CN117203325A - 糖苷产物生物合成和回收 - Google Patents

糖苷产物生物合成和回收 Download PDF

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Publication number
CN117203325A
CN117203325A CN202180089758.5A CN202180089758A CN117203325A CN 117203325 A CN117203325 A CN 117203325A CN 202180089758 A CN202180089758 A CN 202180089758A CN 117203325 A CN117203325 A CN 117203325A
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CN
China
Prior art keywords
seq
bacterial cell
enzyme
ugt
glycosylation
Prior art date
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Pending
Application number
CN202180089758.5A
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English (en)
Chinese (zh)
Inventor
阿吉库马尔·帕拉伊尔·库马兰
克里斯汀·妮可·S·桑托斯
詹森·唐纳德
亚伦·洛夫
郑毅英
阿德尔·加德里
维尼特·塞斯崔
陈璐
克里斯托弗·图米
汉娜·林奇
埃里克·尼米宁
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Manus Biosynthesis Inc
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Manus Biosynthesis Inc
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Filing date
Publication date
Application filed by Manus Biosynthesis Inc filed Critical Manus Biosynthesis Inc
Publication of CN117203325A publication Critical patent/CN117203325A/zh
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/44—Preparation of O-glycosides, e.g. glucosides
    • C12P19/60—Preparation of O-glycosides, e.g. glucosides having an oxygen of the saccharide radical directly bound to a non-saccharide heterocyclic ring or a condensed ring system containing a non-saccharide heterocyclic ring, e.g. coumermycin, novobiocin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/44—Preparation of O-glycosides, e.g. glucosides
    • C12P19/56—Preparation of O-glycosides, e.g. glucosides having an oxygen atom of the saccharide radical directly bound to a condensed ring system having three or more carbocyclic rings, e.g. daunomycin, adriamycin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C12N9/1062—Sucrose synthase (2.4.1.13)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/18—Preparation of compounds containing saccharide radicals produced by the action of a glycosyl transferase, e.g. alpha-, beta- or gamma-cyclodextrins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • C12Y204/01017—Glucuronosyltransferase (2.4.1.17)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y503/00—Intramolecular oxidoreductases (5.3)
    • C12Y503/01—Intramolecular oxidoreductases (5.3) interconverting aldoses and ketoses (5.3.1)
    • C12Y503/01009—Glucose-6-phosphate isomerase (5.3.1.9)

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Saccharide Compounds (AREA)
CN202180089758.5A 2020-11-24 2021-11-24 糖苷产物生物合成和回收 Pending CN117203325A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US202063117534P 2020-11-24 2020-11-24
US63/117,534 2020-11-24
PCT/US2021/060722 WO2022115527A1 (en) 2020-11-24 2021-11-24 Glycoside product biosynthesis and recovery

Publications (1)

Publication Number Publication Date
CN117203325A true CN117203325A (zh) 2023-12-08

Family

ID=81755020

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202180089758.5A Pending CN117203325A (zh) 2020-11-24 2021-11-24 糖苷产物生物合成和回收

Country Status (9)

Country Link
US (1) US20240093258A1 (https=)
EP (1) EP4251731A4 (https=)
JP (1) JP2023550496A (https=)
KR (1) KR20230112673A (https=)
CN (1) CN117203325A (https=)
AU (1) AU2021386386A1 (https=)
CA (1) CA3200689A1 (https=)
MX (1) MX2023006019A (https=)
WO (1) WO2022115527A1 (https=)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN119931981A (zh) * 2024-05-29 2025-05-06 东台市浩瑞生物科技有限公司 一种糖基转移酶ugt76g1突变体及其催化合成莱鲍迪苷a的方法

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113302298B (zh) 2018-11-09 2025-02-25 银杏生物制品公司 罗汉果甙的生物合成
WO2024121721A1 (en) 2022-12-05 2024-06-13 Tate & Lyle Solutions Usa Llc Methods for obtaining steviol glycosides
WO2026063396A1 (ja) * 2024-09-20 2026-03-26 富士フイルム株式会社 変異型スクロースシンターゼ、変異型スクロースシンターゼをコードする核酸、発現ベクター、形質転換体、変異型スクロースシンターゼの製造方法、変異型スクロースシンターゼを含む組成物、ウリジン二リン酸－グルコースの製造方法、糖化合物の製造方法

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7989677B2 (en) * 2005-08-17 2011-08-02 Nestec S. A. Nucleic acids and proteins associated with sucrose accumulation in coffee
CN108396044A (zh) 2011-08-08 2018-08-14 埃沃尔瓦公司 甜菊醇糖苷类的重组生产
AU2013211605B2 (en) * 2012-01-23 2017-04-06 Dsm Ip Assets B.V. Diterpene production
EP3215629B1 (en) * 2014-11-05 2024-08-07 Manus Bio Inc. Microbial production of steviol glycosides
AU2019304965B2 (en) * 2018-07-16 2025-04-24 Manus Bio, Inc. Production of steviol glycosides through whole cell biotransformation
MX2021005219A (es) * 2018-11-07 2021-06-18 Firmenich Incorporated Metodos para hacer edulcorantes de alta intensidad.
MX2021006986A (es) * 2018-12-12 2021-08-16 Conagen Inc Produccion biosintetica de variantes de glucosidos de esteviol.

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN119931981A (zh) * 2024-05-29 2025-05-06 东台市浩瑞生物科技有限公司 一种糖基转移酶ugt76g1突变体及其催化合成莱鲍迪苷a的方法

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Publication number Publication date
AU2021386386A1 (en) 2023-06-22
EP4251731A1 (en) 2023-10-04
JP2023550496A (ja) 2023-12-01
KR20230112673A (ko) 2023-07-27
CA3200689A1 (en) 2022-06-02
US20240093258A1 (en) 2024-03-21
EP4251731A4 (en) 2025-02-12
WO2022115527A1 (en) 2022-06-02
MX2023006019A (es) 2023-08-04

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