CN117757648A - Fermentation inoculant and application thereof in production of broussonetia papyrifera feed premix - Google Patents
Fermentation inoculant and application thereof in production of broussonetia papyrifera feed premix Download PDFInfo
- Publication number
- CN117757648A CN117757648A CN202311758633.4A CN202311758633A CN117757648A CN 117757648 A CN117757648 A CN 117757648A CN 202311758633 A CN202311758633 A CN 202311758633A CN 117757648 A CN117757648 A CN 117757648A
- Authority
- CN
- China
- Prior art keywords
- parts
- broussonetia papyrifera
- fermentation
- premix
- weight
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 241000705930 Broussonetia papyrifera Species 0.000 title claims abstract description 51
- 238000000855 fermentation Methods 0.000 title claims abstract description 50
- 230000004151 fermentation Effects 0.000 title claims abstract description 50
- 239000002054 inoculum Substances 0.000 title claims abstract description 27
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 6
- 102000004190 Enzymes Human genes 0.000 claims abstract description 33
- 108090000790 Enzymes Proteins 0.000 claims abstract description 33
- 241000287828 Gallus gallus Species 0.000 claims abstract description 31
- 241000894006 Bacteria Species 0.000 claims abstract description 28
- 239000001963 growth medium Substances 0.000 claims abstract description 21
- 241000933832 Broussonetia Species 0.000 claims description 42
- 229940088598 enzyme Drugs 0.000 claims description 32
- 238000002156 mixing Methods 0.000 claims description 24
- 239000002131 composite material Substances 0.000 claims description 20
- 150000001875 compounds Chemical class 0.000 claims description 17
- 238000001035 drying Methods 0.000 claims description 17
- 244000063299 Bacillus subtilis Species 0.000 claims description 15
- 235000014469 Bacillus subtilis Nutrition 0.000 claims description 15
- 241000235646 Cyberlindnera jadinii Species 0.000 claims description 15
- 240000006024 Lactobacillus plantarum Species 0.000 claims description 15
- 235000013965 Lactobacillus plantarum Nutrition 0.000 claims description 15
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 15
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 claims description 15
- 244000057717 Streptococcus lactis Species 0.000 claims description 15
- 235000014897 Streptococcus lactis Nutrition 0.000 claims description 15
- 229940072205 lactobacillus plantarum Drugs 0.000 claims description 15
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 14
- 239000000843 powder Substances 0.000 claims description 14
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 13
- 108010011619 6-Phytase Proteins 0.000 claims description 11
- 108091005507 Neutral proteases Proteins 0.000 claims description 11
- 102000035092 Neutral proteases Human genes 0.000 claims description 11
- 229920005610 lignin Polymers 0.000 claims description 11
- 238000007873 sieving Methods 0.000 claims description 11
- 108090000145 Bacillolysin Proteins 0.000 claims description 10
- 108010059892 Cellulase Proteins 0.000 claims description 10
- 240000008042 Zea mays Species 0.000 claims description 10
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 10
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 10
- 229940106157 cellulase Drugs 0.000 claims description 10
- 235000005822 corn Nutrition 0.000 claims description 10
- 229940085127 phytase Drugs 0.000 claims description 10
- 238000000034 method Methods 0.000 claims description 9
- 238000003756 stirring Methods 0.000 claims description 9
- 240000007594 Oryza sativa Species 0.000 claims description 8
- 235000007164 Oryza sativa Nutrition 0.000 claims description 8
- 239000000463 material Substances 0.000 claims description 8
- 235000013379 molasses Nutrition 0.000 claims description 8
- 235000009566 rice Nutrition 0.000 claims description 8
- 239000011780 sodium chloride Substances 0.000 claims description 7
- 235000015099 wheat brans Nutrition 0.000 claims description 7
- 235000013312 flour Nutrition 0.000 claims description 6
- 230000001954 sterilising effect Effects 0.000 claims description 6
- 239000003795 chemical substances by application Substances 0.000 claims description 5
- 238000010299 mechanically pulverizing process Methods 0.000 claims description 5
- 238000010298 pulverizing process Methods 0.000 claims description 2
- 239000002609 medium Substances 0.000 claims 1
- 235000013330 chicken meat Nutrition 0.000 abstract description 20
- 235000019750 Crude protein Nutrition 0.000 abstract description 7
- 239000000835 fiber Substances 0.000 abstract description 7
- 108090000765 processed proteins & peptides Proteins 0.000 abstract description 6
- 150000004676 glycans Chemical class 0.000 abstract description 5
- 229920001282 polysaccharide Polymers 0.000 abstract description 5
- 239000005017 polysaccharide Substances 0.000 abstract description 5
- 238000011161 development Methods 0.000 abstract description 4
- 235000016709 nutrition Nutrition 0.000 abstract description 3
- 230000000975 bioactive effect Effects 0.000 abstract description 2
- 238000009395 breeding Methods 0.000 abstract description 2
- 230000001488 breeding effect Effects 0.000 abstract description 2
- 230000000813 microbial effect Effects 0.000 abstract description 2
- 230000002195 synergetic effect Effects 0.000 abstract 1
- 238000002360 preparation method Methods 0.000 description 8
- 238000012360 testing method Methods 0.000 description 6
- 235000021050 feed intake Nutrition 0.000 description 5
- 239000003153 chemical reaction reagent Substances 0.000 description 4
- 239000000203 mixture Substances 0.000 description 4
- 239000002994 raw material Substances 0.000 description 4
- 239000007858 starting material Substances 0.000 description 4
- 150000001413 amino acids Chemical class 0.000 description 3
- 235000013339 cereals Nutrition 0.000 description 3
- 235000019784 crude fat Nutrition 0.000 description 3
- 244000144972 livestock Species 0.000 description 3
- 239000002068 microbial inoculum Substances 0.000 description 3
- 235000015097 nutrients Nutrition 0.000 description 3
- 235000014590 basal diet Nutrition 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 230000029087 digestion Effects 0.000 description 2
- 238000009313 farming Methods 0.000 description 2
- 235000012054 meals Nutrition 0.000 description 2
- 244000144977 poultry Species 0.000 description 2
- 235000013594 poultry meat Nutrition 0.000 description 2
- 239000001648 tannin Substances 0.000 description 2
- 235000018553 tannin Nutrition 0.000 description 2
- 229920001864 tannin Polymers 0.000 description 2
- 238000005303 weighing Methods 0.000 description 2
- 244000144730 Amygdalus persica Species 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- 108010084185 Cellulases Proteins 0.000 description 1
- 102000005575 Cellulases Human genes 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 206010025476 Malabsorption Diseases 0.000 description 1
- 208000004155 Malabsorption Syndromes Diseases 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 244000046052 Phaseolus vulgaris Species 0.000 description 1
- 235000010627 Phaseolus vulgaris Nutrition 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- 235000006040 Prunus persica var persica Nutrition 0.000 description 1
- 235000019764 Soybean Meal Nutrition 0.000 description 1
- 238000000692 Student's t-test Methods 0.000 description 1
- 230000001133 acceleration Effects 0.000 description 1
- 238000000540 analysis of variance Methods 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000000433 anti-nutritional effect Effects 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 201000006549 dyspepsia Diseases 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000003797 essential amino acid Substances 0.000 description 1
- 235000020776 essential amino acid Nutrition 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 230000003031 feeding effect Effects 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 230000003053 immunization Effects 0.000 description 1
- 238000002649 immunization Methods 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 108010062085 ligninase Proteins 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 235000019629 palatability Nutrition 0.000 description 1
- OQUKIQWCVTZJAF-UHFFFAOYSA-N phenol;sulfuric acid Chemical compound OS(O)(=O)=O.OC1=CC=CC=C1 OQUKIQWCVTZJAF-UHFFFAOYSA-N 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 239000004455 soybean meal Substances 0.000 description 1
- 238000007619 statistical method Methods 0.000 description 1
- 210000002784 stomach Anatomy 0.000 description 1
- QAOWNCQODCNURD-UHFFFAOYSA-N sulfuric acid Substances OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 1
- 238000012353 t test Methods 0.000 description 1
- 229960005486 vaccine Drugs 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Landscapes
- Fodder In General (AREA)
Abstract
The invention discloses a fermentation inoculant and application thereof in production of broussonetia papyrifera feed premix, and belongs to the technical field of microbial fermentation feeds. According to the invention, the fermentation inoculant is inoculated into the broussonetia papyrifera leaf culture medium, the synergistic effect of bacteria and enzymes can be fully exerted through fermentation, the content of crude protein, active polysaccharide and active peptide is improved, the content of crude fiber is reduced, the nutritional value of premix is improved, the utilization rate of the broussonetia papyrifera feed by chickens is improved, the requirements of chickens on high-quality crude protein and bioactive peptide are met, and the development of chicken breeding industry is promoted.
Description
Technical Field
The invention belongs to the field of microbial fermentation feeds, and particularly relates to a fermentation microbial inoculum and application thereof in production of broussonetia papyrifera feed premix.
