CN118120616A - Breeding method for secret-resistant mechanical-harvest corn seeds - Google Patents
Breeding method for secret-resistant mechanical-harvest corn seeds Download PDFInfo
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- 240000008042 Zea mays Species 0.000 title claims abstract description 178
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 title claims abstract description 178
- 235000002017 Zea mays subsp mays Nutrition 0.000 title claims abstract description 178
- 235000005822 corn Nutrition 0.000 title claims abstract description 178
- 238000009395 breeding Methods 0.000 title claims abstract description 51
- 238000003306 harvesting Methods 0.000 title claims abstract description 25
- 230000001488 breeding effect Effects 0.000 claims abstract description 44
- 241000196324 Embryophyta Species 0.000 claims abstract description 33
- 238000000034 method Methods 0.000 claims abstract description 23
- 238000005516 engineering process Methods 0.000 claims abstract description 12
- 238000009331 sowing Methods 0.000 claims abstract description 10
- 239000003147 molecular marker Substances 0.000 claims abstract description 5
- 239000002689 soil Substances 0.000 claims abstract description 4
- 238000011161 development Methods 0.000 claims description 18
- 239000003337 fertilizer Substances 0.000 claims description 9
- 230000010152 pollination Effects 0.000 claims description 7
- 238000000926 separation method Methods 0.000 claims description 7
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 claims description 6
- 230000015572 biosynthetic process Effects 0.000 claims description 6
- 239000004202 carbamide Substances 0.000 claims description 6
- 238000005286 illumination Methods 0.000 claims description 6
- 238000012216 screening Methods 0.000 claims description 6
- 230000009418 agronomic effect Effects 0.000 claims description 5
- 206010003591 Ataxia Diseases 0.000 claims description 4
- 206010010947 Coordination abnormal Diseases 0.000 claims description 4
- 208000016290 incoordination Diseases 0.000 claims description 4
- 208000035240 Disease Resistance Diseases 0.000 claims description 3
- 241000607479 Yersinia pestis Species 0.000 claims description 3
- WZLMXYBCAZZIRQ-UHFFFAOYSA-N [N].[P].[K] Chemical compound [N].[P].[K] WZLMXYBCAZZIRQ-UHFFFAOYSA-N 0.000 claims description 3
- 238000009825 accumulation Methods 0.000 claims description 3
- 210000005069 ears Anatomy 0.000 claims description 3
- 230000002349 favourable effect Effects 0.000 claims description 3
- 238000002955 isolation Methods 0.000 claims description 3
- 230000035800 maturation Effects 0.000 claims description 3
- 235000015097 nutrients Nutrition 0.000 claims description 3
- 239000002245 particle Substances 0.000 claims description 3
- 239000002686 phosphate fertilizer Substances 0.000 claims description 3
- 238000001556 precipitation Methods 0.000 claims description 3
- 230000000630 rising effect Effects 0.000 claims description 3
- 230000018109 developmental process Effects 0.000 description 12
- 235000009508 confectionery Nutrition 0.000 description 10
- 230000008901 benefit Effects 0.000 description 5
- 229920002472 Starch Polymers 0.000 description 4
- 239000008107 starch Substances 0.000 description 4
- 230000009286 beneficial effect Effects 0.000 description 2
- 230000007547 defect Effects 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 235000019698 starch Nutrition 0.000 description 2
- 238000012271 agricultural production Methods 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 238000007710 freezing Methods 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000002035 prolonged effect Effects 0.000 description 1
- 230000007226 seed germination Effects 0.000 description 1
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Abstract
The invention relates to the technical field of corn seed selection, in particular to a method for selecting a corn seed with high density and mechanical harvest resistance, which comprises the following steps: firstly, planting ditches, piling up soil, ridging, alternately sowing female parent and male parent on the spaced ridges, wherein the planting density is 5000-6000 plants/mu; the female parent is drought-resistant corn seeds, the male parent is drought-resistant corn seeds, the conventional breeding technology, the haploid breeding technology and the molecular marker assisted selection are combined to select and breed the breakthrough drought-resistant corn female parent and the drought-resistant corn male parent, and in the selection and breeding of the mechanical-harvest-resistant corn seeds, a proper selection strategy is adopted, so that the improvement of the selection and breeding efficiency is facilitated.
Description
Technical Field
The invention relates to the technical field of corn seed selection and evaluation, in particular to a method for selecting and breeding a corn seed with high density and suitable mechanical harvest.
Background
Corn is one of the most important food crops in the world, and has important economic and social significance for agricultural production. Corn breeding refers to optimizing the quality and yield of corn through manual selection and genetic improvement, and improving the economic benefit of farmers.
The 'high-starch corn variety breeding method' disclosed in the application number of CN114885829A is also an increasingly mature technology, and relates to the technical field of corn variety breeding, and the method comprises the following steps: s1: preparing land, forming planting ridges, alternately planting female parent and male parent on adjacent ridges, wherein the planting density is 4500-6000 plants/mu; the female parent is 0519, and the male parent is 0571; and the planting time is adjusted according to the growth cycle of the female parent and the male parent, so that the silk-laying full period of the female parent is 2-3 days earlier than the powder-scattering full period of the male parent; s2: removing tassel of the female parent before pollination period; s3: obtaining corn with female parent plant cultured to obtain high starch corn seed. The high-starch corn seeds cultivated by the method have the advantages that the starch content is improved, and the corn yield is obviously improved; the breeding method has the advantages of convenient operation, short period and high success rate; the method for breeding sweet waxy fresh corn varieties disclosed in the application number of CN114521487B is also an increasingly mature technology, and a sweet waxy corn inbred line A is created by crossing a waxy corn variety and a super sweet waxy homozygous double-hidden-backbone inbred line; and (3) hybridizing the sweet waxy corn inbred line A and the waxy corn backbone inbred line to obtain sweet waxy fresh corn varieties. The breeding method provided by the invention can create an excellent sweet glutinous inbred line, and has the advantages of high seed germination rate, good plant agronomic characters, strong resistance and high sugar content in seeds; the sugar content of the seeds of the bred sweet waxy novel fresh corn variety reaches 14%. The sweet waxy corn obtained by the breeding method has the advantages that the sugar content and the taste quality of the seeds are obviously improved compared with those of common waxy corn, the harvesting period is greatly prolonged compared with that of sweet waxy corn, and the sweet waxy corn is suitable for fresh food and quick-freezing processing.
However, the design of the breeding method has the following defects: the existing secret-resistant mechanical-harvest corn seed selection can only be used for single-double-ridge cultivation, so that the growth of corn is influenced, the accuracy of selection is influenced, and the corn selection is inaccurate.
Disclosure of Invention
Aiming at the defects of the prior art, the invention provides a method for breeding the secret-resistant mechanical-harvest corn seeds, which solves the problems in the background art.
In order to achieve the above purpose, the present invention provides the following technical solutions: a method for breeding a secret-resistant mechanical-harvest corn seed comprises the following steps:
Firstly, planting ditches, piling up soil, ridging, alternately sowing female parent and male parent on the spaced ridges, wherein the planting density is 5000-6000 plants/mu; the female parent is drought-resistant corn seeds, and the male parent is drought-resistant corn seeds;
According to the flowering-period planting time of the drought-resistant corn female parent and the drought-resistant corn male parent, the full bloom period of the drought-resistant corn female parent is 1-3 days earlier than the pollen scattering period of the drought-resistant corn male parent, corn is not required to pass through different growth and development stages from sowing to maturing, the requirements of the corn on illumination, temperature, precipitation and the like are different in the different growth and development stages, the proper planting climate conditions are required to be known in the corn growth and development, the normal growth and development of the corn is ensured, and the high-quality and high-yield formation of the corn is promoted;
Step two, in the period from seed selection and sowing of corn seeds to seedling emergence, the lowest sprouting temperature of the seeds is about 7.5 ℃, the average temperature is 25-35 ℃, the seedling emergence speed is accelerated along with the rising of the temperature in the temperature range, the average temperature in the corn seedling stage is most suitable when the average temperature is 18.5-19.5 ℃, the proper growth temperature of corn root systems is obtained when the ground temperature at 5cm is 20-25 ℃, and the seedling stage is controlled to normally grow in the weather of more than 20 ℃;
The average air temperature is about 5.6 ℃, the initial day of which the daily average air temperature is more than or equal to 10 ℃ is basically in the middle 5 months of the year, the final day is in the late 10 months, the frost free period is about 145d, the active accumulated temperature of which the temperature is more than or equal to 10 ℃ in the growing season (5-9 months) of corn crops is about 4 ℃ d, and the day-night temperature difference is more suitable, so that the corn planting and seed selection are more suitable;
step three, in the late emergence period of the corn, if the daily sunshine hours exceeds 8 hours, the time of the growth and development period of the corn is advanced; if the number of sunshine hours after emergence is less than 8 hours, the growth period time is shortened;
And fourthly, the sunshine duration in the seed selection and growth season of the corn seeds in the seed selection period of 5-9 months is about 1159 hours, the sunshine duration in6 months is longer, the method is favorable for the formation of seedlings, the illumination condition in the later stage is stronger, the normal operation and accumulation of nutrients in the later stage of grouting and maturation period of the corn can be promoted, the thousand seed weight can be increased, and the corn yield can be improved.
Step five, removing tassel of a drought-resistant corn seed female parent before the pollination period;
step six, obtaining corn of which drought-resistant corn seed female parent plants are cultured, and obtaining corn particle seeds.
Preferably, the number ratio of the drought-resistant corn female parent to the drought-resistant corn male parent in the first step is 1-3.5:1, namely 2 ridges of drought-resistant corn male parent are planted in every 2-7 ridges of drought-resistant corn female parent.
Preferably, the number ratio of the drought-resistant corn female parent to the drought-resistant corn male parent in the second step is 4:2, namely, each interval of 4 ridges of drought-resistant corn female parent is planted with 2 ridges of drought-resistant corn male parent.
Preferably, step four, the method of combining the conventional breeding technology, haploid breeding technology and molecular marker assisted selection is utilized to select and hybridize the breakthrough drought-resistant corn female parent and the drought-resistant corn male parent, and in the selection and breeding of the mechanical harvest corn seeds of the secret resistance, a proper selection strategy is adopted, so that the improvement of the selection and breeding efficiency is facilitated.
Preferably, artificial supplementary pollination is carried out, pollen of the drought-resistant corn male parent is collected and applied to the flowers of the drought-resistant corn female parent, the pollen is applied once in the morning and afternoon, the population is enlarged, the density is improved, the population quantity is increased in the generation of character separation, particularly in the most obvious S1 generation, the number of the population is increased, the number is generally more than 6000 plants/mu, the densities of the drought-resistant corn male parent and the drought-resistant corn female parent can be properly increased to 7000-8000 plants/mu, and plants and spike rows with poor agronomic characters such as incoordination between male and female are eliminated.
Preferably, in early generation of drought-resistant corn female parent and drought-resistant corn male parent breeding, as far as possible, in different ecological mountainous areas, such as islands, additional generation isolation culture breeding is performed, plants with larger ears, better resistance and strong green holding property are selected according to breeding targets, mechanical harvest quality screening is focused, pest and disease resistance screening is performed, and excellent ear rows with higher biological yield are selected.
Preferably, fertilizer is applied to the ridges during planting, wherein the fertilizer comprises 15-20 kg/mu of phosphate fertilizer and 5-10 kg/mu of urea;
in the heading stage, 50% of nitrogen-phosphorus-potassium compound fertilizer 15 kg/mu and urea 15 kg/mu are applied;
Watering for 4-6 times in the whole growth and development period.
Compared with the prior art, the invention has the beneficial effects that:
The invention provides a method for breeding a secret-resistant mechanical-harvest corn seed, which comprises the following steps:
1. the method combines the conventional breeding technology, the haploid breeding technology and the molecular marker assisted selection to select and hybridize the breakthrough drought-resistant corn female parent and the drought-resistant corn male parent, and the proper selection strategy is adopted in the selection of the mechanical harvest corn seeds of the high density resistance, thereby being beneficial to improving the selection efficiency.
2. Collecting pollen of drought-resistant corn male parent, applying the pollen to the flowers of drought-resistant corn female parent, and performing once in the morning and afternoon, expanding the population, increasing the density, and in the generation of character separation, especially in the S1 generation with the most obvious separation, increasing the population quantity, generally planting the pollen to 6000 plants/mu or more, wherein the density of the drought-resistant corn male parent and the drought-resistant corn female parent can be properly increased to 7000-8000 plants/mu, and eliminating plants and spike rows with poor agronomic characters such as incoordination between male and female.
Drawings
FIG. 1 is a schematic flow chart of the present invention.
Detailed Description
The following description of the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
The terms first, second and the like in the description and in the claims, are used for distinguishing between similar elements and not necessarily for describing a particular sequential or chronological order. It is to be understood that the data so used may be interchanged, as appropriate, such that embodiments of the present application may be implemented in sequences other than those illustrated or described herein, and that the objects identified by "first," "second," etc. are generally of a type, and are not limited to the number of objects, such as the first object may be one or more. Furthermore, in the description and claims, "and/or" means at least one of the connected objects, and the character "/", generally means that the associated object is an "or" relationship.
In the description of the present invention, it should be understood that the terms "center," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," and the like indicate orientations or positional relationships based on the orientation or positional relationships shown in the drawings, merely to facilitate describing the present invention and simplify the description, and do not indicate or imply that the devices or elements referred to must have a specific orientation, be configured and operated in a specific orientation, and thus should not be construed as limiting the present invention.
In the description of the present invention, it should be noted that, unless explicitly stated and limited otherwise, the terms "mounted," "connected," and "disposed" are to be construed broadly, and may be fixedly connected, disposed, or detachably connected, disposed, or integrally connected, disposed, for example. The specific meaning of the above terms in the present invention will be understood in specific cases by those of ordinary skill in the art.
As shown in FIG. 1, the method for breeding the secret-resistant mechanical-harvest corn seeds provided by the invention comprises the following steps:
firstly, planting ditches, piling up soil, ridging, alternately sowing female parent and male parent on the spaced ridges, wherein the planting density is 5000-6000 plants/mu; the female parent is drought-resistant corn seeds, and the male parent is drought-resistant corn seeds;
According to the flowering-period planting time of the drought-resistant corn female parent and the drought-resistant corn male parent, the full bloom period of the drought-resistant corn female parent is 1-3 days earlier than the pollen scattering period of the drought-resistant corn male parent, corn is not required to pass through different growth and development stages from sowing to maturing, the requirements of the corn on illumination, temperature, precipitation and the like are different in the different growth and development stages, the proper planting climate conditions are required to be known in the corn growth and development, the normal growth and development of the corn is ensured, and the high-quality and high-yield formation of the corn is promoted;
Step two, in the period from seed selection and sowing of corn seeds to seedling emergence, the lowest sprouting temperature of the seeds is about 7.5 ℃, the average temperature is 25-35 ℃, the seedling emergence speed is accelerated along with the rising of the temperature in the temperature range, the average temperature in the corn seedling stage is most suitable when the average temperature is 18.5-19.5 ℃, the proper growth temperature of corn root systems is obtained when the ground temperature at 5cm is 20-25 ℃, and the seedling stage is controlled to normally grow in the weather of more than 20 ℃;
The average air temperature is about 5.6 ℃, the initial day of which the daily average air temperature is more than or equal to 10 ℃ is basically in the middle 5 months of the year, the final day is in the late 10 months, the frost free period is about 145d, the active accumulated temperature of which the temperature is more than or equal to 10 ℃ in the growing season (5-9 months) of corn crops is about 4 ℃ d, and the day-night temperature difference is more suitable, so that the corn planting and seed selection are more suitable;
step three, in the late emergence period of the corn, if the daily sunshine hours exceeds 8 hours, the time of the growth and development period of the corn is advanced; if the number of sunshine hours after emergence is less than 8 hours, the growth period time is shortened;
And fourthly, the sunshine duration in the seed selection and growth season of the corn seeds in the seed selection period of 5-9 months is about 1159 hours, the sunshine duration in6 months is longer, the method is favorable for the formation of seedlings, the illumination condition in the later stage is stronger, the normal operation and accumulation of nutrients in the later stage of grouting and maturation period of the corn can be promoted, the thousand seed weight can be increased, and the corn yield can be improved.
Step five, removing tassel of a drought-resistant corn seed female parent before the pollination period;
step six, obtaining corn of which drought-resistant corn seed female parent plants are cultured, and obtaining corn particle seeds.
According to the quantity ratio of the drought-resistant corn female parent to the drought-resistant corn male parent in the first step of 1-3.5:1, 2 ridges of drought-resistant corn male parent are planted every 2-7 ridges of drought-resistant corn female parent.
According to the number ratio of the drought-resistant corn female parent to the drought-resistant corn male parent in the second step of 4:2, namely planting interval 2-ridge drought-resistant corn male parent between every two 4-ridge drought-resistant corn female parent.
And step four, the method of combining the conventional breeding technology, the haploid breeding technology and the molecular marker assisted selection is utilized to select and hybridize the breakthrough drought-resistant corn female parent and the drought-resistant corn male parent, and in the selection and breeding of the mechanical harvest corn seeds, a proper selection and breeding strategy is adopted, so that the improvement of the selection and breeding efficiency is facilitated.
The artificial supplementary pollination is carried out, the pollen of the male parent of the drought-resistant corn is collected and fed onto the flower filaments of the female parent of the drought-resistant corn, the plant population is enlarged, the density is improved, the plant population is increased in the generation of character separation, particularly in the generation S1 with the most obvious separation, the number of the plant population is increased, the plant population is generally planted to be more than 6000 per mu, the density of the male parent of the drought-resistant corn and the plant parent of the drought-resistant corn can be properly increased to 7000-8000 plants per mu, and plants and spike rows with poor agronomic characters such as incoordination between male and female are eliminated.
In early generation of drought-resistant corn female parent and drought-resistant corn male parent breeding, as far as possible, in different ecological mountain areas, such as islands, additional generation isolation culture breeding is performed, plants with larger ears, better resistance and strong green holding property are selected according to breeding targets, mechanical harvest quality screening is focused, pest and disease resistance screening is performed, and excellent spike rows with higher biological yield are selected.
Preferably, fertilizer is applied to the ridges during planting, wherein the fertilizer comprises 15-20 kg/mu of phosphate fertilizer and 5-10 kg/mu of urea;
in the heading stage, 50% of nitrogen-phosphorus-potassium compound fertilizer 15 kg/mu and urea 15 kg/mu are applied;
Watering for 4-6 times in the whole growth and development period.
The embodiments of the present application have been described above with reference to the accompanying drawings, but the present application is not limited to the above-described embodiments, which are merely illustrative and not restrictive, and many forms may be made by those having ordinary skill in the art without departing from the spirit of the present application and the scope of the claims, which are to be protected by the present application.
Claims (7)
1. A method for breeding a secret-resistant mechanical-harvest corn seed is characterized by comprising the following steps:
Firstly, planting ditches, piling up soil, ridging, alternately sowing female parent and male parent on the spaced ridges, wherein the planting density is 5000-6000 plants/mu; the female parent is drought-resistant corn seeds, and the male parent is drought-resistant corn seeds;
According to the flowering-period planting time of the drought-resistant corn female parent and the drought-resistant corn male parent, the full bloom period of the drought-resistant corn female parent is 1-3 days earlier than the pollen scattering period of the drought-resistant corn male parent, corn is not required to pass through different growth and development stages from sowing to maturing, the requirements of the corn on illumination, temperature, precipitation and the like are different in the different growth and development stages, the proper planting climate conditions are required to be known in the corn growth and development, the normal growth and development of the corn is ensured, and the high-quality and high-yield formation of the corn is promoted;
Step two, in the period from seed selection and sowing of corn seeds to seedling emergence, the lowest sprouting temperature of the seeds is about 7.5 ℃, the average temperature is 25-35 ℃, the seedling emergence speed is accelerated along with the rising of the temperature in the temperature range, the average temperature in the corn seedling stage is most suitable when the average temperature is 18.5-19.5 ℃, the proper growth temperature of corn root systems is obtained when the ground temperature at 5cm is 20-25 ℃, and the seedling stage is controlled to normally grow in the weather of more than 20 ℃;
The average air temperature is about 5.6 ℃, the initial day of which the daily average air temperature is more than or equal to 10 ℃ is basically in the middle 5 months of the year, the final day is in the late 10 months, the frost free period is about 145d, the active accumulated temperature of which the temperature is more than or equal to 10 ℃ in the growing season (5-9 months) of corn crops is about 4 ℃ d, and the day-night temperature difference is more suitable, so that the corn planting and seed selection are more suitable;
step three, in the late emergence period of the corn, if the daily sunshine hours exceeds 8 hours, the time of the growth and development period of the corn is advanced; if the number of sunshine hours after emergence is less than 8 hours, the growth period time is shortened;
And fourthly, the sunshine duration in the seed selection and growth season of the corn seeds in the seed selection period of 5-9 months is about 1159 hours, the sunshine duration in6 months is longer, the method is favorable for the formation of seedlings, the illumination condition in the later stage is stronger, the normal operation and accumulation of nutrients in the later stage of grouting and maturation period of the corn can be promoted, the thousand seed weight can be increased, and the corn yield can be improved.
Step five, removing tassel of a drought-resistant corn seed female parent before the pollination period;
step six, obtaining corn of which drought-resistant corn seed female parent plants are cultured, and obtaining corn particle seeds.
2. The method for breeding the secret-resistant mechanical-harvest corn seeds according to claim 1, which is characterized by comprising the following steps: in the first step, the number ratio of the drought-resistant corn female parent to the drought-resistant corn male parent is 1-3.5:1, namely 2 ridges of drought-resistant corn male parent are planted in every 2-7 ridges of drought-resistant corn female parent.
3. The method for breeding the secret-resistant mechanical-harvest corn seeds according to claim 1, which is characterized by comprising the following steps: in the second step, the number ratio of the drought-resistant corn female parent to the drought-resistant corn male parent is 4:2, namely, 2-ridge drought-resistant corn male parent is planted in each interval of 4-ridge drought-resistant corn female parent.
4. The method for breeding the secret-resistant mechanical-harvest corn seeds according to claim 3, which is characterized by comprising the following steps: and step four, a method combining a conventional breeding technology, a haploid breeding technology and molecular marker assisted selection is utilized to select and hybridize a breakthrough drought-resistant corn female parent and a drought-resistant corn male parent, and in the selection and breeding of the mechanical harvest corn seeds, a proper selection and breeding strategy is adopted, so that the improvement of the selection and breeding efficiency is facilitated.
5. The method for breeding the secret-resistant mechanical-harvest corn seeds according to claim 3, which is characterized by comprising the following steps: the artificial supplementary pollination is carried out, the pollen of the male parent of the drought-resistant corn is collected and fed onto the flower filaments of the female parent of the drought-resistant corn, the plant population is enlarged, the density is improved, the plant population is increased in the generation of character separation, particularly in the S1 generation with the most obvious separation, the plant population is generally planted to be more than 6000 plants/mu, the density of the male parent of the drought-resistant corn and the plant parent of the drought-resistant corn can be properly increased to 7000-8000 plants/mu, and plants and spike rows with poor agronomic characters such as incoordination between male and female are eliminated.
6. The method for breeding the secret-resistant mechanical-harvest corn seeds according to claim 3, which is characterized by comprising the following steps: in early generation of drought-resistant corn female parent and drought-resistant corn male parent breeding, as far as possible, in different ecological mountain areas, such as islands, additional generation isolation culture breeding is performed, plants with larger ears, better resistance and strong green holding property are selected according to breeding targets, mechanical harvest quality screening is focused, pest and disease resistance screening is performed, and excellent spike rows with higher biological yield are selected.
7. The method for breeding the secret-resistant mechanical-harvest corn seeds according to claim 3, which is characterized by comprising the following steps: when in planting, fertilizer is applied on the ridges, wherein the fertilizer comprises 15-20 kg/mu of phosphate fertilizer and 5-10 kg/mu of urea;
in the heading stage, 50% of nitrogen-phosphorus-potassium compound fertilizer 15 kg/mu and urea 15 kg/mu are applied;
Watering for 4-6 times in the whole growth and development period.
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