CN117547561B - 一种具有促进消化和吸收功能的生物药品 - Google Patents
一种具有促进消化和吸收功能的生物药品 Download PDFInfo
- Publication number
- CN117547561B CN117547561B CN202311509496.0A CN202311509496A CN117547561B CN 117547561 B CN117547561 B CN 117547561B CN 202311509496 A CN202311509496 A CN 202311509496A CN 117547561 B CN117547561 B CN 117547561B
- Authority
- CN
- China
- Prior art keywords
- curdlan
- fermentation
- parts
- digestion
- lactobacillus
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 230000029087 digestion Effects 0.000 title claims abstract description 32
- 239000003814 drug Substances 0.000 title claims abstract description 28
- 238000010521 absorption reaction Methods 0.000 title claims abstract description 27
- 230000001737 promoting effect Effects 0.000 title claims abstract description 26
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 23
- 229960000074 biopharmaceutical Drugs 0.000 claims abstract description 22
- 150000001875 compounds Chemical class 0.000 claims abstract description 21
- 239000006041 probiotic Substances 0.000 claims abstract description 16
- 235000018291 probiotics Nutrition 0.000 claims abstract description 16
- 241000193830 Bacillus <bacterium> Species 0.000 claims abstract description 15
- 241000186840 Lactobacillus fermentum Species 0.000 claims abstract description 15
- 240000006024 Lactobacillus plantarum Species 0.000 claims abstract description 15
- 235000013965 Lactobacillus plantarum Nutrition 0.000 claims abstract description 15
- 241000186604 Lactobacillus reuteri Species 0.000 claims abstract description 15
- 229940012969 lactobacillus fermentum Drugs 0.000 claims abstract description 15
- 229940072205 lactobacillus plantarum Drugs 0.000 claims abstract description 15
- 229940001882 lactobacillus reuteri Drugs 0.000 claims abstract description 15
- 239000008213 purified water Substances 0.000 claims abstract description 13
- 241000901050 Bifidobacterium animalis subsp. lactis Species 0.000 claims abstract description 12
- 229940009289 bifidobacterium lactis Drugs 0.000 claims abstract description 12
- 241000235342 Saccharomycetes Species 0.000 claims abstract description 10
- 238000000855 fermentation Methods 0.000 claims description 50
- 230000004151 fermentation Effects 0.000 claims description 50
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 claims description 47
- 229920002558 Curdlan Polymers 0.000 claims description 39
- 239000001879 Curdlan Substances 0.000 claims description 39
- 229940078035 curdlan Drugs 0.000 claims description 39
- 235000019316 curdlan Nutrition 0.000 claims description 39
- 239000002131 composite material Substances 0.000 claims description 34
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 28
- 239000007788 liquid Substances 0.000 claims description 21
- 241000251468 Actinopterygii Species 0.000 claims description 20
- 239000000084 colloidal system Substances 0.000 claims description 20
- 235000013580 sausages Nutrition 0.000 claims description 16
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 15
- 229910052799 carbon Inorganic materials 0.000 claims description 15
- LUEWUZLMQUOBSB-FSKGGBMCSA-N (2s,3s,4s,5s,6r)-2-[(2r,3s,4r,5r,6s)-6-[(2r,3s,4r,5s,6s)-4,5-dihydroxy-2-(hydroxymethyl)-6-[(2r,4r,5s,6r)-4,5,6-trihydroxy-2-(hydroxymethyl)oxan-3-yl]oxyoxan-3-yl]oxy-4,5-dihydroxy-2-(hydroxymethyl)oxan-3-yl]oxy-6-(hydroxymethyl)oxane-3,4,5-triol Chemical compound O[C@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)O[C@@H](O[C@@H]2[C@H](O[C@@H](OC3[C@H](O[C@@H](O)[C@@H](O)[C@H]3O)CO)[C@@H](O)[C@H]2O)CO)[C@H](O)[C@H]1O LUEWUZLMQUOBSB-FSKGGBMCSA-N 0.000 claims description 14
- 229920002148 Gellan gum Polymers 0.000 claims description 14
- 229920002752 Konjac Polymers 0.000 claims description 14
- 235000010492 gellan gum Nutrition 0.000 claims description 14
- 239000000216 gellan gum Substances 0.000 claims description 14