Background
The paper mulberry (academic name Broussonetia papyrifera) aliases such as papermulberry peach is a deciduous arbor and has the characteristics of fast growth, strong adaptability, wide distribution, easy propagation, high heat quantity and short rotation period. The protein content of the broussonetia papyrifera leaves is up to 20% -30%, and the nutrient components such as amino acid, vitamin, carbohydrate and microelements are also very rich, so that the broussonetia papyrifera leaves can be used for producing complete livestock and poultry feeds after scientific processing. The crude fat content of the paper mulberry is 1.58-5.31 percent, which is higher than that of corn and soybean meal. The paper mulberry leaves in the paper mulberry are rich in amino acids, 7 kinds of essential amino acids and more than 20% of total amino acids, and the calcium and phosphorus contents of the paper mulberry are respectively 2.23-3.64% and 0.3-0.61%. Therefore, the paper mulberry is used for replacing part of grain feed, which is beneficial to alleviating the problem of grain contention, and further promotes the high-speed development of the livestock industry in China. However, the protein structure of paper mulberry is complex, the content of crude fiber and lignin is high, and the paper mulberry contains anti-nutritional factors such as tannin, so that the direct feeding can cause dyspepsia and malabsorption of livestock and poultry, and the feeding effect is reduced, so that paper mulberry is necessary to be processed and utilized.
The current method for directly silaging and fermenting the paper mulberry is to take 70% of whole paper mulberry powder (crushed by a 20-60 sieve) and 30% of other raw materials (bran l0%, corn powder 5%, rice chaff 5% and molasses l 0%) as fermentation raw materials, uniformly mix the raw materials with water about 60%, fill the mixture into a fermentation bag for sealing, and ferment for 20-30d at 35 ℃, but the main problem of poor palatability caused by high content of crude fiber and tannin of paper mulberry itself is that the paper mulberry feed is obtained by natural fermentation.
With the acceleration of the Chinese farming industry, the farming amount is greatly improved, and the difficult situation of 'competing for grain' is formed. In order to alleviate this contradiction between supply and demand, development efforts of woody feed (such as paper mulberry) resources are urgently needed. Therefore, the research of the invention provides a fermentation inoculant and application thereof in the broussonetia papyrifera feed premix, and has important significance in improving the added value of broussonetia papyrifera and developing a novel broussonetia papyrifera feed premix.
Disclosure of Invention
The invention aims to: the invention aims to provide a broussonetia papyrifera leaf fermentation inoculant for improving the added value of broussonetia papyrifera leaves.
The invention also solves the technical problem of providing the application of the fermentation inoculant in the broussonetia papyrifera feed premix, and provides a novel broussonetia papyrifera feed premix for the field of chicken cultivation.
The technical scheme is as follows: in order to solve the technical problems, the invention provides the following technical scheme:
the fermenting bacteria agent comprises compound bacteria and compound enzyme, wherein the mass ratio of the compound bacteria to the compound enzyme is (15-55): (25-60); preferably, the mass ratio of the complex bacteria to the complex enzyme is 51:49;
the composite bacteria comprise the following components in parts by weight: 10-20 parts of saccharomyces cerevisiae, 5-15 parts of lactobacillus plantarum, 10-25 parts of bacillus subtilis, 5-10 parts of candida utilis and 15-25 parts of streptococcus lactis, preferably 12 parts of saccharomyces cerevisiae, 9 parts of lactobacillus plantarum, 18 parts of bacillus subtilis, 70 parts of candida utilis and 19 parts of streptococcus lactis;
the complex enzyme comprises the following components in parts by weight: 5-20 parts of cellulase, 10-15 parts of phytase, 5-15 parts of lignin enzyme and 1-8 parts of neutral protease, preferably 18 parts of cellulase, 11 parts of phytase, 10 parts of lignin enzyme and 6 parts of neutral protease;
further, the saccharomyces cerevisiae is saccharomyces cerevisiae ATCC9080; the lactobacillus plantarum is lactobacillus plantarum 22811; the bacillus subtilis is bacillus subtilis CMCC (B) 63501; the candida utilis is candida utilis ATCC22023; the streptococcus lactis ACCC11093 is streptococcus lactis ACCC11093.
The preparation method of the fermentation inoculant comprises the following steps:
(1) Preparing materials: mixing 5-20 parts by weight of cellulase, 10-15 parts by weight of phytase, 5-15 parts by weight of lignin enzyme and 1-8 parts by weight of neutral protease to prepare a compound enzyme for standby;
mixing 10-20 parts of saccharomyces cerevisiae, 5-15 parts of lactobacillus plantarum, 10-25 parts of bacillus subtilis, 5-10 parts of candida utilis and 15-25 parts of streptococcus lactis according to parts by weight to prepare a composite bacterium for standby;
(2) Mixing: the composite bacteria and the composite enzyme are mixed according to the mass ratio of (15-55): (25-60) and stirring for 5min at 20 ℃ and stirring speed of 60r/min to obtain the fermentation inoculum.
The application of the fermentation inoculant in the production of the broussonetia papyrifera feed premix is within the protection scope of the invention.
The method for preparing the broussonetia papyrifera leaf chicken feed premix by using the fermentation inoculant comprises the following steps:
(1) Crushing broussonetia papyrifera leaves: drying fresh folium Broussonetiae at 20-30deg.C for 5-10h, mechanically pulverizing, and sieving with 40 mesh sieve to obtain folium Broussonetiae powder;
(2) Preparing a paper mulberry culture medium: uniformly mixing 45-65 parts of broussonetia papyrifera leaf powder, 10-25 parts of corn flour, 3-5 parts of wheat bran, 5-7 parts of rice bran, 5-15 parts of molasses and 0.2-0.4 part of sodium chloride according to parts by weight, and sterilizing at 121 ℃ for 20min to obtain a broussonetia papyrifera culture medium; preferably, the broussonetia papyrifera leaf powder 60, the corn meal 18, the wheat bran 4, the rice bran 6, the molasses 11.7 and the sodium chloride 0.3;
(3) Inoculating and fermenting: inoculating a fermentation inoculant into a paper mulberry culture medium, wherein the mass ratio of the fermentation inoculant to the paper mulberry culture medium is (5-15): 100, fermenting for 48-84 hours at the temperature of 25-35 ℃ to obtain premix;
(4) And (3) drying: drying the premix at 30-50deg.C, wherein the mass content of water in the dried premix is less than 5%, pulverizing, and sieving with 40-60 mesh sieve to obtain the folium Broussonetiae chicken feed premix.
The beneficial effects are that:
the invention prepares the cellulase, the phytase, the lignin enzyme and the neutral protease into compound enzyme, prepares the saccharomyces cerevisiae, lactobacillus plantarum, bacillus subtilis, candida utilis and streptococcus lactis into compound bacteria, prepares the compound enzyme and the compound bacteria into a fermentation microbial inoculum, inoculates the fermentation microbial inoculum into a broussonetia papyrifera leaf culture medium, can fully exert the synergism of the bacteria and the enzyme through fermentation, reduces the content of cellulose in the broussonetia papyrifera leaf culture medium, improves the content of crude protein, active polysaccharide and bioactive peptide in the premix, improves the nutritive value of the premix, and improves the utilization efficiency of chickens on broussonetia papyrifera feeds.
Detailed Description
The technical scheme of the invention is further described below with reference to the specific embodiments.
The invention adopts the materials: fresh broussonetia papyrifera leaves (obtained from Zhujin county, guizhou) are commercial products, and the corn flour, the wheat bran, the bean pulp, the rice bran and the like meet the relevant standards of national feed raw materials.
Cellulases, phytases, ligninases, neutral proteases (novelin (china) investment limited).
Saccharomyces cerevisiae ATCC9080 (Shanghai, biotechnology Co., ltd.), lactobacillus plantarum 22811 (Shanghai, micro-technology Co., ltd.), bacillus subtilis CMCC (B) 63501 (Beijing Santa Classification Co., ltd.), candida utilis ATCC22023 (Shanghai, biotechnology Co., ltd.), streptococcus lactis ACCC11093 (Shanghai, biotechnology Co., ltd.).
The reagents used in the present invention: glucose, concentrated sulfuric acid, etc. are all reagents of homemade analytical purity.
The apparatus and device used in the present invention: DJ-04 pulverizer, HVE-50 high pressure steam sterilizing pot, SWCJ-IF type clean bench, DH6000B electrothermal constant temperature incubator, HT-211B type culture shaking table. All experiments were performed in triplicate, data expressed as mean ± SD, and statistical analysis of the data using t-test or ANOVA analysis, p <0.05 and p <0.01 were considered to be statistically different.
In the following examples, all reagents, materials and equipment selected in the present invention are those well known in the art, unless otherwise specified, but are not limited to practice of the present invention, and other reagents and equipment well known in the art may be suitable for practice of the following embodiments of the present invention.
Example 1: preparation of fermentation inoculant
(1) Preparing materials: mixing cellulase 17, phytase 12, lignin enzyme 9 and neutral protease 7 according to parts by weight to prepare composite enzyme for standby; mixing saccharomyces cerevisiae 14, lactobacillus plantarum 9, bacillus subtilis 13, candida utilis 7 and streptococcus lactis 20 according to parts by weight to prepare composite bacteria for standby;
(2) Mixing: according to the mass portions, the compound bacteria 53 and the compound enzyme 47 are mixed, and stirred for 5min under the conditions of the temperature of 20 ℃ and the stirring rotation speed of 60r/min, so that the fermentation bacteria agent can be prepared.
Example 2: preparation of fermentation inoculant
(1) Preparing materials: mixing cellulase 16, phytase 13, lignin enzyme 10 and neutral protease 6 according to parts by weight to prepare composite enzyme for standby;
mixing Saccharomyces cerevisiae 16, lactobacillus plantarum 10, bacillus subtilis 15, candida utilis 6 and streptococcus lactis 18 according to parts by weight to prepare composite bacteria for standby;
(2) Mixing: mixing the complex bacteria 50 and the complex enzyme 50 according to the mass parts, and stirring for 5min at the temperature of 20 ℃ and the stirring rotation speed of 60r/min to obtain the starter.
Example 3: preparation of fermentation inoculant
(1) Preparing materials: mixing cellulase 15, phytase 11, lignin enzyme 12 and neutral protease 5 according to parts by weight to prepare composite enzyme for standby; mixing saccharomyces cerevisiae 17, lactobacillus plantarum 11, bacillus subtilis 18, candida utilis 8 and streptococcus lactis 17 according to parts by weight to prepare composite bacteria for standby;
(2) Mixing: according to the mass portion, the composite bacteria 45 and the composite enzyme 55 are mixed, and stirred for 5min under the conditions of the temperature of 20 ℃ and the stirring rotation speed of 60r/min, thus obtaining the starter.
Example 4: preparation of starter
(1) Preparing materials: mixing cellulase 18, phytase 11, lignin enzyme 10 and neutral protease 6 according to parts by weight to prepare composite enzyme for standby; mixing saccharomyces cerevisiae 12, lactobacillus plantarum 9, bacillus subtilis 18, candida utilis 70 and streptococcus lactis 19 according to parts by weight to prepare composite bacteria for standby;
(2) Mixing: according to the mass portion, the composite bacteria 51 and the composite enzyme 49 are mixed, and stirred for 5min under the conditions of the temperature of 20 ℃ and the stirring rotation speed of 60r/min, so as to obtain the starter.
Example 5: preparation of broussonetia papyrifera leaf chicken feed premix
(1) Crushing broussonetia papyrifera leaves: drying fresh folium Broussonetiae at 20deg.C for 9 hr, mechanically pulverizing, and sieving with 40 mesh sieve to obtain folium Broussonetiae powder;
(2) Preparing a paper mulberry culture medium: uniformly mixing the broussonetia papyrifera leaf powder 45, the corn meal 18, the wheat bran 3.2, the rice bran 6, the molasses 10 and the sodium chloride 0.3 according to mass fractions, and sterilizing for 20min at 121 ℃;
(3) Inoculating and fermenting: the mass ratio of the fermentation inoculant to the broussonetia papyrifera culture medium is 6:100 inoculating a fermentation inoculant (the fermentation inoculant prepared in the example 4) into a paper mulberry culture medium, and fermenting at 25 ℃ for 72 hours to obtain a premix with higher water content of the fermented paper mulberry;
(4) And (3) drying: drying the premix with higher water content of the fermented paper mulberry at 30 ℃ until the water content of the feed is lower than 5%, crushing, and sieving with a 50-mesh sieve to obtain the paper mulberry leaf chicken feed premix.
Example 6: preparation of broussonetia papyrifera leaf chicken feed premix
(1) Crushing broussonetia papyrifera leaves: drying fresh folium Broussonetiae at 25deg.C for 7 hr, mechanically pulverizing, and sieving with 40 mesh sieve to obtain folium Broussonetiae powder;
(2) Preparing a paper mulberry culture medium: mixing the broussonetia papyrifera leaf powder 50, the corn flour 20, the wheat bran 3.5, the rice bran 6.5, the molasses 12 and the sodium chloride 0.35 according to the parts by weight, and sterilizing for 20min at 121 ℃;
(3) Inoculating and fermenting: inoculating a fermentation inoculant into a paper mulberry culture medium, wherein the mass ratio of the fermentation inoculant (the fermentation inoculant prepared in the example 4) to the paper mulberry culture medium is 8:100, fermenting for 60 hours at the temperature of 30 ℃ to obtain a premix with higher water content of the fermented paper mulberry;
(4) And (3) drying: drying premix with higher water content of the fermented paper mulberry at 40 ℃ until the water content of the feed is lower than 5%, crushing, and sieving with a 50-mesh sieve to obtain the fermented paper mulberry leaf chicken feed premix.
Example 7: preparation of fermented broussonetia papyrifera leaf chicken feed premix
(1) Crushing broussonetia papyrifera leaves: drying fresh folium Broussonetiae at 30deg.C for 5 hr, mechanically pulverizing, and sieving with 40 mesh sieve to obtain folium Broussonetiae powder;
(2) Preparing a fermentation broussonetia papyrifera culture medium: mixing folium Broussonetiae powder 60, corn flour 18, testa Tritici 4, testa oryzae 6, molasses 11.7 and sodium chloride 0.3 according to weight parts, and sterilizing at 121deg.C for 20min;
(3) Inoculating and fermenting: the mass ratio of the fermentation inoculant to the broussonetia papyrifera culture medium is 11:100 inoculating a fermentation inoculant into a paper mulberry culture medium, and fermenting at 35 ℃ for 50 hours to obtain a premix with higher water content of the fermented paper mulberry;
(4) And (3) drying: drying premix with higher water content of the fermented paper mulberry at 40 ℃ until the water content of the feed is lower than 5%, crushing, and sieving with a 50-mesh sieve to obtain the fermented paper mulberry leaf chicken feed premix.
Example 8: nutrient composition change before and after fermentation of paper mulberry culture medium
The content of crude protein, crude fat and crude fiber in the broussonetia papyrifera feed premix prepared in example 7 was measured according to the methods of GB/T6432-1994, GB/T6433-2006 and GB/T6434-2006, the content of active peptide was measured according to the method of QB/T2653-2004, and the content of active polysaccharide was measured according to the phenol-sulfuric acid method. The measurement results are shown in Table 1.
TABLE 1 variation of nutrient composition before and after fermentation of Broussonetia papyrifera culture media
Note that: the different lowercase letters indicate that there is a significant difference (p < 0.05) between the nutritional ingredient indices before and after fermentation.
Table 1 shows that the crude protein content in the fermented Broussonetia papyrifera feed is increased from 19.5% to 23.9% before fermentation, by 22.6% and the difference is significant (p < 0.05). The content of peptide in the crude protein in the fermented paper mulberry feed is improved from 6.2% to 9.6% before fermentation, 54.8% is improved, and the difference is obvious (p is less than 0.05). The content of crude fat in the fermented paper mulberry feed is improved from 5.9% to 6.1% before fermentation, and is improved by 3.38%. The content of crude fiber in the fermented paper mulberry feed is reduced from 31.1% to 18.5% before fermentation, 40.5% is reduced, the difference is obvious (p is less than 0.05), the content of active polysaccharide in the fermented paper mulberry feed is increased from 3.5% to 7.3% before fermentation, the content is increased by 1.08 times, and the difference is obvious (p is less than 0.05). The method shows that the content of crude protein, peptide and active polysaccharide in the fermented paper mulberry feed is obviously increased after fermentation, crude fiber is further degraded, the content of the crude fiber is reduced, the digestion utilization rate of the feed is improved, and the nutritional value is further improved.
Example 9: influence of broussonetia papyrifera leaf chicken feed premix on growth performance of broilers
1. Test animals and test design
120 AA broilers were randomly divided into 2 groups of 60 chickens each with 4 replicates of 15 chickens each with single factor randomization. The control group was fed with basal diet and the test group was fed with basal diet containing 5% fermented broussonetia papyrifera leaf chicken feed premix (prepared as in example 7).
2. Feeding management and test records
Vaccine immunization and feeding management were performed according to AA broiler conventional procedure. Weighing on empty stomach of 1, 14, 28 and 42 days old respectively, and taking no water for 12 hours before weighing, and calculating daily average feed intake of AA broilers in each stage of 1-14 days old, 15-28 days old and 29-42 days old by using average daily gain in each stage and average daily gain in whole period.
The test results in table 2 show that the test results for feeding a feed premix containing 5% fermented broussonetia papyrifera leaf chickens are: compared with the control group, the daily average feed intake of the 1-14-day-old AA broiler is reduced by 2.92%, and the average daily gain is reduced by 2.97%. Compared with the control group, the daily average feed intake of the 15-28-day-old AA broiler is reduced by 0.9%, and the average daily gain is increased by 2.8%. Compared with the control group, the daily average feed intake of the 29-42-day-old AA broiler is reduced by 1.06%, the average daily gain is increased by 4.9%, and the difference is obvious (p is less than 0.05). From this, along with the perfect development of the digestion system of the broiler, the feed premix feed is gradually adapted to feed of the fermented broussonetia papyrifera leaf chicken feed, the daily average feed intake of 29-42 days old is reduced, the average daily gain is improved, and the utilization rate of the AA broiler chicken in the breeding stage for the fermented broussonetia papyrifera leaf chicken feed premix feed is obviously improved.
TABLE 2 Effect of fermented broussonetia papyrifera feed premix on broiler growth performance
Note that: different lower case letters indicate that there is a significant difference (p < 0.05) between the same stage groups.
The foregoing examples are merely illustrative of the preferred embodiments of the present invention and are not intended to limit the scope of the present invention, and various modifications and improvements made by those skilled in the art to which the present invention pertains should fall within the scope of the present invention defined by the appended claims.
Claims (10)
1. The fermenting bacteria agent is characterized by comprising compound bacteria and compound enzyme, wherein the mass ratio of the compound bacteria to the compound enzyme is (15-55): (25-60).
The composite bacteria comprise the following components in parts by weight: 10-20 parts of saccharomyces cerevisiae, 5-15 parts of lactobacillus plantarum, 10-25 parts of bacillus subtilis, 5-10 parts of candida utilis and 15-25 parts of streptococcus lactis;
the compound enzyme comprises the following components in parts by weight: 5-20 parts of cellulase, 10-15 parts of phytase, 5-15 parts of lignin enzyme and 1-8 parts of neutral protease.
2. The fermenting bacteria agent according to claim 1, wherein the saccharomyces cerevisiae is saccharomyces cerevisiae ATCC9080;
the lactobacillus plantarum is lactobacillus plantarum 22811;
the bacillus subtilis is bacillus subtilis CMCC (B) 63501;
the candida utilis is candida utilis ATCC22023;
the streptococcus lactis is streptococcus lactis ACCC11093.
3. The method for preparing the fermentation inoculant according to claim 1 or 2, comprising the following steps:
(1) Preparing materials: mixing 5-20 parts by weight of cellulase, 10-15 parts by weight of phytase, 5-15 parts by weight of lignin enzyme and 1-8 parts by weight of neutral protease to prepare a compound enzyme for standby;
mixing 10-20 parts of saccharomyces cerevisiae, 5-15 parts of lactobacillus plantarum, 10-25 parts of bacillus subtilis, 5-10 parts of candida utilis and 15-25 parts of streptococcus lactis according to parts by weight to prepare a composite bacterium for standby;
(2) Mixing: the composite bacteria and the composite enzyme are mixed according to the mass ratio of (15-55): (25-60) and stirring for 5min at 20 ℃ and stirring speed of 60r/min to obtain the fermentation inoculum.
4. The use of the fermenting bacteria agent in the production of broussonetia papyrifera feed premix according to claim 1.
5. The use according to claim 4, characterized by the steps of:
(1) Crushing broussonetia papyrifera leaves: drying fresh folium Broussonetiae at 20-30deg.C for 5-10h, mechanically pulverizing, and sieving with 50 mesh sieve to obtain folium Broussonetiae powder;
(2) Preparing a paper mulberry culture medium: uniformly mixing the broussonetia papyrifera leaf powder, corn flour, wheat bran, rice bran, molasses and sodium chloride, and sterilizing at 121 ℃ for 20min to obtain a broussonetia papyrifera culture medium;
(3) Inoculating and fermenting: inoculating the fermentation inoculant of claim 1 into a paper mulberry culture medium, and fermenting to obtain a premix;
(4) And (3) drying: drying the premix, pulverizing, and sieving to obtain the folium Broussonetiae chicken feed premix.
6. The use according to claim 5, wherein in step (2), the mass ratio of the paper mulberry leaf powder, corn flour, wheat bran, rice bran, molasses, sodium chloride is (45-65): (10-25): (3-5): (5-7): (5-15): (0.2-0.4).
7. The use according to claim 5, wherein in step (3), the mass ratio of the fermentation broth to the broussonetia papyrifera medium is (5-15): 100.
8. the method according to claim 5, wherein in step (3), the fermentation is performed at a temperature of 25 to 35 ℃ for a period of 48 to 84 hours.
9. The use according to claim 5, wherein in step (4), the drying is performed at 30-50 ℃, and the mass content of water in the premix after drying is less than 5%.
10. The use according to claim 5, wherein in step (4), the sieving is by a 40-60 mesh sieve.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202311758633.4A CN117757648A (en) | 2023-12-20 | 2023-12-20 | Fermentation inoculant and application thereof in production of broussonetia papyrifera feed premix |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202311758633.4A CN117757648A (en) | 2023-12-20 | 2023-12-20 | Fermentation inoculant and application thereof in production of broussonetia papyrifera feed premix |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN117757648A true CN117757648A (en) | 2024-03-26 |
Family
ID=90309874
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202311758633.4A Pending CN117757648A (en) | 2023-12-20 | 2023-12-20 | Fermentation inoculant and application thereof in production of broussonetia papyrifera feed premix |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN117757648A (en) |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106942143A (en) * | 2017-03-16 | 2017-07-14 | 南丹县三丰绿色生态农牧开发有限责任公司（微型企业） | A kind of method that utilization feed with paper-mulberry leaf raises Heptacoloured pheasant |
| CN107568415A (en) * | 2017-10-24 | 2018-01-12 | 山西大禹生物工程股份有限公司 | A kind of livestock and poultry biological fermentation feed |
| KR20180051023A (en) * | 2016-11-08 | 2018-05-16 | 김혜미 | Manufacturing method of probiotics for feed additives and probiotics manufactured by the method |
| CN110973355A (en) * | 2019-12-05 | 2020-04-10 | 安徽宝楮生态农业科技有限公司 | Whole-plant hybrid broussonetia papyrifera biological feed leavening agent |
| CN112175846A (en) * | 2020-10-22 | 2021-01-05 | 河南省科学院生物研究所有限责任公司 | Candida utilis strain UCY-11 and application thereof in preparation of fermented hybrid broussonetia papyrifera feed |
| CN112544787A (en) * | 2020-11-02 | 2021-03-26 | 浙江大学 | Method for fermenting paper mulberry compound feed by bacteria and enzyme in synergy mode and compound feed thereof |
| CN112640993A (en) * | 2019-10-11 | 2021-04-13 | 博益德（北京）生物科技有限公司 | Fermented feed containing hybrid paper mulberry, and preparation method and application thereof |
-
2023
- 2023-12-20 CN CN202311758633.4A patent/CN117757648A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20180051023A (en) * | 2016-11-08 | 2018-05-16 | 김혜미 | Manufacturing method of probiotics for feed additives and probiotics manufactured by the method |
| CN106942143A (en) * | 2017-03-16 | 2017-07-14 | 南丹县三丰绿色生态农牧开发有限责任公司（微型企业） | A kind of method that utilization feed with paper-mulberry leaf raises Heptacoloured pheasant |
| CN107568415A (en) * | 2017-10-24 | 2018-01-12 | 山西大禹生物工程股份有限公司 | A kind of livestock and poultry biological fermentation feed |
| CN112640993A (en) * | 2019-10-11 | 2021-04-13 | 博益德（北京）生物科技有限公司 | Fermented feed containing hybrid paper mulberry, and preparation method and application thereof |
| CN110973355A (en) * | 2019-12-05 | 2020-04-10 | 安徽宝楮生态农业科技有限公司 | Whole-plant hybrid broussonetia papyrifera biological feed leavening agent |
| CN112175846A (en) * | 2020-10-22 | 2021-01-05 | 河南省科学院生物研究所有限责任公司 | Candida utilis strain UCY-11 and application thereof in preparation of fermented hybrid broussonetia papyrifera feed |
| CN112544787A (en) * | 2020-11-02 | 2021-03-26 | 浙江大学 | Method for fermenting paper mulberry compound feed by bacteria and enzyme in synergy mode and compound feed thereof |
Non-Patent Citations (2)
| Title |
|---|
| 张秀江等: "复合菌固体发酵构树生产蛋白饲料的试验研究", 《饲料工业》, vol. 41, no. 4, 31 December 2020 (2020-12-31), pages 34 - 39 * |
| 王 洋等: "不同菌制剂对全株构树青贮饲料品质的影响", 《饲料研究》, no. 6, 31 December 2021 (2021-12-31), pages 105 - 108 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN111436526A (en) | Preparation method and application of fermented rice bran meal with bacterium enzyme for improving growth performance of fattening pigs | |
| CN101120719B (en) | Microorganism ferment purple sweet potato and preparation method thereof | |
| CN103667220B (en) | Neutral protease-containing growing pig dedicated enzyme and preparation method thereof | |
| CN111264698B (en) | Mixed-strain fermented feed additive for treating piglet diarrhea and preparation method and application thereof | |
| CN110338278A (en) | One main laminaria fermented feed containing probiotics and preparation method thereof | |
| CN110583855A (en) | Preparation method of high tryptophan fermented feed | |
| CN111296723A (en) | Probiotic agent for multi-strain combined solid state fermentation of rapeseed meal and fermentation method thereof | |
| CN105494919A (en) | Yeast culture fermented by mixed bacteria and preparation method of yeast culture | |
| CN111296679B (en) | Fermented feed for laying hens and preparation method thereof | |
| CN111248354A (en) | Sheep feed based on fermented bamboo shoot shells and preparation method thereof | |
| CN116391796A (en) | Method for processing wangcao | |
| CN116889259A (en) | Method for improving nutritive value of high Wen Shou hot pickled mustard tuber seed meal and application | |
| CN115137012B (en) | Mulberry leaf source protein compound feed based on multiple fermentation of composite flora and application thereof | |
| CN111280307A (en) | Additive for soybean meal fermentation and application of additive in feed | |
| CN116058432A (en) | Citric acid mycelium residue microbial feed additive and preparation method and application thereof | |
| CN117757648A (en) | Fermentation inoculant and application thereof in production of broussonetia papyrifera feed premix | |
| CN103740683B (en) | A kind of Wheat ration enzyme containing neutral protease and preparation method thereof | |
| CN112772770A (en) | Preparation method of stevia rebaudiana fermentation product and milch goat feed | |
| CN107897505B (en) | Zymophyte liquid, product containing zymophyte liquid and used for nursing pigs, and preparation method and application of product | |
| CN111700176A (en) | Processing method of biological feed for fattening pigs | |
| CN112586615A (en) | Preparation method of beef cattle feed | |
| CN100341421C (en) | Method for producing biological protein drink by king grass | |
| CN110200159A (en) | Live pig fermented feed and preparation method thereof | |
| CN116784418B (en) | Plant-derived protein compound feed based on complex flora multiple fermentation | |
| CN117821298A (en) | Broussonetia papyrifera leaf fermentation inoculant and application thereof in production of pig feed premix |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |