CN117752776B - Pharmaceutical preparation and application thereof in preparation of drugs for treating stress injury of Babylonia after transportation - Google Patents
Pharmaceutical preparation and application thereof in preparation of drugs for treating stress injury of Babylonia after transportation Download PDFInfo
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- CN117752776B CN117752776B CN202410194760.4A CN202410194760A CN117752776B CN 117752776 B CN117752776 B CN 117752776B CN 202410194760 A CN202410194760 A CN 202410194760A CN 117752776 B CN117752776 B CN 117752776B
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- 239000000825 pharmaceutical preparation Substances 0.000 title claims abstract description 18
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- YMAWOPBAYDPSLA-UHFFFAOYSA-N glycylglycine Chemical compound [NH3+]CC(=O)NCC([O-])=O YMAWOPBAYDPSLA-UHFFFAOYSA-N 0.000 claims abstract description 30
- 239000008194 pharmaceutical composition Substances 0.000 claims abstract description 23
- AYIRNRDRBQJXIF-NXEZZACHSA-N (-)-Florfenicol Chemical compound CS(=O)(=O)C1=CC=C([C@@H](O)[C@@H](CF)NC(=O)C(Cl)Cl)C=C1 AYIRNRDRBQJXIF-NXEZZACHSA-N 0.000 claims abstract description 17
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Landscapes
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
The invention discloses a pharmaceutical preparation. The pharmaceutical formulation comprises: agent A and agent B; the agent A comprises the following components in parts by weight: 8-15 parts of florfenicol and 6-14 parts of sulfamethoxazole; the agent B comprises the following components: 10-20 parts of allicin, 5-8 parts of glucose oxidase, 2-4 parts of beta-sitosterol and 1-3 parts of glycylglycine. In addition, the invention also claims the application of the pharmaceutical preparation in preparing the medicine for treating the stress injury after transportation of the Babylonia. The pharmaceutical preparation provided by the invention adopts the agent A and the agent B to be matched for use, can play a role in synergistically improving the drug effect, has excellent treatment effect and blocking effect on the stress injury of the Babylonia after transportation, and can obviously reduce the incidence rate of the stress injury of the Babylonia.
Description
Technical Field
The invention belongs to the technical field of snail breeding, and in particular relates to a pharmaceutical preparation and application thereof in preparing a medicament for treating stress injury of Babylonia after transportation.
Background
The Babylonia, the aliases of Babylonia, sweet snail, southern snail, yellow snail and flower snail belong to Mollusca (Mollusca), gastropoda (Gastropoda), front gill subclass (Prosobranchia), celloda (Neogastropoda), moth snail (Buccinidae) and Babylonia (Babylonia) and are main cultured shellfish varieties in China. The Babylonia snail meat is fine and smooth, delicious in taste and has the reputation of 'pearl in dish'; it is rich in protein, vitamins, amino acids and trace elements essential to human body, and is a typical natural animal health food with high protein, low fat and high calcium.
The eastern conch breeding or propagation often involves transportation, but eastern conch is easy to generate stress reaction in the transportation process, so that resistance is reduced, the health condition is poor, and the eastern conch cannot be well adapted to a new environment, and disease death is often induced. In addition, the Babylonia is extremely easy to stab the feet of each other and cause violent struggling because of the problems of strong stress, sharp tail, thin shell and the like in the transportation process, so that mechanical damage is caused, and the Babylonia is more difficult to adapt to new environments due to high transportation density and jolting in the way. The mechanical damage can aggravate the stress response of the Babylonia in the transportation process, and further the death of the Babylonia is more easily caused.
Patent CN113812357A discloses a keep-alive transportation method for slowing down oxidation stress damage of sea bass, wherein emulsified herba Melissae axillaris essential oil is added into a transportation water body of a PE bag, the water temperature is controlled at 12 (+ -1) DEGC, the concentration of the herba Melissae axillaris essential oil is respectively 10 mg/L, 20 mg/L and 40mg/L, and the sea bass is kept alive and transported within the concentration range, so that the energy consumption and metabolism rate of the sea bass in the process of keeping-alive and transportation can be reduced; slowing down damage and apoptosis induced by keep-alive transport to gill, liver and kidney tissues; delay the rise of serum cortisol, stress protein, lactic acid, blood sugar and oxidase activity of sea bass; enhancing the total antioxidant capacity of sea bass during transportation. However, the method is difficult to be applied to the cultivation of other aquatic animals, and mechanical external damage is less generated in the sea bass transportation process. Therefore, how to develop more schemes capable of relieving stress injury and suitable for the stress injury after transportation of Babylonia is a technical problem to be solved.
Disclosure of Invention
Aiming at the prior art problems, the primary aim of the invention is to provide a pharmaceutical preparation, wherein the pharmaceutical preparation is prepared by matching an agent A and an agent B, has excellent treatment effect and blocking effect on the stress injury of the Babylonia after transportation, and can remarkably reduce the incidence rate of the stress injury of the Babylonia.
A second object of the present invention is to provide a process for the preparation of a pharmaceutical formulation.
The third object of the invention is to provide an application of a pharmaceutical preparation in preparing a medicament for treating stress injury after transportation of Babylonia.
The fourth object of the invention is to provide a cultivation method of the Babylonia after transportation.
In order to achieve the above object, the present invention is realized by the following technical scheme:
A pharmaceutical formulation comprising: agent A and agent B; the agent A comprises the following components in parts by weight: 8-15 parts of florfenicol and 6-14 parts of sulfamethoxazole; the agent B comprises the following components: 10-20 parts of allicin, 5-8 parts of glucose oxidase, 2-4 parts of beta-sitosterol and 1-3 parts of glycylglycine.
According to the invention, after the Babylonia is transported, the drug preparation A containing florfenicol and sulfamethoxazole and the drug preparation B containing allicin, glucose oxidase, beta-sitosterol and glycylglycine are sequentially added, so that the agent A has a killing or certain inhibition effect on pathogenic bacteria; the agent B can simulate natural culture environment, is suitable for Babylonia, helps the Babylonia resist urgent reaction caused by environmental change, maintains organism activity, and reduces stress. Under the synergistic effect of the components, the Chinese medicinal composition can improve the curative effect, effectively inhibit bacteria, repair intestinal tracts, eliminate inflammation, reduce stress injury and improve the immunity function of the Babylonia body. The compound preparation has excellent treatment effect and blocking effect on the stress injury of the Babylonia after transportation, can obviously reduce the incidence rate of the stress injury of the Babylonia, can control the problem of the stress injury of the Babylonia and is not easy to relapse.
Preferably, the mass ratio of the agent A to the agent B is 2-5: 2.5 to 4. Further preferably, the mass ratio of the agent A to the agent B is 3.5-4.5: 3 to 4.
Preferably, the agent A comprises the following components in parts by weight: 12-15 parts of florfenicol and 10-12 parts of sulfamethoxazole; the agent B comprises the following components: 18-20 parts of allicin, 6-8 parts of glucose oxidase, 3-4 parts of beta-sitosterol and 2-3 parts of glycylglycine.
Furthermore, the invention claims a preparation method of a pharmaceutical preparation, which comprises the steps of uniformly mixing florfenicol and sulfamethoxazole to prepare an agent A; and uniformly mixing allicin, glucose oxidase, beta-sitosterol and glycylglycine to prepare the agent B.
Further, the invention claims the application of a pharmaceutical preparation in preparing a medicament for treating stress injury after transportation of Babylonia.
In the invention, the stress injury refers to that the Babylonia is not suitable for a new environment, and any one or more symptoms such as the weakness of the expansion of the gastropod or incapability of retracting into the shell, the swelling of the gastropod caused by water absorption, the red swelling of the anastomat tube, the upward gastropod or the recumbent spiral body, no ingestion or slow ingestion and the like appear successively, and finally death is caused.
Further, the invention claims a cultivation method of transported Babylonia, wherein the transported Babylonia is soaked by using the agent A, water is changed, and then the Babylonia is soaked by using the agent B; the agent A comprises the following components in parts by weight: 8-15 parts of florfenicol and 6-14 parts of sulfamethoxazole; the agent B comprises the following components: 10-20 parts of allicin, 5-8 parts of glucose oxidase, 2-4 parts of beta-sitosterol and 1-3 parts of glycylglycine.
Preferably, the soaking time of the agent A is 2-3 hours.
Preferably, the soaking time of the agent B is 1-2 hours.
Preferably, when the agent A and the agent B are applied, the water level of the pool is reduced to 10-15 cm away from the sand surface. At this level, oxygen can reach the level of the water that is pumped out. The waste of the medicine can be reduced by lowering the water level, and the oxygen can reach the degree of pumping out water, so that the oxygen deficiency caused by long-time oxygen stopping can be prevented; in addition, the medicine can be uniform, and the sediment is prevented from being deposited on the sand layer.
Preferably, the agent A and the agent B are used continuously for 3 to 4 days.
Specifically, the Babylonia is Babylonia genus (Babylonia) including, but not limited to, babylonia (Babylonia areolata), babylonia (lutosa), babylonia (Babylonia formosae), and the like.
Compared with the prior art, the invention has the following beneficial effects:
(1) The invention provides a pharmaceutical preparation, wherein an agent A and an agent B are adopted for matching, and the agent A has a killing or a certain inhibiting effect on pathogenic bacteria; the agent B can simulate a natural culture environment, is suitable for the Babylonia, helps the Babylonia resist urgent reaction caused by environmental change, maintains organism activity, and reduces stress; the invention can play a role in synergistically improving the drug effect by matching the agent A and the agent B, has excellent treatment effect on the stress injury of the Babylonia after transportation, and remarkably reduces the incidence rate of the stress injury of the Babylonia after transportation.
(2) The pharmaceutical preparation provided by the invention is green and safe, and has no toxicity, harm, side effect and adverse reaction to Babylonia.
Drawings
FIG. 1 is a schematic diagram of packaging and packing Babylonia, a Zhanjiang mud, into a foam box just captured from a culture pond.
Fig. 2 is a state diagram of the Babylonia which is a transport mud from Zhanjiang to Hainan and is put into a culture pond after being subjected to stress injury.
Fig. 3 is a diagram of dead Babylonia with stress injury.
Fig. 4 is a schematic field diagram of a treatment trial group of eastern whelk stress injury.
Fig. 5 is a state diagram of the first day of Babylonia after bath treatment in example 5.
FIG. 6 is a state diagram of Babylonia on the next day after bath treatment with the traditional Chinese medicine of example 5.
FIG. 7 is a state diagram of Babylonia on the third day after bath treatment in example 5.
Detailed Description
The invention is further illustrated in the following drawings and specific examples, which are not intended to limit the invention in any way. Unless specifically stated otherwise, the reagents, methods and apparatus employed in the present invention are those conventional in the art.
Example 1A pharmaceutical formulation
A pharmaceutical formulation comprising an agent a and an agent B; the preparation method of the agent A comprises the following steps: and (3) uniformly mixing 12 parts of florfenicol and 10 parts of sulfamethoxazole according to parts by weight to obtain the agent A. The preparation method of the agent B comprises the following steps: and uniformly mixing 20 parts of allicin, 6 parts of glucose oxidase, 4 parts of beta-sitosterol and 2 parts of glycylglycine according to parts by weight to obtain the agent B.
EXAMPLE 2A pharmaceutical formulation
A pharmaceutical formulation comprising an agent a and an agent B; the preparation method of the agent A comprises the following steps: and (3) uniformly mixing 15 parts of florfenicol and 12 parts of sulfamethoxazole according to parts by weight to obtain the agent A. The preparation method of the agent B comprises the following steps: according to parts by weight, 18 parts of allicin, 8 parts of glucose oxidase, 3 parts of beta-sitosterol and 3 parts of glycylglycine are uniformly mixed to obtain the agent B.
EXAMPLE 3A pharmaceutical formulation
A pharmaceutical formulation comprising an agent a and an agent B; the preparation method of the agent A comprises the following steps: and (3) uniformly mixing 8 parts of florfenicol and 14 parts of sulfamethoxazole according to parts by weight to obtain the agent A. The preparation method of the agent B comprises the following steps: and uniformly mixing 10 parts of allicin, 8 parts of glucose oxidase, 4 parts of beta-sitosterol and 1 part of glycylglycine according to parts by weight to obtain the agent B.
EXAMPLE 4A pharmaceutical formulation
A pharmaceutical formulation comprising an agent a and an agent B; the preparation method of the agent A comprises the following steps: and (3) uniformly mixing 12 parts of florfenicol and 6 parts of sulfamethoxazole according to parts by weight to obtain the agent A. The preparation method of the agent B comprises the following steps: and (3) uniformly mixing 15 parts of allicin, 5 parts of glucose oxidase, 2 parts of beta-sitosterol and 2 parts of glycylglycine according to parts by weight to obtain the agent B.
EXAMPLE 5 therapeutic test of pharmaceutical formulations against Tonic stress injury
From Zhanjiang transportation of 128 jin of mud Babylonia to Hainan, the mud Babylonia reaches the Hainan with the specification of 35-40 g (shown in figure 1) and has the phenomena of stress injury and large-scale death of different degrees. Stress injury is manifested as: the eastern wind snail sequentially has symptoms of weak expansion of the gastropod or incapability of retracting into the shell, swelling of the gastropod due to water absorption, red and swollen kistrodon, upward gastropod or recumbent spiral body, no ingestion or slow ingestion, and the like, and finally causes death; in addition, some of the soft parts of the Babylonia are separated from the shells and ingested by other Babylonia to infect them, leaving empty shells and small spots with gastropod muscles (as shown in FIGS. 2 and 3).
And fishing out the mud Babylonia with stress injury, discarding, incinerating to prevent infection, and sterilizing the whole pond by povidone iodine. And (3) carrying out a grouping medicine test on mud snails which do not show symptoms (as shown in figure 4), fishing out 100 mud snails, placing the mud snails in a white test box, and paving fine sand with the diameter of 1-3 mm in the box for 2 hours to flow water. The mud Babylonia is soaked in a medicated bath by adopting the pharmaceutical preparation prepared in the embodiment 1, and the specific operation is as follows:
Firstly, the water supply valve of the water pipe of the test box is closed, the water adding is stopped, the drainer is opened, and the height of the water in the test box is reduced to a position where the bubble stone can throw out oxygen in a vertical state or the depth of 3-5 cm is suitable for submerging the spiral body of the Babylonia. In the morning, 40ppm (based on the volume of water in the test chamber) of the agent A prepared in example 1 was dissolved in clean seawater, and poured into the test chamber to soak Babylonia in the agent A, and after 3 hours, the water supply valve was opened to change water. The water level is reduced in the afternoon according to the operation method, 35ppm (based on the volume of water in the test box) of the agent B prepared in the example 1 is poured into the test box, so that the Babylonia is soaked in the agent B, the whole body of the screw body is bathed by the liquid medicine, the medicated bath is carried out for 2 hours, water is supplied and drained at the same time, and the water environment in the test box is kept clean.
The treatment period was 3 days (as shown in fig. 5, 6 and 7), and each of the doses a and B was administered once daily. The tours are patrolled for 3 times in the morning, in the middle and at the evening, and the eastern conch with stress injury symptoms are timely picked up and recorded. After one cycle of treatment, observations were continued for 4 months, and eastern whelk was no longer ill.
EXAMPLE 6 therapeutic test of pharmaceutical formulations against Tonic stress injury
The difference between this embodiment and embodiment 5 is that: the pharmaceutical formulation of example 2 was used.
EXAMPLE 7 therapeutic test of pharmaceutical formulations against Tonic stress injury
The difference between this embodiment and embodiment 5 is that: 50ppm of the pharmaceutical formulation A prepared in example 3 was used; 25ppm of pharmaceutical formulation B prepared in example 3 was used.
EXAMPLE 8 therapeutic test of pharmaceutical formulations against Tonic stress injury
The difference between this embodiment and embodiment 5 is that: 20ppm of the pharmaceutical formulation A prepared in example 4 was used; 40ppm of pharmaceutical formulation B prepared in example 4 was used.
Comparative example 1
The difference between this comparative example and example 5 is that: the pharmaceutical formulation of example 1 was not used in this comparative example.
Comparative example 2
The difference between this comparative example and example 5 is that: florfenicol was used in place of the agent A prepared in example 1.
Comparative example 3
The difference between this comparative example and example 5 is that: sulfamethoxazole was used in place of the agent A prepared in example 1.
Comparative example 4
The difference between this comparative example and example 5 is that: the agent B contains no allicin.
Comparative example 5
The difference between this comparative example and example 5 is that: the agent B contains no glucose oxidase.
Comparative example 6
The difference between this comparative example and example 5 is that: the agent B does not contain beta-sitosterol.
Comparative example 7
The difference between this comparative example and example 5 is that: the agent B does not contain glycylglycine.
Comparative example 8
The difference between this comparative example and example 5 is that: the agent A prepared in example 1 was replaced with amoxicillin soluble powder (source: guangzhou gold aquatic animal health products Co., ltd., trade name "Junpudi").
Comparative example 9
The difference between this comparative example and example 5 is that: the preparation A prepared in example 1 was replaced with neomycin sulfate powder (source: guangzhou gold aquatic animal health products Co., ltd., trade name "Chang Gankang").
Comparative example 10
The difference between this comparative example and example 5 is that: no agent a was used in this comparative example.
Comparative example 11
The difference between this comparative example and example 5 is that: no agent B was used in this comparative example.
Test example 1
The incidence of stress injury of Babylonia in examples 5 to 6 and comparative examples 1 to 11 was tested as follows:
Incidence of stress injury: the number of developed eastern conch within 3 days was compared to the total number of eastern conch in the test chamber.
Calculation formula of test data: incidence = number of screws/total number of screws in 3 days x 100%.
Judgment criteria for onset of stress injury: the phenomena of empty shell, telescopic and weak gastropod or incapability of retracting into the shell, swelling of the water absorption of the gastropod, red swelling of the anastomat tube, hardening of the gastropod, upward gastropod, turning over of a screw body, recumbent and the like are symptoms of stress injury.
The number of Babylonia in each test box was 100, and the test results of each set of examples and comparative examples are shown in Table 1.
TABLE 1
From the above examples 5 to 8, it is known that the pharmaceutical preparation provided by the invention has excellent therapeutic effect and blocking effect on the stress injury of the transported Babylonia after the transportation of the Babylonia by carrying out medicated bath treatment on the transported Babylonia, and can remarkably reduce the incidence of the stress injury of the Babylonia, and the incidence of the stress injury of the Babylonia is less than or equal to 7%.
From example 5 and comparative example 1, it is understood that the incidence of stress injury after transportation of Babylonia is as high as 95% without any measure.
From example 5, comparative example 2 and comparative example 3, it is understood that when the combination of florfenicol and sulfamethoxazole is adopted in the pharmaceutical preparation, the composition has excellent therapeutic effect and blocking effect on stress injury after transportation of Babylonia, and the incidence of stress injury of Babylonia can be remarkably reduced.
As is clear from examples 5 and comparative examples 4 to 7, it is difficult to achieve the technical effects of the present invention when a B-agent lacking any one of allicin, glucose oxidase, β -sitosterol and diglycerin is used.
From example 5, comparative example 8 and comparative example 9, it is known that florfenicol and sulfamethoxazole have excellent therapeutic effects on stress injury after transportation of Babylonia. While some conventional antimicrobial agents in the art have little therapeutic effect on the stress injury of Babylonia.
As is clear from examples 5, 10 and 11, the combination of the agent a and the agent B can synergistically enhance the efficacy of the drug, and the drug preparation provided by the invention has excellent therapeutic effect and blocking effect on stress injury after transportation of Babylonia.
The foregoing examples are illustrative only and serve to explain some features of the method of the invention. The claims that follow are intended to claim the broadest possible scope as conceivable and the embodiments presented herein are demonstrated for the applicant's true test results. It is, therefore, not the intention of the applicant that the appended claims be limited by the choice of examples illustrating the features of the invention. Some numerical ranges used in the claims also include sub-ranges within which variations in these ranges should also be construed as being covered by the appended claims where possible.
Claims (9)
1. A pharmaceutical preparation composition is characterized by comprising an agent A and an agent B which are matched for use; the A agent consists of 8-15 parts by weight of florfenicol and 6-14 parts by weight of sulfamethoxazole; the agent B consists of 10-20 parts of allicin, 5-8 parts of glucose oxidase, 2-4 parts of beta-sitosterol and 1-3 parts of glycylglycine; the mass ratio of the agent A to the agent B is 2-5: 2.5 to 4.
2. The pharmaceutical preparation composition according to claim 1, wherein the agent a consists of 12-15 parts by weight of florfenicol and 10-12 parts by weight of sulfamethoxazole; the agent B consists of 18-20 parts of allicin, 6-8 parts of glucose oxidase, 3-4 parts of beta-sitosterol and 2-3 parts of glycylglycine.
3. A process for preparing a pharmaceutical formulation composition according to claim 1 or 2, comprising the steps of: uniformly mixing florfenicol and sulfamethoxazole to prepare a preparation A; and uniformly mixing allicin, glucose oxidase, beta-sitosterol and glycylglycine to prepare the agent B.
4. Use of a pharmaceutical formulation composition according to claim 1 or 2 for the manufacture of a medicament for the prevention of stress injury after transportation of Babylonia.
5. A method for culturing transported Babylonia for the purpose of non-disease treatment, comprising the following steps: soaking transported Babylonia by using an agent A, changing water after soaking, and then soaking by using an agent B;
The A agent consists of 8-15 parts by weight of florfenicol and 6-14 parts by weight of sulfamethoxazole; the agent B consists of 10-20 parts of allicin, 5-8 parts of glucose oxidase, 2-4 parts of beta-sitosterol and 1-3 parts of glycylglycine; the mass ratio of the agent A to the agent B is 2-5: 2.5 to 4.
6. The method according to claim 5, wherein the time for immersing the agent A is 2 to 3 hours.
7. The method according to claim 5, wherein the time for immersing the agent B is 1 to 2 hours.
8. The culture method according to claim 5, wherein the pool water level is lowered to 10 to 15cm from the sand surface when the agent A and the agent B are used.
9. The culture method according to claim 5, wherein the agent A and the agent B are used continuously for 3 to 4 days.
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