CN117866753A - Plant tissue culture control system, method and plant tissue culture device - Google Patents

Abstract

The invention provides a plant tissue culture control system, a plant tissue culture method and a plant tissue culture device, wherein the plant tissue culture system comprises a control center module, a reactor control unit and a liquid exchange device which are sequentially connected; the liquid exchange device is connected with a plurality of bioreactors arranged outside; the control center module is used for acquiring the culture parameters of the current culture batch and sending the culture parameters to the reactor control unit; and the reactor control unit is used for determining a target bioreactor associated with the current culture batch from the plurality of bioreactors, controlling the liquid changing device to clean the target bioreactors so as to discharge waste liquid, discharging a new culture medium corresponding to the current culture batch into the target bioreactors, controlling the target bioreactors to culture according to culture parameters, and controlling the liquid changing device to discharge the culture medium in the target bioreactors after the culture is finished. According to the invention, through an automatic control system, the efficiency and consistency of plant tissue culture can be improved, and batch production can be realized.

Description

Plant tissue culture control system, method and plant tissue culture device

Technical Field

The invention relates to the technical field of automatic control of fermentation culture, in particular to a plant tissue culture control system, a plant tissue culture method and a plant tissue culture device.

Background

In the plant tissue culture process, the culture solution and the gas need to be replaced periodically. Currently, this process relies mainly on manual operations, especially in mass production and experiments, requiring large numbers of reactors for plant tissue culture. The liquid and air exchange of the reactor are carried out by manual operation, so that the efficiency is low, and the consistency is difficult to ensure; in mass production, manual operation cannot realize operations such as liquid filling, accurate recording of soaking time, liquid discharging and the like at the same time. The existing culture quantity and the requirement on environmental sterility are gradually increased, and how to realize the batch and large-scale production of the reactor, ensure the sterility of the production environment and improve the production quality is a problem to be solved in the prior art.

Disclosure of Invention

In view of the above, the invention aims to provide a plant tissue culture control system, a plant tissue culture control method and a plant tissue culture device, which can reduce the dependence on manual operation in the plant tissue culture process, improve the efficiency and consistency of plant tissue culture, realize batch and large-scale production of a reactor and further ensure the sterility of a production environment through an automatic control system.

In a first aspect, an embodiment of the present invention provides a plant tissue culture control system, including a control center module, a reactor control unit, and a liquid exchange device, which are sequentially connected; the liquid exchange device is connected with a plurality of bioreactors arranged outside; the control center module is used for acquiring the culture parameters of the current culture batch and sending the culture parameters to the reactor control unit; the culture parameters comprise culture time, soaking interval and soaking time; and the reactor control unit is used for determining a target bioreactor associated with the current culture batch from the plurality of bioreactors, controlling the liquid changing device to clean the target bioreactors so as to discharge waste liquid, discharging a new culture medium corresponding to the current culture batch into the target bioreactors, controlling the target bioreactors to culture according to culture parameters, and controlling the liquid changing device to discharge the culture medium in the target bioreactors after the culture is finished.

In a preferred embodiment of the invention, the liquid exchanging device comprises a liquid inlet valve, a liquid outlet valve and a liquid exchanging power unit; the liquid inlet valve and the liquid outlet valve are both arranged on the bioreactor; the hydrodynamic force unit is used for providing hydrodynamic force to discharge the culture medium into or out of the bioreactor.

In a preferred embodiment of the invention, the hydraulic power exchanging unit comprises an air pump, an air bottle and a connecting pipeline; the air pump is connected with the air bottle, and is also connected with the liquid inlet valve and the liquid outlet valve through connecting pipelines respectively; the air pump is used to discharge the culture medium into or out of the bioreactor by air pressure.

In a preferred embodiment of the invention, the liquid exchange device further comprises a waste liquid treatment valve; the waste liquid treatment valve is arranged on the bioreactor; the waste liquid treatment valve is connected with the reactor control unit; the waste liquid treatment valve is used for receiving an opening instruction of the reactor control unit and opening the valve to discharge waste liquid in the bioreactor; and the device is also used for receiving a closing instruction of the reactor control unit and closing the valve to prevent the waste liquid from flowing back to the bioreactor.

In a preferred embodiment of the invention, the system further comprises a ventilation device; the air interchanger is connected with the control center module; the ventilation device is used for receiving the control instruction sent by the control center module, and exchanging air outside the target bioreactor according to the control instruction so as to ensure the sterility of the environment outside the target bioreactor in the cultivation process.

In a preferred embodiment of the invention, the ventilation device comprises a filter fan unit; the filtering fan unit is used for filtering particles and microorganisms in the air so as to send the filtered air into the environment outside the bioreactor for gas exchange.

