CN117413768A - Method for breeding blue wheat variety by using blue dwarf male-sterile main and auxiliary double-round group selection - Google Patents

Abstract

The invention relates to the field of wheat breeding, and discloses a method for breeding blue wheat varieties by using blue dwarf male-sterile main and auxiliary double-round group selection, which comprises the following steps: through the design of the auxiliary round selection group of the blue dwarf-male-sterile blue wheat, a technical system for breeding blue wheat varieties by utilizing the correlated blue dwarf-male-sterile round selection double-group five-garden areas is formed; the blue grains separated by the single blue dwarf male sterile line of the main group and the secondary group are used for each round of blue dwarf male sterile line of the main group and the secondary group, and the two-group character level is synchronously improved for each round of blue dwarf male sterile line; by establishing and running the blue dwarf-male-sterile main-auxiliary double-round selection group, the design of the blue dwarf-male-sterile auxiliary-round selection group is free from the limitation of low linkage probability and few types of blue wheat varieties selected from non-dwarf-male-sterile plants which are only selected from the blue dwarf-male-sterile groups to break the linkage, and by establishing the blue dwarf-male-sterile auxiliary-round selection group, all genetic types of the main group are reserved in the blue wheat variety types, so that the blue wheat variety types are enriched, the opportunity of selecting breakthrough blue wheat varieties is increased, and the blue wheat variety is the most main means for creating blue wheat germplasm innovation.

Description

Method for breeding blue wheat variety by using blue dwarf male-sterile main and auxiliary double-round group selection

Technical Field

The invention relates to the technical field of wheat breeding, in particular to a method for breeding blue wheat varieties by utilizing blue dwarf male-female major-minor double-round group selection.

Background

Referring to the data, no public report and patent application for the related research content of blue wheat variety breeding by utilizing blue dwarf-male-sterile main and auxiliary double-round group selection are found up to date.

The important germplasm 'blue dwarf-male-sterile' used by the invention is an abnormal translocation additional system monomer with three dominant characters of blue grain (4D/4E), dwarf (Rht 10) and anther abortion (Ms 2) linked on a 4D chromosome, and is an ideal tool carrier for wheat rotation selection. The application of blue dwarf-male-sterile is mainly used for hybrid seed production by utilizing wheat heterosis besides being used as a hybridization tool, and has the function of a wheat germplasm innovation platform. The method uses a common white grain variety as a male parent for pollination, blue grains (accounting for about 1/4) and white grains (accounting for about 3/4) are planted on the same wheat ears, the blue grains are sorted to be planted as dwarf and sterile, and the pollination performance of the white grain variety is still the same as that of the previous generation, and the blue grains and the white grains are planted on the same wheat ears; the plant height of the white grains after planting and sorting is normal, anther selfing and setting are carried out, all the set seeds are white grains which are not different from common varieties, and the next generation of the white grains is still carried out after the white grains are continuously planted. However, when the blue wheat is used as a male parent and pollinated by 'blue dwarf male', all the seeds are blue grains, and almost all the offspring are fertile (the principle is yet to be researched), which is the realistic basis for breeding blue wheat varieties by establishing blue wheat auxiliary round selection groups.

Recurrent selection is an important approach for population improvement and germplasm innovation, and is difficult to realize due to small amphiprotic homoflowers of wheat flowers. Since Liu in the national academy of sciences of China developed "dwarf-male-sterile", a new way for establishing a wheat recurrent selection group and carrying out germplasm innovation and wheat variety breeding through wheat recurrent selection was opened up. However, the alternate population established by using dwarf-male-sterile is required to identify non-dwarf fertile plants in the extracted population after the extraction, the fertility of the dwarf-male-sterile cannot be distinguished before the seed is placed in the ground, the sterile line and the male parent cannot be respectively sowed and selected, and the sterile line and the male parent are separately planted and respectively selected by virtue of the granular mark before the seed is placed in the ground. The wheat alternate selection group is more convenient and efficient to construct by utilizing the blue dwarf male-sterile, the operability is stronger, and the selection efficiency is higher. Geng Aimin and its team have reported that the blue grain near isogenic line of any wheat variety is bred by blue dwarf-male-sterile selection (reporting 12 months 2015, obtaining patent authorization in 6 months of 2018, patent number CN 105340730B), and that the blue dwarf-male-sterile selection group is utilized to breed patents on the aspect of the method of breeding different wheat germplasm such as blue dwarf wheat (reporting 8 months 2015, reporting 1 month of 20183B; reporting 10 months 2020, and reporting 8 months 2023, patent number CN 111903504B), but those patents are all realized by searching various plants which break gene linkage segregation in the selection group. But the blue wheat variety is selected by searching the blue wheat strain which breaks linkage separation in the round selection group, the probability of breaking linkage is low, the types are few, the abundance of genetic basis is lacking, the narrow-width quantity is small, and the breeding requirements of quick breeding type-rich, innovative and breakthrough blue wheat variety cannot be met. The invention designs the blue dwarf-male-sterile main-auxiliary double-round selection group which is synchronously lifted by mutual association, in particular to the design of the blue dwarf-male-sterile blue wheat auxiliary-round selection group, enriches the germplasm types and genetic basic breadth of blue wheat, ensures that the germplasm of blue wheat is synchronously improved with the germplasm of common wheat, lays a genetic foundation for the breeding of breakthrough blue wheat varieties, and provides a method guarantee.

Disclosure of Invention

Recurrent selection is an important means for population improvement and germplasm innovation, and is difficult to realize due to small amphiprotic homoflowers of wheat flower devices. The invention aims to enrich the germplasm types of blue wheat by means of a method of recurrent selection of blue dwarf male-sterile, and increase the chances of breeding breakthrough blue wheat varieties.

In view of the above, the invention provides a method for breeding blue wheat varieties by using blue dwarf male-female major-minor double-round group selection, which comprises the following steps:

through the design of the auxiliary round selection group of the blue dwarf-male-sterile blue wheat, a technical system for breeding blue wheat varieties by utilizing the five nursery areas of the mutually related blue dwarf-male-sterile double groups is formed;

the blue dwarf-male-sterile double-group five-nursery region comprises a main group A region, a main group B region, a double-group C region, a secondary group D region and a secondary group E region;

blue grains separated by the blue dwarf male sterile line of the main group and the secondary group are used for each round of selection of the blue dwarf male sterile line of the main group, and the character level of the two groups is synchronously improved for each round of selection.

Further, the main group A region comprises a blue dwarf male-sterile round-selection group main group planting seed selection garden; the main group B region comprises a main wheel group wheat variety selection nursery; the double-group C region comprises seed selection gardens from the main group A region, the double-group C region, the auxiliary group D region, the auxiliary group E region and 4 blue wheat regions; wherein the secondary group D region is a male parent; the secondary group D region comprises a blue dwarf-male-sterile blue wheat secondary group selection planting garden; the secondary group E region comprises a secondary group seed selfing and expanding group nursery.

Further, the main group A region, the main group B region, the double group C region, the auxiliary group D region and the auxiliary group E region respectively comprise respective isolation regions.

Further, the main group B region needs to be isolated from any blue wheat region;

the double-group C region and the auxiliary group E region are blue wheat planting regions which can be adjacently planted in the same isolation region, and can share one isolation region with the auxiliary group D region of the blue wheat alternate auxiliary group.

Further, the main group A region takes blue dwarf male parent and common white grain wheat variety as male parent to form a blue dwarf male parent group, the blue dwarf male parent is selected, and the blue dwarf male parent is provided for the main and the auxiliary alternative male parent in turn;

the main group B region is based on white grains of main group blue dwarf-male-sterile pollination, seed selection is carried out for the main group and male parents are provided for the main group in turn, and the major line material is selected and bred into a new variety of common white grain wheat;

the double-group C region carries out planting selection on blue wheat basic materials from all groups except the main group B region, breeds blue wheat varieties, and provides male parent for blue dwarf-male-sterile blue wheat auxiliary groups in turn;

the secondary group D area updates the blue dwarf male parent and the blue dwarf female parent in turn to generate blue wheat seed selection germplasm resources with wide genetic basis and rich types;

and sowing the seed cells provided by the auxiliary group E area, carrying out continuous two-generation selfing group expansion, selecting full blue single ears, and sending the planting ear rows of the double group C area to carry out subsequent breeding to obtain blue wheat varieties.

Further, the whole blue selection in the secondary group E region and the whole blue selection from the male parent in the secondary group D region are fed into the double group C region, because the blue plant from the main group A region and the whole blue selection from the secondary group E region and the secondary group D region are planted in the double group C region

Further, the operation method of the main group A area is as follows: according to the recurrent selection method, selecting the blue dwarf single plants by each recurrent generation of the recurrent group, sowing male parents on two sides of a blue dwarf sterile line, forming two kinds of grains with different colors of blue grains and white grains on the same wheat ear after blue dwarf pollination, carrying out grain color sorting on the blue dwarf single plants, and sorting the blue grains as the blue dwarf of the next recurrent; searching non-dwarf plants with anthers, which break linkage separation, from the blue dwarf colony of the main colony A region, sorting blue grain plants, and establishing the double colony C region in autumn sowing;

white seeds sorted by the blue dwarf-male-sterile plants are planted and selfed for one to two generations in a mixed mode according to a community in the main community B, then single spikes and seed spike rows are selected in the community, and part of excellent seed selection seeds are mixed and used as the upper generation male parent of the next round of the next generation of the blue dwarf-male-sterile plants to be planted on one side of the round of the blue dwarf-male-sterile plants;

