CN117256480A - Control system and control method for industrial automatic production of plant tissues - Google Patents
Control system and control method for industrial automatic production of plant tissues Download PDFInfo
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- CN117256480A CN117256480A CN202311560589.6A CN202311560589A CN117256480A CN 117256480 A CN117256480 A CN 117256480A CN 202311560589 A CN202311560589 A CN 202311560589A CN 117256480 A CN117256480 A CN 117256480A
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- 238000000034 method Methods 0.000 title claims abstract description 49
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 16
- 238000010008 shearing Methods 0.000 claims abstract description 60
- 238000012546 transfer Methods 0.000 claims abstract description 49
- 238000007599 discharging Methods 0.000 claims abstract description 24
- 230000012010 growth Effects 0.000 claims abstract description 13
- 238000011218 seed culture Methods 0.000 claims abstract description 11
- 230000001954 sterilising effect Effects 0.000 claims description 50
- 238000004659 sterilization and disinfection Methods 0.000 claims description 46
- 238000012544 monitoring process Methods 0.000 claims description 32
- 239000007788 liquid Substances 0.000 claims description 24
- 238000012258 culturing Methods 0.000 claims description 14
- 239000002699 waste material Substances 0.000 claims description 13
- 239000010865 sewage Substances 0.000 claims description 8
- 241000894006 Bacteria Species 0.000 abstract description 5
- 238000011109 contamination Methods 0.000 abstract description 4
- 241000196324 Embryophyta Species 0.000 description 112
- 239000000243 solution Substances 0.000 description 54
- 238000004140 cleaning Methods 0.000 description 29
- 230000008569 process Effects 0.000 description 22
- 238000005406 washing Methods 0.000 description 20
- 238000005273 aeration Methods 0.000 description 19
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 15
- 239000002253 acid Substances 0.000 description 14
- 239000003513 alkali Substances 0.000 description 14
- 239000007789 gas Substances 0.000 description 11
- 230000001276 controlling effect Effects 0.000 description 8
- 239000000463 material Substances 0.000 description 8
- 238000009434 installation Methods 0.000 description 6
- 238000004161 plant tissue culture Methods 0.000 description 6
- 230000001105 regulatory effect Effects 0.000 description 6
- 230000001502 supplementing effect Effects 0.000 description 6
- 230000000694 effects Effects 0.000 description 5
- 238000011081 inoculation Methods 0.000 description 4
- 238000005070 sampling Methods 0.000 description 4
- 230000009471 action Effects 0.000 description 3
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 3
- 239000002609 medium Substances 0.000 description 3
- 239000001301 oxygen Substances 0.000 description 3
- 229910052760 oxygen Inorganic materials 0.000 description 3
- 241000208340 Araliaceae Species 0.000 description 2
- 206010020649 Hyperkeratosis Diseases 0.000 description 2
- 235000005035 Panax pseudoginseng ssp. pseudoginseng Nutrition 0.000 description 2
- 235000003140 Panax quinquefolius Nutrition 0.000 description 2
- 230000007547 defect Effects 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 239000006052 feed supplement Substances 0.000 description 2
- 235000008434 ginseng Nutrition 0.000 description 2
- 210000000056 organ Anatomy 0.000 description 2
- 239000002245 particle Substances 0.000 description 2
- 230000002093 peripheral effect Effects 0.000 description 2
- 238000005554 pickling Methods 0.000 description 2
- 239000002912 waste gas Substances 0.000 description 2
- 241001116389 Aloe Species 0.000 description 1
- 241000180649 Panax notoginseng Species 0.000 description 1
- 235000003143 Panax notoginseng Nutrition 0.000 description 1
- 230000001154 acute effect Effects 0.000 description 1
- 235000011399 aloe vera Nutrition 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000000903 blocking effect Effects 0.000 description 1
- 239000000498 cooling water Substances 0.000 description 1
- 238000005520 cutting process Methods 0.000 description 1
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- 230000037213 diet Effects 0.000 description 1
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- 238000010790 dilution Methods 0.000 description 1
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- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
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- 230000005484 gravity Effects 0.000 description 1
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- 208000015181 infectious disease Diseases 0.000 description 1
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- 238000012986 modification Methods 0.000 description 1
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- 230000008635 plant growth Effects 0.000 description 1
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/003—Cutting apparatus specially adapted for tissue culture
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- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Developmental Biology & Embryology (AREA)
- Cell Biology (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a control system and a control method for industrial automatic production of plant tissues, wherein the control system comprises at least two bioreactors which are connected with each other; the seed transferring module is arranged between the bioreactors and used for mutually transferring plant tissues between the bioreactors; the shearing module is arranged on the bioreactor and used for shearing plant tissues in the bioreactor; and the control unit is connected with each module and used for controlling the operation of each module. The bioreactor can automatically perform seed transfer and seed return operations, realizes connection culture, does not contact external environment, and reduces the probability of bacteria contamination; the plant tissues are sheared, so that the plant tissues are prevented from being entangled together to influence the growth speed and the discharging smoothness, and the success rate of seed culture is improved.
Description
Technical Field
The invention belongs to the technical field of plant tissue culture devices, and particularly relates to a control system and a control method for industrial automatic production of plant tissues.
Background
Along with the continuous improvement of living conditions, people generally pay more attention to strengthening physique through medicinal materials with nourishing effect by diet, and perform health care. Due to the huge population, the demand for medicinal plants has risen dramatically. In addition, the cultivation of medicinal plants by the traditional method not only requires a large amount of land and a long growth period, but also requires a proper climate. Any inappropriateness of conditions will limit and decrease the scale of cultivation of the medicinal plants and the yield of the medicinal plants.
Therefore, scientific workers develop a method and a culture device for large-scale cultivation by using isolated tissues or cells of plants. By combining the organs of plants: peeling roots, stems, leaves and the like, then placing the plant into a culture medium containing nutrient components for cultivation, simultaneously providing other environmental conditions such as temperature, illumination and the like suitable for growth, inducing organs of the plant into callus, adventitious buds and adventitious roots, finally, using the callus, the adventitious buds and the adventitious roots as seeds for cultivating the plant, and placing the plant into a cultivation device for cultivation. Furthermore, the defect that the cultivation of the medicinal plants depends on the conditions of land, climate and the like is overcome by culturing the adventitious roots through the culture device, and the yield of the medicinal plants can be improved by large-scale cultivation through a large number of culture devices.
In the prior art, before the seeds are cultured by the culture device, the culture device and the culture solution are required to be sterilized, and oxygen required by the culture is required to be provided in the culture device in the culture process, so that the quality of the seed culture can be seriously affected when the sterilization efficiency is poor or the oxygen is insufficient.
Seeds in the culture device are usually added into the culture device through an inoculation port, and although a sterilization device is usually used for matching inoculation during inoculation, the probability of bacteria infection of the culture device and seeds in the inoculation process is still very high due to limited external environment and conditions, and the quality of seed culture is seriously affected.
When plant tissues are adventitious roots, the growth speed of the adventitious roots in a culture device is much faster than that of original roots, a large amount of adventitious roots can be intertwined with each other after a period of time, even are wound and agglomerated, the production speed and quality of the adventitious roots can be seriously influenced, and the discharge can be unsmooth.
Disclosure of Invention
The technical problem to be solved by the invention is to overcome the defects of the prior art, provide a control system for industrial automation production plant tissues, monitor parameters in a bioreactor through a monitoring module, utilize a control unit to control the operation of each module in the system according to monitoring data, not only realize industrial automation, but also automatically realize seed moving and seed returning operation, and provide new seeds into a seed tank again on the basis of realizing continuous cultivation, the whole process does not need other equipment and does not contact with external environment, thereby not only reducing the probability of bacteria contamination, improving the success rate of seed cultivation, but also reducing the use of equipment, lowering the cost and being convenient to operate.
In order to solve the technical problems, the invention adopts the basic conception of the technical scheme that: a control system for industrial automation of plant tissue, comprising:
at least two interconnected bioreactors;
the seed transferring module is arranged between the bioreactors which are connected with each other and is used for transferring plant tissues between the bioreactors;
the monitoring module is arranged on the bioreactor and extends into the bioreactor and is used for monitoring various parameters in the bioreactor;
the shearing module at least partially stretches into the bioreactor and comprises a shearing part, wherein the shearing part comprises a pin shaft and at least two groups of blade groups which are arranged at intervals in the axial direction of the pin shaft, each group of blade groups comprises a first blade and a second blade which is parallel to the first blade and is arranged in a pasting manner, and the pin shaft vertically penetrates through the centers of the first blade and the second blade, is fixedly connected with the first blade and is rotatably connected with the second blade;
the extension length of the first blade is smaller than that of the second blade, the ends of all the second blades in the blade group in the extension direction are connected together through a connecting piece, and the second blade rotates relative to the first blade to form a notch symmetrical to the pin shaft;
The ends of the first blade and the second blade are respectively bent towards the side where the notch is formed;
and the control unit is connected with each module and controls the operation of the system according to the monitoring data of the monitoring module.
Further, the shearing module comprises a guide cover which is sleeved on the periphery of the shearing part and is in a cylindrical hollow arrangement, an inlet is formed in one end of the guide cover, which faces the bioreactor, an outlet with an extension length is formed in the side wall of the guide cover, and the upper end of the outlet is close to the lower end of the shearing part.
Further, the two interconnected bioreactors include a seed tank for primary culturing of plant tissue and a culture tank for secondary culturing;
the seed transferring module comprises at least one seed transferring pipeline and at least one seed returning pipeline;
the control unit controls the opening of the transplanting pipeline, and transfers the culture solution containing plant tissues in the seed tank into the culture tank;
the control unit controls the opening of the seed returning pipeline to return the partial culture solution containing the plant tissues, which is diluted in the culture tank, to the seed tank.
Further, the monitoring module monitors parameters in the bioreactor to meet the seed transfer requirement, and the control unit controls the shearing module to work to shear plant tissues and controls the opening of a seed transfer pipeline to transfer the sheared plant tissues from the seed tank to the culture tank.
Further, the device comprises an air inlet module which is arranged on the bioreactor and extends into the bioreactor;
the control unit controls the air inlet module to introduce high-temperature steam for sterilization into the bioreactor before culturing or introduce gas for culturing into the bioreactor during culturing, and controls the air inlet speed and/or air inlet amount of the air inlet module according to the parameters monitored by the monitoring module.
Further, the seed feeding device comprises a sterilization module which is respectively connected with the air inlet module, the seed moving pipeline and the seed returning pipeline;
the control unit controls the sterilization module to provide high-temperature steam for sterilization for the air inlet module before cultivation;
the control unit controls the sterilization module to introduce high-temperature steam for sterilization into the seed transfer pipeline before seed transfer or to introduce high-temperature steam for sterilization into the seed return pipeline before seed return.
A control method for industrial automation plant tissue, applied to a control system for industrial automation plant tissue as described above, the method comprising:
s1: adding a culture solution and seeds of plant tissues into a seed tank for primary culture for a certain time to obtain a seed culture solution;
s2: transferring the seed culture solution to a culture tank through a seed transfer pipeline to obtain a diluted seed return solution;
S3: transferring part of the diluted seed returning liquid to a seed tank through a seed returning pipeline for continuous culture;
s4: and (2) after the secondary culture of the residual diluted seed returning liquid in the step (S2) is completed in the culture tank, discharging the mature plant tissues to the next working procedure.
Further, at least steps S1 and S2 include:
controlling to open a sterilization module and a sewage drain channel on the seed transfer pipeline, introducing high-temperature steam into the seed transfer pipeline through the sterilization module, and discharging condensed waste liquid by utilizing the sewage drain channel;
the method comprises the steps of controlling to add a preset volume of culture solution into a culture tank, and controlling to open an air inlet module to introduce a large amount of high-temperature steam which is tiny bubbles into the culture tank; after the sterilization is completed, the culture solution in the culture tank is controlled to be maintained at a proper temperature, and then S2 is executed.
Further, before executing step S2, the method includes:
when the growth state of plant tissues in the seed tank is monitored to reach the preset seed transfer requirement, the shearing module is controlled to work, and the plant tissues are sheared into small sections.
Further, at least steps S2 and S3 include:
and controlling to open a sewage drainage channel on the sterilization module and the seed return pipeline, introducing high-temperature steam into the seed return pipeline through the sterilization module, discharging condensed waste liquid by utilizing the sewage drainage channel, and then executing S3.
Further, step S4 includes: when the growth state of the plant tissues in the culture tank is monitored to reach the preset discharging requirement, the shearing module is controlled to work, the plant tissues are sheared into small sections, then the discharging channel of the culture tank is opened, and the plant tissues are discharged to the next working procedure.
After the technical scheme is adopted, compared with the prior art, the invention has the following beneficial effects:
1. the invention provides a control system for plant tissue in industrial automation production, which monitors parameters in a bioreactor through a monitoring module, utilizes a control unit to control the operation of each module in the system according to monitoring data, not only realizes industrial automation, but also can automatically realize seed moving and seed returning operation, and provides new seeds to a seed tank again on the basis of continuous cultivation, the whole process does not need other equipment and does not contact external environment, thereby not only reducing the probability of bacteria contamination and improving the success rate of seed cultivation, but also reduces the use of equipment and the cost.
2. According to the invention, the shearing module for shearing plant tissues in the bioreactor is arranged, so that the problems of low growth speed, poor quality and unsmooth discharge caused by large size or intertwining and the like of the plant tissues after the plant tissues grow to a certain extent are solved; the shearing part and the guide cover in the shearing module are optimized to meet fixed-length shearing of plant tissues and prevent the plant tissues from being sheared repeatedly.
3. According to the invention, a large amount of high-temperature steam for sterilization or gas for culture in the form of tiny bubbles is provided for the inside of the bioreactor through the air inlet module, so that the contact area of the gas and the culture solution and the dissolution rate of the gas in the culture solution are improved, and the sterilization efficiency is improved; in addition, a large number of small bubbles enter the culture solution, so that the flow of plant tissues in the culture solution is promoted, and the plant tissues are prevented from being damaged due to the fact that the plant tissues are not contacted with gas; the high-temperature steam or gas can be supplied by using the same air inlet module, so that the number of the air inlet modules is reduced, and the cost is reduced.
4. The invention performs sterilization and disinfection treatment on the seed transfer and seed return pipelines through the sterilization module, provides a sterile environment for the seed transfer and seed return processes, and ensures that plant tissues can be smoothly cultured.
The following describes the embodiments of the present invention in further detail with reference to the accompanying drawings.
Drawings
The accompanying drawings, which are included to provide a further understanding of the invention and are incorporated in and constitute a part of this application, illustrate embodiments of the invention and together with the description serve to explain the invention, without limitation to the invention. It is evident that the drawings in the following description are only examples, from which other drawings can be obtained by a person skilled in the art without the inventive effort. In the drawings:
FIG. 1 is a schematic diagram of a control system of the present invention;
FIG. 2 is a schematic diagram of the structure of the culture system according to the present invention;
FIG. 3 is a schematic view of the structure of the shear module according to the present invention;
FIG. 4 is a schematic view of the structure of the shear part in the present invention;
FIG. 5 is a schematic view of the structure of the air intake module of the present invention;
FIG. 6 is a flow chart of the steps of a control method according to an embodiment of the present invention.
In the figure: 100. a seed tank; 200. a culture tank; 1. a seed transferring module; 2. a shearing module; 3. an air intake module; 4. a temperature adjustment module; 5. a cleaning module; 6. a sterilization module; 7. a pressure regulating module; 8. a monitoring module; 9. a material supplementing module; 10. a sampling module; 11. a waste discharging module; 12. a discharging module; 13. a spare seed transferring module; 14. a control unit; 110. a tank bottom valve; 300. a seed transferring pipeline; 310. a seed moving valve; 320. a multi-way valve; 400. CIP cleaning the pipeline; 500. a steam line; 1000. washing the pipeline; 900. a seed returning pipeline; 910. a seed returning valve; 32. a shearing part; 321. a pin shaft; 322. a blade set; 323. a first blade; 324. a second blade; 34. a power section; 35. a guide cover; 351. an inlet; 353. an outlet; 41. an air inlet part; 42. an aeration section; 43. a catheter.
It should be noted that these drawings and the written description are not intended to limit the scope of the inventive concept in any way, but to illustrate the inventive concept to those skilled in the art by referring to the specific embodiments.
Detailed Description
For the purpose of making the objects, technical solutions and advantages of the embodiments of the present invention more apparent, the technical solutions in the embodiments will be clearly and completely described with reference to the accompanying drawings in the embodiments of the present invention, and the following embodiments are used to illustrate the present invention, but are not intended to limit the scope of the present invention.
In the description of the present invention, it should be noted that the positional or positional relationship indicated by the terms such as "upper", "lower", "inner", "outer", "front", "rear", etc. are based on the positional or positional relationship shown in the drawings, are merely for convenience of describing the present invention and simplifying the description, and do not indicate or imply that the apparatus or element in question must have a specific orientation, be constructed and operated in a specific orientation, and thus should not be construed as limiting the present invention.
In the description of the present invention, it should be noted that, unless explicitly specified and limited otherwise, the terms "connected," "abutting," and the like are to be construed broadly, and may be, for example, detachably connected, mechanically connected, or electrically connected; can be directly connected or indirectly connected through an intermediate medium. The specific meaning of the above terms in the present invention will be understood in specific cases by those of ordinary skill in the art.
As an embodiment, the present invention provides a control system for industrial automation production of plant tissue, which may be adventitious roots, plant cells, etc., and the adventitious roots may be adventitious roots of ginseng, adventitious roots of notoginseng, adventitious roots of aloe, etc., in this example, adventitious roots of ginseng are taken as an example.
The control system at least comprises:
at least two interconnected bioreactors;
the seed transferring module 1 is arranged between the bioreactors which are connected with each other and is used for transferring plant tissues between the bioreactors;
the shearing module 2 is arranged on the bioreactor and extends into the bioreactor, and is used for shearing plant tissues in the bioreactor;
the air inlet module 3 is arranged on the bioreactor and extends into the bioreactor, and is used for introducing high-temperature steam for sterilization into the bioreactor before culturing or introducing gas for culturing into the bioreactor in the culturing process;
the temperature adjusting module 4 is arranged on the peripheral wall of the bioreactor and comprises a cavity capable of being filled with a heat transfer medium and is used for adjusting the temperature of the culture solution in the bioreactor; the heat transfer medium comprises cooling water, chilled water, normal-temperature water, high-temperature steam and the like;
The cleaning module 5 is used for carrying out acid/alkali cleaning or water cleaning treatment on the bioreactor and the pipelines in each module;
a sterilization module 6 connected with each module for providing high temperature steam for sterilization to each module;
the pressure regulating module 7 is arranged on the bioreactor and is used for regulating the pressure intensity in the bioreactor;
the monitoring module 8 is arranged on the bioreactor and extends into the bioreactor, and is used for monitoring various parameters in the bioreactor, including a temperature sensor, a pH detection device, a dissolved oxygen amount detection device, a density detector, a liquid level detector, a pressure detector and the like;
a material supplementing module 9 for supplementing materials required by culture, such as culture solution required by culture, plant tissue seeds, acid/alkali solution for maintaining pH value, defoaming solution for eliminating foam generated by culture in a tank, etc. into the bioreactor;
a sampling module 10 for obtaining a plant tissue sample from the bioreactor;
a waste discharge module 11 for discharging waste gas or waste liquid in the bioreactor or pipeline;
a discharging module 12 for discharging the plant tissue reaching the culture requirement in the bioreactor to the next process;
and the control unit 14 is connected with each module and used for controlling the operation of each module so as to enable the whole control system to normally operate.
Valves corresponding to the pipelines are arranged in the modules, the valves are connected with the sterilization module 6 through hoses, and the sterilization module 6 is utilized to sterilize the valves before the valves are used, so that the control system always maintains a sterile culture environment.
In this embodiment, two interconnected bioreactors include a seed tank 100 for primary culturing of plant tissue and a culture tank 200 for secondary culturing.
The seed transfer module 1 comprises at least one seed transfer pipeline 300 and at least one seed return pipeline 900.
The control unit 14 controls the opening of the transplanting line 300 to transfer the culture solution containing plant tissue in the seed tank 100 to the culture tank 200.
The control unit 14 controls the opening of the seed returning line 900 to return the diluted plant tissue-containing portion of the culture solution in the culture tank 200 to the seed tank 100.
The highest point of the seed transfer line 300 is at a level lower than the level at the top of the culture tank 200, and the highest point of the seed returning line 900 is at a level lower than the level at the top of the seed tank 100.
The seed transfer pipeline 300 is equipped with the discharge portion to stretch into the one end of culture tank 200, the one end that returns kind pipeline 900 to stretch into seed tank 100 is equipped with the feed back portion, discharge portion and feed back portion are tubular structure, all incline downwardly extending towards the liquid level direction of culture solution and set up.
The concentration of the culture solution containing plant tissues in the seed transfer line 300 is greater than the concentration of the culture solution containing plant tissues in the seed returning line 900 during seed returning.
In this embodiment, when plant tissue in the seed tank 100 is cultured to a certain extent, the culture solution containing plant tissue in the seed tank 100 is transferred to the culture tank 200 by using the transplanting line 300, and then part of the culture solution containing plant tissue in the culture tank 200 is transferred back to the seed tank 100 by using the reseeding line 900, because the volume of the culture tank 200 is much larger than that of the seed tank 100 and a certain volume of culture solution is added to the culture tank 200 before the transplanting, the concentration of the culture solution containing plant tissue in the transplanting line 300 is larger than that of the culture solution containing plant tissue in the reseeding line 900 during the reseeding; after the plant tissue cultivated in the cultivation tank 200 is matured and discharged, the plant tissue cultivated to a certain extent in the seed tank 100 is again subjected to the cycle operation of transplanting, transplanting and seed returning, thereby realizing the continuous cultivation.
The part of the culture solution containing plant tissues after dilution in the culture tank 200 is returned to the seed tank 100 through the seed returning pipeline 900, so that new seeds are provided to the seed tank 100 again, other equipment is not needed in the whole process, the whole process is not contacted with the external environment, the probability of bacteria contamination is reduced, the success rate of seed culture is improved, meanwhile, the use of equipment is reduced, the cost is reduced, and the operation is convenient.
And, through reducing the setting height of seed shifting pipeline 300 and seed returning pipeline 900, make ejection of compact portion and feed back portion all extend the setting downwards towards the liquid level direction slope of culture solution, be convenient for in follow-up seed shifting and seed returning in-process, reduce the requirement to the pressure differential between seed tank 100 and the culture tank 200, simultaneously, reduce seed shifting and seed returning height, prevent that the culture solution that contains plant tissue from splashing and causing the waste on the tank wall in the transfer in-process, reduce the washing degree of difficulty to seed tank 100 and culture tank 200.
The seed transfer pipeline 300 and the seed returning pipeline 900 are respectively connected with a functional pipeline, the functional pipeline can be alternatively connected with one of the cleaning module 5 and the sterilizing module 6, the cleaning module 5 at least comprises a CIP cleaning pipeline 400 for providing acid/alkali washing liquid and a washing pipeline 1000 for providing washing water, and the sterilizing module 6 at least comprises a steam pipeline 500 for providing high-temperature steam and is used for cleaning, sterilizing and disinfecting the seed transfer pipeline 300 and the seed returning pipeline 900.
The functional pipeline is selectively communicated with the steam pipeline 500, the CIP cleaning pipeline 400 and the water washing pipeline 1000 through four-way valves.
The discharge ports of the seed tank 100 and the culture tank 200 are respectively provided with a tank bottom valve 110.
The seed shifting pipeline 300 is connected with a discharge port of the seed tank 100, a seed shifting valve 310 is arranged on the seed shifting pipeline 300, and the seed shifting valve 310 is matched with a tank bottom valve 110 of the seed tank 100 to open and close the seed shifting pipeline 300.
The seed returning pipeline 900 is connected with a discharge port of the culture tank 200, and a seed returning valve 910 is arranged on the seed returning pipeline; the seed returning valve 910 is opened in cooperation with the bottom valve 110 of the culture tank 200 to close the seed returning line 900.
The seed transfer pipeline 300 and the seed return pipeline 900 are respectively connected with at least a waste discharge module 11 and a discharge module 12 through a multi-way valve 320, and the waste discharge module 11 at least comprises a drain pipeline for discharging cleaning water or steam condensate water and a CIP discharge pipeline for discharging acid/alkali washing liquid.
In another embodiment, the seed tank 100 and the culture tank 200 respectively comprise a tank body, and the bottom wall of the tank body is obliquely arranged to form a cone.
The shearing module 2 is arranged on the bottom wall and extends into the tank body, and is used for intermittently shearing plant tissues in the culture process, so that the production efficiency of the plant tissues is improved, or shearing the plant tissues before transplanting, and preventing the plant tissues with larger sizes from blocking the pipeline during transplanting.
The monitoring module 8 monitors parameters in the bioreactor to meet the seed transfer requirement, and the control unit 14 controls the shearing module 2 to work to shear plant tissues and controls the seed transfer pipeline 300 to be opened to transfer the sheared plant tissues from the seed tank 100 to the culture tank 200.
The shear module 2 comprises at least a shear part 32, a power part 34 and a guide cover 35.
The shearing part 32 is disposed in the bioreactor and comprises a first blade 323 and a second blade 324 which are respectively connected with the power part 34, the first blade 323 and the second blade 324 are parallel and are arranged in a mutually attached manner, and can be driven by the power part 34 to rotate oppositely or reversely to open and close so as to cut plant tissues.
The shearing portion 32 includes a pin 321 connected in series with the first blade 323 and the second blade 324, the first blade 323 is fixedly connected with the pin 321, the second blade 324 is rotatably connected with the pin 321, the second blade 324 rotates relative to the first blade 323 with the pin 321 as a central axis, and a notch is formed by the sides of the first blade 323 and the second blade 324.
The first blade 323 and the second blade 324 have an extension length, the pin 321 is vertically inserted through the centers of the first blade 323 and the second blade 324, and the second blade 324 rotates relative to the first blade 323 to form two symmetrical notches relative to the pin 321.
The ends of the first blade 323 and the second blade 324 are bent toward the side where the notch is formed.
Preferably, the first blade 323 and the second blade 324 have an S shape, and gradually narrow from the center to both ends in the extending direction, and the S-shape directions of the first blade 323 and the second blade 324 are opposite.
In the above scheme, by optimizing the structure and the mounting manner of the first blade 323 and the second blade 324, the second blade 324 rotates relative to the first blade 323 to form two incisions symmetrical relative to the pin shaft 321, and plant tissues are sheared simultaneously through a plurality of incisions, so that the shearing rate is improved; the ends of the first blade 323 and the second blade 324 are respectively bent to one side of the formed incision, so that the formed incision is approximately encircling in the opening and closing process, the plant tissues entering the incision can be gathered, the plant tissues are prevented from being pushed out of the incision by the rotating blades, the plant tissues can be effectively sheared, and the shearing efficiency is improved.
In another connection manner, the shearing portion 32 includes at least two blade groups 322 connected in series to the pin 321, where each blade group 322 is formed by matching the first blade 323 and the second blade 324 in pairs and is distributed at equal intervals along the axis direction of the pin 321, so as to form a multi-layer structure. The spacing between adjacent blade sets 322 is between 1cm and 2cm, preferably between 1cm and 1.5 cm.
Specifically, the extension length of the first blades 323 is smaller than that of the second blades 324, all the first blades 323 in the blade set 322 are parallel to each other and are fixed with the pin shaft 321, all the second blades 324 are parallel to each other and are connected together at the ends in the length direction of the second blades 324 through connecting pieces, and the second blades 324 rotate relative to the first blades 323 to form continuous open and close cuts.
Preferably, when the blade sets 322 are two, two first blades 323 are disposed between two second blades 324 in the axial direction, and the ends of the two second blades 324 are connected by a connecting member to form a frame body covering the outer sides of the first blades 323.
In this way, all the second blades 324 in the blade sets 322 are connected together through the connecting piece, when the second blades 324 are driven to rotate relative to the first blades 323, the second blades 324 in the adjacent blade sets 322 rotate relative to the respective first blades 323 together to form adjacent continuous open and close cuts, so that the plant tissues entering the cutting part 32 can be cut at the same time, the length of the cut plant tissues is consistent with the interval between the adjacent blade sets 322, namely, the plant tissues are cut into small sections with fixed lengths, the growth degree of the plant tissues is convenient to control and monitor, the growth speed is prevented from being influenced by oversized plant tissues, and the pipeline is prevented from being blocked during discharging.
The air guide sleeve 35 is provided as a cylindrical thin-walled tube and is sleeved on the periphery of the shearing part 32.
The connecting piece can be a long-strip connecting rod, can also be a connecting plate with an arc length, can also be a cylindrical connecting cover arranged in a hollow mode, and the like, wherein the axial heights of the connecting rod, the connecting plate and the connecting cover are equal to the distance between the two second blades 324, the radian of the connecting plate is matched with the radian of the air guide cover 35, and the diameter of the connecting cover is smaller than that of the air guide cover 35.
The air guide sleeve 35 is also provided with a cavity which penetrates through two ends along the central axis direction, one end of the air guide sleeve 35 facing the tank body is provided with an inlet 351, and the plane of the inlet 351 is parallel to the plane of the notch formed by the first blade 323 and the second blade 324; the side wall of the pod 35 is also provided with an extended length outlet 353. When the pod 35 is sleeved on the cutout 32, the upper end of the outlet 353 is disposed near the lower end of the cutout 32 in the axial direction.
The inlet 351 directs plant tissue into the cut of the shear module 2 and the outlet 353 is used to discharge sheared plant tissue into the canister.
In the above scheme, the blade in the shearing module 2 rotates to generate vortex suction, plant tissues are sucked into the shearing module 2 from the inlet 351 of the guide cover 35, and the plant tissues are sheared by the blade, so that the plant tissues are sheared into small segments meeting requirements, flow towards the side wall direction of the lower guide cover 35 under the action of gravity and the action of the pushing force of the rotation of the blade, and are discharged from the outlet 353 of the guide cover 35, and the plant tissues are prevented from being sheared repeatedly.
The monitoring module 8 monitors parameters in the bioreactor to meet the seed transfer requirement, and the control unit 14 controls the shearing module 2 to work to shear plant tissues and controls the seed transfer pipeline 300 to be opened to transfer the sheared plant tissues from the seed tank 100 to the culture tank 200. The plant tissues are sheared, so that the plant tissues are prevented from being entangled together to influence the growth speed and the discharging smoothness, and the success rate of seed culture is improved.
In another embodiment, the air intake module 3 is provided on the bottom wall of the bioreactor, comprising an air intake 41, an aeration 42 and a conduit 43.
The air inlet part 41 is alternatively communicated with the sterilization module 6 and the air supply main pipe, and conveys air to the aeration part 42 through a conduit 43, high-temperature steam for sterilization is introduced into the bioreactor before cultivation or gas for cultivation is introduced into the bioreactor in the cultivation process under the control of the control unit 14, and the air inlet speed and/or air inlet amount of the air inlet module 3 are controlled according to the parameters monitored by the monitoring module 8.
The aeration part 42 stretches into the bioreactor, a cavity capable of introducing gas is arranged in the aeration part 42, micropores communicated with the inside and the outside of the cavity are arranged on the cavity wall of the aeration part 42, and a large number of micropores are densely and uniformly distributed on the whole cavity wall.
Preferably, the aeration portion 42 is formed by sintering a stack of a large number of small particles having a diameter of less than 1mm, with gaps between adjacent small particles and micropores having a pore diameter of 1 to 20 μm are formed extending from the inner surface of the cavity wall to the outer surface of the cavity wall.
In another embodiment, at least two air inlet modules 3 and two shearing modules 2 are arranged on the bottom wall of the seed tank 100. The shearing modules 2 and the air inlet modules 3 are alternately distributed at intervals on the bottom wall, and the installation position of the shearing modules 2 is higher than that of the air inlet modules 3 in the vertical direction.
Preferably, the connection line of the installation positions of the two shearing modules 2 and the connection line of the installation positions of the two air inlet modules 3 pass through the center of the seed tank 100 respectively.
The aeration units 42 of the two air inlet modules 3 of the present invention are preferably disposed on the same plane and parallel to each other when mounted, and the aeration units 42 have a certain length, and are disposed so that the middle of the length of the aeration units 42 and the center line of the bottom of the seed tank 100 form a central axis surface and are disposed on both sides of the central axis surface, and a predetermined distance is provided between the aeration units 42 and the shear modules 2 in the direction in which the center of the bottom of the seed tank 100 extends around. The aeration portion 42 adopts this mounting manner, and the uniform distribution of the intake air is sufficiently ensured.
Specifically, one end of the aeration portion 42 has a distance from the bottom wall, and the other end is tangential to or intersects with the central line of the bioreactor, the aeration portion 42 provides gas to enable the culture solution and plant tissues in the bioreactor to rise along the area close to the central line and then diffuse to the peripheral side and circularly move along the outer parabolic track close to the inner wall of the bioreactor, and the culture solution applies a certain force to the plant tissues to enable the plant tissues to be regularly arranged and flow along the length direction along the parabolic track in the rising and falling processes of the culture solution along the track close to the parabolic track;
The shearing module 2 is vertically arranged on the bottom wall, one end with a notch extends towards the central line direction of the bioreactor, a gap is formed between the ends, a shearing plane where the blade is positioned and a track which is close to the parabolic descending form an acute angle, and the culture solution guides regularly arranged plant tissues into the notch in the descending process along the track which is close to the parabolic descending form.
The included angle beta between the shearing module 2 and the bottom wall is adjustable, the aeration portion 42 and the shearing module 2 extend and intersect at a point along the respective length direction to generate an included angle beta 1, the included angle between the bottom wall and the horizontal plane is beta 2, and when the included angle beta satisfies beta 1+ beta 2 = beta, the preferable included angle beta is 90 degrees, the air inlet effect of the aeration portion 42 is optimal.
Likewise, at least four air inlet modules 3 and two shearing modules 2 are provided on the bottom wall of the culture tank 200.
The four air inlet modules 3 are uniformly distributed in the circumferential direction of the bottom wall and are arranged at equal intervals. The aeration portion 42 of the air intake module 3 is kept horizontal and has a certain length. The projections of any two opposing sets of aeration sections 42 in the culture tank 200 are approximately parallel, and the projections of two adjacent sets of aeration sections 42 in the culture tank 200 are approximately perpendicular.
Every two air inlet modules 3 are provided with a shearing module 2 at intervals, and the connecting line of the installation position of one shearing module 2 on the same side of the bottom wall and the installation position of two adjacent air inlet modules 3 in the culture tank 200 approximately forms an isosceles triangle, and the installation position of the shearing module 2 is higher than the air inlet modules 3 in the vertical direction.
Through rationally setting up the relative position of shearing module 2 and air inlet module 3 for air inlet module 3 provides the gas that flows upwards to the inside of bioreactor, and produces decurrent vortex to the inside culture solution of jar, the vortex makes the plant tissue of disorder arrange and carry plant tissue to shearing module 2 department towards same direction, simultaneously, the blade in the shearing module 2 rotates and produces vortex suction, in the import 351 department of follow kuppe 35 is absorbed plant tissue shearing module 2, utilize the blade to cut the processing to the plant tissue of regular arrangement, make plant tissue cut into the minor segment that accords with the requirement, discharge from the export 353 of kuppe 35 again, prevent that plant tissue from agglomerating the winding together, influence the shearing effect, guarantee that the ejection of compact is smooth and easy.
In another embodiment, the plant tissue culture device further comprises a standby seed transferring module 13, wherein the standby seed transferring module 13 is independently arranged and movable, and when the seed transferring module 1 fails and automatic seed transferring and/or seed returning cannot be realized, the plant tissue culture solution can be transferred to the standby seed transferring module 13 through the discharging module 12 for temporary storage, and then the plant tissue culture solution is transferred into the target bioreactor.
The standby seed transferring module 13 has a heat preservation function, plays a role in preserving heat of the culture solution containing plant tissues in the moving process, and ensures the culture quality of the plant tissues.
As one embodiment, the present invention provides a control method for industrially-automated-production plant tissue, which is applied to a control system for industrially-automated-production plant tissue as described above, the method comprising:
s1: adding a culture solution and seeds of plant tissues into a seed tank for primary culture for a certain time to obtain a seed culture solution;
s2: transferring the seed culture solution to a culture tank through a seed transfer pipeline to obtain a diluted seed return solution;
s3: transferring part of the diluted seed returning liquid to a seed tank through a seed returning pipeline for continuous culture;
s4: and (2) after the secondary culture of the residual diluted seed returning liquid in the step (S2) is completed in the culture tank, discharging the mature plant tissues to the next working procedure.
The step S1 is preceded by: and controlling to open the cleaning module to perform acid/alkali cleaning and water cleaning on the seed tank.
The step S1 comprises the following steps: the feed supplementing module is controlled to be opened to add culture solution into the seed tank, the sterilizing module and the air inlet module are opened, and a large amount of high-temperature steam which is tiny bubbles is introduced into the seed tank, so that a sterile culture environment is maintained in the seed tank; the control is opened the feed supplement module and is added plant tissue's seed in to the seed tank, opens temperature regulation module, air inlet module, pressure regulating module, monitoring module again and adjusts the required condition of cultivateing with the culture solution, and the timely feed supplement module of opening according to monitoring data is to the internal required material of supplementary culture of jar in the cultivation process, perhaps opens sampling module, and the cultivation condition is mastered in the sample.
At least the steps between the steps S1 and S2 comprise:
the cleaning module is controlled to be opened to perform acid/alkali cleaning and water cleaning on the seed transfer pipeline, and then the sterilization module is opened to introduce high-temperature steam into the seed transfer pipeline, so that a sterile culture environment is maintained in the seed transfer pipeline;
the cleaning module is controlled to be opened for acid/alkali cleaning and water cleaning of the culture tank, the material supplementing module is controlled to be opened for adding a preset volume of culture solution into the culture tank, the sterilization module and the air inlet module are opened, and a large amount of high-temperature steam which is micro-bubbles is introduced into the culture tank, so that a sterile culture environment is maintained in the culture tank;
the monitoring module monitors that the growth of plant tissues in the seed tank reaches the preset seed transferring requirement, the shearing module is controlled to be opened to shear the plant tissues into small sections, and the pressure regulating module is opened to regulate the pressure of the seed tank and the pressure of the culture tank so as to enable the pressure of the seed tank to meet the seed transferring pressure difference, so that the pressure of the seed tank is larger than the pressure of the culture tank.
The step S2 comprises the following steps: controlling to open a seed transfer pipeline, and transferring the culture solution containing plant tissues into a culture tank; when the monitoring module monitors that the liquid levels in the seed tank and the culture tank reach the preset seed shifting liquid level, the seed shifting pipeline is controlled to be closed, and the seed shifting operation is completed.
At least the steps between the steps S2 and S3 comprise: the cleaning module is controlled to be opened to perform acid/alkali cleaning and water cleaning on the seed returning pipeline, and then the sterilization module is opened to introduce high-temperature steam into the seed returning pipeline, so that a sterile culture environment is maintained in the seed returning pipeline;
And then opening the pressure regulating module to regulate the pressure of the seed tank and the pressure of the culture tank to enable the pressure of the seed tank and the pressure of the culture tank to meet the seed returning pressure difference, so that the pressure of the culture tank is larger than the pressure of the seed tank.
The step S3 comprises the following steps: controlling and opening a seed returning pipeline, and transferring the diluted part of culture solution containing plant tissues into a seed tank; when the monitoring module monitors that the liquid levels in the seed tank and the culture tank reach the preset seed returning liquid level, the seed returning pipeline is controlled to be closed, and seed returning operation is completed;
the plant tissue transferred into the seed pot is used as seed for continuous culture.
Specifically, the cleaning and sterilizing treatment process of the seed returning pipeline comprises the following steps:
when acid/alkali washing is carried out on the seed returning pipeline, the tank bottom valve and the seed returning valve are controlled to be closed, the multi-way valve and the CIP discharge pipeline are opened, then the four-way valve and the valve on the CIP cleaning pipeline are opened, acid/alkali washing liquid is introduced into the seed returning pipeline, the acid/alkali washing liquid flows in the pipeline to be washed, and the acid/alkali washing liquid is discharged from the CIP discharge pipeline;
when the seed returning pipeline is washed, the tank bottom valve and the seed returning valve are controlled to be closed, the multi-way valve and the drain valve are opened, then the four-way valve and the valves on the washing pipeline are opened, washing water is introduced into the seed returning pipeline, the washing water flows in the pipeline for washing, and the washing water is discharged from the drain pipeline;
When the seed returning pipeline is subjected to steam sterilization, the tank bottom valve and the seed returning valve are closed, the multi-way valve and the blow-down valve are opened, then the four-way valve and the valves on the steam pipeline are opened, high-temperature steam is introduced into the seed returning pipeline, the steam diffuses and moves in the pipeline, condenses after contacting with the pipe wall to achieve a sterilization effect, and is discharged from the blow-down pipeline;
after disinfection, the tank bottom valve and the seed returning valve are opened, and other valves are closed, and under the action of pressure difference, the culture solution containing plant tissues flows out from the discharge port and flows into the seed tank through the seed returning pipeline.
When the steam is utilized for sterilization, the multi-way valve and the blow-down valve are opened to realize pressure relief on the seed returning pipeline, so that the steam can be ensured to enter smoothly and continuously, the whole seed returning pipeline is filled, and the sterilization and disinfection effects are ensured.
The process of cleaning and sterilizing the seed transfer line is substantially the same as that of the seed return line and will not be described in detail herein.
The step S4 includes: the temperature adjusting module, the air inlet module, the pressure adjusting module and the monitoring module are opened to adjust the culture solution to the conditions required by culture, and the material supplementing module is opened timely according to the monitoring data to supplement the materials required by culture into the tank body in the culture process, or the sampling module is opened to sample and master the culture condition;
When the growth state of the plant tissues in the culture tank is monitored to reach the preset discharging requirement, the shearing module is controlled to work, the plant tissues are sheared into small sections, the discharging module of the culture tank is opened, and the plant tissues are discharged to the next working procedure.
The above description should be that the preset seed transfer requirement and the preset discharge requirement may be: monitoring the growth length of plant tissues to reach a preset length, or monitoring the time of plant tissue culture to reach a preset time, or monitoring the concentration of plant tissue culture to reach a preset concentration, and the like.
The opening and closing of each module are controlled by the control unit according to preset conditions, for example, when the cleaning time reaches the preset time, the cleaning module is controlled to be closed.
The acid/alkali washing treatment is only carried out when the device is used for the first time, so as to remove burrs and the like in the new device, and only the water washing is carried out in the subsequent culture process, and the acid/alkali washing treatment is not carried out any more; and waste liquid, waste gas and the like generated in the processes of cleaning, sterilizing and the like and the waste materials remained in the seed tank after the seed is transplanted are all discharged from the bioreactor or the pipeline through the waste material discharge module, wherein, because the pickling solution or the alkaline washing solution has a certain acidity and alkalinity, the pickling solution needs to be discharged and treated through a separate pipeline, and the pollution to the environment is prevented.
The seed transferring and seed returning process circulates between the seed tank and the culture tank to realize continuous circulation culture.
After the seed transferring and seed returning operation is completed, the seed transferring pipeline and the seed returning pipeline are required to be cleaned and disinfected again; similarly, the seed tank needs to be cleaned and disinfected again after the seed transfer and the discharge of the culture tank.
The foregoing description is only illustrative of the preferred embodiment of the present invention, and is not to be construed as limiting the invention, but is to be construed as limiting the invention to any simple modification, equivalent variation and variation of the above embodiments according to the technical matter of the present invention without departing from the scope of the invention.
Claims (10)
1. A control system for the industrial automation of plant tissue, comprising:
at least two interconnected bioreactors;
The seed transferring module is arranged between the bioreactors which are connected with each other and is used for transferring plant tissues between the bioreactors;
the monitoring module is arranged on the bioreactor and extends into the bioreactor and is used for monitoring various parameters in the bioreactor;
the shearing module at least partially stretches into the bioreactor and comprises a shearing part, wherein the shearing part comprises a pin shaft and at least two groups of blade groups which are arranged at intervals in the axial direction of the pin shaft, each group of blade groups comprises a first blade and a second blade which is parallel to the first blade and is arranged in a pasting manner, and the pin shaft vertically penetrates through the centers of the first blade and the second blade, is fixedly connected with the first blade and is rotatably connected with the second blade;
the extension length of the first blade is smaller than that of the second blade, the ends of all the second blades in the blade group in the extension direction are connected together through a connecting piece, and the second blade rotates relative to the first blade to form a notch symmetrical to the pin shaft;
the ends of the first blade and the second blade are respectively bent towards the side where the notch is formed;
and the control unit is connected with each module and controls the operation of the system according to the monitoring data of the monitoring module.
2. The control system for the industrial automation production of plant tissue according to claim 1, wherein the shearing module comprises a guide cover which is sleeved on the periphery of the shearing part and is in a cylindrical hollow shape, an inlet is arranged at one end of the guide cover facing the inside of the bioreactor, an outlet with an extension length is arranged on the side wall of the guide cover, and the upper end of the outlet is arranged near the lower end of the shearing part.
3. A control system for the industrial automation of plant tissue production according to claim 2, wherein the two interconnected bioreactors comprise a seed tank for primary cultivation and a cultivation tank for secondary cultivation of plant tissue;
the seed transferring module comprises at least one seed transferring pipeline and at least one seed returning pipeline;
the control unit controls the opening of the transplanting pipeline, and transfers the culture solution containing plant tissues in the seed tank into the culture tank;
the control unit controls the opening of the seed returning pipeline to return the partial culture solution containing the plant tissues, which is diluted in the culture tank, to the seed tank.
4. A control system for the industrial automation of plant tissue production according to claim 3, wherein the monitoring module monitors parameters in the bioreactor to meet the seed transfer requirement, and the control unit controls the shearing module to shear the plant tissue and controls the opening of the seed transfer pipeline to transfer the sheared plant tissue from the seed tank to the culture tank.
5. The control system for the industrial automation of plant tissue production of claim 4, comprising an air intake module disposed on the bioreactor and extending into the interior of the bioreactor;
the control unit controls the air inlet module to introduce high-temperature steam for sterilization into the bioreactor before culturing or introduce gas for culturing into the bioreactor during culturing, and controls the air inlet speed and/or air inlet amount of the air inlet module according to the parameters monitored by the monitoring module.
6. The control system for the industrial automation plant tissue production of claim 5, comprising a sterilization module respectively connected with the air inlet module, the seed transfer pipeline and the seed return pipeline;
the control unit controls the sterilization module to provide high-temperature steam for sterilization for the air inlet module before cultivation;
the control unit controls the sterilization module to introduce high-temperature steam for sterilization into the seed transfer pipeline before seed transfer or to introduce high-temperature steam for sterilization into the seed return pipeline before seed return.
7. A control method for industrial automation plant tissue, characterized by being applied to a control system for industrial automation plant tissue according to any one of claims 1-6, the method comprising:
S1: adding a culture solution and seeds of plant tissues into a seed tank for primary culture for a certain time to obtain a seed culture solution;
s2: transferring the seed culture solution to a culture tank through a seed transfer pipeline to obtain a diluted seed return solution;
s3: transferring part of the diluted seed returning liquid to a seed tank through a seed returning pipeline for continuous culture;
s4: and (2) after the secondary culture of the residual diluted seed returning liquid in the step (S2) is completed in the culture tank, discharging the mature plant tissues to the next working procedure.
8. The method of claim 7, wherein between steps S1 and S2 at least:
controlling to open a sterilization module and a sewage drain channel on the seed transfer pipeline, introducing high-temperature steam into the seed transfer pipeline through the sterilization module, and discharging condensed waste liquid by utilizing the sewage drain channel;
the method comprises the steps of controlling to add a preset volume of culture solution into a culture tank, and controlling to open an air inlet module to introduce a large amount of high-temperature steam which is tiny bubbles into the culture tank; after the sterilization is completed, the culture solution in the culture tank is controlled to be maintained at a proper temperature, and then S2 is executed.
9. The method of claim 7, wherein between steps S2 and S3 at least:
And controlling to open a sewage drainage channel on the sterilization module and the seed return pipeline, introducing high-temperature steam into the seed return pipeline through the sterilization module, discharging condensed waste liquid by utilizing the sewage drainage channel, and then executing S3.
10. A control method for the industrial automation of plant tissue according to any one of claims 7 to 9, wherein step S4 comprises: when the growth state of the plant tissues in the culture tank is monitored to reach the preset discharging requirement, the shearing module is controlled to work, the plant tissues are sheared into small sections, then the discharging channel of the culture tank is opened, and the plant tissues are discharged to the next working procedure.
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| CN102408991A (en) * | 2011-07-28 | 2012-04-11 | 东北林业大学 | Bioreactor system for large scale culture of medicinal plants |
| CN203048953U (en) * | 2013-02-04 | 2013-07-10 | 南京工业大学 | Detachable plant tissue liquid culture apparatus |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102408991A (en) * | 2011-07-28 | 2012-04-11 | 东北林业大学 | Bioreactor system for large scale culture of medicinal plants |
| CN203048953U (en) * | 2013-02-04 | 2013-07-10 | 南京工业大学 | Detachable plant tissue liquid culture apparatus |
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