CN218604427U

CN218604427U - Tissue culture seedling propagation device

Info

Publication number: CN218604427U
Application number: CN202222887627.6U
Authority: CN
Inventors: 黄松波; 张利鹏; 刘瑞瑞; 建明鹏; 王伟; 张晓申; 黄保申; 李国文; 秦亚军; 张广银; 黄保青; 赵洪玲
Original assignee: Henan Yifengnian Biotechnology Co ltd
Current assignee: Henan Yifengnian Biotechnology Co ltd
Priority date: 2022-10-31
Filing date: 2022-10-31
Publication date: 2023-03-14
Anticipated expiration: 2032-10-31

Abstract

The utility model relates to a group banks up seedling technical field's a group and banks up seedling expanding propagation device with earth, including the incubator, the top of incubator is equipped with the loading board, is equipped with a plurality of holes of placing on the loading board, places the nutrition alms bowl in the hole of placing, is equipped with the nutrient solution groove in the incubator of loading board below, and the nutrient solution groove cooperates with each nutrition alms bowl, and the nutrient solution groove communicates with the nutrient solution storage box through the feed liquor pipe, is equipped with the liquid pump on the feed liquor pipe, and the nutrient solution groove is connected with the filter through the back flow, and the liquid outlet of filter is connected with the steriliser through pipeline one, and the liquid outlet of steriliser is connected with the nutrient solution storage box through pipeline two; the top cover of incubator is equipped with transparent safety cover, and the both ends of safety cover are equipped with the bleeder vent. The utility model discloses rational in infrastructure, easy operation, equipment cost is low, saves a large amount of aseptic technique steps, and the technique degree of difficulty is low, and the survival rate is high, and the rate of emergence is high, after the cultivation, the tissue culture seedling can together be takied away with the nutritive cube, when cultivateing a set of seedling of bankeing up with earth, the clearance of being convenient for under the cultivation.

Description

Tissue culture seedling propagation device

Technical Field

The utility model belongs to the technical field of the tissue culture seedling, concretely relates to tissue culture seedling expands numerous device.

Background

At present, tissue culture seedlings are mainly propagated by aseptic tissue culture. The turf vermiculite perlite planted in the seedbed or the plug tray in the greenhouse after being moved out of the tissue culture bottle for seedling hardening and domestication meets the requirements of the substrate, the tissue culture seedlings are subjected to aseptic tissue culture and propagation, and high operation skills, aseptic conditions and a large amount of sterilization and disinfection equipment are required. The link of moving the tissue culture seedlings out of the tissue culture bottle, exercising and domesticating the seedlings requires more accurate control of the environment such as illumination, temperature, humidity and the like, and because the seedlings just coming out of the tissue culture bottle have poor resistance. Higher plant protection insect prevention and disease prevention technologies are needed, the equipment is expensive, the cost is high, the operation is complex, and the technical requirements are high. For example, patent No. CN114747475A discloses a water culture device and method for sweet potato seedling propagation, which comprises a barrel-shaped container, the barrel-shaped container is placed on a horizontal plane, a top cover at the top of the barrel-shaped container is provided with a discharge port, a placing device, a water culture device, a temperature adjusting device and a ventilation device, the temperature can be adjusted along with the development of the sweet potato seedlings in each stage, and the reasonable ventilation of the ventilation device can increase the transpiration of plants, so that the sweet potato seedlings can improve photosynthesis and develop better. In the patent, the root system grows up continuously along with the growth of the seedlings, and the cleaning difficulty is high when the seedlings are reused. Therefore, a tissue culture seedling propagation device is needed to solve the above problems.

SUMMERY OF THE UTILITY MODEL

Aiming at the defects in the prior art, the utility model provides a group banks up seedling and expands numerous device, including the incubator, the top of incubator is equipped with the loading board, be equipped with a plurality of holes of placing on the loading board, a plurality of holes of placing are the matrix arrangement, place the interior nutrition alms bowl that has put of hole, the upper end of nutrition alms bowl is fixed with the round and supports the edge, the external diameter that supports the edge is greater than the diameter of placing the hole, be equipped with the nutrient solution groove in the incubator below the loading board, the nutrient solution groove cooperates with each nutrition alms bowl, the nutrient solution groove is through feed liquor pipe and nutrient solution storage box intercommunication, be equipped with the liquid pump on the feed liquor pipe, the nutrient solution groove is connected with the filter through the back flow, the liquid outlet of filter is connected with the sterilizer through pipeline one, the liquid outlet of sterilizer is connected with the nutrient solution through pipeline two;

the top of the incubator is covered with a transparent protective cover, and two ends of the protective cover are provided with air holes; the incubator is arranged indoors, the indoor environment can be properly sterilized by ozone and ultraviolet lamp sterilization equipment, semi-aseptic virus-free seedling propagation expansion is realized, and virus vector transmission virus diseases can be avoided.

Preferably, the sterilizer comprises a sterilization box, an ultraviolet sterilization lamp is arranged at the top of the sterilization box, a feeding hole of the sterilization box is communicated with the first pipeline, and a discharging hole of the sterilization box is communicated with the second pipeline.

Preferably, be fixed with the connecting block on the lateral wall at the both ends of nutrient solution groove, be equipped with vertical recess on the inner wall of the incubator that the connecting block corresponds, the one end that the nutrient solution groove was kept away from to the connecting block matches and slides and set up in the recess with the recess, be equipped with the threaded rod in the recess, the upper and lower both ends of bearing and recess are passed through at the both ends of threaded rod and are rotated and be connected, and the one end of threaded rod is run through and is connected with positive and negative motor behind the recess.

Preferably, the nutrient solution groove is a transparent groove, and a transparent observation window is arranged on the side wall of the incubator.

Preferably, the protective cover is provided with a door capable of opening and closing, and the protective cover is internally provided with a temperature sensor and a humidity sensor.

Preferably, the protective cover is detachably connected with the incubator.

Preferably, a plurality of fixture blocks are evenly fixed on the outer edge of the top of the incubator, a matched buckle is arranged at the bottom of the protective cover, and the protective cover and the incubator are detachably connected through the buckle and the fixture blocks.

The utility model discloses still including making other subassemblies of a kind of group banks up seedling and expand numerous device normal use, the control assembly of positive reverse motor, temperature sensor's control assembly, humidity transducer's control assembly, the control assembly of ultraviolet ray sterilamp, the control assembly of liquid pump, the control assembly of filter is the conventional technical means in this field. Additionally, the utility model discloses in add the device or the subassembly that limit not all adopt the conventional technical means in this field, if, just reverse motor, liquid pump, filter, humidity transducer, temperature sensor, ultraviolet ray sterilization lamp, nutrition bowl, buckle and fixture block, ozone sterilization etc. all are the conventional equipment in this field.

The utility model discloses a theory of operation: the rapid domestication and propagation expanding device for the tissue culture seedlings is positioned indoors, the indoor environment can be properly sterilized through ozone and ultraviolet lamp sterilization equipment, the propagation expanding of semi-aseptic virus-free seedlings is realized, meanwhile, nutrient solution in the nutrient solution tank continuously and circularly flows, the nutrient solution in the flowing process sequentially passes through the filter and the sterilizer, the filter is used for sterilizing the nutrient solution, and virus diseases can be prevented from being transmitted by virus vectors. The protective cover plays a role in light transmission, ventilation and moisture preservation, and can be matched with artificial light supplement indoors and air-conditioning temperature control, so that indoor annual production is realized. Through the mutual cooperation of the nutrient solution groove, the nutrient solution storage box, the filter and the sterilizer, the tissue culture seedling propagation expansion in a shallow liquid flow nutrient solution supply mode is realized, the aseptic tissue culture propagation expansion is replaced, the nutrient solution which is not absorbed can be recycled, the utilization rate of the nutrient solution is improved, and the waste is reduced. Meanwhile, the distance between the nutrient solution tank and the nutrition pot can be reasonably adjusted according to the growth condition of the seedling, so that the root system of the seedling can be fully contacted with the nutrient solution without extrusion and other conditions, and the seedling culture is facilitated. The nutrition pot can be dismantled with the loading board and be connected, can change the nutrition pot according to the condition, and after the cultivation, the seedling can together take away with the nutrition pot, when cultivateing a set of seedling of banking up next, the clearance of being convenient for. The utility model discloses reached and continued to expand the breed seedling with the fungus and directly transplanted and replaced the domestication link from aseptic group's culture to the cultivation of fungus matrix, increased the volume of emerging, reduced the process, improved the benefit of growing seedlings, the cost is reduced forms the batch production of environment friendly type and grows seedlings.

Low equipment cost, no need of a large number of aseptic operation steps, simple operation, low technical difficulty, high survival rate and high rate of emergence in unit area and unit time.

The beneficial effects of the utility model, rational in infrastructure, easy operation, equipment cost is low, saves a large amount of aseptic operation steps, and the technique degree of difficulty is low, and the survival rate is high, and the rate of emergence is high, cultivates after the end, and the seedling can together tak eoff away with nutritive cube, cultivates when a set of seedling of banking up with earth down, is convenient for clear up.

Drawings

The present invention will be further described with reference to the accompanying drawings and examples.

FIG. 1 is a schematic structural view of a propagation device for tissue culture seedlings according to the present invention;

fig. 2 is an enlarged view at a in fig. 1.

In the figure: 1. an incubator; 2. a protective cover; 3. air holes are formed; 4. tissue culture seedling; 5. a nutrition pot; 6. a nutrient solution tank; 7. a liquid inlet pipe; 8. a liquid pump; 9. a nutrient solution storage tank; 10. a return pipe; 11. a filter; 12. a sterilizer; 13. an ultraviolet sterilizing lamp; 14. connecting blocks; 15. a threaded rod; 16. a positive and negative rotation motor; 17. a groove; 18. a first pipeline; 19. a second pipeline; 20. a carrier plate.

Detailed Description

The present invention will be described more clearly with reference to the accompanying drawings and specific embodiments in the embodiments of the present invention, and the description herein is only for explaining the present invention, but not for limiting the present invention. Based on the embodiments of the present invention, any modifications, equivalent replacements, improvements, etc. made by other embodiments obtained by those skilled in the art without creative efforts should be included in the protection scope of the present invention.

Examples

As shown in fig. 1-2, the utility model provides a propagation device is expanded to tissue culture seedling, including incubator 1, incubator 1's top is equipped with loading board 20, be equipped with a plurality of placing holes on loading board 20, a plurality of placing holes are the matrix arrangement, nutrition bowl 5 has been placed in the placing hole, nutrition bowl 5's upper end is fixed with a round and supports along, the external diameter that supports along is greater than the diameter of placing the hole, be equipped with nutrient solution groove 6 in incubator 1 below loading board 20, nutrient solution groove 6 cooperates with each nutrition bowl 5, nutrient solution groove 6 communicates with nutrient solution storage box 9 through feed liquor pipe 7, be equipped with liquid pump 8 on feed liquor pipe 7, nutrient solution groove 6 is connected with filter 11 through back flow 10, the liquid outlet of filter 11 is connected with sterilizer 12 through pipeline one 18, the liquid outlet of sterilizer 12 is connected with nutrient solution storage box 9 through pipeline two 19;

the top cover of incubator 1 is equipped with transparent safety cover 2, the both ends of safety cover 2 are equipped with bleeder vent 3.

The sterilizer 12 comprises a sterilization box, an ultraviolet sterilization lamp 13 is arranged at the top of the sterilization box, a feeding hole of the sterilization box is communicated with a first pipeline 18, and a discharging hole of the sterilization box is communicated with a second pipeline 19.

Be fixed with connecting block 14 on the lateral wall at the both ends of nutrient solution groove 6, be equipped with vertical recess 17 on the inner wall of incubator 1 that connecting block 14 corresponds, the one end that nutrient solution groove 6 was kept away from to connecting block 14 matches and slides the setting in recess 17 with recess 17, be equipped with threaded rod 15 in the recess 17, the upper and lower both ends of bearing and recess 17 are passed through at the both ends of threaded rod 15 and are rotated and be connected, and the one end of threaded rod 15 is run through and is connected with just reversing motor 16 behind the recess 17.

The nutrient solution groove 6 is a transparent groove, and a transparent observation window is arranged on the side wall of the incubator 1.

Be equipped with the door that can open and shut on the safety cover 2, be equipped with temperature sensor and humidity transducer in the safety cover 2.

The protective cover 2 is detachably connected with the incubator 1.

The outer edge at 1 top of incubator is evenly fixed with a plurality of fixture blocks, the bottom of safety cover 2 is equipped with the complex buckle, safety cover 2 can be dismantled with incubator 1 through buckle and fixture block and be connected. The buckle is connected for prior art with the fixture block, and the repeated description is omitted here, and the buckle structure of the lateral wall that truck railway carriage can open and shut can be referred to specific structure.

When the device is used, the environment where the device is located is disinfected (the tissue culture seedling rapid domestication propagation device is located indoors, the indoor environment can be properly sterilized through ozone and ultraviolet lamp sterilization equipment, semi-aseptic virus-free seedling propagation is realized), plant tissues of sweet potatoes are respectively arranged in nutrition bowls, then the nutrition bowls are placed in the placing holes on the bearing plate, under the effect of the supporting edges of the nutrition bowls, the nutrition bowls can be erected in the placing holes, then the positive and negative rotating motors synchronously rotate, the two threaded rods synchronously rotate to drive the nutrient solution tank to move upwards, and nutrient solution in the nutrient solution tank is contacted with the plant tissues in the nutrition bowls to provide nutrition for the nutrition; finally, a protective cover is covered, and the protective cover plays roles in light transmission, ventilation and moisture preservation; in the process of plant tissue growth, the situation of the plant and the consumption condition of the nutrient solution are observed at any time through an observation window, the nutrient solution is supplemented to a nutrient solution tank through a liquid pump, when a plant root system continuously grows up, a forward and reverse rotating motor can be rotated reversely, the nutrient solution tank is made to descend, sufficient space is provided for the root system, the root system is ensured not to be pressed and damaged when being contacted with the nutrient solution, the propagation of the tissue culture seedling is expanded by adopting a shallow liquid flow nutrient solution supply mode, the growth environment of the plant in a protective cover is timely known through a temperature sensor and a humidity sensor in the whole propagation expanding process, artificial light supplement can be matched, the temperature is controlled by an air conditioner, meanwhile, the nutrient solution in the nutrient solution tank returns to a nutrient solution storage box again after being properly sterilized through a filter and a sterilizer, virus vector propagation virus disease is avoided, the repeated cyclic utilization is realized, the tissue culture seedling is in the best growth environment, and indoor annual production (tissue culture can be carried out all the year round). After the propagation is finished, the protective cover is taken down, the plant after the propagation is taken out, after the cultivation is finished, the tissue culture seedling 4 and the nutrition pot 5 can be taken away together, the nutrition pot can be reused after the tissue culture seedling 4 is taken out, the new nutrition pot can be replaced, the operation is quicker and more convenient, and the cultivation of the next batch of tissue culture seedling 4 is facilitated.

While embodiments of the invention have been described above, the above description is illustrative, not exhaustive, and not limited to the disclosed embodiments. Many modifications and variations will be apparent to those of ordinary skill in the art without departing from the scope and spirit of the illustrated embodiments. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. The utility model provides a tissue culture seedling expands numerous device, includes the incubator, its characterized in that: the top of the incubator is provided with a bearing plate, the bearing plate is provided with a plurality of placing holes, the placing holes are arranged in a matrix, nutrition bowls are placed in the placing holes, a circle of supporting edge is fixed at the upper ends of the nutrition bowls, the outer diameter of the supporting edge is larger than the diameter of the placing holes, a nutrition liquid groove is arranged in the incubator below the bearing plate and matched with each nutrition bowl, the nutrition liquid groove is communicated with a nutrition liquid storage box through a liquid inlet pipe, a liquid pump is arranged on the liquid inlet pipe, the nutrition liquid groove is connected with a filter through a return pipe, the liquid outlet of the filter is connected with a sterilizer through a pipeline I, and the liquid outlet of the sterilizer is connected with the nutrition liquid storage box through a pipeline II;

the top cover of incubator is equipped with transparent safety cover, the both ends of safety cover are equipped with the bleeder vent.

2. The tissue culture seedling propagation device according to claim 1, characterized in that: the sterilizer comprises a sterilization box, an ultraviolet sterilization lamp is arranged at the top of the sterilization box, a feed inlet of the sterilization box is communicated with the first pipeline, and a discharge outlet of the sterilization box is communicated with the second pipeline.

3. The tissue culture seedling propagation device according to claim 1, characterized in that: the utility model discloses a nutrition liquid tank, including nutrient tank, connecting block, bearing and recess, be fixed with the connecting block on the lateral wall at the both ends of nutrient tank, be equipped with vertical recess on the inner wall of the incubator that the connecting block corresponds, the one end that nutrient tank was kept away from to the connecting block matches and slides the setting in the recess with the recess, be equipped with the threaded rod in the recess, the upper and lower both ends of bearing and recess are passed through at the both ends of threaded rod and are rotated and be connected, and the one end of threaded rod is run through and is connected with just reversing motor behind the recess.

4. The tissue culture seedling propagation device according to claim 1, characterized in that: the nutrient solution groove is a transparent groove, and a transparent observation window is arranged on the side wall of the incubator.

5. The tissue culture seedling propagation device according to claim 1, characterized in that: the protective cover is provided with a door capable of opening and closing, and a temperature sensor and a humidity sensor are arranged in the protective cover.

6. The tissue culture seedling propagation device according to claim 1, characterized in that: the safety cover can be dismantled with the incubator and be connected.

7. The tissue culture seedling propagation device according to claim 6, characterized in that: the outer edge at incubator top is evenly fixed with a plurality of fixture blocks, the bottom of safety cover is equipped with the complex buckle, the safety cover passes through the buckle with the incubator and can dismantle with the fixture block and be connected.