CN117898066A - Corn seed vigor germination event determination method - Google Patents
Corn seed vigor germination event determination method Download PDFInfo
- Publication number
- CN117898066A CN117898066A CN202410120684.2A CN202410120684A CN117898066A CN 117898066 A CN117898066 A CN 117898066A CN 202410120684 A CN202410120684 A CN 202410120684A CN 117898066 A CN117898066 A CN 117898066A
- Authority
- CN
- China
- Prior art keywords
- germination
- seeds
- test
- corn
- groups
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 230000035784 germination Effects 0.000 title claims abstract description 65
- 240000008042 Zea mays Species 0.000 title claims abstract description 32
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 title claims abstract description 32
- 235000002017 Zea mays subsp mays Nutrition 0.000 title claims abstract description 32
- 235000005822 corn Nutrition 0.000 title claims abstract description 32
- 238000000034 method Methods 0.000 title claims abstract description 21
- 238000002791 soaking Methods 0.000 claims abstract description 13
- 241000238631 Hexapoda Species 0.000 claims abstract description 4
- 201000010099 disease Diseases 0.000 claims abstract description 4
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims abstract description 4
- 238000005286 illumination Methods 0.000 claims description 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 7
- 229930191978 Gibberellin Natural products 0.000 claims description 4
- IXORZMNAPKEEDV-UHFFFAOYSA-N gibberellic acid GA3 Natural products OC(=O)C1C2(C3)CC(=C)C3(O)CCC2C2(C=CC3O)C1C3(C)C(=O)O2 IXORZMNAPKEEDV-UHFFFAOYSA-N 0.000 claims description 4
- 239000003448 gibberellin Substances 0.000 claims description 4
- 230000012010 growth Effects 0.000 claims description 4
- 230000035899 viability Effects 0.000 claims description 2
- 230000009286 beneficial effect Effects 0.000 abstract description 2
- 230000004083 survival effect Effects 0.000 abstract description 2
- 238000005259 measurement Methods 0.000 description 8
- 230000000694 effects Effects 0.000 description 6
- 241000196324 Embryophyta Species 0.000 description 4
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 2
- 238000012271 agricultural production Methods 0.000 description 2
- 229910052700 potassium Inorganic materials 0.000 description 2
- 239000011591 potassium Substances 0.000 description 2
- 229920002472 Starch Polymers 0.000 description 1
- 150000001768 cations Chemical class 0.000 description 1
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000008635 plant growth Effects 0.000 description 1
- 230000014284 seed dormancy process Effects 0.000 description 1
- 230000007226 seed germination Effects 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/02—Germinating apparatus; Determining germination capacity of seeds or the like
- A01C1/025—Testing seeds for determining their viability or germination capacity
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Physiology (AREA)
- Soil Sciences (AREA)
- Environmental Sciences (AREA)
- Pretreatment Of Seeds And Plants (AREA)
Abstract
The application discloses a corn seed vitality germination event determination method, which comprises the following steps: s1, seed treatment, namely selecting corn which is free of damage of diseases and insects and full and unbroken as seeds, and soaking the seeds; s2, carrying out a grouping test, namely respectively carrying out a standard germination test, a low-temperature germination test, a high Wen Faya test and a field germination test on the corn seeds treated in the step S1; s3, measuring the vitality of seedlings of each group of test seeds. The application has the beneficial effects that the corn seeds are respectively subjected to standard germination test, low-temperature germination test, high Wen Faya test and field germination test, and the vitality values of the corn seeds under different conditions are measured, so that the germination rate, the low-temperature and high-temperature resistance and the natural survival ability of the corn seeds under natural conditions are judged.
Description
Technical Field
The invention relates to the technical field of corn seed vitality determination, in particular to a corn seed vitality germination event determination method.
Background
Seeds are the most basic production data of agriculture, are the basis of agricultural production, and improvement of seed quality is an important measure for guaranteeing high efficiency, high quality and high yield of agricultural production. The seed vigor refers to the potential capability of determining the rapid and orderly emergence of seeds and the growth of normal seedlings, and is an important index for measuring the quality of the seeds.
When the vitality of the seeds is lower, the problems of seedling lack and poor plant growth condition can be caused, so that the planting yield is influenced, economic loss is caused, the germination of the seeds is influenced by various conditions, and under different conditions, the vitality values of the seeds can be greatly different, so that the vitality values of the seeds under different influence events need to be measured, and the optimal germination conditions of the seeds are obtained.
Disclosure of Invention
In order to solve the problems, the invention provides a method for measuring the vigor germination event of corn seeds, which is realized by the following technical scheme.
A method for determining a germination event of corn seeds, the method comprising the steps of:
S1, seed treatment, namely selecting corn which is free of damage of diseases and insects and full and unbroken as seeds, and soaking the seeds;
S2, carrying out a grouping test, namely respectively carrying out a standard germination test, a low-temperature germination test, a high Wen Faya test and a field germination test on the corn seeds treated in the step S1;
s3, measuring the vitality of seedlings of each group of test seeds.
Preferably, in the step S1, the temperature of the soaking water is 20±1 ℃ and the soaking time is 12 hours.
Preferably, 0.2% KCl and 150mg/L gibberellin are added to the water during soaking.
Preferably, in the step S2, 200 seeds are selected and divided into two groups for germination test, and the two groups of seeds are subjected to germination culture in an illumination incubator at 25 ℃ for 8 days, and then subjected to activity measurement.
Preferably, in the step S2, 200 seeds are selected and divided into two groups during the low-temperature germination test, the two groups of seeds are treated for 7 days under the dark condition of 8 ℃, then the germination culture is carried out in an illumination incubator of 25 ℃, and the activity measurement is carried out after 8 days.
Preferably, in the step S2, 200 seeds are selected and divided into two groups during the high-temperature germination test, the two groups of seeds are treated for 1 day at 40 ℃, then germination culture is performed in an illumination incubator at 25 ℃, and activity measurement is performed after 8 days.
Preferably, in the step S2, 200 seeds are selected and divided into two groups during the field germination test, the two groups of seeds are sown in the field, and the vitality is measured after 8 days.
Preferably, the method for calculating the vitality measurement comprises the following steps: germination rate x seedling weight.
Preferably, in the grouping test, 10 seedlings with the best growth condition are selected for each group, the total weight of 10 seedlings is measured, and the total weight of 10 seedlings is divided by 10 as the weight of seedlings in the group.
The application has the beneficial effects that the corn seeds are respectively subjected to standard germination test, low-temperature germination test, high Wen Faya test and field germination test, and the vitality values of the corn seeds under different conditions are measured, so that the germination rate, the low-temperature and high-temperature resistance and the natural survival ability of the corn seeds under natural conditions are judged.
Detailed Description
The following description of the embodiments of the present invention will be made clearly and completely, and it is apparent that the described embodiments are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
A method for determining a germination event of corn seeds, the method comprising the steps of:
S1, seed treatment, namely selecting corn which is free of damage of diseases and insects and full and unbroken as seeds, and soaking the seeds;
S2, carrying out a grouping test, namely respectively carrying out a standard germination test, a low-temperature germination test, a high Wen Faya test and a field germination test on the corn seeds treated in the step S1;
s3, measuring the vitality of seedlings of each group of test seeds.
Further, in step S1, the temperature of the soaking water is 20+/-1 ℃ and the soaking time is 12 hours.
The corn seeds can be fully soaked by soaking, so that the seed coats can be expanded and softened, and radicle and hypocotyl are enabled to be active due to water, so that germination is easier.
Further, 0.2% KCl and 150mg/L gibberellin are added to the water during soaking.
Potassium is a monovalent cation necessary for plant organism, and is dissolved in plant juice in an ionic state, and the main functions of potassium are related to plant metabolism, so that the germination rate, germination vigor and fresh and alive quality of corn seeds can be rapidly and effectively improved; gibberellin is used as a plant regulator, and can break seed dormancy, accelerate starch hydrolysis in endosperm and promote seed germination.
Further, in step S2, 200 seeds are selected and divided into two groups for germination test, and the two groups of seeds are subjected to germination culture in an illumination incubator at 25 ℃ for 8 days, and then subjected to activity measurement.
The viability of the corn seeds under standard conditions was judged by a standard germination test.
Further, in step S2, 200 seeds are selected and divided into two groups during the low-temperature germination test, the two groups of seeds are treated for 7 days under the dark condition of 8 ℃, then the germination culture is carried out in an illumination incubator of 25 ℃, and the activity measurement is carried out after 8 days.
The low temperature resistance of the seeds was measured by a low temperature germination test.
Further, in step S2, 200 seeds are selected and divided into two groups during the high-temperature germination test, the two groups of seeds are treated for 1 day at 40 ℃, then germination culture is performed in an illumination incubator at 25 ℃, and activity measurement is performed after 8 days.
The high temperature resistance of the seeds was measured by the high Wen Faya test.
Further, in step S2, 200 seeds are selected and divided into two groups during field germination test, and the two groups of seeds are sowed in the field for 8 days, and then the vitality is measured.
And measuring the vitality of the seeds under the natural planting condition through a field germination test.
Further, the method for calculating the vitality measurement comprises the following steps: germination rate x seedling weight.
Further, in the grouping test, 10 seedlings with the best growth condition were selected for each group, the total weight of 10 seedlings was measured, and the total weight of 10 seedlings was divided by 10 as the seedling weight of the group.
The preferred embodiments of the invention disclosed above are intended only to assist in the explanation of the invention. The preferred embodiments are not intended to be exhaustive or to limit the invention to the precise form disclosed. Obviously, many modifications and variations are possible in light of the above teaching. The embodiments were chosen and described in order to best explain the principles of the invention and the practical application, to thereby enable others skilled in the art to best understand and utilize the invention. The invention is limited only by the claims and the full scope and equivalents thereof.
Claims (9)
1. A method for determining a germination event of corn seeds, comprising the steps of:
S1, seed treatment, namely selecting corn which is free of damage of diseases and insects and full and unbroken as seeds, and soaking the seeds;
S2, carrying out a grouping test, namely respectively carrying out a standard germination test, a low-temperature germination test, a high Wen Faya test and a field germination test on the corn seeds treated in the step S1;
s3, measuring the vitality of seedlings of each group of test seeds.
2. The method according to claim 1, wherein in the step S1, the soaking water is at 20±1 ℃ for 12 hours.
3. The method for determining germination event of corn seeds according to claim 2, wherein 0.2% KCl and 150mg/L gibberellin are added to the water during soaking.
4. The method according to claim 1, wherein in the step S2, 200 seeds are selected for germination test and two groups of seeds are germinated and cultured in a light incubator at 25 ℃ for 8 days, and the viability is measured.
5. The method for determining germination event of corn seeds according to claim 1, wherein in the step S2, 200 seeds are selected and divided into two groups, and the two groups of seeds are subjected to germination cultivation in a light incubator at 25 ℃ after being treated for 7 days in a dark condition at 8 ℃ and subjected to germination determination after 8 days.
6. The method for determining germination event of corn seeds according to claim 1, wherein in the step S2, 200 seeds are selected and divided into two groups, and the two groups of seeds are subjected to germination cultivation in an illumination incubator at 40 ℃ for 1 day and then subjected to germination cultivation for 8 days.
7. The method for determining vigor germination event of corn seeds according to claim 1, wherein in the step S2, 200 seeds are selected and divided into two groups for field germination test, and the two groups are sown in the field 8 days later for vigor determination.
8. A method for determining a vigour germination event for corn seeds according to any one of claims 4 to 7, wherein the method for calculating the vigour determination is as follows: germination rate x seedling weight.
9. A method for determining germination events of corn seeds according to claim 8, characterized in that in the group test, 10 seedlings with the best growth are selected for each group, the total weight of 10 seedlings is measured, and the total weight of 10 seedlings is divided by 10 as the weight of seedlings in the group.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202410120684.2A CN117898066A (en) | 2024-01-29 | 2024-01-29 | Corn seed vigor germination event determination method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202410120684.2A CN117898066A (en) | 2024-01-29 | 2024-01-29 | Corn seed vigor germination event determination method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN117898066A true CN117898066A (en) | 2024-04-19 |
Family
ID=90690647
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202410120684.2A Pending CN117898066A (en) | 2024-01-29 | 2024-01-29 | Corn seed vigor germination event determination method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN117898066A (en) |
-
2024
- 2024-01-29 CN CN202410120684.2A patent/CN117898066A/en active Pending
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104335734B (en) | The method that the injury of Semen Maydis difference environment stress is alleviated in brassinosteroid seed soaking | |
| CN107135782B (en) | Cultivation method of southern Xinjiang membraneless cotton | |
| CN104920089B (en) | A kind of resistance to screening technique for flooding parent of Direct-seeding Rice breeding | |
| CN112262637A (en) | Salt tolerance identification and evaluation method for hybrid rice variety | |
| CN111480563A (en) | Water culture method for experimental rice cultivated in greenhouse | |
| CN109463221B (en) | Method for screening drought-tolerant cabbage type rape variety | |
| CN112020930B (en) | Method for inducing rice to improve salt tolerance | |
| JP7454587B2 (en) | Plant growth regulators and their applications | |
| CN114885633B (en) | Method for promoting germination of peanut seeds under low-temperature stress | |
| CN111011208A (en) | Method for screening salt-tolerant cotton varieties | |
| CN110771497A (en) | Quinoa saline-alkali-resistant lodging-resistant breeding method | |
| CN117898066A (en) | Corn seed vigor germination event determination method | |
| CN112544371B (en) | Method for identifying low temperature resistance of maize inbred line in germination period | |
| CN105993924B (en) | A kind of application of the selection of the high yield of resistance to dense planting Brassica napus hybrid kind and obtained napus hybrid kind | |
| CN113016518B (en) | Resistance identification method for celery leaf spot | |
| CN109511407B (en) | Method for rapidly identifying drought tolerance of radish | |
| CN108012618A (en) | A kind of rice intelligence presoaking and germinating system and germination accelerating method | |
| CN107980614B (en) | Hybrid wheat florescence adjusting seed production method | |
| Eskandari et al. | Effect of planting pattern and irrigation system on germination performance of maize seeds harvested at different times of maturation | |
| CN112760397A (en) | Application of Talaromyces wortmannii in crop drought resistance and growth promotion | |
| CN105557118B (en) | A kind of method for treating seeds improving emergence rate under ridged pot without plow low temperature stress | |
| CN110476995A (en) | A kind of cotton coating type root-growing agent and its preparation method and application | |
| CN110089251A (en) | A kind of fertilizing method improving melon fruit nutritional quality and sugar content | |
| CN109463063A (en) | Machine transplanting of rice late rice seed sigh effect azoles solution seed soaking breeding method | |
| CN117581721B (en) | Method for improving salt tolerance of rice in seedling stage by using triptfordine |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication |