CN117126016A - Preparation and application methods of plant nutrition conditioner based on marine oligomeric amino polysaccharide - Google Patents
Preparation and application methods of plant nutrition conditioner based on marine oligomeric amino polysaccharide Download PDFInfo
- Publication number
- CN117126016A CN117126016A CN202310874403.8A CN202310874403A CN117126016A CN 117126016 A CN117126016 A CN 117126016A CN 202310874403 A CN202310874403 A CN 202310874403A CN 117126016 A CN117126016 A CN 117126016A
- Authority
- CN
- China
- Prior art keywords
- marine
- parts
- plant nutrition
- oligomeric
- conditioner
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 230000001863 plant nutrition Effects 0.000 title claims abstract description 46
- 229920001282 polysaccharide Polymers 0.000 title claims abstract description 32
- 239000005017 polysaccharide Substances 0.000 title claims abstract description 32
- -1 amino polysaccharide Chemical class 0.000 title claims abstract description 26
- 238000000034 method Methods 0.000 title claims abstract description 19
- 238000002360 preparation method Methods 0.000 title abstract description 7
- 235000005035 Panax pseudoginseng ssp. pseudoginseng Nutrition 0.000 claims abstract description 31
- 235000003140 Panax quinquefolius Nutrition 0.000 claims abstract description 31
- 235000008434 ginseng Nutrition 0.000 claims abstract description 31
- 239000003337 fertilizer Substances 0.000 claims abstract description 13
- WZLMXYBCAZZIRQ-UHFFFAOYSA-N [N].[P].[K] Chemical compound [N].[P].[K] WZLMXYBCAZZIRQ-UHFFFAOYSA-N 0.000 claims abstract description 12
- QJZYHAIUNVAGQP-UHFFFAOYSA-N 3-nitrobicyclo[2.2.1]hept-5-ene-2,3-dicarboxylic acid Chemical compound C1C2C=CC1C(C(=O)O)C2(C(O)=O)[N+]([O-])=O QJZYHAIUNVAGQP-UHFFFAOYSA-N 0.000 claims abstract description 10
- 239000004021 humic acid Substances 0.000 claims abstract description 10
- 239000000843 powder Substances 0.000 claims abstract description 6
- 244000046052 Phaseolus vulgaris Species 0.000 claims abstract description 4
- 235000010627 Phaseolus vulgaris Nutrition 0.000 claims abstract description 4
- 241000196324 Embryophyta Species 0.000 claims description 12
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 9
- 238000005469 granulation Methods 0.000 claims description 9
- 230000003179 granulation Effects 0.000 claims description 9
- 235000015097 nutrients Nutrition 0.000 claims description 9
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 claims description 8
- 229910052700 potassium Inorganic materials 0.000 claims description 8
- 239000011591 potassium Substances 0.000 claims description 8
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 claims description 6
- 229910052698 phosphorus Inorganic materials 0.000 claims description 6
- 239000011574 phosphorus Substances 0.000 claims description 6
- 235000019764 Soybean Meal Nutrition 0.000 claims description 5
- 239000002245 particle Substances 0.000 claims description 5
- 239000004455 soybean meal Substances 0.000 claims description 5
- 229910052757 nitrogen Inorganic materials 0.000 claims description 4
- 239000005416 organic matter Substances 0.000 claims description 4
- 235000021049 nutrient content Nutrition 0.000 claims description 2
- 244000131316 Panax pseudoginseng Species 0.000 claims 1
- 239000002689 soil Substances 0.000 abstract description 34
- 241000208340 Araliaceae Species 0.000 abstract description 30
- 230000009286 beneficial effect Effects 0.000 abstract description 8
- 244000005700 microbiome Species 0.000 abstract description 7
- 239000003795 chemical substances by application Substances 0.000 abstract description 6
- 230000003750 conditioning effect Effects 0.000 abstract description 6
- 230000000694 effects Effects 0.000 abstract description 6
- 238000009825 accumulation Methods 0.000 abstract description 5
- 238000003860 storage Methods 0.000 abstract description 5
- 230000001737 promoting effect Effects 0.000 abstract description 2
- 230000000813 microbial effect Effects 0.000 description 10
- 230000000052 comparative effect Effects 0.000 description 8
- 238000004458 analytical method Methods 0.000 description 6
- 150000004676 glycans Chemical class 0.000 description 6
- 239000000126 substance Substances 0.000 description 6
- 238000011282 treatment Methods 0.000 description 5
- 241000233866 Fungi Species 0.000 description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
- 241000894006 Bacteria Species 0.000 description 3
- 241000566145 Otus Species 0.000 description 3
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- MMDJDBSEMBIJBB-UHFFFAOYSA-N [O-][N+]([O-])=O.[O-][N+]([O-])=O.[O-][N+]([O-])=O.[NH6+3] Chemical compound [O-][N+]([O-])=O.[O-][N+]([O-])=O.[O-][N+]([O-])=O.[NH6+3] MMDJDBSEMBIJBB-UHFFFAOYSA-N 0.000 description 3
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 description 3
- 230000001580 bacterial effect Effects 0.000 description 3
- 230000008859 change Effects 0.000 description 3
- 238000012364 cultivation method Methods 0.000 description 3
- 238000000605 extraction Methods 0.000 description 3
- 238000012165 high-throughput sequencing Methods 0.000 description 3
- 238000012986 modification Methods 0.000 description 3
- 230000004048 modification Effects 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 150000003384 small molecules Chemical class 0.000 description 3
- 241000894007 species Species 0.000 description 3
- 241000235349 Ascomycota Species 0.000 description 2
- 241000221198 Basidiomycota Species 0.000 description 2
- 241000192142 Proteobacteria Species 0.000 description 2
- 238000005904 alkaline hydrolysis reaction Methods 0.000 description 2
- 230000003321 amplification Effects 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 230000007613 environmental effect Effects 0.000 description 2
- 230000002538 fungal effect Effects 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 238000002156 mixing Methods 0.000 description 2
- 238000003199 nucleic acid amplification method Methods 0.000 description 2
- 239000003895 organic fertilizer Substances 0.000 description 2
- 150000003839 salts Chemical class 0.000 description 2
- 238000012163 sequencing technique Methods 0.000 description 2
- 230000002195 synergetic effect Effects 0.000 description 2
- 108020004465 16S ribosomal RNA Proteins 0.000 description 1
- 241000186361 Actinobacteria <class> Species 0.000 description 1
- 241000193830 Bacillus <bacterium> Species 0.000 description 1
- 102100031974 CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,3-sialyltransferase 4 Human genes 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 241000233652 Chytridiomycota Species 0.000 description 1
- 238000007400 DNA extraction Methods 0.000 description 1
- 238000001712 DNA sequencing Methods 0.000 description 1
- 102100034274 Diamine acetyltransferase 1 Human genes 0.000 description 1
- 101000703754 Homo sapiens CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,3-sialyltransferase 4 Proteins 0.000 description 1
- 101000641077 Homo sapiens Diamine acetyltransferase 1 Proteins 0.000 description 1
- 101000713305 Homo sapiens Sodium-coupled neutral amino acid transporter 1 Proteins 0.000 description 1
- 101000640813 Homo sapiens Sodium-coupled neutral amino acid transporter 2 Proteins 0.000 description 1
- 101000716973 Homo sapiens Thialysine N-epsilon-acetyltransferase Proteins 0.000 description 1
- 241000235575 Mortierella Species 0.000 description 1
- 238000012408 PCR amplification Methods 0.000 description 1
- 241000235527 Rhizopus Species 0.000 description 1
- 101150077669 SAT4 gene Proteins 0.000 description 1
- 101100450131 Schizosaccharomyces pombe (strain 972 / ATCC 24843) hal4 gene Proteins 0.000 description 1
- 238000012352 Spearman correlation analysis Methods 0.000 description 1
- 102100020926 Thialysine N-epsilon-acetyltransferase Human genes 0.000 description 1
- CKUAXEQHGKSLHN-UHFFFAOYSA-N [C].[N] Chemical compound [C].[N] CKUAXEQHGKSLHN-UHFFFAOYSA-N 0.000 description 1
- RDXARWSSOJYNLI-UHFFFAOYSA-N [P].[K] Chemical compound [P].[K] RDXARWSSOJYNLI-UHFFFAOYSA-N 0.000 description 1
- 239000011543 agarose gel Substances 0.000 description 1
- 238000007605 air drying Methods 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- 238000000137 annealing Methods 0.000 description 1
- 229910052787 antimony Inorganic materials 0.000 description 1
- 239000011324 bead Substances 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 238000004737 colorimetric analysis Methods 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 238000010219 correlation analysis Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 238000004925 denaturation Methods 0.000 description 1
- 230000036425 denaturation Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 238000005265 energy consumption Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 238000005048 flame photometry Methods 0.000 description 1
- 238000013467 fragmentation Methods 0.000 description 1
- 238000006062 fragmentation reaction Methods 0.000 description 1
- 239000000499 gel Substances 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 238000002386 leaching Methods 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 238000005453 pelletization Methods 0.000 description 1
- 239000000575 pesticide Substances 0.000 description 1
- 238000012257 pre-denaturation Methods 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000004064 recycling Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 239000008223 sterile water Substances 0.000 description 1
- 239000010902 straw Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/25—Root crops, e.g. potatoes, yams, beet or wasabi
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F5/00—Fertilisers from distillery wastes, molasses, vinasses, sugar plant or similar wastes or residues, e.g. from waste originating from industrial processing of raw material of agricultural origin or derived products thereof
- C05F5/002—Solid waste from mechanical processing of material, e.g. seed coats, olive pits, almond shells, fruit residue, rice hulls
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/10—Solid or semi-solid fertilisers, e.g. powders
- C05G5/12—Granules or flakes
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Pest Control & Pesticides (AREA)
- Engineering & Computer Science (AREA)
- Botany (AREA)
- Soil Sciences (AREA)
- Mechanical Engineering (AREA)
- Environmental & Geological Engineering (AREA)
- Environmental Sciences (AREA)
- Fertilizers (AREA)
Abstract
The invention relates to the field of plant nutrition conditioning agents, in particular to a preparation method and a use method of a plant nutrition conditioning agent based on marine oligomeric amino polysaccharide. The plant nutrition conditioner comprises the following components in parts by weight: 25-35 parts of bean pulp powder, 55-65 parts of humic acid, 0.5-2 parts of marine oligomeric micromolecular aminopolysaccaride and 5-15 parts of nitrogen-phosphorus-potassium compound fertilizer. The plant nutrition conditioner can promote the growth of ginseng seedlings, effectively improve the seedling storage rate, improve various growth indexes of ginseng, has remarkable effect of promoting the growth and is beneficial to the accumulation of dry matters; the richness of microorganisms according to soil is improved.
Description
Technical Field
The invention relates to the field of plant nutrition conditioning agents, in particular to a preparation method and a use method of a plant nutrition conditioning agent based on marine oligomeric amino polysaccharide.
Background
The electron flow irradiation is adopted to degrade the high molecular weight polysaccharide, and free radicals are generated in the system to attack the glycosidic bond so as to break the high molecular weight polysaccharide, thereby realizing the fragmentation of the high molecular weight polysaccharide, achieving the purpose of reducing the molecular weight and preparing the low molecular weight polysaccharide with excellent bioactivity.
The plant nutrition conditioner produced by electron flow irradiation has the defects of poor uniformity, poor product quality, large reagent consumption, high energy consumption, poor degradation effect, high cost and the like.
In view of this, the present invention has been made.
Disclosure of Invention
The invention relates to a plant nutrition conditioner based on marine oligomeric amino polysaccharide, which comprises the following components in parts by weight:
25-35 parts of bean pulp powder, 55-65 parts of humic acid, 0.5-2 parts of marine oligomeric micromolecular aminopolysaccaride and 5-15 parts of nitrogen-phosphorus-potassium compound fertilizer.
The plant nutrition conditioner based on the marine oligomeric amino polysaccharide can promote the growth of ginseng seedlings, effectively improve the seedling storage rate, improve various growth indexes of ginseng, has remarkable effect of promoting the growth, and is beneficial to the accumulation of dry matters; the richness of microorganisms according to soil is improved.
In another aspect, the invention also relates to a method for preparing the marine oligoaminopolysaccharide-based plant nutrition conditioner, which comprises the following steps:
the components are mixed and granulated.
The preparation method of the plant nutrition conditioner based on the marine oligomeric amino polysaccharide is simple and easy to operate, has low cost, and the prepared composite plant nutrition conditioner has stable property and good quality.
In another aspect, the invention also relates to a cultivation method of ginseng, and the plant nutrition conditioner based on the marine oligoaminopolysaccharide is used.
Compared with the prior art, the invention has the beneficial effects that:
(1) The plant nutrition conditioner based on the marine oligomeric amino polysaccharide provided by the invention has the advantages that all the components are synergistic according to a certain proportion, the plant nutrition conditioner based on the marine oligomeric amino polysaccharide with proper concentration can effectively promote the growth of ginseng seedlings, effectively improve the seedling storage rate, improve various growth indexes of ginseng, has obvious promotion effect and is beneficial to the accumulation of dry matters; the plant nutrition conditioner based on the marine oligomeric amino polysaccharide can obviously improve the physical and chemical factor contents of soil pH, organic matters, quick-acting potassium and the like; in the ginseng cultivation process, the plant nutrition conditioner based on the marine oligomeric amino polysaccharide is applied, so that the microbial flora quantity of rhizosphere soil is increased, and the microbial diversity is enriched.
(2) The preparation method of the plant nutrition conditioner based on the marine oligomeric amino polysaccharide adopts a disc granulation mode, improves the uniformity and the quality stability of the plant nutrition conditioner, and has low production cost, simplicity and easy operation.
(3) The invention provides a cultivation method of ginseng, and the cultivated ginseng has good quality and high yield.
Detailed Description
The technical solution of the present invention will be clearly and completely described in conjunction with the specific embodiments, but it will be understood by those skilled in the art that the examples described below are some, but not all, examples of the present invention, and are intended to be illustrative only and should not be construed as limiting the scope of the present invention. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention. The specific conditions are not noted in the examples and are carried out according to conventional conditions or conditions recommended by the manufacturer. The reagents or apparatus used were conventional products commercially available without the manufacturer's attention.
The invention relates to a plant nutrition conditioner based on marine oligomeric amino polysaccharide, which comprises the following components in parts by weight:
25 to 35 parts (such as 25 parts, 27 parts, 29 parts, 31 parts, 33 parts or 35 parts), 55 to 65 parts (such as 55 parts, 57 parts, 59 parts, 61 parts, 63 parts or 65 parts) of humic acid, 0.5 to 2 parts (such as 0.5 parts, 0.7 parts, 0.9 parts, 1.1 parts, 1.3 parts, 1.5 parts, 1.7 parts, 1.9 parts or 2 parts) of marine oligomeric small molecule aminopolysaccharide, and 5 to 15 parts (such as 5 parts, 7 parts, 9 parts, 11 parts, 13 parts or 15 parts) of nitrogen-phosphorus-potassium compound fertilizer.
The plant nutrition conditioner based on the marine oligomeric amino polysaccharide has the advantages that all the components are synergistic according to a certain proportion, the plant nutrition conditioner based on the marine oligomeric amino polysaccharide with proper concentration can effectively promote the growth of ginseng seedlings, effectively improve the seedling storage rate, improve various growth indexes of ginseng, has obvious promotion effect and is beneficial to the accumulation of dry matters; the plant nutrition conditioner based on the marine oligomeric amino polysaccharide can obviously improve the physical and chemical factor contents of soil pH, organic matters, quick-acting potassium and the like; in the ginseng cultivation process, the plant nutrition conditioner based on the marine oligomeric amino polysaccharide is applied, so that the microbial flora quantity of rhizosphere soil is increased, and the microbial diversity is enriched.
Preferably 28 to 33 parts of bean pulp powder, 58 to 63 parts of humic acid, 0.8 to 1.8 parts of marine oligomeric micromolecular aminopolysaccaride and 8 to 13 parts of nitrogen-phosphorus-potassium compound fertilizer.
Preferably, the organic matter nutrient content of the marine oligoaminopolysaccharide-based plant nutrient conditioner is 35wt% -45 wt% (e.g. 35wt%, 37wt%, 39wt%, 41wt%, 43wt% or 45 wt%).
Preferably, the particle size of the plant nutrition conditioner based on the marine oligomeric aminopolysaccharide is 4-5 mm.
Preferably, the marine oligomeric small molecule aminopolysaccharide has an atomic mass of 1.5 to 3kDa (e.g., 1.5kDa, 2kDa, 2.5kDa or 3 kDa).
Preferably, the mass ratio of nitrogen, phosphorus and potassium in the nitrogen-phosphorus-potassium compound fertilizer is 4-6: 1 to 3:1 to 2.
In another aspect, the invention also relates to a method for preparing the marine oligoaminopolysaccharide-based plant nutrition conditioner, which comprises the following steps:
the components are mixed and granulated.
The preparation method of the plant nutrition conditioner based on the marine oligomeric amino polysaccharide can improve the uniformity and the quality stability of the plant nutrition conditioner, and has low production cost, simplicity and easiness in operation.
Preferably, the means of granulation comprises disc granulation.
Preferably, the number of revolutions of the disk granulation is 15 to 25rpm/min (e.g., 15rpm/min, 17rpm/min, 19rpm/min, 21rpm/min, 23rpm/min, or 25 rpm/min).
Preferably, the disc inclination of the disc granulation is 45 ° ～55 ° (e.g. 45 ° 、47 ° 、49 ° 、51 ° 、53 ° Or 55 ° )。
Preferably, the granulating time is 35-45 min (35 min, 37min, 39min, 41min, 43min or 45 min).
In another aspect, the invention also relates to a cultivation method of ginseng, and the plant nutrition conditioner based on the marine oligoaminopolysaccharide is used.
Embodiments of the present invention will be described in detail below with reference to specific examples and comparative examples.
Example 1
The plant nutrition conditioner based on the marine oligomeric aminopolysaccharide provided by the embodiment comprises the following components in parts by mass:
35 parts of soybean meal powder, 55 parts of humic acid, 0.5 part of marine oligomeric micromolecular aminopolysaccaride and 15 parts of nitrogen-phosphorus-potassium compound fertilizer;
the particle size of the plant nutrition conditioner based on the marine oligomeric amino polysaccharide is 4.14mm; the atomic mass of the marine oligomeric micromolecular aminopolysaccaride is 1.5-3 kDa;
the components were mixed and granulated on a disk with a revolution of 20 revolutions per minute, a disk inclination of 50 °, and a time of 40 minutes.
Example 2
The plant nutrition conditioner based on the marine oligomeric aminopolysaccharide provided by the embodiment comprises the following components in parts by mass:
25 parts of soybean meal, 65 parts of humic acid, 2 parts of marine oligomeric micromolecular aminopolysaccharide and 15 parts of nitrogen-phosphorus-potassium compound fertilizer;
the particle size of the plant nutrition conditioner based on the marine oligomeric amino polysaccharide is 4mm; the atomic mass of the marine oligomeric micromolecular aminopolysaccaride is 1.5-3 kDa;
mixing the components, granulating with disc at 15rpm/min and disc inclination of 55 ° The time was 35min.
Example 3
The plant nutrition conditioner based on the marine oligomeric aminopolysaccharide provided by the embodiment comprises the following components in parts by mass:
30 parts of soybean meal, 60 parts of humic acid, 1 part of marine oligomeric micromolecular aminopolysaccharide and 9 parts of nitrogen-phosphorus-potassium compound fertilizer.
The particle size of the plant nutrition conditioner based on the marine oligomeric amino polysaccharide is 5mm; the atomic mass of the marine oligomeric micromolecular aminopolysaccaride is 1.5-3 kDa;
mixing the components, granulating with disc at 25rpm/min with disc inclination of 45 ° The time was 45min.
Comparative example 1
The plant nutrition conditioner based on the marine oligomeric aminopolysaccharide provided by the comparative example comprises the following components in parts by mass:
40 parts of soybean meal, 50 parts of humic acid, 3 parts of marine oligomeric micromolecular aminopolysaccharide and 13 parts of nitrogen-phosphorus-potassium compound fertilizer;
the procedure is as in example 3.
Comparative example 2
This comparative example differs from example 3 only in that the atomic mass of the marine oligomeric small molecule aminopolysaccharide is 1kDa.
Comparative example 3
The comparative example differs from example 3 only in that the number of revolutions for pelletization was 30rpm/min.
Experimental example
The soil is sieved by a 60-mesh sieve to remove impurities such as straw stones, and the like, and the soil is put into flowerpots with the diameter of 20cm, and each pot is filled with 2kg of soil. Adopting random block design, selecting annual ginseng seedlings with consistent size and good growth for transplanting, and setting 4 dosage gradients in pot experiments for 6 plants per pot: 0.3g/L, 0.6g/L, 1.2g/L and 2.4g/L, noted SAT1, SAT2, SAT3, SAT4, respectively, and sterile water control CK, 5 replicates per treatment. In addition, annual ginseng seedlings are planted by using the plant nutrition conditioning agents based on marine oligoaminopolysaccharide of the examples 1,2 and 3 respectively, and the use amount of the plant nutrition conditioning agents of each pot is 30g by taking the comparative examples 1,2 and 3 as a control and 6 plants of each pot. The ginseng potted plant is placed in a artificial climate chamber, the temperature is between 17 and 28 ℃, the relative humidity is between 70 and 80 percent, and all treatments are carried out by watering water once a week, and 200ml of water is filled in each pot.
And (3) from 11 months in 2020 to 3 months in 2021, cleaning the root soil of the ginseng after a growth period of the potted ginseng is finished, and respectively weighing fresh weights of the stem leaves and the root after airing the water. And then naturally air-drying to constant weight, and measuring the dry weight of the stem and the leaf and the dry weight of the root. Rhizosphere soil of each ginseng is collected, and the rhizosphere soil of each basin is mixed to be repeated once. And (3) reserving one part of the newly collected soil sample in an ultralow temperature refrigerator at the temperature of-80 ℃ for extracting and identifying the total genome of the soil microorganism, and sun-drying and recycling the other part for physical and chemical index analysis.
The method for measuring the physical and chemical indexes of the soil comprises the following steps: the pH value of the soil is measured by adopting a pH/ORP acidometer; the Total Carbon (TC) and Total Nitrogen (TN) contents of the soil are measured by a soil carbon nitrogen element analyzer; the content of alkaline hydrolysis nitrogen is measured by adopting a NaOH alkaline hydrolysis diffusion method; the total phosphorus is measured by adopting an alkali fusion-molybdenum-antimony colorimetric method; the effective phosphorus adopts NaHCO 3 Leaching method measurement; quick-acting potassium is measured by flame photometry.
Extraction of DNA and high throughput sequencing: extraction of nucleic acid was accomplished using TGuide S96 magnetic bead method soil genomic DNA extraction kit (tiangen biochemical technologies, DP 812). The DNA concentration was measured by a microplate reader (synergy HTX). The amplification and high throughput sequencing service of corresponding sections of the ribosome coding genes of fungi and bacteria are independently completed by the Baimichael biotechnology Co, and the amplification of the bacterial 16S rDNA V3+V4 sections is carried out, wherein the primers are 338F:5'-ACTCCTACGGGAGGCAGCA-3',806R:5'-GGACTACHVGGGTWTCTAAT-3'; amplifying fungus ITS segment, wherein the primer is ITS1F:5'-CTTGGTCATTTAGAGGAAGTAA-3', ITS2:5'-GCTGCGTTCTTCATCGATGC-3'. The PCR amplification reaction conditions were 1min at 95℃for pre-denaturation, 30s at 95℃for denaturation, 30s at 50℃for annealing, 40s at 72℃for extension, 25 cycles, and 7min at 72 ℃. The bands were detected by 1.8% agarose gel, purified using the e.Z.N.A.TM.cycle-Pure Kit (omega) DNA gel extraction Kit, and then quantified. The high throughput sequencing results were completed by end pair sequencing using the Illumina NovaSeq platform from beijing hundred michael biosystems, according to standard protocols.
Data statistics and analysis: data statistics and correlation analysis are completed through Excel 2013 and SAS 9.0 software processing; and (3) carrying out quality filtration on the Raw Reads obtained by sequencing, identifying and removing primer sequences by using Cutadapts (V1.9.1), splicing double-end sequences by using overlap, identifying and removing chimeras by using UCHIME V8.1 software, and obtaining high-quality data. Clustering analysis (UP-ARSE pipeline, V7.0) was performed according to 97% similarity, and the clustering results were combined with species annotation, with the final results showing the OTUS and classification pedigree of the samples. Alpha diversity index analysis: calculating diversity indexes such as Shannon, chao1 and the like through software QIIME 2; performing redundancy analysis of physicochemical factors and microorganism diversity by software Canoco5.0; speSS 23.0 software was used for Spearman correlation analysis.
After the ginseng is treated by using the marine oligomeric aminopolysaccharide and the marine oligomeric aminopolysaccharide organic fertilizer, the aminopolysaccharide with proper concentration can effectively promote the growth of ginseng seedlings, effectively improve the seedling storage rate, improve various growth indexes of the ginseng, has obvious promotion effect and is beneficial to the accumulation of dry matters; the optimum use concentration of marine oligomeric amino polysaccharides is 0.6g/L, and the best example of organic fertilizer is the plant nutrient conditioner based on marine oligomeric amino polysaccharides of example 3.
In ginseng soil index analysis, the physical and chemical factors of the soil are obviously changed, the change of the environmental factors further affects the diversity and the functional characteristics of soil microbial communities, and Shi Anji polysaccharide is obviously changed in comparison with a control for total nitrogen content, organic matters, ammonium nitrogen, nitrate nitrogen, quick-acting phosphorus potassium content and the like of the soil, wherein the physical and chemical factors of the soil such as pH, organic matters, quick-acting potassium and the like are obviously improved; but the different concentrations have different degrees of change on the physicochemical properties of the soil.
In the ginseng cultivation process, under the treatment of aminopolysaccharide with different concentrations, the application of aminopolysaccharide improves the microbial flora quantity of rhizosphere soil and enriches microbial diversity, wherein the influence of the application of aminopolysaccharide with different concentrations on the microbial flora quantity is different. Five treatments at different concentrations yielded a total of 1732 bacterial OTUs, belonging to 28 phylum, 78 class, 170 mesh, 255 family, 374 genus and 395 species. The dominant phylum consisting of soil bacterial communities is in turn: proteobacteria, actinomycetes, bacillus, rhizopus and Acidobacter, etc. Wherein, the aminopolysaccharide has larger influence on bacteria of Proteobacteria, and OUT is obviously higher than that of a control group; OTU of actinomycota bacteria was significantly inhibited. The fungal communities are classified in 1412 fungal OTUs, belonging to 12 phylum, 35 classes, 82 orders, 163 families, 343 genera and 456 species. The dominant mycota consisting of the soil fungus community treated by the aminopolysaccharide is ascomycota, basidiomycota, mortierella and Pot fungus in sequence, and the dominant mycota accounts for 86.96-90.76% of the total abundance. Compared with CK, the abundance of Ascomycota is obviously reduced after polysaccharide is added, and the abundance of Basidiomycota, chytridiomycota fungi is improved. The ginseng cultivation soil microbial community treated by the aminopolysaccharide with different concentrations has different structures, and the change of the status and diversity of the ginseng soil functional microorganisms applying the aminopolysaccharide is related to the soil environmental factors; the method changes the physicochemical property of the soil, is beneficial to the growth of some beneficial microorganisms, and is a reserve resource for developing biological pesticides or natural fertilizers on ginseng on the premise of improving the soil and changing the diversity of microbial communities. The research result can provide theoretical basis for ginseng field cultivation, and has practical biological significance for improving ginseng cultivation soil microorganisms.
Three places are selected and are respectively positioned at the left town of Jilin city, the Dunzhu city, the Renhe river and the small wasteland, the implementation is carried outThe marine oligoaminopolysaccharide-based phytonutrient conditioner of example 3 was applied to three lands, each at 250g/m 3 、500g/m 3 、1000g/m 3 The three different application amounts are respectively named as DT1, DT2 and DT3, different indexes of ammonium nitrogen, nitrate nitrogen, quick-acting phosphorus, quick-acting potassium, pH value, salinity and organic matter 7 are measured, the measured data are shown in tables 1,2 and 3, the content detected by DT1 in the three places of the indexes of ammonium nitrogen, nitrate nitrogen, quick-acting phosphorus, quick-acting potassium and organic matter is higher than that of DT2 and DT3, the salt content of DT1 is lower than that of DT2 and DT3, the salt content of DT3 is lower than that of the control, each treatment of PH shows acidity, and the pH value of DT1 is lower than that of DT2 and DT 3. In summary, the optimal application concentration of the plant nutrition conditioner is 250g/m 3 。
TABLE 1
TABLE 2
TABLE 3 Table 3
While the invention has been illustrated and described with reference to specific embodiments, it is to be understood that the above embodiments are merely illustrative of the technical aspects of the invention and not restrictive thereof; those of ordinary skill in the art will appreciate that: modifications may be made to the technical solutions described in the foregoing embodiments, or equivalents may be substituted for some or all of the technical features thereof, without departing from the spirit and scope of the present invention; such modifications and substitutions do not depart from the spirit of the corresponding technical solutions; it is therefore intended to cover in the appended claims all such alternatives and modifications as fall within the scope of the invention.
Claims (10)
1. The plant nutrition conditioner based on the marine oligomeric amino polysaccharide is characterized by comprising the following components in parts by weight:
25-35 parts of bean pulp powder, 55-65 parts of humic acid, 0.5-2 parts of marine oligomeric micromolecular aminopolysaccaride and 5-15 parts of nitrogen-phosphorus-potassium compound fertilizer.
2. The plant nutrition conditioner based on marine oligomeric amino polysaccharide according to claim 1, wherein 28-33 parts of soybean meal powder, 58-63 parts of humic acid, 0.8-1.8 parts of marine oligomeric small molecular amino polysaccharide and 8-13 parts of nitrogen-phosphorus-potassium compound fertilizer.
3. The marine oligoaminopolysaccharide based plant nutrient conditioner according to claim 1 or 2, wherein the marine oligoaminopolysaccharide based plant nutrient conditioner has an organic matter nutrient content of 35wt% to 45wt%;
preferably, the particle size of the plant nutrition conditioner based on the marine oligomeric aminopolysaccharide is 4-5 mm.
4. The plant nutrient conditioner based on marine oligoaminopolysaccharide according to claim 1 or 2, wherein the atomic mass of the marine oligosmall molecular aminopolysaccharide is 1.5-3 kDa.
5. The marine oligoaminopolysaccharide-based plant nutrient conditioner according to claim 1 or 2, wherein the mass ratio of nitrogen, phosphorus and potassium in the nitrogen-phosphorus-potassium compound fertilizer is 4-6: 1 to 3:1 to 2.
6. A method for preparing a marine oligoaminopolysaccharide based plant nutrition conditioner according to any one of claims 1 to 5 comprising the steps of:
the components are mixed and granulated.
7. The method for preparing a marine oligoaminopolysaccharide based plant nutrient conditioner of claim 6 wherein the means of granulation comprises disc granulation.
8. The method for preparing a marine oligoaminopolysaccharide based plant nutrient conditioner according to claim 7, wherein the number of revolutions of the disc granulation is 15-25 rpm/min;
preferably, the disc inclination of the disc granulation is 45 ° ～55 ° 。
9. The method for preparing a plant nutrition conditioner based on marine oligo-aminopolysaccharide according to claim 7, wherein the granulating time is 35-45 min.
10. A method for cultivating ginseng, characterized in that the marine oligoaminopolysaccharide-based plant nutrient conditioner as claimed in any one of claims 1 to 5 is used.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310874403.8A CN117126016B (en) | 2023-07-17 | 2023-07-17 | Preparation and application methods of plant nutrition conditioner based on marine oligomeric amino polysaccharide |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310874403.8A CN117126016B (en) | 2023-07-17 | 2023-07-17 | Preparation and application methods of plant nutrition conditioner based on marine oligomeric amino polysaccharide |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN117126016A true CN117126016A (en) | 2023-11-28 |
| CN117126016B CN117126016B (en) | 2024-07-05 |
Family
ID=88861824
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202310874403.8A Active CN117126016B (en) | 2023-07-17 | 2023-07-17 | Preparation and application methods of plant nutrition conditioner based on marine oligomeric amino polysaccharide |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN117126016B (en) |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103387453A (en) * | 2013-07-23 | 2013-11-13 | 苏州仁成生物科技有限公司 | Traditional Chinese medicine fertilizer for ginseng and preparation method thereof |
| CN106833668A (en) * | 2016-12-30 | 2017-06-13 | 中国农业科学院农业环境与可持续发展研究所 | A kind of mineral microorganism soil conditioner and its production and use |
| CN108218574A (en) * | 2018-01-30 | 2018-06-29 | 金华市农业科学研究院 | A kind of fertilizer packet and its application method |
| CN110235723A (en) * | 2018-03-10 | 2019-09-17 | 重庆康调农业科技有限公司 | A kind of implantation methods of high-quality Chinese yam |
| CN114436697A (en) * | 2022-02-10 | 2022-05-06 | 桂林长发小寨生物科技有限公司 | Special ginseng organic fertilizer for rice |
| CN114621039A (en) * | 2020-12-11 | 2022-06-14 | 中国科学院大连化学物理研究所 | Application of polysaccharide as soil conditioner |
| CN115724698A (en) * | 2022-11-30 | 2023-03-03 | 中昇南阳生物科技有限公司 | Acidic soil conditioner and preparation method thereof |
-
2023
- 2023-07-17 CN CN202310874403.8A patent/CN117126016B/en active Active
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103387453A (en) * | 2013-07-23 | 2013-11-13 | 苏州仁成生物科技有限公司 | Traditional Chinese medicine fertilizer for ginseng and preparation method thereof |
| CN106833668A (en) * | 2016-12-30 | 2017-06-13 | 中国农业科学院农业环境与可持续发展研究所 | A kind of mineral microorganism soil conditioner and its production and use |
| CN108218574A (en) * | 2018-01-30 | 2018-06-29 | 金华市农业科学研究院 | A kind of fertilizer packet and its application method |
| CN110235723A (en) * | 2018-03-10 | 2019-09-17 | 重庆康调农业科技有限公司 | A kind of implantation methods of high-quality Chinese yam |
| CN114621039A (en) * | 2020-12-11 | 2022-06-14 | 中国科学院大连化学物理研究所 | Application of polysaccharide as soil conditioner |
| CN114436697A (en) * | 2022-02-10 | 2022-05-06 | 桂林长发小寨生物科技有限公司 | Special ginseng organic fertilizer for rice |
| CN115724698A (en) * | 2022-11-30 | 2023-03-03 | 中昇南阳生物科技有限公司 | Acidic soil conditioner and preparation method thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN117126016B (en) | 2024-07-05 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Fu et al. | Dynamics of bacterial and eukaryotic community associated with stability during vermicomposting of pelletized dewatered sludge | |
| Zhu et al. | Associative effectiveness of bio-organic fertilizer and soil conditioners derived from the fermentation of food waste applied to greenhouse saline soil in Shan Dong Province, China | |
| Li et al. | The use of mercapto-modified palygorskite prevents the bioaccumulation of cadmium in wheat | |
| Wang et al. | Halophyte planting improves saline-alkali soil and brings changes in physical and chemical properties and soil microbial communities | |
| CN110846262B (en) | Serratia marcescens SZ201 and application thereof | |
| CN113174334A (en) | Method for screening simplified flora of sugarcane rhizosphere growth-promoting bacteria | |
| CN117701479B (en) | Bacterial strain capable of degrading benzonitrile herbicide, microbial agent and application thereof | |
| Zhang et al. | Biochar and Chlorella increase rice yield by improving saline-alkali soil physicochemical properties and regulating bacteria under aquaculture wastewater irrigation | |
| Yang et al. | Co-application of biochar and microbial inoculants increases soil phosphorus and potassium fertility and improves soil health and tomato growth | |
| CN117126016B (en) | Preparation and application methods of plant nutrition conditioner based on marine oligomeric amino polysaccharide | |
| Wang et al. | Organic Fertilizers and Soil Conditioner Recover Chemical Fertilizer-Induced Changes in Soil Bacterial Community Diversity in Wine Grape Rhizosphere Soil. | |
| CN109232136A (en) | A kind of production method of pawpaw organic fertilizer | |
| Wang et al. | Biodegradable microplastics pose greater risks than conventional microplastics to soil properties, microbial community and plant growth, especially under flooded conditions | |
| CN108866041B (en) | Extraction method of guava phyllospheric microorganism genome DNA | |
| CN112501039B (en) | Methodactis bijianensis and method for treating high-salt wastewater | |
| Wang et al. | Influence of Fertilizers and Soil Conditioners on Soil Bacterial Diversity and the Quality of Wine Grape (Cabernet Sauvignon). | |
| JP7485272B2 (en) | Plant Growth Promoters | |
| CN114480207A (en) | Pacific bacillus and application thereof in degradation of sulfide in sewage wastewater | |
| Khdir et al. | Combined Effect of Biochar and Mycorrhizal Fungi on Wheat (Triticum aestivum L.) Growth and Performance in Calcareous Soil | |
| CN108823207B (en) | Bn-miR43 of ramie and application thereof | |
| CN113817608A (en) | Separation and enrichment method of microorganisms on surface of alcoholized tobacco leaf | |
| EP3887343A1 (en) | Process for the manufacture of a biostimulant or natural fertilizer based on fermented macroalgae, natural additives and probiotic bacteria for agriculture and horticulture | |
| Li et al. | Effect of humic acid and bacterial manure on distribution of heavy metals in different organs of maize | |
| CN116970525B (en) | Lactic acid bacteria in-situ enrichment bead and preparation method thereof | |
| RU2580469C2 (en) | Method of producing liquid humic organo-mineral fertiliser for plants |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |