JP2023550496A - グリコシド生成物の生合成及び回収 - Google Patents

グリコシド生成物の生合成及び回収 Download PDF

Info

Publication number
JP2023550496A
JP2023550496A JP2023530874A JP2023530874A JP2023550496A JP 2023550496 A JP2023550496 A JP 2023550496A JP 2023530874 A JP2023530874 A JP 2023530874A JP 2023530874 A JP2023530874 A JP 2023530874A JP 2023550496 A JP2023550496 A JP 2023550496A
Authority
JP
Japan
Prior art keywords
seq
enzyme
bacterial cell
ugt
glycosylation
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP2023530874A
Other languages
English (en)
Japanese (ja)
Other versions
JP2023550496A5 (https=
Inventor
アジクマー パライル クマラン
クリスティーン ニコル エス． サントス
ジェイソン ドナルド
アーロン ラブ
イーイン ジャン
アデル ガーデリ
ヴィニート シャストリー
ルー チェン
クリストファー トゥーミー
ハンナ リンチ
エリック ニエミネン
Original Assignee
マナス バイオ インコーポレイテッド
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by マナス バイオ インコーポレイテッド filed Critical マナス バイオ インコーポレイテッド
Publication of JP2023550496A publication Critical patent/JP2023550496A/ja
Publication of JP2023550496A5 publication Critical patent/JP2023550496A5/ja
Pending legal-status Critical Current

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/44—Preparation of O-glycosides, e.g. glucosides
    • C12P19/60—Preparation of O-glycosides, e.g. glucosides having an oxygen of the saccharide radical directly bound to a non-saccharide heterocyclic ring or a condensed ring system containing a non-saccharide heterocyclic ring, e.g. coumermycin, novobiocin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/44—Preparation of O-glycosides, e.g. glucosides
    • C12P19/56—Preparation of O-glycosides, e.g. glucosides having an oxygen atom of the saccharide radical directly bound to a condensed ring system having three or more carbocyclic rings, e.g. daunomycin, adriamycin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C12N9/1062—Sucrose synthase (2.4.1.13)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/18—Preparation of compounds containing saccharide radicals produced by the action of a glycosyl transferase, e.g. alpha-, beta- or gamma-cyclodextrins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • C12Y204/01017—Glucuronosyltransferase (2.4.1.17)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y503/00—Intramolecular oxidoreductases (5.3)
    • C12Y503/01—Intramolecular oxidoreductases (5.3) interconverting aldoses and ketoses (5.3.1)
    • C12Y503/01009—Glucose-6-phosphate isomerase (5.3.1.9)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Saccharide Compounds (AREA)
JP2023530874A 2020-11-24 2021-11-24 グリコシド生成物の生合成及び回収 Pending JP2023550496A (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US202063117534P 2020-11-24 2020-11-24
US63/117,534 2020-11-24
PCT/US2021/060722 WO2022115527A1 (en) 2020-11-24 2021-11-24 Glycoside product biosynthesis and recovery

Publications (2)

Publication Number Publication Date
JP2023550496A true JP2023550496A (ja) 2023-12-01
JP2023550496A5 JP2023550496A5 (https=) 2024-11-29

Family

ID=81755020

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2023530874A Pending JP2023550496A (ja) 2020-11-24 2021-11-24 グリコシド生成物の生合成及び回収

Country Status (9)

Country Link
US (1) US20240093258A1 (https=)
EP (1) EP4251731A4 (https=)
JP (1) JP2023550496A (https=)
KR (1) KR20230112673A (https=)
CN (1) CN117203325A (https=)
AU (1) AU2021386386A1 (https=)
CA (1) CA3200689A1 (https=)
MX (1) MX2023006019A (https=)
WO (1) WO2022115527A1 (https=)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2026063396A1 (ja) * 2024-09-20 2026-03-26 富士フイルム株式会社 変異型スクロースシンターゼ、変異型スクロースシンターゼをコードする核酸、発現ベクター、形質転換体、変異型スクロースシンターゼの製造方法、変異型スクロースシンターゼを含む組成物、ウリジン二リン酸－グルコースの製造方法、糖化合物の製造方法

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113302298B (zh) 2018-11-09 2025-02-25 银杏生物制品公司 罗汉果甙的生物合成
WO2024121721A1 (en) 2022-12-05 2024-06-13 Tate & Lyle Solutions Usa Llc Methods for obtaining steviol glycosides

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20090055974A1 (en) * 2005-08-17 2009-02-26 Cornell University Nucleic acids and proteins associated with sucrose accumulation in coffee
US20150031868A1 (en) * 2012-01-23 2015-01-29 Dsm Ip Assets B.V. Diterpene production
JP2017532976A (ja) * 2014-11-05 2017-11-09 マナス バイオシンセシス インコーポレイテッド ステビオール配糖体の微生物による生成
WO2020018506A2 (en) * 2018-07-16 2020-01-23 Manus Bio, Inc. Production of steviol glycosides through whole cell biotransformation
WO2020096907A1 (en) * 2018-11-07 2020-05-14 Firmenich Incorporated Methods for making high intensity sweeteners
WO2020123877A1 (en) * 2018-12-12 2020-06-18 Conagen Inc. Biosynthetic production of variant steviol glycosides

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108396044A (zh) 2011-08-08 2018-08-14 埃沃尔瓦公司 甜菊醇糖苷类的重组生产

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20090055974A1 (en) * 2005-08-17 2009-02-26 Cornell University Nucleic acids and proteins associated with sucrose accumulation in coffee
US20150031868A1 (en) * 2012-01-23 2015-01-29 Dsm Ip Assets B.V. Diterpene production
JP2017532976A (ja) * 2014-11-05 2017-11-09 マナス バイオシンセシス インコーポレイテッド ステビオール配糖体の微生物による生成
WO2020018506A2 (en) * 2018-07-16 2020-01-23 Manus Bio, Inc. Production of steviol glycosides through whole cell biotransformation
WO2020096907A1 (en) * 2018-11-07 2020-05-14 Firmenich Incorporated Methods for making high intensity sweeteners
WO2020123877A1 (en) * 2018-12-12 2020-06-18 Conagen Inc. Biosynthetic production of variant steviol glycosides

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2026063396A1 (ja) * 2024-09-20 2026-03-26 富士フイルム株式会社 変異型スクロースシンターゼ、変異型スクロースシンターゼをコードする核酸、発現ベクター、形質転換体、変異型スクロースシンターゼの製造方法、変異型スクロースシンターゼを含む組成物、ウリジン二リン酸－グルコースの製造方法、糖化合物の製造方法

Also Published As

Publication number Publication date
AU2021386386A1 (en) 2023-06-22
EP4251731A1 (en) 2023-10-04
CN117203325A (zh) 2023-12-08
KR20230112673A (ko) 2023-07-27
CA3200689A1 (en) 2022-06-02
US20240093258A1 (en) 2024-03-21
EP4251731A4 (en) 2025-02-12
WO2022115527A1 (en) 2022-06-02
MX2023006019A (es) 2023-08-04

Similar Documents

Publication Publication Date Title
US12012626B2 (en) Production of steviol glycosides through whole cell biotransformation of steviol glycoside intermediates
US11788070B2 (en) Uridine diphosphate-dependent glycosyltransferase enzyme
JP2021052788A (ja) ステビオール配糖体の分離、単離及び評価のための方法
US11098315B2 (en) Biosynthetic production of steviol glycosides and processes therefore
JP2023550496A (ja) グリコシド生成物の生合成及び回収
CN109563118A (zh) 甜菊醇糖苷的结晶
CN107820394A (zh) 甜菊醇糖苷组合物
CN107849081A (zh) 甜菊醇糖苷组合物
KR101199821B1 (ko) 당 전이 효소를 이용한 기능성 천연 고감미 올리고 배당체 제조
CN109563117A (zh) 甜菊醇糖苷的结晶
WO2024228615A1 (en) High-purity steviol glycosides
CN119487193A (zh) 甜菊醇糖苷生物合成变异酶与其利用

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20241121

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20241121

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20260127

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20260427