CN103099856A - White paeony root processed product and preparation method thereof - Google Patents
White paeony root processed product and preparation method thereof Download PDFInfo
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- CN103099856A CN103099856A CN2013100304534A CN201310030453A CN103099856A CN 103099856 A CN103099856 A CN 103099856A CN 2013100304534 A CN2013100304534 A CN 2013100304534A CN 201310030453 A CN201310030453 A CN 201310030453A CN 103099856 A CN103099856 A CN 103099856A
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Abstract
The invention provides a white paeony root processed product. Each gram of the white paeony root processed product contains no less than 1.6% of paeoniflorin. The invention also provides a preparation method of the white paeony root processed product. The white paeony root processed product is prepared by adopting cleaning, soaking, distilling and decontaminating methods, and loss of paeoniflorin can be reduced, so that the content of the paeoniflorin is not obviously changed and is increased compared with the content of the finished product processed by the traditional method; and taste of the processed finished product is obviously changed compared with the taste of the traditional white paeony root processed product, bitter taste is basically removed, bitter-cold property of natural medicinal materials is lowered, and cell walls of the natural medicinal materials can be broken, so that macromolecules can be changed into micromolecules, and absorption can be greatly facilitated.
Description
Technical field
The invention belongs to drug world, be specifically related to a kind of Radix Paeoniae Alba processed product and preparation method thereof.
Background technology
(formal name used at school: Paeonia sterniana Fletcher in Joum.) also claim Paeonia sterniana Fletcher in Journ., be Ranunculaceae Paeonia plant to Radix Paeoniae Alba.The Radix Paeoniae Alba is had won fame both at home and abroad at the existing long cultivation history of China, and its root is used as medicine.Perennial herb or undershrub, underground part is block or slightly thick; Phyllopodium gives birth to or stem is given birth to, and is large, alternate, pinniform or trifoliolate leaf or drastic crack; Spend large and beautiful, singly be born in branch top or bunchy sometimes, white; Sepal 5, deposit the place; Petal 5-10, but normal in cultigen is polyphyll; Stamen is most; Floral disc ring-type or cup-shaped; Carpel 2-5, centrifugal growth, in the time of as a result, Bian is the Follicle radish, and each has seed number.Be born in the bushes or thick grass in hillside, mountain valley.The ground such as Chinese Anhui, Heilungkiang, Jilin, Liaoning, Hebei, Henan, Shandong, Shanxi, Shaanxi, the Inner Mongol distribute.
The main effect of the Radix Paeoniae Alba is suppressing the hyperactive liver pain relieving, nourishing blood for regulating menstruation, astringing YIN to stop sweating with curing mainly; Be used for having a headache dizzy, hypochondriac pain, stomachache, limb pain twin, blood deficiency and yellow complexion, menoxenia, spontaneous perspiration, night sweat.Chemical composition is that root contains paeoniflorin, paeonol, paeoniflorin, benzoic acid approximately 1.07%, volatile oil, fatty oil, resin, tannin, sugar, starch, phlegmatic temperament, protein, cupreol and triterpenes.The Radix Paeoniae Alba that produce in another kind of Sichuan contains a kind of acidic materials, and staphylococcus aureus is had inhibitory action.
It is as follows that the Radix Paeoniae Alba has following medical value: analgesic activity: can suppress mouse writhing, shout, the hot plate reaction, morphine is suppressed writhing response synergism, and can resist and faint from fear due to pentylenetetrazole; Spasmolysis: intestinal tube and stomach in place motion are had inhibitory action, significantly resist the uterine contraction that oxytocin causes; Liver protection effect: hepatic injury due to carbon tetrachloride, AFB1, D-galactosamine is had obvious protective effect; Effect to cardiovascular system: coronary artery dilator reduces blood pressure.