- 235000010485 konjac Nutrition 0.000 claims description 14
- 229910052757 nitrogen Inorganic materials 0.000 claims description 14
- 239000002244 precipitate Substances 0.000 claims description 14
- VWDWKYIASSYTQR-UHFFFAOYSA-N sodium nitrate Chemical compound [Na+].[O-][N+]([O-])=O VWDWKYIASSYTQR-UHFFFAOYSA-N 0.000 claims description 14
- 239000002609 medium Substances 0.000 claims description 13
- 230000007935 neutral effect Effects 0.000 claims description 13
- 229920002581 Glucomannan Polymers 0.000 claims description 12
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 12
- 229940046240 glucomannan Drugs 0.000 claims description 12
- 239000006228 supernatant Substances 0.000 claims description 12
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 claims description 10
- 238000002360 preparation method Methods 0.000 claims description 10
- 239000013049 sediment Substances 0.000 claims description 10
- 238000003756 stirring Methods 0.000 claims description 10
- 229920000936 Agarose Polymers 0.000 claims description 9
- 239000003513 alkali Substances 0.000 claims description 9
- 235000013332 fish product Nutrition 0.000 claims description 9
- 230000000529 probiotic effect Effects 0.000 claims description 9
- 229920000858 Cyclodextrin Polymers 0.000 claims description 8
- 239000001116 FEMA 4028 Substances 0.000 claims description 8
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 8
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 8
- 229930006000 Sucrose Natural products 0.000 claims description 8
- WHGYBXFWUBPSRW-FOUAGVGXSA-N beta-cyclodextrin Chemical compound OC[C@H]([C@H]([C@@H]([C@H]1O)O)O[C@H]2O[C@@H]([C@@H](O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O3)[C@H](O)[C@H]2O)CO)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@@H]3O[C@@H]1CO WHGYBXFWUBPSRW-FOUAGVGXSA-N 0.000 claims description 8
- 235000011175 beta-cyclodextrine Nutrition 0.000 claims description 8
- 229960004853 betadex Drugs 0.000 claims description 8
- 239000008103 glucose Substances 0.000 claims description 8
- 239000005720 sucrose Substances 0.000 claims description 8
- MNNHAPBLZZVQHP-UHFFFAOYSA-N diammonium hydrogen phosphate Chemical compound [NH4+].[NH4+].OP([O-])([O-])=O MNNHAPBLZZVQHP-UHFFFAOYSA-N 0.000 claims description 7
- 229910000388 diammonium phosphate Inorganic materials 0.000 claims description 7
- 241000589158 Agrobacterium Species 0.000 claims description 6
- 229920002472 Starch Polymers 0.000 claims description 6
- 238000004108 freeze drying Methods 0.000 claims description 6
- 239000000314 lubricant Substances 0.000 claims description 6
- 238000002156 mixing Methods 0.000 claims description 6
- 239000008107 starch Substances 0.000 claims description 6
- 235000019698 starch Nutrition 0.000 claims description 6
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 5
- 240000008042 Zea mays Species 0.000 claims description 5
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 5
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 5
- 229910000019 calcium carbonate Inorganic materials 0.000 claims description 5
- 235000005822 corn Nutrition 0.000 claims description 5
- 239000002994 raw material Substances 0.000 claims description 5
- 230000001105 regulatory effect Effects 0.000 claims description 5
- 239000008367 deionised water Substances 0.000 claims description 4
- 229910021641 deionized water Inorganic materials 0.000 claims description 4
- 239000011780 sodium chloride Substances 0.000 claims description 4
- 238000005406 washing Methods 0.000 claims description 4
- 239000002671 adjuvant Substances 0.000 claims description 3
- 239000001963 growth medium Substances 0.000 claims description 3
- 229940068140 lactobacillus bifidus Drugs 0.000 claims description 3
- 239000012257 stirred material Substances 0.000 claims description 3
