CN117357585A

CN117357585A - Processing method of traditional Chinese medicine rehmannia

Info

Publication number: CN117357585A
Application number: CN202311674422.2A
Authority: CN
Inventors: 蒋以号; 罗远方; 胡峥; 梁磊; 周熹如; 喻志标
Original assignee: Nanchang University
Current assignee: Nanchang University
Priority date: 2023-12-08
Filing date: 2023-12-08
Publication date: 2024-01-09
Anticipated expiration: 2043-12-08
Also published as: CN117357585B

Abstract

The invention provides a processing method of a traditional Chinese medicine rehmannia root, belonging to the technical field of traditional Chinese medicine processing and traditional Chinese medicine products. The technology of the invention takes ginger, dried orange peel and agilawood as auxiliary materials to jointly process rehmannia root, and combines the functions of strengthening spleen and stimulating appetite, drying, warming and resolving phlegm with the dynamic and static combination of prepared rehmannia root to inhibit the greasy stagnation property of prepared rehmannia root so as to dredge the qi stagnation and excessive phlegm of prepared rehmannia root; the agilawood is utilized to restore qi and enter kidney, and the guiding drug is utilized to restore the function of dantian, so that the prepared rehmannia root can be used for restoring kidney, nourishing kidney yin and tonifying kidney essence; it is indicated for diarrhea due to spleen and stomach deficiency, qi stagnation and excessive phlegm. The process can expand the medicinal range and the medicinal effect of the prepared rehmannia root, improve the flavor and taste of the prepared rehmannia root, and is simple to operate and suitable for industrial production.

Description

Processing method of traditional Chinese medicine rehmannia

Technical Field

The invention belongs to the technical field of traditional Chinese medicine processing and traditional Chinese medicine products, and particularly relates to a processing method of traditional Chinese medicine rehmannia.

Background

Rehmannia glutinosa (Rehmanniae Radix) is a Chinese medicine for both medicine and food. Rehmannia is taken as a medicinal material and is firstly received in Shennong herbal meridian, is taken as an upper product, is a Henan dao medicinal material and is called as a first essence replenishing medicine; rehmannia root, chinese yam, chrysanthemum and achyranthes root are all four Chinese medicine, and have long history of Chinese medicine.

The traditional Chinese medicine rehmannia root is prepared from radix rehmanniae and prepared rehmannia root by stewing or steaming the radix rehmanniae with wine. Sheng Di Huang is cold in nature and sweet in flavor, and has the actions of clearing heat and cooling blood, nourishing yin and promoting fluid production. The radix rehmanniae is processed into the prepared rehmannia root by steaming and sun drying and other processes, and the prepared rehmannia root is sweet and warm in taste and has the effects of nourishing yin and supplementing blood, and replenishing essence and marrow; but prepared rehmannia root, radix rehmanniae Preparata, sticky and greasy in nature, can prevent digestion, and is contraindicated for patients with excessive phlegm due to qi stagnation, excessive dampness, excessive fullness in middle-jiao, anorexia and loose stool.

The existing processing method of rehmannia is mainly steaming, and the processing process usually uses yellow wine, fructus amomi, ginger and the like as auxiliary materials, for example, publication No. CN101518590A discloses a preparation method of nine-steaming and nine-sun-drying prepared rehmannia, which comprises repeatedly steaming and sun-drying dried rehmannia which is the dried yellow wine for eight times, and mixing fructus amomi powder for the ninth time, steaming and sun-drying; as publication number CN103349706a discloses a method for processing traditional Chinese medicine of prepared rehmannia root, which adds various hypoglycemic components when processing prepared rehmannia root; the publication No. CN102240340A discloses a preparation method of radix rehmanniae Preparata, which comprises soaking radix rehmanniae, draining, adding water, braising, adding fructus Amomi and pericarpium Citri Tangerinae, braising, and steaming with yellow wine. However, the processes all cause the loss of active ingredients in rehmannia root to different degrees, and the prepared rehmannia root is sweet and greasy in taste, is easy to promote wetting and fullness, and is difficult to digest and absorb in spleen and stomach, and the prepared rehmannia root still has reactions such as poor appetite, abdominal distention, loose stool and the like; meanwhile, the processing technology is complex, the production period is long, and the method is not suitable for industrial production.