and the double-group C region is used for selecting a seed nursery for blue wheat. The seeds of the wheat seed are respectively from blue-bred plants in the main group A region, male parent row seeds in the auxiliary group D region, full blue selection ears in the auxiliary group E region and blue wheat ear row and ear system seeds of the double group C region, so that male parents are provided for the auxiliary group D region of the so-called blue dwarf-male-sterile blue wheat, which is constructed later; the double-group C region is a blue wheat Quan Lanshan spike row and spike line planting, a blue wheat seed selection garden with stable subculture and a blue wheat spike line yield comparison test seed selection region;

the secondary group D region is from the main group A region to the main group A region, and the father parent is from the excellent full blue selection spike, spike row or spike line selected in the double group C region in the previous round. And the parent line seeds of the wheat seeds enter the seed spike line of the double-group C area again from the selected single spike, or the parent line seeds are mixed in the auxiliary group E area or the double-group C area to be mechanically sown into the cell selection single spike and then enter the seed spike line of the double-group C area, so that the subsequent blue wheat variety breeding is performed.

And the seed in the secondary group E is from blue seed of the secondary group D, which is obtained by blue dwarf and male pollination, mechanical sowing in a district is adopted for selfing for two generations so as to ensure that blue grain shape is homozygous and stable, full blue grain selection is selected in the second selfing generation, and then, the blue wheat variety is bred by planting spike rows in the double group C.

Further, the running mode of the blue dwarf male-sterile double-group five-nursery area is as follows:

selecting a blue dwarf single plant in the offspring of the main group A region, and sorting blue grains to provide blue dwarf for the main group A region and the auxiliary group D region; the main group B region provides male parent for the main group A region, and white grain varieties are bred; the secondary group D region enriches blue wheat genetic basis and provides basic germplasm resources for blue wheat seed selection for the secondary group E region and the double group C region; the secondary group E area bears the responsibility of selecting ears by selfing and expanding the seeds of the secondary group D area; the double-group C region provides male parent for the auxiliary group of blue dwarf-male-sterile wheat, and performs yield comparison, stability observation and identification and blue wheat variety breeding on the spike row and spike line planting of blue wheat from the main group A region, the auxiliary group D region, the auxiliary group E region and the double-group C region.

Compared with the prior art, the beneficial effects of the method are as follows:

1. by establishing and running the blue dwarf-male-sterile main-auxiliary double-round selection group, especially the design of the blue dwarf-male-sterile auxiliary-round selection group, the non-dwarf fertile plants which are only selected to break the linkage in the blue dwarf-male-sterile group (the main group A area) are broken away from the limitation of low probability of breaking the linkage and few types, and by establishing the blue dwarf-male-sterile auxiliary-round selection group, all genetic types of the main group can be reserved in the blue wheat variety types, the blue wheat germplasm types are enriched, and the opportunity of breeding breakthrough blue wheat varieties is increased.

2. After the secondary group seeds are planted in the secondary group E district for two continuous generations of selfing, a large number of homozygous blue individuals can appear in the group through gene recombination, and a large number of homozygous stable individuals can also appear in other characters.

Detailed Description

The following description of the technical solutions in the embodiments of the present invention will be clear and complete, and it is obvious that the described embodiments are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.

Blue dwarf-male-female and group varieties:

group male parent: the wheat is characterized in that the wheat is selected from the group consisting of Jimai 22, taishan 23, black horse No. 1, black horse No. 2, lu Mai, lu mai 21, shannon 20, jimai 20, yan nong 19 and wheat No. 8, 10 main wheat varieties examined and approved in Shandong province are mixed and planted according to a specific proportion to be pollinated male parent, a wheat 'blue dwarf male-sterile' Shandong round selection group is built, the wheat is planted in an independent isolation area together, any male parent is 'blue dwarf male-sterile' to pollinate freely, and the mature blue dwarf male-sterile and male parent which pollinate firmly are harvested and threshed respectively and collected separately.

Group blue dwarf-male-female: the number of the group blue dwarf-male-sterile is proper and better, and the genetic basis of the round selection group can be widened, but the embodiment is limited by the resources at the time, and only a group of blue dwarf-male-sterile black horse No. 1 backcross group is utilized, which is formed by continuously backcross breeding of blue dwarf-male-sterile 97-866 provided by Sichuan Wenchang agricultural science by Zibo-loving min-seed industry limited company with black horse No. one as a backcross male parent.

Example 1

1. Main group establishment and operation

1. Primary colonisation of main group a region

Sowing 6 rows of blue dwarf male-sterile black horses, wherein the row spacing is 20 cm, sowing single seeds, sowing 25 seeds/m, sowing 1 row of uniformly mixed male parent on each side (in order to dilute the proportion of the blue dwarf male-sterile black horses in the genetic background, the black male-sterile black horses are removed from the male parent group), and sowing the male parent by adopting a wide seedling zone of about 100 seeds/m. Small walkways with the width of 1 meter are reserved at the periphery. And (3) in the growing period, removing the mixed plants and the separated anther plants in the blue dwarf male parent population in time, and removing various obvious mixed plants in the male parent population. Before heading, building a partition wall by using bamboo piles, iron wires and plastic woven cloth, wherein the height of the wall is about 1.2 meters, and building a partition area.

Free pollination is carried out in the flowering stage, and the small bamboo poles are used for poking the male parent plants for supplementary pollination in the pollination full stage so as to increase the setting rate. Harvesting the blue dwarf male parent and the blue dwarf male parent respectively in time after maturation, threshing respectively, and storing respectively. The blue dwarf-male-sterile pollinated fruits are sorted according to the grain colors, blue grains account for about 1/4 and white grains account for about 3/4, recurrent groups for the next generation are respectively stored, the sorted blue grains are used for the blue dwarf-male-sterile of the first recurrent, one part of the sorted white grains are used for the male parent of the recurrent group, and the other part of the sorted white grains are used for establishing recurrent male parent seed selection gardens (main group B area).

2. Primary group a zone first round selection

The field layout is the same as that of the initial colonisation, 6 rows of sorted blue grains (blue dwarf male parent) are sown, 1 row of male parent is sown on two sides (one side is sown with the white grains of the blue dwarf male parent, the other side is still mixed with the group parents to form the male parent, so that the black horse number I is removed from the group male parent due to the proportion of the blue dwarf male parent in the genetic background), and walkways are arranged around.

An isolation area is built before heading, various anther plants separated from the blue dwarf-male-sterile plants are removed, natural pollination or artificial supplementary pollination is carried out in the isolation area, bad single plants are eliminated in the blue dwarf-male-sterile groups, good single plants are selected and reserved, threshing is carried out on the collected and reserved blue dwarf-male-sterile plants, the blue grains are sorted according to the grain colors, the blue grains are sorted as the blue dwarf-male-sterile of the next round, a part of the sorted white grains are used as male parents of a main group, and a part of the white grains enter a recurrent male parent seed selection nursery (main group B area) for planting and selection.

3. Establishment of main group B area recurrent selection main group seed selection nursery

White grains obtained by sorting seeds generated by group blue dwarf and male pollinating are planted in a thin sowing mode in a community, a large number of spikes are selected, part of spikes are selected, mixed and threshed to serve as male parents (upper-generation male parents) of the next round, and part of spikes are selected to plant spike rows.

4. Second round selection of main group A area

The layout is still the same as that of the previous round, 6 rows of blue dwarf male parent (blue grains sorted in the first round) are sown, 1 row of male parent is sown on each side (one side is the current-generation male parent: white grains sorted in the current year; the other side is the previous-generation male parent: mixed-selection mixed-removal single spike seeds from a round selection main group seed selection garden B area), and walkways are arranged around. The isolation region is built before heading, various anther plants separated from the blue dwarf-male-sterile plants are pulled out, natural pollination or artificial supplementary pollination is carried out in the isolation region, bad single plants are eliminated in the blue dwarf-male-sterile population, good single plants are selected and reserved, the first rounds pay attention to more selection and less elimination, and the genetic diversity of the population is reserved as much as possible. Threshing the harvested and selected blue dwarf-male-female, sorting the blue grains as the blue dwarf-male-female of the next round, taking part of the sorted white grains as the male parent (the current-generation male parent) of the main group, and taking part of the white grains as the seed selection nursery (the main group B area) of the main group for planting and selecting.

5. Primary group B zone first round selection

And (3) carrying out cell thin sowing on the white seeds sorted in the current year, and planting the selected spikes of the last turn selected in summer as spike rows. The current turn selects single spike from the annual thin sowing district, most of the single spike is reserved as seed spike row, and part of the single spike is used as the parent of the previous generation; the spike row is preferred from the spike row of the previous round, and partial seeds are taken from each row in a plurality of selected spike rows and mixed into the parent of the previous generation of the round selection group. The excellent spike is promoted to be the spike system, the spike system comparison is carried out, the second spike selection of the protruding spike system is carried out, and the spike system is planted again.

6. Operation of blue dwarf-male-sterile selection population main group

In the main group A area, 6 rows of blue dwarf male parent and 2 rows of male parent (1 row of upper generation male parent and 1 row of current generation male parent) are planted alternately, and are planted in an independent courtyard or an isolation area of a relatively higher isolation wall, and a district pavement is not reserved between two blue dwarf male parent planting belts. All anther plants separated from the dwarf and the dwarf are removed in time or bagged for other use before heading, free pollination or artificial supplementary pollination is carried out in the flowering pollination period, the dwarf and the dwarf plants with poor expression are eliminated after maturation, harvesting blue dwarf-male-sterile plants, threshing and sorting according to the grain colors, wherein one part of blue grains are continuously sown in the area A as the blue dwarf-male-sterile plants of the next round, and the other part of blue grains are used for blue dwarf-male-sterile wheat of the blue wheat alternate-selection group; and (3) selecting white grains, wherein one part of the white grains are directly used for contemporary male parents of main groups of the dwarf-male-sterile alternate selection group, and the other part of the white grains are used for planting selection spikes in cells of a B region of the main group.

In the main group B area, the sorted white grains are subjected to district planting selfing, ears are selected before maturation, one part of the sorted ears are mixed and threshed and one part of the ear row seeds of the previous round are used for the upper generation male parent of the main group, and the other part of the sorted ears are planted for ear rows, so that the upper generation male parent is provided for the next round of main group A area round selection group. This is chosen round by round. And carrying out variety breeding work on particularly excellent spike rows according to a pedigree seed selection program.

The parent group in the main group A region can be treated as a part similar to the main group B region, the ears are selected from the parent before maturity, and the parent group directly enters the ear row planted in the main group B region, or the ear row is selected and threshed in a mixed mode to be used as a part of the parent of the upper generation.

The first two rounds are that the number of basic seeds in the early stage of the group is small, and in order to enlarge the group requirement, the blue dwarf-male is generally only pulled out of individual bad single plants, most of blue dwarf-male plants are reserved, the blue seed sowing is carried out, the group size is enlarged, 6 rows of blue dwarf-male parent and 1 row of pollination male parent are adopted in the field layout of the large-scale group, the small walkways are not reserved in the field, large-furrow planting or planting is carried out in a courtyard, or relatively firm isolation walls with the height of more than 1.5 meters are built around the group. The male parent selection planting area for sorting white grains can be planted in the same large isolation area, then the isolation area which is slightly shorter (1.2 meters can be built), and the male parent selection nursery isolation area can be independently built at alternative places.

2. Establishment of double group C region

After repeated selection, the overall character level of the wheat Lan Ai abortive round-selection group is greatly improved, and the non-dwarf fertile plants which break the linkage and appear in the blue dwarf are searched from the blue dwarf round-selection group in the main group A area, bagging and hanging, after the pollination of the group is finished, the bagging is removed, the plastic rope is used for loose bagging, hanging is checked, and related information is marked in detail. And (3) in the mature period, pulling and collecting the plants one by one according to the selected plants, threshing the plants, and sorting the blue grains of the plants to be planted.

According to the selected plants, the blue grains are sowed and sorted one by one, the single grains are sowed, the grain is 20 grains/m, each single plant is sowed in double rows, and the row length is usually 2-3 m. And checking the color of the seeds one by one during harvesting, wherein part of the single plants have blue and white seeds, and all the seeds of part of the single plants are full blue single plants with blue seeds. Selecting and reserving all blue single plants, carrying out yield screening on plant rows (usually 3 row sowing areas) of the next year of all blue single plants selected, selecting single spikes and spike building rows for the still-separated plant lines, carrying out subsequent breeding according to a systematic seed selection program, and simultaneously carrying out yield comparison on each plant line until the plant lines are stable and breeding blue wheat.

And mixing the single plants with the particle-color separation, sorting blue particles of the single plants, planting in a community, and selecting full blue spikes from the single plants before maturation. The spike row of the next generation is planted, and can be combined into the spike row of the whole blue spike system of the previous generation for uniform comparison and selection.

The whole blue single spike selected in the subsidiary group E region is planted in the C region to participate in the blue wheat variety breeding system. The blue plant from the main group A region and the whole blue spike from the auxiliary group E region are planted in the C region, and participate in blue wheat variety breeding and provide male parent for the auxiliary group D region, so the C region is called as a double group C region.

After several runs, the blue-cultivated plants in main group a are generally not bagged and usually removed as hybrid plants unless they are particularly good, while the seeds in region C are mainly all blue rows from all blue selection plants in region E. Yet another important source is the row of ears from the full blue ear selection planting of the parent row of the D zone of the minor group.

Before sowing, uniformly classifying and numbering seed sources from each region, and making a planting plan: the plant spike row from the upgrade of the main group A region in the C region, the full blue spike row from the father of the auxiliary group D region and the full blue spike row from the selfing expansion of the seed cell of the blue dwarf-male of the auxiliary group E region are registered clearly, and the planting quantity and the area are planned. The spike lines corresponding to each turn (or year) are also planned with the planting quantity and area one by one. And planning and designing the spike yield comparison test. The spike stability is synchronously observed while the yield is compared, and the system breeding procedure is followed until a stable blue wheat variety or strain is developed.

In the process of breeding blue wheat variety, the double-group C region gathers excellent blue wheat selection, spike row and spike line, provides male parent for blue dwarf male group in turn by roll, and keeps the genetic diversity of male parent.