- 238000005303 weighing Methods 0.000 claims description 3
- 238000000605 extraction Methods 0.000 claims description 2
- 239000002054 inoculum Substances 0.000 claims description 2
- HQKMJHAJHXVSDF-UHFFFAOYSA-L magnesium stearate Chemical compound [Mg+2].CCCCCCCCCCCCCCCCCC([O-])=O.CCCCCCCCCCCCCCCCCC([O-])=O HQKMJHAJHXVSDF-UHFFFAOYSA-L 0.000 claims 2
- 108010011485 Aspartame Proteins 0.000 claims 1
- 229920000168 Microcrystalline cellulose Polymers 0.000 claims 1
- 241001052560 Thallis Species 0.000 claims 1
- IAOZJIPTCAWIRG-QWRGUYRKSA-N aspartame Chemical compound OC(=O)C[C@H](N)C(=O)N[C@H](C(=O)OC)CC1=CC=CC=C1 IAOZJIPTCAWIRG-QWRGUYRKSA-N 0.000 claims 1
- 235000010357 aspartame Nutrition 0.000 claims 1
- 239000000605 aspartame Substances 0.000 claims 1
- 229960003438 aspartame Drugs 0.000 claims 1
- 235000019359 magnesium stearate Nutrition 0.000 claims 1
- 235000019813 microcrystalline cellulose Nutrition 0.000 claims 1
- 239000008108 microcrystalline cellulose Substances 0.000 claims 1
- 229940016286 microcrystalline cellulose Drugs 0.000 claims 1
- 239000000546 pharmaceutical excipient Substances 0.000 claims 1
- 229940124531 pharmaceutical excipient Drugs 0.000 claims 1
- 239000000047 product Substances 0.000 claims 1
- 230000009286 beneficial effect Effects 0.000 abstract description 6
- 230000002708 enhancing effect Effects 0.000 abstract description 4
- 235000015816 nutrient absorption Nutrition 0.000 abstract description 4
- 230000007413 intestinal health Effects 0.000 abstract description 3
- 230000000052 comparative effect Effects 0.000 description 28
- 235000013372 meat Nutrition 0.000 description 18
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 12
- 210000000936 intestine Anatomy 0.000 description 10
- 238000000034 method Methods 0.000 description 8
- 201000006549 dyspepsia Diseases 0.000 description 7
- 230000000968 intestinal effect Effects 0.000 description 7
- 238000012360 testing method Methods 0.000 description 7
- 244000291564 Allium cepa Species 0.000 description 6
- 240000002234 Allium sativum Species 0.000 description 6
- 241000234314 Zingiber Species 0.000 description 6
- 235000006886 Zingiber officinale Nutrition 0.000 description 6
- 235000004611 garlic Nutrition 0.000 description 6
- 235000008397 ginger Nutrition 0.000 description 6
- 208000024891 symptom Diseases 0.000 description 6
- 235000010167 Allium cepa var aggregatum Nutrition 0.000 description 5
- 206010010774 Constipation Diseases 0.000 description 5
- 241000252233 Cyprinus carpio Species 0.000 description 5
- 238000010411 cooking Methods 0.000 description 5
- 239000000945 filler Substances 0.000 description 5
- 235000013305 food Nutrition 0.000 description 5
- 238000010438 heat treatment Methods 0.000 description 5
- 150000003839 salts Chemical class 0.000 description 5
- 230000000694 effects Effects 0.000 description 4
- 210000001035 gastrointestinal tract Anatomy 0.000 description 4
- 230000036039 immunity Effects 0.000 description 4
- 230000008855 peristalsis Effects 0.000 description 4
- 244000247812 Amorphophallus rivieri Species 0.000 description 3
- 235000001206 Amorphophallus rivieri Nutrition 0.000 description 3
- 206010012735 Diarrhoea Diseases 0.000 description 3
- 238000004458 analytical method Methods 0.000 description 3
- 238000011156 evaluation Methods 0.000 description 3
- 238000011081 inoculation Methods 0.000 description 3
- 239000000252 konjac Substances 0.000 description 3
- 230000000873 masking effect Effects 0.000 description 3
- 235000016709 nutrition Nutrition 0.000 description 3
- 210000002784 stomach Anatomy 0.000 description 3
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 2
- 241000282412 Homo Species 0.000 description 2
- 206010000059 abdominal discomfort Diseases 0.000 description 2
- 208000019790 abdominal distention Diseases 0.000 description 2
- 238000005119 centrifugation Methods 0.000 description 2
- 238000011049 filling Methods 0.000 description 2
- 230000035764 nutrition Effects 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 230000004044 response Effects 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- LDXJRKWFNNFDSA-UHFFFAOYSA-N 2-(2,4,6,7-tetrahydrotriazolo[4,5-c]pyridin-5-yl)-1-[4-[2-[[3-(trifluoromethoxy)phenyl]methylamino]pyrimidin-5-yl]piperazin-1-yl]ethanone Chemical compound C1CN(CC2=NNN=C21)CC(=O)N3CCN(CC3)C4=CN=C(N=C4)NCC5=CC(=CC=C5)OC(F)(F)F LDXJRKWFNNFDSA-UHFFFAOYSA-N 0.000 description 1
- 206010000060 Abdominal distension Diseases 0.000 description 1
- 235000002732 Allium cepa var. cepa Nutrition 0.000 description 1
- 208000019901 Anxiety disease Diseases 0.000 description 1
- 101100054292 Arabidopsis thaliana ABCG36 gene Proteins 0.000 description 1
- 101100351526 Arabidopsis thaliana PEN3 gene Proteins 0.000 description 1
- 208000035143 Bacterial infection Diseases 0.000 description 1
- 240000002791 Brassica napus Species 0.000 description 1
- 235000011293 Brassica napus Nutrition 0.000 description 1
- 235000000540 Brassica rapa subsp rapa Nutrition 0.000 description 1
- 208000024172 Cardiovascular disease Diseases 0.000 description 1
- 206010019233 Headaches Diseases 0.000 description 1
- 208000031361 Hiccup Diseases 0.000 description 1
- 206010020601 Hyperchlorhydria Diseases 0.000 description 1
- 206010029216 Nervousness Diseases 0.000 description 1
- 206010067171 Regurgitation Diseases 0.000 description 1
- 208000013738 Sleep Initiation and Maintenance disease Diseases 0.000 description 1
- 241000196252 Ulva Species 0.000 description 1
- 230000002159 abnormal effect Effects 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 201000009840 acute diarrhea Diseases 0.000 description 1
- 230000006978 adaptation Effects 0.000 description 1
- 230000036506 anxiety Effects 0.000 description 1
- 208000022362 bacterial infectious disease Diseases 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 239000001569 carbon dioxide Substances 0.000 description 1
- 229910002092 carbon dioxide Inorganic materials 0.000 description 1
- 208000026106 cerebrovascular disease Diseases 0.000 description 1
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 1
- 208000019902 chronic diarrheal disease Diseases 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 230000002526 effect on cardiovascular system Effects 0.000 description 1
- 230000029142 excretion Effects 0.000 description 1
- 235000013410 fast food Nutrition 0.000 description 1
- 239000013505 freshwater Substances 0.000 description 1
- 230000002496 gastric effect Effects 0.000 description 1
- 230000007160 gastrointestinal dysfunction Effects 0.000 description 1
- 239000003292 glue Substances 0.000 description 1
- 231100000869 headache Toxicity 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 206010022437 insomnia Diseases 0.000 description 1
- 230000001788 irregular Effects 0.000 description 1
- 235000012054 meals Nutrition 0.000 description 1
- 206010027175 memory impairment Diseases 0.000 description 1
- VUZPPFZMUPKLLV-UHFFFAOYSA-N methane;hydrate Chemical compound C.O VUZPPFZMUPKLLV-UHFFFAOYSA-N 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 210000004798 organs belonging to the digestive system Anatomy 0.000 description 1
- 238000003672 processing method Methods 0.000 description 1
- 230000002040 relaxant effect Effects 0.000 description 1
- 235000013599 spices Nutrition 0.000 description 1
- 230000009967 tasteless effect Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/66—Microorganisms or materials therefrom
- A61K35/74—Bacteria
- A61K35/741—Probiotics
- A61K35/744—Lactic acid bacteria, e.g. enterococci, pediococci, lactococci, streptococci or leuconostocs
- A61K35/745—Bifidobacteria
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L17/00—Food-from-the-sea products; Fish products; Fish meal; Fish-egg substitutes; Preparation or treatment thereof
- A23L17/70—Comminuted, e.g. emulsified, fish products; Processed products therefrom such as pastes, reformed or compressed products
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
- A23L29/20—Foods or foodstuffs containing additives; Preparation or treatment thereof containing gelling or thickening agents
- A23L29/206—Foods or foodstuffs containing additives; Preparation or treatment thereof containing gelling or thickening agents of vegetable origin
- A23L29/244—Foods or foodstuffs containing additives; Preparation or treatment thereof containing gelling or thickening agents of vegetable origin from corms, tubers or roots, e.g. glucomannan
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
- A23L29/20—Foods or foodstuffs containing additives; Preparation or treatment thereof containing gelling or thickening agents
- A23L29/269—Foods or foodstuffs containing additives; Preparation or treatment thereof containing gelling or thickening agents of microbial origin, e.g. xanthan or dextran
- A23L29/271—Curdlan; beta-1-3 glucan; Polysaccharides produced by agrobacterium or alcaligenes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
- A23L29/20—Foods or foodstuffs containing additives; Preparation or treatment thereof containing gelling or thickening agents
- A23L29/269—Foods or foodstuffs containing additives; Preparation or treatment thereof containing gelling or thickening agents of microbial origin, e.g. xanthan or dextran
- A23L29/272—Gellan
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/135—Bacteria or derivatives thereof, e.g. probiotics
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/14—Yeasts or derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/66—Microorganisms or materials therefrom
- A61K35/74—Bacteria
- A61K35/741—Probiotics
- A61K35/742—Spore-forming bacteria, e.g. Bacillus coagulans, Bacillus subtilis, clostridium or Lactobacillus sporogenes
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/66—Microorganisms or materials therefrom
- A61K35/74—Bacteria
- A61K35/741—Probiotics
- A61K35/744—Lactic acid bacteria, e.g. enterococci, pediococci, lactococci, streptococci or leuconostocs
- A61K35/747—Lactobacilli, e.g. L. acidophilus or L. brevis
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/06—Fungi, e.g. yeasts
- A61K36/062—Ascomycota
- A61K36/064—Saccharomycetales, e.g. baker's yeast
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/30—Macromolecular organic or inorganic compounds, e.g. inorganic polyphosphates
- A61K47/36—Polysaccharides; Derivatives thereof, e.g. gums, starch, alginate, dextrin, hyaluronic acid, chitosan, inulin, agar or pectin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/0012—Galenical forms characterised by the site of application
- A61K9/0053—Mouth and digestive tract, i.e. intraoral and peroral administration
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/06—Ointments; Bases therefor; Other semi-solid forms, e.g. creams, sticks, gels
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
- A61P1/14—Prodigestives, e.g. acids, enzymes, appetite stimulants, antidyspeptics, tonics, antiflatulents
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/07—Bacillus
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/225—Lactobacillus
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/225—Lactobacillus
- C12R2001/25—Lactobacillus plantarum
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/645—Fungi ; Processes using fungi
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Mycology (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Nutrition Science (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Pharmacology & Pharmacy (AREA)
- Animal Behavior & Ethology (AREA)
- Epidemiology (AREA)
