelengths in the channel of the base, so that excitation light irradiation of different wavelengths is realized, fluorescent signals of different wavelengths are excited, and the detection efficiency is improved. On the basis of the convex lenses sharing light condensation or light scattering, only the optical filter is required to be installed in the optical filter module, and the optical filter module provided with the optical filters with different wavelengths is opposite to the convex lenses by moving back and forth, so that the reagent card supporting detection of different wavelengths can be realized, and the structure is simple, so that a plurality of optical filter modules can be manufactured for replacement, the installation quantity of the convex lenses is reduced, and the cost is reduced.

Description

Fluorescence detector

Technical Field

The utility model relates to the technical field of medical detection, in particular to a fluorescence detector which is convenient to apply to detecting reagent cards with different wavelengths.

Background

The fluorescent method in POCT is a method for detecting biomolecules (e.g., proteins, nucleic acids, etc.) by fluorescent labeling technique. The principle is that the fluorescent substance and the object to be detected are combined together to form the fluorescent labeling compound by utilizing the characteristic of the fluorescent substance, namely the characteristic that the fluorescent substance emits fluorescence after being excited by the excitation light with specific wavelength. The presence and concentration of the analyte is then detected quantitatively or qualitatively by measuring the intensity of the fluorescent signal in the sample. In a fluorescence spectrometer, a sample is irradiated by an excitation light source of a specific wavelength, and a fluorescent signal of the specific wavelength is emitted from an analyte and a fluorescent label complex. The magnitude of the fluorescent signal at this wavelength is proportional to the concentration of the analyte, and thus the concentration of the analyte can be quantitatively or qualitatively detected by measuring the fluorescent signal.

The fluorescence labeling compound adopted by the reagents of different manufacturers is different, so that the adopted excitation light wavelength and the fluorescence wavelength after excitation are different, and the common wavelengths of the activating light and the fluorescence signal in the market are as follows: 470/525nm, 365/610nm. The current fluorescence detection instrument on the market can only detect a group of wavelengths of one type of reagent card, for example, publication number CN216646264U, the patent name is an optical path frame for a fluorescence detector, and the structure inserts a lens, an optical filter and a dichroic mirror on a fluorescence channel, if reagent cards with different wavelengths are to be detected, different optical filters are required to be replaced, so that the cost and time are consumed, and the detection efficiency is not improved.

Currently, there is a fluorescence detector capable of supporting 2 wavelength reagent cards, for example, publication No. CN 210775218U, entitled a parallel fluorescence detector with different wavelengths, the detection module of the structure includes two housings and at least one installer, the installer is arranged between the two housings in parallel, one side of the housing is formed with a light path structure, two sides of the installer are respectively formed with a light path structure, a light path module is arranged between the housing and the adjacent installer, a light path module is arranged between the two adjacent installers, the light path module includes an excitation component and a color filter component, the excitation component includes a light source, a collimating lens, a first filter, a dichroic mirror and a first plano-convex lens, the color filter component includes a second filter, a second plano-convex lens and a light shielding plate, although the requirement of supporting fluorescence detection of 2 wavelength reagent cards is satisfied, the fluorescence detector adopts 2 sets of detection light paths, the structure is complex, the installed lenses and the filters are more, the cost and the volume of the instrument are high.

Disclosure of Invention

The utility model provides the fluorescence detector which has a simple structure and is convenient for detecting reagent cards with different wavelengths.

The fluorescence detector comprises a base and a plurality of filtering modules which are positioned in the base and used for filtering different wavelengths, wherein a first-stage fluorescence detection channel penetrating through the base and a first-stage excitation light path channel penetrating through the middle of the first-stage fluorescence detection channel from one side of the base are arranged on the base, the filtering modules comprise a mounting block body, a second-stage fluorescence detection channel penetrating through the mounting block body and a second-stage excitation light path channel penetrating through the middle of the second-stage fluorescence detection channel from one side of the mounting block body are arranged on the mounting block body, a first filter and a dichroic mirror which is obliquely arranged from top to bottom and is opposite to the second-stage excitation light path channel are arranged on the second-stage fluorescence detection channel, a second filter is arranged on the second-stage excitation light path channel, a channel which is opposite to the second-stage fluorescence detection channel is arranged on the middle of the base, a first convex lens used for scattering light is arranged on the first-stage excitation light path channel, and a second convex lens and a third convex lens used for focusing light are respectively arranged on the upper side and the lower side of the first-stage fluorescence detection channel.