In a preferred embodiment of the invention, the control system further comprises a precise soaking regulation and control module, and the precise soaking regulation and control module is connected with the control center module; the control center module is also used for calculating the soaking preparation time of the current culture batch; the soaking preparation time period comprises a flushing pipe time period for cleaning by using a new culture medium and a flowing time period for the new culture medium to flow to the target bioreactor; the accurate soaking regulation and control module is used for controlling the bioreactor to execute a preparation process before soaking starts before reaching a soaking time point based on the soaking preparation time; and receiving a control instruction of the control center module, and automatically controlling liquid inlet and liquid outlet of the bioreactor to realize automatic filling and discharging of the culture medium.

In a preferred embodiment of the invention, the control system further comprises an alarm intervention module, and the alarm intervention module is connected with the control center module; the control center module is also used for monitoring the running state of each device in the plant tissue culture control system, and sending a warning instruction to the warning intervention module when the abnormal running of the device is monitored; the alarm intervention module is used for sending out an alarm prompt when receiving an alarm instruction so as to prompt personnel to perform human intervention treatment.

In a preferred embodiment of the present invention, the control center module includes a man-machine interaction unit and a display unit; the human-computer interaction unit is used for providing a human-computer interaction interface and receiving a user input instruction; the display unit is used for displaying the running state of the control system and the liquid change information.

In the preferred embodiment of the invention, the control center module is an upper computer, and the reactor control unit is a PLC programmable logic controller.

In a second aspect, an embodiment of the present invention further provides a plant tissue culture apparatus, including a plant tissue culture control system according to any one of the embodiments of the first aspect, and further including a plurality of bioreactors; each bioreactor is connected with a liquid exchange device in the plant tissue culture control system.

In a third aspect, an embodiment of the present invention further provides a plant tissue culture control method, which is applied to the plant tissue culture control system in any one of the embodiments of the first aspect; the method comprises the following steps: obtaining culture parameters of a current culture batch; the culture parameters comprise culture time, soaking interval and soaking time; determining a target bioreactor associated with the current culture batch; the target bioreactor is cleaned by a liquid exchanging device to discharge waste liquid; discharging a new culture medium corresponding to the current culture batch into the target bioreactor through the liquid exchange device; controlling the target bioreactor to culture according to the culture parameters; after the completion of the culture, the medium in the target bioreactor is discharged by the liquid exchange device.

The embodiment of the invention has the following beneficial effects:

the embodiment of the invention provides a plant tissue culture control system, a plant tissue culture method and a plant tissue culture device, wherein the system comprises a control center module, a reactor control unit and a liquid exchange device which are sequentially connected; the liquid exchange device is connected with a plurality of bioreactors arranged outside; the control center module is used for acquiring the culture parameters of the current culture batch and sending the culture parameters to the reactor control unit; the culture parameters comprise culture time, soaking interval and soaking time; and the reactor control unit is used for determining a target bioreactor associated with the current culture batch from the plurality of bioreactors, controlling the liquid changing device to clean the target bioreactors so as to discharge waste liquid, discharging a new culture medium corresponding to the current culture batch into the target bioreactors, controlling the target bioreactors to culture according to culture parameters, and controlling the liquid changing device to discharge the culture medium in the target bioreactors after the culture is finished. In the system, the control center module and the reactor control unit are introduced to control the liquid exchange device to realize automatic treatment of waste liquid cleaning, liquid inlet, liquid outlet and the like of the bioreactor, realize full-automatic control of the culture process, reduce the dependence on manual operation in the plant tissue culture process, improve the efficiency and consistency of plant tissue culture, realize batch and large-scale production of the reactor and further ensure the sterility of the production environment.

Additional features and advantages of the disclosure will be set forth in the description which follows, or in part will be obvious from the description, or may be learned by practice of the techniques of the disclosure.

The foregoing objects, features and advantages of the disclosure will be more readily apparent from the following detailed description of the preferred embodiments taken in conjunction with the accompanying drawings.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings that are needed in the description of the embodiments or the prior art will be briefly described, and it is obvious that the drawings in the description below are some embodiments of the present invention, and other drawings can be obtained according to the drawings without inventive effort for a person skilled in the art.

FIG. 1 is a schematic diagram of a plant tissue culture control system according to an embodiment of the present invention;

fig. 2 is a schematic structural diagram of a liquid exchange device according to an embodiment of the present invention;

FIG. 3 is a schematic structural diagram of another liquid exchange device according to an embodiment of the present invention;

FIG. 4 is a schematic diagram of another plant tissue culture control system according to an embodiment of the present invention;

FIG. 5 is a schematic diagram of another plant tissue culture control system according to an embodiment of the present invention;

FIG. 6 is a schematic diagram of another plant tissue culture control system according to an embodiment of the present invention;

FIG. 7 is a schematic diagram of a plant tissue culture apparatus according to an embodiment of the present invention;

FIG. 8 is a schematic structural diagram of a plant tissue culture device based on reaction chamber control according to an embodiment of the present invention;

FIG. 9 is a schematic diagram of a bioreactor arrangement in a reaction chamber according to an embodiment of the present invention;

FIG. 10 is a schematic view of a pipeline structure inside a reaction chamber according to an embodiment of the present invention;

FIG. 11 is a schematic diagram illustrating connection between a connecting pipeline and a connector terminal according to an embodiment of the present invention;

FIG. 12 is a schematic view of a bioreactor according to an embodiment of the present invention;

fig. 13 is a schematic flow chart of a plant tissue culture control method according to an embodiment of the present invention.