- Zoology (AREA)
- Polymers & Plastics (AREA)
- Food Science & Technology (AREA)
- Biotechnology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Dispersion Chemistry (AREA)
- Molecular Biology (AREA)
- Virology (AREA)
- Tropical Medicine & Parasitology (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Botany (AREA)
- Biomedical Technology (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Marine Sciences & Fisheries (AREA)
- Inorganic Chemistry (AREA)
- Medical Informatics (AREA)
- Alternative & Traditional Medicine (AREA)
- Physiology (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
一种具有促进消化和吸收功能的生物药品，该生物药品包括以下组分：乳双歧杆菌、发酵乳杆菌、酵母菌、罗伊氏乳杆菌、植物乳杆菌、益生芽孢杆菌、复合食用胶和适量纯净水。本发明的生物药品实现了促进营养吸收保持肠道健康和提高人体消化能力的功能，有助于增强人体消化能力。
Description
技术领域
本发明涉及食品药品技术领域，尤其涉及一种具有促进消化和吸收功能的生物药品及其制备方法和应用。
背景技术
肠胃是人体的重要消化器官，是机体营养物质的消化吸收场所，也是对肠道有害物质进行免疫防护的重要部位。所有进入人体的食物都要经常肠胃吸收转化为营养传输给人体全身，肠胃蠕动慢，会造成对人体的吸收营养缓慢，发育不健康。现代人工作繁忙、进餐时间不定时、多吃快餐等问题都会引起胃肠道功能紊乱，临床表现主要在胃肠道涉及进食和排泄等方面的不正常，也常伴有失眠、焦虑、注意力涣散，健忘、神经过敏、头痛等其他功能性症状。
鱼类制品包括鱼丸、鱼糕、鱼肠等。制备鱼类制品往往以淡水鱼类为原料，而这种鱼肉往往会伴有“土腥味”。目前去除鱼肉腥味的加工方法主要是加入香料进行掩盖，例如葱姜蒜辣，但这种方法也容易掩盖掉鱼肉本身的鲜美味道。为了达到鱼类制品的软弹口感，往往会加入食用胶，要求既能增强鱼类制品的口感，又要能保留鱼肉的自身鲜美口味和营养成分。除此之外，因为鱼类制品的口感软弹，近些年来喜欢吃鱼类制品的儿童数量逐年增多。但是，儿童经常或过量的食用鱼类制品，容易出现腹胀、胃部不适等消化不良的症状。
本发明提供了一种生物药品，该生物药品具有促进消化和吸收的功能，且更吸引儿童食用。
发明内容
本发明提供了一种具有促进消化和吸收功能的生物药品。
一种具有促进消化和吸收功能的生物药品，该生物药品包括以下组分：乳双歧杆菌、发酵乳杆菌、酵母菌、罗伊氏乳杆菌、植物乳杆菌、益生芽孢杆菌、复合食用胶和适量纯净水；
以上组分的份数比例为：乳双歧杆菌：5-10份；发酵乳杆菌：20-30份；酵母菌：10-20份；罗伊氏乳杆菌：20-30份；植物乳杆菌：5-10份；益生芽孢杆菌：5-10份；粉末状复合食用胶：40-80份。
一种具有促进消化和吸收功能的生物药品的制备方法，包括有以下步骤：
步骤（a）：称取要求比例份数的乳双歧杆菌、发酵乳杆菌、酵母菌、罗伊氏乳杆菌、植物乳杆菌、益生芽孢杆菌和粉末状复合食用胶、纯净水，备用；
步骤（b）：将乳双歧杆菌、发酵乳杆菌、酵母菌、罗伊氏乳杆菌、植物乳杆菌、益生芽孢杆菌和粉末状复合食用胶放入搅拌机中在15-35℃下搅拌均匀，得到匀拌原料，备用；
步骤（c）：将匀拌原料中加入纯净水和药用辅料，放入搅拌机中在15-35℃下搅拌均匀，得到凝胶状生物药品，备用；
步骤（d）：将凝胶状生物药品按剂量单独进行封装。
本发明具有以下有益效果：
（1）本发明的生物药品实现了促进营养吸收保持肠道健康和提高人体消化能力的功能，有助于增强人体消化能力。
（2）本发明的复合食用胶的发酵培养基液中，以葡萄糖、蔗糖、琼脂糖为复合碳源，以NH4Cl、(NH4)2HPO4、NaNO3为复合氮源，且加入β-环糊精和酒糟，可明显提升可得然胶的产量。复合食用胶在碱液提取过程中，加入结冷胶，一次离心后，再加入魔芋葡甘露聚糖，有助于使可得然胶、结冷胶和魔芋葡甘露聚糖形成更加稳定的结构，使后续冻干得到的复合食用胶性能稳定，生物药品口感更加的软弹，且更吸引儿童食用。
具体实施方式
下面将结合本发明的实施例，对本发明实施例中的技术方案进行清楚、完整地描述，显然，所描述的实施例仅仅是本发明一部分实施例，而不是全部的实施例。基于本发明中的实施例，本领域普通技术人员在没有做出创造性劳动前提下所获得的所有其他实施例，都属于本发明保护的范围。
实施例1
具有促进消化和吸收功能的生物药品，该生物药品包括以下组分：乳双歧杆菌、发酵乳杆菌、酵母菌、罗伊氏乳杆菌、植物乳杆菌、益生芽孢杆菌、复合食用胶和适量纯净水。
实施例2
具有促进消化和吸收功能的生物药品的制备方法，包括有以下步骤：
步骤（a）：称取要求比例份数的乳双歧杆菌、发酵乳杆菌、酵母菌、罗伊氏乳杆菌、植物乳杆菌、益生芽孢杆菌和粉末状复合食用胶、纯净水，备用；
步骤（b）：将乳双歧杆菌、发酵乳杆菌、酵母菌、罗伊氏乳杆菌、植物乳杆菌、益生芽孢杆菌和粉末状复合食用胶放入搅拌机中在15-35℃下搅拌均匀，得到匀拌原料，备用；
步骤（c）：将匀拌原料中加入纯净水和药用辅料，放入搅拌机中在15-35℃下搅拌均匀，得到凝胶状生物药品，备用；
步骤（d）：将凝胶状生物药品按剂量单独进行封装。
实施例3
生物药品，组分的份数比例为：乳双歧杆菌：5份；发酵乳杆菌：20份；酵母菌：10份；罗伊氏乳杆菌：20份；植物乳杆菌：5份；益生芽孢杆菌：5份；粉末状复合食用胶：40份，纯净水适量。利用实施例2的方法制备成具有促进消化和吸收功能的生物药品。
实施例3中复合食用胶的制备方法，包括以下步骤：
步骤（1）制备发酵培养基液，所述发酵培养基液包括复合碳源10%、复合氮源0.5%、MgS04·7H2O0.2%、CaCO30.2%、β-环糊精3%、酒糟2%、玉米浆0.5%，其他为水，调pH为中性。在5L的发酵罐中加入3L的发酵培养基液，加入土壤杆菌菌株，菌株接种量为5%，发酵培养温度为25℃，转速为150r/min，培养周期为72h，得到可得然胶发酵液。其中：复合碳源中，葡萄糖、蔗糖、琼脂糖的质量比为1：1：0.2；复合氮源中，NH4Cl、(NH4)2HPO4、NaNO3的质量比为1：2：1。
步骤（2）将可得然胶发酵液离心后，离心转速5000r/min，收集沉淀，得到可得然胶发酵液沉淀。
步骤（3）将可得然胶发酵液沉淀加0.2M的NaOH碱液提取后，测得每克可得然胶发酵液可提取的可得然胶的预计质量为可得然胶的质量。本实施例1中可得然胶的质量为0.76g。以可得然胶、结冷胶、魔芋葡甘露聚糖的质量比为1：1：1计算。在可得然胶发酵液沉淀中加入结冷胶混合均匀后，在其中加入0.2M的NaOH碱液提取搅拌均匀后，离心除去不溶性菌体，取上清液加入CaCl2、NaCl和魔芋葡甘露聚糖后，再次离心，得到胶体沉淀物，两次离心转速均为5000r/min。CaCl2加入量分别为上清液质量的0.2%、NaCl加入量分别为上清液质量的0.6%。
步骤（4）将胶体沉淀物用去离子水洗涤后，离心脱水，离心转速5000r/min，得到中性胶体。
步骤（5）将中性胶体冷冻干燥粉碎，得到粉末状复合食用胶。
实施例4
生物药品，组分的份数比例为：乳双歧杆菌：10份；发酵乳杆菌：30份；酵母菌：20份；罗伊氏乳杆菌：30份；植物乳杆菌：10份；益生芽孢杆菌：10份；粉末状复合食用胶：80份，纯净水适量。利用实施例2的方法制备成具有促进消化和吸收功能的生物药品。
实施例4中复合食用胶的制备方法，包括以下步骤：
步骤（1）制备发酵培养基液，所述发酵培养基液包括复合碳源10%、复合氮源0.5%、MgS04·7H2O0.2%、CaCO30.2%、β-环糊精3%、酒糟2%、玉米浆0.5%，其他为水，调pH为中性。在5L的发酵罐中加入3L的发酵培养基液，加入土壤杆菌菌株，菌株接种量为5%，发酵培养温度为25℃，转速为150r/min，培养周期为72h，得到可得然胶发酵液。其中：复合碳源中，葡萄糖、蔗糖、琼脂糖的质量比为1：0.3：0.1；复合氮源中，NH4Cl、(NH4)2HPO4、NaNO3的质量比为1：1：0.5。
步骤（2）将可得然胶发酵液离心后，离心转速5000r/min，收集沉淀，得到可得然胶发酵液沉淀。