By adopting the technical scheme, the utility model can obtain the following technical effects:

the light filtering modules for filtering different wavelengths are arranged in the channels, so that the light filtering modules move in the channels of the base to be opposite to the primary fluorescence detection channels and the primary excitation light path channels, excitation light irradiation of different wavelengths is realized, fluorescence signals of different wavelengths are excited, and detection efficiency is improved. The utility model has simple structure, and on the basis of the convex lens sharing light condensation or light scattering, only the filter is arranged in the filter module, and the filter module provided with the filters with different wavelengths is opposite to the convex lens by moving back and forth, so that the reagent card supporting detection of different wavelengths can be realized, and the structure is simple, thereby a plurality of filter modules can be manufactured for replacement, the installation quantity of the convex lens is reduced, and the cost is reduced.

Drawings

FIG. 1 is a diagram showing a state of detection of a fluorescence detector.

FIG. 2 is a second state diagram of the fluorescence detector.

FIG. 3 is a schematic diagram of a fluorescence detector.

Fig. 4 is a schematic view of a base structure.

Fig. 5 is a schematic diagram of a structure of a filter module.

FIG. 6 is a schematic diagram of a driving structure for driving the reagent card to move.

Description of the embodiments

The following description of the embodiments of the present utility model will be made clearly and fully with reference to the accompanying drawings, in which it is evident that the embodiments described are only some, but not all embodiments of the utility model. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.

As shown in fig. 1-6, a fluorescence detector comprises a base 1 and a plurality of filtering modules 2 which are positioned in the base and used for filtering different wavelengths, wherein a first-stage fluorescence detection channel 101 penetrating through the base and a first-stage excitation light path channel 102 which penetrates through the middle part of the first-stage fluorescence detection channel from one side of the base are arranged on the base, the filtering modules 2 comprise a mounting block body 201, a second-stage fluorescence detection channel 202 penetrating through the mounting block body and a second-stage excitation light path channel 203 which penetrates through the middle part of the second-stage fluorescence detection channel from one side of the mounting block body are arranged on the mounting block body, a first filter 3 and a dichroic mirror 4 which is obliquely arranged from top to bottom and is opposite to the second-stage excitation light path channel are arranged on the second-stage fluorescence detection channel, a channel 5 is arranged in the middle part of the base, a channel 6 which is used for enabling the first-stage fluorescence detection channel to be opposite to the second-stage fluorescence detection channel and a first-stage excitation light path channel to be opposite to the second-stage excitation light path channel is arranged on the mounting block body, a first convex lens 7 used for light scattering is arranged on the first-stage fluorescence detection channel, and second convex lenses 8 and third convex lenses 8 and 9 are respectively arranged on the upper and lower sides of the first-stage fluorescence detection channel and lower sides of the channel are respectively arranged on the channel. The light filtering modules for filtering different wavelengths are arranged in the channels, so that the light filtering modules move in the channels of the base to be opposite to the primary fluorescence detection channels and the primary excitation light path channels, excitation light irradiation of different wavelengths is realized, fluorescence signals of different wavelengths are excited, and detection efficiency is improved. The utility model has simple structure, and on the basis of the convex lens sharing light condensation or light scattering, only the filter is arranged in the filter module, and the filter module provided with the filters with different wavelengths is opposite to the convex lens by moving back and forth, so that the reagent card supporting detection of different wavelengths can be realized, and the structure is simple, thereby a plurality of filter modules can be manufactured for replacement, the installation quantity of the convex lens is reduced, and the cost is reduced.

A detection circuit board 10 for receiving fluorescent irradiation is arranged on the base at the outlet of the primary fluorescent detection channel. The detection circuit board for receiving fluorescence can be placed at any position at the outlet of the primary fluorescence detection channel, so that the detection circuit board can be better fixed, and the detection circuit board is arranged on the base at the outlet of the primary fluorescence detection channel.

A light source 11 for emitting light is arranged on the base at the entrance of the primary excitation light path channel. The light source can be arranged at any position of the entrance of the primary excitation light path channel, so that the light source can be better fixed, and is arranged on the base of the entrance of the primary excitation light path channel, and the light source can be an LED lamp.

The below of base is provided with the draw-in groove 13 that is used for placing reagent card 12 and drives draw-in groove back and forth movement's actuating mechanism, actuating mechanism includes first motor 14 and the first lead screw 15 that links to each other with first motor, is equipped with the nut 16 that cup joints mutually with first lead screw on the draw-in groove, and the below of draw-in groove sets up the fixing base, and the fixing base is equipped with the slide rail, and the draw-in groove is equipped with the spout with slide rail matched with. The first motor is a stepping motor, the distance for driving the clamping groove to move can be better and more accurately through the stepping motor, the reagent card on the clamping groove is more accurate and is opposite to the one-level fluorescence detection channel, the stepping motor drives the first screw rod to rotate, the clamping groove moves back and forth under the cooperation of the first screw rod and the nut, and then the position of the reagent card opposite to the one-level fluorescence detection channel moves back and forth to detect the reagent.

The second screw rod 17 in threaded connection with the optical filtering module 2 and the second motor 18 driving the second screw rod to rotate are installed in the channel, the second motor is a stepping motor, the optical filtering module moves in the channel under the driving of the second screw rod, the moving distance of the optical filtering module is controlled under the accurate control circle number of the stepping motor, and then the second fluorescent detection channel and the second excitation light path channel of the optical filtering module are respectively opposite to the first fluorescent detection channel and the first excitation light path channel, so that the optical filtering module is quickly replaced, and the excitation light wavelength and the detection fluorescent wavelength are realized.

The sliding rail is arranged in the channel, and the sliding groove matched with the sliding rail is arranged on the optical filtering module, so that the optical filtering module can move more stably in the channel.

Claims

1. The utility model provides a fluorescence detector, its characterized in that includes base (1) and a plurality of filter module (2) that are located inside the base that are used for filtering different wavelength, be equipped with on the base and run through one-level fluorescence detection passageway (101) of base and pass one-level excitation light path passageway (102) with one-level fluorescence detection passageway middle part intercommunication from base one side, filter module (2) including installation piece body (201), be equipped with on the installation piece body and run through second fluorescence detection passageway (202) of installation piece body and pass second excitation light path passageway (203) with second fluorescence detection passageway middle part intercommunication from installation piece body one side, second fluorescence detection passageway is installed first light filter (3) from top to bottom and slope setting and with second excitation light path passageway opposite two-way dichroic (4), second light filter (5) are installed to second excitation light path passageway, the middle part of base is equipped with and is used for filter module round trip movement to make one-level fluorescence detection passageway and second fluorescence detection passageway opposite, one-level excitation light path passageway and second excitation light path opposite passageway (6), one-level excitation passageway installs first convex lens (7) that are used for astigmatic, and one-level fluorescence detection passageway is located two sides convex lens (8), respectively, and convex lens (9) are located down.

2. The fluorescence detector according to claim 1, characterized in that a detection circuit board (10) for receiving fluorescent illumination is mounted on the base at the outlet of the primary fluorescence detection channel.

3. The fluorescence detector according to claim 1, characterized in that a light source (11) for emitting light is mounted on the base at the entrance of the primary excitation light path channel.

4. The fluorescence detector according to claim 1, characterized in that a clamping groove (13) for placing a reagent card (12) and a driving mechanism for driving the clamping groove to move back and forth are arranged below the base, the driving mechanism comprises a first motor (14) and a first screw rod (15) connected with the first motor, a nut (16) sleeved with the first screw rod is arranged on the clamping groove, a fixing seat is arranged below the clamping groove, a sliding rail is arranged on the fixing seat, and a sliding groove matched with the sliding rail is arranged on the clamping groove.

5. The fluorescence detector according to claim 1, characterized in that a second screw (17) in threaded connection with the filter module (2) and a second motor (18) for driving the second screw to rotate are installed in the channel.

6. The fluorescence detector of claim 1, wherein a slide rail is disposed in the channel, and a slide slot is disposed on the filter module that mates with the slide rail.