Icon: 1-a bioreactor; 2-reaction chamber; 3-turning up the door; 4-a control screen; 5-a filter fan unit; 6-an air pump; 7-layer frames; 8-connecting pipelines; 9-tab terminals.

Detailed Description

For the purpose of making the objects, technical solutions and advantages of the embodiments of the present invention more apparent, the technical solutions of the present invention will be clearly and completely described below with reference to the accompanying drawings, and it is apparent that the described embodiments are some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.

Considering that the existing culture amount and the requirement on environmental sterility are gradually increased, the current plant tissue culture process needs to rely on manual operation to change liquid and air of the reactor, has low efficiency, is difficult to ensure consistency, and cannot realize batch large-scale production of the reactor.

For the convenience of understanding the present embodiment, a plant tissue culture control system disclosed in the embodiment of the present invention will be described in detail.

Embodiment one:

referring to fig. 1, a schematic structural diagram of a plant tissue culture control system is shown, and as can be seen from fig. 1, the system includes a control center module, a reactor control unit and a liquid exchange device, which are sequentially connected. Wherein, the liquid changing device is connected with a plurality of bioreactors arranged outside.

In the system, the control center module is used for acquiring the culture parameters of the current culture batch and sending the culture parameters to the reactor control unit. The control center module is a core part of the system and is responsible for acquiring and processing culture parameters. For example, the control center module may include a central processor and a memory for storing and processing the incubation parameters. In one embodiment, the control center module may be a host computer. Further, the control center module comprises a human-computer interaction unit and a display unit, wherein the human-computer interaction unit is a unit for providing a human-computer interaction interface and receiving a user input instruction. For example, the human-computer interaction unit may include an input device such as a touch screen, a keyboard, a mouse, etc., through which a user can input instructions, such as setting incubation parameters, starting an incubation process, stopping an incubation process, etc. In addition, the man-machine interaction unit can also comprise a voice recognition module, so that the system has a voice interaction function, and the user experience is further improved. Further, the display unit means a unit for displaying the operation state of the control system and the liquid change information. For example, the display unit may be a liquid crystal display screen, an LED display screen, etc., and the operation state of the control system includes the operation or idle state of the reactor, displays the cultivation progress of the current cultivation batch, and displays the cultivation time; the liquid changing information comprises the number of times of liquid changing and the prompt of the next liquid changing time, liquid changing and soaking information; the method can also be used for displaying key data in the culture process, such as parameters of culture time, temperature, humidity, illumination and the like, and information of the state, concentration and the like of the culture solution in real time.

In this embodiment, the above-mentioned culture parameters include a culture time, a soaking interval, and a soaking time. For example, the cultivation time may be several days to several weeks, depending on the type of plant tissue cultivated and the growth rate. The soaking interval and soaking time may be once per day or once per week depending on the culture requirements. In actual operation, the culture parameters can be dynamically adjusted according to the growth conditions of the culture monitored in real time so as to optimize the culture effect.

In the system, the reactor control unit is used for determining a target bioreactor associated with a current culture batch from a plurality of bioreactors, controlling the liquid changing device to clean the target bioreactor to discharge waste liquid, discharging new culture medium corresponding to the current culture batch into the target bioreactor, controlling the target bioreactor to culture according to culture parameters, and controlling the liquid changing device to discharge the culture medium in the target bioreactor after the culture is finished. Wherein, for a determined target culture or culture batch, the system can automatically complete the liquid-changing soaking culture cycle for a plurality of times by a reactor control unit based on culture parameters and the like in the process from the beginning to the end of the culture until the target culture or culture batch is completed. For example, in a culture batch of 30 weeks, the liquid change soaking process may be performed once for 6 hours or once for 24 hours, and the specific number of times may be performed according to the culture parameters. In addition, the amount of the liquid change may be controlled, for example, when a new medium is discharged into the target bioreactor, the amount of the liquid change may be controlled to be the same as the amount of the liquid change, or may be controlled to be 2/3 of the amount of the liquid change, and the specific amount may be controlled according to the culture parameters.

Here, the bioreactor is a vessel for culture cultivation. The bioreactor forms a sealing state with the liquid changing device after inoculating or implanting the culture, ensures the smooth liquid changing process and the sterility of the culture process. In practice, the bioreactor may be a glass or plastic bottle, with the plant tissue to be cultivated inside. The bioreactor can be made of different materials and structures to adapt to different types of plant tissue culture requirements. Before the culture is cultivated, inoculation treatment is required, which is to sterilize a reactor, a culture medium, a matched pipeline and the like. If seed cells prepared for inoculation in advance are mixed with the culture medium, they are directly poured into the sterilized reactor (which can be operated in a sterile clean bench). And then automatically completing the culture process according to the culture flow under the automatic operation of the control system.