3. Creation of a secondary group D zone

Blue grains selected by each blue dwarf single plant selected by each round of blue dwarf-male-sterile group primary group are used as blue dwarf male-sterile lines of blue wheat secondary group primary group A, a plurality of excellent blue wheat selection ears, ear rows and ear lines are selected from a blue wheat seed selection garden (double group C area) and are mixed to be used as pollination male parents of the blue dwarf-male-sterile blue wheat secondary group, so that a dynamic blue dwarf-male-sterile blue wheat seed selection group (secondary group) is built, and seeds generated by blue dwarf-male-sterile pollination directly enter a secondary group seed planting area, namely a secondary group seed selfing expansion group garden (E area).

The specific operation procedure is as follows: and (3) taking part of blue grains sorted in the main group A area as blue dwarf male-sterile seeds of the auxiliary group, sowing 6 rows, 20 cm in row spacing and 25 grains/m in plant spacing, sowing all blue grain seeds from the C area on two sides as male parents, building an isolation area before heading, not pulling out blue-sterile plants in the blue dwarf male-sterile seeds, manually performing supplementary pollination, respectively harvesting seeds generated by the blue dwarf male-sterile pollination after maturation, and directly entering the E area for sowing and planting.

Treatment of minor group D region male parent: the male parent of the secondary group D is excellent full blue spike selection, spike row or spike line selected from the previous round, the process of taking the male parent as the male parent is also the self-copulation stable process, the full blue single spike selected from the male parent is selected, and then the spike row and spike line are planted in the double group C for comparative selection, and the sub-copulation is further stable. Sowing other seeds in the secondary group D region, and also sowing in all the cells in the E region (or the C region), carrying out selfing again for secondary group expansion, selecting all blue single ears from the seeds, and then carrying out blue wheat variety breeding procedures in the seed rows and the ear lines of the double group C region.

The blue dwarf male parent of the secondary group is the blue dwarf male parent from the harvesting and sorting of the corresponding main group in the current year, and the pollinated male parent of the secondary group is all the preferred blue wheat strains from the blue wheat variety planting seed selection nursery (double group C area) as male parent, and the character level of the secondary group is more and more optimal in turn along with the round selection of the blue dwarf male parent of the main group, and the character level of the parent is also better along with the selection of the blue wheat seed selection nursery, so that the overall character level of the blue dwarf male-sterile blue wheat wheel selection secondary group can be improved in turn.

4. Creation of a secondary group E zone

The seeds (almost all blue grains) from the blue dwarf and abortive pollination of the sub-group D are directly planted in the district, a district seeder is generally adopted for mechanical sowing, the conventional field management and the normal field investigation record, the total area real harvest and yield counting are carried out, and the yield is counted. Whether the seeds are subjected to color sorting or not, a small part of blue grains and white grains still exist in the seeds, and only the proportion of the blue grains is different. The next generation seeds are sorted into blue grains or not, the planting area of a community is enlarged (secondary selfing and expanding), a community seeder is adopted for mechanical sowing, conventional field management and investigation record are adopted, excellent all blue grain selection is selected before maturation, the all blue grain selection is carried out, single spike threshing is carried out for preservation, the seeds are planted into blue wheat seed selection nursery planting spike rows in a double-group C area, and blue wheat varieties are selected according to a systematic seed selection program. Part of the seeds of the good blue wheat strain are used as male parent of the blue dwarf-male-sterile blue wheat secondary alternate selection group in the secondary group D region of the next round.

5. Overall layout and field arrangement

Before sowing, five nursery areas of the main and auxiliary double-wheel clusters of the dwarf male-sterile are planted, and field arrangement is finished according to the characteristic planting modes of each planting area, whether an isolation area needs to be independently established or not and other overall layout. The main group A region needs to establish a very strict isolation region; the main group B area needs to be isolated from any blue wheat area so as not to be polluted by pollen; the double-group C area and the E area are blue wheat planting and seed selecting areas, can be adjacently planted in the same isolation area, and can share one isolation area with the auxiliary group D area of the blue dwarf-male-sterile blue wheat auxiliary group.

After the overall layout, the reasonable planning is made according to the number of seeds selected and reserved in each nursery area in the current year and the scale of the planting area. The operation mode is as follows:

selecting a blue dwarf single plant in the primary group A region, and sorting blue grains to provide blue dwarf for the group A and the secondary group D region; the main group B region provides male parent for the main group A region, and white grain varieties are bred; the secondary group D region enriches the blue wheat genetic basis and provides basic germplasm resources for blue wheat seed selection for the secondary group E region and the double group C region; the secondary group E area bears the responsibility of selecting ears by selfing and expanding group of seeds in the D area; the double-group C region bears the task of providing male parent for the subsidiary group of blue dwarf-male-sterile wheat, and more particularly, the cultivation of spike rows and spike lines of blue wheat from A, D, E and self C region, yield comparison, stability observation and identification and the mission of blue wheat variety breeding are carried out.

6. Source of the germplasm material used

Jimai 22: the Shandong province agricultural academy of sciences crop institute. In 2006, the approval numbers are determined by the Shandong province and the country approval at the same time: lu Nong No. 2006050 and No. 2006018. The Zibo lover seed industry limited company is collected and provided.

Black horse No. 1: the Shandong state black horse seed industry Limited company is bred, the Shandong province approval in 2004, and the approval number (Lu Nong audit word 2004 No. 026. Zibo loving people seed industry Limited company is collected and provided).

Black horse No. 2: the Shandong state black horse seed industry limited company is bred, the Shandong province approval in 2006 is Lu Nong to 2006055, and the Zibo lover seed industry limited company is collected and provided.

wheat 8: the breeding of the academy of agricultural science in the city of Fangfang is carried out by approval in Shandong province in 2003, and the approval number is: lu Nong the word is examined [2003] 028. The Zibo lover seed industry limited company is collected and provided.

Jimai 20: the Shandong province institute of agricultural science developed, 8 months in 2003 passed the Shandong province approval, approval number: lu Nong the word [2003] 029. The Zibo lover seed industry limited company is collected and provided.

Lu Mai 23: the Shandong black horse seed industry limited company and the victory oilfield horse mill farm cooperate and select a seed, and in 1996, the Shandong province approval is passed, and the approval number is the Lu seed audit number 0201. The Zibo lover seed industry limited company is collected and provided.

Lu Mai 21: and (3) breeding by using a tobacco stand city academy of agricultural sciences, and in 1996, checking the tobacco stand city of Shandong province, wherein the checking number is Lu-seed checking number 0199. The Zibo lover seed industry limited company is collected and provided.

Tobacco grower No. 19: the breeding of the agricultural sciences of the tobacco stand city is examined and approved in Shandong province in 2001, the examined and approved number is Lu Nong examined word No. 2001, and the Zibo lover seed industry Limited company is collected and provided.

Shannong 20: breeding at Shandong agricultural university, passing national Huang-Huai nan-piece approval in 2010, wherein the approval number is national audit wheat 2010006. The approval number of 2011 is national audit wheat 2011012 through national Huang-Huai-Bei tablet approval. The Zibo lover seed industry limited company is collected and provided.

Taishan No. 23: the Taian national academy of sciences was bred, and in 2004, was approved by Shandong province, and Lu Nong was reviewed in [2004] 023. The Zibo lover seed industry limited company is collected and provided.

The blue dwarf male-sterile black horse No. 1 backcross group is formed by continuously backcross breeding blue dwarf male-sterile 97-866 provided by Sichuan Wenchang agricultural science by using black horse No. 1 as a backcross male parent of a carrier loving min seed industry limited company, and the carrier loving min seed industry limited company is collected and provided.

The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.

Claims (8)

1. A method for breeding blue wheat variety by blue dwarf male-sterile main and auxiliary double-round group selection is characterized by comprising the following steps:

through the design of the auxiliary round selection group of the blue dwarf-male-sterile blue wheat, a technical system for breeding blue wheat varieties by utilizing the correlated double-group five-nursery region is formed;

the double-group five-nursery area comprises a main group A area, a main group B area, a double-group C area, a secondary group D area and a secondary group E area; the blue grains separated by the blue dwarf male sterile line of the main group blue dwarf male sterile line and the secondary group blue dwarf male sterile line are used for each round, and the two-group character level is synchronously improved for each round.

2. The method for breeding blue wheat varieties by utilizing blue dwarf-male-sterile main and auxiliary double-round group selection, which is characterized in that the main group A area comprises a blue dwarf-male-sterile main group planting seed selection garden; the main group B region comprises a main wheel group wheat variety selection nursery; the double-group C region comprises seed selection gardens from the main group A region, the double-group C region, the auxiliary group D region, the auxiliary group E region and 4 blue wheat regions; wherein the secondary group D region is a male parent; the secondary group D region comprises a blue dwarf-male-sterile blue wheat secondary group selection planting garden; the secondary group E region comprises a secondary group seed selfing and expanding group nursery.

3. The method for breeding blue wheat varieties by using blue dwarf-male-sterile main and auxiliary double round group selection according to claim 2, wherein the main group A region, the main group B region, the double group C region, the auxiliary group D region and the auxiliary group E region respectively comprise respective isolation regions.

4. The method for breeding blue wheat varieties by utilizing blue dwarf-male-sterile main and auxiliary double round group selection according to claim 3, wherein the main group B area needs to be isolated from any blue wheat area;

the double-group C region and the auxiliary group E region are blue wheat planting regions which can be adjacently planted in the same isolation region, and can share one isolation region with the auxiliary group D region of the blue wheat alternate auxiliary group.

5. The method for breeding blue wheat varieties by utilizing blue dwarf-male-sterile main and auxiliary double round selection groups according to claim 3 or 4, wherein the main group A region takes blue dwarf-male-sterile as a sterile line, takes common white wheat varieties as male parents to form a blue dwarf-male-sterile round selection group main group, selects blue dwarf-male-sterile, and provides blue dwarf-male-sterile for the main and auxiliary double round selection groups round by round;

the main group B region is based on white grains of main group blue dwarf-male-sterile pollination, seed selection is carried out for the main group and male parents are provided for the main group in turn, and the major line material is selected and bred into a new variety of common white grain wheat;

the double-group C region carries out planting selection on blue wheat basic materials from all groups except the main group B region, breeds blue wheat varieties, and provides male parent for blue dwarf-male-sterile blue wheat auxiliary groups in turn;

the secondary group D area updates the blue dwarf male parent and the blue dwarf female parent in turn to generate blue wheat seed selection germplasm resources with wide genetic basis and rich types;

and sowing the seed cells provided by the auxiliary group E area, carrying out continuous two-generation selfing group expansion, selecting full blue single ears, and sending the planting ear rows of the double group C area to carry out subsequent breeding to obtain blue wheat varieties.

6. The method for breeding blue wheat varieties by using blue dwarf-male double round selection according to claim 5, wherein the blue selection in the secondary group E region and the blue selection from the male parent in the secondary group D region are both fed into the row of the double group C region, because the blue plants from the primary group A region and the blue selection from the secondary group E region and the blue selection from the secondary group D region are planted in the double group C region.

7. The method for breeding blue wheat variety by utilizing blue dwarf male-sterile main and auxiliary double round group selection according to claim 6, wherein the operation method of the main group A area is as follows: according to the recurrent selection method, selecting the blue dwarf single plants by each recurrent generation of the recurrent group, sowing male parents on two sides of a blue dwarf sterile line, forming two kinds of grains with different colors of blue grains and white grains on the same wheat ear after blue dwarf pollination, carrying out grain color sorting on the blue dwarf single plants, and sorting the blue grains as the blue dwarf of the next recurrent; searching non-dwarf plants with anthers, which break linkage separation, from the blue dwarf colony of the main colony A region, sorting blue grain plants, and establishing the double colony C region in autumn sowing;

white seeds sorted by the blue dwarf-male-sterile plants are planted and selfed for one to two generations in a mixed mode according to a community in the main community B, then single spikes and seed spike rows are selected in the community, and part of excellent seed selection seeds are mixed and used as the upper generation male parent of the next round of the next generation of the blue dwarf-male-sterile plants to be planted on one side of the round of the blue dwarf-male-sterile plants;

and the double-group C region is used for selecting a seed nursery for blue wheat. The seeds of the wheat seed are respectively from blue-bred plants in the main group A region, male parent row seeds in the auxiliary group D region, full blue selection ears in the auxiliary group E region and blue wheat ear row and ear system seeds of the double group C region, so that male parents are provided for the auxiliary group D region of the so-called blue dwarf-male-sterile blue wheat, which is constructed later; the double-group C region is a blue wheat Quan Lanshan spike row and spike line planting, a blue wheat seed selection garden with stable subculture and a blue wheat spike line yield comparison test seed selection region;

the secondary group D region is from the main group A region to the main group A region, and the father parent is from the excellent full blue selection spike, spike row or spike line selected in the double group C region in the previous round. And the parent line seeds of the wheat seeds enter the seed spike line of the double-group C area again from the selected single spike, or the parent line seeds are mixed in the auxiliary group E area or the double-group C area to be mechanically sown into the cell selection single spike and then enter the seed spike line of the double-group C area, so that the subsequent blue wheat variety breeding is performed.

And the seed in the secondary group E is from blue seed of the secondary group D, which is obtained by blue dwarf and male pollination, mechanical sowing in a district is adopted for selfing for two generations so as to ensure that blue grain shape is homozygous and stable, full blue grain selection is selected in the second selfing generation, and then, the blue wheat variety is bred by planting spike rows in the double group C.

8. The method for breeding blue wheat varieties by utilizing blue dwarf-male-sterile main and auxiliary double-round group selection, which is characterized in that the operation mode of the blue dwarf-male-sterile double-group five-nursery area is as follows:

selecting a blue dwarf single plant in the offspring of the main group A region, and sorting blue grains to provide blue dwarf for the main group A region and the auxiliary group D region; the main group B region provides male parent for the main group A region, and white grain varieties are bred; the secondary group D region enriches blue wheat genetic basis and provides basic germplasm resources for blue wheat seed selection for the secondary group E region and the double group C region; the secondary group E area bears the responsibility of selecting ears by selfing and expanding the seeds of the secondary group D area; the double-group C region provides male parent for the auxiliary group of blue dwarf-male-sterile wheat, and performs yield comparison, stability observation and identification and blue wheat variety breeding on the spike row and spike line planting of blue wheat from the main group A region, the auxiliary group D region, the auxiliary group E region and the double-group C region.