CN117322424A - Pesticide containing oxazine amide and cyantraniliprole and application thereof - Google Patents
Pesticide containing oxazine amide and cyantraniliprole and application thereof Download PDFInfo
- Publication number
- CN117322424A CN117322424A CN202310654739.3A CN202310654739A CN117322424A CN 117322424 A CN117322424 A CN 117322424A CN 202310654739 A CN202310654739 A CN 202310654739A CN 117322424 A CN117322424 A CN 117322424A
- Authority
- CN
- China
- Prior art keywords
- cyantraniliprole
- pesticide
- oxazine
- amide
- test
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/48—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with two nitrogen atoms as the only ring hetero atoms
- A01N43/58—1,2-Diazines; Hydrogenated 1,2-diazines
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/48—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with two nitrogen atoms as the only ring hetero atoms
- A01N43/56—1,2-Diazoles; Hydrogenated 1,2-diazoles
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P7/00—Arthropodicides
- A01P7/04—Insecticides
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Abstract
The invention discloses a pesticide containing oxaziclomefone and cyantraniliprole and application thereof, wherein the pesticide comprises an active component which consists of oxazine amide and cyantraniliprole; the mass ratio of the oxazine amide to the cyantraniliprole is 1:30-30:1. The oxazine and the cyantraniliprole are specifically selected and compounded according to the proportion provided by the invention, so that the oxazine and the cyantraniliprole can generate a synergistic effect, and the formed pesticide has a good insecticidal effect and a large control spectrum, and particularly has a good control effect on spodoptera frugiperda, cowpea thrips and liriomyza sativae.
Description
Technical Field
The invention relates to the technical field of pesticides, in particular to a pesticide containing oxaziclomefone and cyantraniliprole and application thereof.
Background
Spodoptera frugiperda (Spodoptera frugiperda) belongs to the family of lepidoptera, and is a omnivorous agricultural pest. Spodoptera frugiperda has the characteristics of strong flying ability, large propagation quantity, heavy overeating, wide host range and the like, and is mainly eaten by gramineous plants, and main crops such as corn, rice, sorghum, cotton and the like can be used as the larva stage of the spodoptera frugiperda, and various vegetables, fruits and ornamental plants.
The cowpea thrips are the collective name of common thrips (Megalurothrips usitatus Bagrall) and frankliniella occidentalis (Franeliniella intonsa Trybom), belong to the family of thrips of the order thysanoptera of the class insect, are agriculturally important economic pests, can harm various crops such as cowpea, soybean, peanut and the like, can cause cowpea thrips adults and nymphs to suck juice of tender tissues and organs of cowpea plants by using a file suction type mouth gag, are harmful stems, leaves, flowers and pods, cause leaf shrinkage deformity, plant wilting, flower falling pod deformity, young pod deformity and the like, seriously affect the yield and quality of cowpea, and are one of the most serious pests in cowpea production.
Liriomyza sativae (Liriomyza sativae Blanchard) belongs to the family diptera and is a multi-feeding pest that harms vegetables, flowers and other plants. The liriomyza sativae hosts are wide in range and spread rapidly, and cause great threat to the production of vegetables in China, 17-20 generations can occur in Guangzhou throughout the year, 9-11 generations can occur in Henan Luoyang, the generation overlap phenomenon exists, vegetables such as cowpea are liriomyza sativae susceptible plants, the occurrence is heaviest, 7-8 months are peak periods, the insect plant rate can reach 100%, the insect leaf rate is up to more than 80%, serious yield reduction and even sterilization are caused, and the damage loss of the liriomyza sativae to cowpea is serious.
The oxazine amide is a pyrazole amide compound, and the English common name is as follows: dimropyridaz; trade name: axalon TM The method comprises the steps of carrying out a first treatment on the surface of the IUPAC name: 1- [ (1 RS) -1, 2-dimethylpropyl]-N-ethyl-5-methyl-N-pyridazin-4-yl-1H-pyrazole-4-carboxamide; CAS loginNumber: 1403615-77-9; the molecular formula: c (C) 16 H 23 N 5 O; the product of the oxazine amide has excellent performance and better systemic conductivity. The insecticidal composition is mainly used for controlling pests such as lepidoptera (such as chilo suppressalis, asparagus caterpillar, plutella xylostella, spodoptera frugiperda and the like), coleoptera (such as beetles, corn rootworm, potato leaf beetles, yellow leaf beetles and the like), diptera (such as flies, mosquitoes, vegetable leaf miner and the like), hemiptera (such as aphids, plant hoppers, psyllids, whiteflies and the like), thysanoptera (such as thrips, frankliniella, palmi thrips, thrips and the like), isoptera (such as termites and the like), cockroaches, ants and the like, and is particularly effective on sucking mouthparts pests such as aphids, whiteflies, psyllids and the like; the structural formula is as follows:
cyantraniliprole (cyantraniliprole), chemical name: 3-bromo-1- (3-chloro-2-pyridinyl) -N- [ 4-cyano-2-methyl-6- [ (methylamino) hydroxy]Phenyl group]-1H-pyrazole-5-carboxamide of the chemical formula: c (C) 19 H 14 BrClN 6 O 2 . The cyantraniliprole is formed by changing various polar groups on benzene rings, has higher efficiency and wider application range, can effectively prevent and treat lepidoptera, hemiptera and coleopteran pests, and has the structural formula shown as follows:
because of the wide planting area of corn and vegetables, and the rapid propagation speed of spodoptera frugiperda, cowpea thrips and liriomyza sativae, the prevention and control difficulty is high and the hazard is heavy. Many of the existing common control agents are old agents with longer time and higher resistance, and the control effect is poor. Therefore, the prior art has the advantages that components with different action mechanisms are compounded, and then insect pest control is carried out, so that the occurrence of insect pest drug resistance is avoided. However, after compounding, whether the compounding is synergistic, additive or antagonistic is judged according to the actual application effect, but the compounding formula with antagonistic is obtained under normal conditions, the compounding formula with additive is few, and the compounding with obvious synergistic effect and high co-toxicity coefficient is less. Therefore, providing a compound formulation with excellent prevention effect is a problem to be solved in the prior art.
Disclosure of Invention
In view of the above problems, the present invention provides a pesticide containing oxaziclomefone and cyantraniliprole and an application thereof, which can solve the problem that spodoptera frugiperda, cowpea thrips and liriomyza in the prior art are difficult to control.
Specifically, the invention provides a pesticide containing oxaziclomefone and cyantraniliprole, which comprises an active component, wherein the active component consists of oxazine and cyantraniliprole; the mass ratio of the oxazine amide to the cyantraniliprole is 1:30-30:1.
In an alternative embodiment, the mass ratio of the oxazamate to the cyantraniliprole is 1:7 to 7:1.
In an alternative embodiment, the mass ratio of the oxazamate to the cyantraniliprole is 1:1.
In an alternative embodiment, the ratio of the mass of the oxazamate to the mass of the pesticide insecticide is 1-30%.
In an alternative embodiment, the ratio of the mass of the oxazamate to the mass of the pesticide insecticide is 10%.
In an alternative embodiment, the ratio of the mass of the cyantraniliprole to the mass of the pesticide is 1-30%.
In an alternative embodiment, the ratio of the mass of the cyantraniliprole to the mass of the pesticide is 10%.
In an alternative embodiment, the pesticide formulation is a liquid formulation.
In an alternative embodiment, the pesticide further comprises an adjuvant comprising a surfactant, an anti-freeze agent, a solvent, and deionized water.
The surfactant includes, but is not limited to, one or more of nonylphenol polyoxyethylene ether, nonylphenol polyoxyethylene ether phosphate, alkylphenol polyoxyethylene ether phosphate, alkylphenol polyoxyethylene ether polyoxypropylene ether, alkylphenol formaldehyde resin polyoxyethylene ether, phenethyl phenol polyoxypropylene polyoxyethylene ether, phenethyl phenol polyoxyethylene ether phosphate, benzyl phenol polyoxyethylene ether, phenethyl phenol polyoxyethylene ether, fatty alcohol polyoxyethylene ether, laurinol polyoxyethylene ether, fatty amine polyoxyethylene ether, castor oil ethylene oxide adduct, sorbitan fatty acid ester ethylene oxide adduct, calcium dodecylbenzenesulfonate, sulfated castor oil, fatty alcohol sulfate, fatty alcohol polyoxyethylene ether sulfate, fatty acid polyoxyethylene ester phosphate, alkylamine polyoxyethylene ether phosphate, alkylphenol polyoxyethylene ether formaldehyde condensate, polyvinyl alcohol monooleate, polyethylene glycol monooleate, polyoxyethylene polyoxypropylene block copolymer, polycarboxylate, polyacrylic acid, sodium alkyl naphthalene sulfonate fatty alcohol polyoxyethylene ether, sorbitan monooleate, polyoxyethylene sorbitan monooleate, castor oil polyoxyethylene ether;
the antifreezing agent comprises one or more of ethylene glycol, glycerol, propylene glycol, urea, methanol, ethanol, isopropanol, diethylene glycol, ethylene glycol butyl ether, propylene glycol butyl ether, ethylene glycol butyl ether acetate and isooctanol;
the solvent includes, but is not limited to, one or more of mineral spirits, toluene, xylene, methanol, ethanol, methyl oleate, cyclohexanone, vegetable oils, methyl vegetable oils, dimethylformamide, acetonitrile, polyethylene glycol, sunflower amide, tributyl phosphate, butyrolactone, N-long chain alkyl pyrrolidone, octyl pyrrolidone, isooctyl alcohol.
The invention provides application of the pesticide according to any one of the above to prevention and treatment of spodoptera frugiperda, cowpea thrips and liriomyza sativae.
The invention has the beneficial effects that:
in the pesticide containing the oxazine amide and the cyantraniliprole, the oxazine amide and the cyantraniliprole are specifically selected and compounded according to the proportion provided by the invention, so that the oxazine amide and the cyantraniliprole can produce a synergistic effect, the formed pesticide has good insecticidal effect and a large control spectrum, and particularly has good control effect on spodoptera frugiperda, cowpea thrips and liriomyza sativae.
Further, when the mass ratio of the oxazine amide to the cyantraniliprole is 1:7-7:1, the compound medicament has a synergistic effect on cowpea thrips. Especially when the mass ratio of the oxazine amide to the cyantraniliprole is 1:1, the CTC value is 167.32, and the synergistic effect is remarkable.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention more clear, the technical solutions of the embodiments of the present invention will be clearly and completely described below. The specific conditions are not noted in the examples and are carried out according to conventional conditions or conditions recommended by the manufacturer.
The invention provides a pesticide containing oxaziclomefone and cyantraniliprole, which comprises an active component, wherein the active component consists of oxazine amide and cyantraniliprole; the mass ratio of the oxazine amide to the cyantraniliprole is 1:30-30:1. According to the embodiment of the invention, the oxazine and the cyantraniliprole are specifically selected and compounded according to the proportion of 1:30 to 30:1, so that the oxazine and the cyantraniliprole can generate a synergistic effect, and the formed pesticide has a good insecticidal effect and a large prevention and control spectrum, and particularly has a good prevention and control effect on spodoptera frugiperda, cowpea thrips and liriomyza sativae.
The pesticide provided by the invention is used for preventing and controlling insect pests of crops including, but not limited to, spodoptera frugiperda, thrips cowpea, liriomyza sativae and the like, and only has better preventing and controlling effect on spodoptera frugiperda, thrips cowpea and liriomyza sativae.
Specifically, the mass ratio of the oxazine amide to the cyantraniliprole is 1:30, 1:10, 1:3, 3:7, 3:5, 1:1, 5:3, 7:3, 3:1, 15:1, 30:1. Preferably, the mass ratio of the chlorfluazuron and the oxaziclomefone is 3:5, 5:3 or 1:1.
Further, the pesticide further comprises an auxiliary agent, wherein the auxiliary agent comprises a surfactant, an antifreezing agent, a solvent and deionized water. The surfactant includes, but is not limited to, one or more of nonylphenol polyoxyethylene ether, nonylphenol polyoxyethylene ether phosphate, alkylphenol polyoxyethylene ether phosphate, alkylphenol polyoxyethylene ether polyoxypropylene ether, alkylphenol formaldehyde resin polyoxyethylene ether, phenethyl phenol polyoxypropylene polyoxyethylene ether, phenethyl phenol polyoxyethylene ether phosphate, benzyl phenol polyoxyethylene ether, phenethyl phenol polyoxyethylene ether, fatty alcohol polyoxyethylene ether, laurinol polyoxyethylene ether, fatty amine polyoxyethylene ether, castor oil ethylene oxide adduct, sorbitan fatty acid ester ethylene oxide adduct, calcium dodecylbenzenesulfonate, sulfated castor oil, fatty alcohol sulfate, fatty alcohol polyoxyethylene ether sulfate, fatty acid polyoxyethylene ester phosphate, alkylamine polyoxyethylene ether phosphate, alkylphenol polyoxyethylene ether formaldehyde condensate, polyvinyl alcohol monooleate, polyethylene glycol monooleate, polyoxyethylene polyoxypropylene block copolymer, polycarboxylate, polyacrylic acid, sodium alkyl naphthalene sulfonate fatty alcohol polyoxyethylene ether, sorbitan monooleate, polyoxyethylene sorbitan monooleate, castor oil polyoxyethylene ether;
the antifreezing agent comprises one or more of ethylene glycol, glycerol, propylene glycol, urea, methanol, ethanol, isopropanol, diethylene glycol, ethylene glycol butyl ether, propylene glycol butyl ether, ethylene glycol butyl ether acetate and isooctanol;
the solvent includes, but is not limited to, one or more of mineral spirits, toluene, xylene, methanol, ethanol, methyl oleate, cyclohexanone, vegetable oils, methyl vegetable oils, dimethylformamide, acetonitrile, polyethylene glycol, sunflower amide, tributyl phosphate, butyrolactone, N-long chain alkyl pyrrolidone, octyl pyrrolidone, isooctyl alcohol.
The features and capabilities of the present invention are described in further detail below in connection with particular embodiments.
Example 1:20% cyantraniliprole-oxazine amide microemulsion (1:1)
10.0g of cyantraniliprole, 10.0g of oxazine, 10.0g of sorbitan monooleate, 3.0g of polyoxyethylene sorbitan monooleate, 1.5g of castor oil polyoxyethylene ether, 2.0g of ethylene glycol and 7.5g of sunflower amide are mixed and stirred into a uniform and transparent oil phase, and then 56.0g of deionized water is slowly added while stirring to form uniform and transparent liquid, so that the pesticide is obtained.
It should be noted that the examples of the present invention only exemplify microemulsions, but it is understood that other types of liquid formulations are also within the scope of the examples of the present invention.
Comparative example 1:10% cyantraniliprole microemulsion
10.0g of cyantraniliprole, 6.0g of sorbitan monooleate, 2.0g of polyoxyethylene sorbitan monooleate, 1.2g of castor oil polyoxyethylene ether, 2.0g of ethylene glycol and 3.5g of sunflower amide are mixed and stirred into a uniform and transparent oil phase, and then 75.3g of deionized water is slowly added while stirring to form uniform and transparent liquid, so that the pesticide is obtained.
Comparative example 2:10% oxazin-chlorfenapyr microemulsion
10.0g of oxazine amide, 5.0g of sorbitan monooleate, 3.0g of polyoxyethylene sorbitan monooleate, 1.0g of castor oil polyoxyethylene ether, 2.5g of ethylene glycol and 4.0g of sunflower amide are mixed and stirred into a uniform and transparent oil phase, and then 74.5g of deionized water is slowly added while stirring to form uniform and transparent liquid, so that the pesticide is obtained.
The pesticidal agents provided in the above examples and comparative examples were used in the following test examples to verify the toxicity and efficacy of pesticidal agents.
Test example 1 biological Activity test on Spodoptera frugiperda
Pesticide part 10 with reference to NYT 1154.10-2008 pesticide indoor bioassay test guidelines: the test is carried out by an artificial feed mixing method, and the optimal mixing mass ratio of the two pesticides is determined by measuring the effects of the mixture of the oxazamate, the cyantraniliprole and different mass ratios of the oxazamate and the cyantraniliprole on spodoptera frugiperda.
A. Spodoptera frugiperda (Spodoptera frugiperda) was harvested from shandong in the near-to-Yi, and then continuously fed indoors at a temperature of 25 ℃ ± 1 ℃ and a humidity of 60% -70% with photoperiod L: d=16:8 without any insecticide contact.
B. The test agent is 90% oxazine amide and 94% cyantraniliprole original drug
C. The mixing proportion of the oxazine amide and the cyantraniliprole is as follows: the 3:1, 7:3, 5:3, 1:1, 3:5, 3:7, 1:3 formulation concentrations are shown in Table 1:
TABLE 1 dosage ratio of oxazine and cyantraniliprole
D. Toxicity test method
Feed mixing method reference NYT 1154.10-2008 pesticide indoor bioassay test guidelines pesticide part 10: a manual feed mixing method; firstly diluting mother liquor with acetone to 6 series gradients, then respectively dripping 1mL of liquid medicine with different concentrations on prepared artificial feed, uniformly mixing by continuous kneading, placing the uniformly mixed feed in an open-pore culture dish, inoculating test insects, setting 4 repetitions for each treatment, and setting blank control for the test; spodoptera frugiperda larvae at 15 years old per repetition; the Chinese brush is used for touching the larvae for 48 hours after the medicine, the Chinese brush is used for treating the death when the larvae do not respond or have obvious poisoning symptoms (deformity, twitch, feeding stopping and the like), the death number is recorded, the death rate is calculated, the death rate is corrected, DPS software is used as a statistical tool, and the virulence regression equation and LD are calculated 50 。
When the death rate of the blank control is less than 5 percent, correction is not needed, the death rate is between 5 percent and 20 percent, the correction is carried out according to a correction death rate formula, the death rate is more than 20 percent, and the test is carried out again.
Calculating mortality and corrected mortality of each treatment based on the survey data, performing data processing using DPS software, and calculating LD of each medicine 50 、LD 90 Equivalent value of b value (standard error) and LD 50 Is a 95% confidence limit for (c). And the co-toxicity coefficient (CTC value) of the mixture is calculated according to the grand cloud Pei method.
The co-toxicity coefficient (CTC value) of the mixture is calculated according to the formulas (1), (2) and (3):
wherein: ati—actual measured virulence index of the mixture;
LD with S-standard insecticide 50 Milligrams per liter (mg/L);
M-Mixed LD 50 Units are milligrams per liter (mg/L).
TTI＝A×P A +B×P B (2)
Wherein: TTI-the theoretical toxicity index of the mixture;
a-a agent virulence index;
P A the percentage of agent A in the mixture, expressed as a percentage (%);
B-B drug toxicity index;
P B the percentage of agent B in the mixture is expressed as a percentage (%).
Wherein: ctc—co-toxicity coefficient; ati—actual measured virulence index of the mixture; tti—theoretical toxicity index of the mixture.
The co-toxicity coefficient (CTC) of the compound combination is more than or equal to 120, and the synergistic effect is shown; ctc.ltoreq.80 shows antagonism; 80 < CTC < 120 shows additive effect.
E. Test results table 2:
TABLE 2 toxicity measurement results of different proportions of Metroponazole amide and cyantraniliprole
As can be seen from table 2, the concentration of the 2 proportioning agents (oxazachlor: cyantraniliprole=3:1, 7:3, 5:3, 1:1, 3:5, 3:7, 1:3) in the effective inhibition of spodoptera frugiperda is 0.373mg/L, 0.295mg/L, 0.221mg/L, 0.160mg/L, 0.116mg/L, 0.113mg/L, 0.112mg/L, respectively, and the co-toxicity coefficients (CTCs) are 125.52, 133.23, 143.32, 149.57, 165.78, 152.24, 143.51, respectively; it can be seen that the mixture ratio of the oxazine and the cyantraniliprole has a synergistic effect on spodoptera frugiperda.
Test example 2 biological Activity test on liriomyza sativae
The present test was performed with reference to the leaf dipping method proposed by the international commission on resistance activity (IRAC), and the optimum mixing mass ratio of the two insecticides was determined by measuring the effect of the mixture of oxazamate, cyantraniliprole and different mass ratios thereof on liriomyza sativae.
A. Liriomyza sativae (Liriomyza sativae Blanchard) was harvested from suburban cowpea in the Qingdao for 2022 and 9 months; the method comprises the steps of subculturing clean vegetable bean seedlings indoors without any medicament, wherein the raising conditions are that the temperature is 25+/-1 ℃, the relative humidity is 50% -70%, and the photoperiod L:D=16 h:8 h; and (3) placing the potted clean bean seedlings into a net cover with higher density of the liriomyza sativae adults for 24 hours before the test starts, so that the liriomyza sativae adults lay eggs on the bean seedlings. Then, the bean seedlings with the liriomyza sativae eggs are put in a greenhouse at 25 ℃ for breeding, and when the tunnel with the length of about 0.5cm is seen on the bean leaves, the test is carried out.
B. The test agent is 90% oxazine amide and 94% cyantraniliprole original drug
C. The mixing proportion of the oxazine amide and the cyantraniliprole is as follows: the 3:1, 7:3, 5:3, 1:1, 3:5, 3:7, 1:3 formulation concentrations are shown in Table 3:
TABLE 3 dosage ratio of oxazine and cyantraniliprole
D. Toxicity test method
Fresh compound leaves with long handles (leaf collection standard: about 1-3 leaves and 2-year test insects are arranged on each leaf, the length of each insect channel is 0.5 cm-1 cm) generated by American liriomyza sativae larvae are collected from the middle and upper parts of beans by adopting a leaf dipping method proposed by the International Resistance Action Committee (IRAC), and the positions of the test insects on the insect channels are marked by marker pens. And (3) after a proper amount of methanol is preliminarily dissolved in the test medicament raw material, preparing Tween-80 aqueous solution into different concentrations for later use. Soaking the marked leaves in pesticide diluent for 5s, taking out, wrapping the leaf stalks with wet cotton balls, keeping moist, placing into an insect-raising box, and carrying out moisture-keeping raising and observation under the condition of (25+/-1). The treatment was repeated 4 times, the number of test insects was 10 per repetition, and the test results were examined under a binocular dissecting mirror 48 hours after administration. Larval death criteria: the insect body is shriveled and blackened, the insect body is dead, the insect body is fresh and moist, the insect body is naturally prolonged more or the insect body is normal, the insect body is alive, the death number is recorded, the death rate is calculated, the death rate is corrected, DPS software is used as a statistical tool, and the virulence regression equation and LD are calculated 50 。
When the death rate of the blank control is less than 5 percent, correction is not needed, the death rate is between 5 percent and 20 percent, the correction is carried out according to a correction death rate formula, the death rate is more than 20 percent, and the test is carried out again.
Calculating mortality and corrected mortality of each treatment based on the survey data, performing data processing using DPS software, and calculating LD of each medicine 50 、LD 90 Equivalent value of b value (standard error) and LD 50 Is a 95% confidence limit for (c). And the co-toxicity coefficient (CTC value) of the mixture is calculated according to the grand cloud Pei method.
The co-toxicity coefficient (CTC value) of the mixture is calculated according to the formulas (4), (5) and (6):
wherein: ati—actual measured virulence index of the mixture;
LD with S-standard insecticide 50 Milligrams per liter (mg/L);
M-Mixed LD 50 Units are milligrams per liter (mg/L).
TTI＝A×P A +B×P B (5)
Wherein: TTI-the theoretical toxicity index of the mixture;
a-a agent virulence index;
P A the percentage of agent A in the mixture, expressed as a percentage (%);
B-B drug toxicity index;
P B the percentage of agent B in the mixture is expressed as a percentage (%).
Wherein: ctc—co-toxicity coefficient; ati—actual measured virulence index of the mixture; tti—theoretical toxicity index of the mixture.
The co-toxicity coefficient (CTC) of the compound combination is more than or equal to 120, and the synergistic effect is shown; ctc.ltoreq.80 shows antagonism; 80 < CTC < 120 shows additive effect.
E. Test results table 4:
TABLE 4 toxicity measurement results of Metroponazole amide and cyantraniliprole in different ratios
As can be seen from table 4, the concentration of the 7 proportioning agents (oxazachlor: cyantraniliprole=3:1, 7:3, 5:3, 1:1, 3:5, 3:7, 1:3) in the effective inhibition of cowpea liriomyza sativae is 5.67mg/L, 5.54mg/L, 5.36mg/L, 6.12mg/L, 6.83mg/L, 7.79mg/L, 8.54mg/L, respectively, and the co-toxicity coefficients (CTCs) are 138.85, 146.03, 157.46, 148.61, 144.36, 133.30, 126.07, respectively; it can be seen that the mixture ratio of the oxazine and the cyantraniliprole has a synergistic effect on the liriomyza sativae.
Test example 3 Activity test on thrips organisms
The experiment is carried out by referring to NY/T3680-2020 frankliniella occidentalis resistance monitoring technical specification leaf tube film method, and the optimal mixing mass ratio of the two pesticides is screened by measuring the effects of the oxazine amide, the cyantraniliprole and different mass ratio mixtures thereof on cowpea thrips.
A. Cowpea thrips (Frankliniella intonsa Trybom) are collected from cowpea in suburban Qingdao areas; the method is characterized in that no medicament is contacted, and the clean kidney beans are subjected to subculture until now, wherein the culture conditions are that the temperature is 26+/-1 ℃, the relative humidity is 50% -70%, and the photoperiod L:D=14 h:10 h; the feeding method comprises the following steps: two kidney beans are respectively put into a feeding bottle, 40 female adults are selected, the female adults lay eggs, the kidney beans are taken out after 2d and put into another new bottle, and the test is carried out after the kidney beans are uniformly hatched into nymphs of 2 years.
B. The test agent is 90% oxazine amide and 94% cyantraniliprole original drug
C. The mixing proportion of the oxazine amide and the cyantraniliprole is as follows: the 3:1, 7:3, 5:3, 1:1, 3:5, 3:7, 1:3 formulation concentrations are shown in Table 5:
TABLE 5 Metropoxazin and cyantraniliprole dosage ratio
D. Toxicity test method
The reference NY/T3680-2020 frankliniella occidentalis resistance monitoring technical specification leaf tube drug membrane method. Firstly, directly dissolving a test reagent with acetone, then diluting the test reagent with a Tween-80 aqueous solution to a required concentration, and adding the liquid medicine into a centrifuge tube by using a pipette. Pouring out the liquid medicine after shaking for several times upside down, opening the tube cover, and naturally airing on an experiment table; and then heating the needle tip of the insect needle to burn by using an alcohol lamp, and respectively pricking a plurality of small holes on the tube cover and the tube bottom of the 4cm centrifuge tube, wherein the aperture is used for preventing thrips from escaping. Cutting fresh and tender cowpea pods into segments with the length of about 2cm by using a knife, soaking the segments in the tested liquid medicine for 15 seconds, then placing the segments on absorbent paper for natural airing, clamping the segments into a centrifuge tube, sucking 15 first 2-year cowpea thrips nymphs by using a fluke device, and then bouncing the cowpea pods into the centrifuge tube; then the tube is placed in a climatic chamber with the temperature of (26+/-1) DEG C, the lighting setting L: D=14h:10h, the death condition of the test insects is checked after 48h, and the death is considered as the death by the light touch of the small writing brush pen point. Each tube was 1 replicate and each concentration was replicated 4 times. Taking Tween-80 aqueous solution as blank control, recording death number, calculating death rate and correcting death rate, taking DPS software as statistical tool, and calculating virulence regression equation and LD 50 。
When the death rate of the blank control is less than 5 percent, correction is not needed, the death rate is between 5 percent and 20 percent, the correction is carried out according to a correction death rate formula, the death rate is more than 20 percent, and the test is carried out again.
Calculating mortality and corrected mortality of each treatment based on the survey data, performing data processing using DPS software, and calculating LD of each medicine 50 、LD 90 Equivalent value of b value (standard error) and LD 50 Is a 95% confidence limit for (c). And the co-toxicity coefficient (CTC value) of the mixture is calculated according to the grand cloud Pei method.
The co-toxicity coefficient (CTC value) of the mixture is calculated according to the formulas (7), (8) and (9):
wherein: ati—actual measured virulence index of the mixture;
LD with S-standard insecticide 50 Milligrams per liter (mg/L);
M-Mixed LD 50 Units are milligrams per liter (mg/L).
TTI＝A×P A +B×P B (8)
Wherein: TTI-the theoretical toxicity index of the mixture;
a-a agent virulence index;
P A the percentage of agent A in the mixture, expressed as a percentage (%);
B-B drug toxicity index;
P B the percentage of agent B in the mixture is expressed as a percentage (%).
Wherein: ctc—co-toxicity coefficient; ati—actual measured virulence index of the mixture; tti—theoretical toxicity index of the mixture.
The co-toxicity coefficient (CTC) of the compound combination is more than or equal to 120, and the synergistic effect is shown; ctc.ltoreq.80 shows antagonism; 80 < CTC < 120 shows additive effect.
E. Test results table 6:
TABLE 6 toxicity determination results of Metroponazole amide and cyantraniliprole in different ratios
As can be seen from table 6, the concentration of the 7 proportioning agents (oxazachlor: cyantraniliprole=3:1, 7:3, 5:3, 1:1, 3:5, 3:7, 1:3) in the effective inhibition of cowpea thrips is 2.87mg/L, 2.73mg/L, 2.76mg/L, 2.93mg/L, 3.83mg/L, 4.70mg/L, 5.21mg/L, respectively, and the co-toxicity coefficients (CTCs) are 135.18, 148.30, 156.94, 167.32, 147.44, 132.19, 127.78, respectively; the synergistic effect of the mixture ratio of the oxazine amide and the cyantraniliprole on thrips can be seen.
Experimental example 4 field efficacy test for controlling Spodoptera frugiperda
1. Test method
Test agent: the pesticide provided in example 1 and the pesticide provided in comparative examples 1 and 2 were used for controlling spodoptera frugiperda in the field. The test agents are shown in Table 7:
table 7 test dosage meter
The Qingdao city, namely the ink area Guo Guzhuang village (36.437345 north latitude, 120.5631 east longitude, 1.5 mu of test field area, 6.4 pH value of soil and 1.8% of organic matter content), the test field belongs to a northern subtropical monsoon climate area, has sufficient illumination, mild climate, clear four seasons, moderate rainfall, flat topography, good irrigation and drainage conditions and is suitable for carrying out tests.
Sowing conditions: corn seeds are purchased in the market, sowed on 18 days of 06 months of 2022, the mu sowing amount is 4kg, the sowing is performed manually, each hole is 2 grains, the row spacing is 55cm, the hole spacing is 25cm, the mu sowing is about 4800 holes, the thinning or transplanting and seedling supplementing are performed after the corns are aligned, and the management conditions are in accordance with the local agricultural production practice.
Time of application and growth period: the application time is 2022, 07, 28 days,corn is in seedling stage, spodoptera frugiperda is in egg incubation peak stage, and water is applied for water: 600L/hm 2 。
Investigation of control effects: the insect population investigation method comprises the following steps: five samples are taken from each cell, 10 corn plants are randomly investigated at each point, and the number of living insects in the corn plants is peeled. Investigation and recording of the number of insect mouths are carried out before and 3 or 7 days after the application of the pesticide, and the rate of reduction and prevention effect of the insect mouths are calculated.
Drug effect calculation method
And calculating disease index and prevention effect by adopting EXCEL, and performing variance analysis on test data by using a Duncan new complex polar Difference (DMRT) method by using DPS9.5 software.
2. Test results
The control effect of different medicament treatments on lepidoptera pest cordyceps spodoptera is shown in the following table 8.
TABLE 8 control Effect of different agent treatments on Spodoptera frugiperda of corn
As can be seen from table 8, the control effect investigation result shows that: example 1 has a control effect of 88.92% after 3 days of administration when the dosage of the preparation is 10 g/mu, which is extremely superior to the control effects 82.47% and 85.15% of comparative examples 1 and 2; the control effect of the example 1 is 94.22% after 3 days of administration, which is extremely obviously better than the control effects of the comparative examples 1 and 2, namely 85.58% and 89.34%. Through field observation, the test agent and the control agent are safe to corn of test crops, and no phytotoxicity symptoms (such as dwarfing, chlorosis, malformation and the like) are found.
Experimental example 5 field efficacy test for controlling cowpea thrips
1. Test method
Test agent: the pesticide provided in the example 1 and the pesticide provided in the comparative examples 1 and 2 are used for controlling cowpea thrips in the field. The test agents are shown in Table 9 below:
table 9 test dosage meter
Shuzhen Han Zhuang (northern latitude 32.178645, eastern warp 119.167816) in period-tolerant city of Zhenjiang city, jiangsu province, 2 mu of test field area, 6.4 of soil pH value and 1.8% of organic matter content. The test field belongs to a northern subtropical monsoon climate zone, has sufficient illumination, mild climate, distinct four seasons, moderate rainfall, flat topography, good irrigation and drainage conditions and is suitable for carrying out tests.
Sowing conditions: the seeds are purchased in the market, sown in the year 2022 and the month 05 for 25, the plant row spacing is 0.25 x 0.4m, and the cultivation management conditions accord with the local agricultural production practice.
Time of application and growth period: the application time is 2022, 08 and 04 days, cowpea is in flowering phase, thrips are in the initial stage of nymph occurrence, and the application water quantity is as follows: 600L/hm 2 。
Investigation of control effects: investigation of control effect: the Z-shaped five-point sampling method is adopted in the cell, 2 plants are investigated at each point, 10 plants are all investigated, 2 flowers are all investigated at the middle part and the upper part of each plant, 40 flowers are all investigated in each cell, and the living insect number is counted and investigated. Investigation and recording of the number of insect mouths are carried out before and 3 or 7 days after the application of the pesticide, and the rate of reduction and prevention effect of the insect mouths are calculated.
The drug effect calculation method comprises the following steps: and calculating disease index and prevention effect by adopting EXCEL, and performing variance analysis on test data by using a Duncan new complex polar Difference (DMRT) method by using DPS9.5 software.
2. Test results
The control effect of different medicament treatments on cowpea thrips is shown in the following table 10.
TABLE 10 control effects of different agent treatments on cowpea thrips
As can be seen from table 10, the control effect investigation result shows that: example 1 has a control effect of 87.33% after 3 days of administration when the dosage of the preparation is 10 g/mu, which is extremely superior to the control effects of 80.54% and 85.65% of comparative examples 1 and 2; the control effect of the example 1 is 90.76% after 7 days of administration, which is extremely superior to the control effects of the comparative examples 1 and 2, 83.98% and 87.28%. Through field observation, the tested medicament and the control medicament are safe to cowpea of the test crop, and no phytotoxicity symptoms (such as dwarfing, chlorosis, malformation and the like) are found.
Experimental example 6 field efficacy test for controlling liriomyza sativae
1 test method
Test agent: the pesticide provided in example 1 and the pesticide provided in comparative examples 1 and 2 are used for controlling the cowpea liriomyza sativae in the field. The test agents are shown in Table 11 below:
table 11 test dosage for each agent
The Qingdao city, namely Guo Guzhuang village (36.437345 north latitude and 120.5631 east longitude), has a test field area of 2 mu, a soil pH value of 6.4 and an organic matter content of 1.8%. The test field belongs to a northern subtropical monsoon climate zone, has sufficient illumination, mild climate, distinct four seasons, moderate rainfall, flat topography, good irrigation and drainage conditions and is suitable for carrying out tests.
Sowing conditions: the seeds are purchased in the market, sown in the year 2022 and the month 05 and 10, the plant row spacing is 0.25 x 0.4m, and the cultivation management conditions accord with the local agricultural production practice.
Time of application and growth period: the application time is 2022, 08 and 12 days, cowpea is in the flower and fruit stage, liriomyza sativae is in the initial stage of occurrence, and the water amount for application is as follows: 600L/hm 2 。
Investigation of control effects: investigation of control effects: 5 points are marked randomly in each cell, 4 cowpeas with insects are fixed at each point, and 20 cowpeas with insects are all arranged; and marking a point at about lcm on two sides of the front end of each insect channel with insects by using an oily marker pen so that the point and the front end of the insect channel are in the same straight line. During investigation, the body color of the larvae is fresh, full and has eclosion holes according to living insects; the insect body is shrunken and the color is changed according to the dead insect; the presence of a non-identifiable substance is not possible, the insect-viewing channel is prolonged, the insect-viewing channel is eclosion-equipped, and the insect-viewing channel is newly-increased. Investigation and recording of the number of insect mouths are carried out before and 3 or 7 days after the application of the pesticide, and the rate of reduction and prevention effect of the insect mouths are calculated.
The drug effect calculation method comprises the following steps: and calculating disease index and prevention effect by adopting EXCEL, and performing variance analysis on test data by using a Duncan new complex polar Difference (DMRT) method by using DPS9.5 software.
2. Test results
The control effect of different medicament treatments on the cowpea liriomyza sativae is shown in table 12 below.
Table 12 control effects of different agent treatments on liriomyza sativae
As can be seen from the above table, the control effect investigation result shows that: example 1 has the control effect of 87.48% after 3 days of administration when the dosage of the preparation is 10 g/mu, and is extremely superior to the control effects of 80.02% and 84.38% of comparative example 1 and comparative example 2; the control effect of the example 1 is 90.87% after 7 days of administration, which is extremely superior to the control effects of the comparative examples 1 and 2 of 82.47% and 87.59%. Through field observation, the tested medicament and the control medicament are safe to cowpea of the test crop, and no phytotoxicity symptoms (such as dwarfing, chlorosis, malformation and the like) are found.
By now it should be appreciated by those skilled in the art that while a number of exemplary embodiments of the invention have been shown and described herein in detail, many other variations or modifications of the invention consistent with the principles of the invention may be directly ascertained or inferred from the present disclosure without departing from the spirit and scope of the invention. Accordingly, the scope of the present invention should be understood and deemed to cover all such other variations or modifications.
Claims (9)
1. The pesticide insecticide containing the oxazine amide and the cyantraniliprole is characterized by comprising an active component, wherein the active component consists of the oxazine amide and the cyantraniliprole; the mass ratio of the oxazine amide to the cyantraniliprole is 1:30-30:1.
2. A pesticide according to claim 1, wherein the mass ratio of the oxazine amide to the cyantraniliprole is 1:7 to 7:1.
3. A pesticide according to claim 2, wherein the mass ratio of the oxazine amide to the cyantraniliprole is 1:1.
4. A pesticide according to claim 1, characterized in that the ratio of the mass of the oxaziclomefone to the mass of the pesticide is 1-30%.
5. A pesticide according to claim 4, characterized in that the ratio of the mass of the oxaziclomefone to the mass of the pesticide is 10%.
6. A pesticide according to claim 1, characterized in that the ratio of the mass of the cyantraniliprole to the mass of the pesticide is 1-30%.
7. A pesticide according to claim 6, wherein the ratio of the mass of the cyantraniliprole to the mass of the pesticide is 10%.
8. A pesticide according to claim 1, wherein the pesticide is in the form of a liquid formulation.
9. Use of a pesticidal agent according to any one of claims 1 to 8 for controlling spodoptera frugiperda, thrips cowpea and liriomyza sativae.
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