The concocting method of the Radix Paeoniae Alba is at present: 1, Radix Paeoniae Alba (parched) claims again Radix Paeoniae Alba (processed), fries to little Huang with slow fire with Testa Tritici for Radix Paeoniae Alba sheet, and slightly focal spot, sieve and remove Testa Tritici, the cool person of being used as medicine of drying in the air; 2, Radix Paeoniae Alba has another name called Radix Paeoniae Alba (parched with wine), Radix Paeoniae Alba preparata, for Radix Paeoniae Alba sheet is mixed thoroughly with the yellow wine sprinkle, and the fried dry person of being used as medicine then.Cold alleviation, the effect of invigorating blood circulation strengthens; 3, Radix Paeoniae Alba (processed with vinegar) has another name called vinegar Radix Paeoniae Alba (parched), vinegar Chinese herbaceous peony, for Radix Paeoniae Alba sheet sprays with rice vinegar, with the little stir-fry of the slow fire person of being used as medicine.Relatively hold back the liver pain relieving, nourishing blood and hemoslasis; 4, the burnt Radix Paeoniae Alba has another name called burnt Chinese herbaceous peony, Radix Paeoniae Alba charcoal, and for Radix Paeoniae Alba sheet is fried with high heat to burned black, sustainability is taken out with the clear water most Mars that goes out, and then dries the person of being used as medicine.Relatively hold back the blood hemostasis.
After traditional concocting method was concocted, paeoniflorin content all significantly reduced, and mouthfeel is poor, and bitter in the mouth is unfavorable for that clinical drug uses.
Summary of the invention
The object of the invention is to overcome the deficiencies in the prior art, a kind of Radix Paeoniae Alba processed product and preparation method thereof is provided.
The technical scheme that realizes above-mentioned purpose is: a kind of Radix Paeoniae Alba processed product, it is characterized in that, and contain paeoniflorin in every gram processed product and be not less than 1.6%.
Further preferred, contain paeoniflorin in every gram processed product and be not less than 1.8%.
It comprises the steps:
A, the rhizome of getting the fresh Radix Paeoniae Alba are cleaned, section, make Radix Paeoniae Alba sheet;
B, the Radix Paeoniae Alba sheet that a step is chosen soak, mix with Testa Tritici distillation, drying, redistillation, drying;
C, yeast and distilled water are cultivated, then carried out ferment at constant temperature with the Radix Paeoniae Alba that the b step makes, the Radix Paeoniae Alba removal of impurity after fermentation, drying, and get final product.
Wherein, the described ferment at constant temperature temperature of c step is 25 ℃-28 ℃, and time: 12h-24h, pH value are 5-5.5.
The Radix Paeoniae Alba processed product that adopts concocting method of the present invention to concoct reduces the loss of peoniflorin, and the content that makes peoniflorin increases without significant change and the finished product content concocted than traditional method.The mouthfeel of the more traditional Radix Paeoniae Alba processed product of the finished product of concocting out changes obviously, and bitterness is removed substantially, reduced the property of the bitter cold of crude drug, and can make its cell wall breaking, thereby macromole changes micromolecule into, more is conducive to absorb.
Description of drawings
Fig. 1 is peoniflorin canonical plotting of the present invention.
Fig. 2 is peoniflorin reference substance HPLC chromatogram of the present invention.
Fig. 3 is the ferment HPLC chromatogram of the Radix Paeoniae Alba of the present invention.
Fig. 4 is benzoic acid canonical plotting of the present invention.
The specific embodiment
In order to further illustrate the present invention, below in conjunction with specific embodiments, the present invention is made a more detailed description and the effect explanation, should be noted that, only further illustrating description in this embodiment of listing, and do not mean that the scope of the invention is limited, the present invention is limited by the scope of claims and equivalent thereof.
A kind of Radix Paeoniae Alba processed product disclosed by the invention contains paeoniflorin in every gram processed product and is not less than 1.6%, or contains paeoniflorin in every gram processed product and be not less than 1.8%, contains paeoniflorin in optimum every gram processed product and is not less than 3.3%.
Its step preparation by the following method:
A, the rhizome of getting the fresh Radix Paeoniae Alba are cleaned, section, make Radix Paeoniae Alba sheet;
B, the Radix Paeoniae Alba sheet that a step is chosen soak, mix with Testa Tritici distillation, drying, redistillation, drying;
C, yeast and distilled water are cultivated, then carried out ferment at constant temperature with the Radix Paeoniae Alba that the b step makes, the Radix Paeoniae Alba removal of impurity after fermentation, drying, and get final product.
Wherein, the described ferment at constant temperature temperature of c step is 25 ℃-28 ℃, and time: 12h-24h, pH value are 5-5.5.
