CN115040587A - Prepared rehmannia root decoction pieces and refined preparation method thereof - Google Patents
Prepared rehmannia root decoction pieces and refined preparation method thereof Download PDFInfo
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
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- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/15—Preparation or pretreatment of starting material involving mechanical treatment, e.g. chopping up, cutting or grinding
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/17—Preparation or pretreatment of starting material involving drying, e.g. sun-drying or wilting
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Abstract
The invention discloses a prepared rehmannia root decoction piece and a refined preparation method thereof, and the refined preparation method comprises the following steps of cleaning, washing, moistening, steaming, drying, cutting, drying, sterilizing and packaging: and (3) performing irradiation sterilization on the dried prepared rehmannia root, and then quickly performing vacuum packaging to obtain the finished prepared rehmannia root decoction pieces. Compared with the prior art, the method has the advantages that the steamed steaming liquid and the steamed prepared rehmannia root are uniformly stirred in the conventional process, so that the nutrient components (mainly catalpol in the rehmannia root) in the steaming liquid are absorbed by the prepared rehmannia root, the catalpol in the steaming liquid is further dried by the two drying ovens with different temperatures, the loss of the nutrient components is further reduced, and the catalpol content of the prepared rehmannia root decoction piece is more than or equal to 85.13mg g through actual detection ‑1 And the whole preparation process is simple and easy to operate, has high efficiency, stable process and low cost, and is suitable for industrial production.
Description
Technical Field
The invention relates to the field of traditional Chinese medicine preparation, in particular to prepared rehmannia root decoction pieces and a fine preparation method thereof.
Background
Prepared rehmannia root, radix rehmanniae Praeparata, also known as rehmannia: radix rehmanniae and radix rehmanniae Preparata. Latin name: rehmannia glutinosa (Gaetn.) Libosch. ex Fisch. et Mey. Scrophulariaceae, rehmannia, cultivated in various regions at home and abroad, and the roots and stems of the plants are used as medicines. The prepared rehmannia root, a traditional Chinese medicine, is a processed product of rehmannia root, has black and glossy surface, soft and flexible quality, sweet taste and mild nature, and has the effects of nourishing yin and supplementing blood, replenishing vital essence and replenishing marrow and the like. Can be used for treating deficiency of liver-yin and kidney-yin, soreness of waist and knees, hectic fever, night sweat, nocturnal emission, internal heat, diabetes, blood deficiency, sallow complexion, cardiopalmus, menoxenia, giddiness, tinnitus, and premature gray hair.
Radix rehmanniae fumigates to black and moist, and is prepared rehmannia root, which is irregular block-shaped, has different sizes, is completely black inside and outside, and has luster and uneven external surface. Has moist cross section, bright grease-like block in the center, high viscosity, soft texture, slight smell, and sweet taste. It is better to have a big, soft and moist root tuber, and black and glossy interior and exterior through nine times of steaming and sun drying.
The prepared rehmannia root is a general good and is generally not graded. It is better for large, heavy, soft and oily, black cross section, sweet taste. Especially, Huaiqing Dihuang produced in Henan is the best herb of the four great Huai nationalities. The radix rehmanniae Preparata can be prepared by steaming radix rehmanniae Preparata, processing with wine, processing with ginger wine, processing with fructus Amomi, and processing with charcoal of radix rehmanniae Preparata, storing in a drying container, sealing, and standing in a dry place to prevent mold and moth-eating.
In the prior art, the prepared rehmannia root is usually directly decocted into decoction for taking, the use of the active ingredients in the medicinal materials is low, the absorption is poor, the drug effect is poor, and the decoction is needed for taking every time, so that the traditional Chinese medicine is inconvenient and not easy to carry about. Therefore, a new prepared rehmannia root Chinese medicinal decoction piece product which has high utilization rate of active ingredients, good absorption and convenient taking needs to be developed.
Disclosure of Invention
The invention aims to overcome the defects in the prior art and provides a prepared rehmannia root decoction piece and a refined preparation method thereof.
In order to achieve the purpose, the invention is implemented according to the following technical scheme:
a refined preparation method of prepared rehmannia root decoction pieces comprises the following steps:
1) cleaning and selecting: cleaning and selecting fresh rhizome of rehmanniae radix to remove impurities and non-medicinal part;
2) washing the medicine: putting the cleaned and selected radix rehmanniae into a medicine washing machine to clean silt;
3) moistening the drugs: putting the cleaned radix rehmanniae into a soaking pool, moistening for 6-7 hours, taking out, and spraying water once every one hour until the radix rehmanniae is cut and has no dry core;
4) steaming: putting the dried rehmannia root after the medicine moistening into a steamer to steam for 3-4 hours, and tightly closing for 4-6 hours after the steaming is finished to obtain prepared rehmannia root; taking out radix rehmanniae Preparata, mixing with the steam in the steamer, and cooling;
5) and (3) drying: drying the steamed prepared rehmannia root in an oven at 45-55 ℃ for 1-2 hours, and then drying in an oven at 70-75 ℃ for 2-2.5 hours until the prepared rehmannia root is dried to 8 degrees;
6) cutting: slicing the processed radix rehmanniae Preparata;
7) and (3) drying: drying the cut prepared rehmannia root slices in an oven at 70-75 ℃ for 3-4 hours until the water content of the prepared rehmannia root is not more than 15%; taking out and naturally cooling;
8) and (5) sterilizing and packaging: and (3) performing irradiation sterilization on the dried prepared rehmannia root, and then quickly performing vacuum packaging to obtain the finished prepared rehmannia root decoction pieces.
Preferably, in the step 4), the steam pressure in the steamer is 0.08-0.1 Mpa.
Preferably, in the step 6), the prepared rehmannia root is cut into 2-4mm thin pieces.
Preferably, in the step 8), the irradiation sterilization is performed by using 60 Co-gamma rays, and the irradiation dose is 3 kGy.
In addition, the invention also provides prepared rehmannia root decoction pieces prepared by the method.
Compared with the prior art, the method has the advantages that the steamed steaming liquid and the steamed prepared rehmannia root are uniformly stirred in the conventional process, so that the nutrient components (mainly catalpol in the rehmannia root) in the steaming liquid are absorbed by the prepared rehmannia root, and are dried by the two drying ovens with different temperatures, so that the loss of the nutrient components is further reduced, and the actual detection shows that the nutrient-rich health-care product prepared by the method is capable of reducing the loss of the nutrient componentsThe catalpol content of the prepared rehmannia root decoction pieces is more than or equal to 85.13mg g -1 And the whole preparation process is simple and easy to operate, has high efficiency, stable process and low cost, and is suitable for industrial production.
Drawings
FIG. 1 is a chromatogram of catalpol standard.
FIG. 2 is a chromatogram of a fresh rehmannia glutinosa sample.
FIG. 3 is a chromatogram of prepared rehmannia root decoction pieces prepared in example 3 of the present invention.
FIG. 4 is a chromatogram of dried rehmannia glutinosa Libosch.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail with reference to the following embodiments. The specific embodiments described herein are merely illustrative of the invention and do not limit the invention.
Example 1
1) Cleaning and selecting: cleaning and selecting fresh rhizome of rehmannia glutinosa libosch of Henan province, and removing impurities and non-medicinal parts;
2) washing the medicine: putting the cleaned dried rehmannia root into a medicine washing machine to clean silt;
3) moistening the drugs: putting the cleaned radix rehmanniae into a soaking pool, moistening for 6 hours, taking out, and spraying water once every hour until the radix rehmanniae is cut and has no dry core;
4) steaming: putting the moistened radix rehmanniae into a steamer, steaming for 4 hours under the steam pressure of 0.08Mpa, and tightly closing for 6 hours after the steaming is finished to obtain the prepared rehmannia root; taking out radix rehmanniae Preparata, mixing with the steam in the steamer, and cooling;
5) and (3) drying: drying the steamed prepared rehmannia root in a 45 ℃ oven for 2 hours, and then drying in a 70 ℃ oven for 2.5 hours until the prepared rehmannia root is dried to 8 degrees;
6) cutting: cutting the processed radix rehmanniae Preparata into 2mm slices;
7) and (3) drying: drying the cut radix rehmanniae Preparata slices in an oven at 70 deg.C for 4 hr until the water content of radix rehmanniae Preparata is no more than 15%; taking out and naturally cooling;
8) and (5) sterilizing and packaging: and (3) performing irradiation sterilization on the dried prepared rehmannia root by adopting 60 Co-gamma rays, wherein the irradiation dose is 3kGy, and then rapidly performing vacuum packaging to obtain the finished prepared rehmannia root decoction pieces.
When in use, the decoction pieces are soaked in boiled water for 10 to 15 minutes, and 9 to 15g of prepared rehmannia root decoction pieces are added into 300ml of boiled water per 200-.
Example 2
1) Cleaning and selecting: cleaning and selecting fresh rhizome of rehmannia glutinosa libosch of Henan province, and removing impurities and non-medicinal parts;
2) washing the medicine: putting the cleaned dried rehmannia root into a medicine washing machine to clean silt;
3) moistening the drugs: putting the cleaned radix rehmanniae into a soaking pool, moistening for 7 hours, taking out, and spraying water once every hour until the radix rehmanniae is cut and has no dry core;
4) steaming: putting the moistened radix rehmanniae into a steamer, steaming for 3 hours under the steam pressure of 0.1Mpa, and stewing for 4 hours after the steaming is finished to obtain the prepared radix rehmanniae; taking out radix rehmanniae Preparata, mixing with the steam in the steamer, and cooling;
5) and (3) drying: putting the steamed prepared rehmannia root into an oven at 55 ℃ for drying for 1 hour, and then putting the dried prepared rehmannia root into an oven at 75 ℃ for drying for 2 hours until the prepared rehmannia root is dried to 8 degrees;
6) cutting: cutting the processed radix rehmanniae Preparata into 4mm slices;
7) and (3) drying: drying the cut radix rehmanniae Preparata slices in a 75 ℃ oven for 3 hours until the water content of the radix rehmanniae Preparata is not more than 15%; taking out and naturally cooling;
8) and (5) sterilizing and packaging: and (3) performing irradiation sterilization on the dried prepared rehmannia root by adopting 60 Co-gamma rays, wherein the irradiation dose is 3kGy, and then rapidly performing vacuum packaging to obtain the finished prepared rehmannia root decoction pieces.
Example 3
1) Cleaning and selecting: cleaning and selecting fresh rhizome of rehmannia glutinosa libosch of Henan province, and removing impurities and non-medicinal parts;
2) washing the medicine: putting the cleaned and selected radix rehmanniae into a medicine washing machine to clean silt;
3) and (3) moistening: putting the cleaned radix rehmanniae into a soaking pool, moistening for 6.5 hours, taking out, and sprinkling water once every hour until the radix rehmanniae is cut and has no dry core;
4) steaming: putting the moistened radix rehmanniae into a steamer, steaming for 3.5 hours under the steam pressure of 0.09Mpa, and stewing for 5 hours after the steaming is finished to obtain prepared rehmannia root; taking out radix rehmanniae Preparata, mixing with the steam in the steamer, and cooling;
5) and (3) drying: putting the steamed prepared rehmannia root into a 50 ℃ oven to be dried for 1.5 hours, and then putting the dried prepared rehmannia root into a 72 ℃ oven to be dried for 2.2 hours until the prepared rehmannia root is dried to 8 degrees;
6) cutting: cutting the processed radix rehmanniae Preparata into 3mm slices;
7) and (3) drying: drying the cut radix rehmanniae Preparata slices in a 72 deg.C oven for 3.5 hr until the water content of radix rehmanniae Preparata is no more than 15%; taking out and naturally cooling;
8) and (5) sterilizing and packaging: and (3) performing irradiation sterilization on the dried prepared rehmannia root by adopting 60 Co-gamma rays, wherein the irradiation dose is 3kGy, and then rapidly performing vacuum packaging to obtain the finished prepared rehmannia root decoction pieces.
Catalpol is iridoid glycoside compound, is one of main effective components of rehmanniae radix, has molecular weight of 362, and is unstable under acid and alkali conditions. The modern pharmacological research proves that catalpol has the activities of resisting cancer, senile dementia, reducing blood sugar and blood fat, resisting hepatitis virus, alleviating diarrhea, resisting bacteria, diminishing inflammation, promoting urination, relieving spasm, inhibiting capillary permeability and the like, and is expected to be developed into a new medicine. Therefore, the catalpol content is an important index for determining the specific efficacy of the prepared rehmannia root decoction piece, and the catalpol content in the prepared rehmannia root decoction piece is determined as follows.
The reagents for measurement were as follows: catalpol reference substance, batch number MUST-15051225, purchased from Kyormant Biotechnology Ltd, with purity of more than 99.45% determined by HPLC; acetonitrile and methanol are chromatographically pure, water is distilled water, and other reagents are analytically pure.
The catalpol determination method comprises the following steps: by high performance liquid chromatography
Chromatographic conditions are as follows: and (3) chromatographic column: microsorb C18(4.6 mm. times.250 mm, 5 μm), column temperature: 30 ℃, flow rate: 1.0mL min-1, PDA detector, detection wavelength: 203nm, mobile phase: 0.1% phosphoric acid water (a) -acetonitrile (B), gradient elution, elution procedure: 0-5min (2-3% B); 5-20min (3-4% B); 20-25min (4-4% B); 25-30min (4-2% B); 30-40min (2-2% B). Solution preparation: (1) preparation of control solutions: taking a proper amount of catalpol reference substance, precisely weighing, placing in a 50mL measuring flask, adding methanol to dissolve, and fixing volume to scale to obtain reference substance solution containing 0.464mg per mL.
Preparing a test solution: precisely weighing fresh rhizome of Henan cokes as fresh rhizome of radix rehmanniae, directly drying fresh rhizome of radix rehmanniae till the water content is not more than 15%, 2.0g of prepared rehmannia root decoction pieces prepared in example 3 and fresh radix rehmanniae is not more than 2.0g, placing into a conical flask with a plug, precisely adding 50mL of methanol, weighing, ultrasonically extracting for 0.5 h, cooling, weighing again, supplementing the weight loss by methanol, shaking up, filtering, precisely weighing 10mL of subsequent filtrate, concentrating to near dryness, dissolving residue in water, transferring into a 10mL volumetric flask, diluting with water to a scale mark, shaking up, filtering with a 0.45 mu m microporous membrane, and taking subsequent filtrate.
Fig. 1-4 are analysis diagrams under 2.1 chromatographic conditions, wherein fig. 1 is a chromatogram of catalpol standard, fig. 2 is a chromatogram of a fresh rehmannia glutinosa Libosch sample, and fig. 3 is a chromatogram of prepared rehmannia glutinosa Libosch decoction pieces prepared in example 3 of the present invention; FIG. 4 is a chromatogram of dried rehmannia glutinosa Libosch; as can be seen from the fig. 1-4, the catalpol content of the prepared rehmannia root decoction piece is close to that of fresh radix rehmanniae recen, and as shown in the fig. 3, the catalpol content of the prepared rehmannia root decoction piece prepared in the embodiment 3 of the invention is more than or equal to 85.13 mg/g -1 。
The technical solution of the present invention is not limited to the limitations of the above specific embodiments, and all technical modifications made according to the technical solution of the present invention fall within the protection scope of the present invention.
Claims (5)
1. A refined preparation method of prepared rehmannia root decoction pieces is characterized by comprising the following steps:
1) cleaning and selecting: cleaning and selecting fresh rhizome of rehmanniae radix to remove impurities and non-medicinal part;
2) washing the medicine: putting the cleaned and selected radix rehmanniae into a medicine washing machine to clean silt;
3) moistening the drugs: putting the cleaned radix rehmanniae into a soaking pool, moistening for 6-7 hours, taking out, and sprinkling water once every hour until the radix rehmanniae is cut and has no dry core;
4) steaming: putting the dried rehmannia root after the medicine moistening into a steamer to steam for 3-4 hours, and tightly closing for 4-6 hours after the steaming is finished to obtain prepared rehmannia root; taking out radix rehmanniae Preparata, mixing with the steam in the steamer, and cooling;
5) and (3) drying: drying the steamed prepared rehmannia root in an oven at 45-55 ℃ for 1-2 hours, and then drying in an oven at 70-75 ℃ for 2-2.5 hours until the prepared rehmannia root is dried to 8 degrees;
6) cutting: slicing the processed radix rehmanniae Preparata;
7) and (3) drying: drying the cut prepared rehmannia root slices in an oven at 70-75 ℃ for 3-4 hours until the water content of the prepared rehmannia root is not more than 15%; taking out and naturally cooling;
8) and (5) sterilizing and packaging: and (3) performing irradiation sterilization on the dried prepared rehmannia root, and then quickly performing vacuum packaging to obtain the finished prepared rehmannia root decoction pieces.
2. The method for refining prepared rehmannia root decoction pieces according to claim 1, wherein in the step 4), the steam pressure in a steamer is 0.08-0.1 MPa.
3. The method for finely preparing radix rehmanniae Preparata decoction pieces according to claim 1, wherein in the step 6), the radix rehmanniae Preparata is cut into 2-4mm thin pieces.
4. The method for finely preparing radix rehmanniae preparata decoction pieces according to claim 1, wherein in the step 8), the irradiation sterilization is performed by 60 Co-gamma rays, and the irradiation dose is 3 kGy.
5. A prepared rehmannia root decoction piece obtained by the method as claimed in any one of claims 1 to 4.
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Non-Patent Citations (1)
| Title |
|---|
| 马晓嘉: "熟地黄蒸制工艺的研究", 《中国优秀硕士学位论文全文数据库工程科技Ⅰ辑》, no. 2, pages 49 * |
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