The reactor control unit may include a sensor for detecting the liquid level in the bioreactor to determine whether a cleaning or draining operation is required. In one embodiment, the reactor control unit is a PLC programmable logic controller.

In this system, the liquid change device is used for both cleaning and liquid feed and discharge operations. For example, the fluid changing device may comprise a pump and a valve for controlling the flow of the fluid. Fig. 2 is a schematic structural diagram of a liquid exchange device, where the liquid exchange device includes a liquid inlet valve, a liquid outlet valve, and a liquid exchange power unit. The inlet valve and the outlet valve are both arranged on the bioreactor, wherein the inlet valve is used for controlling the inflow of the culture medium, which may be of any suitable valve type, such as a ball valve, a shut-off valve or a butterfly valve. The outlet valve is used to control the outflow of the medium and may be of the same or a different valve type than the inlet valve. The liquid outlet valve is used for controlling the flow and pressure of the culture medium so as to discharge the waste liquid in the bioreactor or sample.

The liquid exchanging power unit is used for providing liquid exchanging power to discharge the culture medium into or out of the bioreactor. The fluid-exchange power unit may be a motor, pump or other power device for driving the flow of the culture medium. In one embodiment, the hydraulic power exchanging unit comprises an air pump, an air bottle and a connecting pipeline; the air pump is connected with the air bottle, and is also connected with the liquid inlet valve and the liquid outlet valve through connecting pipelines respectively; the air pump is used to discharge the culture medium into or out of the bioreactor by air pressure.

In addition, the gas in the gas cylinder and the gas pump and gas pump lines may be sterilized in order to ensure a sterile environment in the reactor. Furthermore, for different cultures, the gas in the gas cylinder may be replaced by a gas in the gas cylinder, such as oxygen, nitrogen, carbon dioxide, etc., or a mixture of these gases, based on the requirements of the culture for the type of gas and the concentration of the gas.

In actual operation, in order to improve the convenience of waste liquid recovery, this embodiment further provides another implementation manner of the liquid exchange device, as shown in fig. 3, which is a schematic structural diagram of another liquid exchange device, and on the basis of fig. 2, the liquid exchange device further includes a waste liquid treatment valve, where the waste liquid treatment valve is disposed on the bioreactor, and the waste liquid treatment valve is connected to the reactor control unit. The waste liquid treatment valve is used for receiving an opening instruction of the reactor control unit and opening the valve to discharge waste liquid in the bioreactor; and the device is also used for receiving a closing instruction of the reactor control unit and closing the valve to prevent the waste liquid from flowing back to the bioreactor.

Compared with the existing culture technology, in the prior art, a single reactor or a plurality of reactors are generally used for liquid exchange and air exchange through manual operation in plant tissue culture, and the dependency on manual work is serious. And the plant tissue culture control system provided by the application is used for controlling the liquid changing device to realize automatic treatment of waste liquid cleaning, liquid inlet, liquid outlet and the like of the bioreactor through introducing the control center module and the reactor control unit, realizing full-automatic control of the culture process, reducing the dependence on manual operation in the plant tissue culture process, improving the efficiency and consistency of plant tissue culture, realizing batch large-scale production of the reactor and further ensuring the sterility of the production environment.

The embodiment of the invention provides a plant tissue culture control system, which comprises a control center module, a reactor control unit and a liquid exchange device which are sequentially connected; the liquid exchange device is connected with a plurality of bioreactors arranged outside; the control center module is used for acquiring the culture parameters of the current culture batch and sending the culture parameters to the reactor control unit; the culture parameters comprise culture time, soaking interval and soaking time; and the reactor control unit is used for determining a target bioreactor associated with the current culture batch from the plurality of bioreactors, controlling the liquid changing device to clean the target bioreactors so as to discharge waste liquid, discharging a new culture medium corresponding to the current culture batch into the target bioreactors, controlling the target bioreactors to culture according to culture parameters, and controlling the liquid changing device to discharge the culture medium in the target bioreactors after the culture is finished. The system can reduce the dependence on manual operation in the plant tissue culture process, improve the efficiency and consistency of plant tissue culture, realize batch and large-scale production of the reactor and further ensure the sterility of the production environment.

Embodiment two:

in order to effectively exchange air in the bioreactor and provide a sterile air environment, another plant tissue culture control system is provided in this embodiment on the basis of the first embodiment.

FIG. 4 is a schematic diagram of another plant tissue culture control system. As can be seen from fig. 4, the system adds a ventilation device on the basis of the original control system. The ventilation device is connected with the control center module and is used for providing a sterile environment in the culture process. Specifically, when the control center module receives a control instruction sent by the control center module, air in the target bioreactor is subjected to gas exchange according to the control instruction, so that the sterility of the environment outside the bioreactor in the culture process is ensured. Wherein the ventilation means may be a fan, pump or other suitable device for driving the flow of air. And, the ventilation device can adopt different gas exchange modes, such as mechanical sterile ventilation, sterile gas blowing and the like.