Disclosure of Invention

The process of the invention is a special process for processing rehmannia together by taking ginger, dried orange peel and agilawood as auxiliary materials: the ginger in the process has pungent taste and slightly warm nature, enters lung, spleen and stomach meridians, and has the effects of pungent and dispersing, warming middle energizer and vomiting, warming lung and resolving phlegm. The dried orange peel enters lung and spleen meridians and is Xin Hangwen-way and good at regulating qi movement; has the effects of regulating qi, strengthening spleen and regulating middle warmer, eliminating dampness and resolving phlegm, and is an essential herb for dispersing lung qi, relieving cough and treating phlegm. The agilawood has the effects of promoting qi circulation, relieving pain, warming middle energizer, relieving vomiting, and relieving asthma. The process can expand the medicinal range and the medicinal effect of the prepared rehmannia root, improve the flavor and taste of the prepared rehmannia root, and is simple to operate and suitable for industrial production.

In order to achieve the above purpose, the present invention adopts the following technical scheme:

a processing method of a traditional Chinese medicine rehmannia root comprises the following steps:

step one, clean selection: taking raw rehmannia root medicinal materials, removing impurities and taking medicinal parts for standby;

step two, moistening: adding yellow wine into radix rehmanniae, sealing in container, moistening overnight;

step three, steaming: mixing rhizoma Zingiberis recens powder, pericarpium Citri Tangerinae powder, and lignum Aquilariae Resinatum powder with rehmanniae radix, mixing, wrapping with gauze, and steaming;

step four, drying: taking out and drying after steaming.

Preferably, in the second step, the mass ratio of the radix rehmanniae to the yellow wine is 10:4-6.

Preferably, the mixing total mass of the ginger powder, the dried orange peel powder and the agilawood powder in the step three is 2.5% -5.0% of the mass of the rehmannia root.

Preferably, the mixing mass of the ginger powder, the dried orange peel powder and the agilawood powder in the step three is 1.0% -1.5% of the mass of the rehmannia root.

Preferably, the steaming time in the third step is 4.5-6.5-h, and the internal and external of the rehmannia root are black and dense.

Preferably, the drying temperature in the fourth step is 55-65 ℃.

Prepared rehmannia root, radix rehmanniae Praeparata has the effects of replenishing blood and nourishing yin, and is pure in taste and strong in strength, and is mainly quiet, and long-term taken has the disadvantage of stagnation of stomach and spleen. The invention uses ginger, dried orange peel and agilawood as auxiliary materials to jointly process rehmannia root, and aims to remove scurry, invigorate spleen and promote appetite, dry warm and resolve phlegm by means of the fragrance and dryness of the ginger and the dried orange peel, and the invention combines with the dynamic and static properties of prepared rehmannia root to inhibit the greasy stagnation property of the prepared rehmannia root so as to dredge the qi stagnation and the excessive phlegm of the prepared rehmannia root. The agilawood is utilized to restore qi and enter kidney, and the guiding drug is utilized to restore the function of dantian, so that the prepared rehmannia root can be used for restoring kidney, nourishing kidney yin and tonifying kidney essence; it is indicated for diarrhea due to spleen and stomach deficiency, qi stagnation and excessive phlegm. Simultaneously, the ginger, the dried orange peel and the agilawood are used as processing auxiliary materials, and the unique fragrance of the dried orange peel can be absorbed into the rehmannia root, so that the prepared rehmannia root has the fragrance of the ginger, the dried orange peel and the agilawood, the properties of the prepared rehmannia root are changed, and the flavor and the taste are good; the preparation process can also promote the production of volatile oil and other effective components.

Compared with the prior art, the invention has the beneficial effects that:

1. the invention innovates and improves the basic processing technology of ginger juice stir-frying, has operability after process optimization, obviously shortens processing time, and is suitable for industrial production.

2. The invention takes the contents of the rehmannia root glycoside D and the catalpol as indexes, optimizes the processing technology of the prepared rehmannia root, determines the optimal technological parameters and processing method, improves the properties of the prepared rehmannia root and increases the content of the active ingredients of the prepared rehmannia root.

Drawings

FIG. 1 shows a rehmannia glycoside D standard substance map.

FIG. 2 is a HPLC chart for measuring content of rehmannoside D in processed radix rehmanniae Preparata according to example 1 of the present invention.

Fig. 3 is a catalpol standard pattern.