The contained chemical composition of the Radix Paeoniae Alba has certain particularity, and the one, Radix Paeoniae Alba index composition peoniflorin is that pinane is derivant, can be become by wherein contained dilute acid hydrolysis volatile pinane derivative and glucose under certain temperature and humidity, and drug effect is reduced; The 2nd, in the Radix Paeoniae Alba, the astringent component tannin can be condensed into the acid anhydride of tanning under the conditions such as diluted acid, high temperature, claims again tannic red.Tannic red is water insoluble, is difficult for friedly, and reduces astriction.Therefore should notice that Radix Paeoniae Alba should not soak for a long time in Radix Paeoniae Alba processing concocting process, otherwise general red stain color reduces drug effect and even loses curative effect, should heat by slow fire in the parch process, anti-firepower is too quickly and make Radix Paeoniae Alba (parched) lose the merit of spleen invigorating, should not be exposed to the sun by high light in dry run.The main Preparation process product of the Radix Paeoniae Alba have Radix Paeoniae Alba decoction pieces, Radix Paeoniae Alba (parched) and Radix Paeoniae Alba etc., and the research of its Preparation process mainly is changed to index with paeoniflorin content, and as the main evaluation foundation of end product quality standard.Whether that the research of the processing in production place of the Radix Paeoniae Alba mainly concentrates on is softening, remove the peel, cooking technology, cutting process, stove drying and take advantage of bright processing technique etc., generally needs 2～4 days, time-consuming.
Fermentation method is one of Chinese medicine processing method always, and along with the development of science and technology, the scope that fermentation method is used is more extensive.This method changes the original property of medicine of Chinese medicine by the effect of microorganism, improves curative effect, reduces toxic and side effects, enlarges indication.Microorganism produces various enzymes in growth course, the composition decomposition and inversion of medicine is decomposed the toxic and side effects that reduces medicine for new active component or with toxic component.And herb fermenting is the biotransformation that carries out under room temperature, condition of normal pressure, has protected to greatest extent the active component of Chinese medicine, as to compositions such as heat sensitive volatile oil and vitamin.Chinese medicine has changed the content of its active component under the effect of microorganism, and make the composition of tunning and material composition different, and the Chinese herbal medicine cell carried out breaking cellular wall make the active substance stripping, thereby improved the concentration of active component.And the macromole effective active matter that microorganism can not directly absorb many human bodies during the fermentation is degraded into micromolecular active substance, and fermented tcm can be absorbed in human body faster, and absorption is more complete, and therapeutic effect is good.
The herb fermenting technology is the focus of R﹠D of modern TCM, but still is in the starting stage of development, and Yang Xiuwei modifies active ingredient of Chinese herbs such as glycoside, flavonoid, Coumarinses with intestinal bacteria; Compare with fungus, antibacterial has larger advantage as zymocyte, and the growth cycle of antibacterial is short. and technique is simple, makes it become new study hotspot in the fermented tcm field.We should add the research great dynamics on the one hand at this, promote the fermentation transformation technology in the utilization of the field of Chinese medicines, can better utilize Chinese medicine.
Hong Kong traditional Chinese medical science doctor Wu Zhiyong with teach Lin Lushan interiorly and cooperate, take the lead in using the biotechnology fermented tcm to succeed, it has thoroughly changed the conventional machining process of frying in shallow oil, boil, endure, refine, steam, soaking, and drug effect is improved, the contained astragalus polysaccharides of fermentation astragalus mostly is 5 times of traditional handicraft most, and 1/28 of the medicine that the consumption of the zoopery proof fermentation Radix Astragali is only made for traditional method can produce identical drug effect.The people such as Dai Wansheng use fermentation method to concoct Radix Et Rhizoma Rhei, have changed the content of the anthraquinone component of Radix Et Rhizoma Rhei; Anthraquinone component is one of effective ingredient of Radix Et Rhizoma Rhei, after culture propagation, its total anthraquinones content only slightly reduces, preserved more total Radix Et Rhizoma Rhei anthraquinone than traditional method, and play reducing in conjunction with the class anthraquinone content of discharge function, in clinical practice, relaxed the high strong discharge function of Radix Et Rhizoma Rhei and reached the gastrointestinal untoward reaction; And 6 times of left and right have been increased as the content of the sequestered anthraquinone of antibiotic, the main effective ingredient of antineoplastic.Nearly 60 kinds of Chinese medicines such as Herba Ephedrae, Lay Fructus Solani melongenae, Flos Lonicerae, Fructus Forsythiae, Ganoderma, Radix Notoginseng, Rhizoma Belamcandae, Spica piperis betlis, Semen Armeniacae Amarum have been carried out fermentation method research at present.But the research that applies to aspect the Radix Paeoniae Alba at fermentation method has no report.
Because the contained chemical composition of the Radix Paeoniae Alba is subject to the impact of external condition in the Preparation process process, fermentation method can be preserved its original chemical composition preferably.In Radix Paeoniae Alba Study on Fermentation, strain used, pharmaceutical decocting piece processing method, fermentation time and fermentation temperature etc. are carried out integrated survey, to draw the optimal processing parameter of Radix Paeoniae Alba fermentation research.Thereby further Radix Paeoniae Alba industrialization processing technique and the specifications of quality are carried out further investigated, so that Radix Paeoniae Alba processing specification, prepared slice quality standardization, controlledization.
This outlet decoction pieces product of the Radix Paeoniae Alba that this project alternatives enterprise has self-character is object of study, relevant requirements according to the outlet About The Quality of Sliced Herbal Medicine, adopt orthogonal test and parallel test etc., in conjunction with the existing production technology of enterprise and experience, carry out Radix Paeoniae Alba decoction pieces fermentation processing technique standardization and quality standard research, make the fermentation Radix Paeoniae Alba form the industrialized manufacturing technique of standard; Respectively take peoniflorin and Radix Paeoniae Alba total glycosides-water-soluble extractives etc. as the quality index, set up its quality standard, and reach stable and controllable for quality simultaneously, for outlet Radix Paeoniae Alba industrialization processing provides foundation.
By research, the standardization processing procedure of fermentation Radix Paeoniae Alba decoction pieces is set up in research first, makes its steady quality, controlled; The peoniflorin of fermentation Radix Paeoniae Alba decoction pieces and the enterprise-quality standard of Radix Paeoniae Alba total glycosides-water-soluble extractives have been set up in research first.This project achievement in research has been implemented industrialization production and application at present, greatly improved labor productivity, effectively reduced processing cost, the fermentation Radix Paeoniae Alba is stable and controllable for quality, improve fermentation Radix Paeoniae Alba competitiveness in the international market, and produced good economic benefit and social benefit.
One, fermentation quality control of Paeonia lactiflora normalization
1. experiment material
1.1. medical material: white Peony Root is bought in medical material market, Haozhou, and the thousand medical herbs industry decoction pieces factories through Haozhou city, Anhui Province are accredited as the dry root of ranunculaceae plant Radix Paeoniae Paeonia lactiflora Pall., and the fermentation Radix Paeoniae Alba is the Radix Paeoniae Alba decoction pieces product that our company produces.
1.2 instrument reagent: Agilent1200series (U.S. Agilent company); Sartorius series 100,000/electronic analytical balance (Switzerland); Excellent general ultrapure water machine; RE-52AA rotary evaporator (Shanghai Yarong Biochemical Instrument Plant); The multiplex vacuum pump of SHB-III circulating water type (Zhengzhou Greatwall Scientific Industrial ﹠ Trading Co., Ltd.); Chinese medicine material crushing machine (in Fuyang, ancient cooking vessel machinery company limited is sold); Vacuum drying oven (the accurate experimental facilities company limited in Shanghai); The peoniflorin reference substance is purchased from Nat'l Pharmaceutical ﹠ Biological Products Control Institute; Acetonitrile, methanol are chromatographically pure, and water is ultra-pure water, and all the other reagent are analytical pure.
2. the foundation of paeoniflorin content assay method
2.1 the preparation of solution
2.1.1 the preparation of reference substance solution
It is appropriate that precision takes the peoniflorin reference substance, adds methanol and make the solution that every 1ml contains 60 μ g, and get final product.
2.1.2 the preparation of need testing solution
Get the about 0.1g of powder in the fermentation Radix Paeoniae Alba, accurately weighed, put in the 50ml measuring bottle, add Diluted Alcohol 35ml, supersound process (power 240W, frequency 45kHz) 30 minutes is taken out, and lets cool, and adds methanol to scale, shakes up, and filters, and gets subsequent filtrate, and get final product.
2.2 chromatographic condition
Take octadecylsilane chemically bonded silica as filler; Take acetonitrile-0.1% phosphoric acid solution (14: 86) as mobile phase; The detection wavelength is 230nm.