步骤（3）以可得然胶、结冷胶、魔芋葡甘露聚糖的质量比为1：1：1，计算结冷胶和魔芋葡甘露聚糖的质量。在可得然胶发酵液沉淀中加入结冷胶混合均匀后，在其中加入0.2M的NaOH碱液提取搅拌均匀后，离心除去不溶性菌体，取上清液加入CaCl2、NaCl和魔芋葡甘露聚糖后，再次离心，得到胶体沉淀物，两次离心转速均为5000r/min。CaCl2加入量分别为上清液质量的0.1%、NaCl加入量分别为上清液质量的0.5%。
步骤（4）将胶体沉淀物用去离子水洗涤后，离心脱水，离心转速5000r/min，得到中性胶体。
步骤（5）将中性胶体冷冻干燥粉碎，得到粉末状复合食用胶。
实施例5
生物药品，组分的份数比例为：乳双歧杆菌：8份；发酵乳杆菌：25份；酵母菌：15份；罗伊氏乳杆菌：25份；植物乳杆菌：8份；益生芽孢杆菌：8份；粉末状复合食用胶：60份，纯净水适量。利用实施例2的方法制备成具有促进消化和吸收功能的生物药品。
实施例5中复合食用胶的制备方法，包括以下步骤：
步骤（1）制备发酵培养基液，所述发酵培养基液包括复合碳源10%、复合氮源0.5%、MgS04·7H2O0.2%、CaCO30.2%、β-环糊精3%、酒糟2%、玉米浆0.5%，其他为水，调pH为中性。在5L的发酵罐中加入3L的发酵培养基液，加入土壤杆菌菌株，菌株接种量为5%，发酵培养温度为25℃，转速为150r/min，培养周期为72h，得到可得然胶发酵液。其中：复合碳源中，葡萄糖、蔗糖、琼脂糖的质量比为1：1：0.5；复合氮源中，NH4Cl、(NH4)2HPO4、NaNO3的质量比为1：3：1。
步骤（2）将可得然胶发酵液离心后，离心转速5000r/min，收集沉淀，得到可得然胶发酵液沉淀。
步骤（3）以可得然胶、结冷胶、魔芋葡甘露聚糖的质量比为1：2：2，计算结冷胶和魔芋葡甘露聚糖的质量。在可得然胶发酵液沉淀中加入结冷胶混合均匀后，在其中加入0.2M的NaOH碱液提取搅拌均匀后，离心除去不溶性菌体，取上清液加入CaCl2、NaCl和魔芋葡甘露聚糖后，再次离心，得到胶体沉淀物，两次离心转速均为5000r/min。CaCl2加入量分别为上清液质量的0.3%、NaCl加入量分别为上清液质量的0.8%。
步骤（4）将胶体沉淀物用去离子水洗涤后，离心脱水，离心转速5000r/min，得到中性胶体。
步骤（5）将中性胶体冷冻干燥粉碎，得到粉末状复合食用胶。
实施例3-中实施例5中发酵液的组分不仅仅限定为上述提供的范围，还可以是复合碳源8-12%、复合氮源0.5-1%、MgS04·7H2O0.1-0.3%、CaCO30.1-0.3%、β-环糊精1-3%、酒糟1-3%、玉米浆0.5-0.8%，其他为水，调pH为中性。在发酵罐中加入发酵培养基液，加入土壤杆菌菌株，菌株接种量为5-8%，发酵培养温度为25-35℃，转速为120-150r/min，培养周期为72-96h。通过对发酵液各组分的用量调整发现，复合碳源和复合氮源下，可得然胶的产胶量远远高于常规的单一碳源、单一氮源。尤其是复合碳源中琼脂糖的加入，比不加入琼脂糖的同比例复合碳源，可得然胶的产量高了5-10%。
实施例3-实施例5中：
乳双歧杆菌的作用通常有维持肠道菌群平衡、辅助改善消化不良、辅助改善腹泻、辅助改善便秘、辅助提高免疫力等。
发酵乳杆菌具有一定的调节肠道菌群、增强免疫力和促进消化吸收等作用与功效。
酵母菌可以帮助机体分解二氧化碳和水，可以促进肠道蠕动，有利于食物发酵，有润肠通便的作用，从而缓解消化不良、便秘等不适症状。
罗伊氏乳杆菌是肠道益生菌，罗伊氏乳杆菌可以调节肠道菌群、减少细菌感染，还可以缓解便秘以及促进消化、吸收等。
植物乳酸菌可以调节免疫，抑制病原菌，降低血清胆固醇，预防心脑血管疾病。维持肠道菌群平衡，促进营养吸收，用于因肠道菌群失调引起的急慢性腹泻、便秘、消化不良、腹胀。
益生芽孢杆菌可以促进胃肠道的蠕动，有助于食物的消化与吸收，具有辅助改善消化不良的作用，可以提高身体的免疫力，能够促进胃肠道的蠕动，有利于食物的消化与吸收，具有辅助改善便秘的作用。
复合食用胶的发酵培养基液中，以葡萄糖、蔗糖、琼脂糖为复合碳源，以NH4Cl、(NH4)2HPO4、NaNO3为复合氮源，且加入β-环糊精和酒糟，可明显提升可得然胶的产量。复合食用胶在碱液提取过程中，加入结冷胶，一次离心后，再加入魔芋葡甘露聚糖，有助于使可得然胶、结冷胶和魔芋葡甘露聚糖形成更加稳定的结构，使后续冻干得到的复合食用胶性能稳定，生物药品口感更加的软弹，且更吸引儿童食用。
因此，本发明的实施例1-实施例5的生物药品实现了促进营养吸收保持肠道健康和提高人体消化能力的功能，有助于增强人体消化能力。
对比例1
将实施例3中的复合食用胶替换为芜湖金丰禾生物科技有限公司品牌的食用胶，利用实施例2的方法制备成生物药品，得到对比例1生物药品。
对比例2
将实施例5中的复合食用胶替换为陕西云升生物科技有限公司品牌的可得然胶、利用实施例2的方法制备成生物药品，得到对比例2生物药品。
实施例3、实施例5、对比例1和对比例2的评价试验分析：
通过20个试吃志愿者蒙眼闻气味发现：所有志愿者都表示实施例3、实施例5、对比例1和对比例2制备的生物药品口感差距明显，一致认为实施例3和实施例5的生物药品口感更加的软弹，且更吸引儿童食用。
应用例1
具有促进消化和吸收功能的生物药品的应用，将实施例3的生物药品、淀粉、鲤鱼肉、葱姜蒜盐混合打成肉泥，使用灌肠机将肉泥灌入肠衣，加热煮熟，制备成鱼肠。
应用例2
具有促进消化和吸收功能的生物药品的应用，将实施例4的生物药品、淀粉、鲤鱼肉、葱姜蒜盐混合打成肉泥，使用灌肠机将肉泥灌入肠衣，加热煮熟，制备成鱼肠。
应用例3
具有促进消化和吸收功能的生物药品的应用，将实施例5的生物药品、淀粉、鲤鱼肉、葱姜蒜盐混合打成肉泥，使用灌肠机将肉泥灌入肠衣，加热煮熟，制备成鱼肠。
将应用例1、应用例2和应用例3制备的鱼肠，分别随机抽取1/3，组成应用例鱼肠。
对比例3：将市购芜湖金丰禾生物科技有限公司品牌的食用胶、淀粉、鲤鱼肉、葱姜蒜盐混合打成肉泥，使用灌肠机将肉泥灌入肠衣，加热煮熟，制备成对比例3鱼肠。
对比例4：将市购陕西云升生物科技有限公司品牌的可得然胶、淀粉、鲤鱼肉、葱姜蒜盐混合打成肉泥，使用灌肠机将肉泥灌入肠衣，加热煮熟，制备成对比例4鱼肠。
应用例、对比例3和对比例4的评价试验分析：
通过20个试吃志愿者蒙眼闻气味发现：所有志愿者都表示应用例、对比例3和对比例4制备的鱼肠闻起来腥味差距明显，一致认为应用例在制备的鱼肠腥味明显小。将应用例和对比例3-2制备的鱼肠使用具有PEN3便携电子鼻进行检测，发现应用例制备的鱼肠对5个气味传感器的响应值都比对比例3低，对10个气味传感器的响应值都比对比例4低。
通过20个试吃志愿者随机品尝发现：4个志愿者都表示应用例、对比例3和对比例4制备的鱼肠品尝起来口感差距不大，16个志愿者认为应用例在制备的鱼肠口感更加的软弹。
通过20个试吃志愿者随机品尝发现：2个志愿者都表示应用例、对比例3和对比例4制备的鱼肠同等用量后症状差距不大。18个志愿者都表示对比例3和对比例4制备的鱼肠同等用量后，出现腹胀、胃部不适、打嗝、嗳气、反酸、胃酸过多等消化不良的症状，而应用例制备的鱼肠同等用量后，没有出现消化不良的症状。
综上应用例、对比例3和对比例4的评价试验分析，利用本发明生物药品制备的鱼类制品，能够去除土腥味，保留鱼肉鲜美口味，同时口感软弹，具有促进消化和吸收功能。
本领域技术人员在考虑说明书及实践这里发明的公开后，将容易想到本发明的其它实施方案。本发明旨在涵盖本发明的任何变型、用途或者适应性变化，这些变型、用途或者适应性变化遵循本发明的一般性原理并包括本发明未公开的本技术领域中的公知常识或惯用技术手段。说明书和实施例仅被视为示例性的，本发明的真正范围和精神由下面的权利要求指出。
以上所述的本发明实施方式并不构成对本发明保护范围的限定。
Claims (3)
1.一种具有促进消化和吸收功能的生物药品，其特征在于，该生物药品原料为：适量纯净水；乳双歧杆菌：5-10份；发酵乳杆菌：20-30份；酵母菌：10-20份；罗伊氏乳杆菌：20-30份；植物乳杆菌：5-10份；益生芽孢杆菌：5-10份；粉末状复合食用胶：40-80份；
该生物药品的制备方法，包括有以下步骤：
步骤（a）：称取要求比例份数的乳双歧杆菌、发酵乳杆菌、酵母菌、罗伊氏乳杆菌、植物乳杆菌、益生芽孢杆菌和粉末状复合食用胶、纯净水，备用；
复合食用胶的制备方法，包括以下步骤：
步骤（1），以土壤杆菌为菌株，以葡萄糖、蔗糖、琼脂糖为复合碳源，以NH4Cl、(NH4)2HPO4、NaNO3为复合氮源，并在发酵培养基液中加入β-环糊精和酒糟，发酵得到可得然胶发酵液；
步骤（1）包括：制备发酵培养基液，所述发酵培养基液包括复合碳源8-12%、复合氮源0.5-1%、MgS04·7H2O0.1-0.3%、CaCO30.1-0.3%、β-环糊精1-3%、酒糟1-3%、玉米浆0.5-0.8%，其他为水，调pH为中性；在发酵罐中加入发酵培养基液，加入土壤杆菌菌株，菌株接种量为5-8%，发酵培养温度为25-35℃，转速为120-150r/min，培养周期为72-96h；
步骤（1）的复合碳源中，所述葡萄糖、蔗糖、琼脂糖的质量比为1：（0.3-1）：（0.1-0.5）；所述复合氮源中，NH4Cl、(NH4)2HPO4、NaNO3的质量比为1：（1-3）：（0.5-1）；