Fig. 4 is an HPLC diagram for catalpol content determination in processed radix rehmanniae Preparata according to example 1 of the present invention.

Detailed Description

The following description of the embodiments of the present application will be made clearly and fully with reference to the accompanying drawings, in which it is evident that the embodiments described are only some, but not all, of the embodiments of the present application. The following description of at least one exemplary embodiment is merely exemplary in nature and is in no way intended to limit the application, its application, or uses. All other embodiments, which can be made by one of ordinary skill in the art based on the embodiments herein without making any inventive effort, are intended to be within the scope of the present application.

The rehmannia root raw medicinal materials used in the examples and the comparative examples are all produced from Henan Jiaozu as city.

Example 1

1. And (3) cleaning: selecting raw materials, removing impurities, foreign matters and non-medicinal parts, and placing the rest materials in a clean container.

2. Moistening: placing 100 g radix rehmanniae and 50 g yellow wine in 200 mL beaker, sealing the mouth of the beaker with fresh-keeping film, and moistening overnight to make yellow wine completely absorbed into radix rehmanniae.

3. Steaming: mixing rhizoma Zingiberis recens powder, pericarpium Citri Tangerinae powder and lignum Aquilariae Resinatum powder into rehmanniae radix, and mixing at a ratio of 1 g each per 100 g radix rehmanniae radix adjuvant; the rehmannia is wrapped by gauze and placed in a steamer, and the rehmannia is steamed by water to be 5 h until the inside and outside of the rehmannia are black and dense.

4. And (3) drying: taking out the steamed rehmannia root, drying in an oven at 60 ℃, and cutting into thick slices when the dried rehmannia root is eight times.

Sieving with 80 mesh sieve to remove powder, wherein the content of the powder is less than or equal to 3.0%; detecting content of rehmannoside D and catalpol in the prepared rehmannia root.

Example 2

3. Steaming: mixing rhizoma Zingiberis recens powder, pericarpium Citri Tangerinae powder and lignum Aquilariae Resinatum powder with rehmanniae radix, and mixing at a ratio of 1.5 g each per 100 g radix rehmanniae and adjuvant; the rehmannia is wrapped by gauze and placed in a steamer, and water is separated and steamed for 5.5. 5.5 h until the inside and outside of the rehmannia are black and dense.

Example 3

2. Moistening: placing 100 g radix rehmanniae and 40 g yellow wine in 200 mL beaker, sealing the mouth of the beaker with fresh-keeping film, and moistening overnight to allow the yellow wine to be completely absorbed into radix rehmanniae.

3. Steaming: mixing rhizoma Zingiberis recens powder, pericarpium Citri Tangerinae powder and lignum Aquilariae Resinatum powder with rehmanniae radix, and mixing at a ratio of 1.5 g each per 100 g radix rehmanniae and adjuvant; the rehmannia is wrapped by gauze and placed in a steamer, and the rehmannia is steamed by water to be 5 h until the inside and outside of the rehmannia are black and dense.

Comparative example 1

3. Steaming: mixing rhizoma Zingiberis recens powder and lignum Aquilariae Resinatum powder with rehmanniae radix, and mixing at a ratio of 1 g each per 100 g radix rehmanniae radix adjuvant; the rehmannia is wrapped by gauze and placed in a steamer, and the rehmannia is steamed by water to be 5 h until the inside and outside of the rehmannia are black and dense.

Comparative example 2

3. Steaming: mixing rhizoma Zingiberis recens powder and pericarpium Citri Tangerinae powder with rehmanniae radix, and mixing at a ratio of 1 g each per 100 g radix rehmanniae radix adjuvants; the rehmannia is wrapped by gauze and placed in a steamer, and the rehmannia is steamed by water to be 5 h until the inside and outside of the rehmannia are black and dense.

Comparative example 3

3. Steaming: mixing rhizoma Zingiberis recens powder with rehmanniae radix, and mixing at a ratio of 1 g for every 100 g radix rehmanniae; the rehmannia is wrapped by gauze and placed in a steamer, and the rehmannia is steamed by water to be 5 h until the inside and outside of the rehmannia are black and dense.

The content of the rehmannia glycoside D and the catalpol in the prepared rehmannia root sample is measured by an HPLC method, and the measurement results are shown in the table 1, and the results show that the content of the rehmannia glycoside D and the catalpol in the prepared rehmannia root sample obtained by the embodiment of the invention meet the requirements, and the prepared rehmannia root sample has the effects of nourishing yin, enriching blood, regulating immunity and reducing blood sugar. Fig. 1 and 3 are graphs of rehmannia glycoside D and catalpol standard substance respectively. FIGS. 2 and 4 are HPLC diagrams for determining content of rehmannoside D and catalpol in processed radix rehmanniae Preparata of example 1 respectively; peak 1 in fig. 1 and 2 is rehmannia glycoside D, and peak 2 in fig. 3 and 4 is catalpol.

TABLE 1

Preparation of a rehmannia glycoside D standard solution: taking a proper amount of rehmannia glycoside D standard substance, precisely weighing, adding 25% methanol to prepare a solution containing 70 mug of each 1 mL.

Preparation of a test solution of rehmannia glycoside D: cutting the sample into small blocks of about 5 mm, drying under reduced pressure at 80deg.C for 24 h, grinding into coarse powder, sieving with No. three sieve, collecting about 1 g, precisely weighing, placing in conical flask with plug, precisely adding 25% methanol 25 mL, weighing, ultrasonic treating 1 h (power 400W, frequency 50 kHz), cooling, weighing again, supplementing the reduced weight with 25% methanol, shaking, centrifuging at high speed for 10 min, collecting supernatant, filtering, and collecting subsequent filtrate.

Rehmannia root glycoside D chromatographic conditions and system adaptability: hypersil ODS (5 μm,250 mm. Times.4.6 mm), column temperature 30 ℃, flow rate 1.0 mL/min, methanol-0.1% phosphoric acid solution (5:95) as mobile phase; the detection wavelength was 203 nm.

Preparing catalpol standard substance solution: taking appropriate amount of catalpol standard substance, precisely weighing, adding mobile phase to obtain solution containing 50 μg per 1 mL.

Preparing catalpol sample solution: cutting the sample into small blocks of about 5 mm, drying under reduced pressure at 80 ℃ for 24 h, grinding into coarse powder, taking about 0.8 g, precisely weighing, placing into a conical flask with a plug, precisely adding 50 mL of pure methanol, weighing, heating and refluxing to extract 1.5 h, cooling, weighing again, supplementing the lost weight with pure methanol, shaking uniformly, filtering, precisely measuring 10 mL of the subsequent filtrate, concentrating to near dryness, dissolving the residue with a mobile phase, transferring into a 10 mL measuring flask, diluting to scale with the mobile phase, shaking uniformly, filtering, and taking the subsequent filtrate.

Catalpol chromatographic conditions and system adaptability: octadecylsilane chemically bonded silica is used as a filler; methanol-0.1% phosphoric acid solution (1:99) is taken as a mobile phase; the detection wavelength was 210 nm. The theoretical plate number should be not less than 5000 according to catalpol peak calculation.

Assay: precisely sucking 10 μl of rehmanniae radix glycoside D, catalpol standard solution and test solution, respectively, and injecting into liquid chromatograph for measurement.

The content measurement results show that: the prepared rehmannia root of example 1-example 3 has a content of rehmanniae radix glycoside D higher than 0.12% and catalpol content higher than 0.61%, compared with the prepared rehmannia root of comparative example.

Finally, it should be emphasized that the foregoing description is merely illustrative of the preferred embodiments of the invention, and that various changes and modifications can be made by those skilled in the art without departing from the spirit and principles of the invention, and any such modifications, equivalents, improvements, etc. are intended to be included within the scope of the invention.

Claims

1. A processing method of traditional Chinese medicine rehmannia is characterized by comprising the following steps:

step four, drying: taking out and drying after steaming.

2. The method for processing rehmannia root as defined in claim 1, wherein the mass ratio of radix rehmanniae to yellow wine in the second step is 10:4-6.

3. The method according to claim 1, wherein the total mass of ginger powder, dried orange peel powder and linaloe powder added in the third step is 2.5% -5.0% of the rehmannia root.

4. The method according to claim 1, wherein the mixing mass of the ginger powder, the dried orange peel powder and the linalool powder in the third step is 1.0% -1.5% of the rehmannia root.

5. The method according to claim 1, wherein the steaming time in the third step is 4.5-6.5-h.

6. The method according to claim 1, wherein the drying temperature in the fourth step is 55-65deg.C.