CN1177938C - 选择性连接及扩增方法 - Google Patents

选择性连接及扩增方法 Download PDF

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Publication number
CN1177938C
CN1177938C CNB98804157XA CN98804157A CN1177938C CN 1177938 C CN1177938 C CN 1177938C CN B98804157X A CNB98804157X A CN B98804157XA CN 98804157 A CN98804157 A CN 98804157A CN 1177938 C CN1177938 C CN 1177938C
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primer
segment
sequence
reaction mixture
substantially complementary
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Chinese (zh)
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CN1252104A (zh
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����ɭ��Τ�������е�
艾利森·韦利安·托德
卡罗琳·简·富尔瑞
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Johnson and Johnson Research Pty Ltd
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Johnson and Johnson Research Pty Ltd
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CNB98804157XA 1997-02-21 1998-02-23 选择性连接及扩增方法 Expired - Fee Related CN1177938C (zh)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
AUPO5248A AUPO524897A0 (en) 1997-02-21 1997-02-21 Method of amplifying specific nucleic acid target sequences
AUPO5248 1997-02-21

Publications (2)

Publication Number Publication Date
CN1252104A CN1252104A (zh) 2000-05-03
CN1177938C true CN1177938C (zh) 2004-12-01

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ID=3799551

Family Applications (1)

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CNB98804157XA Expired - Fee Related CN1177938C (zh) 1997-02-21 1998-02-23 选择性连接及扩增方法

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US (1) US6245505B1 (enExample)
EP (1) EP1002124A4 (enExample)
JP (1) JP2001517936A (enExample)
KR (1) KR20000075538A (enExample)
CN (1) CN1177938C (enExample)
AU (1) AUPO524897A0 (enExample)
BR (1) BR9807253A (enExample)
CA (1) CA2282733A1 (enExample)
WO (1) WO1998037230A1 (enExample)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104372093A (zh) * 2014-11-10 2015-02-25 博奥生物集团有限公司 一种基于高通量测序的snp检测方法

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6893877B2 (en) 1998-01-12 2005-05-17 Massachusetts Institute Of Technology Methods for screening substances in a microwell array
WO2000056456A1 (en) 1999-03-19 2000-09-28 Genencor International, Inc. Multi-through hole testing plate for high throughput screening
US20060275782A1 (en) 1999-04-20 2006-12-07 Illumina, Inc. Detection of nucleic acid reactions on bead arrays
US7582420B2 (en) 2001-07-12 2009-09-01 Illumina, Inc. Multiplex nucleic acid reactions
US7611869B2 (en) 2000-02-07 2009-11-03 Illumina, Inc. Multiplexed methylation detection methods
EP1130113A1 (en) 2000-02-15 2001-09-05 Johannes Petrus Schouten Multiplex ligation dependent amplification assay
US20020151040A1 (en) 2000-02-18 2002-10-17 Matthew O' Keefe Apparatus and methods for parallel processing of microvolume liquid reactions
AU2001265121A1 (en) * 2000-05-30 2001-12-11 Applera Corporation Methods for detecting target nucleic acids using coupled ligation and amplification
US7838270B2 (en) * 2001-05-22 2010-11-23 The University Of Chicago Target-dependent transcription using deletion mutants of N4 RNA polymerase
US8277753B2 (en) 2002-08-23 2012-10-02 Life Technologies Corporation Microfluidic transfer pin
CA2521999A1 (en) 2002-12-20 2004-09-02 Biotrove, Inc. Assay apparatus and method using microfluidic arrays
US20050130213A1 (en) * 2003-12-10 2005-06-16 Tom Morrison Selective ligation and amplification assay
CA2549849A1 (en) * 2003-12-10 2005-06-30 Biotrove, Inc. Improved selective ligation and amplification assay
WO2005089945A1 (en) 2004-03-12 2005-09-29 Biotrove, Inc. Nanoliter array loading
US20050282195A1 (en) * 2004-04-30 2005-12-22 Applera Corporation Compositions, methods, and kits for (MIS)ligating oligonucleotides
WO2007040592A1 (en) * 2005-03-10 2007-04-12 Gao, Xiaolian Making nucleic acid sequences in parallel and use
CN100487432C (zh) * 2006-06-22 2009-05-13 上海交通大学 采用分子信标对核酸信号进行恒温放大与检测的方法

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6020124A (en) 1992-04-27 2000-02-01 Trustees Of Dartmouth College Detection of soluble gene sequences in biological fluids
WO1994017210A1 (en) 1993-01-27 1994-08-04 Oncor, Inc. Amplification of nucleic acid sequences
US5593840A (en) 1993-01-27 1997-01-14 Oncor, Inc. Amplification of nucleic acid sequences
IL105894A (en) * 1993-06-03 1997-02-18 Asher Nathan Method for the detection of nucleic acid sequence by using a detection ensemble and an amplification ensemble
EP0777749B1 (en) * 1994-08-19 2002-10-30 PE Corporation (NY) Coupled amplification and ligation method
WO1996015271A1 (en) * 1994-11-16 1996-05-23 Abbott Laboratories Multiplex ligations-dependent amplification
JPH11506605A (ja) * 1995-06-07 1999-06-15 アボツト・ラボラトリーズ 増幅反応におけるバックグラウンドを減少させるためにプローブをマスキングする方法
CA2199213C (en) * 1996-03-11 2007-04-17 John Wesley Backus Amplifying and detecting target nucleic acids using a post amplification incubation step

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104372093A (zh) * 2014-11-10 2015-02-25 博奥生物集团有限公司 一种基于高通量测序的snp检测方法
CN104372093B (zh) * 2014-11-10 2016-09-21 博奥生物集团有限公司 一种基于高通量测序的snp检测方法
US10704092B2 (en) 2014-11-10 2020-07-07 Capitalbio Corporation Detection methods based on sequencing

Also Published As

Publication number Publication date
AUPO524897A0 (en) 1997-03-20
EP1002124A4 (en) 2004-04-14
CN1252104A (zh) 2000-05-03
KR20000075538A (ko) 2000-12-15
WO1998037230A1 (en) 1998-08-27
BR9807253A (pt) 2001-08-28
CA2282733A1 (en) 1998-08-27
JP2001517936A (ja) 2001-10-09
US6245505B1 (en) 2001-06-12
EP1002124A1 (en) 2000-05-24

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