2.3 methodological study
2.3.1 linear relationship is investigated
Accurate peoniflorin reference substance solution 5 μ l, 8 μ l, 10 μ l, 12 μ l, the 15 μ l of drawing, the injection liquid chromatography by 2.2 lower chromatographic condition analyses, records chromatogram.Take the sample introduction quality as abscissa, the peoniflorin peak area is vertical coordinate drawing standard curve, gets the regression equation of peoniflorin:
Y=1160x-27.09, r=0.9999 shows that peoniflorin is good in 0.30～0.90 μ g scope internal linear relation.The results are shown in Table 1-1 and Fig. 1.
The linear relationship of table 1-1 peoniflorin is investigated result
2.3.2 system suitability
Inject respectively reference substance solution and the sample liquid of peoniflorin according to 2.2 lower chromatographic conditions and measure, result shows, under this chromatographic condition, calculates according to the chromatographic peak of peoniflorin, number of theoretical plate is higher, separating degree and tailing factor all up to specification.Consider the difference of the conditions such as the performance of different chromatographic columns and mobile phase ratio, number of theoretical plate calculates by the peoniflorin chromatographic peak should be not less than 2000.Chromatogram is seen Fig. 2, Fig. 3.
2.3.3 precision test:
The accurate peoniflorin reference substance solution 10 μ l that draw under above-mentioned chromatographic condition, repeat sample introduction 6 times, record chromatogram, calculate the RSD of retention time and peak area, the results are shown in Table 1-2.
Table 1-2 Precision test result (n=6)
RSD all less than 3%, shows that instrument precision is good as a result, meets the requirement of quantitative analysis.
2.3.4 stability test
Take in the fermentation Radix Paeoniae Alba of same batch of methodology test, by preparing need testing solution under the 2.1.2 item, respectively at 0h, 3h, 6h, 9h, 12h, 15h, the accurate need testing solution 10 μ l that draw, the injection liquid chromatography detects by above-mentioned chromatographic condition, records the peak area of peoniflorin, calculates RSD.The results are shown in Table 1-3.
Table 1-3 stability test result (n=6)
RSD all less than 3%, shows that need testing solution is better at 15 hours internal stabilities as a result.
2.3.5 replica test
Get with a collection of test sample 6 increments, by preparing need testing solution under the 2.1.2 item, get 10 μ l injection liquid chromatographies, detect by above-mentioned chromatographic condition, record peak area and retention time, calculate content and the RSD% of peoniflorin in the fermentation Radix Paeoniae Alba.The results are shown in Table 1-4.
Table 1-4 replica test result (n=5)
RSD all less than 3%, shows the repeatability of test better as a result.
2.3.6 application of sample recovery test
Precision takes totally 9 parts of the medicinal powder 0.05g of appropriate known paeoniflorin content, and is accurately weighed.Add each three parts of peoniflorin reference substance (concentration is 1mg/mL) 1mL, 2mL, 3mL, by preparing need testing solution under the 1.2.2 item.Get subsequent filtrate, upper HPLC chromatograph detects.By formula [response rate=(amount of recording-original amount)/addition] calculates average recovery, gets average recovery rate and RSD%, the results are shown in Table 1-5.Result shows that the average recovery of this method meets the requirements in the amount that adds peoniflorin.Measurement result sees Table 1-5.
Table 1-5 application of sample recovery test result (n=6)
Table 1-5 result shows: average recovery test average recovery rate value is that 99.78%, RSD% is 2.36%, less than 3%, meets the average recovery test determination.
3. the foundation of benzoic acid content assay method
3.1 the preparation of solution
3.1.1 the preparation of reference substance solution
It is appropriate that precision takes the benzoic acid reference substance, adds the solution that methanol is made 0.005mg/ml, and get final product.
3.1.2 the preparation of need testing solution
Accurately weighed Radix Paeoniae Alba coarse powder 1.0g puts in tool plug conical flask, and precision adds methanol 25mL, weighed weight, and soaked overnight, supersound process 1h lets cool, more weighed weight, supplies less loss weight with methanol, shakes up, and filters, and collects subsequent filtrate, and get final product.
3.2 chromatographic condition
Take octadecylsilane chemically bonded silica as filler; Take mobile phase: methanol-0.1% phosphoric acid solution (40: 60) is as mobile phase; The detection wavelength is 232nm; Column temperature is 30 ℃.
3.3 methodological study
3.3.1 linear relationship is investigated
Accurate peoniflorin reference substance solution 4 μ l, 6 μ l, 8 μ l, 10 μ l, 12 μ l, the 14 μ l of drawing, the injection liquid chromatography by 3.2 lower chromatographic condition analyses, records chromatogram.Take sample size as abscissa, the benzoic acid peak area is vertical coordinate drawing standard curve, gets benzoic regression equation:
Y=7764x-3.386, r=0.9999 shows that benzoic acid is good in 0.01～0.06 μ g scope internal linear relation.The results are shown in Table 1-6 and Fig. 4.
The benzoic linear relationship of table 1-6 is investigated result
3.3.2 system suitability
Inject respectively benzoic reference substance solution and sample liquid is measured according to 3.2 lower chromatographic conditions, result shows, under this chromatographic condition, calculates according to benzoic chromatographic peak, number of theoretical plate is higher, separating degree and tailing factor all up to specification.Consider the difference of the conditions such as the performance of different chromatographic columns and mobile phase ratio, number of theoretical plate calculates by the benzoic acid chromatographic peak should be not less than 2000.
3.3.3 precision test:
The accurate benzoic acid reference substance solution 10 μ l that draw under above-mentioned chromatographic condition, repeat sample introduction 6 times, record chromatogram, calculate the RSD of retention time and peak area, the results are shown in Table 1-7.
Table 1-7 Precision test result (n=6)
RSD all less than 3%, shows that instrument precision is good as a result, meets the requirement of quantitative analysis.
3.3.4 stability test
Take in the fermentation Radix Paeoniae Alba of same batch of methodology test, by preparing need testing solution under the 3.1.2 item, respectively at 0h, 3h, 6h, 9h, 12h, 15h, the accurate need testing solution 10 μ l that draw, the injection liquid chromatography detects by above-mentioned chromatographic condition, records benzoic peak area, calculates RSD%.The results are shown in Table 1-8.
Table 1-8 stability test result (n=6)
RSD all less than 3%, shows that need testing solution is better at 15 hours internal stabilities as a result.
3.3.5 replica test
Get with a collection of test sample 6 increments, by preparing need testing solution under the 3.1.2 item, get 10 μ l injection liquid chromatographies, detect by above-mentioned chromatographic condition, record peak area and retention time, calculate benzoic content and RSD% in the fermentation Radix Paeoniae Alba.The results are shown in Table 1-9.
Table 1-9 replica test result (n=5)
RSD all less than 3%, shows the repeatability of test better as a result.
3.3.6 application of sample recovery test
Precision takes totally 9 parts of the medicinal powder 0.5g of appropriate known paeoniflorin content, and is accurately weighed.Add each three parts of benzoic acid reference substance (concentration is 0.005mg/mL) 2mL, 3mL, 4mL, by preparing need testing solution under the 1.2.2 item.Get subsequent filtrate, upper HPLC chromatograph detects.By formula [response rate=(amount of recording-original amount)/addition] calculates average recovery, gets average recovery rate and RSD%, the results are shown in Table 1-10.Result shows that the average recovery of this method is to add benzoic amount to meet the requirements.
Table 1-10 application of sample recovery test result (n=6)
Table 1-10 result shows: average recovery test average recovery rate value is that 98.84%, RSD% is 0.96%, less than 3%, meets the average recovery test determination.
4. the research of water-soluble extractives inspection method
With reference to " under Chinese pharmacopoeia 2010 version (an one) " Extract mensuration " (appendix XA) " water-soluble extractives algoscopy (hot dipping) ".The sample thief powder is put 60 ℃ of dry 1h in baking oven, cools, and gets 2g, accurately weighed, to put in the 250mL conical flask, precision adds distilled water 100mL, jam-pack, weighed weight, standing 1h, condensing reflux keeps little 1h that boils, let cool rear taking-up conical flask, weigh, benefit is heavy, shakes up, dry filter filters, and precision measures 25mL and puts in the dry evaporating dish of constant weight, water bath method, 105 ℃ of dry 3h put exsiccator cooling, fast precision weighing.Measure its leaching rate, leaching rate (%)=(M ware+extractum-M ware) * 4/ sampling amount * 100.
Table 1-alcoholic solution determination of extractives result
Two, fermentation Radix Paeoniae Alba decoction pieces Research on processing technology
The concocting method of the Radix Paeoniae Alba and the quality of processed product can directly exert an influence to clinical efficacy, make a general survey of the successive dynasties book on Chinese herbal medicine, and the concocting method of the Radix Paeoniae Alba reaches more than several.The Radix Paeoniae Alba is wanted one of export varieties for the corporate boss, in the course of processing, owing to not setting up objective data metallization processes parameter, the boiling time often occurs, add the yellow wine amount, aspect many dependence experience such as parch duration and degree of heating, parch time judges the processed product quality, lack objective index control foundation, cause Radix Paeoniae Alba quality uneven; In addition, due to the enterprise-quality standard of not setting up Radix Paeoniae Alba decoction pieces, to a certain extent, affected the outlet of enterprise's Radix Paeoniae Alba decoction pieces; Be further lifting, enlarge quality and quantity that the prepared slices of Chinese crude drugs export, reply foreign technology barrier promotes its international competitiveness, expands the prepared slices of Chinese crude drugs and exports the international market.
Because the contained chemical composition of the Radix Paeoniae Alba is subject to the impact of external condition in the Preparation process process, fermentation method carries out at normal temperatures, can preserve preferably its original chemical composition.For this reason, this problem pin fermentation Radix Paeoniae Alba processing technique that thousand medical herbs industry decoction pieces companies have a self-character take the Bozhou is the basis, carry out systematic study for the major influence factors in the fermentation Radix Paeoniae Alba course of processing, formulate the standardization flow process of processing technique, and set up the quality standard of the fermentation Radix Paeoniae Alba, the international market of further expanding the fermentation Radix Paeoniae Alba for enterprise provides foundation.
1. the work flow of fermentation Radix Paeoniae Alba decoction pieces
BAIYAO medical material → boil medical material → dry shaping to the saturating heart → remove the peel → dry → section → Radix Paeoniae Alba decoction pieces → pulverize → clean → Radix Paeoniae Alba granule → add strain to ferment → ferment → be dried to fermentation Radix Paeoniae Alba.
2. in the fermentation Radix Paeoniae Alba course of processing, sample size is measured
Carry out paeoniflorin content by " one, 2 " lower need testing solution preparation and condition determination and measure, record chromatogram, the content of peoniflorin in calculation sample the results are shown in Table 2-1.
Table 2-1 sample size is measured (n=2)
Experimental result shows, peoniflorin is easily destroyed in the Radix Paeoniae course of processing, and the average content of peoniflorin in the different processed products of Radix Paeoniae is followed successively by: the fermentation Radix Paeoniae Alba (3.39%)＞Radix Paeoniae Alba (2.89%)＞Radix Paeoniae Alba (2.77%)＞Radix Paeoniae Alba (parched) (2.54%); The average content of benzoic acid in the different processed products of Radix Paeoniae is followed successively by: the fermentation Radix Paeoniae Alba (3.39%)＜Radix Paeoniae Alba (2.89%)＜Radix Paeoniae Alba (parched) (2.54%)＜Radix Paeoniae Alba (2.77%), the fermentation Radix Paeoniae Alba processing technique that illustrative experiment is groped can guarantee the quality at the decoction pieces of the course of processing.
Three, pilot plant test
On the basis of above-mentioned experimental studies results, experience and existing appointed condition in conjunction with the thousand medical herbs industry processing and fermentation Radix Paeoniae Albas amplify.
1. Radix Paeoniae Alba work flow ferments
Carry out middle trial production according to fermentation Radix Paeoniae Alba best processing technology, Radix Paeoniae Alba pilot scale finished product must ferment.
2. fermentation Radix Paeoniae Alba pilot scale sample quality is analyzed
2.1 sample solution preparation and paeoniflorin content are measured
Undertaken by " under a 2.1.2 item " and " under one 2.2 " method.
2.2 measurement result: see Table 2-2
Table 2-2 fermentation Radix Paeoniae Alba pilot plant test result
Quality results the analysis showed that, fermentation Radix Paeoniae Alba pilot experiment feasible process, and quality meets version " Chinese pharmacopoeia relevant requirements in 2010.
3. brief summary and analysis
This this outlet decoction pieces product with self-character of the Radix Paeoniae Alba of studying to ferment is object of study, relevant requirements according to the outlet About The Quality of Sliced Herbal Medicine, in conjunction with the existing production technology of enterprise and experience, carry out the fermentation Radix Paeoniae Alba this novel decoction pieces processing technique standardization and quality standard research, by investigation and the systematic study to many factors (adding strain kind and quantity, fermentation time, fermentation temperature etc.) in fermentation Radix Paeoniae Alba decoction pieces secondary industry production technology, fermentation Radix Paeoniae Alba industrialized manufacturing technique and technology objective parameter have been set up.Thereby make the fermentation Radix Paeoniae Alba form the industrialized manufacturing technique of standard, and reach stable and controllable for quality, for fermentation Radix Paeoniae Alba industrialization processing provides foundation.
Aspect the quality control of the fermentation Radix Paeoniae Alba, when investigating index, paeoniflorin content, the water-soluble extractives content measuring standard of the fermentation Radix Paeoniae Alba have been formulated first take fermentation Radix Paeoniae Alba decoction pieces outward appearance, color and luster;
In addition, fermentation Radix Paeoniae Alba quality control aspect, though though peoniflorin has analgesia, calmness, anticonvulsant action, to immune effect, to the effect of smooth muscle, the activity of the aspects such as antiinflammatory action, for one of active component of the Radix Paeoniae Alba, be derivant but peoniflorin is pinane, unstable, can be become by wherein contained dilute acid hydrolysis glucose and volatile pinane derivative under certain temperature and humidity, and drug effect is reduced.Fermentation method can keep peoniflorin preferably in the Preparation process process, provide reference frame for the quality control of the fermentation Radix Paeoniae Alba when having guaranteed drug effect.
With reference to 2010 editions " Chinese pharmacopoeia high effective liquid chromatography for measuring, measurement results: contain paeoniflorin Paeo-niflorin in every gram processed product and be not less than 3.3%.
The experiment of advantage
The Radix Paeoniae Alba decoction pieces of tradition Preparation process and the Radix Paeoniae Alba decoction pieces of this Preparation process are by " comparing research under 2010 editions detections of Chinese pharmacopoeia.Result of the test shows, after adopting concocting method of the present invention to concoct, reduces the loss of peoniflorin, and the content that makes peoniflorin increases without significant change and the finished product content concocted than traditional method.The mouthfeel of the more traditional Radix Paeoniae Alba processed product of the finished product of concocting out changes obviously, and bitterness is removed substantially, has reduced the property of the bitter cold of crude drug.And can make its cell wall breaking, thereby macromole changes micromolecule into, more be conducive to absorb.
Claims (4)
1. a Radix Paeoniae Alba processed product, is characterized in that, contains paeoniflorin in every gram processed product and be not less than 1.6%.
2. Radix Paeoniae Alba processed product according to claim 1, is characterized in that, contains paeoniflorin in every gram processed product and be not less than 1.8%.
3. a method for preparing the described Radix Paeoniae Alba processed product of claim 1 or 2, is characterized in that, it comprises the steps:
A, the rhizome of getting the fresh Radix Paeoniae Alba are cleaned, section, make Radix Paeoniae Alba sheet;
B, the Radix Paeoniae Alba sheet that a step is chosen soak, mix with Testa Tritici distillation, drying, redistillation, drying;
C, yeast and distilled water are cultivated, then carried out ferment at constant temperature with the Radix Paeoniae Alba that the b step makes, the Radix Paeoniae Alba removal of impurity after fermentation, drying, and get final product.
4. the method for Radix Paeoniae Alba processed product according to claim 3, it is characterized in that: the described ferment at constant temperature temperature of c step is 25 ℃-28 ℃, and time: 12h-24h, pH value are 5-5.5.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104771460A (en) * | 2015-04-20 | 2015-07-15 | 安徽精诚本草中药饮片有限公司 | Fabrication method of radix paeoniae alba decoction pieces |
| CN105748622A (en) * | 2016-04-30 | 2016-07-13 | 三株福尔制药有限公司 | Probiotic fermented traditional Chinese medicine composition for spasmolysis and analgesia and preparation method and application thereof |
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- 2013-01-28 CN CN2013100304534A patent/CN103099856A/en active Pending
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| 董广潮: "浅析中药白芍炮制及其历史沿革", 《中国现代药物应用》 * |
| 马玉等: "白芍原药材与不同炮制加工品的高效液", 《成都中医药大学学报》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104771460A (en) * | 2015-04-20 | 2015-07-15 | 安徽精诚本草中药饮片有限公司 | Fabrication method of radix paeoniae alba decoction pieces |
| CN105748622A (en) * | 2016-04-30 | 2016-07-13 | 三株福尔制药有限公司 | Probiotic fermented traditional Chinese medicine composition for spasmolysis and analgesia and preparation method and application thereof |
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Application publication date: 20130515 |