CN1177046C - 增强的病毒介导的dna转移 - Google Patents
增强的病毒介导的dna转移Info
- Publication number
- CN1177046C CN1177046C CNB951932683A CN95193268A CN1177046C CN 1177046 C CN1177046 C CN 1177046C CN B951932683 A CNB951932683 A CN B951932683A CN 95193268 A CN95193268 A CN 95193268A CN 1177046 C CN1177046 C CN 1177046C
- Authority
- CN
- China
- Prior art keywords
- thr
- pro
- cell
- val
- leu
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
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- C07K14/78—Connective tissue peptides, e.g. collagen, elastin, laminin, fibronectin, vitronectin or cold insoluble globulin [CIG]
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- C—CHEMISTRY; METALLURGY
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- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
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- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2217/00—Genetically modified animals
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- C12N2740/13043—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
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- C12N2740/00—Reverse transcribing RNA viruses
- C12N2740/00011—Details
- C12N2740/10011—Retroviridae
- C12N2740/16011—Human Immunodeficiency Virus, HIV
- C12N2740/16041—Use of virus, viral particle or viral elements as a vector
- C12N2740/16043—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
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Abstract
Description
实验 | 牛血清白蛋白(BSA) | 30/35 FN |
实验1 | 1/18* | 13/14 |
实验2 | 2/13 | 12/13 |
实验 | BSA | 30/35FN |
实验1 | 0/14* | 10/19 |
实验2 | N/A | 2/12 |
实验3 | 0/4 | 3/5 |
实验4 | 0/4 | 6/6 |
总计 | 0/22 | 21/42 |
BSA 12±17 |
30/35FN 55±16 |
载体 | 产物/克隆 | 表达的cDNA |
PGK-hADA | GP+E86/EPHA-5 | 人ADA |
PM5neo | GP+E86/EAL2a | neo磷酸转移酶LAC-Z |
TKNeo | GP+E86/TKNeo | neo磷酸转移酶 |
PGK-mADA | GP+EnvAM 12/55/6 | 鼠ADA |
Claims (65)
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US08/218,355 | 1994-03-25 | ||
US08/218,355 US5686278A (en) | 1994-03-25 | 1994-03-25 | Methods for enhanced retrovirus-mediated gene transfer |
Related Child Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN200410082654XA Division CN1775946B (zh) | 1994-03-25 | 1995-03-27 | 增强的病毒介导的dna转移 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN1152875A CN1152875A (zh) | 1997-06-25 |
CN1177046C true CN1177046C (zh) | 2004-11-24 |
Family
ID=22814775
Family Applications (3)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CNB2004100905683A Expired - Lifetime CN100567488C (zh) | 1994-03-25 | 1995-03-27 | 增强的病毒介导的dna转移 |
CNB951932683A Expired - Lifetime CN1177046C (zh) | 1994-03-25 | 1995-03-27 | 增强的病毒介导的dna转移 |
CN200410082654XA Expired - Lifetime CN1775946B (zh) | 1994-03-25 | 1995-03-27 | 增强的病毒介导的dna转移 |
Family Applications Before (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CNB2004100905683A Expired - Lifetime CN100567488C (zh) | 1994-03-25 | 1995-03-27 | 增强的病毒介导的dna转移 |
Family Applications After (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN200410082654XA Expired - Lifetime CN1775946B (zh) | 1994-03-25 | 1995-03-27 | 增强的病毒介导的dna转移 |
Country Status (13)
Country | Link |
---|---|
US (1) | US5686278A (zh) |
EP (2) | EP0752874B1 (zh) |
JP (4) | JP3411039B2 (zh) |
KR (2) | KR100445712B1 (zh) |
CN (3) | CN100567488C (zh) |
AT (1) | ATE370229T1 (zh) |
AU (1) | AU690667B2 (zh) |
CA (1) | CA2185712C (zh) |
DE (1) | DE69535560T2 (zh) |
ES (1) | ES2294781T3 (zh) |
RU (1) | RU2174846C2 (zh) |
TW (1) | TWI232884B (zh) |
WO (1) | WO1995026200A1 (zh) |
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US7083979B1 (en) * | 1994-03-25 | 2006-08-01 | Indiana University Foundation | Methods for enhanced retroviral-mediated gene transfer |
ATE325533T1 (de) * | 1995-09-29 | 2006-06-15 | Univ Indiana Res & Tech Corp | Verfahren zum verbesserten virusvermittelten dns- transfer unter verwendung von molekülen mit virus-und zellbindenden domänen |
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EP0930361B1 (en) * | 1996-07-10 | 2003-06-11 | Takara Bio Inc. | Cell compositions |
CA2283641A1 (en) * | 1997-03-18 | 1998-09-24 | Introgene B.V. | Methods and compositions for genetically modifying primate bone marrow cells |
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ATE507240T1 (de) * | 2004-03-05 | 2011-05-15 | Vegenics Pty Ltd | Materialien und verfahren für wachstumsfaktorbindende konstrukte |
US7319015B2 (en) * | 2004-03-16 | 2008-01-15 | The Regents Of The University Of Michigan | Methods and compositions for using alveolar macrophage phospholipase A2 |
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FI20050753A (fi) | 2004-09-03 | 2006-03-04 | Licentia Oy | Uudet peptidit |
US20070275874A1 (en) * | 2004-09-03 | 2007-11-29 | Yale University | Use of Leptin in Wound Healing |
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JPWO2006082958A1 (ja) | 2005-02-07 | 2008-06-26 | タカラバイオ株式会社 | レトロウイルスの保存方法 |
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JP4860612B2 (ja) | 2005-06-15 | 2012-01-25 | タカラバイオ株式会社 | 脂肪細胞あるいは前駆脂肪細胞への遺伝子導入方法 |
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US8052970B2 (en) * | 2008-06-30 | 2011-11-08 | The Regents Of The University Of Michigan | Lysosomal phospholipase A2 (LPLA2) activity as a diagnostic and therapeutic target for identifying and treating systemic lupus erythematosis |
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US5198423A (en) * | 1989-05-26 | 1993-03-30 | Takara Shuzo Co., Ltd. | Functional polypeptide containing a cell binding domain and a heparin binding domain of fibronectin |
CA2137558A1 (en) * | 1992-07-17 | 1994-02-03 | Wayne A. Marasco | Method of intracellular binding of target molecules |
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JP2003111598A (ja) | 2003-04-15 |
EP0752874A1 (en) | 1997-01-15 |
CN1152875A (zh) | 1997-06-25 |
KR100506570B1 (ko) | 2005-08-10 |
JPH09510874A (ja) | 1997-11-04 |
EP0752874B1 (en) | 2007-08-15 |
DE69535560T2 (de) | 2008-04-30 |
ES2294781T3 (es) | 2008-04-01 |
EP0752874A4 (en) | 1999-05-12 |
AU690667B2 (en) | 1998-04-30 |
WO1995026200A1 (en) | 1995-10-05 |
CN1775946A (zh) | 2006-05-24 |
CN1775946B (zh) | 2010-11-10 |
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CN1632116A (zh) | 2005-06-29 |
CN100567488C (zh) | 2009-12-09 |
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CA2185712C (en) | 2002-10-22 |
US5686278A (en) | 1997-11-11 |
DE69535560D1 (de) | 2007-09-27 |
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