步骤（2），将可得然胶发酵液离心后，收集沉淀，得到可得然胶发酵液沉淀；
步骤（3），在可得然胶发酵液沉淀中加入结冷胶混合均匀后，在其中加入碱液，搅拌均匀后，离心除去不溶性菌体，取上清液加入CaCl2、NaCl和魔芋葡甘露聚糖后，再次离心，得到胶体沉淀物；
步骤（3）包括：将可得然胶发酵液沉淀加碱液提取后，测得每克可得然胶发酵液沉淀可提取的可得然胶的预计质量为可得然胶的质量，所述可得然胶、结冷胶、魔芋葡甘露聚糖的质量比为1：（1-2）：（1-2）；碱液浓度为0.2-0.4mol/L；CaCl2加入量分别为上清液质量的0.1-0.3%、NaCl加入量分别为上清液质量的0.5-0.8%；
步骤（4）将胶体沉淀物用去离子水洗涤后，离心脱水，得到中性胶体；
步骤（5）将中性胶体冷冻干燥粉碎，得到粉末状复合食用胶；
步骤（b）：将乳双歧杆菌、发酵乳杆菌、酵母菌、罗伊氏乳杆菌、植物乳杆菌、益生芽孢杆菌和粉末状复合食用胶放入搅拌机中在15-35℃下搅拌均匀，得到匀拌原料，备用；
步骤（c）：将匀拌原料中加入纯净水和药用辅料，放入搅拌机中在15-35℃下搅拌均匀，得到凝胶状生物药品，备用；
步骤（d）：将凝胶状生物药品按剂量单独进行封装。
2.根据权利要求1所述的具有促进消化和吸收功能的生物药品，其特征在于，步骤（c）中的药用辅料选自淀粉、葡萄糖、蔗糖、阿司帕坦、微晶纤维素、硬脂酸镁的一种或多种。
3.一种基于权利要求1或2所述的具有促进消化和吸收功能的生物药品的应用，其特征在于，将该生物药品加入鱼类制品中，制备成具有促进消化和吸收功能的鱼丸、鱼糕或鱼肠。
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202311509496.0A CN117547561B (zh) | 2023-11-14 | 2023-11-14 | 一种具有促进消化和吸收功能的生物药品 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202311509496.0A CN117547561B (zh) | 2023-11-14 | 2023-11-14 | 一种具有促进消化和吸收功能的生物药品 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN117547561A CN117547561A (zh) | 2024-02-13 |
| CN117547561B true CN117547561B (zh) | 2024-04-30 |
Family
ID=89815975
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202311509496.0A Active CN117547561B (zh) | 2023-11-14 | 2023-11-14 | 一种具有促进消化和吸收功能的生物药品 |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN117547561B (zh) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101797050A (zh) * | 2010-02-26 | 2010-08-11 | 丹尼斯克（中国）有限公司 | 一种含有微生物的胶冻微粒及其制备方法和用途 |
| CN112438402A (zh) * | 2020-12-16 | 2021-03-05 | 拉格比(广东)健康科技有限公司 | 一种复合益生菌及其制备方法 |
| CN115868609A (zh) * | 2022-09-09 | 2023-03-31 | 福建富邦食品有限公司 | 富含益生菌丸子及其制备方法 |
| CN116918891A (zh) * | 2023-07-24 | 2023-10-24 | 晋江市益利发食品有限公司 | 一种含益生菌的无糖软糖及其制备方法 |
-
2023
- 2023-11-14 CN CN202311509496.0A patent/CN117547561B/zh active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101797050A (zh) * | 2010-02-26 | 2010-08-11 | 丹尼斯克（中国）有限公司 | 一种含有微生物的胶冻微粒及其制备方法和用途 |
| CN112438402A (zh) * | 2020-12-16 | 2021-03-05 | 拉格比(广东)健康科技有限公司 | 一种复合益生菌及其制备方法 |
| CN115868609A (zh) * | 2022-09-09 | 2023-03-31 | 福建富邦食品有限公司 | 富含益生菌丸子及其制备方法 |
| CN116918891A (zh) * | 2023-07-24 | 2023-10-24 | 晋江市益利发食品有限公司 | 一种含益生菌的无糖软糖及其制备方法 |
Non-Patent Citations (1)
| Title |
|---|
| 益生菌的益生作用分析;冯震等;《中外企业家》;20151025(第30期);第251页 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN117547561A (zh) | 2024-02-13 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102090560B (zh) | Gos和多聚果糖的协同作用 | |
| CN104642870B (zh) | 一种益生元组合物 | |
| CN103445048B (zh) | 含有益生菌的大豆寡糖产品及其制备方法 | |
| Tian et al. | Digestive characteristics of Hericium erinaceus polysaccharides and their positive effects on fecal microbiota of male and female volunteers during in vitro fermentation | |
| CN101185511A (zh) | 具有肠胃微生态调节作用的保健方便面食品的制作方法 | |
| CN106690331A (zh) | 一种海带膳食纤维颗粒及其制备方法 | |
| CN105558737A (zh) | 一种无糖型食用菌固体饮料及其制备方法 | |
| CN112877172A (zh) | 营养健康黑醋汁制作方法 | |
| CN105661515B (zh) | 一种防治三高症的酵素含片及其制备方法 | |
| CN109315769A (zh) | 一种用于改善人体肠内环境的组合物及其制备方法 | |
| CN114521590A (zh) | 阻断糖、油脂吸收的白芸豆益生菌减脂片及其制备方法 | |
| CN117547561B (zh) | 一种具有促进消化和吸收功能的生物药品 | |
| CN112931883A (zh) | 一种益生元组合物及其制备方法和应用 | |
| CN101496568A (zh) | 一种绿豆或豌豆膳食纤维及其生产方法 | |
| KR102296397B1 (ko) | 꽃송이버섯 발효 젤리의 제조방법 및 이로부터 제조되는 꽃송이버섯 발효 젤리 | |
| CN107361355A (zh) | 一种红枣酵素粉的制备方法 | |
| CN114344341B (zh) | 具有调节肠道作用的复合益生菌组合物及制备方法和应用 | |
| CN113749256B (zh) | 大蒜多糖在调节肠道菌群功能中的应用 | |
| CN108991527A (zh) | 一种增强免疫力破壁灵芝孢子粉胶囊 | |
| JP2010124720A (ja) | 植物性乳酸菌増殖促進用組成物及び経口摂取用組成物 | |
| KR102296402B1 (ko) | 꽃송이버섯 발효 비드를 포함하는 젤리의 제조방법 및 이로부터 제조되는 꽃송이버섯 발효 비드를 포함하는 젤리 | |
| CN107467654A (zh) | 一种海带海参复合提取物及其制备方法和应用 | |
| CN115777948B (zh) | 一种适合ibs患者的膳食纤维补充剂 | |
| KR101229092B1 (ko) | 적석지를 이용한 미생물 유래 셀룰로스의 제조방법 및 이를 이용한 다이어트용 건강보조식품 조성물 | |
| KR20140039525A (ko) | 장 기능 및 변비 개선 효과를 나타내는 유산균을 이용한 치커리 화이바 발효물의 제조방법 |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |