CN215872994U - A incubator for plant tissue culture - Google Patents
A incubator for plant tissue culture Download PDFInfo
- Publication number
- CN215872994U CN215872994U CN202121196387.4U CN202121196387U CN215872994U CN 215872994 U CN215872994 U CN 215872994U CN 202121196387 U CN202121196387 U CN 202121196387U CN 215872994 U CN215872994 U CN 215872994U
- Authority
- CN
- China
- Prior art keywords
- hole
- box body
- plant tissue
- tissue culture
- culture
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 238000004161 plant tissue culture Methods 0.000 title claims abstract description 24
- 235000015097 nutrients Nutrition 0.000 claims abstract description 30
- 239000000463 material Substances 0.000 claims abstract description 24
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 16
- 238000005507 spraying Methods 0.000 claims description 13
- 239000000645 desinfectant Substances 0.000 claims description 7
- 238000003780 insertion Methods 0.000 claims description 3
- 230000037431 insertion Effects 0.000 claims description 3
- 240000000111 Saccharum officinarum Species 0.000 abstract description 15
- 235000007201 Saccharum officinarum Nutrition 0.000 abstract description 15
- 241000196324 Embryophyta Species 0.000 abstract description 14
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 abstract description 14
- 229910052760 oxygen Inorganic materials 0.000 abstract description 14
- 239000001301 oxygen Substances 0.000 abstract description 14
- 230000012010 growth Effects 0.000 abstract description 9
- 230000002349 favourable effect Effects 0.000 abstract 1
- 239000011521 glass Substances 0.000 description 6
- 230000035755 proliferation Effects 0.000 description 5
- 239000007788 liquid Substances 0.000 description 4
- 238000009423 ventilation Methods 0.000 description 4
- 241000894006 Bacteria Species 0.000 description 3
- 230000009286 beneficial effect Effects 0.000 description 3
- 238000012258 culturing Methods 0.000 description 3
- 238000001514 detection method Methods 0.000 description 3
- 239000001963 growth medium Substances 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 210000000056 organ Anatomy 0.000 description 3
- 206010020649 Hyperkeratosis Diseases 0.000 description 2
- 238000005273 aeration Methods 0.000 description 2
- 210000004027 cell Anatomy 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 230000008635 plant growth Effects 0.000 description 2
- 210000001938 protoplast Anatomy 0.000 description 2
- 238000007789 sealing Methods 0.000 description 2
- 238000004659 sterilization and disinfection Methods 0.000 description 2
- 239000013589 supplement Substances 0.000 description 2
- 210000001519 tissue Anatomy 0.000 description 2
- 230000002421 anti-septic effect Effects 0.000 description 1
- 230000000903 blocking effect Effects 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000003375 plant hormone Substances 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 238000012827 research and development Methods 0.000 description 1
- 239000012883 rooting culture medium Substances 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
Images
Landscapes
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The utility model relates to the technical field of plant tissue culture devices, and particularly discloses a culture device for plant tissue culture, which comprises a detachable box body and a cover body, wherein a seedling carrying plug tray is placed in the box body, the lower part of the box body is provided with a vent hole and a material passing hole, the vent hole and the material passing hole are respectively connected with a vent pipe and a material passing pipe, and the cover body is provided with a vent hole and a hole cap matched with the vent hole. During the use, the breather pipe can link to each other with the oxygen pump, and the material pipe that leads to can link to each other with nutrient solution case and water pump, can supply or change nutrient solution and adjust the oxygen in the incubator according to the growth needs of plant tissue, more is favorable to plant tissue culture's growth. The setting of year seedling cave dish can cultivate multiunit sugarcane clump seedling simultaneously, and the later stage of growing can also directly carry out the rooting culture through changing the culture solution, need not shift out the plant and carry out the subculture after the branch trunk, further improves plant tissue culture's efficiency.
Description
Technical Field
The utility model belongs to the technical field of plant tissue culture devices, and particularly relates to a culture device for plant tissue culture.
Background
Plant tissue culture, also called isolated culture, refers to a technique of separating desired tissues, organs or cells, protoplasts, etc. from a plant body, and inoculating the isolated tissues, organs or cells, protoplasts, etc. to a culture medium containing various nutrients and plant hormones under aseptic conditions for culture to obtain regenerated whole plants or to produce other products having economic value. The narrow definition refers to the regenerated plant obtained by culturing each part of plant tissue, such as cambium, parenchyma tissue, mesophyll tissue, endosperm, and the like, and also refers to the culture of callus generated from each organ in the culture process, and the callus is redifferentiated to form the regenerated plant. The cultivation of the healthy sugarcane seedlings also adopts aseptic tissue culture. The traditional tissue culture of healthy sugarcane seedlings generally uses a sealed small glass bottle for culture. Namely, a glass bottle is sterilized at high temperature and high pressure, sugarcane tissues are inoculated in the sterilized glass bottle, and then nutrient solution is added for culture. The space of the glass bottle is small, the growth of the sugarcane tissue can be influenced, when the sugarcane tissue is cultured to a certain amount by proliferation, the sugarcane tissue needs to be taken out and divided into a plurality of clusters to be put into different glass bottles for rooting culture, the operation is complicated, and the proliferation efficiency is low.
SUMMERY OF THE UTILITY MODEL
The utility model aims to provide a culture device for plant tissue culture, thereby overcoming the defect that the prior plant tissue culture proliferation culture and rooting culture need separate operations.
In order to achieve the purpose, the utility model provides a culture device for plant tissue culture, which comprises a detachable box body and a cover body, wherein a seedling carrying plug tray is placed in the box body, the lower part of the box body is provided with a vent hole and a material passing hole, the vent hole and the material passing hole are respectively connected with a vent pipe and a material passing pipe, and the cover body is provided with a vent hole and a hole cap matched with the vent hole.
When in use, the vent pipe can be connected with the oxygen pump, and the oxygen pump is started to introduce oxygen into the incubator. The material passing pipe can be connected with the nutrient solution box and the water pump, and the water pump is started to input nutrient solution in the nutrient solution box into the incubator; the nutrient solution in the incubator can also be discharged; the nutrient solution in the incubator can be taken out for detection through the material passing pipe. The seedling-carrying hole tray is placed in the box body of the culture device, the sugarcane propagation seedling cluster buds can be divided into small cluster seedlings which are respectively placed in the holes of the seedling-carrying hole tray for culture, namely, each culture device can simultaneously culture a plurality of groups of sugarcane cluster seedlings, and in the later growth period, the rooting culture can be directly carried out by changing culture solution without removing, dividing and then culturing.
Preferably, the box body and the cover body are detachably connected through a buckle. The specific structure can refer to the structure of a detachable box body on the market.
Preferably, the box body and the cover body are detachably connected through the inserted link and the insertion hole. If the box body is provided with the inserted bar, the cover body is provided with the matched jack, or the box body is provided with the jack, the cover body is provided with the matched inserted bar, or the box body is provided with the jack and the inserted bar, and the cover body is provided with the matched inserted bar and the jack.
Preferably, the box body is provided with a first connecting part, a convex flange is arranged on the inner side of the first connecting part, and a groove-shaped jack is arranged on the first connecting part on the outer side of the flange; the cover body is provided with a second connecting portion corresponding to the first connecting portion, and the second connecting portion is provided with an inserting rod matched with the inserting hole. When box body and lid were fashionable, second connecting portion bottom and first connecting portion contact, the second be connected inboard with the flange contact, simultaneously, in the inserted bar of second connecting portion inserted the jack of first connecting portion, the leakproofness is good, prevents that the bacterium from getting into the box body.
Preferably, the cover body is provided with a spraying device. The spraying device can be installed on the cover body after being purchased from the market. Plant tissues in the box body can be subjected to aeroponic culture according to requirements.
Preferably, the liquid inlet end of the spraying device is connected with at least one of a water tank, a nutrient solution tank or a disinfectant solution tank. And spraying water, nutrient solution or disinfectant into the box body.
Preferably, a temperature sensor is arranged on the cover body. The culture environment in the box body can be monitored in real time.
Preferably, the box body is a cuboid. The cover body is also a cuboid.
Preferably, the box body is 25-35cm long, 18-23 cm wide and 13-15 cm high. The cover may be lower in height than the case.
Preferably, the seedling carrying aperture disk is provided with 8-12 apertures, the diameter of the top of each aperture is 5-6 cm, the diameter of the bottom of each aperture is 2-3 cm, and the depth of each aperture is 5-6 cm.
Compared with the prior art, the utility model has the following beneficial effects:
1. the utility model is provided with the vent hole and the material passing hole at the lower part of the box body, the vent hole and the material passing hole are respectively connected with the vent pipe and the material passing pipe, when in use, the vent pipe can be connected with the oxygen pump, the oxygen pump is started, and oxygen can be introduced into the incubator. The material passing pipe can be connected with the nutrient solution box and the water pump, and the water pump is started to input nutrient solution in the nutrient solution box into the incubator; the nutrient solution in the incubator can also be discharged; the nutrient solution in the incubator can be taken out for detection through the material passing pipe. Namely, the culture device can supplement or replace nutrient solution and adjust the oxygen content in the culture device according to the growth requirement of plant tissues, thereby being more beneficial to the growth of plant tissue culture. Compared with the traditional culture in a glass bottle, the method can reduce the frequency of replacing the nutrient solution and is simpler and more convenient to operate.
2. The seedling-carrying hole disc is placed in the box body of the culture device, the sugarcane propagation seedling cluster buds can be divided into small cluster seedlings to be respectively placed in the holes of the seedling-carrying hole disc for culture, namely, each culture device can simultaneously culture a plurality of groups of sugarcane cluster seedlings, the rooting culture can be directly carried out by changing the culture solution in the later growth period, the plant is not required to be moved out and cultured after the plant is divided, and the efficiency of plant tissue culture is further improved.
3. According to the utility model, the box body is provided with the first connecting part with the convex flange and the groove-shaped jack, the cover body is provided with the second connecting part with the inserted rod, when the box body and the cover body are covered, the bottom of the second connecting part is contacted with the first connecting part, the inner side of the second connecting part is contacted with the flange, and meanwhile, the inserted rod of the second connecting part is inserted into the jack of the first connecting part, so that the sealing property is good, and bacteria are prevented from entering the box body.
Drawings
Fig. 1 is a schematic diagram of a structure according to the present invention.
Fig. 2 is a schematic diagram of the disassembled structure of fig. 1.
Description of the main reference numerals:
1. a box body; 101. a vent hole; 102. a feed through hole; 103. a breather pipe; 104. a material passing pipe; 105. a first connection portion; 106. blocking edges; 107. a jack; 2. a cover body; 201. a second connection; 202. inserting a rod; 203. a ventilation hole; 204. a hole cap; 3. seedling carrying plug trays; 301. a cavity; 4. a spraying device; 5. a temperature sensor.
Detailed Description
The following detailed description of the present invention is provided in conjunction with the accompanying drawings, but it should be understood that the scope of the present invention is not limited to the specific embodiments.
As shown in fig. 1-2, a culture device for plant tissue culture comprises a detachable box body 1 and a cover body 2, wherein a seedling carrying tray 3 is placed in the box body 1, a vent hole 101 and a material passing hole 102 are arranged at the lower part of the box body 1, and the vent hole 101 and the material passing hole 102 are respectively connected with a vent pipe 103 and a material passing pipe 104. When in use, the breather pipe 103 can be connected with an oxygen pump, and the oxygen pump is started to introduce oxygen into the incubator. The material passing pipe 104 can be connected with the nutrient solution box and the water pump, and the water pump is started to input nutrient solution in the nutrient solution box into the incubator; the nutrient solution in the incubator can also be discharged; the nutrient solution in the incubator can be taken out for detection through the material passing pipe 104.
The box body 1 and the cover body 2 are detachably connected through the inserted rod 202 and the inserting hole 107. Specifically, a first connecting part 105 is arranged on the box body 1, a convex rib 106 is arranged on the inner side of the first connecting part 105, and a groove-shaped jack 107 is arranged on the first connecting part 105 on the outer side of the rib 106; the cover 2 is provided with a second connecting portion 201 corresponding to the first connecting portion 105, and the second connecting portion 201 is provided with an inserting rod 202 matched with the inserting hole 107. When the box body 1 and the cover body 2 are closed, the bottom of the second connecting part 201 is in contact with the first connecting part 105, the inner side of the second connecting part 201 is in contact with the flange 106, meanwhile, the inserted rod 202 of the second connecting part 201 is inserted into the insertion hole 107 of the first connecting part 105, the sealing performance is good, and bacteria are prevented from entering the box body 1.
The cover body 2 is provided with a ventilation hole 203 and a hole cap 204 matched with the ventilation hole 203. Exhaust air may be vented through the ventilation holes 203. The cover body 2 is also provided with a spraying device 4. And the liquid inlet end of the spraying device 4 is connected with at least one of the water tank, the nutrient solution tank or the disinfectant tank. Specifically, the liquid inlet pipe of the spraying device 4 can be connected with the branched joint, so that the liquid inlet pipe of the spraying device 4 can be respectively connected with the water tank, the nutrient solution tank and the disinfectant tank, meanwhile, the pipelines for connecting the water tank, the nutrient solution tank and the disinfectant tank are respectively provided with a switch, one of the pipelines is controlled by the switch to be communicated with the spraying device 4, and then water, nutrient solution or disinfectant can be sprayed into the tank. The plant tissue in the box body 1 can be subjected to aeroponics or disinfection according to the requirements. The cover body 2 is also provided with a temperature sensor 5. The culture environment in the box body 1 can be monitored in real time. Temperature sensor 5 can support alarm device, when exceeding the temperature value of settlement, but automatic alarm, research and development personnel can start atomizer 4 as required and spray water or spout the antiseptic solution.
In this embodiment, the box body 1 is a cuboid, and the cover body 2 is also a cuboid. The box body 1 is 25-35cm long, 18-23 cm wide and 13-15 cm high. The cover body 2 is 25-35cm long, 18-23 cm wide and 3-5 cm high. The seedling carrying tray 3 is provided with 8-12 holes 301, the diameter of the top of each hole 301 is 5-6 cm, the diameter of the bottom of each hole 301 is 2-3 cm, and the depth of each hole 301 is 5-6 cm. Of course, in actual production, the sizes of the box body 1, the cover body 2 and the seedling carrying tray 3 can be adjusted according to needs.
The tissue culture of sugarcane is carried out by using the culture device. The box body 1, the cover body 2, the seedling carrying plug 3 and the like are sterilized and disinfected. The aeration pipe 103 is connected with an oxygen pump, and the aeration pipe 104 is connected with a nutrient solution tank and a water pump. At the same time, a growth medium was prepared and sterilized. Small cluster seedlings of sugarcane proliferation cluster buds are placed in the seedling carrying hole tray 3, and a proliferation culture medium is added into the box body 1 through the material through holes 102. After the hole is full of tissue culture seedlings, the sterilized rooting culture medium is added. After the tissue culture seedling takes root, the tissue culture seedling is moved out of the culture device for temporary planting. During the culture period, the spraying device 4 can be started to carry out aeroponics or cooling or disinfection according to the growth condition of the sugarcane seedlings.
In summary, the culture device can supplement or replace nutrient solution and adjust oxygen content in the culture device according to the growth requirement of plant tissues, and is more beneficial to the growth of plant tissue culture. The seedling carrying hole disc 3 can be used for simultaneously culturing multiple groups of sugarcane clump seedlings, and the rooting culture can be directly carried out by changing the culture solution in the later growth period, so that the plants do not need to be moved out and cultured after being divided, and the efficiency of plant tissue culture is further improved.
The foregoing descriptions of specific exemplary embodiments of the present invention have been presented for purposes of illustration and description. It is not intended to limit the utility model to the precise form disclosed, and obviously many modifications and variations are possible in light of the above teaching. The exemplary embodiments were chosen and described in order to explain certain principles of the utility model and its practical application to enable one skilled in the art to make and use various exemplary embodiments of the utility model and various alternatives and modifications as are suited to the particular use contemplated. It is intended that the scope of the utility model be defined by the claims and their equivalents.
Claims (10)
1. The utility model provides a culture apparatus for plant tissue culture, includes detachable box body and lid, its characterized in that, place seedling tray in the box body, the box body lower part is equipped with air vent and logical material hole, air vent and logical material hole link to each other with breather pipe and logical material pipe respectively, be equipped with on the lid the breather hole and with breather hole assorted hole cap.
2. The cultivator for plant tissue culture as claimed in claim 1, wherein the box body and the cover are detachably connected by a buckle.
3. The cultivator for plant tissue culture as claimed in claim 1, wherein the box body and the cover are detachably connected through the inserted link and the insertion hole.
4. The cultivator for plant tissue culture according to claim 3, wherein a first connecting part is arranged on the box body, a convex rib is arranged on the inner side of the first connecting part, and a groove-shaped jack is arranged on the first connecting part on the outer side of the rib; the cover body is provided with a second connecting portion corresponding to the first connecting portion, and the second connecting portion is provided with an inserting rod matched with the inserting hole.
5. The cultivator for plant tissue culture as claimed in claim 1, wherein the cover is provided with a spraying device.
6. The cultivator of claim 5, wherein the inlet end of the spraying device is connected with at least one of a water tank, a nutrient solution tank or a disinfectant solution tank.
7. The cultivator of claim 1, wherein the cover is provided with a temperature sensor.
8. The cultivator for plant tissue culture as claimed in claim 1, wherein the box body is a cuboid.
9. The cultivator of claim 8, wherein the box body is 25-35cm long, 18-23 cm wide and 13-15 cm high.
10. The cultivator for plant tissue culture as claimed in claim 1, wherein the seedling carrying aperture disk has 8-12 holes, the diameter of the top of each hole is 5-6 cm, the diameter of the bottom of each hole is 2-3 cm, and the depth of each hole is 5-6 cm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202121196387.4U CN215872994U (en) | 2021-05-31 | 2021-05-31 | A incubator for plant tissue culture |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202121196387.4U CN215872994U (en) | 2021-05-31 | 2021-05-31 | A incubator for plant tissue culture |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN215872994U true CN215872994U (en) | 2022-02-22 |
Family
ID=80345914
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202121196387.4U Expired - Fee Related CN215872994U (en) | 2021-05-31 | 2021-05-31 | A incubator for plant tissue culture |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN215872994U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113331056A (en) * | 2021-05-31 | 2021-09-03 | 广西壮族自治区农业科学院 | Method for propagating sugarcane tissue culture seedlings |
| CN113331054A (en) * | 2021-05-31 | 2021-09-03 | 广西壮族自治区农业科学院 | Plant tissue culture method by adding culture medium under bacteria environment condition |
-
2021
- 2021-05-31 CN CN202121196387.4U patent/CN215872994U/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113331056A (en) * | 2021-05-31 | 2021-09-03 | 广西壮族自治区农业科学院 | Method for propagating sugarcane tissue culture seedlings |
| CN113331054A (en) * | 2021-05-31 | 2021-09-03 | 广西壮族自治区农业科学院 | Plant tissue culture method by adding culture medium under bacteria environment condition |
| CN113331056B (en) * | 2021-05-31 | 2022-05-27 | 广西壮族自治区农业科学院 | Method for propagating sugarcane tissue culture seedlings |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102150624B (en) | Tissue culture and rapid propagation method of pinellia genus plant | |
| CN215872994U (en) | A incubator for plant tissue culture | |
| CN105638477A (en) | Rapid propagation method for dendrobium hancockii seeds | |
| CN112189566B (en) | Rapid breeding method of cherry seedlings for stocks | |
| CN105993956A (en) | Fast propagating method for atractylis lancea | |
| CN108419679B (en) | Tissue culture method of saffron | |
| CN101124892B (en) | Cymbidium edaphic orchids seed aseptic seeding growing seedlings method | |
| CN1541519A (en) | Paphiopedilum aseptic seeding and tissue culture technology | |
| CN106106147B (en) | A kind of renovation process of lotus unmature subleaf somatic embryo development ways | |
| CN102835310A (en) | Double-tray combined type plant tissue culture container | |
| CN106106178A (en) | A kind of method for tissue culture of confection Rhizoma Iridis Tectori | |
| CN105519448A (en) | Culture method of radix astragali tissue culture seedlings | |
| CN106172008A (en) | A kind of Bowring cattleya fast culture propagation method | |
| CN105766636B (en) | A kind of peony tissue culture regeneration method | |
| CN104686334A (en) | Tissue culture and rapid propagation method for androsace longifolia | |
| CN107318647A (en) | A kind of root-growing agent and its outside sprout-cultivating-bottle method for blueberry tissue culture outside sprout-cultivating-bottle radication | |
| CN1203754C (en) | Breeding technology of test-tube konjac | |
| CN101313661A (en) | Multitudinous transplant method for woody flower group cultivated seedling | |
| CN107853181B (en) | Tobacco explant anti-browning method based on activated carbon | |
| CN108651276A (en) | Rapid Rooting cultural method in blueberry tissue bag | |
| CN110972944B (en) | Method for intermittently immersing dendrobium nobile seedlings | |
| CN102934610A (en) | Breeding maintaining method of ricinus communist female plant | |
| CN107041302A (en) | A kind of culture medium of dendrobium aseptic seeding and sprouting and rooting | |
| CN105557527A (en) | Manufacturing method for artificial cymbidium seeds | |
| CN110679484A (en) | Dendrobium huoshanense seedling cultivation technology |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20220222 |