In one embodiment, the ventilation device may be a filter fan unit. The filtering fan unit is used for filtering particulate matters and microorganisms in the air so as to send the filtered air into the external environment of the bioreactor for gas exchange. Here, the filter fan unit filters particulate matter and microorganisms in the air by filtering. In practice, the filter fan unit may be equipped with different types of filters, such as HEPA filters, uv sterilizers, etc., to improve the filtering effect and to kill microorganisms.

Thus, in the plant tissue culture control system provided in this embodiment, by additionally providing a ventilation device, for example, the filtering fan unit of the ventilation device may be an FFU air purifier, and the purifier blows purified external air into the external environment of the reactor at a high speed, so as to replace the air outside the reactor, thereby ensuring sterility.

Embodiment III:

in order to optimize the soaking effect in the culture process and realize accurate regulation and control of soaking, on the basis of the first embodiment, the embodiment provides another plant tissue culture control system.

As shown in FIG. 5, a schematic diagram of another plant tissue culture control system is provided, which adds an accurate soaking control module based on the original control system to optimize the soaking step in the culture process. The accurate soaking regulation and control module is connected with the control center module.

In actual operation, the control center module calculates the soaking preparation time of the current culture batch; wherein the soaking preparation time period comprises a flushing pipe time period for cleaning by using the new culture medium and a flowing time period for the new culture medium to flow to the target bioreactor. The control center module sends the calculated soaking preparation time to the accurate soaking regulation and control module, and the accurate soaking regulation and control module controls the bioreactor to execute a preparation process before starting soaking before reaching a soaking time point based on the soaking preparation time; and receiving a control instruction of the control center module, and automatically controlling liquid inlet and liquid outlet of the bioreactor to realize automatic filling and discharging of the culture medium. Here, the fine soaking regulation module controls the bioreactor to perform the cleaning and flowing process of the new medium before reaching the soaking time point, ensuring that the bioreactor is ready at the soaking time point. And the accurate soaking regulation and control module receives control instructions sent by the control center module, and the control instructions comprise a liquid inlet instruction and a liquid outlet instruction. The accurate soaking regulation and control module controls the opening of the liquid inlet electromagnetic valve according to the received liquid inlet instruction, so that the culture medium flows into the bioreactor from the storage container; according to the received liquid outlet instruction, the accurate soaking regulation and control module controls the liquid outlet electromagnetic valve to be opened, so that the culture medium flows out of the bioreactor to the waste treatment system.

For example, assume that the current culture batch needs to be soaked with a new medium, and that the preset soaking time point is 3 hours after the start of culture. The control center module calculates the soaking preparation time length to be 30 minutes (including the flushing pipe time length and the flowing time length) according to preset culture parameters and actual running conditions. Before the soaking time point, the accurate soaking regulation and control module controls the bioreactor to carry out the cleaning and flowing process of the new culture medium, so that the bioreactor is ensured to be ready at the soaking time point. When the control center module sends a liquid inlet instruction, the accurate soaking regulation and control module controls the liquid inlet electromagnetic valve to be opened, so that the culture medium flows into the bioreactor from the storage container; when the control center module sends a liquid outlet instruction, the accurate soaking regulation and control module controls the liquid outlet electromagnetic valve to be opened, so that the culture medium flows out of the bioreactor to the waste treatment system. Through the process, the automatic filling and discharging of the culture medium are realized, and the efficiency and the stability of plant tissue culture are improved.

The plant tissue culture control system provided by the embodiment is additionally provided with the accurate soaking regulation and control module, and the accurate soaking regulation and control module can realize automatic filling and discharging of the culture medium and realize accurate soaking regulation and control, so that the efficiency and stability of plant tissue culture are improved.

Embodiment four:

in order to ensure the safety and reliability of the cultivation process, another plant tissue culture control system is provided in this embodiment on the basis of the first embodiment.

As shown in FIG. 6, a schematic diagram of another plant tissue culture control system is provided, wherein an alarm intervention module is added on the basis of the original control system, and the alarm intervention module is connected with a control center module.

In actual operation, the control center module monitors the running state of each device in the plant tissue culture control system, and when abnormal running of the device is monitored, an alarm instruction is sent to the alarm intervention module; and when the alarm intervention module receives the alarm instruction, an alarm prompt is sent out to prompt personnel to perform human intervention treatment.

In a specific embodiment, the control center module is responsible for monitoring the operational status of various devices in the plant tissue culture control system, such as the bioreactor, the media delivery system, the temperature and humidity control system, and the like. When the abnormal operation of the equipment is monitored, if the abnormal operation exceeds the preset operation parameter range, the control center module sends a warning instruction to the warning intervention module. And the alarm intervention module immediately sends out an alarm prompt after receiving the alarm instruction, for example, through an audible alarm and/or a visual alarm lamp, so as to prompt personnel to perform human intervention treatment. Here, the alarm intervention module may also adopt other alarm modes, such as a short message alarm, an email alarm, etc. In addition, the alarm intervention module can also be added with other functions, such as automatically cutting off power supply, starting standby equipment and the like, so as to further improve the safety and reliability of the system.

According to the plant tissue culture control system provided by the embodiment, the alarm intervention module is additionally arranged, and when an alarm instruction is received, an alarm prompt is sent out to prompt personnel to perform human intervention treatment, so that the safety and reliability of the system can be improved.

Fifth embodiment:

the embodiment provides a plant tissue culture device, as shown in fig. 7, which is a schematic structural diagram, wherein the plant tissue culture device comprises the plant tissue culture control system of any one of the previous embodiments, and further comprises a plurality of bioreactors; wherein, each bioreactor is connected with a liquid exchange device in the plant tissue culture control system.

In practice, a plurality of bioreactors associated with one another in the same culture batch are usually combined into a reaction chamber, and the control of the reaction chamber is used to achieve an automated cultivation of plant cultures. The reaction chamber is an aggregate of bioreactors, capable of housing a plurality of bioreactors and providing them with the necessary environmental conditions. FIG. 8 is a schematic structural view of a plant tissue culture device based on reaction chamber control, in this embodiment, the plant tissue culture device comprises a plurality of reaction chambers 2, each reaction chamber 2 is composed of a plurality of bioreactors 1, the reaction chambers 2 are all arranged on shelves, and the shelves are transparent; each reaction chamber 2 is provided with an independent upturned door 3, which facilitates the placement and extraction of the bioreactor 1. In addition, a control screen 4 is arranged on the side surface of the layer frame, namely, the control center module in the embodiment is an industrial control integrated machine with a touch screen, so that a user can conveniently input culture parameters, observe the culture progress of the current batch and the frequency of liquid change. And, a filtering fan unit 5 is also arranged above the layer frame, namely, corresponds to the ventilation device in the foregoing description, and is used for providing gas exchange of the filtered sterile air, so as to ensure the sterile environment in the reaction chamber 2.

The arrangement of the bioreactors inside the reaction chambers is shown in fig. 9, where the bioreactors are arranged in a certain order and manner. For example, they may be placed on shelves 7, each shelf 7 placing a plurality of bioreactors as required. Each bioreactor is connected with a corresponding liquid inlet pipeline and a corresponding liquid outlet pipeline so as to facilitate the input and the discharge of the culture medium. In one embodiment, a reaction chamber may include 72 bioreactors, each with separate inlet and outlet control valves.

FIG. 10 is a schematic view showing the structure of the piping inside the reaction chamber, which in this embodiment includes an air pump piping and a medium piping. The air pump pipeline mainly comprises an air pump 6, an air bottle, a liquid inlet valve, a liquid outlet valve, a hose and the like and is used for controlling the inlet and outlet of air and the flow of a culture medium. The medium line is responsible for transporting the medium to each bioreactor and for discharging the waste liquid from the bioreactor. Here, the design of the tubing is required to ensure accurate control of the flow and pressure of the medium, as well as to ensure aseptic operation. In one of the pipe structures, as shown in fig. 11, a connection structure of a connection pipe and a joint terminal is schematically shown, wherein the connection pipe 8 includes a gas connection pipe and a liquid connection pipe, and when a liquid exchange operation is required, the joint terminal 9 of the culture liquid can be connected with a terminal on the layer rack, so that automatic liquid exchange is realized. Here, each bioreactor has a corresponding connector terminal 9, which ensures that each reactor can be controlled independently, while also facilitating maintenance and cleaning. In one embodiment, referring to fig. 12, a schematic structural diagram of a bioreactor is provided, and an air inlet, an air outlet, a liquid inlet valve, a liquid outlet valve and a waste liquid treatment valve are arranged on the side surface of the bioreactor. Wherein, the specification of the bioreactor 1 is matched with the culture source and the target culture height and size of the culture source, and the size of the reaction cabin is also matched with the height and size of each layer of bioreactor 1.

The plant tissue culture device provided by the embodiment of the invention has the same technical characteristics as the plant tissue culture control system provided by the embodiment, so that the same technical problems can be solved, and the same technical effects can be achieved.

Example six:

the embodiment of the invention also provides a plant tissue culture control method which is applied to the plant tissue culture control system in any of the previous embodiments. As shown in fig. 13, a flow chart of a plant tissue culture control method is shown, and the method comprises the following steps:

step S102: obtaining culture parameters of a current culture batch; the culture parameters include culture time, soaking interval and soaking time.

For example, the experimenter may input the total incubation time required for the current incubation lot, the interval time of each soak, and the length of time of each soak on the upper computer (i.e., the control center module).

Step S104: a target bioreactor associated with the current culture batch is determined.

For example, the upper level may calculate a list of bioreactors to be soaked based on the culture parameters entered by the experimenter and send a signal to these bioreactors that the current batch has started.

Step S106: the target bioreactor is cleaned by the liquid exchanging device to discharge the waste liquid.

For example, the upper level can control the liquid inlet valve and the liquid outlet valve of the related bioreactor, and the waste liquid in the bioreactor is discharged after being washed clean by using a new culture medium.

Step S108: and discharging a new culture medium corresponding to the current culture batch into the target bioreactor through the liquid exchange device.

For example, the upper level can control the liquid inlet valve of the related bioreactor, and new culture medium is filled into the bioreactor according to a set volume.

Step S110: the target bioreactor is controlled to culture according to the culture parameters.

For example, the upper computer calculates the beginning and ending time of each soaking according to the culture parameters input by the experimenter, and controls the liquid inlet valve and the liquid outlet valve of the bioreactor at the soaking time point to perform the soaking operation.

Step S112: after the completion of the culture, the medium in the target bioreactor is discharged by the liquid exchange device.

For example, the upper level can control the liquid outlet valve of the related bioreactor after the culture is finished, so as to empty the culture medium in the bioreactor.

According to the plant tissue culture control method provided by the embodiment, the control system is used for automatically controlling and completing the culture of plant tissues, and for multiple soaking of one culture batch, the whole process is automatically controlled and completed by the system without manual participation. The method can reduce the dependence on manual operation in the plant tissue culture process, improve the efficiency and consistency of plant tissue culture, realize batch and large-scale production of the reactor and further ensure the sterility of the production environment.

In another possible embodiment, when the plant tissue culture control system includes the precise soaking control module, the control method further includes the following control procedures in addition to the control procedure shown in fig. 13:

(1) Before starting soaking, the upper computer calculates the time length of soaking preparation and sends the time length to the accurate soaking regulation and control module, wherein the time length comprises the time length of using a new culture medium to wash a pipe in a pipeline, the time length of flowing the culture medium and the like; the accurate soaking regulation and control module controls the preparation process before the reactor starts soaking in advance.

(2) And after the soaking time point is reached, controlling a liquid inlet valve of the bioreactor, and filling a set volume of culture medium into the bioreactor.

(3) After the soaking is finished, a liquid outlet valve of the bioreactor is controlled, and the culture medium in the reactor is emptied through air pressure.

In the whole soaking process, the accurate soaking regulation and control module realizes the automation and the accurate control of the culture medium replacement in the plant tissue culture process through the connected components and the accurate control algorithm, improves the culture efficiency and the success rate, and reduces the complexity of manual operation and the dependence on personnel.

In another possible embodiment, when the plant tissue culture control system includes an alarm intervention module, the control method further includes, in addition to the control flow shown in fig. 13, the following control flow:

(1) The upper computer continuously monitors the running state of each device.

(2) And once the abnormal operation of the equipment is detected, the upper computer immediately activates an alarm intervention module.

(3) The alarm intervention module gives an alarm in the modes of sound, light, screen display and the like to inform operators. After receiving the alarm signal, the operator can rapidly intervene in the process, such as checking equipment, replacing parts, adjusting parameters and the like, so as to ensure the continuity and stability of the culture process.

Therefore, the safety and the reliability of the plant tissue culture control method are enhanced through the participation of the alarm intervention module, and any potential problems can be timely found and effectively treated in the automatic operation process.

The computer program product of the plant tissue culture control method provided by the embodiment of the invention comprises a computer readable storage medium storing program codes, wherein the instructions included in the program codes can be used for executing the plant tissue culture control method in the previous method embodiment, and specific implementation can be seen in the method embodiment and will not be described herein.

The functions, if implemented in the form of software functional units and sold or used as a stand-alone product, may be stored in a non-volatile computer readable storage medium executable by a processor. Based on this understanding, the technical solution of the present invention may be embodied essentially or in a part contributing to the prior art or in a part of the technical solution, in the form of a software product stored in a storage medium, comprising several instructions for causing a computer device (which may be a personal computer, a server, a network device, etc.) to perform all or part of the steps of the method according to the embodiments of the present invention. And the aforementioned storage medium includes: a U-disk, a removable hard disk, a Read-Only Memory (ROM), a random access Memory (RAM, random Access Memory), a magnetic disk, or an optical disk, or other various media capable of storing program codes.

In addition, in the description of embodiments of the present invention, unless explicitly stated and limited otherwise, the terms "mounted," "connected," and "connected" are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; can be directly connected or indirectly connected through an intermediate medium, and can be communication between two elements. The specific meaning of the above terms in the present invention will be understood in specific cases by those of ordinary skill in the art.

In the description of the present invention, it should be noted that the directions or positional relationships indicated by the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc. are based on the directions or positional relationships shown in the drawings, are merely for convenience of describing the present invention and simplifying the description, and do not indicate or imply that the devices or elements referred to must have a specific orientation, be configured and operated in a specific orientation, and thus should not be construed as limiting the present invention. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.

Finally, it should be noted that: the above examples are only specific embodiments of the present invention, and are not intended to limit the scope of the present invention, but it should be understood by those skilled in the art that the present invention is not limited thereto, and that the present invention is described in detail with reference to the foregoing examples: any person skilled in the art may modify or easily conceive of the technical solution described in the foregoing embodiments, or perform equivalent substitution of some of the technical features, while remaining within the technical scope of the present disclosure; such modifications, changes or substitutions do not depart from the spirit and scope of the technical solutions of the embodiments of the present invention, and are intended to be included in the scope of the present invention. Therefore, the protection scope of the present invention shall be subject to the protection scope of the claims.

Claims (11)

1. The plant tissue culture control system is characterized by comprising a control center module, a reactor control unit and a liquid exchange device which are connected in sequence; the liquid exchange device is connected with a plurality of peripheral bioreactors;

the control center module is used for acquiring culture parameters of the current culture batch and sending the culture parameters to the reactor control unit; the culture parameters comprise culture time, soaking interval and soaking time;

The reactor control unit is used for determining a target bioreactor associated with the current culture batch from the plurality of bioreactors, controlling the liquid changing device to clean the target bioreactor so as to discharge waste liquid, discharging new culture medium corresponding to the current culture batch into the target bioreactor, controlling the target bioreactor to culture according to the culture parameters, and controlling the liquid changing device to discharge the culture medium in the target bioreactor after the culture is finished.

2. The plant tissue culture control system according to claim 1, wherein the liquid exchanging device comprises a liquid inlet valve, a liquid outlet valve and a liquid exchanging power unit;

the liquid inlet valve and the liquid outlet valve are both arranged on the bioreactor;

the hydrodynamic force changing unit is used for providing hydrodynamic force for discharging the culture medium into or out of the bioreactor.

3. The plant tissue culture control system according to claim 2, wherein the hydraulic power changing unit comprises an air pump, an air bottle and a connecting pipeline;

the air pump is connected with the air bottle, and is also connected with the liquid inlet valve and the liquid outlet valve through the connecting pipelines respectively;

The air pump is used for discharging the culture medium into or out of the bioreactor through air pressure.

4. The plant tissue culture control system according to claim 2, wherein the liquid changing device further comprises a waste liquid treatment valve; the waste liquid treatment valve is arranged on the bioreactor; the waste liquid treatment valve is connected with the reactor control unit;

the waste liquid treatment valve is used for receiving an opening instruction of the reactor control unit and opening the valve to discharge waste liquid in the bioreactor; and the device is also used for receiving a closing instruction of the reactor control unit, and closing a valve to prevent waste liquid from flowing back to the bioreactor.

5. The plant tissue culture control system of claim 1, wherein the control system further comprises a ventilation device; the air interchanger is connected with the control center module;

the ventilation device is used for receiving the control instruction sent by the control center module, and exchanging air outside the target bioreactor according to the control instruction so as to ensure the sterility of the environment outside the target bioreactor in the culture process.

6. The plant tissue culture control system of claim 1, further comprising a precision soaking regulation module, the precision soaking regulation module being connected to the control center module;

The control center module is also used for calculating the soaking preparation time of the current culture batch; the soaking preparation time period comprises a flushing pipe time period for cleaning by using the new culture medium and a flowing time period for the new culture medium to flow to the target bioreactor;

the accurate soaking regulation and control module is used for controlling the bioreactor to execute a preparation process before soaking starts before reaching a soaking time point based on the soaking preparation time; and receiving a control instruction of the control center module, and automatically controlling liquid inlet and liquid outlet of the bioreactor so as to realize automatic filling and discharging of the culture medium.

7. The plant tissue culture control system of claim 1, further comprising an alarm intervention module coupled to the control center module;

the control center module is also used for monitoring the running state of each device in the plant tissue culture control system, and sending a warning instruction to the warning intervention module when abnormal running of the device is monitored;

the alarm intervention module is used for sending out an alarm prompt when receiving the alarm instruction so as to prompt personnel to perform human intervention treatment.

8. The plant tissue culture control system according to claim 1, wherein the control center module comprises a human-computer interaction unit and a display unit;

the human-computer interaction unit is used for providing a human-computer interaction interface and receiving a user input instruction;

the display unit is used for displaying the running state and the liquid change information of the control system.

9. The plant tissue culture control system according to any one of claims 1-8, wherein the control center module is an upper computer and the reactor control unit is a PLC programmable logic controller.

10. A plant tissue culture apparatus comprising the plant tissue culture control system of any one of claims 1-9, further comprising a plurality of bioreactors;

each bioreactor is connected with a liquid exchange device in the plant tissue culture control system.

11. A plant tissue culture control method, characterized by being applied to the plant tissue culture control system according to any one of claims 1 to 9; the method comprises the following steps:

obtaining culture parameters of a current culture batch; the culture parameters comprise culture time, soaking interval and soaking time;

Determining a target bioreactor associated with the current culture batch;

cleaning the target bioreactor by a liquid exchange device to discharge waste liquid;

discharging a new culture medium corresponding to the current culture batch into the target bioreactor through a liquid exchange device;

controlling the target bioreactor to culture according to the culture parameters;

after the culture is finished, the culture medium in the target bioreactor is discharged through the liquid exchange device.